AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99003

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99003.t1 MTR_1g054285 94.408 304 7 4 1 302 1 296 0.0 570
MS.gene99003.t1 MTR_7g093430 68.364 275 75 7 1 265 1 273 8.30e-118 341
MS.gene99003.t1 MTR_2g061030 51.479 338 92 10 1 278 1 326 6.56e-80 246
MS.gene99003.t1 MTR_4g100550 59.535 215 66 5 81 291 57 254 3.58e-74 229
MS.gene99003.t1 MTR_5g013010 62.564 195 50 5 81 266 71 251 2.88e-71 222
MS.gene99003.t1 MTR_4g097600 79.070 129 25 1 138 264 111 239 2.12e-68 214
MS.gene99003.t1 MTR_8g006705 62.162 185 39 4 98 263 86 258 7.54e-64 203
MS.gene99003.t1 MTR_5g014890 75.000 124 30 1 143 265 113 236 4.31e-62 198
MS.gene99003.t1 MTR_1g017090 55.249 181 64 5 94 265 73 245 1.31e-54 179
MS.gene99003.t1 MTR_4g084750 65.323 124 36 2 147 263 59 182 6.18e-47 156
MS.gene99003.t1 MTR_3g103590 63.235 136 49 1 143 278 136 270 1.29e-45 156
MS.gene99003.t1 MTR_2g038625 56.296 135 49 3 135 264 66 195 4.98e-39 137
MS.gene99003.t1 MTR_2g038580 56.296 135 49 3 135 264 66 195 4.98e-39 137
MS.gene99003.t1 MTR_4g126900 57.018 114 45 1 154 263 90 203 3.11e-34 124
MS.gene99003.t1 MTR_5g013010 53.435 131 39 4 81 203 71 187 5.97e-32 118
MS.gene99003.t1 MTR_7g010020 37.647 170 97 3 111 276 40 204 1.05e-21 92.4
MS.gene99003.t1 MTR_4g107650 46.667 120 60 2 120 239 67 182 1.79e-19 87.4
MS.gene99003.t1 MTR_3g086790 34.973 183 108 4 102 274 10 191 2.00e-18 84.0
MS.gene99003.t1 MTR_1g061660 44.086 93 52 0 148 240 55 147 2.20e-18 82.8
MS.gene99003.t1 MTR_5g039000 46.875 96 51 0 144 239 69 164 3.21e-18 83.2
MS.gene99003.t1 MTR_8g468210 42.609 115 62 1 135 245 60 174 5.31e-18 82.4
MS.gene99003.t1 MTR_6g007647 41.026 117 69 0 149 265 30 146 6.88e-18 81.6
MS.gene99003.t1 MTR_7g103340 42.105 95 55 0 145 239 56 150 1.18e-17 80.5
MS.gene99003.t1 MTR_6g007647 41.026 117 69 0 149 265 64 180 1.32e-17 81.6
MS.gene99003.t1 MTR_3g080100 46.226 106 48 2 143 239 9 114 4.47e-17 78.6
MS.gene99003.t1 MTR_8g089895 43.200 125 61 2 122 239 27 148 5.35e-17 80.1
MS.gene99003.t1 MTR_3g080100 46.226 106 48 2 143 239 48 153 6.54e-17 79.0
MS.gene99003.t1 MTR_6g011610 39.865 148 75 4 104 238 43 189 6.63e-17 79.3
MS.gene99003.t1 MTR_5g038280 46.809 94 43 1 152 238 87 180 2.38e-16 78.6
MS.gene99003.t1 MTR_6g011610 44.348 115 51 3 137 238 56 170 2.73e-16 77.4
MS.gene99003.t1 MTR_8g469430 44.681 94 45 1 152 238 89 182 3.50e-15 74.7
MS.gene99003.t1 MTR_5g019650 40.741 108 60 1 129 236 34 137 2.85e-11 62.4
MS.gene99003.t1 MTR_8g026960 44.595 74 41 0 164 237 46 119 5.18e-11 62.0
MS.gene99003.t1 MTR_5g019680 40.000 110 62 1 129 238 34 139 6.05e-11 61.2
MS.gene99003.t1 MTR_3g092150 40.625 96 50 1 155 243 41 136 6.87e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99003.t1 AT5G06710 51.235 324 82 9 1 267 1 305 1.03e-86 263
