Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063427.t1 | XP_013464343.1 | 95.4 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 9.10E-120 | 439.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063427.t1 | P46417 | 80.6 | 216 | 42 | 0 | 1 | 216 | 1 | 216 | 6.7e-103 | 374.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063427.t1 | B7FGX5 | 95.4 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 6.6e-120 | 439.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene063427 | 0.836102 | 1.14E-56 | -1.69E-46 |
| MS.gene050227 | MS.gene063427 | 0.800043 | 1.73E-48 | -1.69E-46 |
| MS.gene050426 | MS.gene063427 | -0.803071 | 4.15E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene063427 | 0.805246 | 1.46E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene063427 | 0.829304 | 5.55E-55 | -1.69E-46 |
| MS.gene051983 | MS.gene063427 | 0.815648 | 8.30E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene063427 | -0.843148 | 1.67E-58 | -1.69E-46 |
| MS.gene052077 | MS.gene063427 | 0.85417 | 1.47E-61 | -1.69E-46 |
| MS.gene052253 | MS.gene063427 | 0.822277 | 2.58E-53 | -1.69E-46 |
| MS.gene052389 | MS.gene063427 | 0.828257 | 9.94E-55 | -1.69E-46 |
| MS.gene05316 | MS.gene063427 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene053494 | MS.gene063427 | 0.816061 | 6.71E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene063427 | 0.802825 | 4.67E-49 | -1.69E-46 |
| MS.gene054012 | MS.gene063427 | 0.803608 | 3.21E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene063427 | 0.841722 | 4.00E-58 | -1.69E-46 |
| MS.gene054349 | MS.gene063427 | 0.810733 | 9.95E-51 | -1.69E-46 |
| MS.gene054556 | MS.gene063427 | 0.848376 | 6.38E-60 | -1.69E-46 |
| MS.gene055369 | MS.gene063427 | 0.800073 | 1.71E-48 | -1.69E-46 |
| MS.gene05553 | MS.gene063427 | 0.807767 | 4.30E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene063427 | 0.832621 | 8.51E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene063427 | 0.801103 | 1.05E-48 | -1.69E-46 |
| MS.gene056979 | MS.gene063427 | 0.810947 | 8.95E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene063427 | 0.853114 | 2.96E-61 | -1.69E-46 |
| MS.gene057610 | MS.gene063427 | 0.813455 | 2.54E-51 | -1.69E-46 |
| MS.gene059082 | MS.gene063427 | 0.812446 | 4.22E-51 | -1.69E-46 |
| MS.gene059247 | MS.gene063427 | 0.804372 | 2.23E-49 | -1.69E-46 |
| MS.gene059420 | MS.gene063427 | 0.84023 | 9.85E-58 | -1.69E-46 |
| MS.gene059462 | MS.gene063427 | 0.810422 | 1.16E-50 | -1.69E-46 |
| MS.gene059807 | MS.gene063427 | 0.807148 | 5.82E-50 | -1.69E-46 |
| MS.gene059876 | MS.gene063427 | 0.850408 | 1.73E-60 | -1.69E-46 |
