Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29920.t1 | GAU17568.1 | 64.1 | 334 | 105 | 5 | 1 | 330 | 404 | 726 | 4.60E-107 | 397.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29920.t1 | Q9FPT1 | 46.2 | 130 | 67 | 3 | 10 | 138 | 56 | 183 | 6.7e-22 | 106.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29920.t1 | A0A392MFG0 | 65.3 | 334 | 101 | 5 | 1 | 330 | 4 | 326 | 5.0e-111 | 410.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene29920 | 0.825002 | 5.94E-54 | -1.69E-46 |
| MS.gene049350 | MS.gene29920 | 0.820442 | 6.85E-53 | -1.69E-46 |
| MS.gene049404 | MS.gene29920 | 0.812762 | 3.60E-51 | -1.69E-46 |
| MS.gene049807 | MS.gene29920 | 0.828112 | 1.08E-54 | -1.69E-46 |
| MS.gene049808 | MS.gene29920 | 0.807597 | 4.68E-50 | -1.69E-46 |
| MS.gene050237 | MS.gene29920 | 0.83215 | 1.11E-55 | -1.69E-46 |
| MS.gene050273 | MS.gene29920 | 0.804329 | 2.28E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene29920 | 0.814331 | 1.63E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene29920 | 0.840068 | 1.09E-57 | -1.69E-46 |
| MS.gene050561 | MS.gene29920 | 0.832851 | 7.47E-56 | -1.69E-46 |
| MS.gene050562 | MS.gene29920 | 0.82488 | 6.35E-54 | -1.69E-46 |
| MS.gene050799 | MS.gene29920 | 0.804987 | 1.66E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene29920 | 0.84062 | 7.79E-58 | -1.69E-46 |
| MS.gene051699 | MS.gene29920 | 0.810786 | 9.69E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene29920 | 0.809683 | 1.68E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene29920 | 0.819494 | 1.13E-52 | -1.69E-46 |
| MS.gene051744 | MS.gene29920 | 0.800827 | 1.20E-48 | -1.69E-46 |
| MS.gene052239 | MS.gene29920 | -0.841948 | 3.49E-58 | -1.69E-46 |
| MS.gene052447 | MS.gene29920 | 0.831265 | 1.84E-55 | -1.69E-46 |
| MS.gene052516 | MS.gene29920 | 0.817381 | 3.40E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene29920 | 0.83245 | 9.39E-56 | -1.69E-46 |
| MS.gene052582 | MS.gene29920 | 0.811731 | 6.05E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene29920 | 0.815366 | 9.59E-52 | -1.69E-46 |
| MS.gene052793 | MS.gene29920 | 0.805495 | 1.30E-49 | -1.69E-46 |
| MS.gene053082 | MS.gene29920 | 0.825153 | 5.47E-54 | -1.69E-46 |
| MS.gene053396 | MS.gene29920 | 0.800668 | 1.29E-48 | -1.69E-46 |
| MS.gene053599 | MS.gene29920 | 0.822617 | 2.15E-53 | -1.69E-46 |
| MS.gene05364 | MS.gene29920 | 0.805567 | 1.25E-49 | -1.69E-46 |
| MS.gene053678 | MS.gene29920 | 0.802635 | 5.11E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene29920 | 0.808067 | 3.71E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene29920 | 0.815886 | 7.35E-52 | -1.69E-46 |
| MS.gene055464 | MS.gene29920 | 0.805211 | 1.49E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene29920 | 0.806161 | 9.41E-50 | -1.69E-46 |
| MS.gene055580 | MS.gene29920 | -0.803067 | 4.16E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene29920 | 0.815413 | 9.36E-52 | -1.69E-46 |
| MS.gene055840 | MS.gene29920 | 0.807653 | 4.55E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene29920 | 0.811534 | 6.67E-51 | -1.69E-46 |
| MS.gene056174 | MS.gene29920 | 0.80308 | 4.13E-49 | -1.69E-46 |
| MS.gene056272 | MS.gene29920 | 0.832437 | 9.46E-56 | -1.69E-46 |
| MS.gene056308 | MS.gene29920 | 0.823754 | 1.17E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene29920 | 0.819687 | 1.02E-52 | -1.69E-46 |
