Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49793.t1 | XP_013451570.1 | 95.2 | 63 | 3 | 0 | 398 | 460 | 355 | 417 | 4.50E-20 | 109.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49793.t1 | A0A072U911 | 95.2 | 63 | 3 | 0 | 398 | 460 | 355 | 417 | 3.3e-20 | 109.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene49793 | 0.831294 | 1.81E-55 | -1.69E-46 |
| MS.gene049159 | MS.gene49793 | 0.822957 | 1.79E-53 | -1.69E-46 |
| MS.gene04918 | MS.gene49793 | 0.848046 | 7.86E-60 | -1.69E-46 |
| MS.gene04920 | MS.gene49793 | 0.832713 | 8.08E-56 | -1.69E-46 |
| MS.gene049254 | MS.gene49793 | 0.859764 | 3.30E-63 | -1.69E-46 |
| MS.gene049283 | MS.gene49793 | 0.803677 | 3.11E-49 | -1.69E-46 |
| MS.gene049343 | MS.gene49793 | 0.843841 | 1.09E-58 | -1.69E-46 |
| MS.gene049368 | MS.gene49793 | 0.826343 | 2.86E-54 | -1.69E-46 |
| MS.gene049404 | MS.gene49793 | 0.8108 | 9.63E-51 | -1.69E-46 |
| MS.gene049452 | MS.gene49793 | 0.809961 | 1.46E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene49793 | 0.831985 | 1.22E-55 | -1.69E-46 |
| MS.gene049501 | MS.gene49793 | 0.82153 | 3.85E-53 | -1.69E-46 |
| MS.gene049711 | MS.gene49793 | 0.811035 | 8.56E-51 | -1.69E-46 |
| MS.gene049808 | MS.gene49793 | 0.806744 | 7.09E-50 | -1.69E-46 |
| MS.gene049873 | MS.gene49793 | 0.816569 | 5.17E-52 | -1.69E-46 |
| MS.gene049983 | MS.gene49793 | 0.852107 | 5.73E-61 | -1.69E-46 |
| MS.gene050026 | MS.gene49793 | 0.806542 | 7.82E-50 | -1.69E-46 |
| MS.gene050559 | MS.gene49793 | 0.819592 | 1.07E-52 | -1.69E-46 |
| MS.gene050561 | MS.gene49793 | 0.815551 | 8.73E-52 | -1.69E-46 |
| MS.gene050689 | MS.gene49793 | 0.811706 | 6.12E-51 | -1.69E-46 |
| MS.gene051003 | MS.gene49793 | 0.83007 | 3.61E-55 | -1.69E-46 |
| MS.gene051198 | MS.gene49793 | 0.815252 | 1.02E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene49793 | 0.820507 | 6.61E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene49793 | 0.82093 | 5.29E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene49793 | 0.832366 | 9.85E-56 | -1.69E-46 |
| MS.gene05134 | MS.gene49793 | 0.806176 | 9.34E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene49793 | 0.812354 | 4.42E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene49793 | 0.806892 | 6.60E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene49793 | 0.818436 | 1.96E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene49793 | 0.843971 | 1.01E-58 | -1.69E-46 |
| MS.gene051975 | MS.gene49793 | 0.801529 | 8.62E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene49793 | 0.816186 | 6.30E-52 | -1.69E-46 |
| MS.gene052516 | MS.gene49793 | 0.83601 | 1.20E-56 | -1.69E-46 |
| MS.gene052518 | MS.gene49793 | 0.825263 | 5.16E-54 | -1.69E-46 |
| MS.gene052519 | MS.gene49793 | 0.882643 | 8.77E-71 | -1.69E-46 |
| MS.gene052534 | MS.gene49793 | 0.804426 | 2.17E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene49793 | 0.839445 | 1.58E-57 | -1.69E-46 |
| MS.gene052584 | MS.gene49793 | 0.851945 | 6.38E-61 | -1.69E-46 |
