Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59053.t1 | XP_003609290.1 | 97.1 | 170 | 2 | 3 | 1 | 169 | 1 | 168 | 3.50E-87 | 330.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59053.t1 | G7JCV5 | 97.1 | 170 | 2 | 3 | 1 | 169 | 1 | 168 | 2.5e-87 | 330.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene59053 | -0.814054 | 1.87E-51 | -1.69E-46 |
| MS.gene049217 | MS.gene59053 | -0.826182 | 3.12E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene59053 | -0.807504 | 4.89E-50 | -1.69E-46 |
| MS.gene049281 | MS.gene59053 | 0.825778 | 3.89E-54 | -1.69E-46 |
| MS.gene049285 | MS.gene59053 | -0.817983 | 2.48E-52 | -1.69E-46 |
| MS.gene049627 | MS.gene59053 | -0.825887 | 3.67E-54 | -1.69E-46 |
| MS.gene05010 | MS.gene59053 | -0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene050163 | MS.gene59053 | -0.807205 | 5.66E-50 | -1.69E-46 |
| MS.gene050165 | MS.gene59053 | -0.806589 | 7.64E-50 | -1.69E-46 |
| MS.gene050381 | MS.gene59053 | 0.835803 | 1.36E-56 | -1.69E-46 |
| MS.gene050504 | MS.gene59053 | -0.83052 | 2.80E-55 | -1.69E-46 |
| MS.gene050612 | MS.gene59053 | -0.811281 | 7.57E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene59053 | -0.81106 | 8.46E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene59053 | -0.810649 | 1.04E-50 | -1.69E-46 |
| MS.gene051694 | MS.gene59053 | -0.811244 | 7.71E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene59053 | 0.83317 | 6.22E-56 | -1.69E-46 |
| MS.gene052164 | MS.gene59053 | -0.820542 | 6.49E-53 | -1.69E-46 |
| MS.gene05234 | MS.gene59053 | 0.815612 | 8.46E-52 | -1.69E-46 |
| MS.gene052482 | MS.gene59053 | -0.821891 | 3.17E-53 | -1.69E-46 |
| MS.gene052486 | MS.gene59053 | -0.832219 | 1.07E-55 | -1.69E-46 |
| MS.gene052966 | MS.gene59053 | -0.80056 | 1.36E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene59053 | 0.819156 | 1.35E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene59053 | -0.803796 | 2.94E-49 | -1.69E-46 |
| MS.gene054616 | MS.gene59053 | 0.808651 | 2.79E-50 | -1.69E-46 |
| MS.gene054954 | MS.gene59053 | -0.818324 | 2.08E-52 | -1.69E-46 |
| MS.gene054959 | MS.gene59053 | 0.814897 | 1.22E-51 | -1.69E-46 |
| MS.gene055202 | MS.gene59053 | 0.814801 | 1.28E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene59053 | -0.810501 | 1.12E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene59053 | -0.825329 | 4.97E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene59053 | -0.825018 | 5.89E-54 | -1.69E-46 |
| MS.gene05589 | MS.gene59053 | -0.808961 | 2.39E-50 | -1.69E-46 |
| MS.gene055941 | MS.gene59053 | -0.800062 | 1.72E-48 | -1.69E-46 |
| MS.gene056347 | MS.gene59053 | 0.811156 | 8.06E-51 | -1.69E-46 |
| MS.gene056409 | MS.gene59053 | -0.809569 | 1.77E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene59053 | -0.816772 | 4.65E-52 | -1.69E-46 |
| MS.gene056550 | MS.gene59053 | 0.823376 | 1.43E-53 | -1.69E-46 |
| MS.gene056610 | MS.gene59053 | -0.807722 | 4.40E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene59053 | 0.833823 | 4.27E-56 | -1.69E-46 |
