Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044243.t1 | GAU51471.1 | 46.2 | 106 | 53 | 1 | 22 | 127 | 90 | 191 | 4.80E-15 | 90.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044243.t1 | A0A2Z6P4W7 | 46.2 | 106 | 53 | 1 | 22 | 127 | 90 | 191 | 3.5e-15 | 90.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044243 | MS.gene77466 | PPI |
| MS.gene044243 | MS.gene044361 | PPI |
| MS.gene044243 | MS.gene71282 | PPI |
| MS.gene044243 | MS.gene71524 | PPI |
| MS.gene044243 | MS.gene71528 | PPI |
| MS.gene044243 | MS.gene70424 | PPI |
| MS.gene044243 | MS.gene77464 | PPI |
| MS.gene77472 | MS.gene044243 | PPI |
| MS.gene044243 | MS.gene044326 | PPI |
| MS.gene044243 | MS.gene79115 | PPI |
| MS.gene044243 | MS.gene70427 | PPI |
| MS.gene044243 | MS.gene70418 | PPI |
| MS.gene044243 | MS.gene71531 | PPI |
| MS.gene044243 | MS.gene049263 | PPI |
| MS.gene044243 | MS.gene044370 | PPI |
| MS.gene044243 | MS.gene70476 | PPI |
| MS.gene044243 | MS.gene25704 | PPI |
| MS.gene044243 | MS.gene77468 | PPI |
| MS.gene044243 | MS.gene044323 | PPI |
| MS.gene044363 | MS.gene044243 | PPI |
| MS.gene044243 | MS.gene71284 | PPI |
| MS.gene044243 | MS.gene044376 | PPI |
| MS.gene044243 | MS.gene70443 | PPI |
| MS.gene044243 | MS.gene70430 | PPI |
| MS.gene044243 | MS.gene045214 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044243.t1 | MTR_3g083080 | 43.182 | 132 | 64 | 3 | 5 | 130 | 57 | 183 | 1.06e-24 | 94.0 |
| MS.gene044243.t1 | MTR_3g080680 | 42.568 | 148 | 62 | 6 | 5 | 132 | 33 | 177 | 6.63e-21 | 84.0 |
| MS.gene044243.t1 | MTR_3g083050 | 43.902 | 123 | 61 | 3 | 2 | 121 | 59 | 176 | 5.35e-20 | 81.6 |
| MS.gene044243.t1 | MTR_3g083030 | 43.902 | 123 | 61 | 3 | 2 | 121 | 82 | 199 | 6.91e-20 | 82.0 |
| MS.gene044243.t1 | MTR_3g082420 | 40.777 | 103 | 58 | 2 | 33 | 132 | 97 | 199 | 7.64e-18 | 76.3 |
| MS.gene044243.t1 | MTR_3g082340 | 40.777 | 103 | 58 | 2 | 33 | 132 | 98 | 200 | 2.27e-17 | 75.1 |
| MS.gene044243.t1 | MTR_4g055800 | 38.532 | 109 | 56 | 3 | 28 | 130 | 171 | 274 | 2.45e-14 | 68.2 |
| MS.gene044243.t1 | MTR_3g083150 | 36.232 | 138 | 75 | 5 | 4 | 131 | 57 | 191 | 2.21e-12 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTCTGAGCTTGCTTTGA+AGG | 0.280067 | 3.3:-68279024 | MS.gene044243:CDS |
| GTCAGCAATTAGTTCTTCTA+TGG | 0.315331 | 3.3:+68279271 | None:intergenic |
| GAGAGTAGGGCGAAGATCTT+CGG | 0.406860 | 3.3:+68279052 | None:intergenic |
| GTTGTTAAGTTGTTATAGCA+TGG | 0.435461 | 3.3:+68278771 | None:intergenic |
| GTTCGACGATGACGACTTTG+CGG | 0.439069 | 3.3:-68279306 | MS.gene044243:CDS |
