AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62091


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62091.t1 MTR_4g131400 97.024 168 5 0 1 168 1 168 1.26e-121 340
MS.gene62091.t1 MTR_4g131500 81.761 159 29 0 10 168 5 163 3.70e-96 275
MS.gene62091.t1 MTR_4g131390 77.439 164 37 0 5 168 2 165 1.03e-94 272
MS.gene62091.t1 MTR_5g082980 64.103 39 14 0 130 168 72 110 5.02e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62091.t1 AT5G58070 76.970 165 38 0 4 168 2 166 1.14e-92 267

Find 51 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGCTTGGCTTCATCACTTT+TGG 0.137021 8.2:-3724904 None:intergenic
TTCCTGCTGTTGGAGATTAC+TGG 0.269337 8.2:+3724964 MS.gene62091:CDS
CTTATAAGCACTTCCTTCTA+TGG 0.269670 8.2:-3724874 None:intergenic
GGCCTACCAATAAATATCTA+TGG 0.270272 8.2:+3725030 MS.gene62091:CDS
CATAGAAGGAAGTGCTTATA+AGG 0.280018 8.2:+3724875 MS.gene62091:CDS
GCTTACCCATAGATATTTAT+TGG 0.283517 8.2:-3725036 None:intergenic
TCTCCAACAGCAGGAATAAT+TGG 0.308299 8.2:-3724957 None:intergenic
TGTAGATTGCTTCATCCAAA+TGG 0.401693 8.2:-3725506 None:intergenic
ACTATGCAGGCAACCCCATT+TGG 0.413202 8.2:+3725491 MS.gene62091:CDS
TTCATGTTCTCAACGAGACT+TGG 0.427700 8.2:+3724832 MS.gene62091:CDS
GTAGATTGCTTCATCCAAAT+GGG 0.436253 8.2:-3725505 None:intergenic
GGTAAAAGAACCTCCATAGA+AGG 0.443815 8.2:+3724861 MS.gene62091:CDS
GGTTGTGAAGGGTGTAGACT+TGG 0.444182 8.2:+3724701 MS.gene62091:CDS
ATAGAATTTAACCTTGAGCT+TGG 0.456530 8.2:-3724919 None:intergenic
GAAGGACAAGGAGGTTGTGA+AGG 0.458131 8.2:+3724689 MS.gene62091:CDS
GATTATCAATATGCTCTCAT+TGG 0.460982 8.2:+3725005 MS.gene62091:CDS
TTGCCAATTATTCCTGCTGT+TGG 0.461126 8.2:+3724954 MS.gene62091:CDS
GCAGGAATAATTGGCAAGAA+TGG 0.465330 8.2:-3724948 None:intergenic
CCTTAGCTTTCTCAACAAGC+TGG 0.479647 8.2:-3725530 None:intergenic
GTTGAGAAAGCTAAGGAAGA+AGG 0.488479 8.2:+3725537 MS.gene62091:CDS
TGTAGACTTGGAGAGGTACA+TGG 0.499697 8.2:+3724713 MS.gene62091:CDS
CATATTGATAATCTTGATCA+AGG 0.510400 8.2:-3724995 None:intergenic
ATCCAGTAATCTCCAACAGC+AGG 0.514048 8.2:-3724966 None:intergenic
TATCAATATGCTCTCATTGG+TGG 0.520368 8.2:+3725008 MS.gene62091:CDS
ATCAATATGCTCTCATTGGT+GGG 0.522349 8.2:+3725009 MS.gene62091:CDS
AAGGACAAGGAGGTTGTGAA+GGG 0.527129 8.2:+3724690 MS.gene62091:CDS
