Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49946.t1 | XP_013453058.1 | 93.6 | 375 | 19 | 3 | 1 | 375 | 1 | 370 | 5.90E-167 | 597 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49946.t1 | Q9LJG8 | 39.8 | 98 | 53 | 2 | 133 | 227 | 84 | 178 | 4.9e-13 | 77.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49946.t1 | A0A072UC93 | 93.6 | 375 | 19 | 3 | 1 | 375 | 1 | 370 | 4.2e-167 | 597.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene49946.t1 | TF | Trihelix |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene49946 | 0.802259 | 6.11E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene49946 | 0.841951 | 3.48E-58 | -1.69E-46 |
| MS.gene04920 | MS.gene49946 | 0.800992 | 1.11E-48 | -1.69E-46 |
| MS.gene049254 | MS.gene49946 | 0.860672 | 1.75E-63 | -1.69E-46 |
| MS.gene049343 | MS.gene49946 | 0.816528 | 5.28E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene49946 | 0.835693 | 1.45E-56 | -1.69E-46 |
| MS.gene049435 | MS.gene49946 | 0.811028 | 8.59E-51 | -1.69E-46 |
| MS.gene049452 | MS.gene49946 | 0.849719 | 2.70E-60 | -1.69E-46 |
| MS.gene049821 | MS.gene49946 | 0.800493 | 1.40E-48 | -1.69E-46 |
| MS.gene049983 | MS.gene49946 | 0.826859 | 2.15E-54 | -1.69E-46 |
| MS.gene050026 | MS.gene49946 | 0.814102 | 1.83E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene49946 | 0.801597 | 8.35E-49 | -1.69E-46 |
| MS.gene050605 | MS.gene49946 | 0.803953 | 2.73E-49 | -1.69E-46 |
| MS.gene050750 | MS.gene49946 | 0.819886 | 9.18E-53 | -1.69E-46 |
| MS.gene050759 | MS.gene49946 | 0.821393 | 4.13E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene49946 | 0.815876 | 7.38E-52 | -1.69E-46 |
| MS.gene050962 | MS.gene49946 | 0.809215 | 2.11E-50 | -1.69E-46 |
| MS.gene051066 | MS.gene49946 | 0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene051181 | MS.gene49946 | 0.817141 | 3.85E-52 | -1.69E-46 |
| MS.gene051198 | MS.gene49946 | 0.862562 | 4.64E-64 | -1.69E-46 |
| MS.gene051279 | MS.gene49946 | 0.807905 | 4.02E-50 | -1.69E-46 |
| MS.gene051368 | MS.gene49946 | 0.803764 | 2.98E-49 | -1.69E-46 |
| MS.gene051597 | MS.gene49946 | 0.821855 | 3.23E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene49946 | 0.827137 | 1.85E-54 | -1.69E-46 |
| MS.gene052012 | MS.gene49946 | 0.804736 | 1.87E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene49946 | 0.800865 | 1.18E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene49946 | 0.805465 | 1.32E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene49946 | 0.804877 | 1.75E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene49946 | 0.854841 | 9.41E-62 | -1.69E-46 |
| MS.gene052582 | MS.gene49946 | 0.853797 | 1.88E-61 | -1.69E-46 |
| MS.gene052584 | MS.gene49946 | 0.853178 | 2.84E-61 | -1.69E-46 |
| MS.gene052773 | MS.gene49946 | 0.816421 | 5.58E-52 | -1.69E-46 |
| MS.gene052863 | MS.gene49946 | 0.812083 | 5.07E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene49946 | 0.83481 | 2.42E-56 | -1.69E-46 |
| MS.gene053008 | MS.gene49946 | 0.829433 | 5.16E-55 | -1.69E-46 |
| MS.gene053079 | MS.gene49946 | 0.839078 | 1.96E-57 | -1.69E-46 |
| MS.gene05308 | MS.gene49946 | 0.819301 | 1.25E-52 | -1.69E-46 |
| MS.gene053226 | MS.gene49946 | 0.839633 | 1.41E-57 | -1.69E-46 |
| MS.gene053321 | MS.gene49946 | 0.814283 | 1.67E-51 | -1.69E-46 |
| MS.gene05364 | MS.gene49946 | 0.808322 | 3.28E-50 | -1.69E-46 |