| MS.gene056595 | MS.gene29920 | 0.80842 | 3.12E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene29920 | 0.815992 | 6.96E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene29920 | 0.813847 | 2.08E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene29920 | 0.805064 | 1.60E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene29920 | 0.820629 | 6.20E-53 | -1.69E-46 |
| MS.gene057694 | MS.gene29920 | 0.800014 | 1.76E-48 | -1.69E-46 |
| MS.gene057932 | MS.gene29920 | 0.804413 | 2.19E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene29920 | 0.833469 | 5.24E-56 | -1.69E-46 |
| MS.gene058009 | MS.gene29920 | 0.835353 | 1.76E-56 | -1.69E-46 |
| MS.gene058294 | MS.gene29920 | 0.800743 | 1.25E-48 | -1.69E-46 |
| MS.gene058330 | MS.gene29920 | 0.81199 | 5.31E-51 | -1.69E-46 |
| MS.gene059015 | MS.gene29920 | 0.835138 | 2.00E-56 | -1.69E-46 |
| MS.gene059625 | MS.gene29920 | 0.805064 | 1.60E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene29920 | -0.802747 | 4.85E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene29920 | 0.818318 | 2.09E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene29920 | MS.gene057066 | PPI |
| MS.gene29920 | MS.gene038327 | PPI |
| MS.gene29920 | MS.gene057069 | PPI |
| MS.gene29920 | MS.gene017099 | PPI |
| MS.gene29920 | MS.gene030037 | PPI |
| MS.gene29920 | MS.gene057090 | PPI |
| MS.gene29920 | MS.gene038300 | PPI |
| MS.gene29920 | MS.gene057070 | PPI |
| MS.gene29920 | MS.gene030045 | PPI |
| MS.gene29920 | MS.gene038342 | PPI |
| MS.gene29920 | MS.gene017100 | PPI |
| MS.gene29920 | MS.gene030026 | PPI |
| MS.gene29920 | MS.gene038330 | PPI |
| MS.gene29920 | MS.gene017102 | PPI |
| MS.gene29920 | MS.gene038326 | PPI |
| MS.gene29920 | MS.gene017083 | PPI |
| MS.gene038336 | MS.gene29920 | PPI |
| MS.gene29920 | MS.gene038324 | PPI |
| MS.gene29920 | MS.gene038328 | PPI |
| MS.gene29920 | MS.gene030029 | PPI |
| MS.gene29920 | MS.gene030042 | PPI |
| MS.gene29920 | MS.gene017101 | PPI |
| MS.gene29920 | MS.gene057065 | PPI |
| MS.gene29920 | MS.gene038323 | PPI |
| MS.gene29920 | MS.gene038333 | PPI |
| MS.gene29920 | MS.gene038336 | PPI |
| MS.gene29920 | MS.gene030034 | PPI |
| MS.gene29920 | MS.gene017115 | PPI |
| MS.gene29920 | MS.gene030033 | PPI |
| MS.gene038323 | MS.gene29920 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29920.t1 | MTR_7g107200 | 45.867 | 375 | 157 | 6 | 1 | 329 | 1 | 375 | 4.60e-108 | 320 |
| MS.gene29920.t1 | MTR_1g042160 | 49.701 | 334 | 136 | 8 | 1 | 328 | 1 | 308 | 2.54e-100 | 298 |
| MS.gene29920.t1 | MTR_7g107160 | 53.687 | 339 | 118 | 12 | 9 | 330 | 129 | 445 | 1.45e-99 | 301 |
| MS.gene29920.t1 | MTR_7g107160 | 49.612 | 129 | 45 | 3 | 66 | 174 | 1 | 129 | 7.43e-31 | 121 |
| MS.gene29920.t1 | MTR_8g011730 | 37.079 | 356 | 174 | 11 | 9 | 329 | 13 | 353 | 5.16e-63 | 204 |
| MS.gene29920.t1 | MTR_7g107170 | 54.037 | 161 | 66 | 1 | 168 | 328 | 111 | 263 | 2.14e-49 | 166 |
| MS.gene29920.t1 | MTR_7g107170 | 73.563 | 87 | 23 | 0 | 233 | 319 | 1 | 87 | 8.71e-33 | 122 |
| MS.gene29920.t1 | MTR_2g034780 | 36.747 | 166 | 102 | 1 | 158 | 323 | 99 | 261 | 4.68e-29 | 113 |
| MS.gene29920.t1 | MTR_3g079840 | 40.845 | 142 | 78 | 4 | 1 | 138 | 44 | 183 | 5.03e-26 | 109 |
| MS.gene29920.t1 | MTR_3g079840 | 40.845 | 142 | 78 | 4 | 1 | 138 | 45 | 184 | 5.22e-26 | 109 |