| MS.gene057831 | MS.gene59053 | -0.811981 | 5.33E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene59053 | -0.81748 | 3.23E-52 | -1.69E-46 |
| MS.gene058419 | MS.gene59053 | -0.809045 | 2.30E-50 | -1.69E-46 |
| MS.gene058773 | MS.gene59053 | -0.827605 | 1.43E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene59053 | -0.82738 | 1.62E-54 | -1.69E-46 |
| MS.gene059023 | MS.gene59053 | -0.831559 | 1.56E-55 | -1.69E-46 |
| MS.gene059677 | MS.gene59053 | -0.816244 | 6.11E-52 | -1.69E-46 |
| MS.gene059718 | MS.gene59053 | -0.820132 | 8.06E-53 | -1.69E-46 |
| MS.gene059801 | MS.gene59053 | -0.820522 | 6.56E-53 | -1.69E-46 |
| MS.gene059902 | MS.gene59053 | -0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene59053 | 0.840666 | 7.58E-58 | -1.69E-46 |
| MS.gene060703 | MS.gene59053 | -0.817792 | 2.74E-52 | -1.69E-46 |
| MS.gene060762 | MS.gene59053 | -0.823448 | 1.38E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene59053 | -0.838683 | 2.49E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59053.t1 | MTR_4g114080 | 97.059 | 170 | 2 | 3 | 1 | 169 | 1 | 168 | 4.72e-117 | 328 |
| MS.gene59053.t1 | MTR_4g011020 | 66.026 | 156 | 51 | 2 | 1 | 156 | 5 | 158 | 2.31e-76 | 226 |
| MS.gene59053.t1 | MTR_2g103490 | 60.947 | 169 | 61 | 4 | 1 | 168 | 5 | 169 | 3.42e-67 | 202 |
| MS.gene59053.t1 | MTR_2g103460 | 57.738 | 168 | 66 | 4 | 1 | 167 | 5 | 168 | 1.90e-62 | 190 |
| MS.gene59053.t1 | MTR_2g005880 | 60.432 | 139 | 53 | 1 | 4 | 142 | 2 | 138 | 2.43e-57 | 177 |
| MS.gene59053.t1 | MTR_5g021610 | 57.534 | 146 | 60 | 1 | 2 | 145 | 22 | 167 | 1.23e-56 | 177 |
| MS.gene59053.t1 | MTR_8g076160 | 55.556 | 144 | 62 | 1 | 4 | 145 | 12 | 155 | 7.83e-55 | 172 |
| MS.gene59053.t1 | MTR_0003s0630 | 52.143 | 140 | 65 | 2 | 3 | 140 | 32 | 171 | 3.28e-50 | 160 |
| MS.gene59053.t1 | MTR_1g115170 | 54.730 | 148 | 62 | 4 | 20 | 166 | 1 | 144 | 2.03e-49 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59053.t1 | AT1G80160 | 63.804 | 163 | 57 | 2 | 2 | 164 | 6 | 166 | 2.35e-73 | 218 |
| MS.gene59053.t1 | AT1G80160 | 63.804 | 163 | 57 | 2 | 2 | 164 | 47 | 207 | 8.78e-73 | 218 |
| MS.gene59053.t1 | AT1G15380 | 62.130 | 169 | 59 | 4 | 2 | 167 | 6 | 172 | 5.90e-71 | 212 |
| MS.gene59053.t1 | AT1G15380 | 62.130 | 169 | 59 | 4 | 2 | 167 | 6 | 172 | 5.90e-71 | 212 |
| MS.gene59053.t1 | AT2G28420 | 51.852 | 135 | 63 | 2 | 7 | 140 | 18 | 151 | 2.73e-51 | 162 |
| MS.gene59053.t1 | AT1G80160 | 62.069 | 116 | 42 | 2 | 49 | 164 | 27 | 140 | 4.48e-45 | 145 |
Find 29 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATTCTTGGTCATACTTTC+TGG | 0.158615 | 8.4:-13869882 | None:intergenic |
| TTGATTAGGTTGTTTAATTA+TGG | 0.210559 | 8.4:+13869825 | MS.gene59053:intron |
| AAGAACAGAGTAGAGGAAAA+TGG | 0.294782 | 8.4:+13870094 | MS.gene59053:CDS |
| ATTAATGTGCACATTATTCT+TGG | 0.304684 | 8.4:-13869895 | None:intergenic |
| CAAATTGTGTTCTCTGATAA+AGG | 0.332337 | 8.4:-13870199 | None:intergenic |