| MS.gene052773 | MS.gene49793 | 0.825518 | 4.49E-54 | -1.69E-46 |
| MS.gene052863 | MS.gene49793 | 0.833726 | 4.52E-56 | -1.69E-46 |
| MS.gene053006 | MS.gene49793 | 0.852855 | 3.51E-61 | -1.69E-46 |
| MS.gene053008 | MS.gene49793 | 0.839389 | 1.63E-57 | -1.69E-46 |
| MS.gene053079 | MS.gene49793 | 0.81674 | 4.73E-52 | -1.69E-46 |
| MS.gene053122 | MS.gene49793 | 0.822966 | 1.79E-53 | -1.69E-46 |
| MS.gene053226 | MS.gene49793 | 0.857257 | 1.85E-62 | -1.69E-46 |
| MS.gene053272 | MS.gene49793 | -0.832555 | 8.84E-56 | -1.69E-46 |
| MS.gene05364 | MS.gene49793 | 0.841744 | 3.95E-58 | -1.69E-46 |
| MS.gene053931 | MS.gene49793 | 0.838268 | 3.18E-57 | -1.69E-46 |
| MS.gene054142 | MS.gene49793 | 0.81121 | 7.85E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene49793 | 0.812118 | 4.98E-51 | -1.69E-46 |
| MS.gene05458 | MS.gene49793 | 0.82962 | 4.65E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene49793 | 0.870639 | 1.25E-66 | -1.69E-46 |
| MS.gene054775 | MS.gene49793 | 0.833083 | 6.54E-56 | -1.69E-46 |
| MS.gene05517 | MS.gene49793 | 0.828044 | 1.12E-54 | -1.69E-46 |
| MS.gene055228 | MS.gene49793 | 0.808694 | 2.73E-50 | -1.69E-46 |
| MS.gene055464 | MS.gene49793 | 0.807289 | 5.44E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene49793 | 0.818134 | 2.30E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene49793 | 0.812382 | 4.36E-51 | -1.69E-46 |
| MS.gene055512 | MS.gene49793 | 0.802326 | 5.92E-49 | -1.69E-46 |
| MS.gene055513 | MS.gene49793 | 0.834351 | 3.15E-56 | -1.69E-46 |
| MS.gene055580 | MS.gene49793 | -0.820463 | 6.77E-53 | -1.69E-46 |
| MS.gene055586 | MS.gene49793 | 0.814284 | 1.67E-51 | -1.69E-46 |
| MS.gene055589 | MS.gene49793 | 0.80841 | 3.14E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene49793 | 0.803217 | 3.87E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene49793 | 0.819859 | 9.31E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene49793 | 0.84028 | 9.56E-58 | -1.69E-46 |
| MS.gene056043 | MS.gene49793 | -0.806418 | 8.31E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene49793 | 0.821922 | 3.12E-53 | -1.69E-46 |
| MS.gene056215 | MS.gene49793 | 0.818461 | 1.94E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene49793 | 0.82341 | 1.41E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene49793 | 0.821107 | 4.81E-53 | -1.69E-46 |
| MS.gene056595 | MS.gene49793 | 0.833957 | 3.96E-56 | -1.69E-46 |
| MS.gene056699 | MS.gene49793 | 0.828307 | 9.67E-55 | -1.69E-46 |
| MS.gene056776 | MS.gene49793 | 0.822473 | 2.32E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene49793 | 0.846035 | 2.80E-59 | -1.69E-46 |
| MS.gene05690 | MS.gene49793 | 0.857876 | 1.21E-62 | -1.69E-46 |
| MS.gene057034 | MS.gene49793 | 0.815885 | 7.35E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene49793 | 0.844481 | 7.36E-59 | -1.69E-46 |
| MS.gene057208 | MS.gene49793 | 0.859523 | 3.90E-63 | -1.69E-46 |
| MS.gene057444 | MS.gene49793 | 0.840726 | 7.31E-58 | -1.69E-46 |