| MS.gene29920.t1 | MTR_5g086700 | 39.552 | 134 | 78 | 3 | 4 | 136 | 55 | 186 | 9.44e-26 | 108 |
| MS.gene29920.t1 | MTR_5g086700 | 39.552 | 134 | 78 | 3 | 4 | 136 | 55 | 186 | 1.04e-25 | 108 |
| MS.gene29920.t1 | MTR_1g041240 | 42.308 | 130 | 71 | 3 | 10 | 138 | 59 | 185 | 1.14e-24 | 105 |
| MS.gene29920.t1 | MTR_1g041240 | 42.308 | 130 | 71 | 3 | 10 | 138 | 58 | 184 | 1.15e-24 | 105 |
| MS.gene29920.t1 | MTR_1g041240 | 42.308 | 130 | 71 | 3 | 10 | 138 | 57 | 183 | 1.20e-24 | 105 |
| MS.gene29920.t1 | MTR_5g091450 | 38.095 | 126 | 76 | 2 | 10 | 134 | 33 | 157 | 2.39e-23 | 101 |
| MS.gene29920.t1 | MTR_5g091180 | 35.570 | 149 | 89 | 4 | 1 | 145 | 34 | 179 | 5.90e-21 | 94.4 |
| MS.gene29920.t1 | MTR_5g091550 | 34.646 | 127 | 80 | 3 | 9 | 134 | 18 | 142 | 1.00e-15 | 78.2 |
| MS.gene29920.t1 | MTR_5g091550 | 34.646 | 127 | 80 | 3 | 9 | 134 | 18 | 142 | 1.18e-15 | 77.8 |
| MS.gene29920.t1 | MTR_3g106700 | 34.752 | 141 | 82 | 3 | 10 | 140 | 406 | 546 | 9.43e-14 | 72.8 |
| MS.gene29920.t1 | MTR_1g073800 | 32.824 | 131 | 83 | 5 | 10 | 138 | 49 | 176 | 4.22e-12 | 67.0 |
| MS.gene29920.t1 | MTR_1g044450 | 33.588 | 131 | 80 | 5 | 10 | 138 | 65 | 190 | 1.51e-11 | 65.9 |
| MS.gene29920.t1 | MTR_4g123750 | 25.652 | 230 | 137 | 7 | 5 | 222 | 14 | 221 | 6.02e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29920.t1 | AT5G06600 | 44.366 | 142 | 73 | 4 | 1 | 138 | 44 | 183 | 9.98e-27 | 111 |
| MS.gene29920.t1 | AT5G06600 | 44.366 | 142 | 73 | 4 | 1 | 138 | 43 | 182 | 1.05e-26 | 111 |
| MS.gene29920.t1 | AT5G06600 | 44.366 | 142 | 73 | 4 | 1 | 138 | 44 | 183 | 1.17e-26 | 111 |
| MS.gene29920.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 55 | 182 | 2.69e-25 | 107 |
| MS.gene29920.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 55 | 182 | 2.69e-25 | 107 |
| MS.gene29920.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 54 | 181 | 2.75e-25 | 107 |
| MS.gene29920.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 54 | 181 | 2.75e-25 | 107 |
| MS.gene29920.t1 | AT3G58340 | 25.517 | 290 | 168 | 9 | 5 | 268 | 3 | 270 | 2.81e-16 | 78.6 |
| MS.gene29920.t1 | AT3G58340 | 24.667 | 300 | 168 | 9 | 5 | 268 | 3 | 280 | 2.50e-15 | 76.3 |
| MS.gene29920.t1 | AT3G58220 | 25.822 | 213 | 140 | 6 | 11 | 218 | 13 | 212 | 9.84e-15 | 74.3 |
| MS.gene29920.t1 | AT3G58370 | 28.378 | 222 | 134 | 9 | 1 | 218 | 1 | 201 | 5.90e-14 | 71.6 |
| MS.gene29920.t1 | AT3G58220 | 23.469 | 294 | 174 | 7 | 11 | 259 | 13 | 300 | 7.33e-14 | 72.0 |
| MS.gene29920.t1 | AT3G58360 | 27.083 | 192 | 119 | 6 | 9 | 199 | 7 | 178 | 1.77e-13 | 70.5 |
| MS.gene29920.t1 | AT3G44790 | 25.197 | 254 | 175 | 7 | 9 | 258 | 4 | 246 | 2.96e-13 | 70.1 |
| MS.gene29920.t1 | AT3G58240 | 24.768 | 323 | 206 | 10 | 8 | 319 | 6 | 302 | 3.49e-13 | 69.7 |
| MS.gene29920.t1 | AT3G58220 | 27.072 | 181 | 130 | 2 | 11 | 190 | 13 | 192 | 7.10e-13 | 69.7 |
| MS.gene29920.t1 | AT3G58220 | 27.072 | 181 | 130 | 2 | 11 | 190 | 13 | 192 | 1.35e-12 | 68.2 |
| MS.gene29920.t1 | AT3G58220 | 30.469 | 128 | 85 | 3 | 11 | 135 | 13 | 139 | 2.04e-12 | 66.6 |
| MS.gene29920.t1 | AT3G27040 | 28.467 | 137 | 96 | 2 | 7 | 142 | 6 | 141 | 2.88e-12 | 66.6 |
| MS.gene29920.t1 | AT2G25320 | 32.278 | 158 | 96 | 4 | 10 | 157 | 405 | 561 | 4.85e-12 | 67.8 |
| MS.gene29920.t1 | AT2G25320 | 32.278 | 158 | 96 | 4 | 10 | 157 | 404 | 560 | 4.90e-12 | 67.8 |
