AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049480.t1 MTR_3g466110 91.160 181 16 0 1 181 275 455 1.17e-102 305
MS.gene049480.t1 MTR_3g466040 81.868 182 32 1 1 181 275 456 3.40e-90 274
MS.gene049480.t1 MTR_8g071920 40.212 189 102 5 1 181 349 534 6.02e-32 122
MS.gene049480.t1 MTR_5g026350 40.719 167 92 4 17 181 330 491 2.41e-30 117
MS.gene049480.t1 MTR_7g083660 35.610 205 106 6 3 182 283 486 1.36e-28 112
MS.gene049480.t1 MTR_2g450710 31.088 193 127 4 2 193 307 494 4.49e-16 76.3
MS.gene049480.t1 MTR_4g073690 28.144 167 119 1 3 168 293 459 1.96e-15 74.3
MS.gene049480.t1 MTR_4g116210 32.824 131 88 0 38 168 349 479 5.11e-14 70.5
MS.gene049480.t1 MTR_5g022380 29.927 137 96 0 30 166 309 445 4.24e-13 67.8
MS.gene049480.t1 MTR_5g022380 29.927 137 96 0 30 166 199 335 7.39e-13 66.6
MS.gene049480.t1 MTR_7g029015 28.467 137 98 0 30 166 346 482 2.08e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049480.t1 AT2G30300 52.866 157 73 1 26 181 281 437 1.93e-48 165
MS.gene049480.t1 AT4G19450 38.830 188 102 5 4 181 309 493 9.26e-31 118
MS.gene049480.t1 AT5G45275 40.571 175 94 5 16 181 321 494 1.86e-29 114
MS.gene049480.t1 AT3G01630 36.313 179 104 4 10 181 304 479 1.48e-27 109
MS.gene049480.t1 AT3G01630 36.313 179 104 4 10 181 322 497 1.70e-27 109
MS.gene049480.t1 AT1G31470 34.239 184 115 4 2 181 317 498 1.48e-26 106
MS.gene049480.t1 AT4G19450 43.810 105 48 3 4 99 309 411 8.88e-19 84.0
MS.gene049480.t1 AT1G80530 29.050 179 125 2 3 180 306 483 1.68e-15 74.7
MS.gene049480.t1 AT4G34950 27.322 183 130 1 2 181 307 489 4.16e-15 73.6
MS.gene049480.t1 AT2G16660 30.496 141 98 0 41 181 328 468 6.07e-15 73.2
MS.gene049480.t1 AT1G74780 28.205 156 107 2 12 165 296 448 3.73e-11 62.0

Find 37 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGAGAAGAATAAATTTC+TGG 0.186844 3.2:+56770195 MS.gene049480:CDS
GTGTCTTTGTCTTCTGCTTT+TGG 0.192683 3.2:+56770323 MS.gene049480:CDS
TTGTTGTCTTGGAACTTTAT+TGG 0.202596 3.2:+56771444 MS.gene049480:CDS
TTTGGTGTTTCTTAACAATT+TGG 0.204315 3.2:+56770256 MS.gene049480:CDS
TACATAAGCACTGCAGTAAT+TGG 0.247602 3.2:+56771196 MS.gene049480:CDS
TCCACAACAACTGAGCTATT+TGG 0.249847 3.2:+56771253 MS.gene049480:CDS
CTGTTCTGCTACTTCATCTT+TGG 0.259954 3.2:+56770301 MS.gene049480:CDS
ATTGGTGTTATGGAAGAAAT+TGG 0.266375 3.2:+56770161 MS.gene049480:CDS
ACGTCTCATTCCATCTCTTT+TGG 0.289040 3.2:+56770355 MS.gene049480:CDS
AATTCAAGAGATTGGTGTTA+TGG 0.341430 3.2:+56770151 MS.gene049480:CDS
TTGGTGTTTCTTAACAATTT+GGG 0.387056 3.2:+56770257 MS.gene049480:CDS
TACTGCAGTGCTTATGTAAA+GGG 0.396066 3.2:-56771191 None:intergenic
CATAGAGAATGAAGTGAAAG+AGG 0.417366 3.2:+56770112 MS.gene049480:CDS
