Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062183.t1 | KEH42885.1 | 97 | 99 | 1 | 2 | 1 | 98 | 175 | 272 | 1.00E-46 | 195.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062183.t1 | A0A072VMR5 | 97.0 | 99 | 1 | 2 | 1 | 98 | 175 | 272 | 7.4e-47 | 195.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene062183 | 0.820151 | 7.98E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene062183 | 0.801276 | 9.72E-49 | -1.69E-46 |
| MS.gene049350 | MS.gene062183 | 0.838636 | 2.56E-57 | -1.69E-46 |
| MS.gene049711 | MS.gene062183 | 0.829551 | 4.83E-55 | -1.69E-46 |
| MS.gene049807 | MS.gene062183 | 0.824591 | 7.43E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene062183 | 0.800857 | 1.18E-48 | -1.69E-46 |
| MS.gene050561 | MS.gene062183 | 0.805409 | 1.35E-49 | -1.69E-46 |
| MS.gene050562 | MS.gene062183 | 0.802035 | 6.79E-49 | -1.69E-46 |
| MS.gene051201 | MS.gene062183 | 0.820096 | 8.21E-53 | -1.69E-46 |
| MS.gene051279 | MS.gene062183 | 0.82704 | 1.95E-54 | -1.69E-46 |
| MS.gene051719 | MS.gene062183 | 0.8014 | 9.17E-49 | -1.69E-46 |
| MS.gene051720 | MS.gene062183 | 0.810034 | 1.41E-50 | -1.69E-46 |
| MS.gene051744 | MS.gene062183 | 0.803976 | 2.70E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene062183 | 0.805135 | 1.54E-49 | -1.69E-46 |
| MS.gene052463 | MS.gene062183 | 0.800666 | 1.29E-48 | -1.69E-46 |
| MS.gene052514 | MS.gene062183 | 0.805836 | 1.10E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene062183 | 0.81919 | 1.32E-52 | -1.69E-46 |
| MS.gene053144 | MS.gene062183 | 0.835356 | 1.76E-56 | -1.69E-46 |
| MS.gene054178 | MS.gene062183 | 0.821552 | 3.80E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene062183 | 0.823325 | 1.47E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene062183 | 0.827884 | 1.22E-54 | -1.69E-46 |
| MS.gene055083 | MS.gene062183 | 0.822314 | 2.53E-53 | -1.69E-46 |
| MS.gene055103 | MS.gene062183 | 0.811112 | 8.24E-51 | -1.69E-46 |
| MS.gene055537 | MS.gene062183 | 0.808395 | 3.16E-50 | -1.69E-46 |
| MS.gene055891 | MS.gene062183 | 0.801236 | 9.90E-49 | -1.69E-46 |
| MS.gene056050 | MS.gene062183 | 0.819575 | 1.08E-52 | -1.69E-46 |
| MS.gene056507 | MS.gene062183 | 0.805237 | 1.47E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene062183 | 0.817264 | 3.61E-52 | -1.69E-46 |
| MS.gene057082 | MS.gene062183 | 0.803111 | 4.07E-49 | -1.69E-46 |
| MS.gene057132 | MS.gene062183 | 0.802846 | 4.62E-49 | -1.69E-46 |
| MS.gene058668 | MS.gene062183 | 0.828413 | 9.11E-55 | -1.69E-46 |
| MS.gene05894 | MS.gene062183 | 0.8092 | 2.13E-50 | -1.69E-46 |
| MS.gene059570 | MS.gene062183 | 0.816101 | 6.58E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene062183.t1 | MTR_1g077600 | 97.727 | 88 | 1 | 1 | 1 | 88 | 175 | 261 | 4.42e-57 | 177 |
