AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97081


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97081.t1 AT1G72030 52.747 182 80 3 14 190 60 240 3.39e-53 171

Find 33 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAGTACCTTTACATTTC+AGG 0.191164 4.4:-69925590 MS.gene97081:CDS
TAGCTGTCTCCAATGCTTTC+AGG 0.230533 4.4:-69925565 MS.gene97081:intron
TTTGTTCTTTCAATTCTTTA+AGG 0.237732 4.4:-69926471 MS.gene97081:intron
AAGAGCTTATGAAGAAGATT+TGG 0.266902 4.4:-69925206 MS.gene97081:CDS
AATCTTCTTCATAAGCTCTT+AGG 0.289976 4.4:+69925209 None:intergenic
AAATAGGCTGAAGTGCTTTC+AGG 0.312360 4.4:-69925943 MS.gene97081:intron
AAACTCATTACCCCACAAAT+TGG 0.313360 4.4:+69925237 None:intergenic
ATTGTTTAGGTATGCTTGTT+TGG 0.344763 4.4:-69925714 MS.gene97081:intron
TGAAAATATGAAGCAAAAGT+TGG 0.383459 4.4:-69926615 MS.gene97081:CDS
TAACAACAAATGTACCTATC+AGG 0.398497 4.4:+69925892 None:intergenic
AGAGCTTATGAAGAAGATTT+GGG 0.419407 4.4:-69925205 MS.gene97081:CDS
GTGAATATGGATGGAGGATA+AGG 0.442867 4.4:-69926580 MS.gene97081:CDS
AGGTACTCTTGTGCATCAGC+AGG 0.454568 4.4:+69925602 None:intergenic
TTAAATACTCACCTCCTGAT+AGG 0.463612 4.4:-69925906 MS.gene97081:intron
GTGACATAATATCCAATTTG+TGG 0.473968 4.4:-69925249 MS.gene97081:CDS
GATTCATCAAAGGAACTTGT+AGG 0.482799 4.4:-69925674 MS.gene97081:CDS
TGACATAATATCCAATTTGT+GGG 0.486196 4.4:-69925248 MS.gene97081:CDS
AAATCATTGAAAAGAGCTAC+AGG 0.509660 4.4:+69926491 None:intergenic
CGAACATTGTACACAAATGC+TGG 0.511040 4.4:-69925178 MS.gene97081:CDS
ACAATCAAAGAGAGAGCTCA+AGG 0.518793 4.4:-69924753 MS.gene97081:CDS
CAGCTATTCCTGAAATGTAA+AGG 0.520945 4.4:+69925582 None:intergenic
GGAGATGTTCAAGCACATCT+TGG 0.525750 4.4:+69925624 None:intergenic
GTTTAGGTATGCTTGTTTGG+TGG 0.529125 4.4:-69925711 MS.gene97081:intron
CCAACCCAATTACTTGTCCA+TGG 0.539516 4.4:+69925130 None:intergenic
GAATGATGAAGATTCATCAA+AGG 0.579526 4.4:-69925684 MS.gene97081:CDS
GTGGATCTTTAGACACAAGT+TGG 0.596499 4.4:+69925152 None:intergenic
TGGTGTGTGAATATGGATGG+AGG 0.600815 4.4:-69926586 MS.gene97081:CDS
GGTACTCTTGTGCATCAGCA+GGG 0.603876 4.4:+69925603 None:intergenic
CAATCAAAGAGAGAGCTCAA+GGG 0.619765 4.4:-69924752 MS.gene97081:CDS
ACCCAATTACTTGTCCATGG+TGG 0.661718 4.4:+69925133 None:intergenic
CAACAAATGTACCTATCAGG+AGG 0.675327 4.4:+69925895 None:intergenic
GACATAATATCCAATTTGTG+GGG 0.680586 4.4:-69925247 MS.gene97081:CDS