AGTTGTGAAATTCAAGAGAT+TGG 0.423500 3.2:+56770143 MS.gene049480:CDS
GCAGTAATTGGAGTTTCTAC+TGG 0.428724 3.2:+56771208 MS.gene049480:CDS
TAAAGTTCCAAGACAACAAA+AGG 0.451891 3.2:-56771440 None:intergenic
AAGAAGAATGCAAATGTTGT+TGG 0.467873 3.2:-56771145 None:intergenic
TGCCACTATAGTAGCAGTTA+TGG 0.477489 3.2:+56771120 MS.gene049480:CDS
TTCACTATTATTCTCCTCCA+TGG 0.479663 3.2:-56770084 None:intergenic
GCACTTATTTACCGTGGTGA+AGG 0.504829 3.2:+56771355 MS.gene049480:CDS
TTACTGCAGTGCTTATGTAA+AGG 0.505843 3.2:-56771192 None:intergenic
GCCAAATAGCTCAGTTGTTG+TGG 0.519172 3.2:-56771254 None:intergenic
CGTGGTGAAGGGAATGAACA+TGG 0.521353 3.2:+56771367 MS.gene049480:CDS
CACTTATTTACCGTGGTGAA+GGG 0.524996 3.2:+56771356 MS.gene049480:CDS
ATAGAGAATGAAGTGAAAGA+GGG 0.533673 3.2:+56770113 MS.gene049480:CDS
TGCCATAACTGCTACTATAG+TGG 0.544260 3.2:-56771122 None:intergenic
TAGAAGTAGTCCAAAAGAGA+TGG 0.550728 3.2:-56770365 None:intergenic
ATAACTGCTACTATAGTGGC+AGG 0.578194 3.2:-56771118 None:intergenic
GAATGAACATGGGAAATGCA+TGG 0.579212 3.2:+56771378 MS.gene049480:CDS
AATGAACATGGGAAATGCAT+GGG 0.586706 3.2:+56771379 MS.gene049480:CDS
ACATGGGAAATGCATGGGCA+TGG 0.611694 3.2:+56771384 MS.gene049480:CDS
CAGTGCTTATGTAAAGGGCA+AGG 0.629081 3.2:-56771186 None:intergenic
GGACAAATAGCTGAATCAAG+AGG 0.632165 3.2:+56770278 MS.gene049480:CDS
AGTTGTTGTGGAAATAGCAG+TGG 0.639364 3.2:-56771242 None:intergenic
CATGTTCATTCCCTTCACCA+CGG 0.670222 3.2:-56771366 None:intergenic
TCAGCTGCACTTATTTACCG+TGG 0.674454 3.2:+56771349 MS.gene049480:intron
GTGGTGAAGGGAATGAACAT+GGG 0.684893 3.2:+56771368 MS.gene049480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTATCTTGAAAATAT+AGG - chr3.2:56770780-56770799 None:intergenic 10.0%
!!! AATAATAATTTTAATTTCAG+GGG + chr3.2:56771076-56771095 MS.gene049480:intron 10.0%
!!! TATTTCTTTGTATATTTTTT+TGG + chr3.2:56770221-56770240 MS.gene049480:CDS 10.0%
!!! TTCAAGATAATTTTTATTTA+AGG + chr3.2:56770785-56770804 MS.gene049480:intron 10.0%
!! AAATTGTAAAAATTTAGTGT+CGG - chr3.2:56770589-56770608 None:intergenic 15.0%
!! AATATAAATAAAGAATCATG+TGG - chr3.2:56770426-56770445 None:intergenic 15.0%
!! AGTTATTAAGCAACAAAATT+AGG + chr3.2:56770703-56770722 MS.gene049480:intron 20.0%
!! ATTTAATGCAATAATGACAA+TGG + chr3.2:56770897-56770916 MS.gene049480:intron 20.0%
!! CAAAATATTGACACTATATA+CGG - chr3.2:56770626-56770645 None:intergenic 20.0%
!! CTAGTAACATTGAAAATATA+GGG - chr3.2:56771030-56771049 None:intergenic 20.0%
!!! AATATCACTGCTTTTTTTAA+AGG + chr3.2:56770652-56770671 MS.gene049480:intron 20.0%
!!! ATAGTAACACTTTTATCATT+TGG + chr3.2:56771413-56771432 MS.gene049480:CDS 20.0%