MS.gene99003.t1 AT5G47370 52.075 265 104 11 48 297 22 278 2.11e-70 219
MS.gene99003.t1 AT3G60390 59.406 202 60 6 81 263 76 274 1.36e-68 216
MS.gene99003.t1 AT4G37790 79.508 122 25 0 143 264 117 238 7.50e-66 208
MS.gene99003.t1 AT4G16780 57.870 216 70 7 81 290 68 268 6.16e-65 206
MS.gene99003.t1 AT4G17460 49.624 266 84 10 48 283 21 266 1.88e-64 204
MS.gene99003.t1 AT2G44910 57.426 202 63 5 81 266 84 278 7.95e-64 204
MS.gene99003.t1 AT2G22800 77.049 122 28 0 143 264 104 225 1.25e-63 202
MS.gene99003.t1 AT2G01430 50.000 172 68 4 102 264 87 249 1.38e-44 153
MS.gene99003.t1 AT1G70920 59.124 137 42 4 142 274 27 153 4.41e-41 140
MS.gene99003.t1 AT1G70920 59.124 137 42 4 142 274 57 183 4.71e-41 142
MS.gene99003.t1 AT5G06710 41.923 260 75 9 1 203 1 241 3.56e-40 141
MS.gene99003.t1 AT2G01430 45.045 111 45 2 102 203 87 190 1.81e-20 87.8
MS.gene99003.t1 AT5G06710 36.052 233 73 9 1 176 1 214 3.26e-20 87.8
MS.gene99003.t1 AT5G06710 36.052 233 73 9 1 176 1 214 3.26e-20 87.8
MS.gene99003.t1 AT1G69780 44.340 106 59 0 134 239 67 172 3.57e-18 83.2
MS.gene99003.t1 AT1G26960 44.860 107 55 1 144 246 63 169 9.83e-18 81.3
MS.gene99003.t1 AT5G03790 39.130 115 69 1 145 258 70 184 2.40e-17 80.1
MS.gene99003.t1 AT2G36610 41.176 102 52 1 147 240 66 167 2.02e-16 76.3
MS.gene99003.t1 AT5G15150 43.590 117 61 3 130 241 92 208 9.13e-16 76.6
MS.gene99003.t1 AT3G01220 44.330 97 50 1 152 244 87 183 3.36e-15 74.7
MS.gene99003.t1 AT2G22430 45.455 99 50 1 148 242 58 156 4.24e-15 74.7
MS.gene99003.t1 AT3G01470 44.565 92 51 0 152 243 68 159 4.56e-15 74.3
MS.gene99003.t1 AT1G27045 57.576 66 28 0 152 217 17 82 1.71e-14 70.9
MS.gene99003.t1 AT1G27045 57.576 66 28 0 152 217 25 90 1.81e-14 70.9
MS.gene99003.t1 AT1G27045 57.576 66 28 0 152 217 25 90 1.81e-14 70.9
MS.gene99003.t1 AT1G27045 57.576 66 28 0 152 217 68 133 2.77e-14 71.2
MS.gene99003.t1 AT4G40060 43.878 98 51 1 148 241 55 152 2.86e-14 72.0
MS.gene99003.t1 AT5G65310 41.837 98 50 1 149 239 69 166 3.89e-14 72.0
MS.gene99003.t1 AT5G65310 41.837 98 50 1 149 239 51 148 4.05e-14 71.6
MS.gene99003.t1 AT1G27045 57.576 66 28 0 152 217 68 133 4.35e-14 71.2
MS.gene99003.t1 AT5G03790 35.772 123 71 2 145 259 70 192 6.02e-14 69.7
MS.gene99003.t1 AT2G18550 44.554 101 54 2 138 238 49 147 1.38e-13 68.9
MS.gene99003.t1 AT2G46680 40.659 91 54 0 147 237 27 117 6.58e-13 67.8
MS.gene99003.t1 AT2G46680 40.659 91 54 0 147 237 27 117 7.42e-13 67.8
MS.gene99003.t1 AT4G36740 41.237 97 56 1 142 238 47 142 4.15e-12 64.7
MS.gene99003.t1 AT4G36740 41.237 97 56 1 142 238 46 141 4.25e-12 64.7
MS.gene99003.t1 AT3G61890 40.964 83 49 0 155 237 33 115 8.41e-11 61.2

Find 74 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCTTGAGCCTGATGGGCT+TGG 0.185004 1.2:+40858724 None:intergenic
