Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29921.t1 | GAU17568.1 | 64.1 | 334 | 105 | 5 | 1 | 330 | 404 | 726 | 4.60E-107 | 397.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29921.t1 | Q9FPT1 | 46.2 | 130 | 67 | 3 | 10 | 138 | 56 | 183 | 6.7e-22 | 106.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29921.t1 | A0A392MFG0 | 65.3 | 334 | 101 | 5 | 1 | 330 | 4 | 326 | 5.0e-111 | 410.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene29921 | 0.821535 | 3.83E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene29921 | 0.818011 | 2.45E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene29921 | 0.80787 | 4.09E-50 | -1.69E-46 |
| MS.gene049807 | MS.gene29921 | 0.828317 | 9.61E-55 | -1.69E-46 |
| MS.gene049808 | MS.gene29921 | 0.808348 | 3.24E-50 | -1.69E-46 |
| MS.gene050237 | MS.gene29921 | 0.826811 | 2.21E-54 | -1.69E-46 |
| MS.gene050273 | MS.gene29921 | 0.802062 | 6.70E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene29921 | 0.812536 | 4.04E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene29921 | 0.835497 | 1.62E-56 | -1.69E-46 |
| MS.gene050561 | MS.gene29921 | 0.828118 | 1.07E-54 | -1.69E-46 |
| MS.gene050562 | MS.gene29921 | 0.820557 | 6.44E-53 | -1.69E-46 |
| MS.gene050799 | MS.gene29921 | 0.805628 | 1.22E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene29921 | 0.839111 | 1.93E-57 | -1.69E-46 |
| MS.gene051699 | MS.gene29921 | 0.810251 | 1.26E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene29921 | 0.809507 | 1.83E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene29921 | 0.817693 | 2.89E-52 | -1.69E-46 |
| MS.gene052239 | MS.gene29921 | -0.839855 | 1.23E-57 | -1.69E-46 |
| MS.gene052447 | MS.gene29921 | 0.831522 | 1.59E-55 | -1.69E-46 |
| MS.gene052516 | MS.gene29921 | 0.81591 | 7.26E-52 | -1.69E-46 |
| MS.gene052519 | MS.gene29921 | 0.830212 | 3.34E-55 | -1.69E-46 |
| MS.gene052582 | MS.gene29921 | 0.810862 | 9.33E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene29921 | 0.814706 | 1.34E-51 | -1.69E-46 |
| MS.gene052793 | MS.gene29921 | 0.800458 | 1.43E-48 | -1.69E-46 |
| MS.gene053082 | MS.gene29921 | 0.819855 | 9.33E-53 | -1.69E-46 |
| MS.gene053599 | MS.gene29921 | 0.819205 | 1.31E-52 | -1.69E-46 |
| MS.gene05364 | MS.gene29921 | 0.80685 | 6.73E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene29921 | 0.809811 | 1.57E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene29921 | 0.813356 | 2.67E-51 | -1.69E-46 |
| MS.gene055464 | MS.gene29921 | 0.801626 | 8.24E-49 | -1.69E-46 |
| MS.gene055468 | MS.gene29921 | 0.801974 | 6.99E-49 | -1.69E-46 |
| MS.gene055580 | MS.gene29921 | -0.80251 | 5.42E-49 | -1.69E-46 |
| MS.gene055724 | MS.gene29921 | 0.813235 | 2.84E-51 | -1.69E-46 |
| MS.gene055840 | MS.gene29921 | 0.807571 | 4.74E-50 | -1.69E-46 |
| MS.gene056082 | MS.gene29921 | 0.807324 | 5.34E-50 | -1.69E-46 |
| MS.gene056174 | MS.gene29921 | 0.80006 | 1.72E-48 | -1.69E-46 |
| MS.gene056272 | MS.gene29921 | 0.829455 | 5.10E-55 | -1.69E-46 |
| MS.gene056308 | MS.gene29921 | 0.823726 | 1.19E-53 | -1.69E-46 |
| MS.gene056366 | MS.gene29921 | 0.815711 | 8.04E-52 | -1.69E-46 |
| MS.gene056595 | MS.gene29921 | 0.806415 | 8.32E-50 | -1.69E-46 |
| MS.gene056677 | MS.gene29921 | 0.811568 | 6.56E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene29921 | 0.813383 | 2.63E-51 | -1.69E-46 |