| GTCAAGTGTACCACTGCTAT+TGG | 0.447420 | 3.3:-68278860 | MS.gene044243:CDS |
| GGATTACAATCAAAAGAATC+AGG | 0.463360 | 3.3:-68279003 | MS.gene044243:intron |
| GTCGTCATCGTCATCGTCCA+TGG | 0.476185 | 3.3:+68279349 | None:intergenic |
| ACTGCTATTGGTTGTGGTGG+TGG | 0.480634 | 3.3:-68278848 | MS.gene044243:CDS |
| CAGAGAGAGTAACGAGAGTA+GGG | 0.497560 | 3.3:+68279039 | None:intergenic |
| TCAGAGAGAGTAACGAGAGT+AGG | 0.497887 | 3.3:+68279038 | None:intergenic |
| CCACATAACTCTAGAACGGC+TGG | 0.510258 | 3.3:+68279098 | None:intergenic |
| TTGTTAAGTTGTTATAGCAT+GGG | 0.514294 | 3.3:+68278772 | None:intergenic |
| TGTACCACTGCTATTGGTTG+TGG | 0.522647 | 3.3:-68278854 | MS.gene044243:CDS |
| AAGATCTTCGGTTATGTCAA+AGG | 0.527327 | 3.3:+68279064 | None:intergenic |
| AGTTGTTATAGCATGGGTGT+TGG | 0.535950 | 3.3:+68278778 | None:intergenic |
| CGACTTTGCGGAAGATAAAG+AGG | 0.546436 | 3.3:-68279294 | MS.gene044243:CDS |
| AGATCTTCGGTTATGTCAAA+GGG | 0.549668 | 3.3:+68279065 | None:intergenic |
| GTTATAGCATGGGTGTTGGA+AGG | 0.561860 | 3.3:+68278782 | None:intergenic |
| ACCACTGCTATTGGTTGTGG+TGG | 0.575309 | 3.3:-68278851 | MS.gene044243:CDS |
| CCAGCCGTTCTAGAGTTATG+TGG | 0.626689 | 3.3:-68279098 | MS.gene044243:CDS |
| ACAGCCACATAACTCTAGAA+CGG | 0.642024 | 3.3:+68279094 | None:intergenic |
| TAGCAGTGGTACACTTGACA+AGG | 0.661404 | 3.3:+68278864 | None:intergenic |
| GGATGATAGATAGCATCGAA+CGG | 0.667704 | 3.3:+68279119 | None:intergenic |
| ACCACCACAACCAATAGCAG+TGG | 0.671651 | 3.3:+68278850 | None:intergenic |
| AAAGTCGTCATCGTCGAACG+TGG | 0.686780 | 3.3:+68279310 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCTTCTTCTTAATTATTA+GGG | - | chr3.3:68279214-68279233 | MS.gene044243:intron | 20.0% |
| !! | TGAAACATGATAAAATTGAA+TGG | + | chr3.3:68278950-68278969 | None:intergenic | 20.0% |
| !! | TGTTCTTCTTCTTAATTATT+AGG | - | chr3.3:68279213-68279232 | MS.gene044243:intron | 20.0% |
| ! | CAATTATTCATCAACTAATC+TGG | - | chr3.3:68279180-68279199 | MS.gene044243:intron | 25.0% |
| AAAGATAGATTCGTACACTT+AGG | + | chr3.3:68278897-68278916 | None:intergenic | 30.0% | |
| GGATTACAATCAAAAGAATC+AGG | - | chr3.3:68279119-68279138 | MS.gene044243:CDS | 30.0% | |
| AAGATCTTCGGTTATGTCAA+AGG | + | chr3.3:68279061-68279080 | None:intergenic | 35.0% | |
| AGATCTTCGGTTATGTCAAA+GGG | + | chr3.3:68279060-68279079 | None:intergenic | 35.0% | |
| ! | GTCAGCAATTAGTTCTTCTA+TGG | + | chr3.3:68278854-68278873 | None:intergenic | 35.0% |
| !! | TTCTTCTGCTTTTGCTTGAA+AGG | + | chr3.3:68279317-68279336 | None:intergenic | 35.0% |