| MS.gene29920.t1 | AT2G25320 | 32.278 | 158 | 96 | 4 | 10 | 157 | 405 | 561 | 1.04e-11 | 66.6 |
| MS.gene29920.t1 | AT2G25330 | 30.729 | 192 | 122 | 5 | 10 | 190 | 359 | 550 | 1.16e-11 | 66.2 |
| MS.gene29920.t1 | AT3G58210 | 32.824 | 131 | 85 | 3 | 8 | 136 | 6 | 135 | 1.83e-11 | 64.7 |
Find 62 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGCAAGTAAGGATTATT+TGG | 0.155785 | 8.1:-49675667 | MS.gene29920:CDS |
| GTACACTGCTAATATGTTTC+AGG | 0.178138 | 8.1:-49676715 | MS.gene29920:CDS |
| CCAAAGTCTCGACCACATTT+TGG | 0.217526 | 8.1:+49675463 | None:intergenic |
| CAAGTCAAATTGAAATTAAA+TGG | 0.219404 | 8.1:+49675213 | None:intergenic |
| TTCACAATAAACCCTCTTTC+AGG | 0.229550 | 8.1:+49675609 | None:intergenic |
| TTTGGAAAAGAACAGTTATT+TGG | 0.252160 | 8.1:-49675166 | MS.gene29920:CDS |
| CTCTCACCTTTCTTGTCTTA+AGG | 0.269117 | 8.1:+49675289 | None:intergenic |
| CGTTGGCCTTCTTCTAGTTC+TGG | 0.287736 | 8.1:+49675486 | None:intergenic |
| GAATGGGCGTTCACCGCTTT+GGG | 0.323269 | 8.1:-49675327 | MS.gene29920:CDS |
| GGAATGGGCGTTCACCGCTT+TGG | 0.324343 | 8.1:-49675328 | MS.gene29920:CDS |
| AGTAAGGATTATTTGGGTTT+CGG | 0.339740 | 8.1:-49675660 | MS.gene29920:CDS |
| TTCCTCTAGTAATGGAATAA+AGG | 0.345498 | 8.1:+49675410 | None:intergenic |
| GTGAAGGATGCTTGTATTGT+TGG | 0.355564 | 8.1:-49675591 | MS.gene29920:CDS |
| GCCTTGTGTTCAATCTGCTT+TGG | 0.362659 | 8.1:-49675184 | MS.gene29920:CDS |
| GGATGATTTGTCTGTATCAA+TGG | 0.368177 | 8.1:-49676782 | MS.gene29920:CDS |
| TACTCTGAGCCTTTCATCCT+TGG | 0.374548 | 8.1:-49676898 | MS.gene29920:CDS |
| TCTCTCACTTGAATAAGATT+AGG | 0.386650 | 8.1:-49676927 | MS.gene29920:CDS |
| CCAAAATGTGGTCGAGACTT+TGG | 0.387611 | 8.1:-49675463 | MS.gene29920:CDS |
| AACTCGCAGGTTTAGGGAAT+GGG | 0.389237 | 8.1:-49675343 | MS.gene29920:CDS |
| AATGCAAGTAAGGATTATTT+GGG | 0.389997 | 8.1:-49675666 | MS.gene29920:CDS |
| TGATGCAGCAAGAGACTTGT+TGG | 0.401616 | 8.1:-49675043 | MS.gene29920:CDS |
| GATGATTTGTCTGTATCAAT+GGG | 0.407748 | 8.1:-49676781 | MS.gene29920:CDS |
| CTTTGAGTTCAATGCAAGTA+AGG | 0.409663 | 8.1:-49675676 | MS.gene29920:CDS |
| CCTTCTTCTAGTTCTGGCTC+TGG | 0.429903 | 8.1:+49675492 | None:intergenic |
| GGTCGAGACTTTGGATTTCA+AGG | 0.447344 | 8.1:-49675454 | MS.gene29920:CDS |
| CAGTTATTTGGAGAAATTGA+AGG | 0.448720 | 8.1:-49675154 | MS.gene29920:CDS |
| GTTGAAGCAGTTAAGTTAGC+TGG | 0.452845 | 8.1:+49675519 | None:intergenic |
| GTTAGAATTGGAAAGGCTAA+TGG | 0.457549 | 8.1:-49675094 | MS.gene29920:CDS |
| CATTTAATTTCAATTTGACT+TGG | 0.466420 | 8.1:-49675212 | MS.gene29920:CDS |
| GGTAGCTCTAGAGTTAGAAT+TGG | 0.468868 | 8.1:-49675106 | MS.gene29920:CDS |
| ATGGATTTGGATGCTGAACT+AGG | 0.469976 | 8.1:-49675000 | MS.gene29920:CDS |
| TCTGAGCCTTTCATCCTTGG+CGG | 0.492833 | 8.1:-49676895 | MS.gene29920:CDS |
| GAGCTTCTTGATCCTGAAAG+AGG | 0.499293 | 8.1:-49675621 | MS.gene29920:CDS |
| TCATCCTTGGCGGCTATCCA+TGG | 0.501161 | 8.1:-49676885 | MS.gene29920:intron |
| AAACTCGCAGGTTTAGGGAA+TGG | 0.505546 | 8.1:-49675344 | MS.gene29920:CDS |
| TTATTTCCTTAAGACAAGAA+AGG | 0.506188 | 8.1:-49675295 | MS.gene29920:CDS |
| GTCGAGACTTTGGATTTCAA+GGG | 0.521430 | 8.1:-49675453 | MS.gene29920:CDS |
| TTTGGAGAAATTGAAGGAGG+CGG | 0.535745 | 8.1:-49675148 | MS.gene29920:CDS |
| AGCTTCTTGATCCTGAAAGA+GGG | 0.537917 | 8.1:-49675620 | MS.gene29920:CDS |
| GCCAGCAAGACACTTACAAA+AGG | 0.544024 | 8.1:-49676642 | MS.gene29920:intron |
