Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044364.t1 | AES71816.1 | 60.1 | 238 | 62 | 3 | 1 | 238 | 1 | 205 | 1.60E-69 | 272.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044364.t1 | G7J9H7 | 60.1 | 238 | 62 | 3 | 1 | 238 | 1 | 205 | 1.2e-69 | 272.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044364 | MS.gene049263 | PPI |
| MS.gene77472 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene71531 | PPI |
| MS.gene77468 | MS.gene044364 | PPI |
| MS.gene25704 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene044323 | PPI |
| MS.gene044361 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene045214 | PPI |
| MS.gene79115 | MS.gene044364 | PPI |
| MS.gene044370 | MS.gene044364 | PPI |
| MS.gene70476 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene71282 | PPI |
| MS.gene70443 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene71524 | PPI |
| MS.gene044364 | MS.gene71528 | PPI |
| MS.gene044364 | MS.gene70424 | PPI |
| MS.gene044376 | MS.gene044364 | PPI |
| MS.gene044363 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene77464 | PPI |
| MS.gene70430 | MS.gene044364 | PPI |
| MS.gene77466 | MS.gene044364 | PPI |
| MS.gene70418 | MS.gene044364 | PPI |
| MS.gene044364 | MS.gene044326 | PPI |
| MS.gene044364 | MS.gene70427 | PPI |
| MS.gene71284 | MS.gene044364 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044364.t1 | MTR_3g083030 | 66.250 | 240 | 70 | 3 | 1 | 239 | 1 | 230 | 1.29e-104 | 302 |
| MS.gene044364.t1 | MTR_3g083080 | 62.185 | 238 | 57 | 3 | 1 | 238 | 1 | 205 | 3.20e-100 | 290 |
| MS.gene044364.t1 | MTR_3g083050 | 53.333 | 240 | 78 | 3 | 1 | 239 | 1 | 207 | 8.78e-76 | 228 |
| MS.gene044364.t1 | MTR_3g082340 | 35.628 | 247 | 125 | 8 | 1 | 238 | 1 | 222 | 6.55e-36 | 127 |
| MS.gene044364.t1 | MTR_3g082420 | 38.696 | 230 | 118 | 9 | 17 | 238 | 7 | 221 | 7.89e-35 | 124 |
| MS.gene044364.t1 | MTR_3g086030 | 51.852 | 108 | 44 | 1 | 54 | 161 | 4 | 103 | 3.99e-28 | 104 |
| MS.gene044364.t1 | MTR_3g083150 | 36.327 | 245 | 117 | 8 | 1 | 239 | 1 | 212 | 1.96e-25 | 99.8 |
| MS.gene044364.t1 | MTR_3g080680 | 38.942 | 208 | 104 | 10 | 39 | 238 | 6 | 198 | 8.29e-22 | 89.7 |
| MS.gene044364.t1 | MTR_3g083100 | 42.063 | 126 | 59 | 3 | 128 | 239 | 49 | 174 | 3.96e-20 | 84.7 |
| MS.gene044364.t1 | MTR_8g461300 | 38.728 | 173 | 81 | 10 | 83 | 238 | 34 | 198 | 1.13e-18 | 81.3 |
| MS.gene044364.t1 | MTR_4g055800 | 30.345 | 145 | 79 | 5 | 86 | 222 | 143 | 273 | 1.18e-13 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 61 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGAGGGGAGTGAGTTTA+AGG | 0.216980 | 3.3:-69782299 | None:intergenic |
| TTCAGTTCCGGTGGCAATTC+CGG | 0.272860 | 3.3:-69781871 | None:intergenic |
| TCGCCAGAGCTAAGCATAAA+AGG | 0.284068 | 3.3:+69782094 | MS.gene044364:CDS |
| TCCGGTTCCGACTTCAGTTC+GGG | 0.301504 | 3.3:-69781853 | None:intergenic |
