Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044375.t1 | AES71816.1 | 67.8 | 202 | 23 | 4 | 1 | 169 | 1 | 193 | 5.20E-61 | 244.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044375.t1 | G7J9H7 | 67.8 | 202 | 23 | 4 | 1 | 169 | 1 | 193 | 3.7e-61 | 244.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044375 | MS.gene049263 | PPI |
| MS.gene044376 | MS.gene044375 | PPI |
| MS.gene044363 | MS.gene044375 | PPI |
| MS.gene70430 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene77468 | PPI |
| MS.gene044375 | MS.gene71531 | PPI |
| MS.gene70418 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene044323 | PPI |
| MS.gene044375 | MS.gene71282 | PPI |
| MS.gene70443 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene71284 | PPI |
| MS.gene044375 | MS.gene045214 | PPI |
| MS.gene77472 | MS.gene044375 | PPI |
| MS.gene77466 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene71524 | PPI |
| MS.gene044375 | MS.gene71528 | PPI |
| MS.gene044375 | MS.gene70424 | PPI |
| MS.gene25704 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene77464 | PPI |
| MS.gene044361 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene044326 | PPI |
| MS.gene79115 | MS.gene044375 | PPI |
| MS.gene044370 | MS.gene044375 | PPI |
| MS.gene044375 | MS.gene70427 | PPI |
| MS.gene044375 | MS.gene70476 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044375.t1 | MTR_3g083080 | 67.822 | 202 | 23 | 4 | 1 | 169 | 1 | 193 | 1.82e-84 | 249 |
| MS.gene044375.t1 | MTR_3g083030 | 44.954 | 218 | 57 | 5 | 1 | 161 | 1 | 212 | 2.54e-47 | 155 |
| MS.gene044375.t1 | MTR_3g083050 | 47.692 | 195 | 62 | 4 | 1 | 161 | 1 | 189 | 3.43e-46 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGATTAGTGACTTCTTTA+TGG | 0.084650 | 3.3:+69907132 | MS.gene044375:CDS |
| TTCGGTTGCAATCCCGTTAC+AGG | 0.190009 | 3.3:-69907064 | None:intergenic |
| CCGGAAAACTGTGATGAAAA+AGG | 0.252165 | 3.3:+69907034 | MS.gene044375:CDS |
| CGCCCACACATAAACATAAA+AGG | 0.266595 | 3.3:-69907172 | None:intergenic |
| TCATATTTCTTTCTCCGTTT+CGG | 0.317369 | 3.3:-69907082 | None:intergenic |
| GCGATTTACGTATAAGAATA+AGG | 0.390721 | 3.3:+69907192 | MS.gene044375:CDS |
| TCTTGTTTCATACTTGTGCT+TGG | 0.413432 | 3.3:-69907528 | None:intergenic |
| ATAAATGCTAACAGGTCATT+AGG | 0.415829 | 3.3:+69907674 | MS.gene044375:intron |
| TTAGGCCTGTGCAAAATAAC+CGG | 0.445160 | 3.3:+69907692 | MS.gene044375:CDS |
| GAAAATTATAGCAGAGATGT+TGG | 0.447031 | 3.3:-69907506 | None:intergenic |
| CGGCGTCACCCAACCTTCTC+CGG | 0.453638 | 3.3:+69907015 | MS.gene044375:CDS |
| AACGGAGAAAGAAATATGAA+TGG | 0.461826 | 3.3:+69907086 | MS.gene044375:CDS |
| AAAGGGAATCAACCTGTAAC+GGG | 0.471848 | 3.3:+69907052 | MS.gene044375:CDS |
| TGCAGAGGGGAGTGAGTTCA+AGG | 0.477142 | 3.3:-69907267 | None:intergenic |
| CACGACGGCGGCAAAATATG+TGG | 0.492216 | 3.3:+69908183 | MS.gene044375:CDS |
| CGGAGAAGGTTGGGTGACGC+CGG | 0.498648 | 3.3:-69907014 | None:intergenic |