TTGTGTCTAAAGATCCACCA+TGG 0.708310 4.4:-69925147 MS.gene97081:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATATTCTACTTAATT+AGG + chr4.4:69926310-69926329 None:intergenic 10.0%
!!! GTCAAAATTTATTATTTTAA+AGG + chr4.4:69925341-69925360 None:intergenic 10.0%
!! ATCATTTATCAATATGAAAT+TGG - chr4.4:69925526-69925545 MS.gene97081:intron 15.0%
!! TAATGTATATACTATTGTTT+AGG - chr4.4:69925644-69925663 MS.gene97081:CDS 15.0%
!!! ATATTCAAGGATTTTTAAAT+AGG - chr4.4:69925864-69925883 MS.gene97081:intron 15.0%
!! AACATTAAACAACCATTTAA+AGG + chr4.4:69925391-69925410 None:intergenic 20.0%
!! ATTTAAAGGCAAATAAAATG+TGG + chr4.4:69925377-69925396 None:intergenic 20.0%
!! TTCAGACAAAATATTAGAAA+TGG - chr4.4:69926335-69926354 MS.gene97081:intron 20.0%
!! TTTGTTCTTTCAATTCTTTA+AGG - chr4.4:69924900-69924919 MS.gene97081:intron 20.0%
!!! ACATTTTATTTGCCTTTAAA+TGG - chr4.4:69925376-69925395 MS.gene97081:intron 20.0%
! TAATGAGAGTAACTAATTGA+TGG - chr4.4:69926059-69926078 MS.gene97081:intron 25.0%
! TGAAAATATGAAGCAAAAGT+TGG - chr4.4:69924756-69924775 MS.gene97081:CDS 25.0%
! TGACATAATATCCAATTTGT+GGG - chr4.4:69926123-69926142 MS.gene97081:intron 25.0%
! TTCTCTACTTATTGTCTTAA+AGG + chr4.4:69925153-69925172 None:intergenic 25.0%
! TTTGATTGATCTACTGTATA+AGG - chr4.4:69926556-69926575 MS.gene97081:CDS 25.0%
!! AATAGTAACAAGATTTTGAG+TGG - chr4.4:69925013-69925032 MS.gene97081:intron 25.0%
!!! TTACATCTCTGAAATATTCA+AGG - chr4.4:69925851-69925870 MS.gene97081:intron 25.0%
AAAGGCAAATAAAATGTGGT+AGG + chr4.4:69925373-69925392 None:intergenic 30.0%
AAATCATTGAAAAGAGCTAC+AGG + chr4.4:69924883-69924902 None:intergenic 30.0%
AAGACAATAAGTAGAGAATC+TGG - chr4.4:69925155-69925174 MS.gene97081:CDS 30.0%
AAGAGCTTATGAAGAAGATT+TGG - chr4.4:69926165-69926184 MS.gene97081:intron 30.0%
AATCTTCTTCATAAGCTCTT+AGG + chr4.4:69926165-69926184 None:intergenic 30.0%
AGAGCTTATGAAGAAGATTT+GGG - chr4.4:69926166-69926185 MS.gene97081:intron 30.0%
ATTCTAACCACTAGACAATT+TGG + chr4.4:69925126-69925145 None:intergenic 30.0%
ATTGTTTAGGTATGCTTGTT+TGG - chr4.4:69925657-69925676 MS.gene97081:CDS 30.0%
ATTTCACCATAAAGAGAGTT+TGG + chr4.4:69925064-69925083 None:intergenic 30.0%
GAAATTGCTTCTCAAATTGT+AGG - chr4.4:69925079-69925098 MS.gene97081:intron 30.0%
GAATGATGAAGATTCATCAA+AGG - chr4.4:69925687-69925706 MS.gene97081:CDS 30.0%
GAATGCAGATGAGATAAAAA+TGG - chr4.4:69924825-69924844 MS.gene97081:intron 30.0%
GACATAATATCCAATTTGTG+GGG - chr4.4:69926124-69926143 MS.gene97081:intron 30.0%