GGCTTGAGCCTGATGGGCTT+GGG 0.249675 1.2:+40858725 None:intergenic
AGAAAAGAGATAGGTAATTT+TGG 0.264059 1.2:+40860566 None:intergenic
AGGAATAACAATGCTGAATA+TGG 0.265099 1.2:-40859861 MS.gene99003:CDS
GGAAGTGATGATGATGAAAA+TGG 0.308043 1.2:-40859798 MS.gene99003:CDS
CTTCTCACCTCCCCATTCCT+TGG 0.329294 1.2:-40860400 MS.gene99003:CDS
GAAACTTGGTAGTGGATCTT+TGG 0.333786 1.2:+40860532 None:intergenic
GGCTTGGGCTTGAGCCTGAT+GGG 0.342578 1.2:+40858719 None:intergenic
GGGCTTGGGCTTGAGCCTGA+TGG 0.356666 1.2:+40858718 None:intergenic
TTGACTTGTTGGTGTTTGTT+TGG 0.360920 1.2:+40858775 None:intergenic
ATGGAACTTGCTTTGAGCTT+AGG 0.368374 1.2:-40860596 MS.gene99003:CDS
GGAAGAAGGTCTAGTTGAAT+AGG 0.392211 1.2:+40860455 None:intergenic
GTCCTCGTCAAGTTGAAGTT+TGG 0.396562 1.2:-40859558 MS.gene99003:CDS
GCTAGACGTGAACCGTTTCC+CGG 0.397577 1.2:-40859970 MS.gene99003:CDS
TGCTCAAGCCCAAGCCCATC+AGG 0.400227 1.2:-40858733 MS.gene99003:CDS
ACGTCTTCGGTCATCGCCGC+CGG 0.416077 1.2:+40859951 None:intergenic
CGGTCATCGCCGCCGGGAAA+CGG 0.418907 1.2:+40859958 None:intergenic
ATGAAGGAATCTGATGGGCT+TGG 0.420734 1.2:+40858703 None:intergenic
GGTTGGGTGAGCCAAGGAAT+GGG 0.421883 1.2:+40860389 None:intergenic
TTCACGTCTAGCTCCCTTAC+CGG 0.422442 1.2:+40859981 None:intergenic
TGGCAGTATTCTTGACTTGT+TGG 0.444669 1.2:+40858764 None:intergenic
TAGATAGAAAATTACATGGT+TGG 0.446528 1.2:+40860372 None:intergenic
TGAAGGAATCTGATGGGCTT+GGG 0.452845 1.2:+40858704 None:intergenic
TTGCAATGGATTTCAGTATG+AGG 0.457926 1.2:-40859881 MS.gene99003:CDS
GTGTTTGTTTGGTTGTTGCA+AGG 0.458788 1.2:+40858786 None:intergenic
AGATAGAAAATTACATGGTT+GGG 0.461778 1.2:+40860373 None:intergenic
TTCTGTGTCACCATTTGCAA+TGG 0.477662 1.2:-40859895 MS.gene99003:CDS
CACATAGTGAGAGTGGTAGC+TGG 0.479169 1.2:+40858837 None:intergenic
GGGTGAGGAGATCTAAGAAC+AGG 0.479275 1.2:+40860425 None:intergenic
GGAATGGGGAGGTGAGAAGA+AGG 0.480630 1.2:+40860404 None:intergenic
GGTGCCGTCGTCGACGTCTT+CGG 0.486891 1.2:+40859938 None:intergenic
CTTGGTAGTGGATCTTTGGT+GGG 0.487093 1.2:+40860536 None:intergenic
TGTTTGTTTGGTTGTTGCAA+GGG 0.493080 1.2:+40858787 None:intergenic
ATACTGAAATCCATTGCAAA+TGG 0.496930 1.2:+40859885 None:intergenic
GATCTAAGAACAGGAGAGAA+AGG 0.503681 1.2:+40860434 None:intergenic
TGGTTGGGTGAGCCAAGGAA+TGG 0.507151 1.2:+40860388 None:intergenic
AGAAACAAGAGAGAAAATGA+AGG 0.510237 1.2:-40859834 MS.gene99003:CDS
ACTTGGTAGTGGATCTTTGG+TGG 0.512252 1.2:+40860535 None:intergenic
TTTGTTATCATCAGGTAGAC+CGG 0.513453 1.2:-40860000 MS.gene99003:intron
AATGAAGGTGAAGCTGAAAG+AGG 0.521199 1.2:-40859819 MS.gene99003:CDS
GAATGGGGAGGTGAGAAGAA+GGG 0.522886 1.2:+40860405 None:intergenic
TATCATCAGGTAGACCGGTA+AGG 0.523658 1.2:-40859995 MS.gene99003:intron
TGGGCTTGAGCATTAGAGAA+TGG 0.523806 1.2:+40858744 None:intergenic
GAAGTGATGATGATGAAAAT+GGG 0.525282 1.2:-40859797 MS.gene99003:CDS
