Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061271.t1 | XP_003609775.2 | 98.2 | 165 | 3 | 0 | 1 | 165 | 1 | 165 | 1.10E-88 | 335.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061271.t1 | Q1G3T1 | 47.4 | 97 | 48 | 2 | 67 | 161 | 80 | 175 | 3.7e-21 | 102.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061271.t1 | G7JNW5 | 98.2 | 165 | 3 | 0 | 1 | 165 | 1 | 165 | 7.7e-89 | 335.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene061271 | 0.809399 | 1.93E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene061271 | 0.844227 | 8.61E-59 | -1.69E-46 |
| MS.gene049285 | MS.gene061271 | 0.809615 | 1.73E-50 | -1.69E-46 |
| MS.gene050163 | MS.gene061271 | 0.800245 | 1.58E-48 | -1.69E-46 |
| MS.gene050612 | MS.gene061271 | 0.802322 | 5.93E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene061271 | 0.808111 | 3.64E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene061271 | 0.811672 | 6.23E-51 | -1.69E-46 |
| MS.gene051810 | MS.gene061271 | 0.809439 | 1.89E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene061271 | 0.838431 | 2.89E-57 | -1.69E-46 |
| MS.gene052271 | MS.gene061271 | 0.807018 | 6.20E-50 | -1.69E-46 |
| MS.gene052379 | MS.gene061271 | 0.800628 | 1.32E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene061271 | 0.80566 | 1.20E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene061271 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene053480 | MS.gene061271 | 0.802001 | 6.90E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene061271 | 0.803529 | 3.34E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene061271 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene055206 | MS.gene061271 | 0.857784 | 1.29E-62 | -1.69E-46 |
| MS.gene055362 | MS.gene061271 | 0.807345 | 5.29E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene061271 | 0.806208 | 9.20E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene061271 | 0.809347 | 1.98E-50 | -1.69E-46 |
| MS.gene056657 | MS.gene061271 | 0.805579 | 1.25E-49 | -1.69E-46 |
| MS.gene057196 | MS.gene061271 | 0.835245 | 1.88E-56 | -1.69E-46 |
| MS.gene057332 | MS.gene061271 | 0.800552 | 1.37E-48 | -1.69E-46 |
| MS.gene057658 | MS.gene061271 | 0.805755 | 1.15E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene061271 | 0.827654 | 1.39E-54 | -1.69E-46 |
| MS.gene059022 | MS.gene061271 | 0.80073 | 1.26E-48 | -1.69E-46 |
| MS.gene059023 | MS.gene061271 | 0.800015 | 1.76E-48 | -1.69E-46 |
| MS.gene05934 | MS.gene061271 | 0.821583 | 3.74E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene061271 | 0.815633 | 8.37E-52 | -1.69E-46 |
| MS.gene059756 | MS.gene061271 | 0.806086 | 9.76E-50 | -1.69E-46 |