!!! GTTATGCTACATTTTAAAAA+GGG - chr3.2:56770847-56770866 None:intergenic 20.0%
!!! TAGTAACACTTTTATCATTT+GGG + chr3.2:56771414-56771433 MS.gene049480:CDS 20.0%
!!! TGTTATGCTACATTTTAAAA+AGG - chr3.2:56770848-56770867 None:intergenic 20.0%
! GCTAGTAACATTGAAAATAT+AGG - chr3.2:56771031-56771050 None:intergenic 25.0%
! TGTAAATCATAATGTAGTTG+TGG + chr3.2:56771291-56771310 MS.gene049480:intron 25.0%
! TGTTGAGAAGAATAAATTTC+TGG + chr3.2:56770195-56770214 MS.gene049480:CDS 25.0%
!! AGTAACACTTTTATCATTTG+GGG + chr3.2:56771415-56771434 MS.gene049480:CDS 25.0%
!! GAAAATTTTCGTTGCAATTT+AGG - chr3.2:56770531-56770550 None:intergenic 25.0%
!! TTGGTGTTTCTTAACAATTT+GGG + chr3.2:56770257-56770276 MS.gene049480:CDS 25.0%
!!! ATAAATGCCATGATTTTAGT+TGG - chr3.2:56770871-56770890 None:intergenic 25.0%
!!! GAAGAGGCTTTTAATTTTTT+TGG - chr3.2:56770960-56770979 None:intergenic 25.0%
!!! TATTTTTTTGGTGCAACTAT+TGG + chr3.2:56770233-56770252 MS.gene049480:CDS 25.0%
!!! TTTGGTGTTTCTTAACAATT+TGG + chr3.2:56770256-56770275 MS.gene049480:CDS 25.0%
AAGAAGAATGCAAATGTTGT+TGG - chr3.2:56771148-56771167 None:intergenic 30.0%
ACTCTGATGCAACTAATTTA+TGG + chr3.2:56770473-56770492 MS.gene049480:intron 30.0%
AGTTGTGAAATTCAAGAGAT+TGG + chr3.2:56770143-56770162 MS.gene049480:CDS 30.0%
ATAGAGAATGAAGTGAAAGA+GGG + chr3.2:56770113-56770132 MS.gene049480:CDS 30.0%
CATAACACCAACTAAAATCA+TGG + chr3.2:56770861-56770880 MS.gene049480:intron 30.0%
GACTACTTCTATAGGTAATA+TGG + chr3.2:56770377-56770396 MS.gene049480:intron 30.0%
TAAAGTTCCAAGACAACAAA+AGG - chr3.2:56771443-56771462 None:intergenic 30.0%
TTGTTGTCTTGGAACTTTAT+TGG + chr3.2:56771444-56771463 MS.gene049480:CDS 30.0%
! AATAATGACAATGGTGATGT+TGG + chr3.2:56770906-56770925 MS.gene049480:intron 30.0%
!! AATTCAAGAGATTGGTGTTA+TGG + chr3.2:56770151-56770170 MS.gene049480:CDS 30.0%
!! ATTGGTGTTATGGAAGAAAT+TGG + chr3.2:56770161-56770180 MS.gene049480:CDS 30.0%
!! TCTTCTGCTTTTGGATTTTT+TGG + chr3.2:56770332-56770351 MS.gene049480:CDS 30.0%
AATGAACATGGGAAATGCAT+GGG + chr3.2:56771379-56771398 MS.gene049480:CDS 35.0%
AGAATGCTAACTAGTGTTCA+GGG + chr3.2:56770675-56770694 MS.gene049480:intron 35.0%
AGCTGAGTAACCAAACAAAA+AGG - chr3.2:56771335-56771354 None:intergenic 35.0%
AGTTGTGGCAAATATTCCAA+TGG + chr3.2:56771306-56771325 MS.gene049480:intron 35.0%
ATTCCTTAACTAGTGCTTCT+GGG + chr3.2:56770808-56770827 MS.gene049480:intron 35.0%
CATAGAGAATGAAGTGAAAG+AGG + chr3.2:56770112-56770131 MS.gene049480:CDS 35.0%
GTTGTGGCAAATATTCCAAT+GGG + chr3.2:56771307-56771326 MS.gene049480:intron 35.0%
TACTGCAGTGCTTATGTAAA+GGG - chr3.2:56771194-56771213 None:intergenic 35.0%