| MS.gene053931 | MS.gene49946 | 0.815425 | 9.31E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene49946 | 0.820031 | 8.50E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene49946 | 0.805256 | 1.46E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene49946 | 0.804932 | 1.70E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene49946 | -0.815601 | 8.50E-52 | -1.69E-46 |
| MS.gene054405 | MS.gene49946 | 0.806242 | 9.05E-50 | -1.69E-46 |
| MS.gene054479 | MS.gene49946 | 0.811707 | 6.12E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene49946 | 0.873696 | 1.20E-67 | -1.69E-46 |
| MS.gene054657 | MS.gene49946 | 0.842762 | 2.12E-58 | -1.69E-46 |
| MS.gene054775 | MS.gene49946 | 0.848042 | 7.89E-60 | -1.69E-46 |
| MS.gene054991 | MS.gene49946 | 0.803483 | 3.41E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene49946 | 0.808131 | 3.60E-50 | -1.69E-46 |
| MS.gene055222 | MS.gene49946 | 0.813503 | 2.48E-51 | -1.69E-46 |
| MS.gene055228 | MS.gene49946 | 0.834587 | 2.75E-56 | -1.69E-46 |
| MS.gene055513 | MS.gene49946 | 0.820931 | 5.28E-53 | -1.69E-46 |
| MS.gene055586 | MS.gene49946 | 0.820787 | 5.70E-53 | -1.69E-46 |
| MS.gene055589 | MS.gene49946 | 0.810125 | 1.35E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene49946 | 0.809532 | 1.81E-50 | -1.69E-46 |
| MS.gene055724 | MS.gene49946 | 0.802358 | 5.83E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene49946 | 0.835599 | 1.53E-56 | -1.69E-46 |
| MS.gene055841 | MS.gene49946 | 0.852691 | 3.91E-61 | -1.69E-46 |
| MS.gene055926 | MS.gene49946 | 0.822396 | 2.42E-53 | -1.69E-46 |
| MS.gene056050 | MS.gene49946 | 0.80313 | 4.04E-49 | -1.69E-46 |
| MS.gene056162 | MS.gene49946 | 0.802373 | 5.79E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene49946 | 0.826068 | 3.32E-54 | -1.69E-46 |
| MS.gene056215 | MS.gene49946 | 0.808009 | 3.82E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene49946 | 0.835449 | 1.67E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene49946 | 0.820721 | 5.91E-53 | -1.69E-46 |
| MS.gene056595 | MS.gene49946 | 0.812347 | 4.44E-51 | -1.69E-46 |
| MS.gene056776 | MS.gene49946 | 0.862898 | 3.66E-64 | -1.69E-46 |
| MS.gene056779 | MS.gene49946 | 0.808022 | 3.80E-50 | -1.69E-46 |
| MS.gene05686 | MS.gene49946 | 0.80037 | 1.49E-48 | -1.69E-46 |
| MS.gene05690 | MS.gene49946 | 0.82511 | 5.61E-54 | -1.69E-46 |
| MS.gene057034 | MS.gene49946 | 0.820644 | 6.15E-53 | -1.69E-46 |
| MS.gene057060 | MS.gene49946 | 0.808817 | 2.57E-50 | -1.69E-46 |
| MS.gene057208 | MS.gene49946 | 0.87087 | 1.05E-66 | -1.69E-46 |
| MS.gene057314 | MS.gene49946 | 0.817131 | 3.87E-52 | -1.69E-46 |
| MS.gene057444 | MS.gene49946 | 0.8202 | 7.78E-53 | -1.69E-46 |
| MS.gene057598 | MS.gene49946 | 0.824024 | 1.01E-53 | -1.69E-46 |
| MS.gene057693 | MS.gene49946 | 0.819163 | 1.34E-52 | -1.69E-46 |
| MS.gene057932 | MS.gene49946 | 0.810025 | 1.41E-50 | -1.69E-46 |
| MS.gene058008 | MS.gene49946 | 0.812962 | 3.26E-51 | -1.69E-46 |
| MS.gene058009 | MS.gene49946 | 0.83163 | 1.50E-55 | -1.69E-46 |
| MS.gene058010 | MS.gene49946 | 0.815005 | 1.15E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene49946 | 0.813195 | 2.89E-51 | -1.69E-46 |
| MS.gene058146 | MS.gene49946 | 0.800904 | 1.16E-48 | -1.69E-46 |
| MS.gene058548 | MS.gene49946 | 0.842787 | 2.09E-58 | -1.69E-46 |
| MS.gene058595 | MS.gene49946 | 0.806321 | 8.71E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene49946 | 0.814159 | 1.78E-51 | -1.69E-46 |
| MS.gene058781 | MS.gene49946 | 0.817316 | 3.51E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene49946 | 0.837928 | 3.89E-57 | -1.69E-46 |