| MS.gene059902 | MS.gene061271 | 0.837438 | 5.20E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061271.t1 | MTR_4g122090 | 98.182 | 165 | 3 | 0 | 1 | 165 | 1 | 165 | 7.57e-120 | 335 |
| MS.gene061271.t1 | MTR_2g034150 | 72.152 | 158 | 42 | 2 | 9 | 165 | 5 | 161 | 1.16e-80 | 236 |
| MS.gene061271.t1 | MTR_2g025290 | 61.856 | 97 | 35 | 2 | 68 | 164 | 132 | 226 | 5.53e-35 | 122 |
| MS.gene061271.t1 | MTR_3g098960 | 50.000 | 98 | 46 | 2 | 67 | 162 | 91 | 187 | 1.23e-28 | 104 |
| MS.gene061271.t1 | MTR_3g098960 | 50.000 | 98 | 46 | 2 | 67 | 162 | 127 | 223 | 3.92e-28 | 104 |
| MS.gene061271.t1 | MTR_5g096130 | 48.454 | 97 | 47 | 2 | 67 | 161 | 56 | 151 | 1.29e-26 | 98.6 |
| MS.gene061271.t1 | MTR_8g096400 | 50.602 | 83 | 40 | 1 | 81 | 163 | 1 | 82 | 3.27e-26 | 95.5 |
| MS.gene061271.t1 | MTR_2g025320 | 61.538 | 52 | 19 | 1 | 68 | 119 | 21 | 71 | 1.52e-16 | 70.5 |
| MS.gene061271.t1 | MTR_2g025350 | 39.024 | 82 | 44 | 2 | 37 | 118 | 10 | 85 | 1.03e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061271.t1 | AT1G32583 | 46.939 | 98 | 49 | 2 | 66 | 161 | 79 | 175 | 1.79e-27 | 101 |
| MS.gene061271.t1 | AT1G32583 | 46.939 | 98 | 49 | 2 | 66 | 161 | 79 | 175 | 1.79e-27 | 101 |
| MS.gene061271.t1 | AT4G24972 | 46.316 | 95 | 48 | 2 | 67 | 159 | 77 | 170 | 1.12e-24 | 94.4 |
Find 30 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATTTGACTAGAGGTTAA+AGG | 0.240600 | 8.3:+6495220 | None:intergenic |
| GATAGTCTCTTGAACAAGTT+AGG | 0.283613 | 8.3:+6495274 | None:intergenic |
| ACAATTTGCACTACATACTT+AGG | 0.355175 | 8.3:+6495379 | None:intergenic |
| CAGTGAAGGTGGATATTATA+TGG | 0.374593 | 8.3:+6495328 | None:intergenic |
| AGCAATGTTGAAATACATTC+AGG | 0.391870 | 8.3:-6495751 | MS.gene061271:intron |
| CAAAGTAGAGAGACTCTAAC+TGG | 0.402226 | 8.3:-6495406 | MS.gene061271:CDS |
| GTGTGCATGTTCTGCTTTCA+CGG | 0.419030 | 8.3:+6495581 | None:intergenic |
| TTTGATGATTGTTTGGTGAA+TGG | 0.424865 | 8.3:-6495250 | MS.gene061271:CDS |
| AAGAGGATAAACAAAAGAAT+TGG | 0.440347 | 8.3:+6495183 | None:intergenic |
| CAATTTGCACTACATACTTA+GGG | 0.446648 | 8.3:+6495380 | None:intergenic |
| ACCATGCCATGAAAATTTAG+TGG | 0.450215 | 8.3:+6495551 | None:intergenic |
| ATATCCACCTTCACTGTGGC+TGG | 0.466175 | 8.3:-6495321 | MS.gene061271:CDS |
| GAATGCAAAATCTGTGTTGA+AGG | 0.471618 | 8.3:+6495639 | None:intergenic |
| ATAGTCTCTTGAACAAGTTA+GGG | 0.506632 | 8.3:+6495275 | None:intergenic |
| TGAAGCAGAGGAAGAACAGA+GGG | 0.544265 | 8.3:+6495791 | None:intergenic |
| TCTTTCAATGAAGACAGAAA+CGG | 0.559717 | 8.3:-6495618 | MS.gene061271:CDS |
| GAATCTAATGATTTGACTAG+AGG | 0.560845 | 8.3:+6495213 | None:intergenic |
