AlfalfaGEDB | MS.gene96992

M. sativa cultivar XinJiangDaYe / MS.gene96992

KEGG: -
GO: GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0033036 GO:0042886 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0071702 GO:0071705
PFAM: PF00790.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96992.t1	XP_013449904.1	98.1	162	3	0	1	162	1	162	1.90E-83	318.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96992.t1	Q9LNC6	71.6	162	46	0	1	162	1	162	2.3e-61	236.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96992.t1	A0A072U2J4	98.1	162	3	0	1	162	1	162	1.4e-83	318.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene96992	0.82388	1.09E-53	-1.69E-46
MS.gene049283	MS.gene96992	0.84552	3.86E-59	-1.69E-46
MS.gene049419	MS.gene96992	-0.81012	1.35E-50	-1.69E-46
MS.gene049499	MS.gene96992	0.827299	1.69E-54	-1.69E-46
MS.gene049983	MS.gene96992	0.842364	2.71E-58	-1.69E-46
MS.gene050105	MS.gene96992	0.803136	4.03E-49	-1.69E-46
MS.gene050273	MS.gene96992	0.80238	5.77E-49	-1.69E-46
MS.gene050646	MS.gene96992	0.808887	2.48E-50	-1.69E-46
MS.gene050654	MS.gene96992	0.802956	4.39E-49	-1.69E-46
MS.gene050945	MS.gene96992	0.80208	6.65E-49	-1.69E-46
MS.gene051198	MS.gene96992	0.825052	5.78E-54	-1.69E-46
MS.gene051332	MS.gene96992	0.836481	9.12E-57	-1.69E-46
MS.gene051628	MS.gene96992	0.816908	4.34E-52	-1.69E-46
MS.gene052037	MS.gene96992	0.821599	3.71E-53	-1.69E-46
MS.gene052108	MS.gene96992	0.801373	9.28E-49	-1.69E-46
MS.gene052239	MS.gene96992	-0.835012	2.15E-56	-1.69E-46
MS.gene052463	MS.gene96992	0.802911	4.48E-49	-1.69E-46
MS.gene052954	MS.gene96992	0.808033	3.78E-50	-1.69E-46
MS.gene05308	MS.gene96992	0.827905	1.21E-54	-1.69E-46
MS.gene053272	MS.gene96992	-0.801264	9.77E-49	-1.69E-46
MS.gene053931	MS.gene96992	0.815674	8.19E-52	-1.69E-46
MS.gene053963	MS.gene96992	0.80841	3.14E-50	-1.69E-46
MS.gene054479	MS.gene96992	0.823987	1.03E-53	-1.69E-46
MS.gene054576	MS.gene96992	0.810444	1.15E-50	-1.69E-46
MS.gene054651	MS.gene96992	0.819819	9.51E-53	-1.69E-46
MS.gene055228	MS.gene96992	0.803667	3.13E-49	-1.69E-46
MS.gene056045	MS.gene96992	0.835721	1.42E-56	-1.69E-46
MS.gene056272	MS.gene96992	0.803937	2.75E-49	-1.69E-46
MS.gene056776	MS.gene96992	0.813984	1.94E-51	-1.69E-46
MS.gene05686	MS.gene96992	0.831003	2.13E-55	-1.69E-46
MS.gene056973	MS.gene96992	0.868192	7.84E-66	-1.69E-46
MS.gene057208	MS.gene96992	0.821262	4.43E-53	-1.69E-46
MS.gene057598	MS.gene96992	0.825208	5.31E-54	-1.69E-46
MS.gene057855	MS.gene96992	0.800868	1.18E-48	-1.69E-46
MS.gene057919	MS.gene96992	0.816323	5.87E-52	-1.69E-46
MS.gene058010	MS.gene96992	0.801108	1.05E-48	-1.69E-46
MS.gene058159	MS.gene96992	0.802931	4.44E-49	-1.69E-46
MS.gene058223	MS.gene96992	-0.813225	2.85E-51	-1.69E-46
