AlfalfaGEDB | MS.gene44260

M. sativa cultivar XinJiangDaYe / MS.gene44260

KEGG: K09578
GO: GO:0003674 GO:0003755 GO:0003824 GO:0016853 GO:0016859 GO:0140096
PFAM: PF00639.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44260.t1	XP_003629341.1	98.8	81	1	0	1	81	1	81	2.60E-35	157.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44260.t1	Q94G00	77.2	79	18	0	3	81	2	80	8.9e-29	127.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44260.t1	G7LCY6	98.8	81	1	0	1	81	1	81	1.8e-35	157.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049166	MS.gene44260	0.813782	2.15E-51	-1.69E-46
MS.gene049255	MS.gene44260	0.809558	1.78E-50	-1.69E-46
MS.gene049382	MS.gene44260	0.810386	1.18E-50	-1.69E-46
MS.gene049685	MS.gene44260	0.801098	1.06E-48	-1.69E-46
MS.gene049703	MS.gene44260	-0.800873	1.17E-48	-1.69E-46
MS.gene049756	MS.gene44260	0.837921	3.91E-57	-1.69E-46
MS.gene04984	MS.gene44260	0.8129	3.36E-51	-1.69E-46
MS.gene049887	MS.gene44260	0.821776	3.37E-53	-1.69E-46
MS.gene050084	MS.gene44260	0.804387	2.21E-49	-1.69E-46
MS.gene050085	MS.gene44260	0.832904	7.24E-56	-1.69E-46
MS.gene050114	MS.gene44260	0.842253	2.90E-58	-1.69E-46
MS.gene050277	MS.gene44260	0.847506	1.11E-59	-1.69E-46
MS.gene050317	MS.gene44260	0.829929	3.91E-55	-1.69E-46
MS.gene050320	MS.gene44260	0.800236	1.58E-48	-1.69E-46
MS.gene050386	MS.gene44260	0.801715	7.90E-49	-1.69E-46
MS.gene050437	MS.gene44260	0.804257	2.36E-49	-1.69E-46
MS.gene050689	MS.gene44260	0.836986	6.78E-57	-1.69E-46
MS.gene050735	MS.gene44260	0.800443	1.44E-48	-1.69E-46
MS.gene050752	MS.gene44260	0.808045	3.76E-50	-1.69E-46
MS.gene050762	MS.gene44260	0.812999	3.20E-51	-1.69E-46
MS.gene050763	MS.gene44260	0.811206	7.86E-51	-1.69E-46
MS.gene050815	MS.gene44260	0.803338	3.66E-49	-1.69E-46
MS.gene050838	MS.gene44260	0.866912	2.01E-65	-1.69E-46
MS.gene051003	MS.gene44260	0.819885	9.18E-53	-1.69E-46
MS.gene051063	MS.gene44260	0.816032	6.82E-52	-1.69E-46
MS.gene051102	MS.gene44260	0.801736	7.82E-49	-1.69E-46
MS.gene051233	MS.gene44260	0.809406	1.92E-50	-1.69E-46
MS.gene051243	MS.gene44260	0.832556	8.84E-56	-1.69E-46
MS.gene051245	MS.gene44260	0.86178	8.07E-64	-1.69E-46
MS.gene051328	MS.gene44260	0.838459	2.84E-57	-1.69E-46
MS.gene051343	MS.gene44260	0.826266	2.98E-54	-1.69E-46
MS.gene05134	MS.gene44260	0.80194	7.10E-49	-1.69E-46
MS.gene051451	MS.gene44260	0.815597	8.52E-52	-1.69E-46
MS.gene051746	MS.gene44260	0.822665	2.10E-53	-1.69E-46
MS.gene051748	MS.gene44260	0.842797	2.08E-58	-1.69E-46
MS.gene051756	MS.gene44260	0.833526	5.07E-56	-1.69E-46
MS.gene051757	MS.gene44260	0.832899	7.27E-56	-1.69E-46