| TTCTTGTTCGTGTGCTTGAA+AGG | 0.503496 | 3.3:-69907464 | None:intergenic |
| AATGTCGATGGAATTCAATA+CGG | 0.514369 | 3.3:+69906985 | None:intergenic |
| GGAGAAGGTTGGGTGACGCC+GGG | 0.516300 | 3.3:-69907013 | None:intergenic |
| TAACGGGATTGCAACCGAAA+CGG | 0.516954 | 3.3:+69907068 | MS.gene044375:CDS |
| ACGGAGAAAGAAATATGAAT+GGG | 0.520163 | 3.3:+69907087 | MS.gene044375:CDS |
| CAAGTACAATGCTGAGAACC+AGG | 0.527031 | 3.3:+69907303 | MS.gene044375:CDS |
| ATACATACAACCAGGTCTCC+AGG | 0.532645 | 3.3:-69907434 | None:intergenic |
| AAGATCATCGGTTAAGTCGA+TGG | 0.533943 | 3.3:-69907242 | None:intergenic |
| GAATTCAATACGGTTTCGCC+CGG | 0.538537 | 3.3:+69906995 | MS.gene044375:CDS |
| TTGACATTGTGAAGACAACC+TGG | 0.541774 | 3.3:+69907416 | MS.gene044375:CDS |
| TTTGCCGCCGTCGTGCAAAG+AGG | 0.560812 | 3.3:-69908175 | None:intergenic |
| AAAAGGGAATCAACCTGTAA+CGG | 0.561384 | 3.3:+69907051 | MS.gene044375:CDS |
| GTCTAACACAAACTTGCAGA+GGG | 0.571934 | 3.3:-69907281 | None:intergenic |
| ATTATAGCAGAGATGTTGGA+AGG | 0.576865 | 3.3:-69907502 | None:intergenic |
| AGATCATCGGTTAAGTCGAT+GGG | 0.584455 | 3.3:-69907241 | None:intergenic |
| GAGTTCAAGGCAAAGATCAT+CGG | 0.594092 | 3.3:-69907254 | None:intergenic |
| GTGAAGACAACCTGGAGACC+TGG | 0.604519 | 3.3:+69907424 | MS.gene044375:CDS |
| TCCGTGTTACAGAATGTCGA+TGG | 0.606334 | 3.3:+69906973 | None:intergenic |
| GACGACAGAAGCTTTGCGCA+CGG | 0.608634 | 3.3:+69908141 | MS.gene044375:CDS |
| ATAACCTCTTTGCACGACGG+CGG | 0.614814 | 3.3:+69908171 | MS.gene044375:CDS |
| GTTGACAGAAACTTTGCTCA+CGG | 0.614889 | 3.3:+69908117 | MS.gene044375:CDS |
| ACGGCGGCAAAATATGTGGA+TGG | 0.637748 | 3.3:+69908187 | MS.gene044375:CDS |
| TGTCTAACACAAACTTGCAG+AGG | 0.637970 | 3.3:-69907282 | None:intergenic |
| GATATAACCTCTTTGCACGA+CGG | 0.649315 | 3.3:+69908168 | MS.gene044375:CDS |
| GTAATGTAATACATACAACC+AGG | 0.734534 | 3.3:-69907442 | None:intergenic |
| GACAGAAGCTTTGCGCACGG+CGG | 0.747493 | 3.3:+69908144 | MS.gene044375:CDS |
| TCTAACACAAACTTGCAGAG+GGG | 0.776478 | 3.3:-69907280 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATTTGGTTTTAAAATTTA+TGG | - | chr3.3:69907764-69907783 | None:intergenic | 10.0% |
| !!! | AAAAATTGGTAATAGTTTTT+TGG | - | chr3.3:69907855-69907874 | None:intergenic | 15.0% |
| !!! | AAAATTGGTAATAGTTTTTT+GGG | - | chr3.3:69907854-69907873 | None:intergenic | 15.0% |
| !!! | AATTTATGGTTATTTTAGAA+TGG | - | chr3.3:69907750-69907769 | None:intergenic | 15.0% |
| ! | ATCAACCAAACCATTTAAAA+TGG | + | chr3.3:69907782-69907801 | MS.gene044375:intron | 25.0% |
| !! | ACTAATTTTTCTCCTGATTT+AGG | + | chr3.3:69907375-69907394 | MS.gene044375:intron | 25.0% |
| !! | CATCATGTTTAATCTTTTCT+TGG | + | chr3.3:69907645-69907664 | MS.gene044375:intron | 25.0% |
| !! | TATAACCGATCCATTTTAAA+TGG | - | chr3.3:69907795-69907814 | None:intergenic | 25.0% |
| !!! | TAAATGGTTTGGTTGATATT+TGG | - | chr3.3:69907779-69907798 | None:intergenic | 25.0% |
| !!! | TGGTTATTTTAGAATGGTTA+CGG | - | chr3.3:69907744-69907763 | None:intergenic | 25.0% |
