AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene42747


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42747.t1 MTR_8g046460 93.284 268 12 2 1 262 1 268 5.61e-178 490
MS.gene42747.t1 MTR_2g064370 70.522 268 72 4 1 262 1 267 3.79e-131 372
MS.gene42747.t1 MTR_8g028425 57.143 154 63 2 72 223 92 244 5.45e-65 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene42747.t1 AT1G16320 69.231 221 67 1 43 262 53 273 4.49e-117 336
MS.gene42747.t1 AT1G79510 60.714 280 87 5 1 262 1 275 1.99e-116 335
MS.gene42747.t1 AT1G79510 60.714 280 87 5 1 262 1 275 1.99e-116 335
MS.gene42747.t1 AT2G46220 57.325 157 64 2 69 223 81 236 2.50e-66 206

Find 57 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAAACCGGTTTCGGTTT+TGG 0.220818 8.4:-54349936 MS.gene42747:CDS
GACCCTTTGAACACATTTAC+TGG 0.240823 8.4:-54350220 MS.gene42747:CDS
AGAAGTACAAGTTGATCTTT+TGG 0.270946 8.4:-54350192 MS.gene42747:CDS
AGGTACTTCGCGGTATAAAT+TGG 0.272271 8.4:-54350023 MS.gene42747:CDS
ACCTTAATTATGATATTTAT+AGG 0.278702 8.4:-54350686 MS.gene42747:intron
ACCTATAAATATCATAATTA+AGG 0.297853 8.4:+54350685 None:intergenic
CCCCATGAAAATGTTGGATT+TGG 0.313331 8.4:+54349887 None:intergenic
TGGATTCTAGTTCATGTTCA+TGG 0.337564 8.4:-54349859 MS.gene42747:CDS
GAATAAAGAGTATTGAGAAA+TGG 0.346750 8.4:+54350878 None:intergenic
AATAAAGAGTATTGAGAAAT+GGG 0.349742 8.4:+54350879 None:intergenic
GTTTGGATGTATACAGAATA+TGG 0.351747 8.4:-54350129 MS.gene42747:CDS
TTGGTGTTTGAATCTTGATT+TGG 0.370847 8.4:+54350920 None:intergenic
TGTTTATCTTGAGTAGCTAA+TGG 0.394872 8.4:+54350798 None:intergenic
GAAGATGATGAAACCGGTTT+CGG 0.396965 8.4:-54349942 MS.gene42747:CDS
GTTGTTACCACCAGATGGTT+TGG 0.415329 8.4:-54349789 MS.gene42747:CDS
GGAACTTGAGGGGTGTGCCT+AGG 0.417152 8.4:-54350075 MS.gene42747:CDS
TTTGTTTCGTGAGATTGGTT+TGG 0.421815 8.4:-54350146 MS.gene42747:CDS
GAACTTGAGGGGTGTGCCTA+GGG 0.426698 8.4:-54350074 MS.gene42747:CDS
ACCAAATCCAAAACCGAAAC+CGG 0.442141 8.4:+54349929 None:intergenic
CAACCTGTGCAGTGAAACTT+TGG 0.444081 8.4:+54350835 None:intergenic
AATGTTGGATTTGGACTAGC+AGG 0.451399 8.4:+54349896 None:intergenic
TTTCACTGCACAGGTTGCTC+AGG 0.452221 8.4:-54350829 MS.gene42747:CDS
GGGGTGTGCCTAGGGTTCCA+TGG 0.454206 8.4:-54350066 MS.gene42747:CDS
GGGTGTGCCTAGGGTTCCAT+GGG 0.462556 8.4:-54350065 MS.gene42747:CDS
TTGAATCTTGATTTGGATTG+AGG 0.469278 8.4:+54350927 None:intergenic
GAACATGCAGCCAAACCATC+TGG 0.478212 8.4:+54349779 None:intergenic
