Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048707.t1 | XP_003600461.1 | 93.9 | 313 | 5 | 3 | 1 | 299 | 1 | 313 | 2.00E-170 | 608.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048707.t1 | Q9FKT5 | 80.5 | 313 | 46 | 4 | 2 | 299 | 3 | 315 | 7.1e-148 | 524.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048707.t1 | G7IY98 | 93.9 | 313 | 5 | 3 | 1 | 299 | 1 | 313 | 1.5e-170 | 608.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene048707 | MS.gene97980 | PPI |
| MS.gene008793 | MS.gene048707 | PPI |
| MS.gene52980 | MS.gene048707 | PPI |
| MS.gene042253 | MS.gene048707 | PPI |
| MS.gene048707 | MS.gene063165 | PPI |
| MS.gene004825 | MS.gene048707 | PPI |
| MS.gene046235 | MS.gene048707 | PPI |
| MS.gene056488 | MS.gene048707 | PPI |
| MS.gene048707 | MS.gene93058 | PPI |
| MS.gene93059 | MS.gene048707 | PPI |
| MS.gene048707 | MS.gene008794 | PPI |
| MS.gene95244 | MS.gene048707 | PPI |
| MS.gene048707 | MS.gene014281 | PPI |
| MS.gene97981 | MS.gene048707 | PPI |
| MS.gene68222 | MS.gene048707 | PPI |
| MS.gene064894 | MS.gene048707 | PPI |
| MS.gene048707 | MS.gene012608 | PPI |
| MS.gene33535 | MS.gene048707 | PPI |
| MS.gene048707 | MS.gene71431 | PPI |
| MS.gene066026 | MS.gene048707 | PPI |
| MS.gene056487 | MS.gene048707 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048707.t1 | MTR_3g061450 | 93.930 | 313 | 5 | 3 | 1 | 299 | 1 | 313 | 0.0 | 605 |
| MS.gene048707.t1 | MTR_4g095058 | 27.660 | 282 | 170 | 10 | 20 | 275 | 290 | 563 | 4.97e-16 | 78.6 |
| MS.gene048707.t1 | MTR_4g095058 | 30.178 | 169 | 90 | 7 | 8 | 158 | 402 | 560 | 7.41e-13 | 68.9 |
| MS.gene048707.t1 | MTR_4g095058 | 27.660 | 282 | 170 | 10 | 20 | 275 | 288 | 561 | 5.24e-16 | 78.6 |
| MS.gene048707.t1 | MTR_4g095058 | 30.178 | 169 | 90 | 7 | 8 | 158 | 400 | 558 | 7.00e-13 | 69.3 |
| MS.gene048707.t1 | MTR_3g106080 | 26.613 | 248 | 124 | 7 | 15 | 244 | 457 | 664 | 6.55e-16 | 78.2 |
| MS.gene048707.t1 | MTR_4g125710 | 24.627 | 268 | 169 | 7 | 13 | 251 | 54 | 317 | 1.23e-13 | 70.5 |
| MS.gene048707.t1 | MTR_5g016370 | 28.750 | 160 | 94 | 5 | 13 | 158 | 424 | 577 | 3.32e-13 | 70.1 |
| MS.gene048707.t1 | MTR_5g016370 | 23.867 | 331 | 193 | 11 | 17 | 293 | 245 | 570 | 1.04e-11 | 65.5 |
| MS.gene048707.t1 | MTR_5g016350 | 23.795 | 332 | 194 | 10 | 17 | 293 | 231 | 558 | 5.06e-13 | 69.7 |
| MS.gene048707.t1 | MTR_5g016350 | 29.630 | 162 | 90 | 6 | 13 | 158 | 412 | 565 | 1.33e-12 | 68.2 |
| MS.gene048707.t1 | MTR_5g016350 | 23.795 | 332 | 194 | 10 | 17 | 293 | 167 | 494 | 6.79e-13 | 69.3 |
| MS.gene048707.t1 | MTR_5g016350 | 29.630 | 162 | 90 | 6 | 13 | 158 | 348 | 501 | 2.72e-12 | 67.4 |
| MS.gene048707.t1 | MTR_4g119620 | 24.696 | 247 | 164 | 6 | 13 | 244 | 72 | 311 | 9.89e-13 | 67.8 |
| MS.gene048707.t1 | MTR_3g449990 | 25.263 | 285 | 191 | 9 | 17 | 281 | 268 | 550 | 3.70e-12 | 67.0 |
| MS.gene048707.t1 | MTR_6g004040 | 26.693 | 251 | 142 | 10 | 13 | 237 | 5 | 239 | 5.00e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048707.t1 | AT5G56130 | 80.635 | 315 | 45 | 5 | 1 | 299 | 1 | 315 | 0.0 | 522 |