| MS.gene058979 | MS.gene49946 | 0.839103 | 1.94E-57 | -1.69E-46 |
| MS.gene059283 | MS.gene49946 | 0.800307 | 1.53E-48 | -1.69E-46 |
| MS.gene05954 | MS.gene49946 | 0.817048 | 4.04E-52 | -1.69E-46 |
| MS.gene059625 | MS.gene49946 | 0.808136 | 3.59E-50 | -1.69E-46 |
| MS.gene05979 | MS.gene49946 | 0.830136 | 3.48E-55 | -1.69E-46 |
| MS.gene059842 | MS.gene49946 | 0.821426 | 4.06E-53 | -1.69E-46 |
| MS.gene060011 | MS.gene49946 | 0.828816 | 7.28E-55 | -1.69E-46 |
| MS.gene060310 | MS.gene49946 | 0.835211 | 1.91E-56 | -1.69E-46 |
| MS.gene060322 | MS.gene49946 | 0.819639 | 1.05E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene49946 | 0.803463 | 3.44E-49 | -1.69E-46 |
| MS.gene060429 | MS.gene49946 | 0.803613 | 3.21E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene49946 | 0.812577 | 3.95E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene49946 | 0.808174 | 3.53E-50 | -1.69E-46 |
| MS.gene060634 | MS.gene49946 | -0.825133 | 5.53E-54 | -1.69E-46 |
| MS.gene060802 | MS.gene49946 | 0.809169 | 2.16E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene49946 | 0.812593 | 3.92E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene49946 | 0.803911 | 2.78E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene49946 | 0.826357 | 2.84E-54 | -1.69E-46 |
| MS.gene060937 | MS.gene49946 | 0.81455 | 1.46E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49946.t1 | MTR_6g486270 | 97.600 | 375 | 4 | 3 | 1 | 375 | 1 | 370 | 0.0 | 729 |
| MS.gene49946.t1 | MTR_2g102227 | 51.754 | 228 | 98 | 5 | 132 | 354 | 273 | 493 | 8.34e-58 | 196 |
| MS.gene49946.t1 | MTR_8g022290 | 28.358 | 268 | 144 | 6 | 133 | 353 | 68 | 334 | 4.54e-21 | 94.0 |
| MS.gene49946.t1 | MTR_4g079960 | 37.190 | 121 | 58 | 4 | 119 | 222 | 5 | 124 | 8.48e-17 | 80.5 |
| MS.gene49946.t1 | MTR_4g079960 | 37.190 | 121 | 58 | 4 | 119 | 222 | 5 | 124 | 1.18e-16 | 80.1 |
| MS.gene49946.t1 | MTR_4g079960 | 37.190 | 121 | 58 | 4 | 119 | 222 | 5 | 124 | 1.53e-16 | 80.1 |
| MS.gene49946.t1 | MTR_1g061600 | 38.235 | 102 | 60 | 2 | 131 | 231 | 131 | 230 | 6.32e-16 | 79.3 |
| MS.gene49946.t1 | MTR_2g016140 | 35.106 | 94 | 56 | 2 | 133 | 222 | 23 | 115 | 3.42e-15 | 75.9 |
| MS.gene49946.t1 | MTR_2g016140 | 35.106 | 94 | 56 | 2 | 133 | 222 | 23 | 115 | 5.74e-15 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49946.t1 | AT3G24490 | 56.957 | 230 | 91 | 2 | 132 | 361 | 89 | 310 | 8.67e-83 | 256 |
| MS.gene49946.t1 | AT3G58630 | 27.055 | 292 | 156 | 7 | 115 | 353 | 7 | 294 | 4.18e-27 | 109 |
| MS.gene49946.t1 | AT3G58630 | 27.055 | 292 | 156 | 7 | 115 | 353 | 7 | 294 | 4.99e-27 | 109 |
| MS.gene49946.t1 | AT3G10030 | 33.149 | 181 | 109 | 4 | 47 | 225 | 82 | 252 | 1.65e-18 | 87.4 |
| MS.gene49946.t1 | AT3G10030 | 33.149 | 181 | 109 | 4 | 47 | 225 | 82 | 252 | 3.24e-18 | 86.7 |
| MS.gene49946.t1 | AT5G05550 | 32.468 | 231 | 122 | 7 | 133 | 351 | 24 | 232 | 9.12e-18 | 82.4 |
| MS.gene49946.t1 | AT5G05550 | 32.468 | 231 | 122 | 7 | 133 | 351 | 24 | 232 | 1.27e-17 | 82.0 |
| MS.gene49946.t1 | AT3G14180 | 37.405 | 131 | 65 | 4 | 104 | 227 | 58 | 178 | 1.65e-17 | 84.0 |
| MS.gene49946.t1 | AT5G05550 | 32.468 | 231 | 122 | 7 | 133 | 351 | 24 | 232 | 1.75e-17 | 81.6 |
| MS.gene49946.t1 | AT1G54060 | 28.846 | 260 | 140 | 8 | 133 | 351 | 94 | 349 | 1.10e-16 | 81.3 |
| MS.gene49946.t1 | AT3G11100 | 33.333 | 231 | 125 | 7 | 133 | 351 | 22 | 235 | 1.46e-16 | 79.0 |