| MS.gene057598 | MS.gene49793 | 0.806754 | 7.06E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene49793 | 0.820426 | 6.90E-53 | -1.69E-46 |
| MS.gene057694 | MS.gene49793 | 0.806632 | 7.49E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene49793 | 0.811801 | 5.84E-51 | -1.69E-46 |
| MS.gene057932 | MS.gene49793 | 0.830363 | 3.06E-55 | -1.69E-46 |
| MS.gene058008 | MS.gene49793 | 0.840012 | 1.12E-57 | -1.69E-46 |
| MS.gene058009 | MS.gene49793 | 0.859419 | 4.19E-63 | -1.69E-46 |
| MS.gene058010 | MS.gene49793 | 0.809565 | 1.78E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene49793 | 0.820487 | 6.68E-53 | -1.69E-46 |
| MS.gene058088 | MS.gene49793 | 0.812484 | 4.14E-51 | -1.69E-46 |
| MS.gene058279 | MS.gene49793 | 0.812832 | 3.48E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene49793 | 0.82824 | 1.00E-54 | -1.69E-46 |
| MS.gene058781 | MS.gene49793 | 0.855325 | 6.80E-62 | -1.69E-46 |
| MS.gene058783 | MS.gene49793 | 0.878454 | 2.77E-69 | -1.69E-46 |
| MS.gene058979 | MS.gene49793 | 0.842099 | 3.18E-58 | -1.69E-46 |
| MS.gene059146 | MS.gene49793 | 0.812455 | 4.20E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene49793 | 0.808896 | 2.47E-50 | -1.69E-46 |
| MS.gene059283 | MS.gene49793 | 0.800138 | 1.66E-48 | -1.69E-46 |
| MS.gene05952 | MS.gene49793 | 0.806853 | 6.72E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene49793 | 0.808556 | 2.92E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene49793 | 0.844116 | 9.22E-59 | -1.69E-46 |
| MS.gene059842 | MS.gene49793 | 0.856114 | 4.00E-62 | -1.69E-46 |
| MS.gene05985 | MS.gene49793 | -0.831927 | 1.26E-55 | -1.69E-46 |
| MS.gene059901 | MS.gene49793 | -0.819534 | 1.10E-52 | -1.69E-46 |
| MS.gene059935 | MS.gene49793 | 0.801044 | 1.08E-48 | -1.69E-46 |
| MS.gene060244 | MS.gene49793 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene49793 | 0.845596 | 3.68E-59 | -1.69E-46 |
| MS.gene060322 | MS.gene49793 | 0.828739 | 7.60E-55 | -1.69E-46 |
| MS.gene060365 | MS.gene49793 | 0.806023 | 1.01E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene49793 | 0.834153 | 3.53E-56 | -1.69E-46 |
| MS.gene060935 | MS.gene49793 | 0.861943 | 7.19E-64 | -1.69E-46 |
| MS.gene060937 | MS.gene49793 | 0.840516 | 8.29E-58 | -1.69E-46 |
| MS.gene061100 | MS.gene49793 | 0.838405 | 2.93E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49793.t1 | MTR_6g028130 | 92.386 | 197 | 15 | 0 | 1 | 197 | 1 | 197 | 9.72e-33 | 129 |
| MS.gene49793.t1 | MTR_6g028130 | 94.059 | 101 | 6 | 0 | 360 | 460 | 317 | 417 | 3.99e-26 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 78 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGTTGCTGAGGATAAAA+AGG | 0.295046 | 6.3:-20281878 | MS.gene49793:CDS |
| CTTCAGCTTGCTGACATCAT+TGG | 0.314579 | 6.3:+20280997 | None:intergenic |
| GGATGAAAAGAAGAAGAAAA+AGG | 0.339456 | 6.3:-20281812 | MS.gene49793:CDS |
| TGGCGAAGAGAATAAGAAAA+AGG | 0.375379 | 6.3:-20281362 | MS.gene49793:CDS |
| CTTCTTCTCTTCTCCATCAT+CGG | 0.378473 | 6.3:+20281570 | None:intergenic |