GGGAGGTGAGAAGAAGGGTG+AGG 0.527359 1.2:+40860410 None:intergenic
AAATGGTGACACAGAACTGC+TGG 0.531470 1.2:+40859902 None:intergenic
TGTTGGGTTGAGAAGAAACT+TGG 0.533644 1.2:+40860518 None:intergenic
GTTGCTACACGTTCACAAGA+AGG 0.535467 1.2:+40858813 None:intergenic
CGTCTTCGGTCATCGCCGCC+GGG 0.545627 1.2:+40859952 None:intergenic
ATGATGAAAATGGGTCAACT+AGG 0.551419 1.2:-40859788 MS.gene99003:CDS
GGTTTGTCAAGAAAAGAGAT+AGG 0.551628 1.2:+40860557 None:intergenic
ATCATCAGGTAGACCGGTAA+GGG 0.553491 1.2:-40859994 MS.gene99003:intron
GAACAGGAGAGAAAGGAAGA+AGG 0.553836 1.2:+40860441 None:intergenic
AAGGTCATGAAGGAATCTGA+TGG 0.569756 1.2:+40858697 None:intergenic
GTTGAGAAGAAACTTGGTAG+TGG 0.575951 1.2:+40860524 None:intergenic
GAAATTTACAGGATTAAGAG+TGG 0.579010 1.2:+40859704 None:intergenic
AAAGCTAAAGCAAACAGAAG+TGG 0.588208 1.2:-40858976 MS.gene99003:CDS
GCTGGAAGTTGCATGTAGAA+TGG 0.591856 1.2:+40858855 None:intergenic
ATAAGATTATAAGGTCATGA+AGG 0.594650 1.2:+40858687 None:intergenic
AGAAGGTCTAGTTGAATAGG+TGG 0.598462 1.2:+40860458 None:intergenic
AGAAGGACACATAGTGAGAG+TGG 0.600200 1.2:+40858830 None:intergenic
GGTTTCAAAATAGAAGAGCA+AGG 0.607323 1.2:-40859537 MS.gene99003:intron
AATGGTGACACAGAACTGCT+GGG 0.607777 1.2:+40859903 None:intergenic
AGTTTCTTCTCAACCCAACA+AGG 0.609680 1.2:-40860515 MS.gene99003:CDS
TTACATGGTTGGGTGAGCCA+AGG 0.611815 1.2:+40860383 None:intergenic
AGGTCATGAAGGAATCTGAT+GGG 0.612514 1.2:+40858698 None:intergenic
AATAACAATGCTGAATATGG+AGG 0.617867 1.2:-40859858 MS.gene99003:CDS
AACCAAACTTCAACTTGACG+AGG 0.618703 1.2:+40859556 None:intergenic
GTTGGGTGAGCCAAGGAATG+GGG 0.619312 1.2:+40860390 None:intergenic
ATGGTGACACAGAACTGCTG+GGG 0.640069 1.2:+40859904 None:intergenic
ATGACCGAAGACGTCGACGA+CGG 0.659370 1.2:-40859942 MS.gene99003:CDS
GGGTGAGCCAAGGAATGGGG+AGG 0.690782 1.2:+40860393 None:intergenic
AGACGTGAACCGTTTCCCGG+CGG 0.739526 1.2:-40859967 MS.gene99003:CDS
TGGTGACACAGAACTGCTGG+GGG 0.743324 1.2:+40859905 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAAACAAAAAAATAC+TGG + chr1.2:40860129-40860148 None:intergenic 10.0%
!! AAATTAAATAAGAAATTTAC+AGG + chr1.2:40859607-40859626 None:intergenic 10.0%
!! AATTAAAACAAAAATATTGA+AGG + chr1.2:40859796-40859815 None:intergenic 10.0%
!!! TTTGTTTTAATTTTTTATCT+AGG - chr1.2:40859804-40859823 MS.gene99003:CDS 10.0%
!!! TTTTTATTAAAGTTTTGTTT+TGG - chr1.2:40860278-40860297 MS.gene99003:intron 10.0%
!! AATATATAAAAACCATTTAG+AGG + chr1.2:40858969-40858988 None:intergenic 15.0%
!! TATTCATATTCTAACTTATT+AGG + chr1.2:40860008-40860027 None:intergenic 15.0%
!!! TGAAAAGTCAATTTTTTTAA+TGG - chr1.2:40859161-40859180 MS.gene99003:intron 15.0%
!!! TTGTTTCTTCTTTTTAATTA+AGG - chr1.2:40859639-40859658 MS.gene99003:intron 15.0%
