Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044377.t1 | RHN69093.1 | 74.7 | 83 | 18 | 1 | 8 | 90 | 154 | 233 | 3.50E-25 | 124 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044377.t1 | G7J9I4 | 74.7 | 83 | 18 | 1 | 8 | 90 | 133 | 212 | 2.5e-25 | 124.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044377 | MS.gene71282 | PPI |
| MS.gene044377 | MS.gene71528 | PPI |
| MS.gene044377 | MS.gene71284 | PPI |
| MS.gene044377 | MS.gene045214 | PPI |
| MS.gene044377 | MS.gene77466 | PPI |
| MS.gene044377 | MS.gene044361 | PPI |
| MS.gene77472 | MS.gene044377 | PPI |
| MS.gene70418 | MS.gene044377 | PPI |
| MS.gene044377 | MS.gene71524 | PPI |
| MS.gene044377 | MS.gene70424 | PPI |
| MS.gene044377 | MS.gene77464 | PPI |
| MS.gene044377 | MS.gene049263 | PPI |
| MS.gene044377 | MS.gene044370 | PPI |
| MS.gene044377 | MS.gene044326 | PPI |
| MS.gene044377 | MS.gene79115 | PPI |
| MS.gene044377 | MS.gene70427 | PPI |
| MS.gene044377 | MS.gene70476 | PPI |
| MS.gene044377 | MS.gene044323 | PPI |
| MS.gene70443 | MS.gene044377 | PPI |
| MS.gene044377 | MS.gene044376 | PPI |
| MS.gene044363 | MS.gene044377 | PPI |
| MS.gene044377 | MS.gene70430 | PPI |
| MS.gene044377 | MS.gene25704 | PPI |
| MS.gene044377 | MS.gene77468 | PPI |
| MS.gene044377 | MS.gene71531 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044377.t1 | MTR_3g083150 | 74.699 | 83 | 18 | 1 | 8 | 90 | 133 | 212 | 3.62e-36 | 121 |
| MS.gene044377.t1 | MTR_3g083100 | 52.222 | 90 | 31 | 2 | 9 | 90 | 89 | 174 | 8.92e-22 | 84.0 |
| MS.gene044377.t1 | MTR_3g083080 | 46.316 | 95 | 37 | 5 | 1 | 89 | 119 | 205 | 7.77e-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGGTAGCATCTTCTGGTTT+TGG | 0.308463 | 3.3:-69923445 | None:intergenic |
| TGAACATGGGTAGCATCTTC+TGG | 0.357963 | 3.3:-69923451 | None:intergenic |
| TCGCCTCTCCAAGCTTGCTT+TGG | 0.392937 | 3.3:+69923214 | MS.gene044377:CDS |
| CACGACTTGATCACAGGTTC+AGG | 0.456720 | 3.3:-69923526 | None:intergenic |
| GTACTTCTCCAAAGCAAGCT+TGG | 0.468678 | 3.3:-69923222 | None:intergenic |
| ATCATCATCGGTAATGTCCA+TGG | 0.485528 | 3.3:-69923192 | None:intergenic |
| CGGGCCTGAAAAGTTATAGC+AGG | 0.514776 | 3.3:-69923487 | None:intergenic |
| ACCCGTGTGCATATTCCTCT+TGG | 0.518195 | 3.3:+69923394 | MS.gene044377:CDS |
| AAAAGTTATAGCAGGGCAGT+TGG | 0.545343 | 3.3:-69923479 | None:intergenic |
| TGAATTTGATGTGCTCATCA+AGG | 0.546727 | 3.3:+69923369 | MS.gene044377:CDS |
| CTTGGAGAGGCGATCATCAT+CGG | 0.553632 | 3.3:-69923204 | None:intergenic |
| ATGGAACACGACTTGATCAC+AGG | 0.555050 | 3.3:-69923532 | None:intergenic |
| GTTATAGCAGGGCAGTTGGA+AGG | 0.582712 | 3.3:-69923475 | None:intergenic |
| CAGGCATACTGTCACAGACA+CGG | 0.585144 | 3.3:-69923507 | None:intergenic |
| GGCAGTTGGAAGGATGAACA+TGG | 0.590763 | 3.3:-69923465 | None:intergenic |
| TCATCATCGGTAATGTCCAT+GGG | 0.608264 | 3.3:-69923191 | None:intergenic |
| AGGCATACTGTCACAGACAC+GGG | 0.620581 | 3.3:-69923506 | None:intergenic |
| TCTCCAAAGCAAGCTTGGAG+AGG | 0.642137 | 3.3:-69923217 | None:intergenic |
| GGGCCTGAAAAGTTATAGCA+GGG | 0.658880 | 3.3:-69923486 | None:intergenic |
| TACCAAGAGGAATATGCACA+CGG | 0.664367 | 3.3:-69923396 | None:intergenic |
| GTAATATAATAAGTACCAAG+AGG | 0.676156 | 3.3:-69923409 | None:intergenic |
| GCAGTTGGAAGGATGAACAT+GGG | 0.704484 | 3.3:-69923464 | None:intergenic |