| MS.gene048707.t1 | AT5G25150 | 25.600 | 250 | 124 | 8 | 15 | 244 | 456 | 663 | 8.51e-15 | 75.1 |
| MS.gene048707.t1 | AT5G25150 | 27.586 | 203 | 101 | 7 | 15 | 213 | 456 | 616 | 1.17e-13 | 71.6 |
| MS.gene048707.t1 | AT5G67320 | 31.013 | 158 | 93 | 5 | 12 | 157 | 441 | 594 | 5.69e-13 | 69.7 |
| MS.gene048707.t1 | AT5G67320 | 24.611 | 321 | 184 | 8 | 9 | 282 | 255 | 564 | 1.81e-12 | 68.2 |
| MS.gene048707.t1 | AT2G43770 | 24.161 | 298 | 195 | 11 | 16 | 287 | 50 | 342 | 9.70e-12 | 65.1 |
| MS.gene048707.t1 | AT3G49660 | 23.622 | 254 | 172 | 7 | 13 | 251 | 65 | 311 | 2.83e-11 | 63.5 |
| MS.gene048707.t1 | AT5G23430 | 24.364 | 275 | 148 | 9 | 13 | 269 | 5 | 237 | 4.38e-11 | 63.9 |
| MS.gene048707.t1 | AT5G23430 | 24.364 | 275 | 148 | 9 | 13 | 269 | 5 | 237 | 4.38e-11 | 63.9 |
| MS.gene048707.t1 | AT5G23430 | 24.364 | 275 | 148 | 9 | 13 | 269 | 5 | 237 | 4.54e-11 | 63.9 |
| MS.gene048707.t1 | AT5G08390 | 25.820 | 244 | 129 | 8 | 13 | 246 | 5 | 206 | 5.86e-11 | 63.5 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCAAGTACATTGTATTT+AGG | 0.212077 | 3.2:+60990370 | None:intergenic |
| TTGATCAAACTGCTCGAATT+TGG | 0.221602 | 3.2:-60997435 | MS.gene048707:CDS |
| ACAATTAGTTTCAATCATAC+TGG | 0.283022 | 3.2:-60991003 | MS.gene048707:CDS |
| TGCAATAAGATCGGGATGTT+TGG | 0.289165 | 3.2:+60996350 | None:intergenic |
| GCAATAAGATCGGGATGTTT+GGG | 0.297004 | 3.2:+60996351 | None:intergenic |
| GTGACAAGACTGTTCGTCTA+TGG | 0.297252 | 3.2:-60996317 | MS.gene048707:CDS |
| CGATCTTATTGCAACTGCTT+CGG | 0.300112 | 3.2:-60996340 | MS.gene048707:CDS |
| CTCATGCAGCTGTAGGTAAT+AGG | 0.305067 | 3.2:-60996036 | MS.gene048707:CDS |
| TGTCAGCTTGTGGGTTATTT+CGG | 0.322981 | 3.2:-60991182 | MS.gene048707:CDS |
| GATCTTATTGCAACTGCTTC+GGG | 0.329060 | 3.2:-60996339 | MS.gene048707:CDS |
| TTCTTTCTAACAACAGGAAA+TGG | 0.391405 | 3.2:-60991599 | MS.gene048707:intron |
| CTGATTCCCTTGTCAGCTTG+TGG | 0.395348 | 3.2:-60991192 | MS.gene048707:CDS |
| GCTCAATAAATGAGATTGCT+TGG | 0.402499 | 3.2:-60991640 | MS.gene048707:intron |
| TGATTCCCTTGTCAGCTTGT+GGG | 0.409663 | 3.2:-60991191 | MS.gene048707:CDS |
| CAGTACATCATATTCCTTGT+AGG | 0.413376 | 3.2:-60990420 | MS.gene048707:CDS |
| CTTCACAGCAGAGAGTATTC+TGG | 0.419755 | 3.2:-60997678 | MS.gene048707:CDS |
| ATTGGCACAAAGCTTGCTTC+TGG | 0.427408 | 3.2:-60997463 | MS.gene048707:CDS |
| TGCATTGCAATTGACCCAAC+AGG | 0.430132 | 3.2:-60991389 | MS.gene048707:CDS |
| AAAAGGTGCATTCAGTGGCT+TGG | 0.441542 | 3.2:-60997492 | MS.gene048707:intron |
| GGTCAAAGATATTGAATTGA+AGG | 0.446383 | 3.2:-60996409 | MS.gene048707:CDS |
| AGTTAACAATACTTGATGTT+CGG | 0.447811 | 3.2:-60995781 | MS.gene048707:CDS |
| GAGATGTTCTTTCTAACAAC+AGG | 0.457171 | 3.2:-60991605 | MS.gene048707:CDS |
| ATGAGTACCATCAGGTTTGT+AGG | 0.457397 | 3.2:+60996054 | None:intergenic |
| ATTCCACTCAACACTATTGA+TGG | 0.466131 | 3.2:+60990393 | None:intergenic |
| CAATTAGTTTCAATCATACT+GGG | 0.469646 | 3.2:-60991002 | MS.gene048707:CDS |