GTGACATAATATCCAATTTG+TGG - chr4.4:69926122-69926141 MS.gene97081:intron 30.0%
TAACAACAAATGTACCTATC+AGG + chr4.4:69925482-69925501 None:intergenic 30.0%
TGATGACACTGAAATAGTAT+TGG + chr4.4:69926441-69926460 None:intergenic 30.0%
TTCTAACCACTAGACAATTT+GGG + chr4.4:69925125-69925144 None:intergenic 30.0%
TTTGATCCAAACTCTCTTTA+TGG - chr4.4:69925055-69925074 MS.gene97081:intron 30.0%
! AGTTTTCTTTTTGTGTGTTC+AGG + chr4.4:69925920-69925939 None:intergenic 30.0%
! GTTTTCTTTTTGTGTGTTCA+GGG + chr4.4:69925919-69925938 None:intergenic 30.0%
!! TTTTAATCACCTGAAAGCAT+TGG + chr4.4:69925818-69925837 None:intergenic 30.0%
!!! GAAGCAAAAGTTGGATTTTT+TGG - chr4.4:69924765-69924784 MS.gene97081:CDS 30.0%
!!! GATTTTTTGGTGTGTGAATA+TGG - chr4.4:69924778-69924797 MS.gene97081:CDS 30.0%
AAAATGTGGTAGGTGCTTAA+TGG + chr4.4:69925363-69925382 None:intergenic 35.0%
AAACTCATTACCCCACAAAT+TGG + chr4.4:69926137-69926156 None:intergenic 35.0%
ATCAAAGCAGATGTTGAACT+AGG + chr4.4:69926542-69926561 None:intergenic 35.0%
CAGCTATTCCTGAAATGTAA+AGG + chr4.4:69925792-69925811 None:intergenic 35.0%
GATTCATCAAAGGAACTTGT+AGG - chr4.4:69925697-69925716 MS.gene97081:CDS 35.0%
GCTAAATCACTAGTTGCTAA+TGG + chr4.4:69925991-69926010 None:intergenic 35.0%
TATCCATTCTTGACCCAAAT+AGG - chr4.4:69925412-69925431 MS.gene97081:intron 35.0%
TATTTCAGTGTCATCAATCG+TGG - chr4.4:69926445-69926464 MS.gene97081:intron 35.0%
TTAAATACTCACCTCCTGAT+AGG - chr4.4:69925465-69925484 MS.gene97081:intron 35.0%
TTGCTTCTCAAATTGTAGGA+TGG - chr4.4:69925083-69925102 MS.gene97081:intron 35.0%
! TAAGTAGAGAATCTGGAGTT+CGG - chr4.4:69925162-69925181 MS.gene97081:CDS 35.0%
! TCTTTTCCCAAATTGTCTAG+TGG - chr4.4:69925116-69925135 MS.gene97081:intron 35.0%
!! CAAGAGTACCTTTACATTTC+AGG - chr4.4:69925781-69925800 MS.gene97081:intron 35.0%
!! TTTTGGTGTGTGAATATGGA+TGG - chr4.4:69924782-69924801 MS.gene97081:CDS 35.0%
ACAATCAAAGAGAGAGCTCA+AGG - chr4.4:69926618-69926637 MS.gene97081:CDS 40.0%
AGAAGGAAAAGACGATTGCA+AGG + chr4.4:69926513-69926532 None:intergenic 40.0%
ATCCACCATGGACAAGTAAT+TGG - chr4.4:69926236-69926255 MS.gene97081:intron 40.0%
CAACAAATGTACCTATCAGG+AGG + chr4.4:69925479-69925498 None:intergenic 40.0%
CAATCAAAGAGAGAGCTCAA+GGG - chr4.4:69926619-69926638 MS.gene97081:CDS 40.0%
CGAACATTGTACACAAATGC+TGG - chr4.4:69926193-69926212 MS.gene97081:intron 40.0%
GTGAATATGGATGGAGGATA+AGG - chr4.4:69924791-69924810 MS.gene97081:CDS 40.0%
GTGGATCTTTAGACACAAGT+TGG + chr4.4:69926222-69926241 None:intergenic 40.0%