| !!! | TTTTGGGCTTTTTCTTTTTA+GGG | - | chr3.3:69907838-69907857 | None:intergenic | 25.0% |
| !!! | TTTTTCTTTTTAGGGATAAC+CGG | - | chr3.3:69907830-69907849 | None:intergenic | 25.0% |
| !!! | TTTTTGGGCTTTTTCTTTTT+AGG | - | chr3.3:69907839-69907858 | None:intergenic | 25.0% |
| AACGGAGAAAGAAATATGAA+TGG | + | chr3.3:69907086-69907105 | MS.gene044375:CDS | 30.0% | |
| ACGGAGAAAGAAATATGAAT+GGG | + | chr3.3:69907087-69907106 | MS.gene044375:CDS | 30.0% | |
| ATAAATGCTAACAGGTCATT+AGG | + | chr3.3:69907674-69907693 | MS.gene044375:intron | 30.0% | |
| CAAAATTTGCAACCTAAATC+AGG | - | chr3.3:69907390-69907409 | None:intergenic | 30.0% | |
| GAAAATTATAGCAGAGATGT+TGG | - | chr3.3:69907509-69907528 | None:intergenic | 30.0% | |
| GCGATTTACGTATAAGAATA+AGG | + | chr3.3:69907192-69907211 | MS.gene044375:CDS | 30.0% | |
| GGAGATTTATAAATGCTAAC+AGG | + | chr3.3:69907666-69907685 | MS.gene044375:intron | 30.0% | |
| GGTTATAAACCATTGTTAAC+CGG | + | chr3.3:69907808-69907827 | MS.gene044375:intron | 30.0% | |
| GTAATGTAATACATACAACC+AGG | - | chr3.3:69907445-69907464 | None:intergenic | 30.0% | |
| TATGGATTTACTACTCAAAC+CGG | - | chr3.3:69907714-69907733 | None:intergenic | 30.0% | |
| TCATATTTCTTTCTCCGTTT+CGG | - | chr3.3:69907085-69907104 | None:intergenic | 30.0% | |
| TGAGATTAGTGACTTCTTTA+TGG | + | chr3.3:69907132-69907151 | MS.gene044375:CDS | 30.0% | |
| ! | AAGCCTTTTATGTTTATGTG+TGG | + | chr3.3:69907169-69907188 | MS.gene044375:CDS | 30.0% |
| ! | AGCCTTTTATGTTTATGTGT+GGG | + | chr3.3:69907170-69907189 | MS.gene044375:CDS | 30.0% |
| ! | CCAAACCATTTAAAATGGAT+CGG | + | chr3.3:69907787-69907806 | MS.gene044375:intron | 30.0% |
| ! | TGAGTTGAAAGCAGAAAAAT+TGG | - | chr3.3:69907869-69907888 | None:intergenic | 30.0% |
| !! | TTCTTGTAATTTGTTGAACC+TGG | - | chr3.3:69907324-69907343 | None:intergenic | 30.0% |
| !!! | GGTTATTTTAGAATGGTTAC+GGG | - | chr3.3:69907743-69907762 | None:intergenic | 30.0% |
| AAAAGGGAATCAACCTGTAA+CGG | + | chr3.3:69907051-69907070 | MS.gene044375:CDS | 35.0% | |
| AAGCACAAGTATGAAACAAG+AGG | + | chr3.3:69907530-69907549 | MS.gene044375:intron | 35.0% | |
| ATTATAGCAGAGATGTTGGA+AGG | - | chr3.3:69907505-69907524 | None:intergenic | 35.0% | |
| CGGAAAACTGTGATGAAAAA+GGG | + | chr3.3:69907035-69907054 | MS.gene044375:CDS | 35.0% | |
| ! | CCGATCCATTTTAAATGGTT+TGG | - | chr3.3:69907790-69907809 | None:intergenic | 35.0% |
| ! | TCTTGTTTCATACTTGTGCT+TGG | - | chr3.3:69907531-69907550 | None:intergenic | 35.0% |
| !! | CCTTTTTCATCACAGTTTTC+CGG | - | chr3.3:69907037-69907056 | None:intergenic | 35.0% |
| !!! | TTTTAGAATGGTTACGGGTA+TGG | - | chr3.3:69907738-69907757 | None:intergenic | 35.0% |
| AAAGGGAATCAACCTGTAAC+GGG | + | chr3.3:69907052-69907071 | MS.gene044375:CDS | 40.0% | |
| AAGTATGAAACAAGAGGCCT+GGG | + | chr3.3:69907536-69907555 | MS.gene044375:intron | 40.0% | |
| AATGGTTACGGGTATGGTTA+TGG | - | chr3.3:69907732-69907751 | None:intergenic | 40.0% | |
