Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044282.t1 | XP_003601436.2 | 72.4 | 199 | 35 | 3 | 1 | 180 | 1 | 198 | 1.50E-70 | 275.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044282.t1 | G7J7C7 | 72.4 | 199 | 35 | 3 | 1 | 180 | 1 | 198 | 1.1e-70 | 275.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene70418 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene049263 | PPI |
| MS.gene044282 | MS.gene70476 | PPI |
| MS.gene044282 | MS.gene77468 | PPI |
| MS.gene044282 | MS.gene71531 | PPI |
| MS.gene044282 | MS.gene044323 | PPI |
| MS.gene77472 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene71284 | PPI |
| MS.gene77466 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene045214 | PPI |
| MS.gene25704 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene71282 | PPI |
| MS.gene044282 | MS.gene71524 | PPI |
| MS.gene044282 | MS.gene71528 | PPI |
| MS.gene044361 | MS.gene044282 | PPI |
| MS.gene79115 | MS.gene044282 | PPI |
| MS.gene044370 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene77464 | PPI |
| MS.gene70443 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene044326 | PPI |
| MS.gene044376 | MS.gene044282 | PPI |
| MS.gene044363 | MS.gene044282 | PPI |
| MS.gene044282 | MS.gene70427 | PPI |
| MS.gene044282 | MS.gene70424 | PPI |
| MS.gene70430 | MS.gene044282 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044282.t1 | MTR_3g080680 | 72.362 | 199 | 35 | 3 | 1 | 180 | 1 | 198 | 1.07e-97 | 281 |
| MS.gene044282.t1 | MTR_3g082420 | 46.154 | 156 | 79 | 3 | 28 | 180 | 68 | 221 | 3.27e-35 | 123 |
| MS.gene044282.t1 | MTR_3g082340 | 46.154 | 156 | 79 | 3 | 28 | 180 | 69 | 222 | 6.90e-35 | 122 |
| MS.gene044282.t1 | MTR_8g461300 | 45.930 | 172 | 77 | 6 | 20 | 180 | 32 | 198 | 1.50e-34 | 121 |
| MS.gene044282.t1 | MTR_3g083080 | 41.304 | 184 | 93 | 8 | 5 | 181 | 31 | 206 | 2.96e-26 | 100 |
| MS.gene044282.t1 | MTR_4g055800 | 40.000 | 150 | 71 | 5 | 20 | 164 | 138 | 273 | 2.77e-23 | 94.4 |
| MS.gene044282.t1 | MTR_3g083030 | 39.037 | 187 | 94 | 9 | 3 | 180 | 54 | 229 | 3.65e-22 | 90.1 |
| MS.gene044282.t1 | MTR_3g083050 | 40.341 | 176 | 85 | 9 | 14 | 180 | 42 | 206 | 4.03e-22 | 89.4 |
| MS.gene044282.t1 | MTR_3g083150 | 38.235 | 170 | 94 | 6 | 17 | 180 | 47 | 211 | 2.91e-17 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAACAGAAACAGGTGAAAA+AGG | 0.230480 | 3.3:+68799529 | MS.gene044282:CDS |
| TCTGATCTCAGGTGGTTTAA+TGG | 0.243396 | 3.3:-68800266 | None:intergenic |
| TTACCACGTTTGCCGTAATA+AGG | 0.262079 | 3.3:-68799623 | None:intergenic |
| AATAGATTCTACCATTAATA+AGG | 0.262811 | 3.3:-68800126 | None:intergenic |
| TGTACCCATGCCCTTATTAA+TGG | 0.282280 | 3.3:+68800115 | MS.gene044282:CDS |
| ATTAAAATTAAATGTGATCT+TGG | 0.329794 | 3.3:-68799852 | None:intergenic |
| TGGAGTGTCCTTCGAGAATA+GGG | 0.410247 | 3.3:-68799914 | None:intergenic |
| ACAGAGAATGGAACAGAAAC+AGG | 0.416228 | 3.3:+68799520 | None:intergenic |
| GGCTCAAATTCAAATCTATA+CGG | 0.426055 | 3.3:+68799682 | MS.gene044282:CDS |
| TTGGAGTGTCCTTCGAGAAT+AGG | 0.428317 | 3.3:-68799915 | None:intergenic |