TAGAAGTAGTCCAAAAGAGA+TGG - chr3.2:56770368-56770387 None:intergenic 35.0%
TTACTGCAGTGCTTATGTAA+AGG - chr3.2:56771195-56771214 None:intergenic 35.0%
! CTCTTTTGGACTACTTCTAT+AGG + chr3.2:56770369-56770388 MS.gene049480:CDS 35.0%
! TACATAAGCACTGCAGTAAT+TGG + chr3.2:56771196-56771215 MS.gene049480:CDS 35.0%
!! TTTTGGTGCAACTATTGGTT+TGG + chr3.2:56770238-56770257 MS.gene049480:CDS 35.0%
AGCCAAGTCAATAAGAGAAG+AGG - chr3.2:56770976-56770995 None:intergenic 40.0%
AGTTGTTGTGGAAATAGCAG+TGG - chr3.2:56771245-56771264 None:intergenic 40.0%
ATAACTGCTACTATAGTGGC+AGG - chr3.2:56771121-56771140 None:intergenic 40.0%
CAATTTAGGCAGCAACTTCA+AGG - chr3.2:56770517-56770536 None:intergenic 40.0%
CACTTATTTACCGTGGTGAA+GGG + chr3.2:56771356-56771375 MS.gene049480:CDS 40.0%
CCAAACAAAAAGGATCCCAT+TGG - chr3.2:56771325-56771344 None:intergenic 40.0%
GAATGAACATGGGAAATGCA+TGG + chr3.2:56771378-56771397 MS.gene049480:CDS 40.0%
GAATGCTAACTAGTGTTCAG+GGG + chr3.2:56770676-56770695 MS.gene049480:intron 40.0%
GAGAATGCTAACTAGTGTTC+AGG + chr3.2:56770674-56770693 MS.gene049480:intron 40.0%
GATTCCTTAACTAGTGCTTC+TGG + chr3.2:56770807-56770826 MS.gene049480:intron 40.0%
GCAGTAATTGGAGTTTCTAC+TGG + chr3.2:56771208-56771227 MS.gene049480:CDS 40.0%
GGACAAATAGCTGAATCAAG+AGG + chr3.2:56770278-56770297 MS.gene049480:CDS 40.0%
TCCACAACAACTGAGCTATT+TGG + chr3.2:56771253-56771272 MS.gene049480:CDS 40.0%
TGCCACTATAGTAGCAGTTA+TGG + chr3.2:56771120-56771139 MS.gene049480:CDS 40.0%
TGCCATAACTGCTACTATAG+TGG - chr3.2:56771125-56771144 None:intergenic 40.0%
! ACGTCTCATTCCATCTCTTT+TGG + chr3.2:56770355-56770374 MS.gene049480:CDS 40.0%
! AGCCTCTTCTCTTATTGACT+TGG + chr3.2:56770971-56770990 MS.gene049480:intron 40.0%
! CCAATGGGATCCTTTTTGTT+TGG + chr3.2:56771322-56771341 MS.gene049480:intron 40.0%
! CTGTTCTGCTACTTCATCTT+TGG + chr3.2:56770301-56770320 MS.gene049480:CDS 40.0%
! GTGTCTTTGTCTTCTGCTTT+TGG + chr3.2:56770323-56770342 MS.gene049480:CDS 40.0%
CAGTGCTTATGTAAAGGGCA+AGG - chr3.2:56771189-56771208 None:intergenic 45.0%
CATGTTCATTCCCTTCACCA+CGG - chr3.2:56771369-56771388 None:intergenic 45.0%
GCACTTATTTACCGTGGTGA+AGG + chr3.2:56771355-56771374 MS.gene049480:CDS 45.0%
GCCAAATAGCTCAGTTGTTG+TGG - chr3.2:56771257-56771276 None:intergenic 45.0%
GTGGTGAAGGGAATGAACAT+GGG + chr3.2:56771368-56771387 MS.gene049480:CDS 45.0%
TCAGCTGCACTTATTTACCG+TGG + chr3.2:56771349-56771368 MS.gene049480:intron 45.0%
!! TGGGGTTCCTTTTGTTGTCT+TGG + chr3.2:56771433-56771452 MS.gene049480:CDS 45.0%
!! TGTCCCAGAAGCACTAGTTA+AGG - chr3.2:56770814-56770833 None:intergenic 45.0%
!!! TAATAATAATTTTAATTTCA+GGG + chr3.2:56771075-56771094 MS.gene049480:intron 5.0%