ACCCATAGATATTTATTGGT+AGG 0.528773 8.2:-3725032 None:intergenic
GCCTACCAATAAATATCTAT+GGG 0.548969 8.2:+3725031 MS.gene62091:CDS
TTGGTCCCTAGATACTATGC+AGG 0.558593 8.2:+3725478 MS.gene62091:intron
TGAGGACCTTAATCTTACTC+AGG 0.562281 8.2:-3725606 None:intergenic
ACATACACTCTCAACAGTGA+TGG 0.564431 8.2:+3724804 MS.gene62091:CDS
ATAAGCACTTCCTTCTATGG+AGG 0.564915 8.2:-3724871 None:intergenic
AACCTCCTTGTCCTTCCCTA+CGG 0.565364 8.2:-3724682 None:intergenic
CCTTAATCTTACTCAGGTGG+AGG 0.569308 8.2:-3725600 None:intergenic
ATCACTGTTGAGAGTGTATG+TGG 0.583825 8.2:-3724802 None:intergenic
GGAATAATTGGCAAGAATGG+AGG 0.591527 8.2:-3724945 None:intergenic
AATGGGAAACACCGTAGGGA+AGG 0.597163 8.2:+3724671 None:intergenic
AAGTGATGAAGCCAAGCTCA+AGG 0.599556 8.2:+3724908 MS.gene62091:CDS
CATACACTCTCAACAGTGAT+GGG 0.600831 8.2:+3724805 MS.gene62091:CDS
GGACCTTAATCTTACTCAGG+TGG 0.603353 8.2:-3725603 None:intergenic
TAGATTGCTTCATCCAAATG+GGG 0.620741 8.2:-3725504 None:intergenic
ACTTGGAGAGGTACATGGGT+AGG 0.635238 8.2:+3724718 MS.gene62091:CDS
CCAGCTTGTTGAGAAAGCTA+AGG 0.636813 8.2:+3725530 MS.gene62091:CDS
GTTCTCAACGAGACTTGGAA+TGG 0.638379 8.2:+3724837 MS.gene62091:CDS
CTCAACGAGACTTGGAATGG+TGG 0.641563 8.2:+3724840 MS.gene62091:CDS
GTAGACTTGGAGAGGTACAT+GGG 0.653419 8.2:+3724714 MS.gene62091:CDS
TGAAGGGTGTAGACTTGGAG+AGG 0.655962 8.2:+3724706 MS.gene62091:CDS
TGGAGAGGTACATGGGTAGG+TGG 0.656685 8.2:+3724721 MS.gene62091:CDS
TCAGGTGGAGGATCACTCTG+TGG 0.704206 8.2:-3725588 None:intergenic
CACCGTAGGGAAGGACAAGG+AGG 0.708944 8.2:+3724680 MS.gene62091:CDS
AAACACCGTAGGGAAGGACA+AGG 0.745653 8.2:+3724677 MS.gene62091:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTAAAAATTGTTTTATTTA+AGG + chr8.2:3725381-3725400 MS.gene62091:intron 10.0%
!!! TTGCTTTTTCTTGTTAAATT+AGG + chr8.2:3725075-3725094 MS.gene62091:intron 20.0%
! AAATAATCCCAAGTTATTGT+AGG - chr8.2:3725459-3725478 None:intergenic 25.0%
! ATCAAATATAATCACATGTG+TGG - chr8.2:3725124-3725143 None:intergenic 25.0%
! TCAAATATAATCACATGTGT+GGG - chr8.2:3725123-3725142 None:intergenic 25.0%
!! ACAATAACTTGGGATTATTT+TGG + chr8.2:3725459-3725478 MS.gene62091:intron 25.0%
!! AGAGAAAAAAAGATTAGTTG+TGG + chr8.2:3725233-3725252 MS.gene62091:intron 25.0%