!! ATAGTAGATAGAAAATTACA+TGG + chr1.2:40858932-40858951 None:intergenic 20.0%
!! CAAAATGACATATATTTATG+AGG + chr1.2:40859906-40859925 None:intergenic 20.0%
!! GACTTAAATAGATAAGTAAA+TGG + chr1.2:40860245-40860264 None:intergenic 20.0%
!! TGTTAGATTTCTTTATCTTA+TGG + chr1.2:40860192-40860211 None:intergenic 20.0%
!!! AGAATTCTTTTGATATTTGT+TGG - chr1.2:40860217-40860236 MS.gene99003:intron 20.0%
!!! TCAATTTTTTTAATGGATCT+AGG - chr1.2:40859168-40859187 MS.gene99003:intron 20.0%
!!! TCTTATGGTTTTGTTTTTTT+TGG + chr1.2:40860177-40860196 None:intergenic 20.0%
!!! TTTTTGGTTACATGAAAAAA+AGG + chr1.2:40860161-40860180 None:intergenic 20.0%
!!! TTTTTGTTTTTGTTATCATC+AGG - chr1.2:40859289-40859308 MS.gene99003:intron 20.0%
! AGATAGAAAATTACATGGTT+GGG + chr1.2:40858927-40858946 None:intergenic 25.0%
! TAGATAGAAAATTACATGGT+TGG + chr1.2:40858928-40858947 None:intergenic 25.0%
!! AGAAAAGAGATAGGTAATTT+TGG + chr1.2:40858734-40858753 None:intergenic 25.0%
!! TTAAAGTGCTTGAAAATTGT+GGG - chr1.2:40859064-40859083 MS.gene99003:intron 25.0%
!! TTTAAAGTGCTTGAAAATTG+TGG - chr1.2:40859063-40859082 MS.gene99003:intron 25.0%
!!! TTTGGAATGTATGATTTTGT+AGG - chr1.2:40860296-40860315 MS.gene99003:intron 25.0%
AAAACCTTGTACCAAATCTT+GGG + chr1.2:40859102-40859121 None:intergenic 30.0%
AAAGCTTCAGAAGAAAAAAG+AGG - chr1.2:40858806-40858825 MS.gene99003:CDS 30.0%
AACCATTTAGAGGAAAGAAA+AGG + chr1.2:40858959-40858978 None:intergenic 30.0%
AATAACAATGCTGAATATGG+AGG - chr1.2:40859439-40859458 MS.gene99003:intron 30.0%
AGAAACAAGAGAGAAAATGA+AGG - chr1.2:40859463-40859482 MS.gene99003:intron 30.0%
AGGAATAACAATGCTGAATA+TGG - chr1.2:40859436-40859455 MS.gene99003:intron 30.0%
ATACTGAAATCCATTGCAAA+TGG + chr1.2:40859415-40859434 None:intergenic 30.0%
GAAATTTACAGGATTAAGAG+TGG + chr1.2:40859596-40859615 None:intergenic 30.0%
! ATCCTTTTCTTTCCTCTAAA+TGG - chr1.2:40858954-40858973 MS.gene99003:CDS 30.0%
! GAAGTGATGATGATGAAAAT+GGG - chr1.2:40859500-40859519 MS.gene99003:intron 30.0%
!!! ATATGTCATTTTGTCTCTTC+AGG - chr1.2:40859913-40859932 MS.gene99003:CDS 30.0%
AAAGCTAAAGCAAACAGAAG+TGG - chr1.2:40860321-40860340 MS.gene99003:intron 35.0%
ATGATGAAAATGGGTCAACT+AGG - chr1.2:40859509-40859528 MS.gene99003:intron 35.0%
CAAAACCTTGTACCAAATCT+TGG + chr1.2:40859103-40859122 None:intergenic 35.0%
GGTTTCAAAATAGAAGAGCA+AGG - chr1.2:40859760-40859779 MS.gene99003:CDS 35.0%
GGTTTGTCAAGAAAAGAGAT+AGG + chr1.2:40858743-40858762 None:intergenic 35.0%
GTTTATTGCTTACAACAGAC+AGG - chr1.2:40860080-40860099 MS.gene99003:intron 35.0%
TGTAAGCAATAAACGAAGCT+CGG + chr1.2:40860074-40860093 None:intergenic 35.0%
TGTTTGTTTGGTTGTTGCAA+GGG + chr1.2:40860513-40860532 None:intergenic 35.0%
TTGAATTTGCACCCAAGATT+TGG - chr1.2:40859088-40859107 MS.gene99003:intron 35.0%
! GGAAGTGATGATGATGAAAA+TGG - chr1.2:40859499-40859518 MS.gene99003:intron 35.0%