| TCGGGTTCAGGTTGCAATTC+CGG | 0.306399 | 3.3:-69781835 | None:intergenic |
| TCATATTTCTGTCTCTGTTT+CGG | 0.331334 | 3.3:-69781931 | None:intergenic |
| TCCGACTTCAGTTCGGGTTC+AGG | 0.333407 | 3.3:-69781847 | None:intergenic |
| TTCGGTTGCGACTTCAGTTC+CGG | 0.367780 | 3.3:-69781913 | None:intergenic |
| TCCGGTTCCAACTTCAGTTC+CGG | 0.379777 | 3.3:-69781883 | None:intergenic |
| TTCCGGTTCCGACTTCAGTT+CGG | 0.385258 | 3.3:-69781854 | None:intergenic |
| GCGATTTACGTATAAGAATA+AGG | 0.390721 | 3.3:+69782041 | MS.gene044364:CDS |
| GCAATACAATGCTAAGAATC+AGG | 0.399334 | 3.3:+69782335 | MS.gene044364:CDS |
| AAGGCGAAGATCATCGGTTA+TGG | 0.404672 | 3.3:-69782280 | None:intergenic |
| TCTTGTTCCATACGTGGGCC+TGG | 0.449501 | 3.3:-69782569 | None:intergenic |
| TTCAGGTTGCAATTCCGGTT+CGG | 0.449691 | 3.3:-69781830 | None:intergenic |
| GAATCACATACATGTTTGTT+CGG | 0.450921 | 3.3:+69781754 | MS.gene044364:CDS |
| CACGTATGGAACAAGAGGCC+TGG | 0.458410 | 3.3:+69782576 | MS.gene044364:CDS |
| ACCCGAACTGAAGTCGGAAC+CGG | 0.459886 | 3.3:+69781852 | MS.gene044364:CDS |
| CGCACACACTTAAACACAAA+AGG | 0.462554 | 3.3:-69782021 | None:intergenic |
| TCCCCTCTGCAAGCTTGCGT+TGG | 0.466151 | 3.3:+69782311 | MS.gene044364:CDS |
| AACAGAGACAGAAATATGAA+TGG | 0.466188 | 3.3:+69781935 | MS.gene044364:CDS |
| TTCAGTTCCGGTGCTAACTC+CGG | 0.468654 | 3.3:-69781901 | None:intergenic |
| TGGGCCTGGAAAATTATAAC+AGG | 0.482469 | 3.3:-69782555 | None:intergenic |
| ACGTATGGAACAAGAGGCCT+GGG | 0.488378 | 3.3:+69782577 | MS.gene044364:CDS |
| ACCGGAACTGAAGTTGGAAC+CGG | 0.489325 | 3.3:+69781882 | MS.gene044364:CDS |
| GACACCACCTCAGGTGGCCC+AGG | 0.497237 | 3.3:-69782594 | None:intergenic |
| ATATATACCACCAGGTCTCC+AGG | 0.503658 | 3.3:-69782481 | None:intergenic |
| TGGAGGAGTAATAGCATCAA+AGG | 0.514021 | 3.3:-69782220 | None:intergenic |
| AGAAAAGCAGAAATATGTTA+CGG | 0.518698 | 3.3:+69782243 | MS.gene044364:CDS |
| ATTGCCACCGGAACTGAAGT+TGG | 0.518984 | 3.3:+69781876 | MS.gene044364:CDS |
| CAGGCCTCTTGTTCCATACG+TGG | 0.522394 | 3.3:-69782575 | None:intergenic |
| GAGTTTAAGGCGAAGATCAT+CGG | 0.527340 | 3.3:-69782286 | None:intergenic |
| ACAGAGACAGAAATATGAAT+GGG | 0.527605 | 3.3:+69781936 | MS.gene044364:CDS |
| GAGGCCTGGGCCACCTGAGG+TGG | 0.535128 | 3.3:+69782590 | MS.gene044364:CDS |
| GAAAATTATAACAGGTCTGT+CGG | 0.536484 | 3.3:-69782547 | None:intergenic |
| CTCCAACGCAAGCTTGCAGA+GGG | 0.537666 | 3.3:-69782313 | None:intergenic |
| ATGGCACACGACACCACCTC+AGG | 0.542265 | 3.3:-69782603 | None:intergenic |
| CTGACATTGTGAAGACAACC+TGG | 0.546641 | 3.3:+69782463 | MS.gene044364:CDS |
| AGATCATCGGTTATGGAAAG+TGG | 0.556078 | 3.3:-69782273 | None:intergenic |
| AGGCCTCTTGTTCCATACGT+GGG | 0.562166 | 3.3:-69782574 | None:intergenic |
| GCGGATAGCTGTGATGAAAG+AGG | 0.565108 | 3.3:+69781793 | MS.gene044364:CDS |
| GAAGTGGCTGAAATCCGAAC+CGG | 0.569273 | 3.3:+69781816 | MS.gene044364:CDS |
| CGGATAGCTGTGATGAAAGA+GGG | 0.570369 | 3.3:+69781794 | MS.gene044364:CDS |