MS.gene058279	MS.gene96992	0.835016	2.14E-56	-1.69E-46
MS.gene058520	MS.gene96992	0.800366	1.49E-48	-1.69E-46
MS.gene058781	MS.gene96992	0.826631	2.44E-54	-1.69E-46
MS.gene058979	MS.gene96992	0.804945	1.69E-49	-1.69E-46
MS.gene059439	MS.gene96992	0.814586	1.43E-51	-1.69E-46
MS.gene05952	MS.gene96992	0.808118	3.62E-50	-1.69E-46
MS.gene05985	MS.gene96992	-0.830678	2.57E-55	-1.69E-46
MS.gene060365	MS.gene96992	0.802263	6.10E-49	-1.69E-46
MS.gene060527	MS.gene96992	0.818247	2.17E-52	-1.69E-46
MS.gene060777	MS.gene96992	0.824908	6.25E-54	-1.69E-46
MS.gene060933	MS.gene96992	0.833869	4.16E-56	-1.69E-46
MS.gene060935	MS.gene96992	0.849879	2.44E-60	-1.69E-46
MS.gene060937	MS.gene96992	0.861814	7.87E-64	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96992.t1	MTR_7g097010	98.148	162	3	0	1	162	1	162	2.28e-118	341
MS.gene96992.t1	MTR_5g007480	46.667	165	80	2	5	161	8	172	1.03e-42	147
MS.gene96992.t1	MTR_8g099600	95.385	65	3	0	57	121	1	65	1.35e-39	129
MS.gene96992.t1	MTR_4g077940	44.048	168	85	3	3	161	6	173	1.13e-35	129
MS.gene96992.t1	MTR_4g077940	44.048	168	85	3	3	161	33	200	1.39e-35	129
MS.gene96992.t1	MTR_7g072310	34.579	107	68	2	43	148	15	120	8.09e-16	74.7
MS.gene96992.t1	MTR_3g110108	34.862	109	69	2	41	148	5	112	9.86e-16	74.3
MS.gene96992.t1	MTR_3g110108	34.862	109	69	2	41	148	5	112	5.58e-15	72.4
MS.gene96992.t1	MTR_1g026840	34.234	111	71	2	39	148	3	112	8.94e-15	71.6
MS.gene96992.t1	MTR_3g088805	32.407	108	71	2	42	148	9	115	8.09e-14	68.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene96992.t1	AT1G06210	71.605	162	46	0	1	162	1	162	7.12e-83	246
MS.gene96992.t1	AT1G06210	71.605	162	46	0	1	162	1	162	5.22e-82	248
MS.gene96992.t1	AT5G16880	47.879	165	78	2	5	161	8	172	4.82e-50	163
MS.gene96992.t1	AT5G16880	47.879	165	78	2	5	161	8	172	1.36e-48	162
MS.gene96992.t1	AT5G16880	47.879	165	78	2	5	161	8	172	1.36e-48	162
MS.gene96992.t1	AT5G16880	47.879	165	78	2	5	161	8	172	1.36e-48	162
MS.gene96992.t1	AT4G32760	37.615	109	66	2	41	148	5	112	1.25e-17	79.7
MS.gene96992.t1	AT5G01760	39.815	108	63	2	42	148	26	132	1.80e-17	79.3
MS.gene96992.t1	AT4G32760	37.615	109	66	2	41	148	5	112	4.10e-17	78.6
MS.gene96992.t1	AT4G32760	37.615	109	66	2	41	148	5	112	4.18e-17	78.6
MS.gene96992.t1	AT3G08790	33.945	109	70	2	41	148	5	112	1.93e-13	67.8
MS.gene96992.t1	AT1G21380	31.481	108	72	2	42	148	9	115	2.13e-12	64.7
MS.gene96992.t1	AT2G38410	32.710	107	70	2	43	148	13	118	9.11e-12	63.2
MS.gene96992.t1	AT1G76970	30.556	108	73	2	42	148	9	115	4.00e-11	60.8
MS.gene96992.t1	AT1G76970	30.556	108	73	2	42	148	9	115	4.00e-11	60.8
MS.gene96992.t1	AT1G76970	30.556	108	73	2	42	148	9	115	4.00e-11	60.8
MS.gene96992.t1	AT1G76970	30.556	108	73	2	42	148	9	115	4.00e-11	60.8