| MS.gene057287 | MS.gene29921 | 0.804569 | 2.03E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene29921 | 0.815999 | 6.93E-52 | -1.69E-46 |
| MS.gene057694 | MS.gene29921 | 0.800587 | 1.34E-48 | -1.69E-46 |
| MS.gene057932 | MS.gene29921 | 0.801895 | 7.25E-49 | -1.69E-46 |
| MS.gene058008 | MS.gene29921 | 0.833172 | 6.21E-56 | -1.69E-46 |
| MS.gene058009 | MS.gene29921 | 0.835515 | 1.60E-56 | -1.69E-46 |
| MS.gene058330 | MS.gene29921 | 0.807307 | 5.39E-50 | -1.69E-46 |
| MS.gene059015 | MS.gene29921 | 0.834141 | 3.56E-56 | -1.69E-46 |
| MS.gene059625 | MS.gene29921 | 0.803836 | 2.88E-49 | -1.69E-46 |
| MS.gene060634 | MS.gene29921 | -0.801137 | 1.04E-48 | -1.69E-46 |
| MS.gene061100 | MS.gene29921 | 0.818142 | 2.29E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene057066 | MS.gene29921 | PPI |
| MS.gene030037 | MS.gene29921 | PPI |
| MS.gene017099 | MS.gene29921 | PPI |
| MS.gene057069 | MS.gene29921 | PPI |
| MS.gene038323 | MS.gene29921 | PPI |
| MS.gene038333 | MS.gene29921 | PPI |
| MS.gene29921 | MS.gene017083 | PPI |
| MS.gene29921 | MS.gene017100 | PPI |
| MS.gene030029 | MS.gene29921 | PPI |
| MS.gene29921 | MS.gene030026 | PPI |
| MS.gene038330 | MS.gene29921 | PPI |
| MS.gene29921 | MS.gene057065 | PPI |
| MS.gene017101 | MS.gene29921 | PPI |
| MS.gene038342 | MS.gene29921 | PPI |
| MS.gene030034 | MS.gene29921 | PPI |
| MS.gene29921 | MS.gene038324 | PPI |
| MS.gene038300 | MS.gene29921 | PPI |
| MS.gene017102 | MS.gene29921 | PPI |
| MS.gene030042 | MS.gene29921 | PPI |
| MS.gene030033 | MS.gene29921 | PPI |
| MS.gene29921 | MS.gene017115 | PPI |
| MS.gene038326 | MS.gene29921 | PPI |
| MS.gene038328 | MS.gene29921 | PPI |
| MS.gene057090 | MS.gene29921 | PPI |
| MS.gene038336 | MS.gene29921 | PPI |
| MS.gene29921 | MS.gene038327 | PPI |
| MS.gene29921 | MS.gene057070 | PPI |
| MS.gene29921 | MS.gene030045 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29921.t1 | MTR_7g107200 | 45.867 | 375 | 157 | 6 | 1 | 329 | 1 | 375 | 4.60e-108 | 320 |
| MS.gene29921.t1 | MTR_1g042160 | 49.701 | 334 | 136 | 8 | 1 | 328 | 1 | 308 | 2.54e-100 | 298 |
| MS.gene29921.t1 | MTR_7g107160 | 53.687 | 339 | 118 | 12 | 9 | 330 | 129 | 445 | 1.45e-99 | 301 |
| MS.gene29921.t1 | MTR_7g107160 | 49.612 | 129 | 45 | 3 | 66 | 174 | 1 | 129 | 7.43e-31 | 121 |
| MS.gene29921.t1 | MTR_8g011730 | 37.079 | 356 | 174 | 11 | 9 | 329 | 13 | 353 | 5.16e-63 | 204 |
| MS.gene29921.t1 | MTR_7g107170 | 54.037 | 161 | 66 | 1 | 168 | 328 | 111 | 263 | 2.14e-49 | 166 |
| MS.gene29921.t1 | MTR_7g107170 | 73.563 | 87 | 23 | 0 | 233 | 319 | 1 | 87 | 8.71e-33 | 122 |
| MS.gene29921.t1 | MTR_2g034780 | 36.747 | 166 | 102 | 1 | 158 | 323 | 99 | 261 | 4.68e-29 | 113 |
| MS.gene29921.t1 | MTR_3g079840 | 40.845 | 142 | 78 | 4 | 1 | 138 | 44 | 183 | 5.03e-26 | 109 |
| MS.gene29921.t1 | MTR_3g079840 | 40.845 | 142 | 78 | 4 | 1 | 138 | 45 | 184 | 5.22e-26 | 109 |
| MS.gene29921.t1 | MTR_5g086700 | 39.552 | 134 | 78 | 3 | 4 | 136 | 55 | 186 | 9.44e-26 | 108 |
| MS.gene29921.t1 | MTR_5g086700 | 39.552 | 134 | 78 | 3 | 4 | 136 | 55 | 186 | 1.04e-25 | 108 |
| MS.gene29921.t1 | MTR_1g041240 | 42.308 | 130 | 71 | 3 | 10 | 138 | 59 | 185 | 1.14e-24 | 105 |
| MS.gene29921.t1 | MTR_1g041240 | 42.308 | 130 | 71 | 3 | 10 | 138 | 58 | 184 | 1.15e-24 | 105 |