GTTTAGGTATGCTTGTTTGG+TGG - chr4.4:69925660-69925679 MS.gene97081:CDS 40.0%
TCCACCATGGACAAGTAATT+GGG - chr4.4:69926237-69926256 MS.gene97081:intron 40.0%
TTGTGTCTAAAGATCCACCA+TGG - chr4.4:69926224-69926243 MS.gene97081:intron 40.0%
! AAATAGGCTGAAGTGCTTTC+AGG - chr4.4:69925428-69925447 MS.gene97081:intron 40.0%
! GAGTAACTAATTGATGGCAC+AGG - chr4.4:69926065-69926084 MS.gene97081:intron 40.0%
! GTTGAACTAGGTTTTGCAGA+AGG + chr4.4:69926530-69926549 None:intergenic 40.0%
! TAACTAATTGATGGCACAGG+AGG - chr4.4:69926068-69926087 MS.gene97081:intron 40.0%
!! GAAAGCACTTCAGCCTATTT+GGG + chr4.4:69925428-69925447 None:intergenic 40.0%
!! TGAAAGCACTTCAGCCTATT+TGG + chr4.4:69925429-69925448 None:intergenic 40.0%
ACCCAATTACTTGTCCATGG+TGG + chr4.4:69926241-69926260 None:intergenic 45.0%
CAGCCTATTTGGGTCAAGAA+TGG + chr4.4:69925418-69925437 None:intergenic 45.0%
CCAACCCAATTACTTGTCCA+TGG + chr4.4:69926244-69926263 None:intergenic 45.0%
CCATGGACAAGTAATTGGGT+TGG - chr4.4:69926241-69926260 MS.gene97081:intron 45.0%
GGAGATGTTCAAGCACATCT+TGG + chr4.4:69925750-69925769 None:intergenic 45.0%
TAGCTGTCTCCAATGCTTTC+AGG - chr4.4:69925806-69925825 MS.gene97081:intron 45.0%
TGGTGTGTGAATATGGATGG+AGG - chr4.4:69924785-69924804 MS.gene97081:CDS 45.0%
!!! TTAAAATTAATAATTAGTTT+AGG + chr4.4:69925555-69925574 None:intergenic 5.0%
AGGTACTCTTGTGCATCAGC+AGG + chr4.4:69925772-69925791 None:intergenic 50.0%
GGTACTCTTGTGCATCAGCA+GGG + chr4.4:69925771-69925790 None:intergenic 50.0%
! CTAATTGATGGCACAGGAGG+AGG - chr4.4:69926071-69926090 MS.gene97081:intron 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 69924733 69926660 69924733 ID=MS.gene97081
chr4.4 mRNA 69924733 69926660 69924733 ID=MS.gene97081.t1;Parent=MS.gene97081
chr4.4 exon 69926472 69926660 69926472 ID=MS.gene97081.t1.exon1;Parent=MS.gene97081.t1
chr4.4 CDS 69926472 69926660 69926472 ID=cds.MS.gene97081.t1;Parent=MS.gene97081.t1
chr4.4 exon 69925907 69925959 69925907 ID=MS.gene97081.t1.exon2;Parent=MS.gene97081.t1
chr4.4 CDS 69925907 69925959 69925907 ID=cds.MS.gene97081.t1;Parent=MS.gene97081.t1
chr4.4 exon 69925566 69925727 69925566 ID=MS.gene97081.t1.exon3;Parent=MS.gene97081.t1
chr4.4 CDS 69925566 69925727 69925566 ID=cds.MS.gene97081.t1;Parent=MS.gene97081.t1
chr4.4 exon 69925143 69925306 69925143 ID=MS.gene97081.t1.exon4;Parent=MS.gene97081.t1
chr4.4 CDS 69925143 69925306 69925143 ID=cds.MS.gene97081.t1;Parent=MS.gene97081.t1