| GAAAAGAAAGACAAGGGAAA+GGG | 0.380268 | 6.3:-20281397 | MS.gene49793:CDS |
| GAAAAGAAAGACAAGGGAAA+GGG | 0.380268 | 6.3:-20281532 | MS.gene49793:CDS |
| GAAAGAGAAGAAAGATGATA+AGG | 0.398401 | 6.3:-20281176 | MS.gene49793:CDS |
| GAAGTTGTTTCGAGGGAGAT+AGG | 0.398534 | 6.3:-20281151 | MS.gene49793:CDS |
| GGGAAGAAGAGAAAGTTATC+TGG | 0.404078 | 6.3:-20281031 | MS.gene49793:CDS |
| TCAGATGATTCTAAAGATAT+CGG | 0.416847 | 6.3:-20282075 | MS.gene49793:CDS |
| CAGCTACTCAACCGCATCCT+TGG | 0.418843 | 6.3:+20280862 | None:intergenic |
| GAAAGAAAAGGTGAAAAGTA+AGG | 0.419739 | 6.3:-20281920 | MS.gene49793:CDS |
| ATCTTCTTCCGCTTTCTCCT+CGG | 0.426624 | 6.3:+20281117 | None:intergenic |
| TGAGAAAGAAGGTGATGTAA+AGG | 0.431997 | 6.3:-20282181 | MS.gene49793:CDS |
| CTTCTTCGCTTCTCCTTCAT+CGG | 0.436907 | 6.3:+20281276 | None:intergenic |
| AGAAACACTTCTTGAACAAA+AGG | 0.438069 | 6.3:-20280945 | MS.gene49793:CDS |
| GTTCTTCTCTTCTCCATCAT+CGG | 0.438481 | 6.3:+20281435 | None:intergenic |
| CAAAAGCTTGAAAAGATCAA+TGG | 0.445389 | 6.3:-20280974 | MS.gene49793:CDS |
| AGGAGAGGATAGTGAAAAGA+AGG | 0.464984 | 6.3:-20281740 | MS.gene49793:CDS |
| GAAGATGAGGAAATTGATGA+TGG | 0.465754 | 6.3:-20281100 | MS.gene49793:CDS |
| AGAAAAGAAAGACAAGGGAA+AGG | 0.469773 | 6.3:-20281398 | MS.gene49793:CDS |
| AGAAAAGAAAGACAAGGGAA+AGG | 0.469773 | 6.3:-20281533 | MS.gene49793:CDS |
| GAAAACAGAGAAGAAAGAAA+AGG | 0.469942 | 6.3:-20281932 | MS.gene49793:CDS |
| AAAAGCTTGAAAAGATCAAT+GGG | 0.470870 | 6.3:-20280973 | MS.gene49793:CDS |
| TGAAGGTGAAGACGGTAAGA+AGG | 0.475165 | 6.3:-20281845 | MS.gene49793:CDS |
| GAAGAGAAAGTTATCTGGAA+AGG | 0.479246 | 6.3:-20281026 | MS.gene49793:CDS |
| AAACAAGGGTGAGGAAGAAG+AGG | 0.485502 | 6.3:-20281230 | MS.gene49793:CDS |
| ACACGGTGACAAGGAAGAAA+AGG | 0.490673 | 6.3:-20282031 | MS.gene49793:CDS |
| GGGGAAAGAAGATGTAGGAG+AGG | 0.491438 | 6.3:-20281755 | MS.gene49793:CDS |
| AAGGAGAATAAAGACAAGGA+TGG | 0.499016 | 6.3:-20281901 | MS.gene49793:CDS |
| CGATGAAGGAGAAGCGAAGA+AGG | 0.499033 | 6.3:-20281275 | MS.gene49793:CDS |
| GTGGTGAAATCGACGGGAAA+GGG | 0.501431 | 6.3:-20281667 | MS.gene49793:CDS |
| TCTAAAGATATCGGTGAAGA+TGG | 0.508358 | 6.3:-20282066 | MS.gene49793:CDS |
| TGATAAAATCAAGCCGAAGA+AGG | 0.516483 | 6.3:-20281311 | MS.gene49793:CDS |
| CTTCAGTTCTACTTCTAACT+CGG | 0.517888 | 6.3:+20282149 | None:intergenic |
| CGATGATGGAGAAGAGAAGA+AGG | 0.519003 | 6.3:-20281569 | MS.gene49793:CDS |
| AGGTTATGTCGTCGTGGAGA+AGG | 0.529920 | 6.3:-20280885 | MS.gene49793:CDS |
| GGAAAGGGAGATGAGGATGA+TGG | 0.533215 | 6.3:-20281382 | MS.gene49793:CDS |
| ATAAGGATGAAGTTGTTTCG+AGG | 0.535585 | 6.3:-20281159 | MS.gene49793:CDS |
| AAAGAAGACAAGGACAAAGG+TGG | 0.536507 | 6.3:-20281052 | MS.gene49793:CDS |
| AGACAAGGGAAAGGGAGATG+AGG | 0.545147 | 6.3:-20281389 | MS.gene49793:CDS |
| CGTGGAGAAGGCCAAGGATG+CGG | 0.547057 | 6.3:-20280873 | MS.gene49793:CDS |
| TGGGAAGGAAGTGAAAGAGA+AGG | 0.550834 | 6.3:-20281080 | MS.gene49793:CDS |
| TGAAGAGATCGAAACAAAGA+AGG | 0.552163 | 6.3:-20281782 | MS.gene49793:CDS |