CAGAGAATGTGATATTGATC+AGG 0.478427 8.4:-54350099 MS.gene42747:CDS
TACCAGTAAATGTGTTCAAA+GGG 0.481927 8.4:+54350218 None:intergenic
AGTCCAAAGTTTCACTGCAC+AGG 0.485891 8.4:-54350838 MS.gene42747:CDS
TCCACAGAAGATGATGAAAC+CGG 0.487224 8.4:-54349948 MS.gene42747:CDS
ATACCAGTAAATGTGTTCAA+AGG 0.496720 8.4:+54350217 None:intergenic
TAAACAGAATCAGCATCAAA+AGG 0.500362 8.4:-54350781 MS.gene42747:CDS
GAAGCTAAAGGTGAATTTCA+AGG 0.517611 8.4:-54350043 MS.gene42747:CDS
GTGAATTTCAAGGTACTTCG+CGG 0.529074 8.4:-54350033 MS.gene42747:CDS
ATCAATATCACATTCTCTGA+AGG 0.533171 8.4:+54350103 None:intergenic
AATTCACCTTTAGCTTCCCA+TGG 0.534577 8.4:+54350049 None:intergenic
TTAGCTTCCCATGGAACCCT+AGG 0.536034 8.4:+54350058 None:intergenic
TATTGATCAGGTGGAACTTG+AGG 0.540071 8.4:-54350087 MS.gene42747:CDS
AAAGGACCCCATGAAAATGT+TGG 0.541364 8.4:+54349881 None:intergenic
ATTGATCAGGTGGAACTTGA+GGG 0.551874 8.4:-54350086 MS.gene42747:CDS
ACCGGTTTCATCATCTTCTG+TGG 0.561359 8.4:+54349947 None:intergenic
AGGGTTCCATGGGAAGCTAA+AGG 0.578430 8.4:-54350055 MS.gene42747:CDS
GAAAGATTGAGATTGCAAAG+AGG 0.578460 8.4:+54350957 None:intergenic
AGGTCTTTAACAAAGATCAA+AGG 0.587544 8.4:+54350705 None:intergenic
AGACAGTTGTTACCACCAGA+TGG 0.589181 8.4:-54349794 MS.gene42747:CDS
GCAGTGAAACTTTGGACTGA+AGG 0.593412 8.4:+54350843 None:intergenic
AGAATGTGATATTGATCAGG+TGG 0.610163 8.4:-54350096 MS.gene42747:CDS
TCAAGCAGTTAAGCACACAT+TGG 0.624208 8.4:-54349825 MS.gene42747:CDS
CTTCGCGGTATAAATTGGAT+CGG 0.643822 8.4:-54350018 MS.gene42747:CDS
GAACATGAACTAGAATCCAA+AGG 0.647700 8.4:+54349863 None:intergenic
TGCTGTTAGAACCCTTCGTG+AGG 0.651000 8.4:-54350733 MS.gene42747:CDS
CGGTATAAATTGGATCGGAA+CGG 0.651485 8.4:-54350013 MS.gene42747:CDS
TTGATCAGGTGGAACTTGAG+GGG 0.654298 8.4:-54350085 MS.gene42747:CDS
CAAAGGCATATCCTCACGAA+GGG 0.657035 8.4:+54350722 None:intergenic
ATGTTGGATTTGGACTAGCA+GGG 0.662829 8.4:+54349897 None:intergenic
TCAAAGGCATATCCTCACGA+AGG 0.664210 8.4:+54350721 None:intergenic
CATGCAGCCAAACCATCTGG+TGG 0.695452 8.4:+54349782 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACCTATAAATATCATAATTA+AGG + chr8.4:54350059-54350078 None:intergenic 15.0%
!! ACCTTAATTATGATATTTAT+AGG - chr8.4:54350055-54350074 MS.gene42747:CDS 15.0%
!! GATGATGTAATTAATATTTA+TGG - chr8.4:54350204-54350223 MS.gene42747:CDS 15.0%
!!! AAAAATTGGATTTTTAATGT+TGG - chr8.4:54350165-54350184 MS.gene42747:CDS 15.0%
