Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06352.t1 | KEH19492.1 | 82.1 | 162 | 19 | 4 | 54 | 208 | 42 | 200 | 1.90E-66 | 262.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06352.t1 | A0A072TQ21 | 82.1 | 162 | 19 | 4 | 54 | 208 | 42 | 200 | 1.4e-66 | 262.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77468 | MS.gene06352 | PPI |
| MS.gene71284 | MS.gene06352 | PPI |
| MS.gene77472 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene049263 | PPI |
| MS.gene77466 | MS.gene06352 | PPI |
| MS.gene70476 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene71531 | PPI |
| MS.gene25704 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene044323 | PPI |
| MS.gene06352 | MS.gene71282 | PPI |
| MS.gene044361 | MS.gene06352 | PPI |
| MS.gene79115 | MS.gene06352 | PPI |
| MS.gene044370 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene045214 | PPI |
| MS.gene70443 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene71524 | PPI |
| MS.gene06352 | MS.gene71528 | PPI |
| MS.gene044376 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene70424 | PPI |
| MS.gene044363 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene77464 | PPI |
| MS.gene70430 | MS.gene06352 | PPI |
| MS.gene70418 | MS.gene06352 | PPI |
| MS.gene06352 | MS.gene044326 | PPI |
| MS.gene06352 | MS.gene70427 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06352.t1 | MTR_8g461300 | 82.812 | 192 | 23 | 4 | 24 | 208 | 12 | 200 | 1.24e-111 | 317 |
| MS.gene06352.t1 | MTR_4g055800 | 48.945 | 237 | 75 | 10 | 1 | 206 | 71 | 292 | 1.45e-53 | 173 |
| MS.gene06352.t1 | MTR_3g080680 | 43.030 | 165 | 80 | 5 | 47 | 206 | 43 | 198 | 2.30e-31 | 113 |
| MS.gene06352.t1 | MTR_3g082420 | 39.269 | 219 | 109 | 8 | 8 | 206 | 7 | 221 | 2.76e-29 | 108 |
| MS.gene06352.t1 | MTR_3g082340 | 37.500 | 200 | 113 | 6 | 12 | 206 | 30 | 222 | 1.36e-25 | 99.4 |
| MS.gene06352.t1 | MTR_3g083030 | 36.765 | 204 | 100 | 11 | 15 | 206 | 43 | 229 | 3.37e-19 | 82.8 |
| MS.gene06352.t1 | MTR_3g083050 | 37.222 | 180 | 89 | 9 | 35 | 206 | 43 | 206 | 1.28e-18 | 80.5 |
| MS.gene06352.t1 | MTR_3g083080 | 34.359 | 195 | 105 | 9 | 20 | 206 | 26 | 205 | 2.08e-16 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACACTTTCTTCGCAGAATT+TGG | 0.126426 | 3.1:+77286537 | None:intergenic |
| GATTTCAGCCGTTTCGATTT+CGG | 0.272727 | 3.1:+77286499 | None:intergenic |
| GAATCTTCTGGATCCGATTT+CGG | 0.322438 | 3.1:+77286472 | None:intergenic |
| TCACGACGCTTATACCAAAA+TGG | 0.333746 | 3.1:+77286394 | None:intergenic |
| TCACTATACTCAGAATCTTC+TGG | 0.339930 | 3.1:+77286460 | None:intergenic |