| MS.gene061154 | MS.gene063427 | 0.8199 | 9.11E-53 | -1.69E-46 |
| MS.gene061159 | MS.gene063427 | 0.808133 | 3.60E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063427.t1 | MTR_2g070070 | 95.434 | 219 | 10 | 0 | 1 | 219 | 1 | 219 | 7.19e-148 | 410 |
| MS.gene063427.t1 | MTR_2g070060 | 89.041 | 219 | 24 | 0 | 1 | 219 | 1 | 219 | 5.66e-147 | 408 |
| MS.gene063427.t1 | MTR_2g070110 | 88.584 | 219 | 24 | 1 | 1 | 219 | 1 | 218 | 1.70e-135 | 379 |
| MS.gene063427.t1 | MTR_2g070180 | 65.888 | 214 | 73 | 0 | 1 | 214 | 1 | 214 | 3.73e-110 | 315 |
| MS.gene063427.t1 | MTR_2g070130 | 62.557 | 219 | 82 | 0 | 1 | 219 | 1 | 219 | 2.70e-107 | 308 |
| MS.gene063427.t1 | MTR_2g070150 | 67.647 | 204 | 66 | 0 | 1 | 204 | 1 | 204 | 4.31e-107 | 306 |
| MS.gene063427.t1 | MTR_2g070140 | 65.517 | 203 | 70 | 0 | 17 | 219 | 1 | 203 | 8.63e-105 | 301 |
| MS.gene063427.t1 | MTR_8g056940 | 65.297 | 219 | 76 | 0 | 1 | 219 | 1 | 219 | 1.03e-104 | 301 |
| MS.gene063427.t1 | MTR_2g070210 | 61.538 | 221 | 83 | 1 | 1 | 219 | 1 | 221 | 7.74e-103 | 296 |
| MS.gene063427.t1 | MTR_3g467420 | 60.829 | 217 | 85 | 0 | 3 | 219 | 8 | 224 | 4.00e-101 | 292 |
| MS.gene063427.t1 | MTR_2g070200 | 60.909 | 220 | 84 | 2 | 1 | 218 | 1 | 220 | 1.00e-98 | 286 |
| MS.gene063427.t1 | MTR_3g467430 | 60.369 | 217 | 86 | 0 | 3 | 219 | 8 | 224 | 7.78e-98 | 284 |
| MS.gene063427.t1 | MTR_4g124130 | 61.364 | 220 | 84 | 1 | 1 | 219 | 1 | 220 | 1.68e-97 | 283 |
| MS.gene063427.t1 | MTR_2g070120 | 57.416 | 209 | 89 | 0 | 1 | 209 | 1 | 209 | 5.96e-88 | 259 |
| MS.gene063427.t1 | MTR_5g076900 | 52.968 | 219 | 101 | 2 | 2 | 219 | 4 | 221 | 5.57e-78 | 233 |
| MS.gene063427.t1 | MTR_0186s0030 | 66.667 | 141 | 47 | 0 | 1 | 141 | 1 | 141 | 3.03e-64 | 196 |
| MS.gene063427.t1 | MTR_5g037380 | 40.807 | 223 | 123 | 3 | 3 | 216 | 4 | 226 | 3.17e-50 | 163 |
| MS.gene063427.t1 | MTR_5g040430 | 36.323 | 223 | 132 | 3 | 2 | 217 | 5 | 224 | 1.04e-47 | 156 |
| MS.gene063427.t1 | MTR_8g087425 | 41.429 | 210 | 116 | 5 | 2 | 206 | 3 | 210 | 9.84e-46 | 151 |
| MS.gene063427.t1 | MTR_1g090060 | 41.509 | 212 | 115 | 4 | 4 | 209 | 3 | 211 | 1.98e-45 | 150 |
| MS.gene063427.t1 | MTR_1g090070 | 41.038 | 212 | 117 | 5 | 4 | 209 | 9 | 218 | 6.85e-43 | 144 |
| MS.gene063427.t1 | MTR_4g019780 | 37.500 | 208 | 125 | 4 | 4 | 208 | 8 | 213 | 1.01e-42 | 144 |
| MS.gene063427.t1 | MTR_4g019790 | 41.232 | 211 | 119 | 4 | 4 | 211 | 7 | 215 | 1.06e-42 | 144 |
| MS.gene063427.t1 | MTR_2g072120 | 88.732 | 71 | 8 | 0 | 47 | 117 | 1 | 71 | 2.06e-42 | 138 |
| MS.gene063427.t1 | MTR_8g087410 | 40.094 | 212 | 119 | 6 | 1 | 206 | 2 | 211 | 1.30e-41 | 141 |
