Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049704.t1 | XP_003600461.1 | 98.1 | 310 | 4 | 1 | 1 | 308 | 1 | 310 | 2.50E-179 | 637.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049704.t1 | Q9FKT5 | 84.5 | 310 | 45 | 2 | 2 | 308 | 3 | 312 | 1.9e-156 | 553.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049704.t1 | G7IY98 | 98.1 | 310 | 4 | 1 | 1 | 308 | 1 | 310 | 1.8e-179 | 637.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene049704 | MS.gene012608 | PPI |
| MS.gene97981 | MS.gene049704 | PPI |
| MS.gene064894 | MS.gene049704 | PPI |
| MS.gene33535 | MS.gene049704 | PPI |
| MS.gene066026 | MS.gene049704 | PPI |
| MS.gene056487 | MS.gene049704 | PPI |
| MS.gene049704 | MS.gene97980 | PPI |
| MS.gene063165 | MS.gene049704 | PPI |
| MS.gene049704 | MS.gene71431 | PPI |
| MS.gene008794 | MS.gene049704 | PPI |
| MS.gene008793 | MS.gene049704 | PPI |
| MS.gene52980 | MS.gene049704 | PPI |
| MS.gene042253 | MS.gene049704 | PPI |
| MS.gene004825 | MS.gene049704 | PPI |
| MS.gene046235 | MS.gene049704 | PPI |
| MS.gene93058 | MS.gene049704 | PPI |
| MS.gene056488 | MS.gene049704 | PPI |
| MS.gene68222 | MS.gene049704 | PPI |
| MS.gene93059 | MS.gene049704 | PPI |
| MS.gene014281 | MS.gene049704 | PPI |
| MS.gene95244 | MS.gene049704 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049704.t1 | MTR_3g061450 | 98.065 | 310 | 4 | 1 | 1 | 308 | 1 | 310 | 0.0 | 634 |
| MS.gene049704.t1 | MTR_4g095058 | 28.014 | 282 | 186 | 7 | 11 | 280 | 280 | 556 | 1.08e-19 | 89.7 |
| MS.gene049704.t1 | MTR_4g095058 | 29.050 | 179 | 112 | 4 | 8 | 175 | 402 | 576 | 5.36e-15 | 75.9 |
| MS.gene049704.t1 | MTR_4g095058 | 23.934 | 305 | 202 | 8 | 17 | 294 | 228 | 529 | 3.42e-12 | 67.4 |
| MS.gene049704.t1 | MTR_4g095058 | 28.014 | 282 | 186 | 7 | 11 | 280 | 278 | 554 | 1.18e-19 | 89.7 |
| MS.gene049704.t1 | MTR_4g095058 | 29.050 | 179 | 112 | 4 | 8 | 175 | 400 | 574 | 5.61e-15 | 75.5 |
| MS.gene049704.t1 | MTR_4g095058 | 23.934 | 305 | 202 | 8 | 17 | 294 | 226 | 527 | 3.74e-12 | 67.0 |
| MS.gene049704.t1 | MTR_5g016370 | 28.488 | 172 | 112 | 3 | 13 | 175 | 424 | 593 | 1.73e-16 | 80.1 |
| MS.gene049704.t1 | MTR_5g016370 | 24.096 | 332 | 203 | 11 | 17 | 305 | 245 | 570 | 1.78e-15 | 77.0 |
| MS.gene049704.t1 | MTR_4g125710 | 24.436 | 266 | 177 | 6 | 13 | 256 | 54 | 317 | 2.39e-16 | 78.6 |
| MS.gene049704.t1 | MTR_5g016350 | 29.885 | 174 | 107 | 4 | 13 | 175 | 412 | 581 | 4.52e-16 | 79.0 |
| MS.gene049704.t1 | MTR_5g016350 | 24.545 | 330 | 206 | 8 | 17 | 305 | 231 | 558 | 6.43e-16 | 78.6 |
| MS.gene049704.t1 | MTR_5g016350 | 25.000 | 280 | 153 | 10 | 20 | 247 | 295 | 569 | 7.96e-12 | 66.2 |