!!! AAAATTGGATTTTTAATGTT+GGG - chr8.4:54350166-54350185 MS.gene42747:CDS 15.0%
!!! ATAGTTAGAATTTTAATGTT+GGG - chr8.4:54350462-54350481 MS.gene42747:intron 15.0%
!!! TAAAAAAAGTTAATTTTGAG+TGG - chr8.4:54350407-54350426 MS.gene42747:intron 15.0%
!!! TATAGTTAGAATTTTAATGT+TGG - chr8.4:54350461-54350480 MS.gene42747:intron 15.0%
!!! TTGCTTTTCATATAAATATA+TGG - chr8.4:54350108-54350127 MS.gene42747:CDS 15.0%
!!! AATAAAGAGTATTGAGAAAT+GGG + chr8.4:54349865-54349884 None:intergenic 20.0%
!!! AATTTTGAGTGGAAAAAAAT+TGG - chr8.4:54350418-54350437 MS.gene42747:intron 20.0%
!!! ATTTCTAGGTGAAAAAAAAT+TGG - chr8.4:54350151-54350170 MS.gene42747:CDS 20.0%
!!! ATTTGAATTGTGTTATTTCT+AGG - chr8.4:54350137-54350156 MS.gene42747:CDS 20.0%
!!! ATTTTGAGTGGAAAAAAATT+GGG - chr8.4:54350419-54350438 MS.gene42747:intron 20.0%
!!! CTTTTTTTATACATAGAAAG+TGG + chr8.4:54350396-54350415 None:intergenic 20.0%
! ATTAATATTTATGGACCCTT+TGG - chr8.4:54350213-54350232 MS.gene42747:CDS 25.0%
! TTAATTCTGAGTAATCTGTA+AGG - chr8.4:54350269-54350288 MS.gene42747:intron 25.0%
!! TATAGGTAATTTTCTCATCT+GGG - chr8.4:54350072-54350091 MS.gene42747:CDS 25.0%
!! TTATAGGTAATTTTCTCATC+TGG - chr8.4:54350071-54350090 MS.gene42747:CDS 25.0%
!!! GAATAAAGAGTATTGAGAAA+TGG + chr8.4:54349866-54349885 None:intergenic 25.0%
!!! TGGGTTTGATAATTTTTTCA+GGG - chr8.4:54350481-54350500 MS.gene42747:intron 25.0%
!!! TTGGGTTTGATAATTTTTTC+AGG - chr8.4:54350480-54350499 MS.gene42747:intron 25.0%
AAATGTCGATATAGTTAGGT+CGG - chr8.4:54350307-54350326 MS.gene42747:intron 30.0%
AGGTCTTTAACAAAGATCAA+AGG + chr8.4:54350039-54350058 None:intergenic 30.0%
ATACCAGTAAATGTGTTCAA+AGG + chr8.4:54350527-54350546 None:intergenic 30.0%
ATCAATATCACATTCTCTGA+AGG + chr8.4:54350641-54350660 None:intergenic 30.0%
GTTTGGATGTATACAGAATA+TGG - chr8.4:54350612-54350631 MS.gene42747:intron 30.0%
TAAACAGAATCAGCATCAAA+AGG - chr8.4:54349960-54349979 MS.gene42747:CDS 30.0%
TACCAGTAAATGTGTTCAAA+GGG + chr8.4:54350526-54350545 None:intergenic 30.0%
TGGTAAATGTCGATATAGTT+AGG - chr8.4:54350303-54350322 MS.gene42747:intron 30.0%
TGTTTATCTTGAGTAGCTAA+TGG + chr8.4:54349946-54349965 None:intergenic 30.0%
! TCCAAATCCAACATTTTCAT+GGG - chr8.4:54350853-54350872 MS.gene42747:CDS 30.0%
! TGGATTTTTAATGTTGGGTT+TGG - chr8.4:54350171-54350190 MS.gene42747:CDS 30.0%
! TTGGTGTTTGAATCTTGATT+TGG + chr8.4:54349824-54349843 None:intergenic 30.0%
!! AGAATTTTGTTTCGTGAGAT+TGG - chr8.4:54350590-54350609 MS.gene42747:intron 30.0%