| CACAGCTATCCGCAGAAGGT+CGG | 0.575053 | 3.3:-69781783 | None:intergenic |
| ACCTGAACCCGAACTGAAGT+CGG | 0.580010 | 3.3:+69781846 | MS.gene044364:CDS |
| CAAGAGGCCTGGGCCACCTG+AGG | 0.581372 | 3.3:+69782587 | MS.gene044364:CDS |
| GGAGGAGTAATAGCATCAAA+GGG | 0.583487 | 3.3:-69782219 | None:intergenic |
| CGGCGTCATCCGACCTTCTG+CGG | 0.584101 | 3.3:+69781774 | MS.gene044364:CDS |
| ATTATAACAGGTCTGTCGGA+AGG | 0.593654 | 3.3:-69782543 | None:intergenic |
| GTCGGAACCGGAATTGCCAC+CGG | 0.601982 | 3.3:+69781864 | MS.gene044364:CDS |
| GTGAAGACAACCTGGAGACC+TGG | 0.604519 | 3.3:+69782471 | MS.gene044364:CDS |
| TCATCACAGCTATCCGCAGA+AGG | 0.611091 | 3.3:-69781787 | None:intergenic |
| GTTGGAACCGGAGTTAGCAC+CGG | 0.614360 | 3.3:+69781894 | MS.gene044364:CDS |
| GGTTCCAACTTCAGTTCCGG+TGG | 0.621297 | 3.3:-69781880 | None:intergenic |
| AGGCCCACGTATGGAACAAG+AGG | 0.623282 | 3.3:+69782571 | MS.gene044364:CDS |
| GCACACGACACCACCTCAGG+TGG | 0.634060 | 3.3:-69782600 | None:intergenic |
| GCTCCAACGCAAGCTTGCAG+AGG | 0.640098 | 3.3:-69782314 | None:intergenic |
| GTGATGTAATATATACCACC+AGG | 0.685832 | 3.3:-69782489 | None:intergenic |
| GCTGTGATGAAAGAGGGAAG+TGG | 0.711241 | 3.3:+69781800 | MS.gene044364:CDS |
| TCCAACGCAAGCTTGCAGAG+GGG | 0.750745 | 3.3:-69782312 | None:intergenic |
| AAGACAACCTGGAGACCTGG+TGG | 0.761347 | 3.3:+69782474 | MS.gene044364:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTATGCTTCTTAATTATTA+GGG | + | chr3.3:69782423-69782442 | MS.gene044364:intron | 20.0% |
| !! | TAGTATGCTTCTTAATTATT+AGG | + | chr3.3:69782422-69782441 | MS.gene044364:intron | 20.0% |
| ! | AGAAAAGCAGAAATATGTTA+CGG | + | chr3.3:69782243-69782262 | MS.gene044364:CDS | 25.0% |
| !!! | TAACATATTTCTGCTTTTCT+TGG | - | chr3.3:69782243-69782262 | None:intergenic | 25.0% |
| AACAGAGACAGAAATATGAA+TGG | + | chr3.3:69781935-69781954 | MS.gene044364:CDS | 30.0% | |
| ACAGAGACAGAAATATGAAT+GGG | + | chr3.3:69781936-69781955 | MS.gene044364:CDS | 30.0% | |
| GAAAATTATAACAGGTCTGT+CGG | - | chr3.3:69782550-69782569 | None:intergenic | 30.0% | |
| GAATCACATACATGTTTGTT+CGG | + | chr3.3:69781754-69781773 | MS.gene044364:CDS | 30.0% | |
| GCGATTTACGTATAAGAATA+AGG | + | chr3.3:69782041-69782060 | MS.gene044364:CDS | 30.0% | |
| GGCTCAGATTAAAAAAGATA+AGG | + | chr3.3:69782062-69782081 | MS.gene044364:CDS | 30.0% | |
| ! | TCATATTTCTGTCTCTGTTT+CGG | - | chr3.3:69781934-69781953 | None:intergenic | 30.0% |
| !!! | TTCTTGTTTTTGTGCTTGAA+AGG | - | chr3.3:69782514-69782533 | None:intergenic | 30.0% |
| GCAATACAATGCTAAGAATC+AGG | + | chr3.3:69782335-69782354 | MS.gene044364:CDS | 35.0% | |
| GTGATGTAATATATACCACC+AGG | - | chr3.3:69782492-69782511 | None:intergenic | 35.0% | |
| ! | CAGACCTGTTATAATTTTCC+AGG | + | chr3.3:69782551-69782570 | MS.gene044364:CDS | 35.0% |
| !! | CATATTTCTGCTTTTCTTGG+AGG | - | chr3.3:69782240-69782259 | None:intergenic | 35.0% |
| ATTATAACAGGTCTGTCGGA+AGG | - | chr3.3:69782546-69782565 | None:intergenic | 40.0% | |