| MS.gene062183.t1 | MTR_1g077600 | 97.727 | 88 | 1 | 1 | 1 | 88 | 175 | 261 | 6.06e-57 | 177 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAACCCAACAATATTGGATT+TGG | 0.276934 | 1.1:+58148753 | MS.gene062183:CDS |
| TACAAATAACCCAACAATAT+TGG | 0.279491 | 1.1:+58148747 | MS.gene062183:CDS |
| CCAATGAATTTCTGTTTAGA+TGG | 0.293399 | 1.1:+58148619 | MS.gene062183:CDS |
| TTCATGCCTGCTCAAGTTCA+AGG | 0.318142 | 1.1:+58148562 | None:intergenic |
| AAGGATATTCAACAGGGATT+TGG | 0.319140 | 1.1:+58148803 | MS.gene062183:CDS |
| TTTGGATCAGAATAATAATA+AGG | 0.356949 | 1.1:+58148771 | MS.gene062183:CDS |
| GATCAACAAGGATATTCAAC+AGG | 0.388223 | 1.1:+58148796 | MS.gene062183:CDS |
| GATCCAAATCCAATATTGTT+GGG | 0.402423 | 1.1:-58148756 | None:intergenic |
| TTCTGTTTAGATGGGATCTC+AGG | 0.420885 | 1.1:+58148628 | MS.gene062183:CDS |
| GGGATTTGGACTGGAATGTT+GGG | 0.441281 | 1.1:+58148817 | MS.gene062183:CDS |
| CCTGCTCAAGTTCAAGGTTG+TGG | 0.443611 | 1.1:+58148568 | MS.gene062183:CDS |
| CCATCTAAACAGAAATTCAT+TGG | 0.447886 | 1.1:-58148619 | None:intergenic |
| ACTGGAATGTTGGGAGGAGG+AGG | 0.456613 | 1.1:+58148826 | MS.gene062183:CDS |
| TGATCCAAATCCAATATTGT+TGG | 0.463007 | 1.1:-58148757 | None:intergenic |
| AGGGATTTGGACTGGAATGT+TGG | 0.470831 | 1.1:+58148816 | MS.gene062183:CDS |
| CAATGAATTTCTGTTTAGAT+GGG | 0.475119 | 1.1:+58148620 | MS.gene062183:CDS |
| TGGACTGGAATGTTGGGAGG+AGG | 0.475312 | 1.1:+58148823 | MS.gene062183:CDS |
| GGAATGTTGGGAGGAGGAGG+AGG | 0.484270 | 1.1:+58148829 | MS.gene062183:CDS |
| ACATGTTTCAAATCCTCAAA+AGG | 0.498889 | 1.1:-58148718 | None:intergenic |
| AAGAGACAATTAGTGAAGGA+AGG | 0.513089 | 1.1:+58148686 | MS.gene062183:CDS |
| TATTCAACAGGGATTTGGAC+TGG | 0.519358 | 1.1:+58148808 | MS.gene062183:CDS |
| AGAGACAATTAGTGAAGGAA+GGG | 0.538775 | 1.1:+58148687 | MS.gene062183:CDS |
| CCACAACCTTGAACTTGAGC+AGG | 0.540402 | 1.1:-58148568 | None:intergenic |
| TTAGATGGGATCTCAGGAAG+TGG | 0.574889 | 1.1:+58148634 | MS.gene062183:CDS |
| TATGCAAGTACTCTTGATCA+TGG | 0.590246 | 1.1:+58148658 | MS.gene062183:CDS |
| ATTTGGACTGGAATGTTGGG+AGG | 0.599027 | 1.1:+58148820 | MS.gene062183:CDS |
| ACTTGCTTAAAATCTTGCAA+AGG | 0.603902 | 1.1:-58148595 | None:intergenic |
| GTTCAAGAGACAATTAGTGA+AGG | 0.630784 | 1.1:+58148682 | MS.gene062183:CDS |
| AATAATAAGGAAGATCAACA+AGG | 0.712044 | 1.1:+58148784 | MS.gene062183:CDS |
| ATCAACAAGGATATTCAACA+GGG | 0.718207 | 1.1:+58148797 | MS.gene062183:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTGGATCAGAATAATAATA+AGG | + | chr1.1:58148771-58148790 | MS.gene062183:CDS | 20.0% |
| ! | AATAATAAGGAAGATCAACA+AGG | + | chr1.1:58148784-58148803 | MS.gene062183:CDS | 25.0% |
| ! | CAATGAATTTCTGTTTAGAT+GGG | + | chr1.1:58148620-58148639 | MS.gene062183:CDS | 25.0% |
| ! | TACAAATAACCCAACAATAT+TGG | + | chr1.1:58148747-58148766 | MS.gene062183:CDS | 25.0% |
| ACATGTTTCAAATCCTCAAA+AGG | - | chr1.1:58148721-58148740 | None:intergenic | 30.0% | |
