AlfalfaGEDB | MS.gene06298

M. sativa cultivar XinJiangDaYe / MS.gene06298

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06298.t1	XP_003602727.1	89.8	108	2	2	1	108	1	99	2.40E-44	188

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06298.t1	B7FGG5	89.8	108	2	2	1	108	1	99	1.7e-44	188.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049181	MS.gene06298	0.882656	8.68E-71	-1.69E-46
MS.gene049182	MS.gene06298	0.817099	3.93E-52	-1.69E-46
MS.gene050416	MS.gene06298	0.802079	6.65E-49	-1.69E-46
MS.gene050883	MS.gene06298	0.800294	1.54E-48	-1.69E-46
MS.gene051367	MS.gene06298	0.814822	1.27E-51	-1.69E-46
MS.gene051959	MS.gene06298	0.82346	1.37E-53	-1.69E-46
MS.gene051960	MS.gene06298	0.830437	2.94E-55	-1.69E-46
MS.gene052091	MS.gene06298	0.823732	1.18E-53	-1.69E-46
MS.gene053083	MS.gene06298	0.81579	7.72E-52	-1.69E-46
MS.gene054616	MS.gene06298	0.800273	1.56E-48	-1.69E-46
MS.gene054638	MS.gene06298	-0.813629	2.32E-51	-1.69E-46
MS.gene055150	MS.gene06298	0.842876	1.98E-58	-1.69E-46
MS.gene055549	MS.gene06298	0.835148	1.99E-56	-1.69E-46
MS.gene055550	MS.gene06298	0.833146	6.30E-56	-1.69E-46
MS.gene055634	MS.gene06298	-0.828177	1.04E-54	-1.69E-46
MS.gene055849	MS.gene06298	-0.810062	1.39E-50	-1.69E-46
MS.gene056185	MS.gene06298	0.800511	1.39E-48	-1.69E-46
MS.gene056530	MS.gene06298	0.828431	9.03E-55	-1.69E-46
MS.gene057001	MS.gene06298	0.811025	8.61E-51	-1.69E-46
MS.gene057148	MS.gene06298	-0.838311	3.10E-57	-1.69E-46
MS.gene058246	MS.gene06298	0.803957	2.72E-49	-1.69E-46
MS.gene058494	MS.gene06298	0.801687	8.01E-49	-1.69E-46
MS.gene05884	MS.gene06298	0.808304	3.31E-50	-1.69E-46
MS.gene060975	MS.gene06298	0.80093	1.14E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06298.t1	MTR_3g098390	89.815	108	2	2	1	108	1	99	1.11e-65	193

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene06298.t1	AT2G44010	35.345	116	50	5	1	107	1	100	4.29e-12	57.8

Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTGTTGGAGATAATTT+TGG	0.176993	3.1:-77992968	MS.gene06298:CDS
CAGTATGAGGTTATTTCTTT+AGG	0.254934	3.1:-77993100	MS.gene06298:CDS
GCTTCAGATAATTACAATAT+TGG	0.370686	3.1:-77993004	MS.gene06298:CDS
TTTCATGATCAGACAACAAC+TGG	0.416618	3.1:-77992932	MS.gene06298:CDS
TAGAACAAGACGACAGTATG+AGG	0.483439	3.1:-77993113	MS.gene06298:CDS
TGAAGTGGTTGTTGAGTTTG+AGG	0.489679	3.1:+77993043	None:intergenic
CACAGGAGACATAAATCTGT+TGG	0.502696	3.1:-77992980	MS.gene06298:CDS
TATGAGGTTATTTCTTTAGG+AGG	0.514646	3.1:-77993097	MS.gene06298:CDS
GTGGTTGTTGAGTTTGAGGG+TGG	0.516517	3.1:+77993047	None:intergenic
GAAGTGGTTGTTGAGTTTGA+GGG	0.525781	3.1:+77993044	None:intergenic
ATATTGTAATTATCTGAAGC+TGG	0.533222	3.1:+77993007	None:intergenic
TTGATTGCTTTATACACAAG+AGG	0.566790	3.1:+77993142	None:intergenic
ATAATTACAATATTGGTCAC+AGG	0.578276	3.1:-77992997	MS.gene06298:CDS
GGTATTTGATTATGATGAAG+TGG	0.585229	3.1:+77993028	None:intergenic
ACAATAAGAAGTGTTATGGA+AGG	0.606553	3.1:-77993172	None:intergenic
AGGAAGCTACAACATAAGCA+TGG	0.662553	3.1:-77993077	MS.gene06298:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACAAGTTTTTTTGAAGAATT+AGG	+	chr3.1:77993114-77993133	None:intergenic	20.0%
!	ATAATTACAATATTGGTCAC+AGG	-	chr3.1:77993013-77993032	MS.gene06298:CDS	25.0%
!	ATTCTTCAAAAAAACTTGTC+AGG	-	chr3.1:77993115-77993134	MS.gene06298:CDS	25.0%
!	GCTTCAGATAATTACAATAT+TGG	-	chr3.1:77993006-77993025	MS.gene06298:CDS	25.0%
!!	AAATCTGTTGGAGATAATTT+TGG	-	chr3.1:77993042-77993061	MS.gene06298:CDS	25.0%
!!	AGAATGTTTTCATGTATCAA+GGG	-	chr3.1:77993150-77993169	MS.gene06298:CDS	25.0%
!!	ATATTGTAATTATCTGAAGC+TGG	+	chr3.1:77993006-77993025	None:intergenic	25.0%
!!	TAGAATGTTTTCATGTATCA+AGG	-	chr3.1:77993149-77993168	MS.gene06298:CDS	25.0%
	GGTATTTGATTATGATGAAG+TGG	+	chr3.1:77992985-77993004	None:intergenic	30.0%
	TTGATTGCTTTATACACAAG+AGG	+	chr3.1:77992871-77992890	None:intergenic	30.0%
!!	CAGTATGAGGTTATTTCTTT+AGG	-	chr3.1:77992910-77992929	MS.gene06298:CDS	30.0%
!!	GTTGGAGATAATTTTGGTAA+TGG	-	chr3.1:77993048-77993067	MS.gene06298:CDS	30.0%
!!	TATGAGGTTATTTCTTTAGG+AGG	-	chr3.1:77992913-77992932	MS.gene06298:CDS	30.0%
	TTTCATGATCAGACAACAAC+TGG	-	chr3.1:77993078-77993097	MS.gene06298:CDS	35.0%
	AGGAAGCTACAACATAAGCA+TGG	-	chr3.1:77992933-77992952	MS.gene06298:CDS	40.0%
	CACAGGAGACATAAATCTGT+TGG	-	chr3.1:77993030-77993049	MS.gene06298:CDS	40.0%
	GAAGTGGTTGTTGAGTTTGA+GGG	+	chr3.1:77992969-77992988	None:intergenic	40.0%
	TAGAACAAGACGACAGTATG+AGG	-	chr3.1:77992897-77992916	MS.gene06298:CDS	40.0%
	TGAAGTGGTTGTTGAGTTTG+AGG	+	chr3.1:77992970-77992989	None:intergenic	40.0%
	GTGGTTGTTGAGTTTGAGGG+TGG	+	chr3.1:77992966-77992985	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	77992853	77993179	77992853	ID=MS.gene06298
chr3.1	mRNA	77992853	77993179	77992853	ID=MS.gene06298.t1;Parent=MS.gene06298
chr3.1	exon	77992853	77993179	77992853	ID=MS.gene06298.t1.exon1;Parent=MS.gene06298.t1
chr3.1	CDS	77992853	77993179	77992853	ID=cds.MS.gene06298.t1;Parent=MS.gene06298.t1

Gene Sequence	>MS.gene06298 ATGGAAGGTCTTCTACCTCTTGTGTATAAAGCAATCAAGAAAAATAGAACAAGACGACAGTATGAGGTTATTTCTTTAGGAGGAAGCTACAACATAAGCATGGCTGAGATCCACCCTCAAACTCAACAACCACTTCATCATAATCAAATACCAGCTTCAGATAATTACAATATTGGTCACAGGAGACATAAATCTGTTGGAGATAATTTTGGTAATGGTTTTCAATTTCATGATCAGACAACAACTGGTTCTGTTTCTCCTAATTCTTCAAAAAAACTTGTCAGGTTTAGTAGTCATAGAATGTTTTCATGTATCAAGGGTGCCTGA
Protein sequence	>MS.gene06298.t1 MEGLLPLVYKAIKKNRTRRQYEVISLGGSYNISMAEIHPQTQQPLHHNQIPASDNYNIGHRRHKSVGDNFGNGFQFHDQTTTGSVSPNSSKKLVRFSSHRMFSCIKGA