| ACCTAACACGTACATCATCA+TGG | + | chr3.3:69907592-69907611 | MS.gene044375:intron | 40.0% | |
| CCGGAAAACTGTGATGAAAA+AGG | + | chr3.3:69907034-69907053 | MS.gene044375:CDS | 40.0% | |
| CGCCCACACATAAACATAAA+AGG | - | chr3.3:69907175-69907194 | None:intergenic | 40.0% | |
| GAGTTCAAGGCAAAGATCAT+CGG | - | chr3.3:69907257-69907276 | None:intergenic | 40.0% | |
| GATATAACCTCTTTGCACGA+CGG | + | chr3.3:69908168-69908187 | MS.gene044375:CDS | 40.0% | |
| GTCTAACACAAACTTGCAGA+GGG | - | chr3.3:69907284-69907303 | None:intergenic | 40.0% | |
| GTTGACAGAAACTTTGCTCA+CGG | + | chr3.3:69908117-69908136 | MS.gene044375:CDS | 40.0% | |
| TAGGGATAACCGGTTAACAA+TGG | - | chr3.3:69907820-69907839 | None:intergenic | 40.0% | |
| TCTAACACAAACTTGCAGAG+GGG | - | chr3.3:69907283-69907302 | None:intergenic | 40.0% | |
| TGTCTAACACAAACTTGCAG+AGG | - | chr3.3:69907285-69907304 | None:intergenic | 40.0% | |
| TTAGGCCTGTGCAAAATAAC+CGG | + | chr3.3:69907692-69907711 | MS.gene044375:CDS | 40.0% | |
| TTCTTGTTCGTGTGCTTGAA+AGG | - | chr3.3:69907467-69907486 | None:intergenic | 40.0% | |
| TTGACATTGTGAAGACAACC+TGG | + | chr3.3:69907416-69907435 | MS.gene044375:CDS | 40.0% | |
| ! | AAGATCATCGGTTAAGTCGA+TGG | - | chr3.3:69907245-69907264 | None:intergenic | 40.0% |
| ! | ACCATGATGATGTACGTGTT+AGG | - | chr3.3:69907596-69907615 | None:intergenic | 40.0% |
| ! | AGATCATCGGTTAAGTCGAT+GGG | - | chr3.3:69907244-69907263 | None:intergenic | 40.0% |
| ! | TGATGATGTACGTGTTAGGT+AGG | - | chr3.3:69907592-69907611 | None:intergenic | 40.0% |
| !! | TCAAACCGGTTATTTTGCAC+AGG | - | chr3.3:69907700-69907719 | None:intergenic | 40.0% |
| ATACATACAACCAGGTCTCC+AGG | - | chr3.3:69907437-69907456 | None:intergenic | 45.0% | |
| CAAGTACAATGCTGAGAACC+AGG | + | chr3.3:69907303-69907322 | MS.gene044375:CDS | 45.0% | |
| CAAGTATGAAACAAGAGGCC+TGG | + | chr3.3:69907535-69907554 | MS.gene044375:intron | 45.0% | |
| GAATTCAATACGGTTTCGCC+CGG | + | chr3.3:69906995-69907014 | MS.gene044375:CDS | 45.0% | |
| TAACGGGATTGCAACCGAAA+CGG | + | chr3.3:69907068-69907087 | MS.gene044375:CDS | 45.0% | |
| TACGTGTTAGGTAGGCTTCA+TGG | - | chr3.3:69907584-69907603 | None:intergenic | 45.0% | |
| ! | ACAGTTTTCCGGAGAAGGTT+GGG | - | chr3.3:69907026-69907045 | None:intergenic | 45.0% |
| ! | TCATCACAGTTTTCCGGAGA+AGG | - | chr3.3:69907031-69907050 | None:intergenic | 45.0% |
| ACGGCGGCAAAATATGTGGA+TGG | + | chr3.3:69908187-69908206 | MS.gene044375:CDS | 50.0% | |
| ATAACCTCTTTGCACGACGG+CGG | + | chr3.3:69908171-69908190 | MS.gene044375:CDS | 50.0% | |
| ATGGCACATGACTTGACCTC+AGG | - | chr3.3:69907565-69907584 | None:intergenic | 50.0% | |
| GACGGAGATGAAAACGCAAG+AGG | + | chr3.3:69907918-69907937 | MS.gene044375:intron | 50.0% | |
| GGAGATGAAAACGCAAGAGG+AGG | + | chr3.3:69907921-69907940 | MS.gene044375:intron | 50.0% | |
| TTCAACTCACTCCGATGACG+TGG | + | chr3.3:69907880-69907899 | MS.gene044375:intron | 50.0% | |
| TTCGGTTGCAATCCCGTTAC+AGG | - | chr3.3:69907067-69907086 | None:intergenic | 50.0% | |