| MS.gene049704.t1 | MTR_5g016350 | 29.885 | 174 | 107 | 4 | 13 | 175 | 348 | 517 | 7.24e-16 | 78.2 |
| MS.gene049704.t1 | MTR_5g016350 | 24.545 | 330 | 206 | 8 | 17 | 305 | 167 | 494 | 7.30e-16 | 78.2 |
| MS.gene049704.t1 | MTR_5g016350 | 25.000 | 280 | 153 | 10 | 20 | 247 | 231 | 505 | 8.56e-12 | 65.9 |
| MS.gene049704.t1 | MTR_4g119620 | 25.620 | 242 | 169 | 6 | 13 | 247 | 72 | 309 | 6.50e-15 | 74.3 |
| MS.gene049704.t1 | MTR_3g106080 | 26.400 | 250 | 123 | 5 | 15 | 247 | 457 | 662 | 1.36e-14 | 74.7 |
| MS.gene049704.t1 | MTR_1g097140 | 25.969 | 258 | 181 | 7 | 12 | 265 | 116 | 367 | 3.04e-14 | 73.2 |
| MS.gene049704.t1 | MTR_1g072140 | 22.867 | 293 | 214 | 8 | 11 | 295 | 19 | 307 | 8.42e-14 | 71.2 |
| MS.gene049704.t1 | MTR_5g090420 | 24.561 | 285 | 202 | 8 | 18 | 293 | 263 | 543 | 1.24e-12 | 68.6 |
| MS.gene049704.t1 | MTR_6g004040 | 27.386 | 241 | 158 | 8 | 13 | 242 | 5 | 239 | 2.08e-12 | 68.2 |
| MS.gene049704.t1 | MTR_3g449990 | 24.739 | 287 | 202 | 9 | 17 | 293 | 268 | 550 | 3.66e-12 | 67.0 |
| MS.gene049704.t1 | MTR_7g086360 | 20.608 | 296 | 220 | 7 | 16 | 299 | 52 | 344 | 4.42e-12 | 66.2 |
| MS.gene049704.t1 | MTR_5g022690 | 25.481 | 208 | 145 | 6 | 13 | 215 | 273 | 475 | 4.38e-11 | 63.9 |
| MS.gene049704.t1 | MTR_2g016160 | 31.061 | 132 | 80 | 4 | 13 | 138 | 101 | 227 | 5.24e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049704.t1 | AT5G56130 | 84.615 | 312 | 44 | 3 | 1 | 308 | 1 | 312 | 0.0 | 551 |
| MS.gene049704.t1 | AT5G67320 | 28.521 | 284 | 182 | 10 | 11 | 280 | 315 | 591 | 4.67e-20 | 90.9 |
| MS.gene049704.t1 | AT5G67320 | 30.058 | 173 | 110 | 3 | 12 | 175 | 441 | 611 | 6.86e-16 | 78.6 |
| MS.gene049704.t1 | AT2G43770 | 23.549 | 293 | 209 | 8 | 16 | 296 | 50 | 339 | 1.92e-15 | 76.3 |
| MS.gene049704.t1 | AT5G25150 | 25.794 | 252 | 122 | 6 | 15 | 247 | 456 | 661 | 8.33e-14 | 72.4 |
| MS.gene049704.t1 | AT5G08560 | 25.087 | 287 | 195 | 9 | 31 | 308 | 286 | 561 | 2.94e-13 | 70.5 |
| MS.gene049704.t1 | AT5G08560 | 25.087 | 287 | 195 | 9 | 31 | 308 | 286 | 561 | 2.94e-13 | 70.5 |
| MS.gene049704.t1 | AT5G08560 | 25.087 | 287 | 195 | 9 | 31 | 308 | 286 | 561 | 2.94e-13 | 70.5 |
| MS.gene049704.t1 | AT4G02730 | 27.143 | 210 | 141 | 8 | 14 | 215 | 80 | 285 | 3.63e-13 | 69.3 |
| MS.gene049704.t1 | AT5G25150 | 27.941 | 204 | 98 | 5 | 15 | 215 | 456 | 613 | 5.21e-13 | 69.7 |
| MS.gene049704.t1 | AT3G49660 | 24.172 | 302 | 202 | 11 | 10 | 293 | 15 | 307 | 2.13e-11 | 63.9 |
| MS.gene049704.t1 | AT3G05090 | 31.933 | 119 | 77 | 3 | 17 | 134 | 211 | 326 | 5.18e-11 | 63.9 |
| MS.gene049704.t1 | AT3G05090 | 31.933 | 119 | 77 | 3 | 17 | 134 | 211 | 326 | 5.18e-11 | 63.9 |