!! GATGAGTTTTATCTCAATCT+TGG - chr8.4:54349982-54350001 MS.gene42747:CDS 30.0%
!! TGTTGGGTTTGGTAATTTTA+AGG - chr8.4:54350182-54350201 MS.gene42747:CDS 30.0%
!! TTGAATCTTGATTTGGATTG+AGG + chr8.4:54349817-54349836 None:intergenic 30.0%
!!! AGAAGTACAAGTTGATCTTT+TGG - chr8.4:54350549-54350568 MS.gene42747:intron 30.0%
!!! GAAGTACAAGTTGATCTTTT+GGG - chr8.4:54350550-54350569 MS.gene42747:intron 30.0%
ACCAATATATGCACATCCAA+AGG + chr8.4:54350232-54350251 None:intergenic 35.0%
AGAATGTGATATTGATCAGG+TGG - chr8.4:54350645-54350664 MS.gene42747:intron 35.0%
CAGAGAATGTGATATTGATC+AGG - chr8.4:54350642-54350661 MS.gene42747:intron 35.0%
CCAATATATGCACATCCAAA+GGG + chr8.4:54350231-54350250 None:intergenic 35.0%
GAAAGATTGAGATTGCAAAG+AGG + chr8.4:54349787-54349806 None:intergenic 35.0%
GAACATGAACTAGAATCCAA+AGG + chr8.4:54350881-54350900 None:intergenic 35.0%
GAAGCTAAAGGTGAATTTCA+AGG - chr8.4:54350698-54350717 MS.gene42747:CDS 35.0%
! CAGGGATGATGTAACATTTT+TGG - chr8.4:54350499-54350518 MS.gene42747:intron 35.0%
! CCAAATCCAACATTTTCATG+GGG - chr8.4:54350854-54350873 MS.gene42747:CDS 35.0%
! GTCCAAATCCAACATTTTCA+TGG - chr8.4:54350852-54350871 MS.gene42747:CDS 35.0%
! TTTGTTTCGTGAGATTGGTT+TGG - chr8.4:54350595-54350614 MS.gene42747:intron 35.0%
!! TGGATTCTAGTTCATGTTCA+TGG - chr8.4:54350882-54350901 MS.gene42747:CDS 35.0%
!!! GTTTTGAAGAAGACAGTGTT+TGG + chr8.4:54349843-54349862 None:intergenic 35.0%
AAAGGACCCCATGAAAATGT+TGG + chr8.4:54350863-54350882 None:intergenic 40.0%
ACCAAATCCAAAACCGAAAC+CGG + chr8.4:54350815-54350834 None:intergenic 40.0%
AGGTACTTCGCGGTATAAAT+TGG - chr8.4:54350718-54350737 MS.gene42747:CDS 40.0%
ATTGATCAGGTGGAACTTGA+GGG - chr8.4:54350655-54350674 MS.gene42747:intron 40.0%
CCCCATGAAAATGTTGGATT+TGG + chr8.4:54350857-54350876 None:intergenic 40.0%
CCCTTTGGATGTGCATATAT+TGG - chr8.4:54350228-54350247 MS.gene42747:CDS 40.0%
CGGTATAAATTGGATCGGAA+CGG - chr8.4:54350728-54350747 MS.gene42747:CDS 40.0%
CTTCGCGGTATAAATTGGAT+CGG - chr8.4:54350723-54350742 MS.gene42747:CDS 40.0%
GACCCTTTGAACACATTTAC+TGG - chr8.4:54350521-54350540 MS.gene42747:intron 40.0%
TATTGATCAGGTGGAACTTG+AGG - chr8.4:54350654-54350673 MS.gene42747:intron 40.0%
TCAAGCAGTTAAGCACACAT+TGG - chr8.4:54350916-54350935 MS.gene42747:CDS 40.0%
TCCACAGAAGATGATGAAAC+CGG - chr8.4:54350793-54350812 MS.gene42747:CDS 40.0%
! AACATTTTCATGGGGTCCTT+TGG - chr8.4:54350862-54350881 MS.gene42747:CDS 40.0%