| MS.gene063427.t1 | MTR_7g065630 | 36.866 | 217 | 128 | 5 | 4 | 217 | 25 | 235 | 5.64e-41 | 140 |
| MS.gene063427.t1 | MTR_8g061950 | 37.778 | 225 | 127 | 5 | 3 | 218 | 4 | 224 | 8.96e-41 | 139 |
| MS.gene063427.t1 | MTR_7g065660 | 39.614 | 207 | 120 | 4 | 5 | 208 | 8 | 212 | 1.07e-40 | 139 |
| MS.gene063427.t1 | MTR_7g065750 | 40.796 | 201 | 113 | 4 | 4 | 200 | 7 | 205 | 1.12e-40 | 139 |
| MS.gene063427.t1 | MTR_4g059730 | 39.269 | 219 | 122 | 6 | 1 | 212 | 1 | 215 | 2.78e-40 | 137 |
| MS.gene063427.t1 | MTR_7g065720 | 37.799 | 209 | 124 | 4 | 4 | 208 | 7 | 213 | 5.18e-40 | 137 |
| MS.gene063427.t1 | MTR_6g080440 | 37.321 | 209 | 126 | 4 | 3 | 208 | 2 | 208 | 8.50e-40 | 136 |
| MS.gene063427.t1 | MTR_1g090100 | 41.584 | 202 | 113 | 3 | 11 | 208 | 9 | 209 | 9.67e-40 | 136 |
| MS.gene063427.t1 | MTR_1g090090 | 40.670 | 209 | 119 | 4 | 4 | 208 | 3 | 210 | 2.84e-39 | 135 |
| MS.gene063427.t1 | MTR_7g065740 | 37.799 | 209 | 124 | 4 | 4 | 208 | 7 | 213 | 6.20e-39 | 134 |
| MS.gene063427.t1 | MTR_7g065710 | 36.493 | 211 | 125 | 5 | 1 | 208 | 1 | 205 | 7.40e-38 | 131 |
| MS.gene063427.t1 | MTR_3g099757 | 37.500 | 216 | 132 | 3 | 3 | 216 | 4 | 218 | 9.38e-38 | 131 |
| MS.gene063427.t1 | MTR_7g065590 | 36.574 | 216 | 132 | 4 | 4 | 216 | 7 | 220 | 1.82e-37 | 130 |
| MS.gene063427.t1 | MTR_1g090150 | 36.486 | 222 | 125 | 6 | 4 | 216 | 3 | 217 | 1.62e-36 | 128 |
| MS.gene063427.t1 | MTR_7g065230 | 40.000 | 200 | 110 | 5 | 4 | 200 | 7 | 199 | 1.21e-35 | 125 |
| MS.gene063427.t1 | MTR_7g065265 | 37.981 | 208 | 124 | 4 | 4 | 208 | 7 | 212 | 1.22e-35 | 125 |
| MS.gene063427.t1 | MTR_7g065270 | 38.500 | 200 | 118 | 4 | 4 | 200 | 7 | 204 | 1.53e-35 | 125 |
| MS.gene063427.t1 | MTR_7g065680 | 35.484 | 217 | 131 | 5 | 4 | 217 | 7 | 217 | 3.42e-35 | 124 |
| MS.gene063427.t1 | MTR_7g065600 | 38.191 | 199 | 118 | 4 | 5 | 200 | 8 | 204 | 1.99e-34 | 122 |
| MS.gene063427.t1 | MTR_7g065290 | 37.500 | 208 | 125 | 4 | 4 | 208 | 7 | 212 | 5.74e-34 | 121 |
| MS.gene063427.t1 | MTR_7g065700 | 36.842 | 209 | 127 | 5 | 1 | 208 | 4 | 208 | 2.82e-33 | 119 |
| MS.gene063427.t1 | MTR_7g065640 | 36.275 | 204 | 113 | 4 | 5 | 208 | 8 | 194 | 5.28e-33 | 118 |
| MS.gene063427.t1 | MTR_1g115195 | 35.885 | 209 | 124 | 5 | 4 | 205 | 3 | 208 | 6.11e-33 | 118 |
| MS.gene063427.t1 | MTR_7g065260 | 36.585 | 205 | 125 | 4 | 4 | 205 | 14 | 216 | 1.41e-29 | 110 |
| MS.gene063427.t1 | MTR_5g037390 | 42.045 | 88 | 49 | 1 | 28 | 113 | 11 | 98 | 4.22e-14 | 66.2 |
| MS.gene063427.t1 | MTR_4g043640 | 65.000 | 40 | 14 | 0 | 150 | 189 | 46 | 85 | 9.56e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene063427.t1 | AT1G17180 | 64.840 | 219 | 77 | 0 | 1 | 219 | 1 | 219 | 8.46e-110 | 314 |