| MS.gene49946.t1 | AT3G24860 | 35.870 | 92 | 53 | 1 | 133 | 224 | 65 | 150 | 6.45e-13 | 69.3 |
Find 88 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTCGATTCAGAAATT+TGG | 0.112221 | 6.4:+59550178 | MS.gene49946:CDS |
| GTTGCAGAAGAAGCAGATTT+TGG | 0.210644 | 6.4:+59550288 | MS.gene49946:CDS |
| TGGGATTAAGTTCTTGTAAA+TGG | 0.218020 | 6.4:+59549897 | MS.gene49946:CDS |
| GGAGGCTTCGTATAGGATTT+TGG | 0.224205 | 6.4:+59550156 | MS.gene49946:CDS |
| CTTGCTTGTGGTGTTGATTC+GGG | 0.238266 | 6.4:+59549977 | MS.gene49946:CDS |
| GGTGAGAAGTTTCTTCAAAT+TGG | 0.239028 | 6.4:+59549719 | MS.gene49946:CDS |
| CTGATTCGATTCAGAAATTT+GGG | 0.270259 | 6.4:+59550179 | MS.gene49946:CDS |
| CGTTTGTGTTGTTAGAGATA+TGG | 0.280545 | 6.4:+59549696 | MS.gene49946:CDS |
| AGTGGCCCTAGTGGCGGTTT+TGG | 0.306452 | 6.4:+59549539 | MS.gene49946:CDS |
| TCGAGTTCTGTCCATTCCTC+TGG | 0.317779 | 6.4:+59549647 | MS.gene49946:CDS |
| AGAATGGATGAGATGGGTTT+GGG | 0.330760 | 6.4:+59549878 | MS.gene49946:CDS |
| TGATATTGTAAGTTACGAAT+TGG | 0.332259 | 6.4:-59549368 | None:intergenic |
| ACTTGCTTGTGGTGTTGATT+CGG | 0.332404 | 6.4:+59549976 | MS.gene49946:CDS |
| GTTATAATCATCGAATTCTT+CGG | 0.353879 | 6.4:-59549432 | None:intergenic |
| TTATAATCATCGAATTCTTC+GGG | 0.363359 | 6.4:-59549431 | None:intergenic |
| GTGAGAAGTTTCTTCAAATT+GGG | 0.369920 | 6.4:+59549720 | MS.gene49946:CDS |
| TAGAATGGATGAGATGGGTT+TGG | 0.395377 | 6.4:+59549877 | MS.gene49946:CDS |
| AACGTAGATGAAGACGGAAT+TGG | 0.404212 | 6.4:+59549452 | MS.gene49946:CDS |
| GATGAAGACGGAATTGGAAA+TGG | 0.406005 | 6.4:+59549458 | MS.gene49946:CDS |
| GAAGAGTAGAATGGATGAGA+TGG | 0.406263 | 6.4:+59549871 | MS.gene49946:CDS |
| GTTTGTGTTGTTAGAGATAT+GGG | 0.412812 | 6.4:+59549697 | MS.gene49946:CDS |
| GATATTGTAAGTTACGAATT+GGG | 0.429997 | 6.4:-59549367 | None:intergenic |
| ATACCAGGGTTTATTTGAAT+AGG | 0.432946 | 6.4:+59550017 | MS.gene49946:CDS |
| CCACCACCAAAACCGCCACT+AGG | 0.438484 | 6.4:-59549545 | None:intergenic |
| AAGAGTAGAATGGATGAGAT+GGG | 0.457358 | 6.4:+59549872 | MS.gene49946:CDS |
| TAAGAAAGAGAAGAGTAGAA+TGG | 0.459638 | 6.4:+59549862 | MS.gene49946:CDS |
| TTATGAATTGTTGCCGCCAT+GGG | 0.464247 | 6.4:+59549586 | MS.gene49946:CDS |
| CTGAGGAGTTGAAAGTTGAA+AGG | 0.467561 | 6.4:+59549792 | MS.gene49946:CDS |
| GATTCAAGATACCCATCAAA+AGG | 0.470251 | 6.4:+59549302 | MS.gene49946:CDS |
| GGAGTTGGAGAAGGTGAGGT+TGG | 0.470646 | 6.4:+59550246 | MS.gene49946:CDS |
| GGCTGAAATATCGAAAATAC+AGG | 0.472979 | 6.4:+59550321 | MS.gene49946:CDS |
| GGAATTGGAAATGGGTATGT+TGG | 0.473631 | 6.4:+59549467 | MS.gene49946:CDS |
| CGAGTTCTGTCCATTCCTCT+GGG | 0.474330 | 6.4:+59549648 | MS.gene49946:CDS |
| TACTAAGAGGCAACAGATGA+TGG | 0.477066 | 6.4:+59550225 | MS.gene49946:CDS |
| ATGAAGACGGAATTGGAAAT+GGG | 0.477798 | 6.4:+59549459 | MS.gene49946:CDS |
| TCGAGTGCGAGACAAGAGTA+TGG | 0.478047 | 6.4:+59549953 | MS.gene49946:CDS |
| ATTCCTCTGGGTCGGAGGAA+TGG | 0.482329 | 6.4:+59549660 | MS.gene49946:CDS |
| TTCTGTCCATTCCTCTGGGT+CGG | 0.485623 | 6.4:+59549652 | MS.gene49946:CDS |
| GTGTAGGAGTGTATTGGATA+AGG | 0.493185 | 6.4:+59549826 | MS.gene49946:CDS |
| AAGAGGAGGAGGCTTCGTAT+AGG | 0.493270 | 6.4:+59550149 | MS.gene49946:CDS |
| GTGTATTGGATAAGGTGAAG+AGG | 0.499755 | 6.4:+59549834 | MS.gene49946:CDS |
| ATTATGAATTGTTGCCGCCA+TGG | 0.507987 | 6.4:+59549585 | MS.gene49946:CDS |
| GGATGATGTCGATAGGGAAG+AGG | 0.514505 | 6.4:+59550132 | MS.gene49946:CDS |
| GAATGGATTTGATGAGATGA+GGG | 0.516784 | 6.4:+59550042 | MS.gene49946:CDS |
| GAGGCAACAGATGATGGAGT+TGG | 0.516973 | 6.4:+59550231 | MS.gene49946:CDS |