| GCTGAAATCGGAACCGAAAT+CGG | 0.362256 | 3.1:-77286485 | MS.gene06352:CDS |
| TGGTTCTATGTATACGAATA+AGG | 0.367166 | 3.1:-77286371 | MS.gene06352:CDS |
| CTACTTCTTTATATGCTCCT+AGG | 0.380060 | 3.1:-77286027 | MS.gene06352:CDS |
| GAAATCGAAACGGCTGAAAT+CGG | 0.384058 | 3.1:-77286497 | MS.gene06352:CDS |
| ATGACACACTCTTTGACAAC+AGG | 0.418178 | 3.1:+77285896 | None:intergenic |
| CACTGTCCACCAGGTTGTGC+TGG | 0.442305 | 3.1:-77286202 | MS.gene06352:CDS |
| CTGCGAAGAAAGTGTGAAAT+CGG | 0.443953 | 3.1:-77286530 | MS.gene06352:CDS |
| TACGCTGAAGTACATGAGTC+TGG | 0.446308 | 3.1:+77285930 | None:intergenic |
| ACTTTATGAAACACAAAGGT+TGG | 0.454208 | 3.1:+77286073 | None:intergenic |
| CAATCAAAATAATATTAAGC+AGG | 0.465295 | 3.1:-77286098 | MS.gene06352:CDS |
| AGAAAGTGTGAAATCGGAAC+AGG | 0.500922 | 3.1:-77286524 | MS.gene06352:CDS |
| GTTGTCAAGTGTACCCATCC+TGG | 0.532074 | 3.1:-77286052 | MS.gene06352:CDS |
| GTAGTGTTTCAAAATGTCGA+TGG | 0.539331 | 3.1:-77286572 | None:intergenic |
| ATCTTCATCGGTTAAGTCGA+GGG | 0.545269 | 3.1:+77286168 | None:intergenic |
| TACTTCTTTATATGCTCCTA+GGG | 0.547879 | 3.1:-77286026 | MS.gene06352:CDS |
| AACAGGAACCGAAATCGAAA+CGG | 0.558710 | 3.1:-77286507 | MS.gene06352:CDS |
| GATGCTATCCACTGTCCACC+AGG | 0.563532 | 3.1:-77286211 | MS.gene06352:CDS |
| GTATACGAATAAGGCTTGGA+AGG | 0.565787 | 3.1:-77286362 | MS.gene06352:CDS |
| GCATATAAAGAAGTAGCACC+AGG | 0.567371 | 3.1:+77286034 | None:intergenic |
| CTTCAAAGCAGCATTGCAGA+GGG | 0.572155 | 3.1:+77286126 | None:intergenic |
| AAAGTAATGTAATACGCCCT+AGG | 0.574461 | 3.1:+77286010 | None:intergenic |
| TGTCCACCAGGTTGTGCTGG+CGG | 0.578846 | 3.1:-77286199 | MS.gene06352:CDS |
| CTATGTATACGAATAAGGCT+TGG | 0.579383 | 3.1:-77286366 | MS.gene06352:CDS |
| TAAAGAAGTAGCACCAGGAT+GGG | 0.590468 | 3.1:+77286039 | None:intergenic |
| AGTAGGGAGCACATCTTCAT+CGG | 0.590789 | 3.1:+77286156 | None:intergenic |
| AATCAAAATAATATTAAGCA+GGG | 0.591058 | 3.1:-77286097 | MS.gene06352:CDS |
| ACAATACCGCCAGCACAACC+TGG | 0.600366 | 3.1:+77286193 | None:intergenic |
| CATCTTCATCGGTTAAGTCG+AGG | 0.606603 | 3.1:+77286167 | None:intergenic |
| TCTTCAAAGCAGCATTGCAG+AGG | 0.609112 | 3.1:+77286125 | None:intergenic |
| ATAAAGAAGTAGCACCAGGA+TGG | 0.610411 | 3.1:+77286038 | None:intergenic |
| GACAACTTTATGAAACACAA+AGG | 0.610440 | 3.1:+77286069 | None:intergenic |
| AAAGTTGTTAGAGCACGAGC+AGG | 0.617312 | 3.1:+77285953 | None:intergenic |
| CAGCACAACCTGGTGGACAG+TGG | 0.663371 | 3.1:+77286203 | None:intergenic |
| TAAGGCTTGGAAGGAACGTG+AGG | 0.666952 | 3.1:-77286353 | MS.gene06352:CDS |
| TCTTCATCGGTTAAGTCGAG+GGG | 0.670562 | 3.1:+77286169 | None:intergenic |