| MS.gene063427.t1 | AT1G78380 | 67.757 | 214 | 69 | 0 | 1 | 214 | 1 | 214 | 3.07e-109 | 313 |
| MS.gene063427.t1 | AT1G78370 | 63.256 | 215 | 79 | 0 | 1 | 215 | 1 | 215 | 1.25e-102 | 296 |
| MS.gene063427.t1 | AT1G78360 | 63.636 | 220 | 79 | 1 | 1 | 219 | 1 | 220 | 1.12e-101 | 293 |
| MS.gene063427.t1 | AT1G17170 | 63.014 | 219 | 80 | 1 | 1 | 219 | 1 | 218 | 2.04e-101 | 293 |
| MS.gene063427.t1 | AT1G78340 | 61.682 | 214 | 82 | 0 | 1 | 214 | 1 | 214 | 1.44e-99 | 288 |
| MS.gene063427.t1 | AT1G78320 | 61.364 | 220 | 84 | 1 | 1 | 219 | 1 | 220 | 3.42e-96 | 280 |
| MS.gene063427.t1 | AT1G17190 | 60.092 | 218 | 86 | 1 | 1 | 217 | 1 | 218 | 6.79e-95 | 276 |
| MS.gene063427.t1 | AT1G53680 | 56.164 | 219 | 94 | 2 | 3 | 219 | 6 | 224 | 3.05e-90 | 265 |
| MS.gene063427.t1 | AT3G43800 | 53.704 | 216 | 96 | 3 | 4 | 215 | 5 | 220 | 5.61e-82 | 244 |
| MS.gene063427.t1 | AT1G78320 | 53.182 | 220 | 75 | 2 | 1 | 219 | 1 | 193 | 3.24e-76 | 228 |
| MS.gene063427.t1 | AT1G10360 | 42.661 | 218 | 116 | 5 | 1 | 209 | 1 | 218 | 6.40e-53 | 170 |
| MS.gene063427.t1 | AT1G59700 | 44.495 | 218 | 106 | 5 | 4 | 209 | 6 | 220 | 1.01e-50 | 164 |
| MS.gene063427.t1 | AT1G59670 | 45.872 | 218 | 103 | 5 | 4 | 209 | 6 | 220 | 4.95e-49 | 160 |
| MS.gene063427.t1 | AT2G29420 | 41.379 | 203 | 116 | 3 | 2 | 202 | 7 | 208 | 1.60e-47 | 156 |
| MS.gene063427.t1 | AT3G09270 | 40.758 | 211 | 122 | 2 | 5 | 212 | 7 | 217 | 1.60e-47 | 156 |
| MS.gene063427.t1 | AT1G10370 | 41.096 | 219 | 115 | 5 | 2 | 209 | 3 | 218 | 3.34e-47 | 155 |
| MS.gene063427.t1 | AT1G74590 | 41.969 | 193 | 106 | 3 | 3 | 189 | 6 | 198 | 9.65e-46 | 152 |
| MS.gene063427.t1 | AT1G69930 | 39.151 | 212 | 122 | 3 | 5 | 209 | 13 | 224 | 3.17e-42 | 143 |
| MS.gene063427.t1 | AT2G29490 | 37.674 | 215 | 130 | 2 | 5 | 216 | 8 | 221 | 6.78e-42 | 142 |
| MS.gene063427.t1 | AT1G27140 | 39.631 | 217 | 121 | 4 | 3 | 209 | 5 | 221 | 3.37e-41 | 140 |
| MS.gene063427.t1 | AT1G27130 | 38.356 | 219 | 120 | 5 | 3 | 209 | 5 | 220 | 7.16e-41 | 139 |
| MS.gene063427.t1 | AT2G29450 | 40.097 | 207 | 111 | 6 | 4 | 202 | 6 | 207 | 1.32e-39 | 135 |
| MS.gene063427.t1 | AT2G29480 | 40.976 | 205 | 113 | 6 | 5 | 204 | 8 | 209 | 1.74e-39 | 135 |
| MS.gene063427.t1 | AT2G29470 | 38.537 | 205 | 119 | 5 | 5 | 204 | 8 | 210 | 2.13e-39 | 135 |
| MS.gene063427.t1 | AT5G62480 | 34.222 | 225 | 136 | 5 | 1 | 214 | 5 | 228 | 2.43e-39 | 135 |
| MS.gene063427.t1 | AT2G29440 | 38.462 | 221 | 122 | 6 | 4 | 216 | 6 | 220 | 2.56e-39 | 135 |
| MS.gene063427.t1 | AT2G29460 | 40.594 | 202 | 112 | 6 | 4 | 200 | 7 | 205 | 4.85e-39 | 134 |