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTATCTGAACCAAAT+TGG	0.209591	7.4:+12623679	MS.gene96992:CDS
TGTTGCTTCGTCGACCATTT+TGG	0.217475	7.4:-12623652	None:intergenic
TAAACTCTTCGGCGTTAATA+AGG	0.243065	7.4:-12623725	None:intergenic
CTGAGATTCATGCCCCAATT+TGG	0.268510	7.4:-12623693	None:intergenic
AACATTATCTGAACCAAATT+GGG	0.287314	7.4:+12623680	MS.gene96992:CDS
TGAGAAGGTTCTTGATGATA+TGG	0.294087	7.4:+12623878	MS.gene96992:CDS
TCGGCTCATGTTGGCGCTGC+TGG	0.319679	7.4:-12623580	None:intergenic
TGGATTCAGGTGTTGGTGTT+TGG	0.344888	7.4:-12623632	None:intergenic
GAAACATAGTCTTGATTTGT+TGG	0.357292	7.4:+12623809	MS.gene96992:CDS
TAGGGCTTGGGGTGAGTCTC+AGG	0.372522	7.4:+12623962	MS.gene96992:CDS
TTCAGAACCGTTAAACTCTT+CGG	0.431789	7.4:-12623736	None:intergenic
AGACTATGTTTCTGAACAAC+AGG	0.445577	7.4:-12623798	None:intergenic
ACCAACACCTGAATCCAAAA+TGG	0.446625	7.4:+12623638	MS.gene96992:CDS
AGGTTCTTGATGATATGGTT+AGG	0.452679	7.4:+12623883	MS.gene96992:CDS
ATTAACGCCGAAGAGTTTAA+CGG	0.462205	7.4:+12623729	MS.gene96992:CDS
ATATAAGTCTGACTGAAAAC+AGG	0.472919	7.4:-12623999	None:intergenic
AAATCAAAGCTAGCTCAATT+CGG	0.484230	7.4:+12623543	MS.gene96992:CDS
GAGAATAATCAAGGTGATGG+TGG	0.486256	7.4:+12623912	MS.gene96992:CDS
TTCTGAAATTGCTTCTGAGA+AGG	0.500375	7.4:+12623863	MS.gene96992:CDS
TTTCAGTTGATTAGGGCTTG+GGG	0.512504	7.4:+12623951	MS.gene96992:CDS
AGGTTGATTGAGAATAATCA+AGG	0.513639	7.4:+12623903	MS.gene96992:CDS
AACAAAGCAAGAAGGAAGGA+AGG	0.515027	7.4:-12624039	None:intergenic
AGCTAGCTTTGATTTGTCCA+TGG	0.517070	7.4:-12623535	None:intergenic
GTGATGGTGGGAATAGAAGA+AGG	0.535548	7.4:+12623925	MS.gene96992:CDS
AGAATAATCAAGGTGATGGT+GGG	0.549576	7.4:+12623913	MS.gene96992:CDS
AATGGTCGACGAAGCAACAT+CGG	0.555458	7.4:+12623656	MS.gene96992:CDS
AGCTAGCTCAATTCGGCGAG+AGG	0.587672	7.4:+12623550	MS.gene96992:CDS
TGATGGTGGGAATAGAAGAA+GGG	0.592961	7.4:+12623926	MS.gene96992:CDS
ATTGAGAATAATCAAGGTGA+TGG	0.608010	7.4:+12623909	MS.gene96992:CDS
GCTTACGATTCGGCTCATGT+TGG	0.617537	7.4:-12623589	None:intergenic
AACATGAGCCGAATCGTAAG+CGG	0.628012	7.4:+12623591	MS.gene96992:CDS
ACATTATCTGAACCAAATTG+GGG	0.646003	7.4:+12623681	MS.gene96992:CDS
GGCATGAATCTCAGAATCTG+CGG	0.695779	7.4:+12623702	MS.gene96992:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAACATTATCTGAACCAAAT+TGG	+	chr7.4:12623679-12623698	MS.gene96992:CDS	25.0%
!	AACATTATCTGAACCAAATT+GGG	+	chr7.4:12623680-12623699	MS.gene96992:CDS	25.0%
	AAATCAAAGCTAGCTCAATT+CGG	+	chr7.4:12623543-12623562	MS.gene96992:CDS	30.0%
	ACATTATCTGAACCAAATTG+GGG	+	chr7.4:12623681-12623700	MS.gene96992:CDS	30.0%
	ATATAAGTCTGACTGAAAAC+AGG	-	chr7.4:12624002-12624021	None:intergenic	30.0%
	ATTGAGAATAATCAAGGTGA+TGG	+	chr7.4:12623909-12623928	MS.gene96992:CDS	30.0%
!	AGGTTGATTGAGAATAATCA+AGG	+	chr7.4:12623903-12623922	MS.gene96992:CDS	30.0%
!	TGTTTTCAGTCAGACTTATA+TGG	+	chr7.4:12624001-12624020	MS.gene96992:CDS	30.0%
!!	GAAACATAGTCTTGATTTGT+TGG	+	chr7.4:12623809-12623828	MS.gene96992:CDS	30.0%