| TGATGTCGATAGGGAAGAGG+AGG | 0.521958 | 6.4:+59550135 | MS.gene49946:CDS |
| TGTCGATAGGGAAGAGGAGG+AGG | 0.528458 | 6.4:+59550138 | MS.gene49946:CDS |
| CTTCAATTCAATGGACGACA+TGG | 0.528859 | 6.4:+59549274 | None:intergenic |
| AGAAGATTGAGAATACTAAG+AGG | 0.534145 | 6.4:+59550212 | MS.gene49946:CDS |
| CGAATGGATTTGATGAGATG+AGG | 0.535045 | 6.4:+59550041 | MS.gene49946:CDS |
| TTGCTTGTGGTGTTGATTCG+GGG | 0.537559 | 6.4:+59549978 | MS.gene49946:CDS |
| CACCACCAAAACCGCCACTA+GGG | 0.545654 | 6.4:-59549544 | None:intergenic |
| GGATGATGATGATGAGGATG+AGG | 0.549901 | 6.4:+59550093 | MS.gene49946:CDS |
| AGTATGTGTTTATGAATACC+AGG | 0.551827 | 6.4:+59550002 | MS.gene49946:CDS |
| ATTCCTCCGACCCAGAGGAA+TGG | 0.555282 | 6.4:-59549658 | None:intergenic |
| TTGTTGCCGCCATGGGGCAG+TGG | 0.558563 | 6.4:+59549593 | MS.gene49946:CDS |
| AGAAGTTTCTTCAAATTGGG+AGG | 0.559816 | 6.4:+59549723 | MS.gene49946:CDS |
| GTTTATTTGAATAGGTCGAA+TGG | 0.561187 | 6.4:+59550025 | MS.gene49946:CDS |
| GGCACAGTGTAGGAGTGTAT+TGG | 0.561474 | 6.4:+59549820 | MS.gene49946:CDS |
| ACAGATGATGGAGTTGGAGA+AGG | 0.562220 | 6.4:+59550237 | MS.gene49946:CDS |
| AAAGGAATGTGGCACAGTGT+AGG | 0.572005 | 6.4:+59549810 | MS.gene49946:CDS |
| ATTCAAGATACCCATCAAAA+GGG | 0.572324 | 6.4:+59549303 | MS.gene49946:CDS |
| TGTCCATTCCTCTGGGTCGG+AGG | 0.586004 | 6.4:+59549655 | MS.gene49946:CDS |
| GAGGAATAGTTTGAGATCAG+AGG | 0.586253 | 6.4:+59549742 | MS.gene49946:CDS |
| TTCTGAGGATGATGATGATG+AGG | 0.586254 | 6.4:+59550087 | MS.gene49946:CDS |
| TCAGAACTCTCAGACTCACC+AGG | 0.597701 | 6.4:-59550070 | None:intergenic |
| TGGTGAGTCTGAGAGTTCTG+AGG | 0.600777 | 6.4:+59550072 | MS.gene49946:CDS |
| TGATGAGGATGATGTCGATA+GGG | 0.605846 | 6.4:+59550126 | MS.gene49946:CDS |
| TGATGGAGTTGGAGAAGGTG+AGG | 0.617953 | 6.4:+59550242 | MS.gene49946:CDS |
| ATAGTTTGAGATCAGAGGAA+TGG | 0.618950 | 6.4:+59549747 | MS.gene49946:CDS |
| ATGCGAAGTGGCCCTAGTGG+CGG | 0.619751 | 6.4:+59549533 | MS.gene49946:CDS |
| GAAAATTTACCACTGCCCCA+TGG | 0.622030 | 6.4:-59549602 | None:intergenic |
| TGCTTGTGGTGTTGATTCGG+GGG | 0.626912 | 6.4:+59549979 | MS.gene49946:CDS |
| GTGATGAGGATGATGTCGAT+AGG | 0.628162 | 6.4:+59550125 | MS.gene49946:CDS |
| AGTTGCTGAGAAAGTTTCTG+AGG | 0.628405 | 6.4:+59549775 | MS.gene49946:CDS |
| CGACCTATTCAAATAAACCC+TGG | 0.636066 | 6.4:-59550020 | None:intergenic |
| GATTATAACGTAGATGAAGA+CGG | 0.641525 | 6.4:+59549446 | MS.gene49946:CDS |
| TATGAATTGTTGCCGCCATG+GGG | 0.651070 | 6.4:+59549587 | MS.gene49946:CDS |
| GATGAGATGAGGGATAGTCC+TGG | 0.652387 | 6.4:+59550052 | MS.gene49946:CDS |
| CAAGAGTATGGACTTGCTTG+TGG | 0.652939 | 6.4:+59549965 | MS.gene49946:CDS |
| GTTGAAAGTTGAAAGGAATG+TGG | 0.654797 | 6.4:+59549799 | MS.gene49946:CDS |
| TTTGTGTTGTTAGAGATATG+GGG | 0.660660 | 6.4:+59549698 | MS.gene49946:CDS |
| TGATGCTGATGGTGATGATG+AGG | 0.663076 | 6.4:+59550354 | MS.gene49946:CDS |
| GTATGTGTTTATGAATACCA+GGG | 0.664776 | 6.4:+59550003 | MS.gene49946:CDS |
| AAAATGCGAAGTGGCCCTAG+TGG | 0.671315 | 6.4:+59549530 | MS.gene49946:CDS |
| TGTCCATTCCTCCGACCCAG+AGG | 0.680725 | 6.4:-59549663 | None:intergenic |
| GAAGCAGTAGATGATGCTGA+TGG | 0.735151 | 6.4:+59550343 | MS.gene49946:CDS |
| AATTTACCACTGCCCCATGG+CGG | 0.737952 | 6.4:-59549599 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTTTGAAGAAAATGTT+GGG | + | chr6.4:59549489-59549508 | MS.gene49946:CDS | 20.0% |
| ! | ATATCCATCTTCTTGAAAAA+TGG | - | chr6.4:59549923-59549942 | None:intergenic | 25.0% |