| ! | CACAGTTTTCCGGAGAAGGT+TGG | - | chr3.3:69907027-69907046 | None:intergenic | 50.0% |
| CACGACGGCGGCAAAATATG+TGG | + | chr3.3:69908183-69908202 | MS.gene044375:CDS | 55.0% | |
| GACGACAGAAGCTTTGCGCA+CGG | + | chr3.3:69908141-69908160 | MS.gene044375:CDS | 55.0% | |
| GCACATGACTTGACCTCAGG+TGG | - | chr3.3:69907562-69907581 | None:intergenic | 55.0% | |
| GTGAAGACAACCTGGAGACC+TGG | + | chr3.3:69907424-69907443 | MS.gene044375:CDS | 55.0% | |
| TCACAGAGATGACTCCGACG+AGG | + | chr3.3:69908025-69908044 | MS.gene044375:intron | 55.0% | |
| TGCAGAGGGGAGTGAGTTCA+AGG | - | chr3.3:69907270-69907289 | None:intergenic | 55.0% | |
| CTCACTCCGATGACGTGGCA+CGG | + | chr3.3:69907885-69907904 | MS.gene044375:intron | 60.0% | |
| GACAGAAGCTTTGCGCACGG+CGG | + | chr3.3:69908144-69908163 | MS.gene044375:CDS | 60.0% | |
| TAACCGCCGTGCCACGTCAT+CGG | - | chr3.3:69907894-69907913 | None:intergenic | 60.0% | |
| TGGCACGGCGGTTACATGGA+CGG | + | chr3.3:69907900-69907919 | MS.gene044375:intron | 60.0% | |
| TTTGCCGCCGTCGTGCAAAG+AGG | - | chr3.3:69908178-69908197 | None:intergenic | 60.0% | |
| ACTCCGATGACGTGGCACGG+CGG | + | chr3.3:69907888-69907907 | MS.gene044375:intron | 65.0% | |
| CGGAGAAGGTTGGGTGACGC+CGG | - | chr3.3:69907017-69907036 | None:intergenic | 65.0% | |
| CGGCGTCACCCAACCTTCTC+CGG | + | chr3.3:69907015-69907034 | MS.gene044375:CDS | 65.0% | |
| CTCCGACGAGGTGTGCACGA+CGG | + | chr3.3:69908037-69908056 | MS.gene044375:intron | 65.0% | |
| GACGTGGCACGGCGGTTACA+TGG | + | chr3.3:69907896-69907915 | MS.gene044375:intron | 65.0% | |
| GACTTGACCTCAGGTGGCCC+AGG | - | chr3.3:69907556-69907575 | None:intergenic | 65.0% | |
| GGAGAAGGTTGGGTGACGCC+GGG | - | chr3.3:69907016-69907035 | None:intergenic | 65.0% | |
| CAAGAGGCCTGGGCCACCTG+AGG | + | chr3.3:69907546-69907565 | MS.gene044375:intron | 70.0% | |
| CGCCGTCGTGCACACCTCGT+CGG | - | chr3.3:69908042-69908061 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69906986 | 69908215 | 69906986 | ID=MS.gene044375 |
| chr3.3 | mRNA | 69906986 | 69908215 | 69906986 | ID=MS.gene044375.t1;Parent=MS.gene044375 |
| chr3.3 | exon | 69906986 | 69907324 | 69906986 | ID=MS.gene044375.t1.exon1;Parent=MS.gene044375.t1 |
| chr3.3 | CDS | 69906986 | 69907324 | 69906986 | ID=cds.MS.gene044375.t1;Parent=MS.gene044375.t1 |
| chr3.3 | exon | 69907397 | 69907537 | 69907397 | ID=MS.gene044375.t1.exon2;Parent=MS.gene044375.t1 |
| chr3.3 | CDS | 69907397 | 69907537 | 69907397 | ID=cds.MS.gene044375.t1;Parent=MS.gene044375.t1 |
| chr3.3 | exon | 69907688 | 69907713 | 69907688 | ID=MS.gene044375.t1.exon3;Parent=MS.gene044375.t1 |
| chr3.3 | CDS | 69907688 | 69907713 | 69907688 | ID=cds.MS.gene044375.t1;Parent=MS.gene044375.t1 |
| chr3.3 | exon | 69908113 | 69908215 | 69908113 | ID=MS.gene044375.t1.exon4;Parent=MS.gene044375.t1 |
| chr3.3 | CDS | 69908113 | 69908215 | 69908113 | ID=cds.MS.gene044375.t1;Parent=MS.gene044375.t1 |
| Gene Sequence |
| Protein sequence |