| AAAGGGATCGGGATCGGGAT+TGG | 0.430515 | 3.3:+68799547 | MS.gene044282:CDS |
| AGTATAAGAATGAGGCATAT+AGG | 0.441135 | 3.3:+68799651 | MS.gene044282:CDS |
| GATTCCGACTTTCCTTATTA+CGG | 0.458848 | 3.3:+68799611 | MS.gene044282:CDS |
| GCTTGGAAAGTTGTTAAAGC+AGG | 0.466031 | 3.3:-68800196 | None:intergenic |
| ATAGATTCTACCATTAATAA+GGG | 0.476198 | 3.3:-68800125 | None:intergenic |
| GAGAATAGGGCGAACATCAT+CGG | 0.483809 | 3.3:-68799901 | None:intergenic |
| GTACGTCACAAGATCTTCAA+TGG | 0.490169 | 3.3:-68799579 | None:intergenic |
| GTGGCACACTCTCTGATCTC+AGG | 0.491079 | 3.3:-68800277 | None:intergenic |
| TTCTACCATTAATAAGGGCA+TGG | 0.508172 | 3.3:-68800120 | None:intergenic |
| AGGCCATGAAACACAAAAGC+TGG | 0.508489 | 3.3:-68800085 | None:intergenic |
| ATTAAATGTGATCTTGGTGG+AGG | 0.515471 | 3.3:-68799846 | None:intergenic |
| AGGACACTCCAAGCTTGCAT+TGG | 0.517715 | 3.3:+68799926 | MS.gene044282:CDS |
| AAAATTAAATGTGATCTTGG+TGG | 0.523290 | 3.3:-68799849 | None:intergenic |
| GTAATAGTCCAATGCAAGCT+TGG | 0.525102 | 3.3:-68799934 | None:intergenic |
| TACGTCACAAGATCTTCAAT+GGG | 0.528436 | 3.3:-68799578 | None:intergenic |
| TGATCTTGGTGGAGGGGCGA+TGG | 0.538731 | 3.3:-68799838 | None:intergenic |
| ATGGCACACTCTTTGACCAC+AGG | 0.540193 | 3.3:-68800247 | None:intergenic |
| CTTGGAAAGTTGTTAAAGCA+GGG | 0.540365 | 3.3:-68800195 | None:intergenic |
| TTTCCTTATTACGGCAAACG+TGG | 0.562249 | 3.3:+68799620 | MS.gene044282:CDS |
| GAACATCATCGGATATGTGA+AGG | 0.588800 | 3.3:-68799890 | None:intergenic |
| GAACACAGCATACTTCAGCT+TGG | 0.589049 | 3.3:-68800213 | None:intergenic |
| AACATCATCGGATATGTGAA+GGG | 0.592088 | 3.3:-68799889 | None:intergenic |
| TCTACCATTAATAAGGGCAT+GGG | 0.593250 | 3.3:-68800119 | None:intergenic |
| TAAATGTGATCTTGGTGGAG+GGG | 0.597056 | 3.3:-68799844 | None:intergenic |
| AGTTGTTAAAGCAGGGGTGT+CGG | 0.599691 | 3.3:-68800188 | None:intergenic |
| TTAAATGTGATCTTGGTGGA+GGG | 0.609839 | 3.3:-68799845 | None:intergenic |
| GCACACTCTCTGATCTCAGG+TGG | 0.621362 | 3.3:-68800274 | None:intergenic |
| TTGGAAAGTTGTTAAAGCAG+GGG | 0.623852 | 3.3:-68800194 | None:intergenic |
| GATGTTCGCCCTATTCTCGA+AGG | 0.637823 | 3.3:+68799906 | MS.gene044282:CDS |
| GGGCATGGGTACACTTGACA+AGG | 0.652935 | 3.3:-68800105 | None:intergenic |
| TTTGCCGTAATAAGGAAAGT+CGG | 0.657780 | 3.3:-68799615 | None:intergenic |
| ACATCATCGGATATGTGAAG+GGG | 0.660369 | 3.3:-68799888 | None:intergenic |
| TGAGGCATATAGGATGCTTG+AGG | 0.663425 | 3.3:+68799661 | MS.gene044282:CDS |
| TGTTCGAGACAAACAACCTG+TGG | 0.674826 | 3.3:+68800231 | MS.gene044282:CDS |
| TGGAGTGTATTAGATTGTAG+TGG | 0.705380 | 3.3:-68800296 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCTTCATTATATTATATTA+GGG | + | chr3.3:68800061-68800080 | MS.gene044282:intron | 10.0% |
| !! | AAAAACAAAATTGAAATAGA+TGG | - | chr3.3:68799753-68799772 | None:intergenic | 15.0% |
| !! | ATTAAAATTAAATGTGATCT+TGG | - | chr3.3:68799855-68799874 | None:intergenic | 15.0% |
| !! | CTTCTTCATTATATTATATT+AGG | + | chr3.3:68800060-68800079 | MS.gene044282:intron | 15.0% |
| !!! | AAGATCACATTTAATTTTAA+TGG | + | chr3.3:68799854-68799873 | MS.gene044282:CDS | 15.0% |