| MS.gene29921.t1 | MTR_1g041240 | 42.308 | 130 | 71 | 3 | 10 | 138 | 57 | 183 | 1.20e-24 | 105 |
| MS.gene29921.t1 | MTR_5g091450 | 38.095 | 126 | 76 | 2 | 10 | 134 | 33 | 157 | 2.39e-23 | 101 |
| MS.gene29921.t1 | MTR_5g091180 | 35.570 | 149 | 89 | 4 | 1 | 145 | 34 | 179 | 5.90e-21 | 94.4 |
| MS.gene29921.t1 | MTR_5g091550 | 34.646 | 127 | 80 | 3 | 9 | 134 | 18 | 142 | 1.00e-15 | 78.2 |
| MS.gene29921.t1 | MTR_5g091550 | 34.646 | 127 | 80 | 3 | 9 | 134 | 18 | 142 | 1.18e-15 | 77.8 |
| MS.gene29921.t1 | MTR_3g106700 | 34.752 | 141 | 82 | 3 | 10 | 140 | 406 | 546 | 9.43e-14 | 72.8 |
| MS.gene29921.t1 | MTR_1g073800 | 32.824 | 131 | 83 | 5 | 10 | 138 | 49 | 176 | 4.22e-12 | 67.0 |
| MS.gene29921.t1 | MTR_1g044450 | 33.588 | 131 | 80 | 5 | 10 | 138 | 65 | 190 | 1.51e-11 | 65.9 |
| MS.gene29921.t1 | MTR_4g123750 | 25.652 | 230 | 137 | 7 | 5 | 222 | 14 | 221 | 6.02e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29921.t1 | AT5G06600 | 44.366 | 142 | 73 | 4 | 1 | 138 | 44 | 183 | 9.98e-27 | 111 |
| MS.gene29921.t1 | AT5G06600 | 44.366 | 142 | 73 | 4 | 1 | 138 | 43 | 182 | 1.05e-26 | 111 |
| MS.gene29921.t1 | AT5G06600 | 44.366 | 142 | 73 | 4 | 1 | 138 | 44 | 183 | 1.17e-26 | 111 |
| MS.gene29921.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 55 | 182 | 2.69e-25 | 107 |
| MS.gene29921.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 55 | 182 | 2.69e-25 | 107 |
| MS.gene29921.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 54 | 181 | 2.75e-25 | 107 |
| MS.gene29921.t1 | AT3G11910 | 44.615 | 130 | 69 | 3 | 10 | 138 | 54 | 181 | 2.75e-25 | 107 |
| MS.gene29921.t1 | AT3G58340 | 25.517 | 290 | 168 | 9 | 5 | 268 | 3 | 270 | 2.81e-16 | 78.6 |
| MS.gene29921.t1 | AT3G58340 | 24.667 | 300 | 168 | 9 | 5 | 268 | 3 | 280 | 2.50e-15 | 76.3 |
| MS.gene29921.t1 | AT3G58220 | 25.822 | 213 | 140 | 6 | 11 | 218 | 13 | 212 | 9.84e-15 | 74.3 |
| MS.gene29921.t1 | AT3G58370 | 28.378 | 222 | 134 | 9 | 1 | 218 | 1 | 201 | 5.90e-14 | 71.6 |
| MS.gene29921.t1 | AT3G58220 | 23.469 | 294 | 174 | 7 | 11 | 259 | 13 | 300 | 7.33e-14 | 72.0 |
| MS.gene29921.t1 | AT3G58360 | 27.083 | 192 | 119 | 6 | 9 | 199 | 7 | 178 | 1.77e-13 | 70.5 |
| MS.gene29921.t1 | AT3G44790 | 25.197 | 254 | 175 | 7 | 9 | 258 | 4 | 246 | 2.96e-13 | 70.1 |
| MS.gene29921.t1 | AT3G58240 | 24.768 | 323 | 206 | 10 | 8 | 319 | 6 | 302 | 3.49e-13 | 69.7 |
| MS.gene29921.t1 | AT3G58220 | 27.072 | 181 | 130 | 2 | 11 | 190 | 13 | 192 | 7.10e-13 | 69.7 |
| MS.gene29921.t1 | AT3G58220 | 27.072 | 181 | 130 | 2 | 11 | 190 | 13 | 192 | 1.35e-12 | 68.2 |
| MS.gene29921.t1 | AT3G58220 | 30.469 | 128 | 85 | 3 | 11 | 135 | 13 | 139 | 2.04e-12 | 66.6 |
| MS.gene29921.t1 | AT3G27040 | 28.467 | 137 | 96 | 2 | 7 | 142 | 6 | 141 | 2.88e-12 | 66.6 |
| MS.gene29921.t1 | AT2G25320 | 32.278 | 158 | 96 | 4 | 10 | 157 | 405 | 561 | 4.85e-12 | 67.8 |
| MS.gene29921.t1 | AT2G25320 | 32.278 | 158 | 96 | 4 | 10 | 157 | 404 | 560 | 4.90e-12 | 67.8 |
| MS.gene29921.t1 | AT2G25320 | 32.278 | 158 | 96 | 4 | 10 | 157 | 405 | 561 | 1.04e-11 | 66.6 |
| MS.gene29921.t1 | AT2G25330 | 30.729 | 192 | 122 | 5 | 10 | 190 | 359 | 550 | 1.16e-11 | 66.2 |
| MS.gene29921.t1 | AT3G58210 | 32.824 | 131 | 85 | 3 | 8 | 136 | 6 | 135 | 1.83e-11 | 64.7 |