| MS.gene063427.t1 | AT1G69920 | 36.574 | 216 | 124 | 5 | 5 | 209 | 35 | 248 | 8.78e-38 | 132 |
| MS.gene063427.t1 | AT5G62480 | 31.694 | 183 | 90 | 4 | 1 | 175 | 5 | 160 | 1.28e-22 | 91.7 |
| MS.gene063427.t1 | AT5G62480 | 31.694 | 183 | 91 | 4 | 1 | 175 | 5 | 161 | 1.60e-22 | 91.3 |
Find 43 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGCTCAAGCTAGATTT+TGG | 0.158333 | 2.3:-25828378 | MS.gene063427:CDS |
| AGCATAAAACCAGCTGTAAA+AGG | 0.226180 | 2.3:+25827600 | None:intergenic |
| GGTTGATTATATTGACAAAA+AGG | 0.226748 | 2.3:-25828356 | MS.gene063427:intron |
| ATTCCAGTTCTTATTCATAA+TGG | 0.270694 | 2.3:-25828490 | MS.gene063427:CDS |
| CATGAAGAAAACACTTGGTT+TGG | 0.291507 | 2.3:-25827443 | MS.gene063427:CDS |
| ATAATTGCAGATTCACAAAT+AGG | 0.302069 | 2.3:+25828463 | None:intergenic |
| TGCAGTATATAGATGAAGTT+TGG | 0.305831 | 2.3:-25828438 | MS.gene063427:CDS |
| GACATATGGTAACTTTAAAA+TGG | 0.308392 | 2.3:-25827572 | MS.gene063427:CDS |
| AAGACACTAGAGGAAACTTT+AGG | 0.341946 | 2.3:-25827679 | MS.gene063427:CDS |
| GCATGTTTGGTATGAGGGTT+AGG | 0.343737 | 2.3:-25828612 | MS.gene063427:CDS |
| CTCTTTCTCCAAACATCTCT+TGG | 0.346478 | 2.3:+25827519 | None:intergenic |
| AAAGTGGCACCACCGTAAAA+AGG | 0.374250 | 2.3:+25827649 | None:intergenic |
| GATACATGGCCTAGCATGTT+TGG | 0.374420 | 2.3:-25828625 | MS.gene063427:CDS |
| GGCCTAGCATGTTTGGTATG+AGG | 0.389766 | 2.3:-25828618 | MS.gene063427:CDS |
| CTAGCTTGAGCTCTCTCATA+AGG | 0.392919 | 2.3:+25828385 | None:intergenic |
| TTTCCATTATGAATAAGAAC+TGG | 0.412827 | 2.3:+25828487 | None:intergenic |
| TCATACACCTTCATTTGATC+AGG | 0.418180 | 2.3:+25827478 | None:intergenic |
| GCATAAAACCAGCTGTAAAA+GGG | 0.445528 | 2.3:+25827601 | None:intergenic |
| AGGAAAATGTGGCTTTCAAA+AGG | 0.453535 | 2.3:-25827745 | MS.gene063427:CDS |
| TTGGCCCATGCCATCAGCTT+AGG | 0.461650 | 2.3:+25827538 | None:intergenic |
| GCCTAGCATGTTTGGTATGA+GGG | 0.468751 | 2.3:-25828617 | MS.gene063427:CDS |
| GGCATGGGCCAAGAGATGTT+TGG | 0.489429 | 2.3:-25827527 | MS.gene063427:CDS |
| GAATGTAGGTGTATGATACT+TGG | 0.493096 | 2.3:-25827768 | MS.gene063427:intron |
| AACTCTTCCTGATCAAATGA+AGG | 0.495473 | 2.3:-25827485 | MS.gene063427:CDS |
| TGGTAACTTTAAAATGGAAG+TGG | 0.524339 | 2.3:-25827566 | MS.gene063427:CDS |
| TAGCTTGAGCTCTCTCATAA+GGG | 0.529940 | 2.3:+25828386 | None:intergenic |
| GGATTCATCTGAAGAAGTAA+AGG | 0.531810 | 2.3:+25828526 | None:intergenic |
| AATATCAAGAAAGCCAAAAG+TGG | 0.548628 | 2.3:+25827633 | None:intergenic |
| GTGTCCTAAGCTGATGGCAT+GGG | 0.573537 | 2.3:-25827542 | MS.gene063427:CDS |
| AAAGGTGAAGAGCATGAGGA+AGG | 0.578570 | 2.3:-25827727 | MS.gene063427:CDS |