| TATGATTGAAACTAATTGTC+CGG | 0.470565 | 3.2:+60991007 | None:intergenic |
| TGTTTCTCAGATGGCCTGTC+CGG | 0.480041 | 3.2:-60991026 | MS.gene048707:intron |
| CTATTGATGGCAGCCCTACA+AGG | 0.486597 | 3.2:+60990406 | None:intergenic |
| AGGTGGAAAATGCTCACAAC+AGG | 0.499837 | 3.2:-60996107 | MS.gene048707:CDS |
| CGAAGCAGTTGCAATAAGAT+CGG | 0.502914 | 3.2:+60996341 | None:intergenic |
| TCATGCAGCTGTAGGTAATA+GGG | 0.503464 | 3.2:-60996035 | MS.gene048707:intron |
| CACAACAGGCAGAACTTAGT+GGG | 0.503581 | 3.2:-60996093 | MS.gene048707:CDS |
| GTATCAAGAGGTCGAAGAGA+TGG | 0.505442 | 3.2:+60991440 | None:intergenic |
| TAGTGTGGATCAGCTATGTT+GGG | 0.512894 | 3.2:-60996376 | MS.gene048707:CDS |
| TGACAAGACTGTTCGTCTAT+GGG | 0.513043 | 3.2:-60996316 | MS.gene048707:CDS |
| AACTTACTCGAGCTTTGTAA+AGG | 0.513452 | 3.2:+60991141 | None:intergenic |
| AAGAAGCATACCTTCCTGTT+GGG | 0.521567 | 3.2:+60991375 | None:intergenic |
| TCAGTGGCTTGGAATTGCAT+TGG | 0.521845 | 3.2:-60997481 | MS.gene048707:CDS |
| TCACAACAGGCAGAACTTAG+TGG | 0.530916 | 3.2:-60996094 | MS.gene048707:CDS |
| AAACGAGAATTGTTCCTCCA+TGG | 0.533256 | 3.2:+60997707 | None:intergenic |
| GAAGCAGTTGCAATAAGATC+GGG | 0.535149 | 3.2:+60996342 | None:intergenic |
| TTGAACTTACGCCTATGCAT+TGG | 0.535702 | 3.2:+60995749 | None:intergenic |
| TACAATGTACTTGCATATGC+CGG | 0.539937 | 3.2:-60990364 | MS.gene048707:CDS |
| AGTACATCATATTCCTTGTA+GGG | 0.547208 | 3.2:-60990419 | MS.gene048707:CDS |
| CAAGAAGCATACCTTCCTGT+TGG | 0.550714 | 3.2:+60991374 | None:intergenic |
| GTCAAAGATATTGAATTGAA+GGG | 0.552936 | 3.2:-60996408 | MS.gene048707:CDS |
| TACTCTCATGGCTCATACAG+CGG | 0.555050 | 3.2:-60991420 | MS.gene048707:CDS |
| ACTCTCATGGCTCATACAGC+GGG | 0.555763 | 3.2:-60991419 | MS.gene048707:CDS |
| ATAGTGTGGATCAGCTATGT+TGG | 0.561550 | 3.2:-60996377 | MS.gene048707:CDS |
| AAATAACCCACAAGCTGACA+AGG | 0.562424 | 3.2:+60991185 | None:intergenic |
| TCGACCTCTTGATACTCTCA+TGG | 0.565472 | 3.2:-60991432 | MS.gene048707:CDS |
| TAGGCGTAAGTTCAACTATG+AGG | 0.584012 | 3.2:-60995741 | MS.gene048707:intron |
| GGAAATTCAAACCAATGCAT+AGG | 0.586683 | 3.2:-60995760 | MS.gene048707:CDS |
| ACAGCTGCATGAGTACCATC+AGG | 0.587950 | 3.2:+60996046 | None:intergenic |
| TTGAAACTAATTGTCCGGAC+AGG | 0.589710 | 3.2:+60991012 | None:intergenic |
| GTATTCTGGTCACGAAAAGA+AGG | 0.591063 | 3.2:-60997664 | MS.gene048707:intron |
| AAGGTTACTACATGGGCATG+TGG | 0.595342 | 3.2:+60997403 | None:intergenic |
| GTTTCAAAAGGTGCATTCAG+TGG | 0.599434 | 3.2:-60997497 | MS.gene048707:intron |
| TTTCGGAGATGCTTTGTGTG+CGG | 0.599990 | 3.2:-60991165 | MS.gene048707:CDS |
| GAGATTGCTTGGAACATGAC+TGG | 0.603670 | 3.2:-60991629 | MS.gene048707:CDS |
| GAAGGGTCACACCGATAGTG+TGG | 0.604201 | 3.2:-60996391 | MS.gene048707:CDS |
| AACATCACCTACAAACCTGA+TGG | 0.607731 | 3.2:-60996061 | MS.gene048707:CDS |
| GATGGTACTCATGCAGCTGT+AGG | 0.608815 | 3.2:-60996043 | MS.gene048707:CDS |
| AGGTTACTACATGGGCATGT+GGG | 0.614543 | 3.2:+60997404 | None:intergenic |
| CAATAAGATCGGGATGTTTG+GGG | 0.614895 | 3.2:+60996352 | None:intergenic |