!! CATATTGATAATCTTGATCA+AGG - chr8.2:3724998-3725017 None:intergenic 25.0%
!! TTGACTTCTAAGAAAAATTG+TGG + chr8.2:3725258-3725277 MS.gene62091:intron 25.0%
AAAAAGCAACGTTAGAGATA+GGG - chr8.2:3725064-3725083 None:intergenic 30.0%
ACCCATAGATATTTATTGGT+AGG - chr8.2:3725035-3725054 None:intergenic 30.0%
ATAGAATTTAACCTTGAGCT+TGG - chr8.2:3724922-3724941 None:intergenic 30.0%
GATTATCAATATGCTCTCAT+TGG + chr8.2:3725005-3725024 MS.gene62091:CDS 30.0%
GCCTACCAATAAATATCTAT+GGG + chr8.2:3725031-3725050 MS.gene62091:CDS 30.0%
GCTTACCCATAGATATTTAT+TGG - chr8.2:3725039-3725058 None:intergenic 30.0%
TAGATTGTCTTGGTTTATTG+TGG - chr8.2:3725286-3725305 None:intergenic 30.0%
AATCCCAAGTTATTGTAGGA+AGG - chr8.2:3725455-3725474 None:intergenic 35.0%
AGGAACATTAGTATACACTC+AGG - chr8.2:3725435-3725454 None:intergenic 35.0%
ATCAATATGCTCTCATTGGT+GGG + chr8.2:3725009-3725028 MS.gene62091:CDS 35.0%
CAATCATAGCTAGATTGTCT+TGG - chr8.2:3725296-3725315 None:intergenic 35.0%
CTTATAAGCACTTCCTTCTA+TGG - chr8.2:3724877-3724896 None:intergenic 35.0%
GAAAAAGCAACGTTAGAGAT+AGG - chr8.2:3725065-3725084 None:intergenic 35.0%
GGCCTACCAATAAATATCTA+TGG + chr8.2:3725030-3725049 MS.gene62091:CDS 35.0%
GTAGATTGCTTCATCCAAAT+GGG - chr8.2:3725508-3725527 None:intergenic 35.0%
GTTCCTTCCTACAATAACTT+GGG + chr8.2:3725449-3725468 MS.gene62091:intron 35.0%
TAGATTGCTTCATCCAAATG+GGG - chr8.2:3725507-3725526 None:intergenic 35.0%
TATCAATATGCTCTCATTGG+TGG + chr8.2:3725008-3725027 MS.gene62091:CDS 35.0%
TCATTCTTTCAGCCCAAAAA+TGG + chr8.2:3724765-3724784 MS.gene62091:CDS 35.0%
TGTAGATTGCTTCATCCAAA+TGG - chr8.2:3725509-3725528 None:intergenic 35.0%
TGTTCCTTCCTACAATAACT+TGG + chr8.2:3725448-3725467 MS.gene62091:intron 35.0%
! TCTTGTGTTCTCTCCATTTT+TGG - chr8.2:3724781-3724800 None:intergenic 35.0%
! TTTTGGGCTGAAAGAATGAA+GGG - chr8.2:3724764-3724783 None:intergenic 35.0%
!! CATAGAAGGAAGTGCTTATA+AGG + chr8.2:3724875-3724894 MS.gene62091:CDS 35.0%
!! CTTGTGTTCTCTCCATTTTT+GGG - chr8.2:3724780-3724799 None:intergenic 35.0%
!! TTTTTGGGCTGAAAGAATGA+AGG - chr8.2:3724765-3724784 None:intergenic 35.0%
ACATACACTCTCAACAGTGA+TGG + chr8.2:3724804-3724823 MS.gene62091:CDS 40.0%
ATAAGCACTTCCTTCTATGG+AGG - chr8.2:3724874-3724893 None:intergenic 40.0%