| ! | GATATTGTAAGTTACGAATT+GGG | - | chr6.4:59549370-59549389 | None:intergenic | 25.0% |
| ! | GTTATAATCATCGAATTCTT+CGG | - | chr6.4:59549435-59549454 | None:intergenic | 25.0% |
| ! | TGATATTGTAAGTTACGAAT+TGG | - | chr6.4:59549371-59549390 | None:intergenic | 25.0% |
| ! | TTATAATCATCGAATTCTTC+GGG | - | chr6.4:59549434-59549453 | None:intergenic | 25.0% |
| !! | AAATTTTCGAACTTTAATCG+TGG | + | chr6.4:59549617-59549636 | MS.gene49946:CDS | 25.0% |
| !!! | GGAAATTTTGAAGAAAATGT+TGG | + | chr6.4:59549488-59549507 | MS.gene49946:CDS | 25.0% |
| AAAAAGAGAAAAATGCGAAG+TGG | + | chr6.4:59549521-59549540 | MS.gene49946:CDS | 30.0% | |
| AGAAGATTGAGAATACTAAG+AGG | + | chr6.4:59550212-59550231 | MS.gene49946:CDS | 30.0% | |
| AGTATGTGTTTATGAATACC+AGG | + | chr6.4:59550002-59550021 | MS.gene49946:CDS | 30.0% | |
| ATACCAGGGTTTATTTGAAT+AGG | + | chr6.4:59550017-59550036 | MS.gene49946:CDS | 30.0% | |
| ATTCAAGATACCCATCAAAA+GGG | + | chr6.4:59549303-59549322 | MS.gene49946:CDS | 30.0% | |
| GATTATAACGTAGATGAAGA+CGG | + | chr6.4:59549446-59549465 | MS.gene49946:CDS | 30.0% | |
| GTATGTGTTTATGAATACCA+GGG | + | chr6.4:59550003-59550022 | MS.gene49946:CDS | 30.0% | |
| GTGAGAAGTTTCTTCAAATT+GGG | + | chr6.4:59549720-59549739 | MS.gene49946:CDS | 30.0% | |
| GTTTATTTGAATAGGTCGAA+TGG | + | chr6.4:59550025-59550044 | MS.gene49946:CDS | 30.0% | |
| TAAGAAAGAGAAGAGTAGAA+TGG | + | chr6.4:59549862-59549881 | MS.gene49946:CDS | 30.0% | |
| TGGGATTAAGTTCTTGTAAA+TGG | + | chr6.4:59549897-59549916 | MS.gene49946:CDS | 30.0% | |
| ! | CTGATTCGATTCAGAAATTT+GGG | + | chr6.4:59550179-59550198 | MS.gene49946:CDS | 30.0% |
| ! | GTTTGTGTTGTTAGAGATAT+GGG | + | chr6.4:59549697-59549716 | MS.gene49946:CDS | 30.0% |
| ! | TTTGTGTTGTTAGAGATATG+GGG | + | chr6.4:59549698-59549717 | MS.gene49946:CDS | 30.0% |
| !! | GTTGGATTTTAATAGGGAAT+TGG | + | chr6.4:59550264-59550283 | MS.gene49946:CDS | 30.0% |
| AAGAGTAGAATGGATGAGAT+GGG | + | chr6.4:59549872-59549891 | MS.gene49946:CDS | 35.0% | |
| AGAAGTTTCTTCAAATTGGG+AGG | + | chr6.4:59549723-59549742 | MS.gene49946:CDS | 35.0% | |
| ATGAAGACGGAATTGGAAAT+GGG | + | chr6.4:59549459-59549478 | MS.gene49946:CDS | 35.0% | |
| GATTCAAGATACCCATCAAA+AGG | + | chr6.4:59549302-59549321 | MS.gene49946:CDS | 35.0% | |
| GGCTGAAATATCGAAAATAC+AGG | + | chr6.4:59550321-59550340 | MS.gene49946:CDS | 35.0% | |
| GGTGAGAAGTTTCTTCAAAT+TGG | + | chr6.4:59549719-59549738 | MS.gene49946:CDS | 35.0% | |
| GTTGAAAGTTGAAAGGAATG+TGG | + | chr6.4:59549799-59549818 | MS.gene49946:CDS | 35.0% | |
| ! | AGAAGAAGCAGATTTTGGAT+AGG | + | chr6.4:59550293-59550312 | MS.gene49946:CDS | 35.0% |
| ! | ATAGTTTGAGATCAGAGGAA+TGG | + | chr6.4:59549747-59549766 | MS.gene49946:CDS | 35.0% |
| ! | CGTTTGTGTTGTTAGAGATA+TGG | + | chr6.4:59549696-59549715 | MS.gene49946:CDS | 35.0% |
| ! | GAAGAAGCAGATTTTGGATA+GGG | + | chr6.4:59550294-59550313 | MS.gene49946:CDS | 35.0% |
| ! | GAATGGATTTGATGAGATGA+GGG | + | chr6.4:59550042-59550061 | MS.gene49946:CDS | 35.0% |
| ! | TTTTCGAACTTTAATCGTGG+TGG | + | chr6.4:59549620-59549639 | MS.gene49946:CDS | 35.0% |
| !! | AGGTGAGGTTGGATTTTAAT+AGG | + | chr6.4:59550257-59550276 | MS.gene49946:CDS | 35.0% |
| !! | GCTGATTCGATTCAGAAATT+TGG | + | chr6.4:59550178-59550197 | MS.gene49946:CDS | 35.0% |
| !! | GGTGAGGTTGGATTTTAATA+GGG | + | chr6.4:59550258-59550277 | MS.gene49946:CDS | 35.0% |
| !! | GGTGTTTTTGACGAAGATTA+GGG | - | chr6.4:59549349-59549368 | None:intergenic | 35.0% |
