Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074202.t1 | XP_013451322.1 | 92.5 | 67 | 4 | 1 | 18 | 84 | 65 | 130 | 2.90E-26 | 127.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074202.t1 | A0A072U6V3 | 92.5 | 67 | 4 | 1 | 18 | 84 | 65 | 130 | 2.1e-26 | 127.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene074202 | 0.836361 | 9.79E-57 | -1.69E-46 |
| MS.gene049404 | MS.gene074202 | 0.808104 | 3.65E-50 | -1.69E-46 |
| MS.gene049669 | MS.gene074202 | 0.827139 | 1.84E-54 | -1.69E-46 |
| MS.gene050026 | MS.gene074202 | 0.81921 | 1.31E-52 | -1.69E-46 |
| MS.gene050442 | MS.gene074202 | 0.800158 | 1.64E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene074202 | 0.835848 | 1.32E-56 | -1.69E-46 |
| MS.gene050953 | MS.gene074202 | 0.840418 | 8.80E-58 | -1.69E-46 |
| MS.gene051247 | MS.gene074202 | 0.816264 | 6.05E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene074202 | 0.816102 | 6.57E-52 | -1.69E-46 |
| MS.gene052500 | MS.gene074202 | 0.804518 | 2.08E-49 | -1.69E-46 |
| MS.gene052502 | MS.gene074202 | 0.800839 | 1.19E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene074202 | 0.817786 | 2.75E-52 | -1.69E-46 |
| MS.gene052582 | MS.gene074202 | 0.81112 | 8.21E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene074202 | 0.802691 | 4.98E-49 | -1.69E-46 |
| MS.gene052586 | MS.gene074202 | 0.804892 | 1.74E-49 | -1.69E-46 |
| MS.gene052826 | MS.gene074202 | 0.803212 | 3.88E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene074202 | 0.821327 | 4.28E-53 | -1.69E-46 |
| MS.gene053006 | MS.gene074202 | 0.852922 | 3.36E-61 | -1.69E-46 |
| MS.gene053082 | MS.gene074202 | 0.816577 | 5.15E-52 | -1.69E-46 |
| MS.gene05308 | MS.gene074202 | 0.81077 | 9.77E-51 | -1.69E-46 |
| MS.gene053166 | MS.gene074202 | 0.814801 | 1.28E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene074202 | 0.80327 | 3.78E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene074202 | 0.820315 | 7.32E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene074202 | 0.809304 | 2.02E-50 | -1.69E-46 |
| MS.gene054405 | MS.gene074202 | 0.832324 | 1.01E-55 | -1.69E-46 |
| MS.gene054576 | MS.gene074202 | 0.815353 | 9.66E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene074202 | 0.818652 | 1.75E-52 | -1.69E-46 |
| MS.gene054657 | MS.gene074202 | 0.802114 | 6.54E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene074202 | 0.80285 | 4.61E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene074202 | 0.812042 | 5.17E-51 | -1.69E-46 |
| MS.gene055586 | MS.gene074202 | 0.813181 | 2.91E-51 | -1.69E-46 |
| MS.gene055623 | MS.gene074202 | 0.825385 | 4.82E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene074202 | 0.817819 | 2.71E-52 | -1.69E-46 |
| MS.gene055778 | MS.gene074202 | 0.82275 | 2.00E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene074202 | 0.817782 | 2.76E-52 | -1.69E-46 |
| MS.gene055841 | MS.gene074202 | 0.822646 | 2.12E-53 | -1.69E-46 |
| MS.gene056162 | MS.gene074202 | 0.817932 | 2.55E-52 | -1.69E-46 |
| MS.gene056174 | MS.gene074202 | 0.823145 | 1.62E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene074202 | 0.809913 | 1.50E-50 | -1.69E-46 |
| MS.gene056373 | MS.gene074202 | 0.81408 | 1.85E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene074202 | 0.810621 | 1.05E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene074202 | 0.801983 | 6.96E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene074202 | 0.833356 | 5.59E-56 | -1.69E-46 |
