Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54555.t1 | XP_003588519.1 | 100 | 84 | 0 | 0 | 1 | 84 | 1 | 84 | 3.30E-23 | 117.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54555.t1 | Q9STN8 | 81.2 | 32 | 6 | 0 | 53 | 84 | 46 | 77 | 1.1e-08 | 60.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54555.t1 | B7FIX0 | 100.0 | 84 | 0 | 0 | 1 | 84 | 1 | 84 | 2.4e-23 | 117.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049822 | MS.gene54555 | 0.810691 | 1.02E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene54555 | 0.820514 | 6.59E-53 | -1.69E-46 |
| MS.gene050317 | MS.gene54555 | 0.832599 | 8.62E-56 | -1.69E-46 |
| MS.gene050386 | MS.gene54555 | 0.804904 | 1.73E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene54555 | 0.829207 | 5.86E-55 | -1.69E-46 |
| MS.gene051102 | MS.gene54555 | 0.814639 | 1.39E-51 | -1.69E-46 |
| MS.gene051748 | MS.gene54555 | 0.811762 | 5.95E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene54555 | 0.818879 | 1.56E-52 | -1.69E-46 |
| MS.gene052307 | MS.gene54555 | 0.819448 | 1.16E-52 | -1.69E-46 |
| MS.gene052434 | MS.gene54555 | 0.810991 | 8.75E-51 | -1.69E-46 |
| MS.gene052527 | MS.gene54555 | 0.807482 | 4.95E-50 | -1.69E-46 |
| MS.gene052609 | MS.gene54555 | 0.809766 | 1.61E-50 | -1.69E-46 |
| MS.gene053381 | MS.gene54555 | 0.808889 | 2.48E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene54555 | 0.823795 | 1.14E-53 | -1.69E-46 |
| MS.gene055313 | MS.gene54555 | 0.812276 | 4.60E-51 | -1.69E-46 |
| MS.gene055314 | MS.gene54555 | 0.810702 | 1.01E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene54555 | 0.830876 | 2.29E-55 | -1.69E-46 |
| MS.gene056432 | MS.gene54555 | 0.822937 | 1.81E-53 | -1.69E-46 |
| MS.gene056787 | MS.gene54555 | 0.811723 | 6.07E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene54555 | 0.829 | 6.57E-55 | -1.69E-46 |
| MS.gene057094 | MS.gene54555 | 0.808806 | 2.58E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene54555 | 0.815748 | 7.89E-52 | -1.69E-46 |
| MS.gene057360 | MS.gene54555 | 0.823918 | 1.07E-53 | -1.69E-46 |
| MS.gene058054 | MS.gene54555 | 0.811575 | 6.54E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene54555 | 0.812395 | 4.33E-51 | -1.69E-46 |
| MS.gene058074 | MS.gene54555 | 0.82511 | 5.61E-54 | -1.69E-46 |
| MS.gene058638 | MS.gene54555 | 0.820871 | 5.46E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene54555 | 0.833812 | 4.30E-56 | -1.69E-46 |
| MS.gene058758 | MS.gene54555 | 0.831193 | 1.92E-55 | -1.69E-46 |
| MS.gene059171 | MS.gene54555 | 0.821954 | 3.07E-53 | -1.69E-46 |
| MS.gene059713 | MS.gene54555 | 0.820633 | 6.19E-53 | -1.69E-46 |
| MS.gene059719 | MS.gene54555 | 0.802347 | 5.86E-49 | -1.69E-46 |
| MS.gene061055 | MS.gene54555 | 0.801459 | 8.92E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54555.t1 | MTR_1g008120 | 98.810 | 84 | 1 | 0 | 1 | 84 | 1 | 84 | 6.88e-55 | 173 |
| MS.gene54555.t1 | MTR_3g091510 | 75.000 | 84 | 13 | 1 | 1 | 84 | 1 | 76 | 4.45e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54555.t1 | AT3G61790 | 47.619 | 84 | 36 | 1 | 1 | 84 | 1 | 76 | 2.93e-15 | 69.3 |
| MS.gene54555.t1 | AT5G53360 | 57.377 | 61 | 12 | 3 | 29 | 87 | 16 | 64 | 2.93e-14 | 64.7 |