| AAGGTGAAGAGCATGAGGAA+GGG | 0.585110 | 2.3:-25827726 | MS.gene063427:CDS |
| AGTGTCCTAAGCTGATGGCA+TGG | 0.588846 | 2.3:-25827543 | MS.gene063427:CDS |
| CATAAAACCAGCTGTAAAAG+GGG | 0.600460 | 2.3:+25827602 | None:intergenic |
| GCTCTCTCATAAGGGTGAGA+AGG | 0.602075 | 2.3:+25828394 | None:intergenic |
| TACAATCATGGCAAACGAGG+TGG | 0.606147 | 2.3:-25828656 | None:intergenic |
| GTGAGCATGAAGAAAACACT+TGG | 0.623270 | 2.3:-25827448 | MS.gene063427:CDS |
| CTCTATCTTTAAGACACTAG+AGG | 0.627375 | 2.3:-25827689 | MS.gene063427:CDS |
| TTCAAAAGGTGAAGAGCATG+AGG | 0.635141 | 2.3:-25827731 | MS.gene063427:CDS |
| ATGATACTTGGAGGAAAATG+TGG | 0.639144 | 2.3:-25827756 | MS.gene063427:CDS |
| ACCCTCATACCAAACATGCT+AGG | 0.644205 | 2.3:+25828616 | None:intergenic |
| TTCTACAATCATGGCAAACG+AGG | 0.652977 | 2.3:-25828659 | None:intergenic |
| AGTGGAGTGTCCTAAGCTGA+TGG | 0.664603 | 2.3:-25827548 | MS.gene063427:CDS |
| TGTAGGTGTATGATACTTGG+AGG | 0.669059 | 2.3:-25827765 | MS.gene063427:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTTTCATCCTTAATTT+TGG | - | chr2.3:25827776-25827795 | MS.gene063427:intron | 15.0% |
| !! | TAAGATCAAGAATGATTATT+TGG | + | chr2.3:25828264-25828283 | None:intergenic | 20.0% |
| !! | TGGATAAACAATTTACTTAA+AGG | + | chr2.3:25828244-25828263 | None:intergenic | 20.0% |
| !!! | TCATTATTGATTTTGAATGT+AGG | - | chr2.3:25828305-25828324 | MS.gene063427:intron | 20.0% |
| ! | AAAATTAGCAGTGTTTATCA+TGG | + | chr2.3:25827856-25827875 | None:intergenic | 25.0% |
| ! | ATAATTGCAGATTCACAAAT+AGG | + | chr2.3:25827627-25827646 | None:intergenic | 25.0% |
| ! | ATAGCACAAAAATTACCAAA+AGG | + | chr2.3:25827896-25827915 | None:intergenic | 25.0% |
| ! | ATTCCAGTTCTTATTCATAA+TGG | - | chr2.3:25827597-25827616 | MS.gene063427:CDS | 25.0% |
| ! | GGTTGATTATATTGACAAAA+AGG | - | chr2.3:25827731-25827750 | MS.gene063427:CDS | 25.0% |
| ! | TTTCCATTATGAATAAGAAC+TGG | + | chr2.3:25827603-25827622 | None:intergenic | 25.0% |
| !! | ACTGGAATTTTCTTATGAAT+TGG | + | chr2.3:25827585-25827604 | None:intergenic | 25.0% |
| !! | ATAAACACTGCTAATTTTTG+TGG | - | chr2.3:25827858-25827877 | MS.gene063427:intron | 25.0% |
| !! | ATTTTCATCCTTAATTTTGG+TGG | - | chr2.3:25827779-25827798 | MS.gene063427:intron | 25.0% |
| !! | GACATATGGTAACTTTAAAA+TGG | - | chr2.3:25828515-25828534 | MS.gene063427:CDS | 25.0% |
| !! | TCTAGACTTTTGAATGTATT+TGG | + | chr2.3:25828101-25828120 | None:intergenic | 25.0% |
| !! | TTTTATGCTTATGAGACATA+TGG | - | chr2.3:25828501-25828520 | MS.gene063427:CDS | 25.0% |
| !!! | TGAGAGGATTAACAATTTTT+TGG | + | chr2.3:25828057-25828076 | None:intergenic | 25.0% |