| ACCAAGAGGAATATGCACAC+GGG | 0.718548 | 3.3:-69923395 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGTTTCTCTTATTT+AGG | + | chr3.3:69923336-69923355 | MS.gene044377:intron | 15.0% |
| ! | ACATGGATATAATATAACAG+TGG | - | chr3.3:69923282-69923301 | None:intergenic | 25.0% |
| ! | GTAATATAATAAGTACCAAG+AGG | - | chr3.3:69923412-69923431 | None:intergenic | 25.0% |
| GATGAGTGAAAGAATAAACA+TGG | - | chr3.3:69923299-69923318 | None:intergenic | 30.0% | |
| TGGATATAATATAACAGTGG+TGG | - | chr3.3:69923279-69923298 | None:intergenic | 30.0% | |
| !!! | TGGTTTTGGTTTTGCTTTAA+AGG | - | chr3.3:69923434-69923453 | None:intergenic | 30.0% |
| TGAATTTGATGTGCTCATCA+AGG | + | chr3.3:69923369-69923388 | MS.gene044377:CDS | 35.0% | |
| AAAAGTTATAGCAGGGCAGT+TGG | - | chr3.3:69923482-69923501 | None:intergenic | 40.0% | |
| TACCAAGAGGAATATGCACA+CGG | - | chr3.3:69923399-69923418 | None:intergenic | 40.0% | |
| ACCAAGAGGAATATGCACAC+GGG | - | chr3.3:69923398-69923417 | None:intergenic | 45.0% | |
| ATGGAACACGACTTGATCAC+AGG | - | chr3.3:69923535-69923554 | None:intergenic | 45.0% | |
| GCAGTTGGAAGGATGAACAT+GGG | - | chr3.3:69923467-69923486 | None:intergenic | 45.0% | |
| GGGCCTGAAAAGTTATAGCA+GGG | - | chr3.3:69923489-69923508 | None:intergenic | 45.0% | |
| GTACTTCTCCAAAGCAAGCT+TGG | - | chr3.3:69923225-69923244 | None:intergenic | 45.0% | |
| TGAACATGGGTAGCATCTTC+TGG | - | chr3.3:69923454-69923473 | None:intergenic | 45.0% | |
| ! | ACAGTGGTGGTGAACTAGTT+TGG | - | chr3.3:69923266-69923285 | None:intergenic | 45.0% |
| ! | CTGCCCTGCTATAACTTTTC+AGG | + | chr3.3:69923483-69923502 | MS.gene044377:CDS | 45.0% |
| !!! | TGGGTAGCATCTTCTGGTTT+TGG | - | chr3.3:69923448-69923467 | None:intergenic | 45.0% |
| ACCCGTGTGCATATTCCTCT+TGG | + | chr3.3:69923394-69923413 | MS.gene044377:CDS | 50.0% | |
| AGGCATACTGTCACAGACAC+GGG | - | chr3.3:69923509-69923528 | None:intergenic | 50.0% | |
| CACGACTTGATCACAGGTTC+AGG | - | chr3.3:69923529-69923548 | None:intergenic | 50.0% | |
| CAGGCATACTGTCACAGACA+CGG | - | chr3.3:69923510-69923529 | None:intergenic | 50.0% | |
| CGGGCCTGAAAAGTTATAGC+AGG | - | chr3.3:69923490-69923509 | None:intergenic | 50.0% | |
| CTTGGAGAGGCGATCATCAT+CGG | - | chr3.3:69923207-69923226 | None:intergenic | 50.0% | |
| GGCAGTTGGAAGGATGAACA+TGG | - | chr3.3:69923468-69923487 | None:intergenic | 50.0% | |
| GTTATAGCAGGGCAGTTGGA+AGG | - | chr3.3:69923478-69923497 | None:intergenic | 50.0% | |
| TCTCCAAAGCAAGCTTGGAG+AGG | - | chr3.3:69923220-69923239 | None:intergenic | 50.0% | |
| ! | TCGCCTCTCCAAGCTTGCTT+TGG | + | chr3.3:69923214-69923233 | MS.gene044377:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69923194 | 69923564 | 69923194 | ID=MS.gene044377 |
| chr3.3 | mRNA | 69923194 | 69923564 | 69923194 | ID=MS.gene044377.t1;Parent=MS.gene044377 |
| chr3.3 | exon | 69923194 | 69923259 | 69923194 | ID=MS.gene044377.t1.exon1;Parent=MS.gene044377.t1 |
| chr3.3 | CDS | 69923194 | 69923259 | 69923194 | ID=cds.MS.gene044377.t1;Parent=MS.gene044377.t1 |
| chr3.3 | exon | 69923358 | 69923564 | 69923358 | ID=MS.gene044377.t1.exon2;Parent=MS.gene044377.t1 |
| chr3.3 | CDS | 69923358 | 69923564 | 69923358 | ID=cds.MS.gene044377.t1;Parent=MS.gene044377.t1 |
| Gene Sequence |
| Protein sequence |