MS.gene051850	MS.gene44260	0.821198	4.59E-53	-1.69E-46
MS.gene051903	MS.gene44260	0.824667	7.13E-54	-1.69E-46
MS.gene051975	MS.gene44260	0.820385	7.05E-53	-1.69E-46
MS.gene052133	MS.gene44260	0.803038	4.22E-49	-1.69E-46
MS.gene052286	MS.gene44260	0.818744	1.67E-52	-1.69E-46
MS.gene052397	MS.gene44260	0.826271	2.97E-54	-1.69E-46
MS.gene052434	MS.gene44260	0.822198	2.69E-53	-1.69E-46
MS.gene052665	MS.gene44260	0.814765	1.30E-51	-1.69E-46
MS.gene052856	MS.gene44260	0.831405	1.70E-55	-1.69E-46
MS.gene052858	MS.gene44260	0.80883	2.55E-50	-1.69E-46
MS.gene052860	MS.gene44260	0.806816	6.84E-50	-1.69E-46
MS.gene052947	MS.gene44260	0.815529	8.82E-52	-1.69E-46
MS.gene053058	MS.gene44260	0.860667	1.76E-63	-1.69E-46
MS.gene053109	MS.gene44260	0.844654	6.61E-59	-1.69E-46
MS.gene053381	MS.gene44260	0.816125	6.50E-52	-1.69E-46
MS.gene05380	MS.gene44260	0.805343	1.40E-49	-1.69E-46
MS.gene053952	MS.gene44260	0.809989	1.44E-50	-1.69E-46
MS.gene054403	MS.gene44260	0.800762	1.24E-48	-1.69E-46
MS.gene05462	MS.gene44260	0.806672	7.34E-50	-1.69E-46
MS.gene054823	MS.gene44260	0.82395	1.05E-53	-1.69E-46
MS.gene05517	MS.gene44260	0.801656	8.12E-49	-1.69E-46
MS.gene055311	MS.gene44260	0.848255	6.89E-60	-1.69E-46
MS.gene055314	MS.gene44260	0.805779	1.13E-49	-1.69E-46
MS.gene055424	MS.gene44260	0.837959	3.82E-57	-1.69E-46
MS.gene055441	MS.gene44260	0.8528	3.64E-61	-1.69E-46
MS.gene056097	MS.gene44260	0.834226	3.39E-56	-1.69E-46
MS.gene056181	MS.gene44260	0.844781	6.11E-59	-1.69E-46
MS.gene056432	MS.gene44260	0.835529	1.59E-56	-1.69E-46
MS.gene056580	MS.gene44260	0.858171	9.89E-63	-1.69E-46
MS.gene056631	MS.gene44260	0.839064	1.98E-57	-1.69E-46
MS.gene056760	MS.gene44260	0.829539	4.86E-55	-1.69E-46
MS.gene056787	MS.gene44260	0.853325	2.57E-61	-1.69E-46
MS.gene056986	MS.gene44260	0.802647	5.08E-49	-1.69E-46
MS.gene057057	MS.gene44260	0.854017	1.63E-61	-1.69E-46
MS.gene057094	MS.gene44260	0.852808	3.62E-61	-1.69E-46
MS.gene057140	MS.gene44260	0.846958	1.57E-59	-1.69E-46
MS.gene057234	MS.gene44260	0.856286	3.56E-62	-1.69E-46
MS.gene057360	MS.gene44260	0.813892	2.03E-51	-1.69E-46
MS.gene057451	MS.gene44260	-0.804131	2.50E-49	-1.69E-46
MS.gene057452	MS.gene44260	-0.824586	7.45E-54	-1.69E-46
MS.gene05781	MS.gene44260	0.80201	6.87E-49	-1.69E-46
MS.gene058054	MS.gene44260	0.832565	8.79E-56	-1.69E-46
MS.gene058055	MS.gene44260	0.852043	5.98E-61	-1.69E-46
MS.gene058084	MS.gene44260	0.807968	3.90E-50	-1.69E-46
MS.gene05809	MS.gene44260	0.806751	7.06E-50	-1.69E-46
MS.gene058126	MS.gene44260	0.810525	1.10E-50	-1.69E-46
MS.gene058182	MS.gene44260	0.810786	9.69E-51	-1.69E-46
MS.gene058238	MS.gene44260	0.860241	2.37E-63	-1.69E-46