| CTGCCATCAATAGTGTTGAG+TGG | 0.622139 | 3.2:-60990396 | MS.gene048707:CDS |
| ACATAGCTGATCCACACTAT+CGG | 0.628331 | 3.2:+60996380 | None:intergenic |
| AACAAGTATCAGGCTGACGA+AGG | 0.633197 | 3.2:-60990331 | MS.gene048707:intron |
| TTGCAATTGACCCAACAGGA+AGG | 0.660962 | 3.2:-60991385 | MS.gene048707:intron |
| AATAACCCACAAGCTGACAA+GGG | 0.681570 | 3.2:+60991186 | None:intergenic |
| AATTAGTTTCAATCATACTG+GGG | 0.695286 | 3.2:-60991001 | MS.gene048707:CDS |
| TGAGCCATGAGAGTATCAAG+AGG | 0.696186 | 3.2:+60991428 | None:intergenic |
| ACAACAGGCAGAACTTAGTG+GGG | 0.714393 | 3.2:-60996092 | MS.gene048707:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 60990210 | 60997727 | 60990210 | ID=MS.gene048707 |
| chr3.2 | mRNA | 60990210 | 60997727 | 60990210 | ID=MS.gene048707.t1;Parent=MS.gene048707 |
| chr3.2 | exon | 60997665 | 60997727 | 60997665 | ID=MS.gene048707.t1.exon1;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60997665 | 60997727 | 60997665 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60997414 | 60997509 | 60997414 | ID=MS.gene048707.t1.exon2;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60997414 | 60997509 | 60997414 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60996307 | 60996436 | 60996307 | ID=MS.gene048707.t1.exon3;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60996307 | 60996436 | 60996307 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60996036 | 60996127 | 60996036 | ID=MS.gene048707.t1.exon4;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60996036 | 60996127 | 60996036 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60995742 | 60995810 | 60995742 | ID=MS.gene048707.t1.exon5;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60995742 | 60995810 | 60995742 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60991600 | 60991648 | 60991600 | ID=MS.gene048707.t1.exon6;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60991600 | 60991648 | 60991600 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60991386 | 60991473 | 60991386 | ID=MS.gene048707.t1.exon7;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60991386 | 60991473 | 60991386 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60991148 | 60991234 | 60991148 | ID=MS.gene048707.t1.exon8;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60991148 | 60991234 | 60991148 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60990956 | 60991038 | 60990956 | ID=MS.gene048707.t1.exon9;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60990956 | 60991038 | 60990956 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60990332 | 60990439 | 60990332 | ID=MS.gene048707.t1.exon10;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60990332 | 60990439 | 60990332 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| chr3.2 | exon | 60990210 | 60990244 | 60990210 | ID=MS.gene048707.t1.exon11;Parent=MS.gene048707.t1 |
| chr3.2 | CDS | 60990210 | 60990244 | 60990210 | ID=cds.MS.gene048707.t1;Parent=MS.gene048707.t1 |
| Gene Sequence |
| Protein sequence |