| TAAGGATGAAGTTGTTTCGA+GGG | 0.553952 | 6.3:-20281158 | MS.gene49793:CDS |
| TGTGGTGAAATCGACGGGAA+AGG | 0.558629 | 6.3:-20281668 | MS.gene49793:CDS |
| AAAGGTGATGAAGGTGAAGA+CGG | 0.561058 | 6.3:-20281853 | MS.gene49793:CDS |
| AAGAAGACAAGGACAAAGGT+GGG | 0.568761 | 6.3:-20281051 | MS.gene49793:CDS |
| CGAGTTAGAAGTAGAACTGA+AGG | 0.569653 | 6.3:-20282148 | MS.gene49793:CDS |
| TGAGGAAATTGATGATGGGA+AGG | 0.571455 | 6.3:-20281095 | MS.gene49793:CDS |
| AAGACTGAGGATGAGAAAGA+AGG | 0.573061 | 6.3:-20282192 | MS.gene49793:CDS |
| GAATAAAGACAAGGATGGAG+TGG | 0.576722 | 6.3:-20281896 | MS.gene49793:CDS |
| GGAGAAAGCGGAAGAAGATG+AGG | 0.582784 | 6.3:-20281113 | MS.gene49793:CDS |
| AACAAGGGTGAGGAAGAAGA+GGG | 0.584773 | 6.3:-20281229 | MS.gene49793:CDS |
| GGAAAGGGAGACGAGGATGA+CGG | 0.585914 | 6.3:-20281517 | MS.gene49793:CDS |
| GATGATGTGGTGAAATCGAC+GGG | 0.591968 | 6.3:-20281673 | MS.gene49793:CDS |
| ATCGAAACAAAGAAGGACAA+GGG | 0.592480 | 6.3:-20281775 | MS.gene49793:CDS |
| ATACGAGAAGCAGAAGATGA+AGG | 0.592634 | 6.3:-20280905 | MS.gene49793:CDS |
| AAGATGAGGAAATTGATGAT+GGG | 0.593304 | 6.3:-20281099 | MS.gene49793:CDS |
| GACAAGGGGAAAGAAGATGT+AGG | 0.593494 | 6.3:-20281760 | MS.gene49793:CDS |
| ATCGACGGGAAAGGGAGAAG+AGG | 0.608750 | 6.3:-20281659 | MS.gene49793:CDS |
| TCGAAACAAAGAAGGACAAG+GGG | 0.610740 | 6.3:-20281774 | MS.gene49793:CDS |
| ACAAAAGACCGAGGAGAAAG+CGG | 0.613724 | 6.3:-20281125 | MS.gene49793:CDS |
| GATCGAAACAAAGAAGGACA+AGG | 0.618475 | 6.3:-20281776 | MS.gene49793:CDS |
| CAAGGATGGAGTGGTTGCTG+AGG | 0.621637 | 6.3:-20281887 | MS.gene49793:CDS |
| TGAGAATAAACACGGTGACA+AGG | 0.628299 | 6.3:-20282040 | MS.gene49793:CDS |
| GGAAAGGGAGAAGAGGATGA+CGG | 0.633241 | 6.3:-20281652 | MS.gene49793:CDS |
| AGACAAGGGAAAGGGAGACG+AGG | 0.635113 | 6.3:-20281524 | MS.gene49793:CDS |
| TGTCGTCGTGGAGAAGGCCA+AGG | 0.642134 | 6.3:-20280879 | MS.gene49793:CDS |
| GTTGTTTCGAGGGAGATAGG+TGG | 0.649540 | 6.3:-20281148 | MS.gene49793:CDS |
| CTCTCTAAAGATCAAGACTG+AGG | 0.653524 | 6.3:-20282205 | MS.gene49793:CDS |
| TGATGATGTGGTGAAATCGA+CGG | 0.654535 | 6.3:-20281674 | MS.gene49793:CDS |
| AAGTAAGGAGAATAAAGACA+AGG | 0.656406 | 6.3:-20281905 | MS.gene49793:CDS |
| AAAGCACAAGGATGATGATG+TGG | 0.673676 | 6.3:-20281686 | MS.gene49793:CDS |
| AGATGAAGGTTATGTCGTCG+TGG | 0.684945 | 6.3:-20280891 | MS.gene49793:CDS |
| GATGGTAGTGAGAATAAACA+CGG | 0.724895 | 6.3:-20282048 | MS.gene49793:CDS |
| GATAGGTGGACAAAAGACCG+AGG | 0.748856 | 6.3:-20281134 | MS.gene49793:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGAAAAGAAAAACAA+GGG | - | chr6.3:20281846-20281865 | MS.gene49793:CDS | 15.0% |
| !! | AAAAAAGAAAAGAAAGACAA+GGG | - | chr6.3:20281552-20281571 | MS.gene49793:CDS | 20.0% |
| !! | GAAAAAAGAAAAGAAAAACA+AGG | - | chr6.3:20281845-20281864 | MS.gene49793:CDS | 20.0% |
| ! | AAAAGCTTGAAAAGATCAAT+GGG | - | chr6.3:20282117-20282136 | MS.gene49793:CDS | 25.0% |
| ! | GAAAAAAGAAAAGAAAGACA+AGG | - | chr6.3:20281551-20281570 | MS.gene49793:CDS | 25.0% |