Find 62 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGCAAGTAAGGATTATT+TGG | 0.155785 | 8.3:-45856048 | MS.gene29921:CDS |
| GTACACTGCTAATATGTTTC+AGG | 0.178138 | 8.3:-45857096 | MS.gene29921:CDS |
| CCAAAGTCTCGACCACATTT+TGG | 0.217526 | 8.3:+45855844 | None:intergenic |
| CAAGTCAAATTGAAATTAAA+TGG | 0.219404 | 8.3:+45855594 | None:intergenic |
| TTCACAATAAACCCTCTTTC+AGG | 0.229550 | 8.3:+45855990 | None:intergenic |
| TTTGGAAAAGAACAGTTATT+TGG | 0.252160 | 8.3:-45855547 | MS.gene29921:CDS |
| CTCTCACCTTTCTTGTCTTA+AGG | 0.269117 | 8.3:+45855670 | None:intergenic |
| CGTTGGCCTTCTTCTAGTTC+TGG | 0.287736 | 8.3:+45855867 | None:intergenic |
| GAATGGGCGTTCACCGCTTT+GGG | 0.323269 | 8.3:-45855708 | MS.gene29921:CDS |
| GGAATGGGCGTTCACCGCTT+TGG | 0.324343 | 8.3:-45855709 | MS.gene29921:CDS |
| AGTAAGGATTATTTGGGTTT+CGG | 0.339740 | 8.3:-45856041 | MS.gene29921:CDS |
| TTCCTCTAGTAATGGAATAA+AGG | 0.345498 | 8.3:+45855791 | None:intergenic |
| GTGAAGGATGCTTGTATTGT+TGG | 0.355564 | 8.3:-45855972 | MS.gene29921:CDS |
| GCCTTGTGTTCAATCTGCTT+TGG | 0.362659 | 8.3:-45855565 | MS.gene29921:CDS |
| GGATGATTTGTCTGTATCAA+TGG | 0.368177 | 8.3:-45857163 | MS.gene29921:CDS |
| TACTCTGAGCCTTTCATCCT+TGG | 0.374548 | 8.3:-45857279 | MS.gene29921:CDS |
| TCTCTCACTTGAATAAGATT+AGG | 0.386650 | 8.3:-45857308 | MS.gene29921:CDS |
| CCAAAATGTGGTCGAGACTT+TGG | 0.387611 | 8.3:-45855844 | MS.gene29921:CDS |
| AACTCGCAGGTTTAGGGAAT+GGG | 0.389237 | 8.3:-45855724 | MS.gene29921:CDS |
| AATGCAAGTAAGGATTATTT+GGG | 0.389997 | 8.3:-45856047 | MS.gene29921:CDS |
| TGATGCAGCAAGAGACTTGT+TGG | 0.401616 | 8.3:-45855424 | MS.gene29921:CDS |
| GATGATTTGTCTGTATCAAT+GGG | 0.407748 | 8.3:-45857162 | MS.gene29921:CDS |
| CTTTGAGTTCAATGCAAGTA+AGG | 0.409663 | 8.3:-45856057 | MS.gene29921:CDS |
| CCTTCTTCTAGTTCTGGCTC+TGG | 0.429903 | 8.3:+45855873 | None:intergenic |
| GGTCGAGACTTTGGATTTCA+AGG | 0.447344 | 8.3:-45855835 | MS.gene29921:CDS |
| CAGTTATTTGGAGAAATTGA+AGG | 0.448720 | 8.3:-45855535 | MS.gene29921:CDS |
| GTTGAAGCAGTTAAGTTAGC+TGG | 0.452845 | 8.3:+45855900 | None:intergenic |
| GTTAGAATTGGAAAGGCTAA+TGG | 0.457549 | 8.3:-45855475 | MS.gene29921:CDS |
| CATTTAATTTCAATTTGACT+TGG | 0.466420 | 8.3:-45855593 | MS.gene29921:CDS |
| GGTAGCTCTAGAGTTAGAAT+TGG | 0.468868 | 8.3:-45855487 | MS.gene29921:CDS |
| ATGGATTTGGATGCTGAACT+AGG | 0.469976 | 8.3:-45855381 | MS.gene29921:CDS |
| TCTGAGCCTTTCATCCTTGG+CGG | 0.492833 | 8.3:-45857276 | MS.gene29921:CDS |
| GAGCTTCTTGATCCTGAAAG+AGG | 0.499293 | 8.3:-45856002 | MS.gene29921:CDS |
| TCATCCTTGGCGGCTATCCA+TGG | 0.501161 | 8.3:-45857266 | MS.gene29921:intron |
| AAACTCGCAGGTTTAGGGAA+TGG | 0.505546 | 8.3:-45855725 | MS.gene29921:CDS |
| TTATTTCCTTAAGACAAGAA+AGG | 0.506188 | 8.3:-45855676 | MS.gene29921:CDS |
| GTCGAGACTTTGGATTTCAA+GGG | 0.521430 | 8.3:-45855834 | MS.gene29921:CDS |
| TTTGGAGAAATTGAAGGAGG+CGG | 0.535745 | 8.3:-45855529 | MS.gene29921:CDS |
| AGCTTCTTGATCCTGAAAGA+GGG | 0.537917 | 8.3:-45856001 | MS.gene29921:CDS |
| GCCAGCAAGACACTTACAAA+AGG | 0.544024 | 8.3:-45857023 | MS.gene29921:intron |
| GAACATACTTCCTCTAGTAA+TGG | 0.544728 | 8.3:+45855783 | None:intergenic |
| TGAAAGAGGGTTTATTGTGA+AGG | 0.555016 | 8.3:-45855988 | MS.gene29921:CDS |
| TCCAAAGCAGATTGAACACA+AGG | 0.565327 | 8.3:+45855564 | None:intergenic |