! TTGCAATGGATTTCAGTATG+AGG - chr1.2:40859416-40859435 MS.gene99003:intron 35.0%
! TTTGTTATCATCAGGTAGAC+CGG - chr1.2:40859297-40859316 MS.gene99003:intron 35.0%
!! TTGACTTGTTGGTGTTTGTT+TGG + chr1.2:40860525-40860544 None:intergenic 35.0%
!!! TTCTGAAGCTTTTCCTTGTT+GGG + chr1.2:40858798-40858817 None:intergenic 35.0%
AACCAAACTTCAACTTGACG+AGG + chr1.2:40859744-40859763 None:intergenic 40.0%
AATGAAGGTGAAGCTGAAAG+AGG - chr1.2:40859478-40859497 MS.gene99003:intron 40.0%
AGAAGGTCTAGTTGAATAGG+TGG + chr1.2:40858842-40858861 None:intergenic 40.0%
AGTTTCTTCTCAACCCAACA+AGG - chr1.2:40858782-40858801 MS.gene99003:CDS 40.0%
ATTGCTTACAACAGACAGGA+TGG - chr1.2:40860084-40860103 MS.gene99003:intron 40.0%
GATCTAAGAACAGGAGAGAA+AGG + chr1.2:40858866-40858885 None:intergenic 40.0%
GGAAGAAGGTCTAGTTGAAT+AGG + chr1.2:40858845-40858864 None:intergenic 40.0%
GTAGAAGCAAAAACTTGCAC+TGG - chr1.2:40859698-40859717 MS.gene99003:intron 40.0%
GTGTTTGTTTGGTTGTTGCA+AGG + chr1.2:40860514-40860533 None:intergenic 40.0%
GTTGAGAAGAAACTTGGTAG+TGG + chr1.2:40858776-40858795 None:intergenic 40.0%
TTCTGTGTCACCATTTGCAA+TGG - chr1.2:40859402-40859421 MS.gene99003:intron 40.0%
TTGCTTACAACAGACAGGAT+GGG - chr1.2:40860085-40860104 MS.gene99003:intron 40.0%
! AATAAACGAAGCTCGGTTTG+AGG + chr1.2:40860067-40860086 None:intergenic 40.0%
! ATGGAACTTGCTTTGAGCTT+AGG - chr1.2:40858701-40858720 MS.gene99003:CDS 40.0%
!! GAAACTTGGTAGTGGATCTT+TGG + chr1.2:40858768-40858787 None:intergenic 40.0%
!! TGGCAGTATTCTTGACTTGT+TGG + chr1.2:40860536-40860555 None:intergenic 40.0%
!! TGTTGGGTTGAGAAGAAACT+TGG + chr1.2:40858782-40858801 None:intergenic 40.0%
!!! CTTCTGAAGCTTTTCCTTGT+TGG + chr1.2:40858799-40858818 None:intergenic 40.0%
AAATGGTGACACAGAACTGC+TGG + chr1.2:40859398-40859417 None:intergenic 45.0%
AATGGTGACACAGAACTGCT+GGG + chr1.2:40859397-40859416 None:intergenic 45.0%
AGAAGGACACATAGTGAGAG+TGG + chr1.2:40860470-40860489 None:intergenic 45.0%
ATCATCAGGTAGACCGGTAA+GGG - chr1.2:40859303-40859322 MS.gene99003:intron 45.0%
GAACAGGAGAGAAAGGAAGA+AGG + chr1.2:40858859-40858878 None:intergenic 45.0%
GCTGGAAGTTGCATGTAGAA+TGG + chr1.2:40860445-40860464 None:intergenic 45.0%
GTTGCTACACGTTCACAAGA+AGG + chr1.2:40860487-40860506 None:intergenic 45.0%
TATCATCAGGTAGACCGGTA+AGG - chr1.2:40859302-40859321 MS.gene99003:intron 45.0%
TGGGCTTGAGCATTAGAGAA+TGG + chr1.2:40860556-40860575 None:intergenic 45.0%
! TGCACCCAAGATTTGGTACA+AGG - chr1.2:40859095-40859114 MS.gene99003:intron 45.0%
!! ACTTGGTAGTGGATCTTTGG+TGG + chr1.2:40858765-40858784 None:intergenic 45.0%
!! ATGAAGGAATCTGATGGGCT+TGG + chr1.2:40860597-40860616 None:intergenic 45.0%
!! ATGGGTGTTACTCATCATCG+CGG - chr1.2:40860103-40860122 MS.gene99003:intron 45.0%
!! CTTGGTAGTGGATCTTTGGT+GGG + chr1.2:40858764-40858783 None:intergenic 45.0%