| !! | TCAGATGATTCTAAAGATAT+CGG | - | chr6.3:20281015-20281034 | MS.gene49793:CDS | 25.0% |
| AAAAAAGAAGACAAGGACAA+AGG | - | chr6.3:20282035-20282054 | MS.gene49793:CDS | 30.0% | |
| AAAAAGCGAAAAGAAAGAGA+AGG | - | chr6.3:20281374-20281393 | MS.gene49793:CDS | 30.0% | |
| AAAGAAAAAGACGACAAAAG+TGG | - | chr6.3:20281102-20281121 | MS.gene49793:CDS | 30.0% | |
| AAAGAAAAAGAGAAGGATAG+TGG | - | chr6.3:20281492-20281511 | MS.gene49793:CDS | 30.0% | |
| AAGTAAGGAGAATAAAGACA+AGG | - | chr6.3:20281185-20281204 | MS.gene49793:CDS | 30.0% | |
| AGAAAAGGACAAGAAAAAGA+AGG | - | chr6.3:20281074-20281093 | MS.gene49793:CDS | 30.0% | |
| AGAAACACTTCTTGAACAAA+AGG | - | chr6.3:20282145-20282164 | MS.gene49793:CDS | 30.0% | |
| AGAGAGTAAGAAAAAGAAGA+AGG | - | chr6.3:20281464-20281483 | MS.gene49793:CDS | 30.0% | |
| CAAAAGCTTGAAAAGATCAA+TGG | - | chr6.3:20282116-20282135 | MS.gene49793:CDS | 30.0% | |
| GAAAAAAAAAGAGAAGGACA+AGG | - | chr6.3:20281755-20281774 | MS.gene49793:CDS | 30.0% | |
| GAAAACAGAGAAGAAAGAAA+AGG | - | chr6.3:20281158-20281177 | MS.gene49793:CDS | 30.0% | |
| GAAAGAAAAGGTGAAAAGTA+AGG | - | chr6.3:20281170-20281189 | MS.gene49793:CDS | 30.0% | |
| GAAAGAGAAGAAAGATGATA+AGG | - | chr6.3:20281914-20281933 | MS.gene49793:CDS | 30.0% | |
| GGATAAGAAAGAAAAAGAGA+AGG | - | chr6.3:20281485-20281504 | MS.gene49793:CDS | 30.0% | |
| GGATGAAAAGAAGAAGAAAA+AGG | - | chr6.3:20281278-20281297 | MS.gene49793:CDS | 30.0% | |
| GGATGAGAAAAAAAAAGAGA+AGG | - | chr6.3:20281749-20281768 | MS.gene49793:CDS | 30.0% | |
| ! | AAGATGAGGAAATTGATGAT+GGG | - | chr6.3:20281991-20282010 | MS.gene49793:CDS | 30.0% |
| ! | AGAGAAAAAGAAAAAGCACA+AGG | - | chr6.3:20280981-20281000 | MS.gene49793:CDS | 30.0% |
| !!! | GTTTTTTCACTTTCCTTCTT+CGG | + | chr6.3:20281795-20281814 | None:intergenic | 30.0% |
| AAAAAGGGTAAAGGTGATGA+AGG | - | chr6.3:20281228-20281247 | MS.gene49793:CDS | 35.0% | |
| AAAGAAAAAGAGAAGGACAG+TGG | - | chr6.3:20281627-20281646 | MS.gene49793:CDS | 35.0% | |
| AAGGAGAATAAAGACAAGGA+TGG | - | chr6.3:20281189-20281208 | MS.gene49793:CDS | 35.0% | |
| AGAAAAGAAAAACAAGGGTG+AGG | - | chr6.3:20281851-20281870 | MS.gene49793:CDS | 35.0% | |
| AGAAAAGAAAGACAAGGGAA+AGG | - | chr6.3:20281557-20281576 | MS.gene49793:CDS | 35.0% | |
| ATCGAAACAAAGAAGGACAA+GGG | - | chr6.3:20281315-20281334 | MS.gene49793:CDS | 35.0% | |
| CTTCAGTTCTACTTCTAACT+CGG | + | chr6.3:20280944-20280963 | None:intergenic | 35.0% | |
| GAAAAGAAAGACAAGGGAAA+GGG | - | chr6.3:20281558-20281577 | MS.gene49793:CDS | 35.0% | |
| GAAAGTGAAAAAACCGATGA+AGG | - | chr6.3:20281801-20281820 | MS.gene49793:CDS | 35.0% | |
| GAAGAGAAAGTTATCTGGAA+AGG | - | chr6.3:20282064-20282083 | MS.gene49793:CDS | 35.0% | |
| GAAGATGAGGAAATTGATGA+TGG | - | chr6.3:20281990-20282009 | MS.gene49793:CDS | 35.0% | |
| GAAGGAGAAAAAAGAAGACA+AGG | - | chr6.3:20282028-20282047 | MS.gene49793:CDS | 35.0% | |
| TCTAAAGATATCGGTGAAGA+TGG | - | chr6.3:20281024-20281043 | MS.gene49793:CDS | 35.0% | |
| TGAAGAGATCGAAACAAAGA+AGG | - | chr6.3:20281308-20281327 | MS.gene49793:CDS | 35.0% | |