| GGATAGCCGCCAAGGATGAA+AGG | 0.566403 | 8.3:+45857270 | None:intergenic |
| TCCTGACACAAAATAAGCGA+AGG | 0.566723 | 8.3:+45855753 | None:intergenic |
| CCAGAGCCAGAACTAGAAGA+AGG | 0.572474 | 8.3:-45855873 | MS.gene29921:CDS |
| CGAGCGATGAGAAGTTCACA+TGG | 0.574026 | 8.3:-45857344 | MS.gene29921:CDS |
| ACTTTGGATTTCAAGGGTGT+AGG | 0.575922 | 8.3:-45855828 | MS.gene29921:CDS |
| GGCCTTTATTCCATTACTAG+AGG | 0.578841 | 8.3:-45855793 | MS.gene29921:CDS |
| TACACTGCTAATATGTTTCA+GGG | 0.579049 | 8.3:-45857095 | MS.gene29921:CDS |
| CTGCTAATATGTTTCAGGGA+TGG | 0.579409 | 8.3:-45857091 | MS.gene29921:CDS |
| CTCTAGAGTTAGAATTGGAA+AGG | 0.585395 | 8.3:-45855482 | MS.gene29921:CDS |
| CATACCATGGATAGCCGCCA+AGG | 0.606316 | 8.3:+45857262 | None:intergenic |
| TGCAGCAAGAGACTTGTTGG+AGG | 0.618326 | 8.3:-45855421 | MS.gene29921:CDS |
| AGAAGGCCAACGCCAAAATG+TGG | 0.623253 | 8.3:-45855856 | MS.gene29921:CDS |
| TTATTTGGAGAAATTGAAGG+AGG | 0.644664 | 8.3:-45855532 | MS.gene29921:CDS |
| CCTGACACAAAATAAGCGAA+GGG | 0.648287 | 8.3:+45855754 | None:intergenic |
| GGGTGTAGGACAAATAGAGA+AGG | 0.651657 | 8.3:-45855814 | MS.gene29921:CDS |
| CTAACTTAACTGCTTCAACA+AGG | 0.670044 | 8.3:-45855896 | MS.gene29921:CDS |
| TGATTTGTCTGTATCAATGG+GGG | 0.682465 | 8.3:-45857160 | MS.gene29921:CDS |
| ATGATTTGTCTGTATCAATG+GGG | 0.692803 | 8.3:-45857161 | MS.gene29921:CDS |
| ATAAAGAACTCTGCCCAAAG+CGG | 0.731605 | 8.3:+45855695 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAGGAAAAATAAGAATT+TGG | - | chr8.3:45856492-45856511 | MS.gene29921:intron | 15.0% |
| !! | TAAGAATCTTCAAAAAATTT+GGG | - | chr8.3:45857097-45857116 | MS.gene29921:CDS | 15.0% |
| !! | TATTTGGTTAAAATAAGTTT+AGG | + | chr8.3:45855911-45855930 | None:intergenic | 15.0% |
| !!! | TATAACTTTTTTTGGTTATT+TGG | + | chr8.3:45855927-45855946 | None:intergenic | 15.0% |
| !! | AATACAATAATGAGCAAAAA+TGG | + | chr8.3:45856150-45856169 | None:intergenic | 20.0% |
| !! | ACATTAGAAGTTTAAAAAGT+CGG | + | chr8.3:45856268-45856287 | None:intergenic | 20.0% |
| !! | CAAGTCAAATTGAAATTAAA+TGG | + | chr8.3:45857134-45857153 | None:intergenic | 20.0% |
| !! | GTAAGAATCTTCAAAAAATT+TGG | - | chr8.3:45857096-45857115 | MS.gene29921:CDS | 20.0% |
| !!! | CATTTAATTTCAATTTGACT+TGG | - | chr8.3:45857132-45857151 | MS.gene29921:CDS | 20.0% |
| !!! | CTAAGTTATTTTAGATTAGT+CGG | - | chr8.3:45855776-45855795 | MS.gene29921:CDS | 20.0% |
| ! | AATGCAAGTAAGGATTATTT+GGG | - | chr8.3:45856678-45856697 | MS.gene29921:intron | 25.0% |
| ! | TGTTAGTAATTTCATAGAGT+TGG | - | chr8.3:45856586-45856605 | MS.gene29921:intron | 25.0% |
| ! | TTATTTCCTTAAGACAAGAA+AGG | - | chr8.3:45857049-45857068 | MS.gene29921:CDS | 25.0% |
| ! | TTCATTCAAATATCATACCA+TGG | + | chr8.3:45855479-45855498 | None:intergenic | 25.0% |
| ! | TTCCAACTCTTTCTAATATT+GGG | + | chr8.3:45855986-45856005 | None:intergenic | 25.0% |
| ! | TTGAATATTAGAGTACAACT+AGG | - | chr8.3:45855753-45855772 | MS.gene29921:CDS | 25.0% |
| ! | TTTCCAACTCTTTCTAATAT+TGG | + | chr8.3:45855987-45856006 | None:intergenic | 25.0% |
| ! | TTTGGAAAAGAACAGTTATT+TGG | - | chr8.3:45857178-45857197 | MS.gene29921:intron | 25.0% |
| !!! | CTTTTTATTTTTCAGTCTCA+AGG | - | chr8.3:45856416-45856435 | MS.gene29921:intron | 25.0% |
| CAATAGTTTCGTATCATATG+AGG | + | chr8.3:45855513-45855532 | None:intergenic | 30.0% | |