chr4.4 exon 69924733 69924815 69924733 ID=MS.gene97081.t1.exon5;Parent=MS.gene97081.t1
chr4.4 CDS 69924733 69924815 69924733 ID=cds.MS.gene97081.t1;Parent=MS.gene97081.t1
Gene Sequence

>MS.gene97081

ATGTTGGAAATTGAGAATGATAGTGAAAATATGAAGCAAAAGTTGGATTTTTTGGTGTGTGAATATGGATGGAGGATAAGGAGATTAATTGAGAATGCAGATGAGATAAAAATGGCTGCTCAAGTTCAAGCTGAAGCTTTTCATGATCCTGTAGCTCTTTTCAATGATTTGTTCTTTCAATTCTTTAAGGTTCTGCTTCTCTCTTTTTATTAGTGTTTATATTTTATCTTCATTTTTATGTACTTTTCATATGCTATTTTATTTCTTAAGATTTTATTTAAATAGTAACAAGATTTTGAGTGGAACTTGAGTGTAAAAACTGTTTGATCCAAACTCTCTTTATGGTGAAATTGCTTCTCAAATTGTAGGATGGTAAATTTAATTCTTTTCCCAAATTGTCTAGTGGTTAGAATTTCTCCTTTAAGACAATAAGTAGAGAATCTGGAGTTCGGACTCTGACTTTCACATATATAATACAATGTCGTATTAACTGAGCTATGTTCAGCGAGACAAATTTAGTCTCTTTAGATATAAGAAATTTTAAAATAATTTTTAAAAATTGAAAATGATTAATCTAATAATACTTTTAGATTAGTCAAATTAGTCCTTTAAAATAATAAATTTTGACCATTAAGCACCTACCACATTTTATTTGCCTTTAAATGGTTGTTTAATGTTTTATCCATTCTTGACCCAAATAGGCTGAAGTGCTTTCAGGACTGCTTTACAAGCTTAAATACTCACCTCCTGATAGGTACATTTGTTGTTATTTCTAAACAAGTTTATGTAGATTATCATTTATCAATATGAAATTGGTGACCTAAACTAATTATTAATTTTAATTAAAAAAAAAACTCATGATAAATCTGAGCATGAGATAATAAATTGATCTTGTGTAAAACATTAGCAACTAATGTATATACTATTGTTTAGGTATGCTTGTTTGGTGGCTGAGAATGATGAAGATTCATCAAAGGAACTTGTAGGTGTTATAGATGTTACTGTAATGAGAGACCAAGATGTGCTTGAACATCTCCCTGCTGATGCACAAGAGTACCTTTACATTTCAGGAATAGCTGTCTCCAATGCTTTCAGGTGATTAAAAGATTTAATTACTATTACATCTCTGAAATATTCAAGGATTTTTAAATAGGTCATTGAAATAAAATATTTGTAACTTAGTCCCTGAACACACAAAAAGAAAACTGTTATATAGCGACTGATATTAGATATCAAAATATTAAATCGATCGCTACCATTAGCAACTAGTGATTTAGCGACATTTTACACATTGATATTTTTCATGTGATGTGTGAAACTATATGTTAATGAGAGTAACTAATTGATGGCACAGGAGGAGGAAAATTGCAACTGCTATGTTAAAAGCATGTGACATAATATCCAATTTGTGGGGTAATGAGTTTCTTGTCCTAAGAGCTTATGAAGAAGATTTGGGTGCTCGAACATTGTACACAAATGCTGGCTACCAACTTGTGTCTAAAGATCCACCATGGACAAGTAATTGGGTTGGAAGAAAATGTCGTGTTCTTATGATCAAAAGAATTAGTTTATTGCCTAATTAAGTAGAATATTAATTCAAAATTCAGACAAAATATTAGAAATGGAAAATGTATTCTATGTACATATCATATGTCAGCATATTTATTTAATTATTTCTCATTGATTACACACAAAACAAGTTTATCCAATACTATTTCAGTGTCATCAATCGTGGATAGCGAAAAAAAGCATGTTGTTTAAATCGATGAATATGATACCTTGCAATCGTCTTTTCCTTCTGCAAAACCTAGTTCAACATCTGCTTTGATTGATCTACTGTATAAGGTGACACGAGAGAAGTGCAATCTGAGATCGATAGAGAAGAACAATCAAAGAGAGAGCTCAAGGGAGTGAGAGAGAACCGTTGA

Protein sequence

>MS.gene97081.t1

MLEIENDSENMKQKLDFLVCEYGWRIRRLIENADEIKMAAQVQAEAFHDPVALFNDLFFQFFKAEVLSGLLYKLKYSPPDRYACLVAENDEDSSKELVGVIDVTVMRDQDVLEHLPADAQEYLYISGIAVSNAFRRRKIATAMLKACDIISNLWGNEFLVLRAYEEDLGARTLYTNAGYQLVSKDPPWTSDTREVQSEIDREEQSKRELKGVRENR