| ACTTGCTTAAAATCTTGCAA+AGG | - | chr1.1:58148598-58148617 | None:intergenic | 30.0% | |
| ATCAACAAGGATATTCAACA+GGG | + | chr1.1:58148797-58148816 | MS.gene062183:CDS | 30.0% | |
| CCAATGAATTTCTGTTTAGA+TGG | + | chr1.1:58148619-58148638 | MS.gene062183:CDS | 30.0% | |
| CCATCTAAACAGAAATTCAT+TGG | - | chr1.1:58148622-58148641 | None:intergenic | 30.0% | |
| GATCCAAATCCAATATTGTT+GGG | - | chr1.1:58148759-58148778 | None:intergenic | 30.0% | |
| TAACCCAACAATATTGGATT+TGG | + | chr1.1:58148753-58148772 | MS.gene062183:CDS | 30.0% | |
| TGATCCAAATCCAATATTGT+TGG | - | chr1.1:58148760-58148779 | None:intergenic | 30.0% | |
| AAGAGACAATTAGTGAAGGA+AGG | + | chr1.1:58148686-58148705 | MS.gene062183:CDS | 35.0% | |
| AAGGATATTCAACAGGGATT+TGG | + | chr1.1:58148803-58148822 | MS.gene062183:CDS | 35.0% | |
| AGAGACAATTAGTGAAGGAA+GGG | + | chr1.1:58148687-58148706 | MS.gene062183:CDS | 35.0% | |
| GATCAACAAGGATATTCAAC+AGG | + | chr1.1:58148796-58148815 | MS.gene062183:CDS | 35.0% | |
| GTTCAAGAGACAATTAGTGA+AGG | + | chr1.1:58148682-58148701 | MS.gene062183:CDS | 35.0% | |
| TATGCAAGTACTCTTGATCA+TGG | + | chr1.1:58148658-58148677 | MS.gene062183:CDS | 35.0% | |
| !!! | AAGGGTTTTGTTTCCTTTTG+AGG | + | chr1.1:58148705-58148724 | MS.gene062183:CDS | 35.0% |
| TATTCAACAGGGATTTGGAC+TGG | + | chr1.1:58148808-58148827 | MS.gene062183:CDS | 40.0% | |
| TTCTGTTTAGATGGGATCTC+AGG | + | chr1.1:58148628-58148647 | MS.gene062183:CDS | 40.0% | |
| TTAGATGGGATCTCAGGAAG+TGG | + | chr1.1:58148634-58148653 | MS.gene062183:CDS | 45.0% | |
| ! | AGGGATTTGGACTGGAATGT+TGG | + | chr1.1:58148816-58148835 | MS.gene062183:CDS | 45.0% |
| ! | ATTTGGACTGGAATGTTGGG+AGG | + | chr1.1:58148820-58148839 | MS.gene062183:CDS | 45.0% |
| ! | GGGATTTGGACTGGAATGTT+GGG | + | chr1.1:58148817-58148836 | MS.gene062183:CDS | 45.0% |
| CCACAACCTTGAACTTGAGC+AGG | - | chr1.1:58148571-58148590 | None:intergenic | 50.0% | |
| CCTGCTCAAGTTCAAGGTTG+TGG | + | chr1.1:58148568-58148587 | MS.gene062183:CDS | 50.0% | |
| ACTGGAATGTTGGGAGGAGG+AGG | + | chr1.1:58148826-58148845 | MS.gene062183:CDS | 55.0% | |
| ! | TGGACTGGAATGTTGGGAGG+AGG | + | chr1.1:58148823-58148842 | MS.gene062183:CDS | 55.0% |
| GGAATGTTGGGAGGAGGAGG+AGG | + | chr1.1:58148829-58148848 | MS.gene062183:CDS | 60.0% | |
| TGGGAGGAGGAGGAGGATCA+TGG | + | chr1.1:58148836-58148855 | MS.gene062183:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 58148565 | 58148861 | 58148565 | ID=MS.gene062183 |
| chr1.1 | mRNA | 58148565 | 58148861 | 58148565 | ID=MS.gene062183.t1;Parent=MS.gene062183 |
| chr1.1 | exon | 58148565 | 58148861 | 58148565 | ID=MS.gene062183.t1.exon1;Parent=MS.gene062183.t1 |
| chr1.1 | CDS | 58148565 | 58148861 | 58148565 | ID=cds.MS.gene062183.t1;Parent=MS.gene062183.t1 |
| Gene Sequence |
| Protein sequence |
