Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044369.t1 | GAU15170.1 | 48.9 | 135 | 66 | 2 | 13 | 144 | 37 | 171 | 2.50E-25 | 125.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044369.t1 | A0A392P375 | 51.5 | 130 | 60 | 2 | 18 | 144 | 40 | 169 | 4.8e-26 | 127.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044376 | MS.gene044369 | PPI |
| MS.gene044363 | MS.gene044369 | PPI |
| MS.gene71284 | MS.gene044369 | PPI |
| MS.gene77472 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene044323 | PPI |
| MS.gene70418 | MS.gene044369 | PPI |
| MS.gene77466 | MS.gene044369 | PPI |
| MS.gene77468 | MS.gene044369 | PPI |
| MS.gene25704 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene71531 | PPI |
| MS.gene044369 | MS.gene71282 | PPI |
| MS.gene044361 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene71524 | PPI |
| MS.gene044369 | MS.gene71528 | PPI |
| MS.gene044370 | MS.gene044369 | PPI |
| MS.gene70476 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene045214 | PPI |
| MS.gene044369 | MS.gene77464 | PPI |
| MS.gene70443 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene044326 | PPI |
| MS.gene79115 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene70427 | PPI |
| MS.gene044369 | MS.gene70424 | PPI |
| MS.gene70430 | MS.gene044369 | PPI |
| MS.gene044369 | MS.gene049263 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044369.t1 | MTR_3g083080 | 44.898 | 147 | 73 | 5 | 1 | 144 | 66 | 207 | 6.07e-30 | 107 |
| MS.gene044369.t1 | MTR_3g083050 | 37.415 | 147 | 83 | 6 | 1 | 144 | 68 | 208 | 2.57e-18 | 77.8 |
| MS.gene044369.t1 | MTR_3g083030 | 38.462 | 143 | 79 | 6 | 5 | 144 | 95 | 231 | 4.00e-18 | 77.8 |
| MS.gene044369.t1 | MTR_3g083100 | 43.956 | 91 | 43 | 3 | 60 | 142 | 83 | 173 | 2.30e-15 | 69.3 |
| MS.gene044369.t1 | MTR_3g082420 | 39.167 | 120 | 61 | 6 | 33 | 142 | 104 | 221 | 5.15e-13 | 63.9 |
| MS.gene044369.t1 | MTR_3g082340 | 39.167 | 120 | 61 | 6 | 33 | 142 | 105 | 222 | 5.38e-13 | 63.9 |
| MS.gene044369.t1 | MTR_3g083150 | 38.793 | 116 | 56 | 5 | 37 | 142 | 101 | 211 | 8.10e-12 | 60.5 |
| MS.gene044369.t1 | MTR_3g080680 | 40.870 | 115 | 56 | 6 | 37 | 142 | 87 | 198 | 9.32e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACATCATCAGTTATGTAAA+TGG | 0.241862 | 3.3:-69822874 | None:intergenic |
| CTCTTTCCTCACAGCTTTCC+CGG | 0.295531 | 3.3:-69822414 | None:intergenic |
| CGGAATTCCATCAAAGATTT+CGG | 0.332800 | 3.3:-69823163 | None:intergenic |
| CAGTCCTGCTATAACTTTCC+AGG | 0.342416 | 3.3:+69823140 | MS.gene044369:CDS |
| TTCCAGGCCGAAATCTTTGA+TGG | 0.364306 | 3.3:+69823156 | MS.gene044369:CDS |
| GTACTTGTCTAACGCAATCT+TGG | 0.389784 | 3.3:-69822919 | None:intergenic |
| CGGCATCACCCAACCTTACC+CGG | 0.405800 | 3.3:+69822395 | MS.gene044369:CDS |
| CCTCACAGCTTTCCCGGGTA+AGG | 0.423425 | 3.3:-69822408 | None:intergenic |