| MS.gene049704.t1 | AT3G05090 | 31.933 | 119 | 77 | 3 | 17 | 134 | 211 | 326 | 5.18e-11 | 63.9 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTCAGCTTGTGGGTTATTT+TGG | 0.143353 | 3.3:+58319033 | MS.gene049704:CDS |
| TTGATCAAACTGCTCGAATT+TGG | 0.221602 | 3.3:+58312654 | MS.gene049704:CDS |
| TATGCAAGTACAATGTATTT+AGG | 0.241095 | 3.3:-58319847 | None:intergenic |
| ACAATTAGTTTCAATCATAC+TGG | 0.283022 | 3.3:+58319212 | MS.gene049704:CDS |
| TGCAATAAGATCGGGATGTT+TGG | 0.289165 | 3.3:-58313738 | None:intergenic |
| CTCATGCAGCTGTAGGTAAT+AGG | 0.294348 | 3.3:+58314052 | MS.gene049704:CDS |
| GCAATAAGATCGGGATGTTT+GGG | 0.297004 | 3.3:-58313737 | None:intergenic |
| GTGACAAGACTGTTCGTCTA+TGG | 0.297252 | 3.3:+58313771 | MS.gene049704:CDS |
| CGATCTTATTGCAACTGCTT+CGG | 0.300112 | 3.3:+58313748 | MS.gene049704:CDS |
| GATCTTATTGCAACTGCTTC+GGG | 0.329060 | 3.3:+58313749 | MS.gene049704:CDS |
| TTCTTTCTAACAACAGGAAA+TGG | 0.391405 | 3.3:+58318619 | MS.gene049704:CDS |
| CTGATTCCCTTGTCAGCTTG+TGG | 0.395348 | 3.3:+58319023 | MS.gene049704:CDS |
| TGATTCCCTTGTCAGCTTGT+GGG | 0.409663 | 3.3:+58319024 | MS.gene049704:CDS |
| CAGTACATCATATTCCTTGT+AGG | 0.413376 | 3.3:+58319797 | MS.gene049704:CDS |
| CTTCACAGCAGAGAGTATTC+TGG | 0.419755 | 3.3:+58312410 | MS.gene049704:CDS |
| TGCATTGCAATTGACCCAAC+AGG | 0.430132 | 3.3:+58318826 | MS.gene049704:CDS |
| AAAAGGTGCATTCAGTGGCT+TGG | 0.441542 | 3.3:+58312597 | MS.gene049704:intron |
| CCTAGGTAAATGAGATTGCT+TGG | 0.445389 | 3.3:+58318578 | MS.gene049704:intron |
| GGTCAAAGATATTGAATTGA+AGG | 0.446383 | 3.3:+58313679 | MS.gene049704:intron |
| AGTTAACAATACTTGATGTT+CGG | 0.447811 | 3.3:+58314301 | MS.gene049704:CDS |
| ATTCCACTCAACACTATTCA+TGG | 0.456424 | 3.3:-58319824 | None:intergenic |
| GAGATGTTCTTTCTAACAAC+AGG | 0.457171 | 3.3:+58318613 | MS.gene049704:CDS |
| ATGAGTACCATCAGGTTTGT+AGG | 0.457397 | 3.3:-58314034 | None:intergenic |
| ATTGGCACAAAACTTGCTTC+TGG | 0.457664 | 3.3:+58312626 | MS.gene049704:CDS |
| CAATTAGTTTCAATCATACT+GGG | 0.469646 | 3.3:+58319213 | MS.gene049704:CDS |
| TATGATTGAAACTAATTGTC+CGG | 0.470565 | 3.3:-58319208 | None:intergenic |
| TGTTTCTCAGATGGCCTGTC+CGG | 0.480041 | 3.3:+58319189 | MS.gene049704:intron |
| TCATGCAGCTGTAGGTAATA+GGG | 0.497568 | 3.3:+58314053 | MS.gene049704:CDS |
| AGGTGGAAAATGCTCACAAC+AGG | 0.499837 | 3.3:+58313981 | MS.gene049704:intron |
| CTATTCATGGCAGCCCTACA+AGG | 0.501447 | 3.3:-58319811 | None:intergenic |
| CGAAGCAGTTGCAATAAGAT+CGG | 0.502914 | 3.3:-58313747 | None:intergenic |
| CACAACAGGCAGAACTTAGT+GGG | 0.503581 | 3.3:+58313995 | MS.gene049704:CDS |
| GTATCAAGAGGTCGAAGAGA+TGG | 0.505442 | 3.3:-58318775 | None:intergenic |
| TAGTGTGGATCAGCTATGTT+GGG | 0.512894 | 3.3:+58313712 | MS.gene049704:CDS |