!! GTCCTCGTCAAGTTGAAGTT+TGG - chr1.2:40859739-40859758 MS.gene99003:CDS 45.0%
!! TGAAGGAATCTGATGGGCTT+GGG + chr1.2:40860596-40860615 None:intergenic 45.0%
!!! ATAAAAAAAAGATATTTTTT+AGG + chr1.2:40859848-40859867 None:intergenic 5.0%
!!! TAAAAAAAAGATATTTTTTA+GGG + chr1.2:40859847-40859866 None:intergenic 5.0%
ATGGTGACACAGAACTGCTG+GGG + chr1.2:40859396-40859415 None:intergenic 50.0%
CACATAGTGAGAGTGGTAGC+TGG + chr1.2:40860463-40860482 None:intergenic 50.0%
GAATGGGGAGGTGAGAAGAA+GGG + chr1.2:40858895-40858914 None:intergenic 50.0%
GGGTGAGGAGATCTAAGAAC+AGG + chr1.2:40858875-40858894 None:intergenic 50.0%
TTACATGGTTGGGTGAGCCA+AGG + chr1.2:40858917-40858936 None:intergenic 50.0%
! TTCACGTCTAGCTCCCTTAC+CGG + chr1.2:40859319-40859338 None:intergenic 50.0%
ACTGCTGGGGGATGACAATG+AGG + chr1.2:40859383-40859402 None:intergenic 55.0%
ATGACCGAAGACGTCGACGA+CGG - chr1.2:40859355-40859374 MS.gene99003:intron 55.0%
CTTCTCACCTCCCCATTCCT+TGG - chr1.2:40858897-40858916 MS.gene99003:CDS 55.0%
GCTAGACGTGAACCGTTTCC+CGG - chr1.2:40859327-40859346 MS.gene99003:intron 55.0%
GGAATGGGGAGGTGAGAAGA+AGG + chr1.2:40858896-40858915 None:intergenic 55.0%
GGTTGGGTGAGCCAAGGAAT+GGG + chr1.2:40858911-40858930 None:intergenic 55.0%
GTTGGGTGAGCCAAGGAATG+GGG + chr1.2:40858910-40858929 None:intergenic 55.0%
TGGTGACACAGAACTGCTGG+GGG + chr1.2:40859395-40859414 None:intergenic 55.0%
TGGTTGGGTGAGCCAAGGAA+TGG + chr1.2:40858912-40858931 None:intergenic 55.0%
AGACGTGAACCGTTTCCCGG+CGG - chr1.2:40859330-40859349 MS.gene99003:intron 60.0%
GGCTTGGGCTTGAGCCTGAT+GGG + chr1.2:40860581-40860600 None:intergenic 60.0%
GGGAGGTGAGAAGAAGGGTG+AGG + chr1.2:40858890-40858909 None:intergenic 60.0%
TGCTCAAGCCCAAGCCCATC+AGG - chr1.2:40860564-40860583 MS.gene99003:CDS 60.0%
!! GGCTTGAGCCTGATGGGCTT+GGG + chr1.2:40860575-40860594 None:intergenic 60.0%
ACGTCTTCGGTCATCGCCGC+CGG + chr1.2:40859349-40859368 None:intergenic 65.0%
GGGCTTGGGCTTGAGCCTGA+TGG + chr1.2:40860582-40860601 None:intergenic 65.0%
GGGTGAGCCAAGGAATGGGG+AGG + chr1.2:40858907-40858926 None:intergenic 65.0%
! GGTGCCGTCGTCGACGTCTT+CGG + chr1.2:40859362-40859381 None:intergenic 65.0%
!! GGGCTTGAGCCTGATGGGCT+TGG + chr1.2:40860576-40860595 None:intergenic 65.0%
CGGTCATCGCCGCCGGGAAA+CGG + chr1.2:40859342-40859361 None:intergenic 70.0%
CGTCTTCGGTCATCGCCGCC+GGG + chr1.2:40859348-40859367 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 40858701 40860618 40858701 ID=MS.gene99003
chr1.2 mRNA 40858701 40860618 40858701 ID=MS.gene99003.t1;Parent=MS.gene99003
chr1.2 exon 40860387 40860618 40860387 ID=MS.gene99003.t1.exon1;Parent=MS.gene99003.t1
chr1.2 CDS 40860387 40860618 40860387 ID=cds.MS.gene99003.t1;Parent=MS.gene99003.t1
chr1.2 exon 40859713 40860008 40859713 ID=MS.gene99003.t1.exon2;Parent=MS.gene99003.t1