| CAAACCAGCCACAGTGAAGG+TGG | 0.574734 | 8.3:+6495317 | None:intergenic |
| TTGGCAGACTTGAGTGCAAG+AGG | 0.575548 | 8.3:+6495166 | None:intergenic |
| GATGATTGTTTGGTGAATGG+AGG | 0.585298 | 8.3:-6495247 | MS.gene061271:CDS |
| AATGCAAAATCTGTGTTGAA+GGG | 0.592176 | 8.3:+6495640 | None:intergenic |
| TCAACATTGCTAAGCACAAG+GGG | 0.606698 | 8.3:+6495763 | None:intergenic |
| ATGAAGCAGAGGAAGAACAG+AGG | 0.607052 | 8.3:+6495790 | None:intergenic |
| TTCAACATTGCTAAGCACAA+GGG | 0.607430 | 8.3:+6495762 | None:intergenic |
| AACATTGCTAAGCACAAGGG+GGG | 0.613965 | 8.3:+6495765 | None:intergenic |
| TTTCAACATTGCTAAGCACA+AGG | 0.617257 | 8.3:+6495761 | None:intergenic |
| AAGCAAACCAGCCACAGTGA+AGG | 0.638291 | 8.3:+6495314 | None:intergenic |
| CAACATTGCTAAGCACAAGG+GGG | 0.674413 | 8.3:+6495764 | None:intergenic |
| TATAATATCCACCTTCACTG+TGG | 0.711676 | 8.3:-6495325 | MS.gene061271:CDS |
| GAAGCAGAGGAAGAACAGAG+GGG | 0.773738 | 8.3:+6495792 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATTTTTGGTTTTGATT+AGG | - | chr8.3:6495507-6495526 | MS.gene061271:intron | 15.0% |
| !! | CAAAAATAAATAATCATCTC+AGG | + | chr8.3:6495498-6495517 | None:intergenic | 20.0% |
| !!! | CTGAGATGATTATTTATTTT+TGG | - | chr8.3:6495496-6495515 | MS.gene061271:intron | 20.0% |
| ! | AAGAGGATAAACAAAAGAAT+TGG | + | chr8.3:6495787-6495806 | None:intergenic | 25.0% |
| !! | TCTCAGGTTTAGAATATAAA+AGG | + | chr8.3:6495482-6495501 | None:intergenic | 25.0% |
| !!! | ACTATCTTTTGATGATTGTT+TGG | - | chr8.3:6495710-6495729 | MS.gene061271:intron | 25.0% |
| AATGCAAAATCTGTGTTGAA+GGG | + | chr8.3:6495330-6495349 | None:intergenic | 30.0% | |
| ACAATTTGCACTACATACTT+AGG | + | chr8.3:6495591-6495610 | None:intergenic | 30.0% | |
| AGCAATGTTGAAATACATTC+AGG | - | chr8.3:6495216-6495235 | MS.gene061271:CDS | 30.0% | |
| ATAGTCTCTTGAACAAGTTA+GGG | + | chr8.3:6495695-6495714 | None:intergenic | 30.0% | |
| ATGATTTGACTAGAGGTTAA+AGG | + | chr8.3:6495750-6495769 | None:intergenic | 30.0% | |
| ATGTTTATCATTGTGTTAGC+AGG | - | chr8.3:6495304-6495323 | MS.gene061271:CDS | 30.0% | |
| CAATTTGCACTACATACTTA+GGG | + | chr8.3:6495590-6495609 | None:intergenic | 30.0% | |
| GAATCTAATGATTTGACTAG+AGG | + | chr8.3:6495757-6495776 | None:intergenic | 30.0% | |
| TCTTTCAATGAAGACAGAAA+CGG | - | chr8.3:6495349-6495368 | MS.gene061271:CDS | 30.0% | |
| ! | CTACTTCCACTAAATTTTCA+TGG | - | chr8.3:6495410-6495429 | MS.gene061271:CDS | 30.0% |
| ! | TTTGATGATTGTTTGGTGAA+TGG | - | chr8.3:6495717-6495736 | MS.gene061271:intron | 30.0% |
| ACCATGCCATGAAAATTTAG+TGG | + | chr8.3:6495419-6495438 | None:intergenic | 35.0% | |