ATGAAGCTATGACACAGACA+TGG - chr8.2:3725213-3725232 None:intergenic 40.0%
CATACACTCTCAACAGTGAT+GGG + chr8.2:3724805-3724824 MS.gene62091:CDS 40.0%
GCAGGAATAATTGGCAAGAA+TGG - chr8.2:3724951-3724970 None:intergenic 40.0%
GGAATAATTGGCAAGAATGG+AGG - chr8.2:3724948-3724967 None:intergenic 40.0%
GGTAAAAGAACCTCCATAGA+AGG + chr8.2:3724861-3724880 MS.gene62091:CDS 40.0%
GTTGAGAAAGCTAAGGAAGA+AGG + chr8.2:3725537-3725556 MS.gene62091:CDS 40.0%
TCTCCAACAGCAGGAATAAT+TGG - chr8.2:3724960-3724979 None:intergenic 40.0%
TTCATGTTCTCAACGAGACT+TGG + chr8.2:3724832-3724851 MS.gene62091:CDS 40.0%
TTGCCAATTATTCCTGCTGT+TGG + chr8.2:3724954-3724973 MS.gene62091:CDS 40.0%
! ATCACTGTTGAGAGTGTATG+TGG - chr8.2:3724805-3724824 None:intergenic 40.0%
!! AGCTTGGCTTCATCACTTTT+GGG - chr8.2:3724906-3724925 None:intergenic 40.0%
AAGGACAAGGAGGTTGTGAA+GGG + chr8.2:3724690-3724709 MS.gene62091:CDS 45.0%
AAGTGATGAAGCCAAGCTCA+AGG + chr8.2:3724908-3724927 MS.gene62091:CDS 45.0%
ATCCAGTAATCTCCAACAGC+AGG - chr8.2:3724969-3724988 None:intergenic 45.0%
CACATGTGTGGGATGTAACT+TGG - chr8.2:3725112-3725131 None:intergenic 45.0%
GGTTGCCTGCATAGTATCTA+GGG - chr8.2:3725486-3725505 None:intergenic 45.0%
GTTCTCAACGAGACTTGGAA+TGG + chr8.2:3724837-3724856 MS.gene62091:CDS 45.0%
TTCCTGCTGTTGGAGATTAC+TGG + chr8.2:3724964-3724983 MS.gene62091:CDS 45.0%
TTGGTCCCTAGATACTATGC+AGG + chr8.2:3725478-3725497 MS.gene62091:intron 45.0%
! CCTTAGCTTTCTCAACAAGC+TGG - chr8.2:3725533-3725552 None:intergenic 45.0%
! GAGCTTGGCTTCATCACTTT+TGG - chr8.2:3724907-3724926 None:intergenic 45.0%
! GTAGACTTGGAGAGGTACAT+GGG + chr8.2:3724714-3724733 MS.gene62091:CDS 45.0%
! TGTAGACTTGGAGAGGTACA+TGG + chr8.2:3724713-3724732 MS.gene62091:CDS 45.0%
!! CCAGCTTGTTGAGAAAGCTA+AGG + chr8.2:3725530-3725549 MS.gene62091:CDS 45.0%
!! GCTTGGCTTCATCACTTTTG+GGG - chr8.2:3724905-3724924 None:intergenic 45.0%
AAACACCGTAGGGAAGGACA+AGG + chr8.2:3724677-3724696 MS.gene62091:CDS 50.0%
AACCTCCTTGTCCTTCCCTA+CGG - chr8.2:3724685-3724704 None:intergenic 50.0%
ACTATGCAGGCAACCCCATT+TGG + chr8.2:3725491-3725510 MS.gene62091:CDS 50.0%
CTCAACGAGACTTGGAATGG+TGG + chr8.2:3724840-3724859 MS.gene62091:CDS 50.0%
GAAGGACAAGGAGGTTGTGA+AGG + chr8.2:3724689-3724708 MS.gene62091:CDS 50.0%