! AATTCACCTTTAGCTTCCCA+TGG + chr8.4:54350695-54350714 None:intergenic 40.0%
! GAAGATGATGAAACCGGTTT+CGG - chr8.4:54350799-54350818 MS.gene42747:CDS 40.0%
! GTGAATTTCAAGGTACTTCG+CGG - chr8.4:54350708-54350727 MS.gene42747:CDS 40.0%
!! AATGTTGGATTTGGACTAGC+AGG + chr8.4:54350848-54350867 None:intergenic 40.0%
!! AGTTGATCTTTTGGGCATTG+AGG - chr8.4:54350558-54350577 MS.gene42747:intron 40.0%
!! ATGTTGGATTTGGACTAGCA+GGG + chr8.4:54350847-54350866 None:intergenic 40.0%
!!! TTTTGGGCATTGAGGTTTCA+TGG - chr8.4:54350566-54350585 MS.gene42747:intron 40.0%
AGACAGTTGTTACCACCAGA+TGG - chr8.4:54350947-54350966 MS.gene42747:CDS 45.0%
AGTCCAAAGTTTCACTGCAC+AGG - chr8.4:54349903-54349922 MS.gene42747:CDS 45.0%
CAACCTGTGCAGTGAAACTT+TGG + chr8.4:54349909-54349928 None:intergenic 45.0%
CGACATTTACCAGTTGAGCT+AGG + chr8.4:54350295-54350314 None:intergenic 45.0%
GCAGTGAAACTTTGGACTGA+AGG + chr8.4:54349901-54349920 None:intergenic 45.0%
GTTGTTACCACCAGATGGTT+TGG - chr8.4:54350952-54350971 MS.gene42747:CDS 45.0%
TTGATCAGGTGGAACTTGAG+GGG - chr8.4:54350656-54350675 MS.gene42747:intron 45.0%
! ACCGGTTTCATCATCTTCTG+TGG + chr8.4:54350797-54350816 None:intergenic 45.0%
! CAAAGGCATATCCTCACGAA+GGG + chr8.4:54350022-54350041 None:intergenic 45.0%
! TCAAAGGCATATCCTCACGA+AGG + chr8.4:54350023-54350042 None:intergenic 45.0%
!! GATGAAACCGGTTTCGGTTT+TGG - chr8.4:54350805-54350824 MS.gene42747:CDS 45.0%
!!! ACCGGTTTCGGTTTTGGATT+TGG - chr8.4:54350811-54350830 MS.gene42747:CDS 45.0%
AGGGTTCCATGGGAAGCTAA+AGG - chr8.4:54350686-54350705 MS.gene42747:intron 50.0%
AGTCGTTCGAACCCAGGTTA+AGG + chr8.4:54350336-54350355 None:intergenic 50.0%
GAACATGCAGCCAAACCATC+TGG + chr8.4:54350965-54350984 None:intergenic 50.0%
TAGGTCGGACACCTTAACCT+GGG - chr8.4:54350322-54350341 MS.gene42747:intron 50.0%
TCTGTAAGGCCTAGCTCAAC+TGG - chr8.4:54350283-54350302 MS.gene42747:intron 50.0%
TGCTGTTAGAACCCTTCGTG+AGG - chr8.4:54350008-54350027 MS.gene42747:CDS 50.0%
TTAGCTTCCCATGGAACCCT+AGG + chr8.4:54350686-54350705 None:intergenic 50.0%
TTAGGTCGGACACCTTAACC+TGG - chr8.4:54350321-54350340 MS.gene42747:intron 50.0%
TTTCACTGCACAGGTTGCTC+AGG - chr8.4:54349912-54349931 MS.gene42747:CDS 50.0%
CATGCAGCCAAACCATCTGG+TGG + chr8.4:54350962-54350981 None:intergenic 55.0%
TCGAACGACTCGCAGTCAAG+TGG - chr8.4:54350346-54350365 MS.gene42747:intron 55.0%
! GAACTTGAGGGGTGTGCCTA+GGG - chr8.4:54350667-54350686 MS.gene42747:intron 55.0%