	ATTAACGCCGAAGAGTTTAA+CGG	+	chr7.4:12623729-12623748	MS.gene96992:CDS	35.0%
	GAAAAACAAAGCAAGAAGGA+AGG	-	chr7.4:12624046-12624065	None:intergenic	35.0%
	GTCTGAAAAACAAAGCAAGA+AGG	-	chr7.4:12624050-12624069	None:intergenic	35.0%
	TAAACTCTTCGGCGTTAATA+AGG	-	chr7.4:12623728-12623747	None:intergenic	35.0%
	TTCAGAACCGTTAAACTCTT+CGG	-	chr7.4:12623739-12623758	None:intergenic	35.0%
!	AAGGGCTTTTCAGTTGATTA+GGG	+	chr7.4:12623944-12623963	MS.gene96992:CDS	35.0%
!	AGAATAATCAAGGTGATGGT+GGG	+	chr7.4:12623913-12623932	MS.gene96992:CDS	35.0%
!	AGACTATGTTTCTGAACAAC+AGG	-	chr7.4:12623801-12623820	None:intergenic	35.0%
!	AGGTTCTTGATGATATGGTT+AGG	+	chr7.4:12623883-12623902	MS.gene96992:CDS	35.0%
!	TGAGAAGGTTCTTGATGATA+TGG	+	chr7.4:12623878-12623897	MS.gene96992:CDS	35.0%
!	TTTTCAGTTGATTAGGGCTT+GGG	+	chr7.4:12623950-12623969	MS.gene96992:CDS	35.0%
!!	TTCTGAAATTGCTTCTGAGA+AGG	+	chr7.4:12623863-12623882	MS.gene96992:CDS	35.0%
	AACAAAGCAAGAAGGAAGGA+AGG	-	chr7.4:12624042-12624061	None:intergenic	40.0%
	ACCAACACCTGAATCCAAAA+TGG	+	chr7.4:12623638-12623657	MS.gene96992:CDS	40.0%
	TTTCAGTTGATTAGGGCTTG+GGG	+	chr7.4:12623951-12623970	MS.gene96992:CDS	40.0%
!	ACCATTTTGGATTCAGGTGT+TGG	-	chr7.4:12623642-12623661	None:intergenic	40.0%
!	CTTTTCAGTTGATTAGGGCT+TGG	+	chr7.4:12623949-12623968	MS.gene96992:CDS	40.0%
!	GAAGGGCTTTTCAGTTGATT+AGG	+	chr7.4:12623943-12623962	MS.gene96992:CDS	40.0%
!	GAGAATAATCAAGGTGATGG+TGG	+	chr7.4:12623912-12623931	MS.gene96992:CDS	40.0%
!!	TCATTTTGCCGCTTACGATT+CGG	-	chr7.4:12623602-12623621	None:intergenic	40.0%
!!	TGATGGTGGGAATAGAAGAA+GGG	+	chr7.4:12623926-12623945	MS.gene96992:CDS	40.0%
	AACATGAGCCGAATCGTAAG+CGG	+	chr7.4:12623591-12623610	MS.gene96992:CDS	45.0%
	AATGGTCGACGAAGCAACAT+CGG	+	chr7.4:12623656-12623675	MS.gene96992:CDS	45.0%
	CTGAGATTCATGCCCCAATT+TGG	-	chr7.4:12623696-12623715	None:intergenic	45.0%
	GGCATGAATCTCAGAATCTG+CGG	+	chr7.4:12623702-12623721	MS.gene96992:CDS	45.0%
!	TCGTCGACCATTTTGGATTC+AGG	-	chr7.4:12623648-12623667	None:intergenic	45.0%
!!	GTGATGGTGGGAATAGAAGA+AGG	+	chr7.4:12623925-12623944	MS.gene96992:CDS	45.0%
!!	TGGATTCAGGTGTTGGTGTT+TGG	-	chr7.4:12623635-12623654	None:intergenic	45.0%
!!	TGTTGCTTCGTCGACCATTT+TGG	-	chr7.4:12623655-12623674	None:intergenic	45.0%
!	GCTTACGATTCGGCTCATGT+TGG	-	chr7.4:12623592-12623611	None:intergenic	50.0%
	AGCTAGCTCAATTCGGCGAG+AGG	+	chr7.4:12623550-12623569	MS.gene96992:CDS	55.0%
	TAGGGCTTGGGGTGAGTCTC+AGG	+	chr7.4:12623962-12623981	MS.gene96992:CDS	60.0%
	TCGGCTCATGTTGGCGCTGC+TGG	-	chr7.4:12623583-12623602	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	12623537	12624073	12623537	ID=MS.gene96992
chr7.4	mRNA	12623537	12624073	12623537	ID=MS.gene96992.t1;Parent=MS.gene96992
chr7.4	exon	12623537	12624073	12623537	ID=MS.gene96992.t1.exon1;Parent=MS.gene96992.t1
chr7.4	CDS	12623537	12624073	12623537	ID=cds.MS.gene96992.t1;Parent=MS.gene96992.t1