GGGTTGCCTGCATAGTATCT+AGG - chr8.2:3725487-3725506 None:intergenic 50.0%
! ACTTGGAGAGGTACATGGGT+AGG + chr8.2:3724718-3724737 MS.gene62091:CDS 50.0%
! GGTTGTGAAGGGTGTAGACT+TGG + chr8.2:3724701-3724720 MS.gene62091:CDS 50.0%
! TGAAGGGTGTAGACTTGGAG+AGG + chr8.2:3724706-3724725 MS.gene62091:CDS 50.0%
TCAGGTGGAGGATCACTCTG+TGG - chr8.2:3725591-3725610 None:intergenic 55.0%
! TGGAGAGGTACATGGGTAGG+TGG + chr8.2:3724721-3724740 MS.gene62091:CDS 55.0%
CACCGTAGGGAAGGACAAGG+AGG + chr8.2:3724680-3724699 MS.gene62091:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 3724672 3725614 3724672 ID=MS.gene62091
chr8.2 mRNA 3724672 3725614 3724672 ID=MS.gene62091.t1;Parent=MS.gene62091
chr8.2 exon 3724672 3725052 3724672 ID=MS.gene62091.t1.exon1;Parent=MS.gene62091.t1
chr8.2 CDS 3724672 3725052 3724672 ID=cds.MS.gene62091.t1;Parent=MS.gene62091.t1
chr8.2 exon 3725489 3725614 3725489 ID=MS.gene62091.t1.exon2;Parent=MS.gene62091.t1
chr8.2 CDS 3725489 3725614 3725489 ID=cds.MS.gene62091.t1;Parent=MS.gene62091.t1
Gene Sequence

>MS.gene62091

ATGGGAAACACCGTAGGGAAGGACAAGGAGGTTGTGAAGGGTGTAGACTTGGAGAGGTACATGGGTAGGTGGTATGAGATAGCTTCTTTCCCTTCATTCTTTCAGCCCAAAAATGGAGAGAACACAAGAGCCACATACACTCTCAACAGTGATGGGACTGTTCATGTTCTCAACGAGACTTGGAATGGTGGTAAAAGAACCTCCATAGAAGGAAGTGCTTATAAGGCTGACCCCAAAAGTGATGAAGCCAAGCTCAAGGTTAAATTCTATGTTCCTCCATTCTTGCCAATTATTCCTGCTGTTGGAGATTACTGGATTTTGTACCTTGATCAAGATTATCAATATGCTCTCATTGGTGGGCCTACCAATAAATATCTATGGGTAAGCAACCCTATCTCTAACGTTGCTTTTTCTTGTTAAATTAGGCATCATCGACACCAAGTTACATCCCACACATGTGATTATATTTGATTAATTCATTTTTAAAATTTATTATAGCATCTTTATGTTCAATGTCGTATATGTGTCTGTGTAGCATCCATGTCTGTGTCATAGCTTCATAGAGAAAAAAAGATTAGTTGTGGTTTTGACTTCTAAGAAAAATTGTGGAACCACAATAAACCAAGACAATCTAGCTATGATTGAACTAAATCATAGATATGAAAATGTTTAGCTAAAGACTAAGTTGTTAAAATTTATTTTTTACATGACTAAAAATTGTTTTATTTAAGGATTATTTTTTCAATCATCATGTCATGAGCCTGAGTGTATACTAATGTTCCTTCCTACAATAACTTGGGATTATTTTGGTCCCTAGATACTATGCAGGCAACCCCATTTGGATGAAGCAATCTACAACCAGCTTGTTGAGAAAGCTAAGGAAGAAGGTTATGATGTGAGTAAATTGCACAAGACTCCACAGAGTGATCCTCCACCTGAGTAA

Protein sequence

>MS.gene62091.t1

MGNTVGKDKEVVKGVDLERYMGRWYEIASFPSFFQPKNGENTRATYTLNSDGTVHVLNETWNGGKRTSIEGSAYKADPKSDEAKLKVKFYVPPFLPIIPAVGDYWILYLDQDYQYALIGGPTNKYLWILCRQPHLDEAIYNQLVEKAKEEGYDVSKLHKTPQSDPPPE