| GAACATACTTCCTCTAGTAA+TGG | 0.544728 | 8.1:+49675402 | None:intergenic |
| TGAAAGAGGGTTTATTGTGA+AGG | 0.555016 | 8.1:-49675607 | MS.gene29920:CDS |
| TCCAAAGCAGATTGAACACA+AGG | 0.565327 | 8.1:+49675183 | None:intergenic |
| GGATAGCCGCCAAGGATGAA+AGG | 0.566403 | 8.1:+49676889 | None:intergenic |
| TCCTGACACAAAATAAGCGA+AGG | 0.566723 | 8.1:+49675372 | None:intergenic |
| CCAGAGCCAGAACTAGAAGA+AGG | 0.572474 | 8.1:-49675492 | MS.gene29920:CDS |
| CGAGCGATGAGAAGTTCACA+TGG | 0.574026 | 8.1:-49676963 | MS.gene29920:CDS |
| ACTTTGGATTTCAAGGGTGT+AGG | 0.575922 | 8.1:-49675447 | MS.gene29920:CDS |
| GGCCTTTATTCCATTACTAG+AGG | 0.578841 | 8.1:-49675412 | MS.gene29920:CDS |
| TACACTGCTAATATGTTTCA+GGG | 0.579049 | 8.1:-49676714 | MS.gene29920:CDS |
| CTGCTAATATGTTTCAGGGA+TGG | 0.579409 | 8.1:-49676710 | MS.gene29920:CDS |
| CTCTAGAGTTAGAATTGGAA+AGG | 0.585395 | 8.1:-49675101 | MS.gene29920:CDS |
| CATACCATGGATAGCCGCCA+AGG | 0.606316 | 8.1:+49676881 | None:intergenic |
| TGCAGCAAGAGACTTGTTGG+AGG | 0.618326 | 8.1:-49675040 | MS.gene29920:CDS |
| AGAAGGCCAACGCCAAAATG+TGG | 0.623253 | 8.1:-49675475 | MS.gene29920:CDS |
| TTATTTGGAGAAATTGAAGG+AGG | 0.644664 | 8.1:-49675151 | MS.gene29920:CDS |
| CCTGACACAAAATAAGCGAA+GGG | 0.648287 | 8.1:+49675373 | None:intergenic |
| GGGTGTAGGACAAATAGAGA+AGG | 0.651657 | 8.1:-49675433 | MS.gene29920:CDS |
| CTAACTTAACTGCTTCAACA+AGG | 0.670044 | 8.1:-49675515 | MS.gene29920:CDS |
| TGATTTGTCTGTATCAATGG+GGG | 0.682465 | 8.1:-49676779 | MS.gene29920:CDS |
| ATGATTTGTCTGTATCAATG+GGG | 0.692803 | 8.1:-49676780 | MS.gene29920:CDS |
| ATAAAGAACTCTGCCCAAAG+CGG | 0.731605 | 8.1:+49675314 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAGGAAAAATAAGAATT+TGG | - | chr8.1:49676111-49676130 | MS.gene29920:intron | 15.0% |
| !! | TAAGAATCTTCAAAAAATTT+GGG | - | chr8.1:49676716-49676735 | MS.gene29920:CDS | 15.0% |
| !! | TATTTGGTTAAAATAAGTTT+AGG | + | chr8.1:49675530-49675549 | None:intergenic | 15.0% |
| !!! | TATAACTTTTTTTGGTTATT+TGG | + | chr8.1:49675546-49675565 | None:intergenic | 15.0% |
| !! | AATACAATAATGAGCAAAAA+TGG | + | chr8.1:49675769-49675788 | None:intergenic | 20.0% |
| !! | ACATTAGAAGTTTAAAAAGT+CGG | + | chr8.1:49675887-49675906 | None:intergenic | 20.0% |
| !! | CAAGTCAAATTGAAATTAAA+TGG | + | chr8.1:49676753-49676772 | None:intergenic | 20.0% |
| !! | GTAAGAATCTTCAAAAAATT+TGG | - | chr8.1:49676715-49676734 | MS.gene29920:CDS | 20.0% |
| !!! | CATTTAATTTCAATTTGACT+TGG | - | chr8.1:49676751-49676770 | MS.gene29920:CDS | 20.0% |
| !!! | CTAAGTTATTTTAGATTAGT+CGG | - | chr8.1:49675395-49675414 | MS.gene29920:CDS | 20.0% |
| ! | AATGCAAGTAAGGATTATTT+GGG | - | chr8.1:49676297-49676316 | MS.gene29920:intron | 25.0% |
| ! | TGTTAGTAATTTCATAGAGT+TGG | - | chr8.1:49676205-49676224 | MS.gene29920:intron | 25.0% |
| ! | TTATTTCCTTAAGACAAGAA+AGG | - | chr8.1:49676668-49676687 | MS.gene29920:CDS | 25.0% |
| ! | TTCATTCAAATATCATACCA+TGG | + | chr8.1:49675098-49675117 | None:intergenic | 25.0% |
| ! | TTCCAACTCTTTCTAATATT+GGG | + | chr8.1:49675605-49675624 | None:intergenic | 25.0% |
| ! | TTGAATATTAGAGTACAACT+AGG | - | chr8.1:49675372-49675391 | MS.gene29920:CDS | 25.0% |
| ! | TTTCCAACTCTTTCTAATAT+TGG | + | chr8.1:49675606-49675625 | None:intergenic | 25.0% |
| ! | TTTGGAAAAGAACAGTTATT+TGG | - | chr8.1:49676797-49676816 | MS.gene29920:intron | 25.0% |
| !!! | CTTTTTATTTTTCAGTCTCA+AGG | - | chr8.1:49676035-49676054 | MS.gene29920:intron | 25.0% |