| CAGCTTTCCCGGGTAAGGTT+GGG | 0.426504 | 3.3:-69822403 | None:intergenic |
| TTGTGTTTGATGACCTTGTC+AGG | 0.426633 | 3.3:+69823043 | MS.gene044369:CDS |
| ACATCATCAGTTATGTAAAT+GGG | 0.440245 | 3.3:-69822873 | None:intergenic |
| TAGCTTGAAAGGTAATGTAA+TGG | 0.463220 | 3.3:-69823095 | None:intergenic |
| ACAGCTTTCCCGGGTAAGGT+TGG | 0.466038 | 3.3:-69822404 | None:intergenic |
| AGACAAGTACAATGCTGATC+AGG | 0.474826 | 3.3:+69822932 | MS.gene044369:CDS |
| TCAACCCGTCAATGCGTTCA+AGG | 0.482744 | 3.3:+69823066 | MS.gene044369:CDS |
| TATATGTTAGGTAGGCTTCA+TGG | 0.483461 | 3.3:-69823217 | None:intergenic |
| TTTACTGATTCATAACCAAA+AGG | 0.490944 | 3.3:-69822236 | None:intergenic |
| CCTTGGTGTATATGTTAGGT+AGG | 0.492491 | 3.3:-69823225 | None:intergenic |
| AAAACCTTGAACGCATTGAC+GGG | 0.497313 | 3.3:-69823070 | None:intergenic |
| TGTAGCATCTTTAGCTTGAA+AGG | 0.500229 | 3.3:-69823106 | None:intergenic |
| TTGGTTATGAATCAGTAAAG+AGG | 0.501426 | 3.3:+69822240 | MS.gene044369:CDS |
| GAAAGTTATAGCAGGACTGC+TGG | 0.513254 | 3.3:-69823136 | None:intergenic |
| TAAAACCTTGAACGCATTGA+CGG | 0.514956 | 3.3:-69823071 | None:intergenic |
| TCTTTCCTCACAGCTTTCCC+GGG | 0.516141 | 3.3:-69822413 | None:intergenic |
| TTTGATGGAATTCCGTCAGA+TGG | 0.523631 | 3.3:+69823171 | MS.gene044369:CDS |
| TTGGGTGATGCCGTGCAAGC+AGG | 0.523717 | 3.3:-69822385 | None:intergenic |
| TTCCATCAAAGATTTCGGCC+TGG | 0.534165 | 3.3:-69823158 | None:intergenic |
| TATGAATCAGTAAAGAGGAT+TGG | 0.540096 | 3.3:+69822245 | MS.gene044369:CDS |
| GAGTCGGATACCTGCTTGCA+CGG | 0.558901 | 3.3:+69822375 | MS.gene044369:CDS |
| GTCTAACGCAATCTTGGAGA+AGG | 0.569250 | 3.3:-69822913 | None:intergenic |
| GACCCTCCACAAGGTTTATG+TGG | 0.573237 | 3.3:+69822840 | MS.gene044369:CDS |
| TCGGCCTGGAAAGTTATAGC+AGG | 0.573488 | 3.3:-69823144 | None:intergenic |
| GGCATCACCCAACCTTACCC+GGG | 0.579942 | 3.3:+69822396 | MS.gene044369:CDS |
| GACGCTATCGACCCTCCACA+AGG | 0.594289 | 3.3:+69822831 | MS.gene044369:CDS |
| ACCACAGGCGGTCCATCTGA+CGG | 0.602174 | 3.3:-69823183 | None:intergenic |
| GATCAGAATGTCGATGGAGT+CGG | 0.604517 | 3.3:+69822359 | MS.gene044369:intron |
| TCCGTCAGATGGACCGCCTG+TGG | 0.607223 | 3.3:+69823182 | MS.gene044369:CDS |
| GTTACAGATCAGAATGTCGA+TGG | 0.612079 | 3.3:+69822353 | MS.gene044369:intron |
| GTTATAGCAGGACTGCTGGA+AGG | 0.641064 | 3.3:-69823132 | None:intergenic |
| CGCCACATAAACCTTGTGGA+GGG | 0.644987 | 3.3:-69822842 | None:intergenic |
| CCGCCACATAAACCTTGTGG+AGG | 0.646649 | 3.3:-69822843 | None:intergenic |
| ATTGACGGGTTGACCTGACA+AGG | 0.647456 | 3.3:-69823056 | None:intergenic |
| ATGGCACACGAACTAACCAC+AGG | 0.663365 | 3.3:-69823198 | None:intergenic |
| ATACCGCCACATAAACCTTG+TGG | 0.663420 | 3.3:-69822846 | None:intergenic |
| TGGAGAAGGTAATGAGACGA+GGG | 0.666829 | 3.3:-69822899 | None:intergenic |
