AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09072


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09072.t1 MTR_8g086020 96.875 160 5 0 1 160 1 160 1.85e-114 321
MS.gene09072.t1 MTR_4g070860 52.448 143 62 3 15 155 14 152 9.57e-44 142
MS.gene09072.t1 MTR_1g022495 48.795 166 73 6 1 160 1 160 2.25e-41 136
MS.gene09072.t1 MTR_3g117120 51.064 141 64 2 15 155 14 149 1.02e-37 126
MS.gene09072.t1 MTR_3g112220 57.692 78 33 0 33 110 2 79 4.07e-25 92.8
MS.gene09072.t1 MTR_1g087040 40.141 142 74 5 1 140 1 133 8.61e-22 85.9
MS.gene09072.t1 MTR_8g015250 39.000 100 59 1 4 103 45 142 2.41e-15 70.1
MS.gene09072.t1 MTR_1g017570 40.426 94 53 1 26 119 51 141 5.12e-14 65.5
MS.gene09072.t1 MTR_7g029400 39.080 87 53 0 17 103 66 152 5.40e-14 66.6
MS.gene09072.t1 MTR_5g015090 38.393 112 59 4 2 110 32 136 7.52e-14 65.5
MS.gene09072.t1 MTR_4g097440 33.621 116 74 1 2 114 39 154 1.41e-13 65.1
MS.gene09072.t1 MTR_3g109310 31.132 106 73 0 9 114 100 205 1.88e-11 60.8
MS.gene09072.t1 MTR_6g016375 34.000 100 66 0 4 103 59 158 2.95e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09072.t1 AT1G75390 51.899 158 68 4 2 154 10 164 5.72e-46 148
MS.gene09072.t1 AT4G34590 50.725 138 59 3 31 160 23 159 2.33e-37 126
MS.gene09072.t1 AT2G18160 42.405 158 68 4 21 157 15 170 2.10e-32 114
MS.gene09072.t1 AT1G75390 54.082 98 43 1 2 99 10 105 6.61e-30 105
MS.gene09072.t1 AT3G62420 38.621 145 85 3 11 155 2 142 1.11e-27 100
MS.gene09072.t1 AT4G37730 38.235 102 52 3 13 103 171 272 1.23e-13 67.0
MS.gene09072.t1 AT3G30530 39.216 102 61 1 3 103 48 149 3.97e-12 61.2
MS.gene09072.t1 AT5G49450 45.122 82 45 0 33 114 14 95 4.04e-12 60.8
MS.gene09072.t1 AT1G13600 35.455 110 66 2 17 126 70 174 4.32e-12 61.6
MS.gene09072.t1 AT5G15830 34.426 122 77 1 13 131 52 173 6.77e-12 60.8
MS.gene09072.t1 AT2G04038 37.931 87 53 1 17 103 57 142 3.04e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTGAAAATGCTATTCTT+AGG 0.244925 4.4:-14068623 MS.gene09072:CDS
ATGATTGGTTGTTGATTAAC+AGG 0.261835 4.4:+14068462 None:intergenic
TTGATCTTCGTGCTGATTCT+CGG 0.312597 4.4:+14068763 None:intergenic
GTTCAATGATTGTGGATTCT+TGG 0.317045 4.4:-14068502 MS.gene09072:CDS
TGCTATTCTTAGGGTTCAAA+TGG 0.377563 4.4:-14068613 MS.gene09072:CDS
CCTGGTGGAACTTATTCATC+TGG 0.409672 4.4:-14068882 MS.gene09072:CDS
ATCTGAAAATGCTATTCTTA+GGG 0.417867 4.4:-14068622 MS.gene09072:CDS
GATGAAGAAACAGCAACATA+TGG 0.436530 4.4:-14068739 MS.gene09072:CDS
GATCAATTGTTCAATGATTG+TGG 0.455410 4.4:-14068510 MS.gene09072:CDS
TCTTCGTGCTGATTCTCGGT+TGG 0.456704 4.4:+14068767 None:intergenic
TGATCAGAAGAAGCCATGAT+TGG 0.513873 4.4:+14068447 None:intergenic
TGTTAATCAACAACCAATCA+TGG 0.519818 4.4:-14068460 MS.gene09072:CDS
GAGAATCAGCACGAAGATCA+AGG 0.528035 4.4:-14068761 MS.gene09072:CDS
GACCTTGATATGGCTTCTCC+TGG 0.533330 4.4:-14068900 None:intergenic
CTTCAAAACTCTGGATCAGA+GGG 0.550324 4.4:-14068849 MS.gene09072:CDS
ACAAGCTCTCTTCAAAACTC+TGG 0.550443 4.4:-14068858 MS.gene09072:CDS
CTTGATATGGCTTCTCCTGG+TGG 0.579058 4.4:-14068897 None:intergenic
TCTTCAAAACTCTGGATCAG+AGG 0.579120 4.4:-14068850 MS.gene09072:CDS
GGGAGATCATAATAACATGC+AGG 0.579180 4.4:-14068829 MS.gene09072:CDS
AAATGGCTGAACTTAGCCAC+AGG 0.585823 4.4:-14068596 MS.gene09072:CDS
CCAGATGAATAAGTTCCACC+AGG 0.607477 4.4:+14068882 None:intergenic
ATTGTGGATTCTTGGACACA+TGG 0.610654 4.4:-14068494 MS.gene09072:CDS
ACATAACAGATCAAAAGAAG+AGG 0.628528 4.4:-14068803 MS.gene09072:CDS
ATTCAAATAAGCGCAAACGT+TGG 0.641513 4.4:-14068675 MS.gene09072:CDS
CATTAAGTGATTGCAACCTG+TGG 0.653582 4.4:+14068580 None:intergenic
TACTCAGATGTACCTGAACG+TGG 0.654069 4.4:-14068646 MS.gene09072:CDS
TGATTGGTTGTTGATTAACA+GGG 0.670028 4.4:+14068463 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATCTGAAAATGCTATTCTT+AGG - chr4.4:14068699-14068718 MS.gene09072:CDS 25.0%
! ATCTGAAAATGCTATTCTTA+GGG - chr4.4:14068700-14068719 MS.gene09072:CDS 25.0%
ACATAACAGATCAAAAGAAG+AGG - chr4.4:14068519-14068538 MS.gene09072:CDS 30.0%
GATCAATTGTTCAATGATTG+TGG - chr4.4:14068812-14068831 MS.gene09072:CDS 30.0%
TGTTAATCAACAACCAATCA+TGG - chr4.4:14068862-14068881 MS.gene09072:CDS 30.0%
! ATGATTGGTTGTTGATTAAC+AGG + chr4.4:14068863-14068882 None:intergenic 30.0%
! TGATTGGTTGTTGATTAACA+GGG + chr4.4:14068862-14068881 None:intergenic 30.0%
!!! ATCAAGTAACTCACTTTTTC+AGG - chr4.4:14068784-14068803 MS.gene09072:CDS 30.0%
!!! CTTTTTTCAGCTGTTCAATT+TGG + chr4.4:14068622-14068641 None:intergenic 30.0%
ATTCAAATAAGCGCAAACGT+TGG - chr4.4:14068647-14068666 MS.gene09072:CDS 35.0%
GATGAAGAAACAGCAACATA+TGG - chr4.4:14068583-14068602 MS.gene09072:CDS 35.0%
GTTCAATGATTGTGGATTCT+TGG - chr4.4:14068820-14068839 MS.gene09072:CDS 35.0%
TGCTATTCTTAGGGTTCAAA+TGG - chr4.4:14068709-14068728 MS.gene09072:CDS 35.0%
ACAAGCTCTCTTCAAAACTC+TGG - chr4.4:14068464-14068483 MS.gene09072:CDS 40.0%
ATTGTGGATTCTTGGACACA+TGG - chr4.4:14068828-14068847 MS.gene09072:CDS 40.0%
CATTAAGTGATTGCAACCTG+TGG + chr4.4:14068745-14068764 None:intergenic 40.0%
CTTCAAAACTCTGGATCAGA+GGG - chr4.4:14068473-14068492 MS.gene09072:CDS 40.0%
GGGAGATCATAATAACATGC+AGG - chr4.4:14068493-14068512 MS.gene09072:CDS 40.0%
TCTTCAAAACTCTGGATCAG+AGG - chr4.4:14068472-14068491 MS.gene09072:CDS 40.0%
TGATCAGAAGAAGCCATGAT+TGG + chr4.4:14068878-14068897 None:intergenic 40.0%
! CATTTTCAGATTCCACGTTC+AGG + chr4.4:14068691-14068710 None:intergenic 40.0%
!! TTGATCTTCGTGCTGATTCT+CGG + chr4.4:14068562-14068581 None:intergenic 40.0%
AAATGGCTGAACTTAGCCAC+AGG - chr4.4:14068726-14068745 MS.gene09072:CDS 45.0%
CCAGATGAATAAGTTCCACC+AGG + chr4.4:14068443-14068462 None:intergenic 45.0%
CCTGGTGGAACTTATTCATC+TGG - chr4.4:14068440-14068459 MS.gene09072:CDS 45.0%
GAGAATCAGCACGAAGATCA+AGG - chr4.4:14068561-14068580 MS.gene09072:CDS 45.0%
TACTCAGATGTACCTGAACG+TGG - chr4.4:14068676-14068695 MS.gene09072:CDS 45.0%
!! TCTTCGTGCTGATTCTCGGT+TGG + chr4.4:14068558-14068577 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 14068431 14068913 14068431 ID=MS.gene09072
chr4.4 mRNA 14068431 14068913 14068431 ID=MS.gene09072.t1;Parent=MS.gene09072
chr4.4 exon 14068431 14068913 14068431 ID=MS.gene09072.t1.exon1;Parent=MS.gene09072.t1
chr4.4 CDS 14068431 14068913 14068431 ID=cds.MS.gene09072.t1;Parent=MS.gene09072.t1
Gene Sequence