| CAATAGTTTCGTATCATATG+AGG | + | chr8.1:49675132-49675151 | None:intergenic | 30.0% | |
| CAATGCAAGTAAGGATTATT+TGG | - | chr8.1:49676296-49676315 | MS.gene29920:intron | 30.0% | |
| CAGTTATTTGGAGAAATTGA+AGG | - | chr8.1:49676809-49676828 | MS.gene29920:intron | 30.0% | |
| GAATCTTCAAAAAATTTGGG+AGG | - | chr8.1:49676719-49676738 | MS.gene29920:CDS | 30.0% | |
| GTATTTGTTCTATGTTGCAA+AGG | + | chr8.1:49675994-49676013 | None:intergenic | 30.0% | |
| TAAACAAACAGCAAAAGAGA+CGG | + | chr8.1:49676158-49676177 | None:intergenic | 30.0% | |
| TACACTGCTAATATGTTTCA+GGG | - | chr8.1:49675249-49675268 | MS.gene29920:CDS | 30.0% | |
| TCTCTCACTTGAATAAGATT+AGG | - | chr8.1:49675036-49675055 | MS.gene29920:CDS | 30.0% | |
| TTATTTGGAGAAATTGAAGG+AGG | - | chr8.1:49676812-49676831 | MS.gene29920:intron | 30.0% | |
| TTCCCAATATTAGAAAGAGT+TGG | - | chr8.1:49675600-49675619 | MS.gene29920:CDS | 30.0% | |
| TTGAACTCAAAGTTAGATTC+TGG | + | chr8.1:49676279-49676298 | None:intergenic | 30.0% | |
| ! | AAAAAGTCGGAATTTAGAGT+TGG | + | chr8.1:49675874-49675893 | None:intergenic | 30.0% |
| ! | AGTAAGGATTATTTGGGTTT+CGG | - | chr8.1:49676303-49676322 | MS.gene29920:intron | 30.0% |
| ! | AGTTAGATTCTGGAAAATTC+AGG | + | chr8.1:49676269-49676288 | None:intergenic | 30.0% |
| ! | ATGATTTGTCTGTATCAATG+GGG | - | chr8.1:49675183-49675202 | MS.gene29920:CDS | 30.0% |
| ! | GATGATTTGTCTGTATCAAT+GGG | - | chr8.1:49675182-49675201 | MS.gene29920:CDS | 30.0% |
| ! | TTCCTCTAGTAATGGAATAA+AGG | + | chr8.1:49676556-49676575 | None:intergenic | 30.0% |
| !! | AGATTTTGAAGAGATGGATT+TGG | - | chr8.1:49676950-49676969 | MS.gene29920:CDS | 30.0% |
| !! | TCAAGAAGTGTCAATTTAGT+TGG | + | chr8.1:49676244-49676263 | None:intergenic | 30.0% |
| !!! | TTGTGTTTTGACTTTGCTTT+AGG | - | chr8.1:49675160-49675179 | MS.gene29920:CDS | 30.0% |
| AGAAAAAAACTCGCAGGTTT+AGG | - | chr8.1:49676613-49676632 | MS.gene29920:intron | 35.0% | |
| AGGTCAAAAATCAAGACTCA+GGG | + | chr8.1:49675494-49675513 | None:intergenic | 35.0% | |
| CTAACTTAACTGCTTCAACA+AGG | - | chr8.1:49676448-49676467 | MS.gene29920:intron | 35.0% | |
| GAAAAAAACTCGCAGGTTTA+GGG | - | chr8.1:49676614-49676633 | MS.gene29920:intron | 35.0% | |
| GAACATACTTCCTCTAGTAA+TGG | + | chr8.1:49676564-49676583 | None:intergenic | 35.0% | |
| GGAAAAACTAAGGGATAATG+TGG | - | chr8.1:49676836-49676855 | MS.gene29920:intron | 35.0% | |
| GTACACTGCTAATATGTTTC+AGG | - | chr8.1:49675248-49675267 | MS.gene29920:CDS | 35.0% | |
| GTTAGAATTGGAAAGGCTAA+TGG | - | chr8.1:49676869-49676888 | MS.gene29920:intron | 35.0% | |
| TAGGTCAAAAATCAAGACTC+AGG | + | chr8.1:49675495-49675514 | None:intergenic | 35.0% | |
| TGAAAGAGGGTTTATTGTGA+AGG | - | chr8.1:49676356-49676375 | MS.gene29920:intron | 35.0% | |
| TGCGCTCAACAAAATCATAA+AGG | - | chr8.1:49676095-49676114 | MS.gene29920:intron | 35.0% | |
| TGTATTGTGCGTGAAATTTG+TGG | - | chr8.1:49675783-49675802 | MS.gene29920:intron | 35.0% | |
| TTCACAATAAACCCTCTTTC+AGG | + | chr8.1:49676357-49676376 | None:intergenic | 35.0% | |
| ! | ATTTTGTTGAGCGCAAAACA+TGG | + | chr8.1:49676090-49676109 | None:intergenic | 35.0% |
| ! | CTTTGAGTTCAATGCAAGTA+AGG | - | chr8.1:49676287-49676306 | MS.gene29920:intron | 35.0% |
| ! | GGATGATTTGTCTGTATCAA+TGG | - | chr8.1:49675181-49675200 | MS.gene29920:CDS | 35.0% |
