AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76478


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76478.t1 MTR_8g076070 98.780 82 1 0 1 82 1 82 6.68e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76478.t1 AT2G18040 74.324 74 19 0 9 82 6 79 3.90e-36 119

Find 20 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAAGAGGATATCCTTTC+TGG 0.182475 8.1:-31371627 MS.gene76478:CDS
CGAAGCAAGATCCTCAAATT+TGG 0.289474 8.1:+31371599 None:intergenic
CTCGCGGCGGCGCTGAGGTT+AGG 0.301148 8.1:-31371767 MS.gene76478:CDS
TTCTTGATGACGTAACCTTC+TGG 0.327996 8.1:+31371687 None:intergenic
GCTAAACGCGGTGGCGATCT+TGG 0.368998 8.1:-31371555 MS.gene76478:CDS
TCGCGGCGGCGCTGAGGTTA+GGG 0.411373 8.1:-31371766 MS.gene76478:CDS
TTCTGGTAAGGCCAAATTTG+AGG 0.428366 8.1:-31371610 MS.gene76478:CDS
CAAATTTGGCCTTACCAGAA+AGG 0.448271 8.1:+31371613 None:intergenic
TATTCTTACTTCAATGAAAG+AGG 0.454286 8.1:-31371640 MS.gene76478:CDS
AGAGGATATCCTTTCTGGTA+AGG 0.533949 8.1:-31371622 MS.gene76478:CDS
AATTTGAGGATCTTGCTTCG+AGG 0.550226 8.1:-31371596 MS.gene76478:CDS
GTTCTAGAAGAAAAGCTTCG+TGG 0.556909 8.1:-31371716 MS.gene76478:CDS
TTCATCTCGCGGCGGCGCTG+AGG 0.579649 8.1:-31371772 MS.gene76478:CDS
CATTGAAGTAAGAATAGCAA+CGG 0.597353 8.1:+31371647 None:intergenic
GCGAAAATGTCTTCATCTCG+CGG 0.645162 8.1:-31371783 None:intergenic
CACATACTGATTAAACACGA+AGG 0.672969 8.1:-31371738 MS.gene76478:CDS
TGTAGCTCTGCTAAACGCGG+TGG 0.678782 8.1:-31371564 MS.gene76478:CDS
GATTGTAGCTCTGCTAAACG+CGG 0.692633 8.1:-31371567 MS.gene76478:CDS
GCTTCGTGGAAAGATCCAGA+AGG 0.706542 8.1:-31371702 MS.gene76478:CDS
AAAATGTCTTCATCTCGCGG+CGG 0.772656 8.1:-31371780 MS.gene76478:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATTCTTACTTCAATGAAAG+AGG - chr8.1:31371655-31371674 MS.gene76478:CDS 25.0%
CATTGAAGTAAGAATAGCAA+CGG + chr8.1:31371651-31371670 None:intergenic 30.0%
ATGAAAGAGGATATCCTTTC+TGG - chr8.1:31371668-31371687 MS.gene76478:CDS 35.0%
! CACATACTGATTAAACACGA+AGG - chr8.1:31371557-31371576 MS.gene76478:CDS 35.0%
AATTTGAGGATCTTGCTTCG+AGG - chr8.1:31371699-31371718 MS.gene76478:CDS 40.0%
CGAAGCAAGATCCTCAAATT+TGG + chr8.1:31371699-31371718 None:intergenic 40.0%
TTCTGGTAAGGCCAAATTTG+AGG - chr8.1:31371685-31371704 MS.gene76478:CDS 40.0%
! AGAGGATATCCTTTCTGGTA+AGG - chr8.1:31371673-31371692 MS.gene76478:CDS 40.0%
!! CAAATTTGGCCTTACCAGAA+AGG + chr8.1:31371685-31371704 None:intergenic 40.0%
!! GTTCTAGAAGAAAAGCTTCG+TGG - chr8.1:31371579-31371598 MS.gene76478:CDS 40.0%
!! TTCTTGATGACGTAACCTTC+TGG + chr8.1:31371611-31371630 None:intergenic 40.0%
! GATTGTAGCTCTGCTAAACG+CGG - chr8.1:31371728-31371747 MS.gene76478:CDS 45.0%
GCTTCGTGGAAAGATCCAGA+AGG - chr8.1:31371593-31371612 MS.gene76478:CDS 50.0%
! TGTAGCTCTGCTAAACGCGG+TGG - chr8.1:31371731-31371750 MS.gene76478:CDS 55.0%
GCTAAACGCGGTGGCGATCT+TGG - chr8.1:31371740-31371759 MS.gene76478:CDS 60.0%
TTCATCTCGCGGCGGCGCTG+AGG - chr8.1:31371523-31371542 MS.gene76478:CDS 70.0%
! TCGCGGCGGCGCTGAGGTTA+GGG - chr8.1:31371529-31371548 MS.gene76478:CDS 70.0%
! CTCGCGGCGGCGCTGAGGTT+AGG - chr8.1:31371528-31371547 MS.gene76478:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 31371518 31371799 31371518 ID=MS.gene76478
chr8.1 mRNA 31371518 31371799 31371518 ID=MS.gene76478.t1;Parent=MS.gene76478
chr8.1 exon 31371518 31371799 31371518 ID=MS.gene76478.t1.exon1;Parent=MS.gene76478.t1
chr8.1 CDS 31371518 31371799 31371518 ID=cds.MS.gene76478.t1;Parent=MS.gene76478.t1
Gene Sequence

>MS.gene76478

ATGTCTTCATCTCGCGGCGGCGCTGAGGTTAGGGCTTCACACATACTGATTAAACACGAAGGTTCTAGAAGAAAAGCTTCGTGGAAAGATCCAGAAGGTTACGTCATCAAGAACACTACTCGTGAAAGTGCCGTTGCTATTCTTACTTCAATGAAAGAGGATATCCTTTCTGGTAAGGCCAAATTTGAGGATCTTGCTTCGAGGTATTCTGATTGTAGCTCTGCTAAACGCGGTGGCGATCTTGGTAAGCAAATTTCAGATTCTTCAAATTCGTTTGTGTGA

Protein sequence

>MS.gene76478.t1

MSSSRGGAEVRASHILIKHEGSRRKASWKDPEGYVIKNTTRESAVAILTSMKEDILSGKAKFEDLASRYSDCSSAKRGGDLGKQISDSSNSFV