| CAGTGAAGGTGGATATTATA+TGG | + | chr8.3:6495642-6495661 | None:intergenic | 35.0% | |
| GAATGCAAAATCTGTGTTGA+AGG | + | chr8.3:6495331-6495350 | None:intergenic | 35.0% | |
| GATAGTCTCTTGAACAAGTT+AGG | + | chr8.3:6495696-6495715 | None:intergenic | 35.0% | |
| TATAATATCCACCTTCACTG+TGG | - | chr8.3:6495642-6495661 | MS.gene061271:CDS | 35.0% | |
| TTCAACATTGCTAAGCACAA+GGG | + | chr8.3:6495208-6495227 | None:intergenic | 35.0% | |
| TTTCAACATTGCTAAGCACA+AGG | + | chr8.3:6495209-6495228 | None:intergenic | 35.0% | |
| ! | TCCACTAAATTTTCATGGCA+TGG | - | chr8.3:6495415-6495434 | MS.gene061271:CDS | 35.0% |
| CAAAGTAGAGAGACTCTAAC+TGG | - | chr8.3:6495561-6495580 | MS.gene061271:CDS | 40.0% | |
| TCAACATTGCTAAGCACAAG+GGG | + | chr8.3:6495207-6495226 | None:intergenic | 40.0% | |
| ! | GATGATTGTTTGGTGAATGG+AGG | - | chr8.3:6495720-6495739 | MS.gene061271:intron | 40.0% |
| AACATTGCTAAGCACAAGGG+GGG | + | chr8.3:6495205-6495224 | None:intergenic | 45.0% | |
| ATGAAGCAGAGGAAGAACAG+AGG | + | chr8.3:6495180-6495199 | None:intergenic | 45.0% | |
| CAACATTGCTAAGCACAAGG+GGG | + | chr8.3:6495206-6495225 | None:intergenic | 45.0% | |
| GTGTGCATGTTCTGCTTTCA+CGG | + | chr8.3:6495389-6495408 | None:intergenic | 45.0% | |
| TGAAGCAGAGGAAGAACAGA+GGG | + | chr8.3:6495179-6495198 | None:intergenic | 45.0% | |
| AAGCAAACCAGCCACAGTGA+AGG | + | chr8.3:6495656-6495675 | None:intergenic | 50.0% | |
| ATATCCACCTTCACTGTGGC+TGG | - | chr8.3:6495646-6495665 | MS.gene061271:intron | 50.0% | |
| GAAGCAGAGGAAGAACAGAG+GGG | + | chr8.3:6495178-6495197 | None:intergenic | 50.0% | |
| TTGGCAGACTTGAGTGCAAG+AGG | + | chr8.3:6495804-6495823 | None:intergenic | 50.0% | |
| CAAACCAGCCACAGTGAAGG+TGG | + | chr8.3:6495653-6495672 | None:intergenic | 55.0% | |
| CAAGGGGGGTTATGAAGCAG+AGG | + | chr8.3:6495191-6495210 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 6495156 | 6495833 | 6495156 | ID=MS.gene061271 |
| chr8.3 | mRNA | 6495156 | 6495833 | 6495156 | ID=MS.gene061271.t1;Parent=MS.gene061271 |
| chr8.3 | exon | 6495752 | 6495833 | 6495752 | ID=MS.gene061271.t1.exon1;Parent=MS.gene061271.t1 |
| chr8.3 | CDS | 6495752 | 6495833 | 6495752 | ID=cds.MS.gene061271.t1;Parent=MS.gene061271.t1 |
| chr8.3 | exon | 6495553 | 6495663 | 6495553 | ID=MS.gene061271.t1.exon2;Parent=MS.gene061271.t1 |
| chr8.3 | CDS | 6495553 | 6495663 | 6495553 | ID=cds.MS.gene061271.t1;Parent=MS.gene061271.t1 |
| chr8.3 | exon | 6495156 | 6495460 | 6495156 | ID=MS.gene061271.t1.exon3;Parent=MS.gene061271.t1 |
| chr8.3 | CDS | 6495156 | 6495460 | 6495156 | ID=cds.MS.gene061271.t1;Parent=MS.gene061271.t1 |
| Gene Sequence |
| Protein sequence |