| !!! | ATGGCCATTTTTCAAGAAGA+TGG | + | chr6.4:59549916-59549935 | MS.gene49946:CDS | 35.0% |
| AACGTAGATGAAGACGGAAT+TGG | + | chr6.4:59549452-59549471 | MS.gene49946:CDS | 40.0% | |
| AGTTGCTGAGAAAGTTTCTG+AGG | + | chr6.4:59549775-59549794 | MS.gene49946:CDS | 40.0% | |
| ATTATGAATTGTTGCCGCCA+TGG | + | chr6.4:59549585-59549604 | MS.gene49946:CDS | 40.0% | |
| CGACCTATTCAAATAAACCC+TGG | - | chr6.4:59550023-59550042 | None:intergenic | 40.0% | |
| CTGAGGAGTTGAAAGTTGAA+AGG | + | chr6.4:59549792-59549811 | MS.gene49946:CDS | 40.0% | |
| GAAGAGTAGAATGGATGAGA+TGG | + | chr6.4:59549871-59549890 | MS.gene49946:CDS | 40.0% | |
| GATGAAGACGGAATTGGAAA+TGG | + | chr6.4:59549458-59549477 | MS.gene49946:CDS | 40.0% | |
| GGAATTGGAAATGGGTATGT+TGG | + | chr6.4:59549467-59549486 | MS.gene49946:CDS | 40.0% | |
| GTGTATTGGATAAGGTGAAG+AGG | + | chr6.4:59549834-59549853 | MS.gene49946:CDS | 40.0% | |
| TACTAAGAGGCAACAGATGA+TGG | + | chr6.4:59550225-59550244 | MS.gene49946:CDS | 40.0% | |
| TGATGAGGATGATGTCGATA+GGG | + | chr6.4:59550126-59550145 | MS.gene49946:CDS | 40.0% | |
| TTATGAATTGTTGCCGCCAT+GGG | + | chr6.4:59549586-59549605 | MS.gene49946:CDS | 40.0% | |
| ! | AGAATGGATGAGATGGGTTT+GGG | + | chr6.4:59549878-59549897 | MS.gene49946:CDS | 40.0% |
| ! | CGAATGGATTTGATGAGATG+AGG | + | chr6.4:59550041-59550060 | MS.gene49946:CDS | 40.0% |
| ! | GAGGAATAGTTTGAGATCAG+AGG | + | chr6.4:59549742-59549761 | MS.gene49946:CDS | 40.0% |
| ! | GATGAGGATGAGGATTTTGA+TGG | + | chr6.4:59550103-59550122 | MS.gene49946:CDS | 40.0% |
| ! | GTGTAGGAGTGTATTGGATA+AGG | + | chr6.4:59549826-59549845 | MS.gene49946:CDS | 40.0% |
| ! | GTTGCAGAAGAAGCAGATTT+TGG | + | chr6.4:59550288-59550307 | MS.gene49946:CDS | 40.0% |
| ! | TAGAATGGATGAGATGGGTT+TGG | + | chr6.4:59549877-59549896 | MS.gene49946:CDS | 40.0% |
| ! | TAGGGTTTTTGCGTCGTAAA+GGG | - | chr6.4:59549331-59549350 | None:intergenic | 40.0% |
| ! | TTAGGGTTTTTGCGTCGTAA+AGG | - | chr6.4:59549332-59549351 | None:intergenic | 40.0% |
| ! | TTCTGAGGATGATGATGATG+AGG | + | chr6.4:59550087-59550106 | MS.gene49946:CDS | 40.0% |
| !! | ACTTGCTTGTGGTGTTGATT+CGG | + | chr6.4:59549976-59549995 | MS.gene49946:CDS | 40.0% |
| !! | GGGTGTTTTTGACGAAGATT+AGG | - | chr6.4:59549350-59549369 | None:intergenic | 40.0% |
| !!! | GTAAAGGGTACCCTTTTGAT+GGG | - | chr6.4:59549316-59549335 | None:intergenic | 40.0% |
| !!! | TGAGGATTTTGATGGTGATG+AGG | + | chr6.4:59550111-59550130 | MS.gene49946:CDS | 40.0% |
| AAAGGAATGTGGCACAGTGT+AGG | + | chr6.4:59549810-59549829 | MS.gene49946:CDS | 45.0% | |
| GAAAATTTACCACTGCCCCA+TGG | - | chr6.4:59549605-59549624 | None:intergenic | 45.0% | |
| GAAGCAGTAGATGATGCTGA+TGG | + | chr6.4:59550343-59550362 | MS.gene49946:CDS | 45.0% | |
| GTGATGAGGATGATGTCGAT+AGG | + | chr6.4:59550125-59550144 | MS.gene49946:CDS | 45.0% | |
| TATGAATTGTTGCCGCCATG+GGG | + | chr6.4:59549587-59549606 | MS.gene49946:CDS | 45.0% | |
| ! | CAAGAGTATGGACTTGCTTG+TGG | + | chr6.4:59549965-59549984 | MS.gene49946:CDS | 45.0% |
| ! | GGAGGCTTCGTATAGGATTT+TGG | + | chr6.4:59550156-59550175 | MS.gene49946:CDS | 45.0% |
| ! | GGATGATGATGATGAGGATG+AGG | + | chr6.4:59550093-59550112 | MS.gene49946:CDS | 45.0% |
| ! | TGATGCTGATGGTGATGATG+AGG | + | chr6.4:59550354-59550373 | MS.gene49946:CDS | 45.0% |
| !! | ACAGATGATGGAGTTGGAGA+AGG | + | chr6.4:59550237-59550256 | MS.gene49946:CDS | 45.0% |
| !! | CTTGCTTGTGGTGTTGATTC+GGG | + | chr6.4:59549977-59549996 | MS.gene49946:CDS | 45.0% |