| !!! | AGATCACATTTAATTTTAAT+GGG | + | chr3.3:68799855-68799874 | MS.gene044282:CDS | 15.0% |
| !! | AATAGATTCTACCATTAATA+AGG | - | chr3.3:68800129-68800148 | None:intergenic | 20.0% |
| !! | AGAAGAACAATTAATTAGTT+TGG | - | chr3.3:68800045-68800064 | None:intergenic | 20.0% |
| !! | ATAGATTCTACCATTAATAA+GGG | - | chr3.3:68800128-68800147 | None:intergenic | 20.0% |
| !!! | TAATTTTGAGTATAAGAATG+AGG | + | chr3.3:68799643-68799662 | MS.gene044282:CDS | 20.0% |
| ! | AAAATTAAATGTGATCTTGG+TGG | - | chr3.3:68799852-68799871 | None:intergenic | 25.0% |
| ! | ATAGATAGATGTTTACACTT+AGG | - | chr3.3:68799721-68799740 | None:intergenic | 25.0% |
| !!! | CCTGATTATTGTTTTTTTCA+CGG | - | chr3.3:68799959-68799978 | None:intergenic | 25.0% |
| !!! | TCACATTTAATTTTAATGGG+CGG | + | chr3.3:68799858-68799877 | MS.gene044282:CDS | 25.0% |
| !!! | TCATTTTGATTCATTCATTC+TGG | + | chr3.3:68799786-68799805 | MS.gene044282:intron | 25.0% |
| AGTATAAGAATGAGGCATAT+AGG | + | chr3.3:68799651-68799670 | MS.gene044282:CDS | 30.0% | |
| CCGTGAAAAAAACAATAATC+AGG | + | chr3.3:68799956-68799975 | MS.gene044282:CDS | 30.0% | |
| GGCTCAAATTCAAATCTATA+CGG | + | chr3.3:68799682-68799701 | MS.gene044282:CDS | 30.0% | |
| AACATCATCGGATATGTGAA+GGG | - | chr3.3:68799892-68799911 | None:intergenic | 35.0% | |
| ATTAAATGTGATCTTGGTGG+AGG | - | chr3.3:68799849-68799868 | None:intergenic | 35.0% | |
| CTTGGAAAGTTGTTAAAGCA+GGG | - | chr3.3:68800198-68800217 | None:intergenic | 35.0% | |
| GAACAGAAACAGGTGAAAAA+GGG | + | chr3.3:68799530-68799549 | MS.gene044282:CDS | 35.0% | |
| GATTCCGACTTTCCTTATTA+CGG | + | chr3.3:68799611-68799630 | MS.gene044282:CDS | 35.0% | |
| TACGTCACAAGATCTTCAAT+GGG | - | chr3.3:68799581-68799600 | None:intergenic | 35.0% | |
| TTAAATGTGATCTTGGTGGA+GGG | - | chr3.3:68799848-68799867 | None:intergenic | 35.0% | |
| TTCTACCATTAATAAGGGCA+TGG | - | chr3.3:68800123-68800142 | None:intergenic | 35.0% | |
| TTGGAAAGTTGTTAAAGCAG+GGG | - | chr3.3:68800197-68800216 | None:intergenic | 35.0% | |
| TTTGCCGTAATAAGGAAAGT+CGG | - | chr3.3:68799618-68799637 | None:intergenic | 35.0% | |
| ! | ATCTTCTTCTTCAGCTTTTG+CGG | - | chr3.3:68800164-68800183 | None:intergenic | 35.0% |
| ! | TCTACCATTAATAAGGGCAT+GGG | - | chr3.3:68800122-68800141 | None:intergenic | 35.0% |
| AAACAGGTGAAAAAGGGATC+GGG | + | chr3.3:68799536-68799555 | MS.gene044282:CDS | 40.0% | |
| ACATCATCGGATATGTGAAG+GGG | - | chr3.3:68799891-68799910 | None:intergenic | 40.0% | |
| GAAACAGGTGAAAAAGGGAT+CGG | + | chr3.3:68799535-68799554 | MS.gene044282:CDS | 40.0% | |
| GAACATCATCGGATATGTGA+AGG | - | chr3.3:68799893-68799912 | None:intergenic | 40.0% | |
| GCTTGGAAAGTTGTTAAAGC+AGG | - | chr3.3:68800199-68800218 | None:intergenic | 40.0% | |
| GGAACAGAAACAGGTGAAAA+AGG | + | chr3.3:68799529-68799548 | MS.gene044282:CDS | 40.0% | |
| GTAATAGTCCAATGCAAGCT+TGG | - | chr3.3:68799937-68799956 | None:intergenic | 40.0% | |
| GTACGTCACAAGATCTTCAA+TGG | - | chr3.3:68799582-68799601 | None:intergenic | 40.0% | |
| TAAATGTGATCTTGGTGGAG+GGG | - | chr3.3:68799847-68799866 | None:intergenic | 40.0% | |