| CGCACACACTTAAACACAAA+AGG | - | chr3.3:69782024-69782043 | None:intergenic | 40.0% | |
| GAGATAGATGCGTTACAGAT+AGG | - | chr3.3:69782131-69782150 | None:intergenic | 40.0% | |
| GGAGGAGTAATAGCATCAAA+GGG | - | chr3.3:69782222-69782241 | None:intergenic | 40.0% | |
| TGGAGGAGTAATAGCATCAA+AGG | - | chr3.3:69782223-69782242 | None:intergenic | 40.0% | |
| TGGGCCTGGAAAATTATAAC+AGG | - | chr3.3:69782558-69782577 | None:intergenic | 40.0% | |
| ! | AGATCATCGGTTATGGAAAG+TGG | - | chr3.3:69782276-69782295 | None:intergenic | 40.0% |
| ! | GAGTTTAAGGCGAAGATCAT+CGG | - | chr3.3:69782289-69782308 | None:intergenic | 40.0% |
| ATATATACCACCAGGTCTCC+AGG | - | chr3.3:69782484-69782503 | None:intergenic | 45.0% | |
| CGGATAGCTGTGATGAAAGA+GGG | + | chr3.3:69781794-69781813 | MS.gene044364:CDS | 45.0% | |
| CTGACATTGTGAAGACAACC+TGG | + | chr3.3:69782463-69782482 | MS.gene044364:CDS | 45.0% | |
| TCGCCAGAGCTAAGCATAAA+AGG | + | chr3.3:69782094-69782113 | MS.gene044364:CDS | 45.0% | |
| ! | AAGGCGAAGATCATCGGTTA+TGG | - | chr3.3:69782283-69782302 | None:intergenic | 45.0% |
| ! | ACGCCTTTTATGCTTAGCTC+TGG | - | chr3.3:69782100-69782119 | None:intergenic | 45.0% |
| ! | TAATTTTCCAGGCCCACGTA+TGG | + | chr3.3:69782562-69782581 | MS.gene044364:CDS | 45.0% |
| !! | TTCAGGTTGCAATTCCGGTT+CGG | - | chr3.3:69781833-69781852 | None:intergenic | 45.0% |
| !!! | TTAATTTAATTTCTTAATTT+TGG | + | chr3.3:69782181-69782200 | MS.gene044364:intron | 5.0% |
| ACCGGAACTGAAGTTGGAAC+CGG | + | chr3.3:69781882-69781901 | MS.gene044364:CDS | 50.0% | |
| ACCTGAACCCGAACTGAAGT+CGG | + | chr3.3:69781846-69781865 | MS.gene044364:CDS | 50.0% | |
| ACGTATGGAACAAGAGGCCT+GGG | + | chr3.3:69782577-69782596 | MS.gene044364:CDS | 50.0% | |
| AGGCCTCTTGTTCCATACGT+GGG | - | chr3.3:69782577-69782596 | None:intergenic | 50.0% | |
| ATTGCCACCGGAACTGAAGT+TGG | + | chr3.3:69781876-69781895 | MS.gene044364:CDS | 50.0% | |
| GAAGTGGCTGAAATCCGAAC+CGG | + | chr3.3:69781816-69781835 | MS.gene044364:CDS | 50.0% | |
| GCGGATAGCTGTGATGAAAG+AGG | + | chr3.3:69781793-69781812 | MS.gene044364:CDS | 50.0% | |
| GCTGTGATGAAAGAGGGAAG+TGG | + | chr3.3:69781800-69781819 | MS.gene044364:CDS | 50.0% | |
| TCATCACAGCTATCCGCAGA+AGG | - | chr3.3:69781790-69781809 | None:intergenic | 50.0% | |
| TCCGGTTCCAACTTCAGTTC+CGG | - | chr3.3:69781886-69781905 | None:intergenic | 50.0% | |
| TGCAGAGGGGAGTGAGTTTA+AGG | - | chr3.3:69782302-69782321 | None:intergenic | 50.0% | |
| TTCAGTTCCGGTGGCAATTC+CGG | - | chr3.3:69781874-69781893 | None:intergenic | 50.0% | |
| TTCCGGTTCCGACTTCAGTT+CGG | - | chr3.3:69781857-69781876 | None:intergenic | 50.0% | |
| TTCGGTTGCGACTTCAGTTC+CGG | - | chr3.3:69781916-69781935 | None:intergenic | 50.0% | |
| ! | TTCAGTTCCGGTGCTAACTC+CGG | - | chr3.3:69781904-69781923 | None:intergenic | 50.0% |
| !! | TCGGGTTCAGGTTGCAATTC+CGG | - | chr3.3:69781838-69781857 | None:intergenic | 50.0% |