MS.gene058638	MS.gene44260	0.818288	2.12E-52	-1.69E-46
MS.gene058639	MS.gene44260	0.826701	2.35E-54	-1.69E-46
MS.gene058646	MS.gene44260	0.833118	6.41E-56	-1.69E-46
MS.gene058654	MS.gene44260	0.828993	6.60E-55	-1.69E-46
MS.gene058758	MS.gene44260	0.835527	1.59E-56	-1.69E-46
MS.gene058906	MS.gene44260	0.869367	3.27E-66	-1.69E-46
MS.gene058976	MS.gene44260	0.803261	3.79E-49	-1.69E-46
MS.gene059171	MS.gene44260	0.818213	2.20E-52	-1.69E-46
MS.gene059360	MS.gene44260	0.821168	4.66E-53	-1.69E-46
MS.gene059725	MS.gene44260	0.802463	5.54E-49	-1.69E-46
MS.gene05978	MS.gene44260	0.823182	1.59E-53	-1.69E-46
MS.gene059898	MS.gene44260	0.821128	4.76E-53	-1.69E-46
MS.gene06001	MS.gene44260	0.849265	3.61E-60	-1.69E-46
MS.gene060084	MS.gene44260	0.821407	4.10E-53	-1.69E-46
MS.gene06010	MS.gene44260	0.827095	1.89E-54	-1.69E-46
MS.gene06011	MS.gene44260	0.807929	3.98E-50	-1.69E-46
MS.gene060296	MS.gene44260	0.829275	5.64E-55	-1.69E-46
MS.gene060297	MS.gene44260	0.817076	3.98E-52	-1.69E-46
MS.gene060463	MS.gene44260	0.85018	2.01E-60	-1.69E-46
MS.gene060465	MS.gene44260	0.859836	3.14E-63	-1.69E-46
MS.gene060563	MS.gene44260	0.816325	5.86E-52	-1.69E-46
MS.gene060637	MS.gene44260	0.811051	8.49E-51	-1.69E-46
MS.gene060702	MS.gene44260	0.836557	8.73E-57	-1.69E-46
MS.gene060869	MS.gene44260	0.819337	1.22E-52	-1.69E-46
MS.gene060870	MS.gene44260	0.816003	6.92E-52	-1.69E-46
MS.gene061006	MS.gene44260	0.827447	1.56E-54	-1.69E-46
MS.gene061055	MS.gene44260	0.800118	1.67E-48	-1.69E-46
MS.gene061145	MS.gene44260	0.813897	2.03E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44260.t1	MTR_8g076070	98.765	81	1	0	1	81	1	81	6.64e-54	163

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene44260.t1	AT2G18040	73.973	73	19	0	9	81	6	78	2.76e-35	116

Find 20 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGAAAGAGGATATCCTTTC+TGG	0.182475	8.1:-31385724	MS.gene44260:CDS
CGAAGCAAGATCCTCAAATT+TGG	0.289474	8.1:+31385696	None:intergenic
CTCGCGGCGGCGCTGAGGTT+AGG	0.301148	8.1:-31385864	MS.gene44260:CDS
TTCTTGATGACGTAACCTTC+TGG	0.327996	8.1:+31385784	None:intergenic
GCTAAACGCGGTGGCGATCT+TGG	0.368998	8.1:-31385652	None:intergenic
TCGCGGCGGCGCTGAGGTTA+GGG	0.411373	8.1:-31385863	MS.gene44260:CDS
TTCTGGTAAGGCCAAATTTG+AGG	0.428366	8.1:-31385707	MS.gene44260:CDS
CAAATTTGGCCTTACCAGAA+AGG	0.448271	8.1:+31385710	None:intergenic
TATTCTTACTTCAATGAAAG+AGG	0.454286	8.1:-31385737	MS.gene44260:CDS
AGAGGATATCCTTTCTGGTA+AGG	0.533949	8.1:-31385719	MS.gene44260:CDS
AATTTGAGGATCTTGCTTCG+AGG	0.550226	8.1:-31385693	MS.gene44260:CDS
GTTCTAGAAGAAAAGCTTCG+TGG	0.556909	8.1:-31385813	MS.gene44260:CDS