| TGAGTATAGTGATGAAGACG+AGG | 0.686862 | 3.1:-77286449 | MS.gene06352:CDS |
| ATACCGCCAGCACAACCTGG+TGG | 0.701849 | 3.1:+77286196 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCAAAATAATATTAAGCA+GGG | - | chr3.1:77286345-77286364 | MS.gene06352:CDS | 15.0% |
| !! | CAATCAAAATAATATTAAGC+AGG | - | chr3.1:77286344-77286363 | MS.gene06352:CDS | 20.0% |
| ! | AGCAAAAGCTAAAAAGAAAA+AGG | - | chr3.1:77286458-77286477 | MS.gene06352:CDS | 25.0% |
| ! | GCAAAAGCTAAAAAGAAAAA+GGG | - | chr3.1:77286459-77286478 | MS.gene06352:CDS | 25.0% |
| AAAGCTAAAAAGAAAAAGGG+AGG | - | chr3.1:77286462-77286481 | MS.gene06352:CDS | 30.0% | |
| ACTTTATGAAACACAAAGGT+TGG | + | chr3.1:77286372-77286391 | None:intergenic | 30.0% | |
| GACAACTTTATGAAACACAA+AGG | + | chr3.1:77286376-77286395 | None:intergenic | 30.0% | |
| TACTTCTTTATATGCTCCTA+GGG | - | chr3.1:77286416-77286435 | MS.gene06352:CDS | 30.0% | |
| ! | ACAATGACAGTTTTCCATTT+TGG | - | chr3.1:77286034-77286053 | MS.gene06352:CDS | 30.0% |
| ! | TGGTTCTATGTATACGAATA+AGG | - | chr3.1:77286071-77286090 | MS.gene06352:CDS | 30.0% |
| !!! | CTTAATTTTGATTGATTCGC+AGG | - | chr3.1:77286203-77286222 | MS.gene06352:CDS | 30.0% |
| AAAGTAATGTAATACGCCCT+AGG | + | chr3.1:77286435-77286454 | None:intergenic | 35.0% | |
| CTACTTCTTTATATGCTCCT+AGG | - | chr3.1:77286415-77286434 | MS.gene06352:CDS | 35.0% | |
| CTATGTATACGAATAAGGCT+TGG | - | chr3.1:77286076-77286095 | MS.gene06352:CDS | 35.0% | |
| TCACTATACTCAGAATCTTC+TGG | + | chr3.1:77285985-77286004 | None:intergenic | 35.0% | |
| AACAGGAACCGAAATCGAAA+CGG | - | chr3.1:77285935-77285954 | MS.gene06352:CDS | 40.0% | |
| ATAAAGAAGTAGCACCAGGA+TGG | + | chr3.1:77286407-77286426 | None:intergenic | 40.0% | |
| ATGACACACTCTTTGACAAC+AGG | + | chr3.1:77286549-77286568 | None:intergenic | 40.0% | |
| CACACTTTCTTCGCAGAATT+TGG | + | chr3.1:77285908-77285927 | None:intergenic | 40.0% | |
| GAAATCGAAACGGCTGAAAT+CGG | - | chr3.1:77285945-77285964 | MS.gene06352:CDS | 40.0% | |
| GAATCTTCTGGATCCGATTT+CGG | + | chr3.1:77285973-77285992 | None:intergenic | 40.0% | |
| GATTTCAGCCGTTTCGATTT+CGG | + | chr3.1:77285946-77285965 | None:intergenic | 40.0% | |
| GCATATAAAGAAGTAGCACC+AGG | + | chr3.1:77286411-77286430 | None:intergenic | 40.0% | |
| GTATACGAATAAGGCTTGGA+AGG | - | chr3.1:77286080-77286099 | MS.gene06352:CDS | 40.0% | |
| TAAAGAAGTAGCACCAGGAT+GGG | + | chr3.1:77286406-77286425 | None:intergenic | 40.0% | |
| TCACGACGCTTATACCAAAA+TGG | + | chr3.1:77286051-77286070 | None:intergenic | 40.0% | |
| TGAGTATAGTGATGAAGACG+AGG | - | chr3.1:77285993-77286012 | MS.gene06352:CDS | 40.0% | |
| ! | AGAAAGTGTGAAATCGGAAC+AGG | - | chr3.1:77285918-77285937 | MS.gene06352:CDS | 40.0% |