| TCTGATCTCAGGTGGTTTAA+TGG | - | chr3.3:68800269-68800288 | None:intergenic | 40.0% | |
| TGTACCCATGCCCTTATTAA+TGG | + | chr3.3:68800115-68800134 | MS.gene044282:CDS | 40.0% | |
| TTACCACGTTTGCCGTAATA+AGG | - | chr3.3:68799626-68799645 | None:intergenic | 40.0% | |
| TTTCCTTATTACGGCAAACG+TGG | + | chr3.3:68799620-68799639 | MS.gene044282:CDS | 40.0% | |
| ! | TTCTTCAGCTTTTGCGGTAA+AGG | - | chr3.3:68800158-68800177 | None:intergenic | 40.0% |
| AGGCCATGAAACACAAAAGC+TGG | - | chr3.3:68800088-68800107 | None:intergenic | 45.0% | |
| GAACACAGCATACTTCAGCT+TGG | - | chr3.3:68800216-68800235 | None:intergenic | 45.0% | |
| GAGAATAGGGCGAACATCAT+CGG | - | chr3.3:68799904-68799923 | None:intergenic | 45.0% | |
| TGAGGCATATAGGATGCTTG+AGG | + | chr3.3:68799661-68799680 | MS.gene044282:CDS | 45.0% | |
| TGGACAACACAACAAAACGC+AGG | + | chr3.3:68799806-68799825 | MS.gene044282:intron | 45.0% | |
| TGTTCGAGACAAACAACCTG+TGG | + | chr3.3:68800231-68800250 | MS.gene044282:CDS | 45.0% | |
| ! | AGTTGTTAAAGCAGGGGTGT+CGG | - | chr3.3:68800191-68800210 | None:intergenic | 45.0% |
| ! | GGTCCAGCTTTTGTGTTTCA+TGG | + | chr3.3:68800082-68800101 | MS.gene044282:CDS | 45.0% |
| ! | TGGAGTGTCCTTCGAGAATA+GGG | - | chr3.3:68799917-68799936 | None:intergenic | 45.0% |
| ! | TTGGAGTGTCCTTCGAGAAT+AGG | - | chr3.3:68799918-68799937 | None:intergenic | 45.0% |
| AGGACACTCCAAGCTTGCAT+TGG | + | chr3.3:68799926-68799945 | MS.gene044282:CDS | 50.0% | |
| ATGGCACACTCTTTGACCAC+AGG | - | chr3.3:68800250-68800269 | None:intergenic | 50.0% | |
| GATGTTCGCCCTATTCTCGA+AGG | + | chr3.3:68799906-68799925 | MS.gene044282:CDS | 50.0% | |
| GGTGAAAAAGGGATCGGGAT+CGG | + | chr3.3:68799541-68799560 | MS.gene044282:CDS | 50.0% | |
| GTGAAAAAGGGATCGGGATC+GGG | + | chr3.3:68799542-68799561 | MS.gene044282:CDS | 50.0% | |
| AAAGGGATCGGGATCGGGAT+TGG | + | chr3.3:68799547-68799566 | MS.gene044282:CDS | 55.0% | |
| GCACACTCTCTGATCTCAGG+TGG | - | chr3.3:68800277-68800296 | None:intergenic | 55.0% | |
| GGGCATGGGTACACTTGACA+AGG | - | chr3.3:68800108-68800127 | None:intergenic | 55.0% | |
| GTGGCACACTCTCTGATCTC+AGG | - | chr3.3:68800280-68800299 | None:intergenic | 55.0% | |
| TGATCTTGGTGGAGGGGCGA+TGG | - | chr3.3:68799841-68799860 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 68799527 | 68800309 | 68799527 | ID=MS.gene044282 |
| chr3.3 | mRNA | 68799527 | 68800309 | 68799527 | ID=MS.gene044282.t1;Parent=MS.gene044282 |
| chr3.3 | exon | 68799527 | 68799724 | 68799527 | ID=MS.gene044282.t1.exon1;Parent=MS.gene044282.t1 |
| chr3.3 | CDS | 68799527 | 68799724 | 68799527 | ID=cds.MS.gene044282.t1;Parent=MS.gene044282.t1 |
| chr3.3 | exon | 68799828 | 68799977 | 68799828 | ID=MS.gene044282.t1.exon2;Parent=MS.gene044282.t1 |
| chr3.3 | CDS | 68799828 | 68799977 | 68799828 | ID=cds.MS.gene044282.t1;Parent=MS.gene044282.t1 |
| chr3.3 | exon | 68800082 | 68800309 | 68800082 | ID=MS.gene044282.t1.exon3;Parent=MS.gene044282.t1 |
| chr3.3 | CDS | 68800082 | 68800309 | 68800082 | ID=cds.MS.gene044282.t1;Parent=MS.gene044282.t1 |
| Gene Sequence |
| Protein sequence |