| ! | TGATTTGTCTGTATCAATGG+GGG | - | chr8.1:49675184-49675203 | MS.gene29920:CDS | 35.0% |
| !! | CTCTAGAGTTAGAATTGGAA+AGG | - | chr8.1:49676862-49676881 | MS.gene29920:intron | 35.0% |
| !! | TAACTATACCTCTGAAGCAT+GGG | - | chr8.1:49675673-49675692 | MS.gene29920:CDS | 35.0% |
| !! | TTAACTATACCTCTGAAGCA+TGG | - | chr8.1:49675672-49675691 | MS.gene29920:CDS | 35.0% |
| AAACAGCAAAAGAGACGGAT+TGG | + | chr8.1:49676153-49676172 | None:intergenic | 40.0% | |
| AAACGGTGAAGTACATGTAC+CGG | - | chr8.1:49675701-49675720 | MS.gene29920:intron | 40.0% | |
| AGCTTCTTGATCCTGAAAGA+GGG | - | chr8.1:49676343-49676362 | MS.gene29920:intron | 40.0% | |
| ATAAAGAACTCTGCCCAAAG+CGG | + | chr8.1:49676652-49676671 | None:intergenic | 40.0% | |
| ATGAGTCACAGTAAGATTGC+AGG | - | chr8.1:49675430-49675449 | MS.gene29920:CDS | 40.0% | |
| ATGGATTTGGATGCTGAACT+AGG | - | chr8.1:49676963-49676982 | MS.gene29920:CDS | 40.0% | |
| CCTGACACAAAATAAGCGAA+GGG | + | chr8.1:49676593-49676612 | None:intergenic | 40.0% | |
| CTCTCACCTTTCTTGTCTTA+AGG | + | chr8.1:49676677-49676696 | None:intergenic | 40.0% | |
| CTGCTAATATGTTTCAGGGA+TGG | - | chr8.1:49675253-49675272 | MS.gene29920:CDS | 40.0% | |
| GGCCTTTATTCCATTACTAG+AGG | - | chr8.1:49676551-49676570 | MS.gene29920:intron | 40.0% | |
| GTCGAGACTTTGGATTTCAA+GGG | - | chr8.1:49676510-49676529 | MS.gene29920:intron | 40.0% | |
| GTGAAGGATGCTTGTATTGT+TGG | - | chr8.1:49676372-49676391 | MS.gene29920:intron | 40.0% | |
| GTTGAAGCAGTTAAGTTAGC+TGG | + | chr8.1:49676447-49676466 | None:intergenic | 40.0% | |
| TCCAAAGCAGATTGAACACA+AGG | + | chr8.1:49676783-49676802 | None:intergenic | 40.0% | |
| TCCTGACACAAAATAAGCGA+AGG | + | chr8.1:49676594-49676613 | None:intergenic | 40.0% | |
| TTTGGAGAAATTGAAGGAGG+CGG | - | chr8.1:49676815-49676834 | MS.gene29920:intron | 40.0% | |
| ! | ACCTTTTGTAAGTGTCTTGC+TGG | + | chr8.1:49675325-49675344 | None:intergenic | 40.0% |
| ! | ACTTTGGATTTCAAGGGTGT+AGG | - | chr8.1:49676516-49676535 | MS.gene29920:intron | 40.0% |
| ! | CTGAAGCATGGGTTGTAAAA+CGG | - | chr8.1:49675684-49675703 | MS.gene29920:CDS | 40.0% |
| ! | GTTTAGGTTCAGAGTTCACT+AGG | + | chr8.1:49675514-49675533 | None:intergenic | 40.0% |
| ! | TTTTACAACCCATGCTTCAG+AGG | + | chr8.1:49675684-49675703 | None:intergenic | 40.0% |
| !! | GGCAGAAGATTTTGAAGAGA+TGG | - | chr8.1:49676944-49676963 | MS.gene29920:CDS | 40.0% |
| !! | GGTAGCTCTAGAGTTAGAAT+TGG | - | chr8.1:49676857-49676876 | MS.gene29920:intron | 40.0% |
| AAACTCGCAGGTTTAGGGAA+TGG | - | chr8.1:49676619-49676638 | MS.gene29920:intron | 45.0% | |
| AACGGTGAAGTACATGTACC+GGG | - | chr8.1:49675702-49675721 | MS.gene29920:intron | 45.0% | |
| AACTCGCAGGTTTAGGGAAT+GGG | - | chr8.1:49676620-49676639 | MS.gene29920:intron | 45.0% | |
| CCAAAATGTGGTCGAGACTT+TGG | - | chr8.1:49676500-49676519 | MS.gene29920:intron | 45.0% | |
| GAAGGAGGCGGAAAAACTAA+GGG | - | chr8.1:49676827-49676846 | MS.gene29920:intron | 45.0% | |
| GAGCTTCTTGATCCTGAAAG+AGG | - | chr8.1:49676342-49676361 | MS.gene29920:intron | 45.0% | |
| GCCAGCAAGACACTTACAAA+AGG | - | chr8.1:49675321-49675340 | MS.gene29920:CDS | 45.0% | |
| GCCTTGTGTTCAATCTGCTT+TGG | - | chr8.1:49676779-49676798 | MS.gene29920:CDS | 45.0% | |
| GGGTGTAGGACAAATAGAGA+AGG | - | chr8.1:49676530-49676549 | MS.gene29920:intron | 45.0% | |
| GGTCGAGACTTTGGATTTCA+AGG | - | chr8.1:49676509-49676528 | MS.gene29920:intron | 45.0% | |