| ! | ATCTTCATCGGTTAAGTCGA+GGG | + | chr3.1:77286277-77286296 | None:intergenic | 40.0% |
| ! | CTGCGAAGAAAGTGTGAAAT+CGG | - | chr3.1:77285912-77285931 | MS.gene06352:CDS | 40.0% |
| !! | TTTTGGTATAAGCGTCGTGA+TGG | - | chr3.1:77286051-77286070 | MS.gene06352:CDS | 40.0% |
| AAAGTTGTTAGAGCACGAGC+AGG | + | chr3.1:77286492-77286511 | None:intergenic | 45.0% | |
| CTTCAAAGCAGCATTGCAGA+GGG | + | chr3.1:77286319-77286338 | None:intergenic | 45.0% | |
| GCTGAAATCGGAACCGAAAT+CGG | - | chr3.1:77285957-77285976 | MS.gene06352:CDS | 45.0% | |
| TACGCTGAAGTACATGAGTC+TGG | + | chr3.1:77286515-77286534 | None:intergenic | 45.0% | |
| TCTTCAAAGCAGCATTGCAG+AGG | + | chr3.1:77286320-77286339 | None:intergenic | 45.0% | |
| ! | AGTAGGGAGCACATCTTCAT+CGG | + | chr3.1:77286289-77286308 | None:intergenic | 45.0% |
| ! | CATCTTCATCGGTTAAGTCG+AGG | + | chr3.1:77286278-77286297 | None:intergenic | 45.0% |
| ! | TCTTCATCGGTTAAGTCGAG+GGG | + | chr3.1:77286276-77286295 | None:intergenic | 45.0% |
| !!! | TGCAGAGGGTTTTGAGAGTA+GGG | + | chr3.1:77286305-77286324 | None:intergenic | 45.0% |
| !!! | TTGCAGAGGGTTTTGAGAGT+AGG | + | chr3.1:77286306-77286325 | None:intergenic | 45.0% |
| CGCTCTAGCGAACTAAGTGT+AGG | - | chr3.1:77286135-77286154 | MS.gene06352:CDS | 50.0% | |
| GTTGTCAAGTGTACCCATCC+TGG | - | chr3.1:77286390-77286409 | MS.gene06352:CDS | 50.0% | |
| TAAGGCTTGGAAGGAACGTG+AGG | - | chr3.1:77286089-77286108 | MS.gene06352:CDS | 50.0% | |
| ACAATACCGCCAGCACAACC+TGG | + | chr3.1:77286252-77286271 | None:intergenic | 55.0% | |
| GATGCTATCCACTGTCCACC+AGG | - | chr3.1:77286231-77286250 | MS.gene06352:intron | 55.0% | |
| ATACCGCCAGCACAACCTGG+TGG | + | chr3.1:77286249-77286268 | None:intergenic | 60.0% | |
| CACTGTCCACCAGGTTGTGC+TGG | - | chr3.1:77286240-77286259 | MS.gene06352:intron | 60.0% | |
| TGTCCACCAGGTTGTGCTGG+CGG | - | chr3.1:77286243-77286262 | MS.gene06352:intron | 60.0% | |
| ! | CAGCACAACCTGGTGGACAG+TGG | + | chr3.1:77286242-77286261 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 77285883 | 77286581 | 77285883 | ID=MS.gene06352 |
| chr3.1 | mRNA | 77285883 | 77286581 | 77285883 | ID=MS.gene06352.t1;Parent=MS.gene06352 |
| chr3.1 | exon | 77286312 | 77286581 | 77286312 | ID=MS.gene06352.t1.exon1;Parent=MS.gene06352.t1 |
| chr3.1 | CDS | 77286312 | 77286581 | 77286312 | ID=cds.MS.gene06352.t1;Parent=MS.gene06352.t1 |
| chr3.1 | exon | 77285883 | 77286239 | 77285883 | ID=MS.gene06352.t1.exon2;Parent=MS.gene06352.t1 |
| chr3.1 | CDS | 77285883 | 77286239 | 77285883 | ID=cds.MS.gene06352.t1;Parent=MS.gene06352.t1 |
| Gene Sequence |
| Protein sequence |