| TTGGAGAAGGTAATGAGACG+AGG | 0.668902 | 3.3:-69822900 | None:intergenic |
| CCTCCACAAGGTTTATGTGG+CGG | 0.672471 | 3.3:+69822843 | MS.gene044369:CDS |
| GCACACGAACTAACCACAGG+CGG | 0.703587 | 3.3:-69823195 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTCTTCTTAATTATT+AGG | + | chr3.3:69823011-69823030 | MS.gene044369:intron | 10.0% |
| !! | ATTATTCTTCTTAATTATTA+GGG | + | chr3.3:69823012-69823031 | MS.gene044369:intron | 10.0% |
| !! | AATATATCAAGTGAAACAAA+GGG | - | chr3.3:69822779-69822798 | None:intergenic | 20.0% |
| !! | ATATTATCTTAATCTCATTG+TGG | + | chr3.3:69822794-69822813 | MS.gene044369:intron | 20.0% |
| !! | TAATATATCAAGTGAAACAA+AGG | - | chr3.3:69822780-69822799 | None:intergenic | 20.0% |
| !! | TATATATACCAATAGTTTAC+TGG | - | chr3.3:69822965-69822984 | None:intergenic | 20.0% |
| !!! | AATGATATTTTGACAACTTT+TGG | + | chr3.3:69822539-69822558 | MS.gene044369:intron | 20.0% |
| ! | AACAATAGATAGAACATAGA+AGG | - | chr3.3:69822748-69822767 | None:intergenic | 25.0% |
| ! | AACATCATCAGTTATGTAAA+TGG | - | chr3.3:69822877-69822896 | None:intergenic | 25.0% |
| ! | AATAAGAAGTGAGAAAAACA+AGG | + | chr3.3:69822636-69822655 | MS.gene044369:intron | 25.0% |
| ! | ACATCATCAGTTATGTAAAT+GGG | - | chr3.3:69822876-69822895 | None:intergenic | 25.0% |
| ! | CTTGAAAAATAAACGAAGAT+TGG | + | chr3.3:69822279-69822298 | MS.gene044369:intron | 25.0% |
| ! | TTCTTATTGGTCAAAAACAA+TGG | - | chr3.3:69822624-69822643 | None:intergenic | 25.0% |
| !!! | CAATCTTCGTTTATTTTTCA+AGG | - | chr3.3:69822281-69822300 | None:intergenic | 25.0% |
| !!! | TTGTTTTTCTCACTTCTTAT+TGG | - | chr3.3:69822637-69822656 | None:intergenic | 25.0% |
| !!! | TTTACTGATTCATAACCAAA+AGG | - | chr3.3:69822239-69822258 | None:intergenic | 25.0% |
| AAAACAATGGAGAGAGAAAA+AGG | - | chr3.3:69822611-69822630 | None:intergenic | 30.0% | |
| ATCAAAATCAGATTCGGATT+TGG | + | chr3.3:69822450-69822469 | MS.gene044369:intron | 30.0% | |
| GCTGAAATCAAAATCAGATT+CGG | + | chr3.3:69822444-69822463 | MS.gene044369:intron | 30.0% | |
| GTTCAAAACCAGTAAACTAT+TGG | + | chr3.3:69822954-69822973 | MS.gene044369:intron | 30.0% | |
| TAGCTTGAAAGGTAATGTAA+TGG | - | chr3.3:69823098-69823117 | None:intergenic | 30.0% | |
| TATCATTACTCCTGTGTATA+AGG | + | chr3.3:69822694-69822713 | MS.gene044369:intron | 30.0% | |
| TATGAATCAGTAAAGAGGAT+TGG | + | chr3.3:69822245-69822264 | MS.gene044369:CDS | 30.0% | |
| TCAACAAAGTTGTCATCAAA+TGG | + | chr3.3:69822662-69822681 | MS.gene044369:intron | 30.0% | |
| TTGGTTATGAATCAGTAAAG+AGG | + | chr3.3:69822240-69822259 | MS.gene044369:CDS | 30.0% | |
| AGAAGAAGAGGAAGAAAGAA+AGG | + | chr3.3:69822498-69822517 | MS.gene044369:intron | 35.0% | |
| CGGAATTCCATCAAAGATTT+CGG | - | chr3.3:69823166-69823185 | None:intergenic | 35.0% | |
| GAAGATTGGTGTATTCGTTT+AGG | + | chr3.3:69822293-69822312 | MS.gene044369:intron | 35.0% | |