chr1.2 CDS 40859713 40860008 40859713 ID=cds.MS.gene99003.t1;Parent=MS.gene99003.t1
chr1.2 exon 40859538 40859617 40859538 ID=MS.gene99003.t1.exon3;Parent=MS.gene99003.t1
chr1.2 CDS 40859538 40859617 40859538 ID=cds.MS.gene99003.t1;Parent=MS.gene99003.t1
chr1.2 exon 40858701 40859001 40858701 ID=MS.gene99003.t1.exon4;Parent=MS.gene99003.t1
chr1.2 CDS 40858701 40859001 40858701 ID=cds.MS.gene99003.t1;Parent=MS.gene99003.t1
Gene Sequence

>MS.gene99003

ATGGAACTTGCTTTGAGCTTAGGTGACACACCAAAATTACCTATCTCTTTTCTTGACAAACCCACCAAAGATCCACTACCAAGTTTCTTCTCAACCCAACAAGGAAAAGCTTCAGAAGAAAAAAGAGGTTCTTCAGATCCACCTATTCAACTAGACCTTCTTCCTTTCTCTCCTGTTCTTAGATCTCCTCACCCTTCTTCTCACCTCCCCATTCCTTGGCTCACCCAACCATGTAATTTTCTATCTACTATAAATCCTTTTCTTTCCTCTAAATGGTTTTTATATATTTGTTTAATATCTAGATAAATTTGTTTAATTTTATTTTGTTTTTATAGATTTAATTTTATCTTTTTTGTTTTTGTTTTAAAGTGCTTGAAAATTGTGGGTTTGAATTTGCACCCAAGATTTGGTACAAGGTTTTGCATATTCTTGACTAATGTATATTGAAAAATATTTGTTATGAAAAGTCAATTTTTTTAATGGATCTAGGTTACTTCGAGATATTAGTCTTTGCAGTTACGCGCATGAATTCATATTGTTATCAAAAAAAATTTGAAAATATATTTAAAGTTGTATATACTCAATCATTTTTTGTTTTTGTTATCATCAGGTAGACCGGTAAGGGAGCTAGACGTGAACCGTTTCCCGGCGGCGATGACCGAAGACGTCGACGACGGCACCTCATTGTCATCCCCCAGCAGTTCTGTGTCACCATTTGCAATGGATTTCAGTATGAGGAATAACAATGCTGAATATGGAGGAAGAAACAAGAGAGAAAATGAAGGTGAAGCTGAAAGAGGAAGTGATGATGATGAAAATGGGTCAACTAGGAAGAAACTAAGACTCTCTAAAGATCAATCTGCTTTTCTTGAAGAAAGTTTCAAAGAACACACCACTCTTAATCCTGTAAATTTCTTATTTAATTTTTGTTTATTTACTTGTTTCTTCTTTTTAATTAAGGTTTATTTGTTAATTAATCATGTTTATTTATTTGCTTGTAGAAGCAAAAACTTGCACTGGCAAAACAGTTGAATCTTAGTCCTCGTCAAGTTGAAGTTTGGTTTCAAAATAGAAGAGCAAGGTGAGTCTTATCCTTCAATATTTTTGTTTTAATTTTTTATCTAGGTTTAAATGCTATTCATACCCTAAAAAATATCTTTTTTTTATGCTTTTAATCATTGAAAATATTATTTTATTTTAGTCCTCATAAATATATGTCATTTTGTCTCTTCAGGTAGAAATGAATTTTTCTAATATTTTATTTTAGAGTTTATTTTGAAGAATTAAAATCAAATAGTATGTATCCTAATAAGTTAGAATATGAATAATTTTTGTTTGAACTAAAACAATGATTGTGTTTAATCCTCAAACCGAGCTTCGTTTATTGCTTACAACAGACAGGATGGGTGTTACTCATCATCGCGGCCAGTATTTTTTTGTTTATTTTTTTTGCTTTGCCTTTTTTTCATGTAACCAAAAAAAACAAAACCATAAGATAAAGAAATCTAACACTACTAGAATTCTTTTGATATTTGTTGGTACCATTTACTTATCTATTTAAGTCATCTTTAATATTTTTTTTATTAAAGTTTTGTTTTGGAATGTATGATTTTGTAGGACAAAGCTAAAGCAAACAGAAGTGGATTGTGAGTATTTGAAGAGATGTTGTGAGACACTAACAGAAGAAAATAGAAGATTACAAAAAGAACTTCAAGAGCTTAGAGCTTTGAAAACTACTCAACCATTCTACATGCAACTTCCAGCTACCACTCTCACTATGTGTCCTTCTTGTGAACGTGTAGCAACAAACCCTTGCAACAACCAAACAAACACCAACAAGTCAAGAATACTGCCATTCTCTAATGCTCAAGCCCAAGCCCATCAGGCTCAAGCCCAAGCCCATCAGATTCCTTCATGA
Protein sequence

>MS.gene99003.t1

MELALSLGDTPKLPISFLDKPTKDPLPSFFSTQQGKASEEKRGSSDPPIQLDLLPFSPVLRSPHPSSHLPIPWLTQPCRPVRELDVNRFPAAMTEDVDDGTSLSSPSSSVSPFAMDFSMRNNNAEYGGRNKRENEGEAERGSDDDENGSTRKKLRLSKDQSAFLEESFKEHTTLNPKQKLALAKQLNLSPRQVEVWFQNRRARTKLKQTEVDCEYLKRCCETLTEENRRLQKELQELRALKTTQPFYMQLPATTLTMCPSCERVATNPCNNQTNTNKSRILPFSNAQAQAHQAQAQAHQIPS