| MS.gene54555.t1 | AT4G27880 | 48.864 | 88 | 30 | 4 | 1 | 84 | 1 | 77 | 3.43e-14 | 66.2 |
Find 16 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATAATCATGGTGTTAT+TGG | 0.169856 | 1.1:+11510667 | MS.gene54555:CDS |
| ATAAAGCAAACCTGATGAAT+TGG | 0.365377 | 1.1:-11510766 | None:intergenic |
| AATGTATCCTCCAATTCATC+AGG | 0.424313 | 1.1:+11510756 | MS.gene54555:CDS |
| TACATTGAATTGGTACAAAC+AGG | 0.426249 | 1.1:-11510739 | None:intergenic |
| TGTTTCTTCTTCTGATGGAA+TGG | 0.427322 | 1.1:+11510549 | MS.gene54555:CDS |
| AATTGGAGGATACATTGAAT+TGG | 0.448728 | 1.1:-11510749 | None:intergenic |
| GAGTGTGTTTCTTCTTCTGA+TGG | 0.455471 | 1.1:+11510544 | MS.gene54555:CDS |
| TACAAGTGTTCATGAGTTGT+TGG | 0.494508 | 1.1:+11510711 | MS.gene54555:CDS |
| TCTTCATTGAAACCTCGTAA+TGG | 0.515536 | 1.1:+11510640 | MS.gene54555:CDS |
| AATGGAGGAAACAATAATCA+TGG | 0.519050 | 1.1:+11510658 | MS.gene54555:CDS |
| AAGCAAACCTGATGAATTGG+AGG | 0.534378 | 1.1:-11510763 | None:intergenic |
| TGATGATGTTGTTGATGATG+TGG | 0.544739 | 1.1:-11510604 | None:intergenic |
| AACTCATGAACACTTGTAGC+AGG | 0.562232 | 1.1:-11510706 | None:intergenic |
| GTGGATGAAGAATACGATGT+TGG | 0.600992 | 1.1:-11510585 | None:intergenic |
| TCATTGAAACCTCGTAATGG+AGG | 0.608179 | 1.1:+11510643 | MS.gene54555:CDS |
| TTATTGTTTCCTCCATTACG+AGG | 0.648247 | 1.1:-11510652 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAATAATCATGGTGTTAT+TGG | + | chr1.1:11510667-11510686 | MS.gene54555:CDS | 25.0% |
| AATGGAGGAAACAATAATCA+TGG | + | chr1.1:11510658-11510677 | MS.gene54555:CDS | 30.0% | |
| AATTGGAGGATACATTGAAT+TGG | - | chr1.1:11510752-11510771 | None:intergenic | 30.0% | |
| ATAAAGCAAACCTGATGAAT+TGG | - | chr1.1:11510769-11510788 | None:intergenic | 30.0% | |
| TACATTGAATTGGTACAAAC+AGG | - | chr1.1:11510742-11510761 | None:intergenic | 30.0% | |
| AATGTATCCTCCAATTCATC+AGG | + | chr1.1:11510756-11510775 | MS.gene54555:CDS | 35.0% | |
| TACAAGTGTTCATGAGTTGT+TGG | + | chr1.1:11510711-11510730 | MS.gene54555:CDS | 35.0% | |
| TCTTCATTGAAACCTCGTAA+TGG | + | chr1.1:11510640-11510659 | MS.gene54555:CDS | 35.0% | |
| TGTTTCTTCTTCTGATGGAA+TGG | + | chr1.1:11510549-11510568 | MS.gene54555:CDS | 35.0% | |
| TTATTGTTTCCTCCATTACG+AGG | - | chr1.1:11510655-11510674 | None:intergenic | 35.0% | |
| !! | TGATGATGTTGTTGATGATG+TGG | - | chr1.1:11510607-11510626 | None:intergenic | 35.0% |
| AACTCATGAACACTTGTAGC+AGG | - | chr1.1:11510709-11510728 | None:intergenic | 40.0% | |
| AAGCAAACCTGATGAATTGG+AGG | - | chr1.1:11510766-11510785 | None:intergenic | 40.0% | |
| GAGTGTGTTTCTTCTTCTGA+TGG | + | chr1.1:11510544-11510563 | MS.gene54555:CDS | 40.0% | |
| GTGGATGAAGAATACGATGT+TGG | - | chr1.1:11510588-11510607 | None:intergenic | 40.0% | |
| TCATTGAAACCTCGTAATGG+AGG | + | chr1.1:11510643-11510662 | MS.gene54555:CDS | 40.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 11510526 | 11510798 | 11510526 | ID=MS.gene54555 |
| chr1.1 | mRNA | 11510526 | 11510798 | 11510526 | ID=MS.gene54555.t1;Parent=MS.gene54555 |
| chr1.1 | exon | 11510526 | 11510798 | 11510526 | ID=MS.gene54555.t1.exon1;Parent=MS.gene54555.t1 |
| chr1.1 | CDS | 11510526 | 11510798 | 11510526 | ID=cds.MS.gene54555.t1;Parent=MS.gene54555.t1 |
| Gene Sequence |
| Protein sequence |