| TGACAAGACTGTTCGTCTAT+GGG | 0.513043 | 3.3:+58313772 | MS.gene049704:CDS |
| AACTTACTCGAGCTTTGTAA+AGG | 0.513452 | 3.3:-58319074 | None:intergenic |
| AAGAAGCATACCTTCCTGTT+GGG | 0.521567 | 3.3:-58318840 | None:intergenic |
| TCAGTGGCTTGGAATTGCAT+TGG | 0.521845 | 3.3:+58312608 | MS.gene049704:CDS |
| TCACAACAGGCAGAACTTAG+TGG | 0.530916 | 3.3:+58313994 | MS.gene049704:CDS |
| AAACGAGAATTGTTCCTCCA+TGG | 0.533256 | 3.3:-58312381 | None:intergenic |
| GAAGCAGTTGCAATAAGATC+GGG | 0.535149 | 3.3:-58313746 | None:intergenic |
| TTGAACTTACGCCTATGCAT+TGG | 0.535702 | 3.3:-58314333 | None:intergenic |
| TACATTGTACTTGCATATGC+CGG | 0.539937 | 3.3:+58319853 | MS.gene049704:CDS |
| AGTACATCATATTCCTTGTA+GGG | 0.547208 | 3.3:+58319798 | MS.gene049704:CDS |
| CAAGAAGCATACCTTCCTGT+TGG | 0.550714 | 3.3:-58318841 | None:intergenic |
| TACTCTCATGGCTCATACAG+CGG | 0.555050 | 3.3:+58318795 | MS.gene049704:CDS |
| ACTCTCATGGCTCATACAGC+GGG | 0.555763 | 3.3:+58318796 | MS.gene049704:CDS |
| AAATAACCCACAAGCTGACA+AGG | 0.557638 | 3.3:-58319030 | None:intergenic |
| ATAGTGTGGATCAGCTATGT+TGG | 0.561550 | 3.3:+58313711 | MS.gene049704:CDS |
| TCGACCTCTTGATACTCTCA+TGG | 0.565472 | 3.3:+58318783 | MS.gene049704:CDS |
| TGACAGTCCAATGTTCATAC+CGG | 0.566154 | 3.3:+58319769 | MS.gene049704:intron |
| GTCAAAGATATTGAATTGAA+GGG | 0.579788 | 3.3:+58313680 | MS.gene049704:CDS |
| TAGGCGTAAGTTCAACTATG+AGG | 0.584012 | 3.3:+58314341 | MS.gene049704:CDS |
| GGAAATTCAAACCAATGCAT+AGG | 0.586683 | 3.3:+58314322 | MS.gene049704:CDS |
| ACAGCTGCATGAGTACCATC+AGG | 0.587950 | 3.3:-58314042 | None:intergenic |
| TTGAAACTAATTGTCCGGAC+AGG | 0.589710 | 3.3:-58319203 | None:intergenic |
| AAGGTTACTACATGGGCATG+TGG | 0.595342 | 3.3:-58312686 | None:intergenic |
| GTTTCAAAAGGTGCATTCAG+TGG | 0.599434 | 3.3:+58312592 | MS.gene049704:intron |
| GAGATTGCTTGGAACATGAC+TGG | 0.603670 | 3.3:+58318589 | MS.gene049704:CDS |
| GAAGGGTCACACCGATAGTG+TGG | 0.604201 | 3.3:+58313697 | MS.gene049704:CDS |
| AACATCACCTACAAACCTGA+TGG | 0.607731 | 3.3:+58314027 | MS.gene049704:CDS |
| GATGGTACTCATGCAGCTGT+AGG | 0.608815 | 3.3:+58314045 | MS.gene049704:CDS |
| AGGTTACTACATGGGCATGT+GGG | 0.614543 | 3.3:-58312685 | None:intergenic |
| CAATAAGATCGGGATGTTTG+GGG | 0.614895 | 3.3:-58313736 | None:intergenic |
| ACATAGCTGATCCACACTAT+CGG | 0.628331 | 3.3:-58313708 | None:intergenic |
| AACAAGTATCAGGCTGACGA+AGG | 0.633197 | 3.3:+58319886 | MS.gene049704:CDS |
| CTGCCATGAATAGTGTTGAG+TGG | 0.656405 | 3.3:+58319821 | MS.gene049704:CDS |
| TTGCAATTGACCCAACAGGA+AGG | 0.660962 | 3.3:+58318830 | MS.gene049704:CDS |
| AATAACCCACAAGCTGACAA+GGG | 0.681570 | 3.3:-58319029 | None:intergenic |