| MS.gene057414 | MS.gene074202 | 0.80431 | 2.30E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene074202 | 0.800093 | 1.69E-48 | -1.69E-46 |
| MS.gene057932 | MS.gene074202 | 0.805415 | 1.35E-49 | -1.69E-46 |
| MS.gene058146 | MS.gene074202 | 0.803598 | 3.23E-49 | -1.69E-46 |
| MS.gene058279 | MS.gene074202 | 0.801891 | 7.27E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene074202 | 0.813035 | 3.14E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene074202 | 0.811822 | 5.78E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene074202 | 0.815938 | 7.15E-52 | -1.69E-46 |
| MS.gene060011 | MS.gene074202 | 0.80012 | 1.67E-48 | -1.69E-46 |
| MS.gene060167 | MS.gene074202 | 0.80236 | 5.82E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene074202 | 0.82208 | 2.87E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene074202 | 0.809098 | 2.24E-50 | -1.69E-46 |
| MS.gene060365 | MS.gene074202 | 0.841218 | 5.43E-58 | -1.69E-46 |
| MS.gene060429 | MS.gene074202 | 0.814325 | 1.63E-51 | -1.69E-46 |
| MS.gene060521 | MS.gene074202 | 0.822697 | 2.06E-53 | -1.69E-46 |
| MS.gene060879 | MS.gene074202 | 0.817411 | 3.34E-52 | -1.69E-46 |
| MS.gene060918 | MS.gene074202 | 0.80114 | 1.04E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074202.t1 | MTR_6g018800 | 92.537 | 67 | 4 | 1 | 18 | 84 | 65 | 130 | 4.78e-40 | 128 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAAGCTTTGATTCGAAAA+AGG | 0.284593 | 6.2:-14946942 | MS.gene074202:CDS |
| TTTCCTCTCCCACCTCTCCT+TGG | 0.409307 | 6.2:+14945820 | None:intergenic |
| AAAGAAGCCAAAATTGCATT+GGG | 0.411282 | 6.2:-14946891 | MS.gene074202:CDS |
| CAAAGAAGCCAAAATTGCAT+TGG | 0.485599 | 6.2:-14946892 | MS.gene074202:CDS |
| AGCTTTCACAATTGTTCAAG+AGG | 0.501859 | 6.2:+14946957 | None:intergenic |
| GGCTTGACACCAAGGAGAGG+TGG | 0.527330 | 6.2:-14945829 | MS.gene074202:CDS |
| ACACCAAGGAGAGGTGGGAG+AGG | 0.533494 | 6.2:-14945823 | MS.gene074202:CDS |
| AGCAAGAACAGTGGAGCTCA+AGG | 0.552731 | 6.2:-14947007 | MS.gene074202:CDS |
| GTGATATGATATCTACATGT+TGG | 0.574074 | 6.2:+14945796 | None:intergenic |
| GCTTGACACCAAGGAGAGGT+GGG | 0.599728 | 6.2:-14945828 | MS.gene074202:CDS |
| AGAAGCCAAAATTGCATTGG+GGG | 0.610391 | 6.2:-14946889 | MS.gene074202:intron |
| CATTTGCAGGCTTGACACCA+AGG | 0.630484 | 6.2:-14945837 | MS.gene074202:intron |
| GCAGGCTTGACACCAAGGAG+AGG | 0.644830 | 6.2:-14945832 | MS.gene074202:intron |
| GATGTATATAGCAAGAACAG+TGG | 0.667111 | 6.2:-14947016 | MS.gene074202:CDS |
| AAGAAGCCAAAATTGCATTG+GGG | 0.668833 | 6.2:-14946890 | MS.gene074202:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTATGTTATTCATGTATA+TGG | - | chr6.2:14946969-14946988 | MS.gene074202:CDS | 15.0% |
| !! | ATCAGAAAATTATATATCCA+CGG | - | chr6.2:14946107-14946126 | MS.gene074202:intron | 20.0% |
| !! | GATTACTATAAACACATTTA+AGG | + | chr6.2:14946788-14946807 | None:intergenic | 20.0% |
| !!! | TGATTTTCTCAAATTATTAG+CGG | + | chr6.2:14946851-14946870 | None:intergenic | 20.0% |
| ! | ATATTACTATCTATGAAGCA+CGG | + | chr6.2:14946944-14946963 | None:intergenic | 25.0% |
| ! | TCTAACTCTACTTTAAATGA+AGG | - | chr6.2:14946027-14946046 | MS.gene074202:intron | 25.0% |