| CAATGCAAGTAAGGATTATT+TGG | - | chr8.3:45856677-45856696 | MS.gene29921:intron | 30.0% | |
| CAGTTATTTGGAGAAATTGA+AGG | - | chr8.3:45857190-45857209 | MS.gene29921:intron | 30.0% | |
| GAATCTTCAAAAAATTTGGG+AGG | - | chr8.3:45857100-45857119 | MS.gene29921:CDS | 30.0% | |
| GTATTTGTTCTATGTTGCAA+AGG | + | chr8.3:45856375-45856394 | None:intergenic | 30.0% | |
| TAAACAAACAGCAAAAGAGA+CGG | + | chr8.3:45856539-45856558 | None:intergenic | 30.0% | |
| TACACTGCTAATATGTTTCA+GGG | - | chr8.3:45855630-45855649 | MS.gene29921:CDS | 30.0% | |
| TCTCTCACTTGAATAAGATT+AGG | - | chr8.3:45855417-45855436 | MS.gene29921:CDS | 30.0% | |
| TTATTTGGAGAAATTGAAGG+AGG | - | chr8.3:45857193-45857212 | MS.gene29921:intron | 30.0% | |
| TTCCCAATATTAGAAAGAGT+TGG | - | chr8.3:45855981-45856000 | MS.gene29921:CDS | 30.0% | |
| TTGAACTCAAAGTTAGATTC+TGG | + | chr8.3:45856660-45856679 | None:intergenic | 30.0% | |
| ! | AAAAAGTCGGAATTTAGAGT+TGG | + | chr8.3:45856255-45856274 | None:intergenic | 30.0% |
| ! | AGTAAGGATTATTTGGGTTT+CGG | - | chr8.3:45856684-45856703 | MS.gene29921:intron | 30.0% |
| ! | AGTTAGATTCTGGAAAATTC+AGG | + | chr8.3:45856650-45856669 | None:intergenic | 30.0% |
| ! | ATGATTTGTCTGTATCAATG+GGG | - | chr8.3:45855564-45855583 | MS.gene29921:CDS | 30.0% |
| ! | GATGATTTGTCTGTATCAAT+GGG | - | chr8.3:45855563-45855582 | MS.gene29921:CDS | 30.0% |
| ! | TTCCTCTAGTAATGGAATAA+AGG | + | chr8.3:45856937-45856956 | None:intergenic | 30.0% |
| !! | AGATTTTGAAGAGATGGATT+TGG | - | chr8.3:45857331-45857350 | MS.gene29921:CDS | 30.0% |
| !! | TCAAGAAGTGTCAATTTAGT+TGG | + | chr8.3:45856625-45856644 | None:intergenic | 30.0% |
| !!! | TTGTGTTTTGACTTTGCTTT+AGG | - | chr8.3:45855541-45855560 | MS.gene29921:CDS | 30.0% |
| AGAAAAAAACTCGCAGGTTT+AGG | - | chr8.3:45856994-45857013 | MS.gene29921:intron | 35.0% | |
| AGGTCAAAAATCAAGACTCA+GGG | + | chr8.3:45855875-45855894 | None:intergenic | 35.0% | |
| CTAACTTAACTGCTTCAACA+AGG | - | chr8.3:45856829-45856848 | MS.gene29921:intron | 35.0% | |
| GAAAAAAACTCGCAGGTTTA+GGG | - | chr8.3:45856995-45857014 | MS.gene29921:intron | 35.0% | |
| GAACATACTTCCTCTAGTAA+TGG | + | chr8.3:45856945-45856964 | None:intergenic | 35.0% | |
| GGAAAAACTAAGGGATAATG+TGG | - | chr8.3:45857217-45857236 | MS.gene29921:intron | 35.0% | |
| GTACACTGCTAATATGTTTC+AGG | - | chr8.3:45855629-45855648 | MS.gene29921:CDS | 35.0% | |
| GTTAGAATTGGAAAGGCTAA+TGG | - | chr8.3:45857250-45857269 | MS.gene29921:intron | 35.0% | |
| TAGGTCAAAAATCAAGACTC+AGG | + | chr8.3:45855876-45855895 | None:intergenic | 35.0% | |
| TGAAAGAGGGTTTATTGTGA+AGG | - | chr8.3:45856737-45856756 | MS.gene29921:intron | 35.0% | |
| TGCGCTCAACAAAATCATAA+AGG | - | chr8.3:45856476-45856495 | MS.gene29921:intron | 35.0% | |
| TGTATTGTGCGTGAAATTTG+TGG | - | chr8.3:45856164-45856183 | MS.gene29921:intron | 35.0% | |
| TTCACAATAAACCCTCTTTC+AGG | + | chr8.3:45856738-45856757 | None:intergenic | 35.0% | |
| ! | ATTTTGTTGAGCGCAAAACA+TGG | + | chr8.3:45856471-45856490 | None:intergenic | 35.0% |
| ! | CTTTGAGTTCAATGCAAGTA+AGG | - | chr8.3:45856668-45856687 | MS.gene29921:intron | 35.0% |
| ! | GGATGATTTGTCTGTATCAA+TGG | - | chr8.3:45855562-45855581 | MS.gene29921:CDS | 35.0% |
| ! | TGATTTGTCTGTATCAATGG+GGG | - | chr8.3:45855565-45855584 | MS.gene29921:CDS | 35.0% |
| !! | CTCTAGAGTTAGAATTGGAA+AGG | - | chr8.3:45857243-45857262 | MS.gene29921:intron | 35.0% |