| TGAGAAAGAAGGTGATGTAA+AGG | - | chr6.3:20280909-20280928 | MS.gene49793:CDS | 35.0% | |
| TGATAAAATCAAGCCGAAGA+AGG | - | chr6.3:20281779-20281798 | MS.gene49793:CDS | 35.0% | |
| TGGCGAAGAGAATAAGAAAA+AGG | - | chr6.3:20281728-20281747 | MS.gene49793:CDS | 35.0% | |
| ! | ATAAGGATGAAGTTGTTTCG+AGG | - | chr6.3:20281931-20281950 | MS.gene49793:CDS | 35.0% |
| ! | GAAGGATAAGAAAAAGCACA+AGG | - | chr6.3:20281392-20281411 | MS.gene49793:CDS | 35.0% |
| ! | GATGGTAGTGAGAATAAACA+CGG | - | chr6.3:20281042-20281061 | MS.gene49793:CDS | 35.0% |
| ! | TAAGGATGAAGTTGTTTCGA+GGG | - | chr6.3:20281932-20281951 | MS.gene49793:CDS | 35.0% |
| AAAGAAGACAAGGACAAAGG+TGG | - | chr6.3:20282038-20282057 | MS.gene49793:CDS | 40.0% | |
| AAAGGTGATGAAGGTGAAGA+CGG | - | chr6.3:20281237-20281256 | MS.gene49793:CDS | 40.0% | |
| AAGAAGACAAGGACAAAGGT+GGG | - | chr6.3:20282039-20282058 | MS.gene49793:CDS | 40.0% | |
| AAGACTGAGGATGAGAAAGA+AGG | - | chr6.3:20280898-20280917 | MS.gene49793:CDS | 40.0% | |
| AGGAGAGGATAGTGAAAAGA+AGG | - | chr6.3:20281350-20281369 | MS.gene49793:CDS | 40.0% | |
| AGTGGTTGCTGAGGATAAAA+AGG | - | chr6.3:20281212-20281231 | MS.gene49793:CDS | 40.0% | |
| ATACGAGAAGCAGAAGATGA+AGG | - | chr6.3:20282185-20282204 | MS.gene49793:CDS | 40.0% | |
| CGAGTTAGAAGTAGAACTGA+AGG | - | chr6.3:20280942-20280961 | MS.gene49793:CDS | 40.0% | |
| CGGTAAGAAGGAGAAAAAGA+AGG | - | chr6.3:20281257-20281276 | MS.gene49793:CDS | 40.0% | |
| CTTCTTCTCTTCTCCATCAT+CGG | + | chr6.3:20281523-20281542 | None:intergenic | 40.0% | |
| GAATAAAGACAAGGATGGAG+TGG | - | chr6.3:20281194-20281213 | MS.gene49793:CDS | 40.0% | |
| GATCGAAACAAAGAAGGACA+AGG | - | chr6.3:20281314-20281333 | MS.gene49793:CDS | 40.0% | |
| GCTGAGGATAAAAAGGGTAA+AGG | - | chr6.3:20281219-20281238 | MS.gene49793:CDS | 40.0% | |
| GGGAAGAAGAGAAAGTTATC+TGG | - | chr6.3:20282059-20282078 | MS.gene49793:CDS | 40.0% | |
| GTGGTTGCTGAGGATAAAAA+GGG | - | chr6.3:20281213-20281232 | MS.gene49793:CDS | 40.0% | |
| GTTCTTCTCTTCTCCATCAT+CGG | + | chr6.3:20281658-20281677 | None:intergenic | 40.0% | |
| TCGAAACAAAGAAGGACAAG+GGG | - | chr6.3:20281316-20281335 | MS.gene49793:CDS | 40.0% | |
| TGAGAATAAACACGGTGACA+AGG | - | chr6.3:20281050-20281069 | MS.gene49793:CDS | 40.0% | |
| ! | AAAGCACAAGGATGATGATG+TGG | - | chr6.3:20281404-20281423 | MS.gene49793:CDS | 40.0% |
| ! | CTCTCTAAAGATCAAGACTG+AGG | - | chr6.3:20280885-20280904 | MS.gene49793:CDS | 40.0% |
| ! | TGATGATGTGGTGAAATCGA+CGG | - | chr6.3:20281416-20281435 | MS.gene49793:CDS | 40.0% |
| !! | GATAGTGGAAAAACCGATGA+TGG | - | chr6.3:20281507-20281526 | MS.gene49793:CDS | 40.0% |
| !! | TGAGGAAATTGATGATGGGA+AGG | - | chr6.3:20281995-20282014 | MS.gene49793:CDS | 40.0% |
| AAACAAGGGTGAGGAAGAAG+AGG | - | chr6.3:20281860-20281879 | MS.gene49793:CDS | 45.0% | |
| AACAAGGGTGAGGAAGAAGA+GGG | - | chr6.3:20281861-20281880 | MS.gene49793:CDS | 45.0% | |
| ACAAAAGACCGAGGAGAAAG+CGG | - | chr6.3:20281965-20281984 | MS.gene49793:CDS | 45.0% | |