| ! | TTCTGTGTAATTATATGTGT+CGG | - | chr6.2:14946876-14946895 | MS.gene074202:intron | 25.0% |
| ! | TTTATGAGTAAATCATGTGA+AGG | + | chr6.2:14946208-14946227 | None:intergenic | 25.0% |
| !!! | TATTTTGTGTGTTCTGAATT+GGG | - | chr6.2:14945811-14945830 | MS.gene074202:CDS | 25.0% |
| AAAGAAGCCAAAATTGCATT+GGG | - | chr6.2:14945996-14946015 | MS.gene074202:intron | 30.0% | |
| CGATTATGGATCACATAAAA+CGG | + | chr6.2:14946440-14946459 | None:intergenic | 30.0% | |
| TGAGCTTAGGATATTTACAT+GGG | - | chr6.2:14946489-14946508 | MS.gene074202:intron | 30.0% | |
| TGCAACATATTGTCAAATAG+CGG | - | chr6.2:14946386-14946405 | MS.gene074202:intron | 30.0% | |
| TTACATGGGATTTCAGTAAA+TGG | - | chr6.2:14946503-14946522 | MS.gene074202:intron | 30.0% | |
| ! | AACTGTTAAGTTTTTATCCG+TGG | + | chr6.2:14946127-14946146 | None:intergenic | 30.0% |
| ! | TGAAAGCTTTGATTCGAAAA+AGG | - | chr6.2:14945945-14945964 | MS.gene074202:intron | 30.0% |
| !! | AAATGGAAGTATCAGAAATG+AGG | - | chr6.2:14946520-14946539 | MS.gene074202:intron | 30.0% |
| !! | GTATTTTGTGTGTTCTGAAT+TGG | - | chr6.2:14945810-14945829 | MS.gene074202:CDS | 30.0% |
| AAGAAGCCAAAATTGCATTG+GGG | - | chr6.2:14945997-14946016 | MS.gene074202:intron | 35.0% | |
| ACGATAATGATCACTGCAAA+CGG | - | chr6.2:14946237-14946256 | MS.gene074202:intron | 35.0% | |
| AGCTTTCACAATTGTTCAAG+AGG | + | chr6.2:14945933-14945952 | None:intergenic | 35.0% | |
| ATGCTACACTATAGTATCAC+CGG | + | chr6.2:14946329-14946348 | None:intergenic | 35.0% | |
| CAAAGAAGCCAAAATTGCAT+TGG | - | chr6.2:14945995-14946014 | MS.gene074202:intron | 35.0% | |
| GATGTATATAGCAAGAACAG+TGG | - | chr6.2:14945871-14945890 | MS.gene074202:intron | 35.0% | |
| GTGAGCTTAGGATATTTACA+TGG | - | chr6.2:14946488-14946507 | MS.gene074202:intron | 35.0% | |
| TCGAAAAAGGAAGACTCAAA+GGG | - | chr6.2:14945958-14945977 | MS.gene074202:intron | 35.0% | |
| TCTTAGAACTAGAATTGCTG+CGG | + | chr6.2:14946708-14946727 | None:intergenic | 35.0% | |
| TTCGAAAAAGGAAGACTCAA+AGG | - | chr6.2:14945957-14945976 | MS.gene074202:intron | 35.0% | |
| ! | AAGTTGCCTTTTACACTCTT+CGG | - | chr6.2:14946053-14946072 | MS.gene074202:intron | 35.0% |
| !! | TAGTTCTAAGAAATAGAGCG+CGG | - | chr6.2:14946717-14946736 | MS.gene074202:intron | 35.0% |
| !! | TGTTTGTTGTGAACATTTGC+AGG | - | chr6.2:14947037-14947056 | MS.gene074202:CDS | 35.0% |
| AAAAAGGAAGACTCAAAGGG+TGG | - | chr6.2:14945961-14945980 | MS.gene074202:intron | 40.0% | |
| ACTATTTGACAACACTAGCC+AGG | + | chr6.2:14946302-14946321 | None:intergenic | 40.0% | |
| AGAAGCCAAAATTGCATTGG+GGG | - | chr6.2:14945998-14946017 | MS.gene074202:intron | 40.0% | |
| ATCACTGCAAACGGAACTTT+GGG | - | chr6.2:14946246-14946265 | MS.gene074202:intron | 40.0% | |
| CGCATATCGCAGAACAAAAA+TGG | + | chr6.2:14946360-14946379 | None:intergenic | 40.0% | |
| CTATTTGACAACACTAGCCA+GGG | + | chr6.2:14946301-14946320 | None:intergenic | 40.0% | |
| TACACTCTTCGGAAGGAATT+TGG | - | chr6.2:14946064-14946083 | MS.gene074202:intron | 40.0% | |
| TGATCCATAATCGACAACAC+TGG | - | chr6.2:14946447-14946466 | MS.gene074202:intron | 40.0% | |
| TGGCTAGTGTTGTCAAATAG+TGG | - | chr6.2:14946301-14946320 | MS.gene074202:intron | 40.0% | |
| ! | CTATACCCCCAATGCAATTT+TGG | + | chr6.2:14946006-14946025 | None:intergenic | 40.0% |
| ! | TTCCTTCCGAAGAGTGTAAA+AGG | + | chr6.2:14946062-14946081 | None:intergenic | 40.0% |