| !! | TAACTATACCTCTGAAGCAT+GGG | - | chr8.3:45856054-45856073 | MS.gene29921:CDS | 35.0% |
| !! | TTAACTATACCTCTGAAGCA+TGG | - | chr8.3:45856053-45856072 | MS.gene29921:CDS | 35.0% |
| AAACAGCAAAAGAGACGGAT+TGG | + | chr8.3:45856534-45856553 | None:intergenic | 40.0% | |
| AAACGGTGAAGTACATGTAC+CGG | - | chr8.3:45856082-45856101 | MS.gene29921:intron | 40.0% | |
| AGCTTCTTGATCCTGAAAGA+GGG | - | chr8.3:45856724-45856743 | MS.gene29921:intron | 40.0% | |
| ATAAAGAACTCTGCCCAAAG+CGG | + | chr8.3:45857033-45857052 | None:intergenic | 40.0% | |
| ATGAGTCACAGTAAGATTGC+AGG | - | chr8.3:45855811-45855830 | MS.gene29921:CDS | 40.0% | |
| ATGGATTTGGATGCTGAACT+AGG | - | chr8.3:45857344-45857363 | MS.gene29921:CDS | 40.0% | |
| CCTGACACAAAATAAGCGAA+GGG | + | chr8.3:45856974-45856993 | None:intergenic | 40.0% | |
| CTCTCACCTTTCTTGTCTTA+AGG | + | chr8.3:45857058-45857077 | None:intergenic | 40.0% | |
| CTGCTAATATGTTTCAGGGA+TGG | - | chr8.3:45855634-45855653 | MS.gene29921:CDS | 40.0% | |
| GGCCTTTATTCCATTACTAG+AGG | - | chr8.3:45856932-45856951 | MS.gene29921:intron | 40.0% | |
| GTCGAGACTTTGGATTTCAA+GGG | - | chr8.3:45856891-45856910 | MS.gene29921:intron | 40.0% | |
| GTGAAGGATGCTTGTATTGT+TGG | - | chr8.3:45856753-45856772 | MS.gene29921:intron | 40.0% | |
| GTTGAAGCAGTTAAGTTAGC+TGG | + | chr8.3:45856828-45856847 | None:intergenic | 40.0% | |
| TCCAAAGCAGATTGAACACA+AGG | + | chr8.3:45857164-45857183 | None:intergenic | 40.0% | |
| TCCTGACACAAAATAAGCGA+AGG | + | chr8.3:45856975-45856994 | None:intergenic | 40.0% | |
| TTTGGAGAAATTGAAGGAGG+CGG | - | chr8.3:45857196-45857215 | MS.gene29921:intron | 40.0% | |
| ! | ACCTTTTGTAAGTGTCTTGC+TGG | + | chr8.3:45855706-45855725 | None:intergenic | 40.0% |
| ! | ACTTTGGATTTCAAGGGTGT+AGG | - | chr8.3:45856897-45856916 | MS.gene29921:intron | 40.0% |
| ! | CTGAAGCATGGGTTGTAAAA+CGG | - | chr8.3:45856065-45856084 | MS.gene29921:CDS | 40.0% |
| ! | GTTTAGGTTCAGAGTTCACT+AGG | + | chr8.3:45855895-45855914 | None:intergenic | 40.0% |
| ! | TTTTACAACCCATGCTTCAG+AGG | + | chr8.3:45856065-45856084 | None:intergenic | 40.0% |
| !! | GGCAGAAGATTTTGAAGAGA+TGG | - | chr8.3:45857325-45857344 | MS.gene29921:CDS | 40.0% |
| !! | GGTAGCTCTAGAGTTAGAAT+TGG | - | chr8.3:45857238-45857257 | MS.gene29921:intron | 40.0% |
| AAACTCGCAGGTTTAGGGAA+TGG | - | chr8.3:45857000-45857019 | MS.gene29921:intron | 45.0% | |
| AACGGTGAAGTACATGTACC+GGG | - | chr8.3:45856083-45856102 | MS.gene29921:intron | 45.0% | |
| AACTCGCAGGTTTAGGGAAT+GGG | - | chr8.3:45857001-45857020 | MS.gene29921:intron | 45.0% | |
| CCAAAATGTGGTCGAGACTT+TGG | - | chr8.3:45856881-45856900 | MS.gene29921:intron | 45.0% | |
| GAAGGAGGCGGAAAAACTAA+GGG | - | chr8.3:45857208-45857227 | MS.gene29921:intron | 45.0% | |
| GAGCTTCTTGATCCTGAAAG+AGG | - | chr8.3:45856723-45856742 | MS.gene29921:intron | 45.0% | |
| GCCAGCAAGACACTTACAAA+AGG | - | chr8.3:45855702-45855721 | MS.gene29921:CDS | 45.0% | |
| GCCTTGTGTTCAATCTGCTT+TGG | - | chr8.3:45857160-45857179 | MS.gene29921:CDS | 45.0% | |
| GGGTGTAGGACAAATAGAGA+AGG | - | chr8.3:45856911-45856930 | MS.gene29921:intron | 45.0% | |
| GGTCGAGACTTTGGATTTCA+AGG | - | chr8.3:45856890-45856909 | MS.gene29921:intron | 45.0% | |
| GTCAGGAGAAAAAAACTCGC+AGG | - | chr8.3:45856988-45857007 | MS.gene29921:intron | 45.0% | |