Gene Sequence

>MS.gene96992

ATGGACAAATCAAAGCTAGCTCAATTCGGCGAGAGGCTCAAAACCAGCAGCGCCAACATGAGCCGAATCGTAAGCGGCAAAATGAAAGAAATTCTCCAAACACCAACACCTGAATCCAAAATGGTCGACGAAGCAACATCGGAAACATTATCTGAACCAAATTGGGGCATGAATCTCAGAATCTGCGGCCTTATTAACGCCGAAGAGTTTAACGGTTCTGAAATAGTGAAAGCTATTAAGAGAAAGATTAATCATAAGAGTCCTGTTGTTCAGAAACATAGTCTTGATTTGTTGGAAACTTGTGCTATGAATTGTGATAAAGTGTTTTCTGAAATTGCTTCTGAGAAGGTTCTTGATGATATGGTTAGGTTGATTGAGAATAATCAAGGTGATGGTGGGAATAGAAGAAGGGCTTTTCAGTTGATTAGGGCTTGGGGTGAGTCTCAGGATATTGCTTATCTTCCTGTTTTCAGTCAGACTTATATGGTCAGTTTCTTTCTTTCCTTCCTTCCTTCTTGCTTTGTTTTTCAGACATAA

Protein sequence

>MS.gene96992.t1

MDKSKLAQFGERLKTSSANMSRIVSGKMKEILQTPTPESKMVDEATSETLSEPNWGMNLRICGLINAEEFNGSEIVKAIKRKINHKSPVVQKHSLDLLETCAMNCDKVFSEIASEKVLDDMVRLIENNQGDGGNRRRAFQLIRAWGESQDIAYLPVFSQTYMVSFFLSFLPSCFVFQT