| !! | TTGCTTGTGGTGTTGATTCG+GGG | + | chr6.4:59549978-59549997 | MS.gene49946:CDS | 45.0% |
| !!! | CGTAAAGGGTACCCTTTTGA+TGG | - | chr6.4:59549317-59549336 | None:intergenic | 45.0% |
| AAAATGCGAAGTGGCCCTAG+TGG | + | chr6.4:59549530-59549549 | MS.gene49946:CDS | 50.0% | |
| AAGAGGAGGAGGCTTCGTAT+AGG | + | chr6.4:59550149-59550168 | MS.gene49946:CDS | 50.0% | |
| AATTTACCACTGCCCCATGG+CGG | - | chr6.4:59549602-59549621 | None:intergenic | 50.0% | |
| CGAGTTCTGTCCATTCCTCT+GGG | + | chr6.4:59549648-59549667 | MS.gene49946:CDS | 50.0% | |
| GATGAGATGAGGGATAGTCC+TGG | + | chr6.4:59550052-59550071 | MS.gene49946:CDS | 50.0% | |
| GGATGATGTCGATAGGGAAG+AGG | + | chr6.4:59550132-59550151 | MS.gene49946:CDS | 50.0% | |
| TCAGAACTCTCAGACTCACC+AGG | - | chr6.4:59550073-59550092 | None:intergenic | 50.0% | |
| TCGAGTGCGAGACAAGAGTA+TGG | + | chr6.4:59549953-59549972 | MS.gene49946:CDS | 50.0% | |
| TCGAGTTCTGTCCATTCCTC+TGG | + | chr6.4:59549647-59549666 | MS.gene49946:CDS | 50.0% | |
| TGATGGAGTTGGAGAAGGTG+AGG | + | chr6.4:59550242-59550261 | MS.gene49946:CDS | 50.0% | |
| TGATGTCGATAGGGAAGAGG+AGG | + | chr6.4:59550135-59550154 | MS.gene49946:CDS | 50.0% | |
| TGGTGAGTCTGAGAGTTCTG+AGG | + | chr6.4:59550072-59550091 | MS.gene49946:CDS | 50.0% | |
| TTCTGTCCATTCCTCTGGGT+CGG | + | chr6.4:59549652-59549671 | MS.gene49946:CDS | 50.0% | |
| ! | GCAGATTTTGGATAGGGCTC+AGG | + | chr6.4:59550300-59550319 | MS.gene49946:CDS | 50.0% |
| ! | GGCACAGTGTAGGAGTGTAT+TGG | + | chr6.4:59549820-59549839 | MS.gene49946:CDS | 50.0% |
| !! | GAGGCAACAGATGATGGAGT+TGG | + | chr6.4:59550231-59550250 | MS.gene49946:CDS | 50.0% |
| !! | TGCTTGTGGTGTTGATTCGG+GGG | + | chr6.4:59549979-59549998 | MS.gene49946:CDS | 50.0% |
| ATTCCTCCGACCCAGAGGAA+TGG | - | chr6.4:59549661-59549680 | None:intergenic | 55.0% | |
| ATTCCTCTGGGTCGGAGGAA+TGG | + | chr6.4:59549660-59549679 | MS.gene49946:CDS | 55.0% | |
| CACCACCAAAACCGCCACTA+GGG | - | chr6.4:59549547-59549566 | None:intergenic | 55.0% | |
| GGAGTTGGAGAAGGTGAGGT+TGG | + | chr6.4:59550246-59550265 | MS.gene49946:CDS | 55.0% | |
| TGTCGATAGGGAAGAGGAGG+AGG | + | chr6.4:59550138-59550157 | MS.gene49946:CDS | 55.0% | |
| ATGCGAAGTGGCCCTAGTGG+CGG | + | chr6.4:59549533-59549552 | MS.gene49946:CDS | 60.0% | |
| CCACCACCAAAACCGCCACT+AGG | - | chr6.4:59549548-59549567 | None:intergenic | 60.0% | |
| TGTCCATTCCTCCGACCCAG+AGG | - | chr6.4:59549666-59549685 | None:intergenic | 60.0% | |
| TGTCCATTCCTCTGGGTCGG+AGG | + | chr6.4:59549655-59549674 | MS.gene49946:CDS | 60.0% | |
| ! | AGTGGCCCTAGTGGCGGTTT+TGG | + | chr6.4:59549539-59549558 | MS.gene49946:CDS | 60.0% |
| !! | CCTAGTGGCGGTTTTGGTGG+TGG | + | chr6.4:59549545-59549564 | MS.gene49946:CDS | 60.0% |
| !!! | AGTGGCGGTTTTGGTGGTGG+TGG | + | chr6.4:59549548-59549567 | MS.gene49946:CDS | 60.0% |
| TTGTTGCCGCCATGGGGCAG+TGG | + | chr6.4:59549593-59549612 | MS.gene49946:CDS | 65.0% | |
| !! | GGCCCTAGTGGCGGTTTTGG+TGG | + | chr6.4:59549542-59549561 | MS.gene49946:CDS | 65.0% |
| !!! | GGCGGTTTTGGTGGTGGTGG+TGG | + | chr6.4:59549551-59549570 | MS.gene49946:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 59549284 | 59550411 | 59549284 | ID=MS.gene49946 |
| chr6.4 | mRNA | 59549284 | 59550411 | 59549284 | ID=MS.gene49946.t1;Parent=MS.gene49946 |
| chr6.4 | exon | 59549284 | 59550411 | 59549284 | ID=MS.gene49946.t1.exon1;Parent=MS.gene49946.t1 |
| chr6.4 | CDS | 59549284 | 59550411 | 59549284 | ID=cds.MS.gene49946.t1;Parent=MS.gene49946.t1 |
| Gene Sequence |
| Protein sequence |