| !!! | TTTTCATCCTTAATTTTGGT+GGG | - | chr2.3:25827780-25827799 | MS.gene063427:intron | 25.0% |
| AATATCAAGAAAGCCAAAAG+TGG | + | chr2.3:25828457-25828476 | None:intergenic | 30.0% | |
| AATTGTTAATCCTCTCATGA+CGG | - | chr2.3:25828060-25828079 | MS.gene063427:intron | 30.0% | |
| ATCAAAACCCACCAAAATTA+AGG | + | chr2.3:25827790-25827809 | None:intergenic | 30.0% | |
| TGCAGTATATAGATGAAGTT+TGG | - | chr2.3:25827649-25827668 | MS.gene063427:CDS | 30.0% | |
| ! | TTTGAATGTATTTGGAGCTT+AGG | + | chr2.3:25828093-25828112 | None:intergenic | 30.0% |
| !! | TGGTAACTTTAAAATGGAAG+TGG | - | chr2.3:25828521-25828540 | MS.gene063427:CDS | 30.0% |
| !! | TGGTAGATATTTTTGCCTTT+TGG | - | chr2.3:25827878-25827897 | MS.gene063427:intron | 30.0% |
| !!! | ACTTTTGGCTTTCTTGATAT+TGG | - | chr2.3:25828456-25828475 | MS.gene063427:CDS | 30.0% |
| AACTCTTCCTGATCAAATGA+AGG | - | chr2.3:25828602-25828621 | MS.gene063427:CDS | 35.0% | |
| AAGACACTAGAGGAAACTTT+AGG | - | chr2.3:25828408-25828427 | MS.gene063427:CDS | 35.0% | |
| AGCATAAAACCAGCTGTAAA+AGG | + | chr2.3:25828490-25828509 | None:intergenic | 35.0% | |
| AGGAAAATGTGGCTTTCAAA+AGG | - | chr2.3:25828342-25828361 | MS.gene063427:intron | 35.0% | |
| ATGATACTTGGAGGAAAATG+TGG | - | chr2.3:25828331-25828350 | MS.gene063427:intron | 35.0% | |
| CATAAAACCAGCTGTAAAAG+GGG | + | chr2.3:25828488-25828507 | None:intergenic | 35.0% | |
| CATGAAGAAAACACTTGGTT+TGG | - | chr2.3:25828644-25828663 | MS.gene063427:CDS | 35.0% | |
| GAATGTAGGTGTATGATACT+TGG | - | chr2.3:25828319-25828338 | MS.gene063427:intron | 35.0% | |
| GCATAAAACCAGCTGTAAAA+GGG | + | chr2.3:25828489-25828508 | None:intergenic | 35.0% | |
| GGATTCATCTGAAGAAGTAA+AGG | + | chr2.3:25827564-25827583 | None:intergenic | 35.0% | |
| TCATACACCTTCATTTGATC+AGG | + | chr2.3:25828612-25828631 | None:intergenic | 35.0% | |
| ! | TTAGGTGACAAGCCTTTTTA+CGG | - | chr2.3:25828426-25828445 | MS.gene063427:CDS | 35.0% |
| !! | CTCTATCTTTAAGACACTAG+AGG | - | chr2.3:25828398-25828417 | MS.gene063427:CDS | 35.0% |
| CTCTTTCTCCAAACATCTCT+TGG | + | chr2.3:25828571-25828590 | None:intergenic | 40.0% | |
| GTGAGCATGAAGAAAACACT+TGG | - | chr2.3:25828639-25828658 | MS.gene063427:CDS | 40.0% | |
| TAGCTTGAGCTCTCTCATAA+GGG | + | chr2.3:25827704-25827723 | None:intergenic | 40.0% | |
| TGTAGGTGTATGATACTTGG+AGG | - | chr2.3:25828322-25828341 | MS.gene063427:intron | 40.0% | |
| ! | AGAGAGCTCAAGCTAGATTT+TGG | - | chr2.3:25827709-25827728 | MS.gene063427:CDS | 40.0% |
| ! | GAGAGCTCAAGCTAGATTTT+GGG | - | chr2.3:25827710-25827729 | MS.gene063427:CDS | 40.0% |
| ! | TTCAAAAGGTGAAGAGCATG+AGG | - | chr2.3:25828356-25828375 | MS.gene063427:intron | 40.0% |