| TAAAACCTTGAACGCATTGA+CGG | - | chr3.3:69823074-69823093 | None:intergenic | 35.0% | |
| ! | AAGTGAAACAAAGGGTACAA+AGG | - | chr3.3:69822771-69822790 | None:intergenic | 35.0% |
| !! | TGTAGCATCTTTAGCTTGAA+AGG | - | chr3.3:69823109-69823128 | None:intergenic | 35.0% |
| AGACAAGTACAATGCTGATC+AGG | + | chr3.3:69822932-69822951 | MS.gene044369:CDS | 40.0% | |
| AGAGGAAGAAAGAAAGGTGA+TGG | + | chr3.3:69822504-69822523 | MS.gene044369:intron | 40.0% | |
| ATAGAAGGAAACGTACAGTC+AGG | - | chr3.3:69822733-69822752 | None:intergenic | 40.0% | |
| CGACATTCTGATCTGTAACA+AGG | - | chr3.3:69822352-69822371 | None:intergenic | 40.0% | |
| GTACTTGTCTAACGCAATCT+TGG | - | chr3.3:69822922-69822941 | None:intergenic | 40.0% | |
| GTTACAGATCAGAATGTCGA+TGG | + | chr3.3:69822353-69822372 | MS.gene044369:intron | 40.0% | |
| TTTGATGGAATTCCGTCAGA+TGG | + | chr3.3:69823171-69823190 | MS.gene044369:CDS | 40.0% | |
| ! | AAAACCTTGAACGCATTGAC+GGG | - | chr3.3:69823073-69823092 | None:intergenic | 40.0% |
| ! | TTGTGTTTGATGACCTTGTC+AGG | + | chr3.3:69823043-69823062 | MS.gene044369:CDS | 40.0% |
| ATACCGCCACATAAACCTTG+TGG | - | chr3.3:69822849-69822868 | None:intergenic | 45.0% | |
| CAGTCCTGCTATAACTTTCC+AGG | + | chr3.3:69823140-69823159 | MS.gene044369:CDS | 45.0% | |
| CTGGGTGAAGAAAGAAGAAG+AGG | + | chr3.3:69822486-69822505 | MS.gene044369:intron | 45.0% | |
| GAAAGTTATAGCAGGACTGC+TGG | - | chr3.3:69823139-69823158 | None:intergenic | 45.0% | |
| GATCAGAATGTCGATGGAGT+CGG | + | chr3.3:69822359-69822378 | MS.gene044369:intron | 45.0% | |
| GTCTAACGCAATCTTGGAGA+AGG | - | chr3.3:69822916-69822935 | None:intergenic | 45.0% | |
| TGGAGAAGGTAATGAGACGA+GGG | - | chr3.3:69822902-69822921 | None:intergenic | 45.0% | |
| TTCCAGGCCGAAATCTTTGA+TGG | + | chr3.3:69823156-69823175 | MS.gene044369:CDS | 45.0% | |
| TTCCATCAAAGATTTCGGCC+TGG | - | chr3.3:69823161-69823180 | None:intergenic | 45.0% | |
| TTGGAGAAGGTAATGAGACG+AGG | - | chr3.3:69822903-69822922 | None:intergenic | 45.0% | |
| TTTCTTCACCCAGTCCAGTT+CGG | - | chr3.3:69822479-69822498 | None:intergenic | 45.0% | |
| ATGGCACACGAACTAACCAC+AGG | - | chr3.3:69823201-69823220 | None:intergenic | 50.0% | |
| CCTCCACAAGGTTTATGTGG+CGG | + | chr3.3:69822843-69822862 | MS.gene044369:CDS | 50.0% | |
| CGCCACATAAACCTTGTGGA+GGG | - | chr3.3:69822845-69822864 | None:intergenic | 50.0% | |
| CTCTTTCCTCACAGCTTTCC+CGG | - | chr3.3:69822417-69822436 | None:intergenic | 50.0% | |
| GACCCTCCACAAGGTTTATG+TGG | + | chr3.3:69822840-69822859 | MS.gene044369:CDS | 50.0% | |
| GTTATAGCAGGACTGCTGGA+AGG | - | chr3.3:69823135-69823154 | None:intergenic | 50.0% | |
| TCAACCCGTCAATGCGTTCA+AGG | + | chr3.3:69823066-69823085 | MS.gene044369:CDS | 50.0% | |
| TCGGCCTGGAAAGTTATAGC+AGG | - | chr3.3:69823147-69823166 | None:intergenic | 50.0% | |