ACTGCGAGTCGTTCGAACCC+AGG + chr8.4:54350342-54350361 None:intergenic 60.0%
GGGTGTGCCTAGGGTTCCAT+GGG - chr8.4:54350676-54350695 MS.gene42747:intron 60.0%
! GGAACTTGAGGGGTGTGCCT+AGG - chr8.4:54350666-54350685 MS.gene42747:intron 60.0%
GGGGTGTGCCTAGGGTTCCA+TGG - chr8.4:54350675-54350694 MS.gene42747:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 54349775 54350988 54349775 ID=MS.gene42747
chr8.4 mRNA 54349775 54350988 54349775 ID=MS.gene42747.t1;Parent=MS.gene42747
chr8.4 exon 54350687 54350988 54350687 ID=MS.gene42747.t1.exon1;Parent=MS.gene42747.t1
chr8.4 CDS 54350687 54350988 54350687 ID=cds.MS.gene42747.t1;Parent=MS.gene42747.t1
chr8.4 exon 54349775 54350261 54349775 ID=MS.gene42747.t1.exon2;Parent=MS.gene42747.t1
chr8.4 CDS 54349775 54350261 54349775 ID=cds.MS.gene42747.t1;Parent=MS.gene42747.t1
Gene Sequence

>MS.gene42747

ATGGCTTTTCCTCTTTGCAATCTCAATCTTTCTCTATTACCTCAATCCAAATCAAGATTCAAACACCAAACACTGTCTTCTTCAAAACCCATTTCTCAATACTCTTTATTCTTCTCTTCTTCTCCTTCAGTCCAAAGTTTCACTGCACAGGTTGCTCAGGTGAATTCACCATTAGCTACTCAAGATAAACAGAATCAGCATCAAAAGGATGAGTTTTATCTCAATCTTGGTGTTGCTGTTAGAACCCTTCGTGAGGATATGCCTTTGATCTTTGTTAAAGACCTTAATTATGATATTTATAGGTAATTTTCTCATCTGGGTATGTAAAGTTTCTTGCTTTTCATATAAATATATGGTTTTTAATTTGAATTGTGTTATTTCTAGGTGAAAAAAAATTGGATTTTTAATGTTGGGTTTGGTAATTTTAAGGATGATGTAATTAATATTTATGGACCCTTTGGATGTGCATATATTGGTTTTTAATTCGAATTTAGTTAATTCTGAGTAATCTGTAAGGCCTAGCTCAACTGGTAAATGTCGATATAGTTAGGTCGGACACCTTAACCTGGGTTCGAACGACTCGCAGTCAAGTGGTGTATGAGAGTTTAAAACTCATTGCCACTTTCTATGTATAAAAAAAGTTAATTTTGAGTGGAAAAAAATTGGGTTTTTATTCTGTGCAAAAATATAGTTAGAATTTTAATGTTGGGTTTGATAATTTTTTCAGGGATGATGTAACATTTTTGGACCCTTTGAACACATTTACTGGTATTGAGAAGTACAAGTTGATCTTTTGGGCATTGAGGTTTCATGGTAGAATTTTGTTTCGTGAGATTGGTTTGGATGTATACAGAATATGGCAGCCTTCAGAGAATGTGATATTGATCAGGTGGAACTTGAGGGGTGTGCCTAGGGTTCCATGGGAAGCTAAAGGTGAATTTCAAGGTACTTCGCGGTATAAATTGGATCGGAACGGTAAGATTTATGAACACAAAGTTGATAATTTAGCATTCAACTTTCCACAGAAGATGATGAAACCGGTTTCGGTTTTGGATTTGGTTACTGCTTGCCCTGCTAGTCCAAATCCAACATTTTCATGGGGTCCTTTGGATTCTAGTTCATGTTCATGGATAGAATTATATCAAGCAGTTAAGCACACATTGGATGAAGAAAGACAGTTGTTACCACCAGATGGTTTGGCTGCATGTTCATAG

Protein sequence

>MS.gene42747.t1

MAFPLCNLNLSLLPQSKSRFKHQTLSSSKPISQYSLFFSSSPSVQSFTAQVAQVNSPLATQDKQNQHQKDEFYLNLGVAVRTLREDMPLIFVKDLNYDIYRDDVTFLDPLNTFTGIEKYKLIFWALRFHGRILFREIGLDVYRIWQPSENVILIRWNLRGVPRVPWEAKGEFQGTSRYKLDRNGKIYEHKVDNLAFNFPQKMMKPVSVLDLVTACPASPNPTFSWGPLDSSSCSWIELYQAVKHTLDEERQLLPPDGLAACS