| AAATAGCGGCTGTAGCATAG+CGG | - | chr6.2:14946400-14946419 | MS.gene074202:intron | 45.0% | |
| AGTGAAATTTCGCGTTTCCC+TGG | - | chr6.2:14946281-14946300 | MS.gene074202:intron | 45.0% | |
| CCTAAGCTCACTCTACTAGT+GGG | + | chr6.2:14946479-14946498 | None:intergenic | 45.0% | |
| GATCACTGCAAACGGAACTT+TGG | - | chr6.2:14946245-14946264 | MS.gene074202:intron | 45.0% | |
| GTGTTGTCAAATAGTGGCTC+CGG | - | chr6.2:14946307-14946326 | MS.gene074202:intron | 45.0% | |
| TCACTGCAAACGGAACTTTG+GGG | - | chr6.2:14946247-14946266 | MS.gene074202:intron | 45.0% | |
| TCCTAAGCTCACTCTACTAG+TGG | + | chr6.2:14946480-14946499 | None:intergenic | 45.0% | |
| TGATCCGTGCAGCTAAAATC+AGG | + | chr6.2:14946765-14946784 | None:intergenic | 45.0% | |
| TGCAGATGACGCCAAAAAGA+AGG | - | chr6.2:14945837-14945856 | MS.gene074202:intron | 45.0% | |
| ! | AGGACCAGTGTTGTCGATTA+TGG | + | chr6.2:14946454-14946473 | None:intergenic | 45.0% |
| ! | ATTGCCTCGTGCCTTCTTTT+TGG | + | chr6.2:14945851-14945870 | None:intergenic | 45.0% |
| ! | TATTAGCGGTGTCAACGAGT+CGG | + | chr6.2:14946837-14946856 | None:intergenic | 45.0% |
| ! | TGCCTTTTACACTCTTCGGA+AGG | - | chr6.2:14946057-14946076 | MS.gene074202:intron | 45.0% |
| !!! | CGATCCTGATTTTAGCTGCA+CGG | - | chr6.2:14946758-14946777 | MS.gene074202:intron | 45.0% |
| AGCAAGAACAGTGGAGCTCA+AGG | - | chr6.2:14945880-14945899 | MS.gene074202:intron | 50.0% | |
| CATTTGCAGGCTTGACACCA+AGG | - | chr6.2:14947050-14947069 | MS.gene074202:CDS | 50.0% | |
| CCCACTAGTAGAGTGAGCTT+AGG | - | chr6.2:14946476-14946495 | MS.gene074202:intron | 50.0% | |
| GATAAGGCGTGTCCCAGTGT+CGG | + | chr6.2:14946911-14946930 | None:intergenic | 55.0% | |
| GCTTGACACCAAGGAGAGGT+GGG | - | chr6.2:14947059-14947078 | MS.gene074202:CDS | 55.0% | |
| TATGAAGCACGGACGCTCCT+CGG | + | chr6.2:14946933-14946952 | None:intergenic | 55.0% | |
| TTTCCTCTCCCACCTCTCCT+TGG | + | chr6.2:14947070-14947089 | None:intergenic | 55.0% | |
| ! | GACGCCAAAAAGAAGGCACG+AGG | - | chr6.2:14945844-14945863 | MS.gene074202:intron | 55.0% |
| ! | GCTCACTCTACTAGTGGGTC+AGG | + | chr6.2:14946474-14946493 | None:intergenic | 55.0% |
| ACACCAAGGAGAGGTGGGAG+AGG | - | chr6.2:14947064-14947083 | MS.gene074202:CDS | 60.0% | |
| ACTGGGACACGCCTTATCCG+AGG | - | chr6.2:14946913-14946932 | MS.gene074202:CDS | 60.0% | |
| CGGCTATCGTGTCCGACACT+GGG | - | chr6.2:14946896-14946915 | MS.gene074202:CDS | 60.0% | |
| GCAGGCTTGACACCAAGGAG+AGG | - | chr6.2:14947055-14947074 | MS.gene074202:CDS | 60.0% | |
| GGCTTGACACCAAGGAGAGG+TGG | - | chr6.2:14947058-14947077 | MS.gene074202:CDS | 60.0% | |
| TCGGCTATCGTGTCCGACAC+TGG | - | chr6.2:14946895-14946914 | MS.gene074202:CDS | 60.0% | |
| GCACGGACGCTCCTCGGATA+AGG | + | chr6.2:14946927-14946946 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 14945808 | 14947101 | 14945808 | ID=MS.gene074202 |
| chr6.2 | mRNA | 14945808 | 14947101 | 14945808 | ID=MS.gene074202.t1;Parent=MS.gene074202 |
| chr6.2 | exon | 14946890 | 14947101 | 14946890 | ID=MS.gene074202.t1.exon1;Parent=MS.gene074202.t1 |
| chr6.2 | CDS | 14946890 | 14947101 | 14946890 | ID=cds.MS.gene074202.t1;Parent=MS.gene074202.t1 |
| chr6.2 | exon | 14945808 | 14945850 | 14945808 | ID=MS.gene074202.t1.exon2;Parent=MS.gene074202.t1 |
| chr6.2 | CDS | 14945808 | 14945850 | 14945808 | ID=cds.MS.gene074202.t1;Parent=MS.gene074202.t1 |
| Gene Sequence |
| Protein sequence |