| AATTAGTTTCAATCATACTG+GGG | 0.695286 | 3.3:+58319214 | MS.gene049704:CDS |
| TGAGCCATGAGAGTATCAAG+AGG | 0.696186 | 3.3:-58318787 | None:intergenic |
| ACAACAGGCAGAACTTAGTG+GGG | 0.714393 | 3.3:+58313996 | MS.gene049704:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58312383 | 58320020 | 58312383 | ID=MS.gene049704 |
| chr3.3 | mRNA | 58312383 | 58320020 | 58312383 | ID=MS.gene049704.t1;Parent=MS.gene049704 |
| chr3.3 | exon | 58312383 | 58312445 | 58312383 | ID=MS.gene049704.t1.exon1;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58312383 | 58312445 | 58312383 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58312602 | 58312697 | 58312602 | ID=MS.gene049704.t1.exon2;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58312602 | 58312697 | 58312602 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58313680 | 58313803 | 58313680 | ID=MS.gene049704.t1.exon3;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58313680 | 58313803 | 58313680 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58313983 | 58314074 | 58313983 | ID=MS.gene049704.t1.exon4;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58313983 | 58314074 | 58313983 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58314294 | 58314362 | 58314294 | ID=MS.gene049704.t1.exon5;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58314294 | 58314362 | 58314294 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58318583 | 58318640 | 58318583 | ID=MS.gene049704.t1.exon6;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58318583 | 58318640 | 58318583 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58318752 | 58318851 | 58318752 | ID=MS.gene049704.t1.exon7;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58318752 | 58318851 | 58318752 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58319003 | 58319089 | 58319003 | ID=MS.gene049704.t1.exon8;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58319003 | 58319089 | 58319003 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58319199 | 58319277 | 58319199 | ID=MS.gene049704.t1.exon9;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58319199 | 58319277 | 58319199 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58319775 | 58319907 | 58319775 | ID=MS.gene049704.t1.exon10;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58319775 | 58319907 | 58319775 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| chr3.3 | exon | 58319995 | 58320020 | 58319995 | ID=MS.gene049704.t1.exon11;Parent=MS.gene049704.t1 |
| chr3.3 | CDS | 58319995 | 58320020 | 58319995 | ID=cds.MS.gene049704.t1;Parent=MS.gene049704.t1 |
| Gene Sequence |
| Protein sequence |