| GATATACTTGGAAAGAAATA+TGG | 0.341030 | 8.4:-13869929 | None:intergenic |
| TTCTCTGATAAAGGTACAAT+TGG | 0.358152 | 8.4:-13870190 | None:intergenic |
| AAAGAAATATGGTTGTCCTT+TGG | 0.368432 | 8.4:-13869918 | None:intergenic |
| AAGGAGGTAGGTCTCTTAAT+AGG | 0.373287 | 8.4:-13869680 | None:intergenic |
| CTTTATCAGAGAACACAATT+TGG | 0.412500 | 8.4:+13870200 | MS.gene59053:CDS |
| TTTCTGGATCATCATCTGAT+TGG | 0.413129 | 8.4:-13869866 | None:intergenic |
| TCGTTGCTAGTGTGAAAGTA+TGG | 0.421471 | 8.4:+13870027 | MS.gene59053:intron |
| TACGTACCATGCACCATTGA+AGG | 0.464268 | 8.4:-13869705 | None:intergenic |
| ATCATTGAATCACATCTCAT+TGG | 0.477448 | 8.4:+13869601 | MS.gene59053:CDS |
| ATTCAATGATTTGAGTTGTA+GGG | 0.480431 | 8.4:-13869588 | None:intergenic |
| ATTTCAATCATAGTGCCATC+TGG | 0.481430 | 8.4:-13870148 | None:intergenic |
| TCATAGTGCCATCTGGATCA+TGG | 0.483510 | 8.4:-13870141 | None:intergenic |
| AGGTTGTTTAATTATGGCAT+TGG | 0.497038 | 8.4:+13869831 | MS.gene59053:intron |
| CCTACCTCCTTAGCCTTCAA+TGG | 0.497853 | 8.4:+13869692 | MS.gene59053:CDS |
| GATTCAATGATTTGAGTTGT+AGG | 0.509824 | 8.4:-13869589 | None:intergenic |
| GTGTGAAAGTATGGCAGTAG+TGG | 0.540760 | 8.4:+13870036 | MS.gene59053:intron |
| TTCAATGATTTGAGTTGTAG+GGG | 0.553026 | 8.4:-13869587 | None:intergenic |
| CCATGCACCATTGAAGGCTA+AGG | 0.575830 | 8.4:-13869699 | None:intergenic |
| GTATGTGAAGAACAGAGTAG+AGG | 0.586484 | 8.4:+13870087 | MS.gene59053:CDS |
| CCATTGAAGGCTAAGGAGGT+AGG | 0.593613 | 8.4:-13869692 | None:intergenic |
| TTGTTCTTCCATGATCCAGA+TGG | 0.596741 | 8.4:+13870133 | MS.gene59053:CDS |
| CCTTAGCCTTCAATGGTGCA+TGG | 0.610902 | 8.4:+13869699 | MS.gene59053:CDS |
| TAATGTGCACATTAATCCAA+AGG | 0.618094 | 8.4:+13869902 | MS.gene59053:CDS |
| TGCACCATTGAAGGCTAAGG+AGG | 0.624890 | 8.4:-13869696 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATTTACACAAATAGA+GGG | - | chr8.4:13869960-13869979 | None:intergenic | 15.0% |
| !! | AAATCACATAATAAGTATAT+AGG | - | chr8.4:13870003-13870022 | None:intergenic | 15.0% |
| !!! | ATAACTTATGTTTACATTTT+TGG | + | chr8.4:13869801-13869820 | MS.gene59053:intron | 15.0% |
| !! | AACATAAGTTATTCTAACAA+TGG | - | chr8.4:13869793-13869812 | None:intergenic | 20.0% |
| !! | GAAATAATTTACACAAATAG+AGG | - | chr8.4:13869961-13869980 | None:intergenic | 20.0% |
| !!! | TTGATTAGGTTGTTTAATTA+TGG | + | chr8.4:13869825-13869844 | MS.gene59053:intron | 20.0% |
| !!! | TTTACATTTTTGGTTTGATT+AGG | + | chr8.4:13869811-13869830 | MS.gene59053:intron | 20.0% |
| ! | ATTAATGTGCACATTATTCT+TGG | - | chr8.4:13869898-13869917 | None:intergenic | 25.0% |
| ! | GATATACTTGGAAAGAAATA+TGG | - | chr8.4:13869932-13869951 | None:intergenic | 25.0% |
| !!! | ATTCAATGATTTGAGTTGTA+GGG | - | chr8.4:13869591-13869610 | None:intergenic | 25.0% |
| AAAGAAATATGGTTGTCCTT+TGG | - | chr8.4:13869921-13869940 | None:intergenic | 30.0% | |
| AGGTTGTTTAATTATGGCAT+TGG | + | chr8.4:13869831-13869850 | MS.gene59053:intron | 30.0% | |
| ATCATTGAATCACATCTCAT+TGG | + | chr8.4:13869601-13869620 | MS.gene59053:CDS | 30.0% | |