| !!! | AGGTGGTTTTGTTGGATACA+TGG | - | chr2.3:25827448-25827467 | MS.gene063427:CDS | 40.0% |
| AAAGTGGCACCACCGTAAAA+AGG | + | chr2.3:25828441-25828460 | None:intergenic | 45.0% | |
| ACCCTCATACCAAACATGCT+AGG | + | chr2.3:25827474-25827493 | None:intergenic | 45.0% | |
| CTAGCTTGAGCTCTCTCATA+AGG | + | chr2.3:25827705-25827724 | None:intergenic | 45.0% | |
| GATACATGGCCTAGCATGTT+TGG | - | chr2.3:25827462-25827481 | MS.gene063427:CDS | 45.0% | |
| GCATGTTTGGTATGAGGGTT+AGG | - | chr2.3:25827475-25827494 | MS.gene063427:CDS | 45.0% | |
| GCCTAGCATGTTTGGTATGA+GGG | - | chr2.3:25827470-25827489 | MS.gene063427:CDS | 45.0% | |
| ! | AAAGGTGAAGAGCATGAGGA+AGG | - | chr2.3:25828360-25828379 | MS.gene063427:CDS | 45.0% |
| ! | AAGGTGAAGAGCATGAGGAA+GGG | - | chr2.3:25828361-25828380 | MS.gene063427:CDS | 45.0% |
| !! | GTTGTCTCAGAAAGTGTCAC+AGG | + | chr2.3:25827987-25828006 | None:intergenic | 45.0% |
| !!! | TTTTACGGTGGTGCCACTTT+TGG | - | chr2.3:25828441-25828460 | MS.gene063427:CDS | 45.0% |
| AGGTAGTTGTCCGTCATGAG+AGG | + | chr2.3:25828073-25828092 | None:intergenic | 50.0% | |
| AGTGTCCTAAGCTGATGGCA+TGG | - | chr2.3:25828544-25828563 | MS.gene063427:CDS | 50.0% | |
| GCTCTCTCATAAGGGTGAGA+AGG | + | chr2.3:25827696-25827715 | None:intergenic | 50.0% | |
| GGCCTAGCATGTTTGGTATG+AGG | - | chr2.3:25827469-25827488 | MS.gene063427:CDS | 50.0% | |
| GTGTCCTAAGCTGATGGCAT+GGG | - | chr2.3:25828545-25828564 | MS.gene063427:CDS | 50.0% | |
| ! | AGTGGAGTGTCCTAAGCTGA+TGG | - | chr2.3:25828539-25828558 | MS.gene063427:CDS | 50.0% |
| ! | GACTGATCCCCTTTTACAGC+TGG | - | chr2.3:25828478-25828497 | MS.gene063427:CDS | 50.0% |
| ! | GGTGACAAGCCTTTTTACGG+TGG | - | chr2.3:25828429-25828448 | MS.gene063427:CDS | 50.0% |
| !!! | GGCAAACGAGGTGGTTTTGT+TGG | - | chr2.3:25827440-25827459 | MS.gene063427:CDS | 50.0% |
| GGCATGGGCCAAGAGATGTT+TGG | - | chr2.3:25828560-25828579 | MS.gene063427:CDS | 55.0% | |
| TTGGCCCATGCCATCAGCTT+AGG | + | chr2.3:25828552-25828571 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 25827438 | 25828671 | 25827438 | ID=MS.gene063427 |
| chr2.3 | mRNA | 25827438 | 25828671 | 25827438 | ID=MS.gene063427.t1;Parent=MS.gene063427 |
| chr2.3 | exon | 25828357 | 25828671 | 25828357 | ID=MS.gene063427.t1.exon1;Parent=MS.gene063427.t1 |
| chr2.3 | CDS | 25828357 | 25828671 | 25828357 | ID=cds.MS.gene063427.t1;Parent=MS.gene063427.t1 |
| chr2.3 | exon | 25827438 | 25827782 | 25827438 | ID=MS.gene063427.t1.exon2;Parent=MS.gene063427.t1 |
| chr2.3 | CDS | 25827438 | 25827782 | 25827438 | ID=cds.MS.gene063427.t1;Parent=MS.gene063427.t1 |
| Gene Sequence |
| Protein sequence |