| ACACGGTGACAAGGAAGAAA+AGG | - | chr6.3:20281059-20281078 | MS.gene49793:CDS | 45.0% | |
| AGATGAAGGTTATGTCGTCG+TGG | - | chr6.3:20282199-20282218 | MS.gene49793:CDS | 45.0% | |
| ATCTTCTTCCGCTTTCTCCT+CGG | + | chr6.3:20281976-20281995 | None:intergenic | 45.0% | |
| CTTCTTCGCTTCTCCTTCAT+CGG | + | chr6.3:20281817-20281836 | None:intergenic | 45.0% | |
| GACAAGGGGAAAGAAGATGT+AGG | - | chr6.3:20281330-20281349 | MS.gene49793:CDS | 45.0% | |
| GACAGTGGAAAAACCGATGA+TGG | - | chr6.3:20281642-20281661 | MS.gene49793:CDS | 45.0% | |
| GATGATGTGGTGAAATCGAC+GGG | - | chr6.3:20281417-20281436 | MS.gene49793:CDS | 45.0% | |
| TGAAGGTGAAGACGGTAAGA+AGG | - | chr6.3:20281245-20281264 | MS.gene49793:CDS | 45.0% | |
| TGGGAAGGAAGTGAAAGAGA+AGG | - | chr6.3:20282010-20282029 | MS.gene49793:CDS | 45.0% | |
| ! | GAAGTTGTTTCGAGGGAGAT+AGG | - | chr6.3:20281939-20281958 | MS.gene49793:CDS | 45.0% |
| !! | CGATGATGGAGAAGAGAAGA+AGG | - | chr6.3:20281521-20281540 | MS.gene49793:CDS | 45.0% |
| !! | CTTCAGCTTGCTGACATCAT+TGG | + | chr6.3:20282096-20282115 | None:intergenic | 45.0% |
| AGACAAGGGAAAGGGAGATG+AGG | - | chr6.3:20281701-20281720 | MS.gene49793:CDS | 50.0% | |
| AGGTTATGTCGTCGTGGAGA+AGG | - | chr6.3:20282205-20282224 | MS.gene49793:CDS | 50.0% | |
| CGATGAAGGAGAAGCGAAGA+AGG | - | chr6.3:20281815-20281834 | MS.gene49793:CDS | 50.0% | |
| GATAGGTGGACAAAAGACCG+AGG | - | chr6.3:20281956-20281975 | MS.gene49793:CDS | 50.0% | |
| GGAAAGGGAGAAGAGGATGA+CGG | - | chr6.3:20281438-20281457 | MS.gene49793:CDS | 50.0% | |
| GGAAAGGGAGATGAGGATGA+TGG | - | chr6.3:20281708-20281727 | MS.gene49793:CDS | 50.0% | |
| GGAGAAAGCGGAAGAAGATG+AGG | - | chr6.3:20281977-20281996 | MS.gene49793:CDS | 50.0% | |
| GGGGAAAGAAGATGTAGGAG+AGG | - | chr6.3:20281335-20281354 | MS.gene49793:CDS | 50.0% | |
| GTGGTGAAATCGACGGGAAA+GGG | - | chr6.3:20281423-20281442 | MS.gene49793:CDS | 50.0% | |
| TGTGGTGAAATCGACGGGAA+AGG | - | chr6.3:20281422-20281441 | MS.gene49793:CDS | 50.0% | |
| ! | GTTGTTTCGAGGGAGATAGG+TGG | - | chr6.3:20281942-20281961 | MS.gene49793:CDS | 50.0% |
| AGACAAGGGAAAGGGAGACG+AGG | - | chr6.3:20281566-20281585 | MS.gene49793:CDS | 55.0% | |
| ATCGACGGGAAAGGGAGAAG+AGG | - | chr6.3:20281431-20281450 | MS.gene49793:CDS | 55.0% | |
| CAAGGATGGAGTGGTTGCTG+AGG | - | chr6.3:20281203-20281222 | MS.gene49793:CDS | 55.0% | |
| GGAAAGGGAGACGAGGATGA+CGG | - | chr6.3:20281573-20281592 | MS.gene49793:CDS | 55.0% | |
| CGTGGAGAAGGCCAAGGATG+CGG | - | chr6.3:20282217-20282236 | MS.gene49793:CDS | 60.0% | |
| TGTCGTCGTGGAGAAGGCCA+AGG | - | chr6.3:20282211-20282230 | MS.gene49793:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 20280865 | 20282247 | 20280865 | ID=MS.gene49793 |
| chr6.3 | mRNA | 20280865 | 20282247 | 20280865 | ID=MS.gene49793.t1;Parent=MS.gene49793 |
| chr6.3 | exon | 20280865 | 20282247 | 20280865 | ID=MS.gene49793.t1.exon1;Parent=MS.gene49793.t1 |
| chr6.3 | CDS | 20280865 | 20282247 | 20280865 | ID=cds.MS.gene49793.t1;Parent=MS.gene49793.t1 |
| Gene Sequence |
| Protein sequence |