| GTCAGGAGAAAAAAACTCGC+AGG | - | chr8.1:49676607-49676626 | MS.gene29920:intron | 45.0% | |
| TACTCTGAGCCTTTCATCCT+TGG | - | chr8.1:49675065-49675084 | MS.gene29920:CDS | 45.0% | |
| TGAAGGAGGCGGAAAAACTA+AGG | - | chr8.1:49676826-49676845 | MS.gene29920:intron | 45.0% | |
| TGATGCAGCAAGAGACTTGT+TGG | - | chr8.1:49676920-49676939 | MS.gene29920:CDS | 45.0% | |
| ! | CCAAAGTCTCGACCACATTT+TGG | + | chr8.1:49676503-49676522 | None:intergenic | 45.0% |
| !!! | CCCTTCGCTTATTTTGTGTC+AGG | - | chr8.1:49676590-49676609 | MS.gene29920:intron | 45.0% |
| !!! | TTTAATTTTATAACTTTTTT+TGG | + | chr8.1:49675554-49675573 | None:intergenic | 5.0% |
| AGAAGGCCAACGCCAAAATG+TGG | - | chr8.1:49676488-49676507 | MS.gene29920:intron | 50.0% | |
| CCAGAGCCAGAACTAGAAGA+AGG | - | chr8.1:49676471-49676490 | MS.gene29920:intron | 50.0% | |
| CGAGCGATGAGAAGTTCACA+TGG | - | chr8.1:49675000-49675019 | MS.gene29920:CDS | 50.0% | |
| TCTGAGCCTTTCATCCTTGG+CGG | - | chr8.1:49675068-49675087 | MS.gene29920:CDS | 50.0% | |
| TGTCTGTATCAATGGGGGAC+AGG | - | chr8.1:49675189-49675208 | MS.gene29920:CDS | 50.0% | |
| !! | CCTTCTTCTAGTTCTGGCTC+TGG | + | chr8.1:49676474-49676493 | None:intergenic | 50.0% |
| !! | CGTTGGCCTTCTTCTAGTTC+TGG | + | chr8.1:49676480-49676499 | None:intergenic | 50.0% |
| !! | TCTCGACCACATTTTGGCGT+TGG | + | chr8.1:49676497-49676516 | None:intergenic | 50.0% |
| !! | TGCAGCAAGAGACTTGTTGG+AGG | - | chr8.1:49676923-49676942 | MS.gene29920:CDS | 50.0% |
| CATACCATGGATAGCCGCCA+AGG | + | chr8.1:49675085-49675104 | None:intergenic | 55.0% | |
| GAATGGGCGTTCACCGCTTT+GGG | - | chr8.1:49676636-49676655 | MS.gene29920:intron | 55.0% | |
| GGATAGCCGCCAAGGATGAA+AGG | + | chr8.1:49675077-49675096 | None:intergenic | 55.0% | |
| TACCGTACGCGTACCCAGTA+CGG | + | chr8.1:49675735-49675754 | None:intergenic | 55.0% | |
| TCATCCTTGGCGGCTATCCA+TGG | - | chr8.1:49675078-49675097 | MS.gene29920:CDS | 55.0% | |
| ACCCAGTACGGGTGTGTACC+CGG | + | chr8.1:49675723-49675742 | None:intergenic | 60.0% | |
| ACCCGTACTGGGTACGCGTA+CGG | - | chr8.1:49675730-49675749 | MS.gene29920:intron | 60.0% | |
| ACCGGGTACACACCCGTACT+GGG | - | chr8.1:49675719-49675738 | MS.gene29920:intron | 60.0% | |
| ACCGTACGCGTACCCAGTAC+GGG | + | chr8.1:49675734-49675753 | None:intergenic | 60.0% | |
| GGAATGGGCGTTCACCGCTT+TGG | - | chr8.1:49676635-49676654 | MS.gene29920:intron | 60.0% | |
| GGGTACGCGTACGGTACTTG+TGG | - | chr8.1:49675739-49675758 | MS.gene29920:intron | 60.0% | |
| TACCGGGTACACACCCGTAC+TGG | - | chr8.1:49675718-49675737 | MS.gene29920:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 49674984 | 49677001 | 49674984 | ID=MS.gene29920 |
| chr8.1 | mRNA | 49674984 | 49677001 | 49674984 | ID=MS.gene29920.t1;Parent=MS.gene29920 |
| chr8.1 | exon | 49676886 | 49677001 | 49676886 | ID=MS.gene29920.t1.exon1;Parent=MS.gene29920.t1 |
| chr8.1 | CDS | 49676886 | 49677001 | 49676886 | ID=cds.MS.gene29920.t1;Parent=MS.gene29920.t1 |
| chr8.1 | exon | 49676643 | 49676803 | 49676643 | ID=MS.gene29920.t1.exon2;Parent=MS.gene29920.t1 |
| chr8.1 | CDS | 49676643 | 49676803 | 49676643 | ID=cds.MS.gene29920.t1;Parent=MS.gene29920.t1 |
| chr8.1 | exon | 49674984 | 49675705 | 49674984 | ID=MS.gene29920.t1.exon3;Parent=MS.gene29920.t1 |
| chr8.1 | CDS | 49674984 | 49675705 | 49674984 | ID=cds.MS.gene29920.t1;Parent=MS.gene29920.t1 |
| Gene Sequence |
| Protein sequence |