| CAAATTGTGTTCTCTGATAA+AGG | - | chr8.4:13870202-13870221 | None:intergenic | 30.0% | |
| CTTTATCAGAGAACACAATT+TGG | + | chr8.4:13870200-13870219 | MS.gene59053:CDS | 30.0% | |
| TAATGTGCACATTAATCCAA+AGG | + | chr8.4:13869902-13869921 | MS.gene59053:CDS | 30.0% | |
| TTATTCTTGGTCATACTTTC+TGG | - | chr8.4:13869885-13869904 | None:intergenic | 30.0% | |
| ! | GATTTTTACGTCAATGTTCT+TGG | + | chr8.4:13869650-13869669 | MS.gene59053:CDS | 30.0% |
| ! | TTCTCTGATAAAGGTACAAT+TGG | - | chr8.4:13870193-13870212 | None:intergenic | 30.0% |
| !! | GATTCAATGATTTGAGTTGT+AGG | - | chr8.4:13869592-13869611 | None:intergenic | 30.0% |
| !! | TTCAATGATTTGAGTTGTAG+GGG | - | chr8.4:13869590-13869609 | None:intergenic | 30.0% |
| AAGAACAGAGTAGAGGAAAA+TGG | + | chr8.4:13870094-13870113 | MS.gene59053:CDS | 35.0% | |
| ATTTCAATCATAGTGCCATC+TGG | - | chr8.4:13870151-13870170 | None:intergenic | 35.0% | |
| TTTCTGGATCATCATCTGAT+TGG | - | chr8.4:13869869-13869888 | None:intergenic | 35.0% | |
| AAGGAGGTAGGTCTCTTAAT+AGG | - | chr8.4:13869683-13869702 | None:intergenic | 40.0% | |
| GTATGTGAAGAACAGAGTAG+AGG | + | chr8.4:13870087-13870106 | MS.gene59053:CDS | 40.0% | |
| TAGAGGGAGAGAGATATACT+TGG | - | chr8.4:13869944-13869963 | None:intergenic | 40.0% | |
| TCGTTGCTAGTGTGAAAGTA+TGG | + | chr8.4:13870027-13870046 | MS.gene59053:intron | 40.0% | |
| TTGTTCTTCCATGATCCAGA+TGG | + | chr8.4:13870133-13870152 | MS.gene59053:CDS | 40.0% | |
| TACGTACCATGCACCATTGA+AGG | - | chr8.4:13869708-13869727 | None:intergenic | 45.0% | |
| TCATAGTGCCATCTGGATCA+TGG | - | chr8.4:13870144-13870163 | None:intergenic | 45.0% | |
| ! | GTGTGAAAGTATGGCAGTAG+TGG | + | chr8.4:13870036-13870055 | MS.gene59053:intron | 45.0% |
| CCATGCACCATTGAAGGCTA+AGG | - | chr8.4:13869702-13869721 | None:intergenic | 50.0% | |
| CCATTGAAGGCTAAGGAGGT+AGG | - | chr8.4:13869695-13869714 | None:intergenic | 50.0% | |
| TGCACCATTGAAGGCTAAGG+AGG | - | chr8.4:13869699-13869718 | None:intergenic | 50.0% | |
| ! | CCTACCTCCTTAGCCTTCAA+TGG | + | chr8.4:13869692-13869711 | MS.gene59053:CDS | 50.0% |
| ! | CCTTAGCCTTCAATGGTGCA+TGG | + | chr8.4:13869699-13869718 | MS.gene59053:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 13869578 | 13870291 | 13869578 | ID=MS.gene59053 |
| chr8.4 | mRNA | 13869578 | 13870291 | 13869578 | ID=MS.gene59053.t1;Parent=MS.gene59053 |
| chr8.4 | exon | 13869578 | 13869720 | 13869578 | ID=MS.gene59053.t1.exon1;Parent=MS.gene59053.t1 |
| chr8.4 | CDS | 13869578 | 13869720 | 13869578 | ID=cds.MS.gene59053.t1;Parent=MS.gene59053.t1 |
| chr8.4 | exon | 13869833 | 13869944 | 13869833 | ID=MS.gene59053.t1.exon2;Parent=MS.gene59053.t1 |
| chr8.4 | CDS | 13869833 | 13869944 | 13869833 | ID=cds.MS.gene59053.t1;Parent=MS.gene59053.t1 |
| chr8.4 | exon | 13870037 | 13870291 | 13870037 | ID=MS.gene59053.t1.exon3;Parent=MS.gene59053.t1 |
| chr8.4 | CDS | 13870037 | 13870291 | 13870037 | ID=cds.MS.gene59053.t1;Parent=MS.gene59053.t1 |
| Gene Sequence |
| Protein sequence |