TTCATCTCGCGGCGGCGCTG+AGG	0.579649	8.1:-31385869	MS.gene44260:CDS
CATTGAAGTAAGAATAGCAA+CGG	0.597353	8.1:+31385744	None:intergenic
GCGAAAATGTCTTCATCTCG+CGG	0.645162	8.1:-31385880	None:intergenic
CACATACTGATTAAACACGA+AGG	0.672969	8.1:-31385835	MS.gene44260:CDS
TGTAGCTCTGCTAAACGCGG+TGG	0.678782	8.1:-31385661	MS.gene44260:CDS
GATTGTAGCTCTGCTAAACG+CGG	0.692633	8.1:-31385664	MS.gene44260:CDS
GCTTCGTGGAAAGATCCAGA+AGG	0.706542	8.1:-31385799	MS.gene44260:CDS
AAAATGTCTTCATCTCGCGG+CGG	0.772656	8.1:-31385877	MS.gene44260:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATTCTTACTTCAATGAAAG+AGG	-	chr8.1:31385790-31385809	MS.gene44260:CDS	25.0%
	CATTGAAGTAAGAATAGCAA+CGG	+	chr8.1:31385786-31385805	None:intergenic	30.0%
	ATGAAAGAGGATATCCTTTC+TGG	-	chr8.1:31385803-31385822	MS.gene44260:CDS	35.0%
!	CACATACTGATTAAACACGA+AGG	-	chr8.1:31385692-31385711	MS.gene44260:CDS	35.0%
	AATTTGAGGATCTTGCTTCG+AGG	-	chr8.1:31385834-31385853	MS.gene44260:CDS	40.0%
	CGAAGCAAGATCCTCAAATT+TGG	+	chr8.1:31385834-31385853	None:intergenic	40.0%
	TTCTGGTAAGGCCAAATTTG+AGG	-	chr8.1:31385820-31385839	MS.gene44260:CDS	40.0%
!	AGAGGATATCCTTTCTGGTA+AGG	-	chr8.1:31385808-31385827	MS.gene44260:CDS	40.0%
!!	CAAATTTGGCCTTACCAGAA+AGG	+	chr8.1:31385820-31385839	None:intergenic	40.0%
!!	GTTCTAGAAGAAAAGCTTCG+TGG	-	chr8.1:31385714-31385733	MS.gene44260:CDS	40.0%
!!	TTCTTGATGACGTAACCTTC+TGG	+	chr8.1:31385746-31385765	None:intergenic	40.0%
!	GATTGTAGCTCTGCTAAACG+CGG	-	chr8.1:31385863-31385882	MS.gene44260:CDS	45.0%
	GCTTCGTGGAAAGATCCAGA+AGG	-	chr8.1:31385728-31385747	MS.gene44260:CDS	50.0%
!	TGTAGCTCTGCTAAACGCGG+TGG	-	chr8.1:31385866-31385885	MS.gene44260:CDS	55.0%
	TTCATCTCGCGGCGGCGCTG+AGG	-	chr8.1:31385658-31385677	MS.gene44260:CDS	70.0%
!	TCGCGGCGGCGCTGAGGTTA+GGG	-	chr8.1:31385664-31385683	MS.gene44260:CDS	70.0%
!	CTCGCGGCGGCGCTGAGGTT+AGG	-	chr8.1:31385663-31385682	MS.gene44260:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	31385653	31385896	31385653	ID=MS.gene44260
chr8.1	mRNA	31385653	31385896	31385653	ID=MS.gene44260.t1;Parent=MS.gene44260
chr8.1	exon	31385653	31385896	31385653	ID=MS.gene44260.t1.exon1;Parent=MS.gene44260.t1
chr8.1	CDS	31385653	31385896	31385653	ID=cds.MS.gene44260.t1;Parent=MS.gene44260.t1

Gene Sequence	>MS.gene44260 ATGTCTTCATCTCGCGGCGGCGCTGAGGTTAGGGCTTCACACATACTGATTAAACACGAAGGTTCTAGAAGAAAAGCTTCGTGGAAAGATCCAGAAGGTTACGTCATCAAGAACACTACTCGTGAAAGTGCCGTTGCTATTCTTACTTCAATGAAAGAGGATATCCTTTCTGGTAAGGCCAAATTTGAGGATCTTGCTTCGAGGTATTCTGATTGTAGCTCTGCTAAACGCGGTGGCGATCTTG
Protein sequence	>MS.gene44260.t1 MSSSRGGAEVRASHILIKHEGSRRKASWKDPEGYVIKNTTRESAVAILTSMKEDILSGKAKFEDLASRYSDCSSAKRGGDL