| TACTCTGAGCCTTTCATCCT+TGG | - | chr8.3:45855446-45855465 | MS.gene29921:CDS | 45.0% | |
| TGAAGGAGGCGGAAAAACTA+AGG | - | chr8.3:45857207-45857226 | MS.gene29921:intron | 45.0% | |
| TGATGCAGCAAGAGACTTGT+TGG | - | chr8.3:45857301-45857320 | MS.gene29921:CDS | 45.0% | |
| ! | CCAAAGTCTCGACCACATTT+TGG | + | chr8.3:45856884-45856903 | None:intergenic | 45.0% |
| !!! | CCCTTCGCTTATTTTGTGTC+AGG | - | chr8.3:45856971-45856990 | MS.gene29921:intron | 45.0% |
| !!! | TTTAATTTTATAACTTTTTT+TGG | + | chr8.3:45855935-45855954 | None:intergenic | 5.0% |
| AGAAGGCCAACGCCAAAATG+TGG | - | chr8.3:45856869-45856888 | MS.gene29921:intron | 50.0% | |
| CCAGAGCCAGAACTAGAAGA+AGG | - | chr8.3:45856852-45856871 | MS.gene29921:intron | 50.0% | |
| CGAGCGATGAGAAGTTCACA+TGG | - | chr8.3:45855381-45855400 | MS.gene29921:CDS | 50.0% | |
| TCTGAGCCTTTCATCCTTGG+CGG | - | chr8.3:45855449-45855468 | MS.gene29921:CDS | 50.0% | |
| TGTCTGTATCAATGGGGGAC+AGG | - | chr8.3:45855570-45855589 | MS.gene29921:CDS | 50.0% | |
| !! | CCTTCTTCTAGTTCTGGCTC+TGG | + | chr8.3:45856855-45856874 | None:intergenic | 50.0% |
| !! | CGTTGGCCTTCTTCTAGTTC+TGG | + | chr8.3:45856861-45856880 | None:intergenic | 50.0% |
| !! | TCTCGACCACATTTTGGCGT+TGG | + | chr8.3:45856878-45856897 | None:intergenic | 50.0% |
| !! | TGCAGCAAGAGACTTGTTGG+AGG | - | chr8.3:45857304-45857323 | MS.gene29921:CDS | 50.0% |
| CATACCATGGATAGCCGCCA+AGG | + | chr8.3:45855466-45855485 | None:intergenic | 55.0% | |
| GAATGGGCGTTCACCGCTTT+GGG | - | chr8.3:45857017-45857036 | MS.gene29921:intron | 55.0% | |
| GGATAGCCGCCAAGGATGAA+AGG | + | chr8.3:45855458-45855477 | None:intergenic | 55.0% | |
| TACCGTACGCGTACCCAGTA+CGG | + | chr8.3:45856116-45856135 | None:intergenic | 55.0% | |
| TCATCCTTGGCGGCTATCCA+TGG | - | chr8.3:45855459-45855478 | MS.gene29921:CDS | 55.0% | |
| ACCCAGTACGGGTGTGTACC+CGG | + | chr8.3:45856104-45856123 | None:intergenic | 60.0% | |
| ACCCGTACTGGGTACGCGTA+CGG | - | chr8.3:45856111-45856130 | MS.gene29921:intron | 60.0% | |
| ACCGGGTACACACCCGTACT+GGG | - | chr8.3:45856100-45856119 | MS.gene29921:intron | 60.0% | |
| ACCGTACGCGTACCCAGTAC+GGG | + | chr8.3:45856115-45856134 | None:intergenic | 60.0% | |
| GGAATGGGCGTTCACCGCTT+TGG | - | chr8.3:45857016-45857035 | MS.gene29921:intron | 60.0% | |
| GGGTACGCGTACGGTACTTG+TGG | - | chr8.3:45856120-45856139 | MS.gene29921:intron | 60.0% | |
| TACCGGGTACACACCCGTAC+TGG | - | chr8.3:45856099-45856118 | MS.gene29921:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 45855365 | 45857382 | 45855365 | ID=MS.gene29921 |
| chr8.3 | mRNA | 45855365 | 45857382 | 45855365 | ID=MS.gene29921.t1;Parent=MS.gene29921 |
| chr8.3 | exon | 45857267 | 45857382 | 45857267 | ID=MS.gene29921.t1.exon1;Parent=MS.gene29921.t1 |
| chr8.3 | CDS | 45857267 | 45857382 | 45857267 | ID=cds.MS.gene29921.t1;Parent=MS.gene29921.t1 |
| chr8.3 | exon | 45857024 | 45857184 | 45857024 | ID=MS.gene29921.t1.exon2;Parent=MS.gene29921.t1 |
| chr8.3 | CDS | 45857024 | 45857184 | 45857024 | ID=cds.MS.gene29921.t1;Parent=MS.gene29921.t1 |
| chr8.3 | exon | 45855365 | 45856086 | 45855365 | ID=MS.gene29921.t1.exon3;Parent=MS.gene29921.t1 |
| chr8.3 | CDS | 45855365 | 45856086 | 45855365 | ID=cds.MS.gene29921.t1;Parent=MS.gene29921.t1 |
| Gene Sequence |
| Protein sequence |