| TCTTTCCTCACAGCTTTCCC+GGG | - | chr3.3:69822416-69822435 | None:intergenic | 50.0% | |
| TTCTTCACCCAGTCCAGTTC+GGG | - | chr3.3:69822478-69822497 | None:intergenic | 50.0% | |
| ! | ATTTGGAACCCGAACTGGAC+TGG | + | chr3.3:69822467-69822486 | MS.gene044369:intron | 50.0% |
| ! | TTCGGATTTGGAACCCGAAC+TGG | + | chr3.3:69822462-69822481 | MS.gene044369:intron | 50.0% |
| ! | TTTGGAACCCGAACTGGACT+GGG | + | chr3.3:69822468-69822487 | MS.gene044369:intron | 50.0% |
| !! | ATTGACGGGTTGACCTGACA+AGG | - | chr3.3:69823059-69823078 | None:intergenic | 50.0% |
| ACAGCTTTCCCGGGTAAGGT+TGG | - | chr3.3:69822407-69822426 | None:intergenic | 55.0% | |
| CAGCTTTCCCGGGTAAGGTT+GGG | - | chr3.3:69822406-69822425 | None:intergenic | 55.0% | |
| CCGCCACATAAACCTTGTGG+AGG | - | chr3.3:69822846-69822865 | None:intergenic | 55.0% | |
| GAGTCGGATACCTGCTTGCA+CGG | + | chr3.3:69822375-69822394 | MS.gene044369:CDS | 55.0% | |
| GCACACGAACTAACCACAGG+CGG | - | chr3.3:69823198-69823217 | None:intergenic | 55.0% | |
| !! | CGAGAGCGCTCCTTATACAC+AGG | - | chr3.3:69822707-69822726 | None:intergenic | 55.0% |
| ACCACAGGCGGTCCATCTGA+CGG | - | chr3.3:69823186-69823205 | None:intergenic | 60.0% | |
| CCTCACAGCTTTCCCGGGTA+AGG | - | chr3.3:69822411-69822430 | None:intergenic | 60.0% | |
| CCTTACCCGGGAAAGCTGTG+AGG | + | chr3.3:69822408-69822427 | MS.gene044369:intron | 60.0% | |
| CGGCATCACCCAACCTTACC+CGG | + | chr3.3:69822395-69822414 | MS.gene044369:CDS | 60.0% | |
| GACGCTATCGACCCTCCACA+AGG | + | chr3.3:69822831-69822850 | MS.gene044369:CDS | 60.0% | |
| GGCATCACCCAACCTTACCC+GGG | + | chr3.3:69822396-69822415 | MS.gene044369:CDS | 60.0% | |
| TTGGGTGATGCCGTGCAAGC+AGG | - | chr3.3:69822388-69822407 | None:intergenic | 60.0% | |
| TCCGTCAGATGGACCGCCTG+TGG | + | chr3.3:69823182-69823201 | MS.gene044369:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69822227 | 69823233 | 69822227 | ID=MS.gene044369 |
| chr3.3 | mRNA | 69822227 | 69823233 | 69822227 | ID=MS.gene044369.t1;Parent=MS.gene044369 |
| chr3.3 | exon | 69822227 | 69822266 | 69822227 | ID=MS.gene044369.t1.exon1;Parent=MS.gene044369.t1 |
| chr3.3 | CDS | 69822227 | 69822266 | 69822227 | ID=cds.MS.gene044369.t1;Parent=MS.gene044369.t1 |
| chr3.3 | exon | 69822360 | 69822424 | 69822360 | ID=MS.gene044369.t1.exon2;Parent=MS.gene044369.t1 |
| chr3.3 | CDS | 69822360 | 69822424 | 69822360 | ID=cds.MS.gene044369.t1;Parent=MS.gene044369.t1 |
| chr3.3 | exon | 69822825 | 69822953 | 69822825 | ID=MS.gene044369.t1.exon3;Parent=MS.gene044369.t1 |
| chr3.3 | CDS | 69822825 | 69822953 | 69822825 | ID=cds.MS.gene044369.t1;Parent=MS.gene044369.t1 |
| chr3.3 | exon | 69823033 | 69823233 | 69823033 | ID=MS.gene044369.t1.exon4;Parent=MS.gene044369.t1 |
| chr3.3 | CDS | 69823033 | 69823233 | 69823033 | ID=cds.MS.gene044369.t1;Parent=MS.gene044369.t1 |
| Gene Sequence |
| Protein sequence |