| AGTTGTTATAGCATGGGTGT+TGG | + | chr3.3:68279347-68279366 | None:intergenic | 40.0% | |
| ! | ACAGCCACATAACTCTAGAA+CGG | + | chr3.3:68279031-68279050 | None:intergenic | 40.0% |
| ! | GGATGATAGATAGCATCGAA+CGG | + | chr3.3:68279006-68279025 | None:intergenic | 40.0% |
| CAGAGAGAGTAACGAGAGTA+GGG | + | chr3.3:68279086-68279105 | None:intergenic | 45.0% | |
| CGACTTTGCGGAAGATAAAG+AGG | - | chr3.3:68278828-68278847 | MS.gene044243:CDS | 45.0% | |
| GTCAAGTGTACCACTGCTAT+TGG | - | chr3.3:68279262-68279281 | MS.gene044243:CDS | 45.0% | |
| TAGCAGTGGTACACTTGACA+AGG | + | chr3.3:68279261-68279280 | None:intergenic | 45.0% | |
| TCAGAGAGAGTAACGAGAGT+AGG | + | chr3.3:68279087-68279106 | None:intergenic | 45.0% | |
| ! | TCTCTCTGAGCTTGCTTTGA+AGG | - | chr3.3:68279098-68279117 | MS.gene044243:CDS | 45.0% |
| ! | TGTACCACTGCTATTGGTTG+TGG | - | chr3.3:68279268-68279287 | MS.gene044243:CDS | 45.0% |
| !! | GTTATAGCATGGGTGTTGGA+AGG | + | chr3.3:68279343-68279362 | None:intergenic | 45.0% |
| AAAGTCGTCATCGTCGAACG+TGG | + | chr3.3:68278815-68278834 | None:intergenic | 50.0% | |
| ACCACCACAACCAATAGCAG+TGG | + | chr3.3:68279275-68279294 | None:intergenic | 50.0% | |
| GAGAGTAGGGCGAAGATCTT+CGG | + | chr3.3:68279073-68279092 | None:intergenic | 50.0% | |
| GTTCGACGATGACGACTTTG+CGG | - | chr3.3:68278816-68278835 | MS.gene044243:CDS | 50.0% | |
| ! | ACCACTGCTATTGGTTGTGG+TGG | - | chr3.3:68279271-68279290 | MS.gene044243:CDS | 50.0% |
| ! | ACTGCTATTGGTTGTGGTGG+TGG | - | chr3.3:68279274-68279293 | MS.gene044243:CDS | 50.0% |
| !! | CCACATAACTCTAGAACGGC+TGG | + | chr3.3:68279027-68279046 | None:intergenic | 50.0% |
| !! | CCAGCCGTTCTAGAGTTATG+TGG | - | chr3.3:68279024-68279043 | MS.gene044243:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 68278775 | 68279369 | 68278775 | ID=MS.gene044243 |
| chr3.3 | mRNA | 68278775 | 68279369 | 68278775 | ID=MS.gene044243.t1;Parent=MS.gene044243 |
| chr3.3 | exon | 68279244 | 68279369 | 68279244 | ID=MS.gene044243.t1.exon1;Parent=MS.gene044243.t1 |
| chr3.3 | CDS | 68279244 | 68279369 | 68279244 | ID=cds.MS.gene044243.t1;Parent=MS.gene044243.t1 |
| chr3.3 | exon | 68279004 | 68279141 | 68279004 | ID=MS.gene044243.t1.exon2;Parent=MS.gene044243.t1 |
| chr3.3 | CDS | 68279004 | 68279141 | 68279004 | ID=cds.MS.gene044243.t1;Parent=MS.gene044243.t1 |
| chr3.3 | exon | 68278775 | 68278909 | 68278775 | ID=MS.gene044243.t1.exon3;Parent=MS.gene044243.t1 |
| chr3.3 | CDS | 68278775 | 68278909 | 68278775 | ID=cds.MS.gene044243.t1;Parent=MS.gene044243.t1 |
| Gene Sequence |
| Protein sequence |