>MS.gene09072

ATGGCTTCTCCTGGTGGAACTTATTCATCTGGCACAAGCTCTCTTCAAAACTCTGGATCAGAGGGAGATCATAATAACATGCAGGTGAACATAACAGATCAAAAGAAGAGGAAGAGAATGCAATCCAACCGAGAATCAGCACGAAGATCAAGGATGAAGAAACAGCAACATATGGAAGATCTAAGCAACCAAATTGAACAGCTGAAAAAAGAGAACATTCAAATAAGCGCAAACGTTGGAGTAACTACTCAGATGTACCTGAACGTGGAATCTGAAAATGCTATTCTTAGGGTTCAAATGGCTGAACTTAGCCACAGGTTGCAATCACTTAATGATATTATTCATTACATTGAATCAAGTAACTCACTTTTTCAGGAGACAGATCAATTGTTCAATGATTGTGGATTCTTGGACACATGGAACACTTTCCCTGTTAATCAACAACCAATCATGGCTTCTTCTGATCATATGTTAATGTATTGA

Protein sequence

>MS.gene09072.t1

MASPGGTYSSGTSSLQNSGSEGDHNNMQVNITDQKKRKRMQSNRESARRSRMKKQQHMEDLSNQIEQLKKENIQISANVGVTTQMYLNVESENAILRVQMAELSHRLQSLNDIIHYIESSNSLFQETDQLFNDCGFLDTWNTFPVNQQPIMASSDHMLMY