| AAGACAACCTGGAGACCTGG+TGG | + | chr3.3:69782474-69782493 | MS.gene044364:CDS | 55.0% | |
| ACCCGAACTGAAGTCGGAAC+CGG | + | chr3.3:69781852-69781871 | MS.gene044364:CDS | 55.0% | |
| AGGCCCACGTATGGAACAAG+AGG | + | chr3.3:69782571-69782590 | MS.gene044364:CDS | 55.0% | |
| CACAGCTATCCGCAGAAGGT+CGG | - | chr3.3:69781786-69781805 | None:intergenic | 55.0% | |
| CACGTATGGAACAAGAGGCC+TGG | + | chr3.3:69782576-69782595 | MS.gene044364:CDS | 55.0% | |
| CAGGCCTCTTGTTCCATACG+TGG | - | chr3.3:69782578-69782597 | None:intergenic | 55.0% | |
| CTCCAACGCAAGCTTGCAGA+GGG | - | chr3.3:69782316-69782335 | None:intergenic | 55.0% | |
| GGTTCCAACTTCAGTTCCGG+TGG | - | chr3.3:69781883-69781902 | None:intergenic | 55.0% | |
| GTGAAGACAACCTGGAGACC+TGG | + | chr3.3:69782471-69782490 | MS.gene044364:CDS | 55.0% | |
| GTTGGAACCGGAGTTAGCAC+CGG | + | chr3.3:69781894-69781913 | MS.gene044364:CDS | 55.0% | |
| TCCAACGCAAGCTTGCAGAG+GGG | - | chr3.3:69782315-69782334 | None:intergenic | 55.0% | |
| TCCGGTTCCGACTTCAGTTC+GGG | - | chr3.3:69781856-69781875 | None:intergenic | 55.0% | |
| TCTTGTTCCATACGTGGGCC+TGG | - | chr3.3:69782572-69782591 | None:intergenic | 55.0% | |
| ! | TCCGACTTCAGTTCGGGTTC+AGG | - | chr3.3:69781850-69781869 | None:intergenic | 55.0% |
| ATGGCACACGACACCACCTC+AGG | - | chr3.3:69782606-69782625 | None:intergenic | 60.0% | |
| GCTCCAACGCAAGCTTGCAG+AGG | - | chr3.3:69782317-69782336 | None:intergenic | 60.0% | |
| GTCGGAACCGGAATTGCCAC+CGG | + | chr3.3:69781864-69781883 | MS.gene044364:CDS | 60.0% | |
| TCCCCTCTGCAAGCTTGCGT+TGG | + | chr3.3:69782311-69782330 | MS.gene044364:CDS | 60.0% | |
| CGGCGTCATCCGACCTTCTG+CGG | + | chr3.3:69781774-69781793 | MS.gene044364:CDS | 65.0% | |
| GCACACGACACCACCTCAGG+TGG | - | chr3.3:69782603-69782622 | None:intergenic | 65.0% | |
| CAAGAGGCCTGGGCCACCTG+AGG | + | chr3.3:69782587-69782606 | MS.gene044364:CDS | 70.0% | |
| GACACCACCTCAGGTGGCCC+AGG | - | chr3.3:69782597-69782616 | None:intergenic | 70.0% | |
| GAGGCCTGGGCCACCTGAGG+TGG | + | chr3.3:69782590-69782609 | MS.gene044364:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69781745 | 69782635 | 69781745 | ID=MS.gene044364 |
| chr3.3 | mRNA | 69781745 | 69782635 | 69781745 | ID=MS.gene044364.t1;Parent=MS.gene044364 |
| chr3.3 | exon | 69781745 | 69782134 | 69781745 | ID=MS.gene044364.t1.exon1;Parent=MS.gene044364.t1 |
| chr3.3 | CDS | 69781745 | 69782134 | 69781745 | ID=cds.MS.gene044364.t1;Parent=MS.gene044364.t1 |
| chr3.3 | exon | 69782219 | 69782356 | 69782219 | ID=MS.gene044364.t1.exon2;Parent=MS.gene044364.t1 |
| chr3.3 | CDS | 69782219 | 69782356 | 69782219 | ID=cds.MS.gene044364.t1;Parent=MS.gene044364.t1 |
| chr3.3 | exon | 69782444 | 69782635 | 69782444 | ID=MS.gene044364.t1.exon3;Parent=MS.gene044364.t1 |
| chr3.3 | CDS | 69782444 | 69782635 | 69782444 | ID=cds.MS.gene044364.t1;Parent=MS.gene044364.t1 |
| Gene Sequence |
| Protein sequence |