!!! TTAATAATAATTTTAATTTC+AGG + chr3.2:56771074-56771093 MS.gene049480:intron 5.0%
ACATGGGAAATGCATGGGCA+TGG + chr3.2:56771384-56771403 MS.gene049480:CDS 50.0%
CGTGGTGAAGGGAATGAACA+TGG + chr3.2:56771367-56771386 MS.gene049480:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 56770086 56771472 56770086 ID=MS.gene049480
chr3.2 mRNA 56770086 56771472 56770086 ID=MS.gene049480.t1;Parent=MS.gene049480
chr3.2 exon 56770086 56770390 56770086 ID=MS.gene049480.t1.exon1;Parent=MS.gene049480.t1
chr3.2 CDS 56770086 56770390 56770086 ID=cds.MS.gene049480.t1;Parent=MS.gene049480.t1
chr3.2 exon 56771096 56771291 56771096 ID=MS.gene049480.t1.exon2;Parent=MS.gene049480.t1
chr3.2 CDS 56771096 56771291 56771096 ID=cds.MS.gene049480.t1;Parent=MS.gene049480.t1
chr3.2 exon 56771353 56771472 56771353 ID=MS.gene049480.t1.exon3;Parent=MS.gene049480.t1
chr3.2 CDS 56771353 56771472 56771353 ID=cds.MS.gene049480.t1;Parent=MS.gene049480.t1
Gene Sequence

>MS.gene049480

ATGGAGGAGAATAATAGTGAAGAGAGCATAGAGAATGAAGTGAAAGAGGGTGAAGATAGTTGTGAAATTCAAGAGATTGGTGTTATGGAAGAAATTGGAGTGAAGTTAATGTTGAGAAGAATAAATTTCTGGTTATATTTCTTTGTATATTTTTTTGGTGCAACTATTGGTTTGGTGTTTCTTAACAATTTGGGACAAATAGCTGAATCAAGAGGCTGTTCTGCTACTTCATCTTTGGTGTCTTTGTCTTCTGCTTTTGGATTTTTTGGACGTCTCATTCCATCTCTTTTGGACTACTTCTATAGGTAATATGGAACAATTTCTATGCATATTTATGCCACATGATTCTTTATTTATATTCTAGAGAATTGCAGTTAAATGTGTCTGACTCTGATGCAACTAATTTATGGTTGCATTGTCGTAGAAAACCTTGAAGTTGCTGCCTAAATTGCAACGAAAATTTTCTAAAAAAAAATGTCGTTTTGACACAAGTTCGACATCCGACACTAAATTTTTACAATTTTGATCTTTTTGTGTCCGTATATAGTGTCAATATTTTGTGTCTAAATATCACTGCTTTTTTTAAAGGAGAATGCTAACTAGTGTTCAGGGGCATTAGTTATTAAGCAACAAAATTAGGAAAGTTCTTTATAAAAAGACAGTGTAATTATTACTTTATCTAAGTAAAAAACCTATATTTTCAAGATAATTTTTATTTAAGGATTCCTTAACTAGTGCTTCTGGGACAGTTAGTGAGACCCTTTTTAAAATGTAGCATAACACCAACTAAAATCATGGCATTTATGCTATCATTTAATGCAATAATGACAATGGTGATGTTGGTTGATCTACATTGATGAAGACAAACAAACCAAAAAAATTAAAAGCCTCTTCTCTTATTGACTTGGCTAGTAGCTACTACAAAAAGTACTATTTTTTTTCCCTATATTTTCAATGTTACTAGCTACTACATAGTACTATATACTAATTAATAATAATTTTAATTTCAGGGGAAAACATACAATTTCAAGACCTGCCACTATAGTAGCAGTTATGGCACCAACAACATTTGCATTCTTCTTACTTCTCAACAAAACTGACCTTGCCCTTTACATAAGCACTGCAGTAATTGGAGTTTCTACTGGAGCAATCACTTCCACTGCTATTTCCACAACAACTGAGCTATTTGGCACAAAGAATTTTTCTGTAAATCATAATGTAGTTGTGGCAAATATTCCAATGGGATCCTTTTTGTTTGGTTACTCAGCTGCACTTATTTACCGTGGTGAAGGGAATGAACATGGGAAATGCATGGGCATGGAATGTTATAGTAACACTTTTATCATTTGGGGTTCCTTTTGTTGTCTTGGAACTTTATTGGCTTTGA

Protein sequence

>MS.gene049480.t1

MEENNSEESIENEVKEGEDSCEIQEIGVMEEIGVKLMLRRINFWLYFFVYFFGATIGLVFLNNLGQIAESRGCSATSSLVSLSSAFGFFGRLIPSLLDYFYRGKHTISRPATIVAVMAPTTFAFFLLLNKTDLALYISTAVIGVSTGAITSTAISTTTELFGTKNFSLHLFTVVKGMNMGNAWAWNVIVTLLSFGVPFVVLELYWL