AlfalfaGEDB | MS.gene064006

M. sativa cultivar XinJiangDaYe / MS.gene064006

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064006.t1	PNX74769.1	62.7	185	65	2	6	186	10	194	4.30E-54	221.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064006.t1	A0A2K3L8B1	62.7	185	65	2	6	186	10	194	3.1e-54	221.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene064006	MS.gene044323	PPI
MS.gene064006	MS.gene044376	PPI
MS.gene064006	MS.gene70443	PPI
MS.gene064006	MS.gene70430	PPI
MS.gene044363	MS.gene064006	PPI
MS.gene064006	MS.gene71528	PPI
MS.gene064006	MS.gene25704	PPI
MS.gene064006	MS.gene77466	PPI
MS.gene064006	MS.gene77468	PPI
MS.gene064006	MS.gene71531	PPI
MS.gene064006	MS.gene71282	PPI
MS.gene064006	MS.gene71284	PPI
MS.gene064006	MS.gene045214	PPI
MS.gene064006	MS.gene044361	PPI
MS.gene77472	MS.gene064006	PPI
MS.gene064006	MS.gene71524	PPI
MS.gene064006	MS.gene70424	PPI
MS.gene064006	MS.gene77464	PPI
MS.gene064006	MS.gene70418	PPI
MS.gene064006	MS.gene049263	PPI
MS.gene064006	MS.gene044370	PPI
MS.gene064006	MS.gene044326	PPI
MS.gene064006	MS.gene79115	PPI
MS.gene064006	MS.gene70427	PPI
MS.gene064006	MS.gene70476	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene064006.t1	MTR_3g083080	55.676	185	82	0	2	186	23	207	2.24e-67	204
MS.gene064006.t1	MTR_3g083030	53.723	188	82	3	2	186	46	231	3.46e-62	192
MS.gene064006.t1	MTR_3g083050	56.213	169	71	2	19	186	42	208	2.24e-59	184
MS.gene064006.t1	MTR_3g082420	35.385	195	110	6	2	184	31	221	6.89e-28	104
MS.gene064006.t1	MTR_3g082340	36.585	164	91	5	30	184	63	222	8.91e-25	96.7
MS.gene064006.t1	MTR_3g086030	49.505	101	49	1	9	109	5	103	2.56e-24	93.6
MS.gene064006.t1	MTR_3g080680	37.423	163	89	6	30	184	41	198	4.24e-24	94.0
MS.gene064006.t1	MTR_3g083150	41.401	157	84	4	35	184	56	211	4.33e-22	89.4
MS.gene064006.t1	MTR_3g083100	41.600	125	57	4	76	184	49	173	1.07e-14	68.9
MS.gene064006.t1	MTR_4g055800	33.566	143	75	5	34	168	143	273	1.14e-14	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 48 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATACGCAATGAAGAACTTA+TGG	0.249220	5.2:+37239537	MS.gene064006:CDS
GCACTTTGTGTATAGGAATA+AGG	0.280516	5.2:+37239615	MS.gene064006:CDS
CCGCATAAACGCGCAATTGC+TGG	0.285411	5.2:-37239830	None:intergenic
GGCGTGTCCGGTTTCGATTT+TGG	0.286575	5.2:-37239505	None:intergenic
TCCCCTCTGCAAGCTTGCTT+TGG	0.301525	5.2:+37239901	MS.gene064006:CDS
TGCGAACACATAAACATATA+AGG	0.360061	5.2:-37239595	None:intergenic
GAAATACAATGCCGAGAATC+AGG	0.370063	5.2:+37239925	MS.gene064006:CDS
TGTGTTCGCACTTTGTGTAT+AGG	0.382348	5.2:+37239608	MS.gene064006:CDS
ATCGATTCCCACGGCGTGTC+CGG	0.394276	5.2:-37239517	None:intergenic
CATGAGCTAGGTAGGCTTCA+TGG	0.407712	5.2:-37240210	None:intergenic
GATGCTTACAATTAGGTTAC+GGG	0.413589	5.2:-37239695	None:intergenic
AGATGCTTACAATTAGGTTA+CGG	0.428055	5.2:-37239696	None:intergenic
TATTGAACCTACCTGATTCT+CGG	0.452481	5.2:-37239936	None:intergenic
GGCAGCATCATTTGCTTGAA+AGG	0.459047	5.2:-37240105	None:intergenic
TGCAGAGGGGAGTGAGTTCA+AGG	0.476525	5.2:-37239889	None:intergenic
TGGCTCCATCGTCGGTGGTA+AGG	0.478145	5.2:-37239485	None:intergenic
ATACGCAATGAAGAACTTAT+GGG	0.478732	5.2:+37239538	MS.gene064006:CDS
AGATCATCGTCCATGCGATC+AGG	0.496351	5.2:-37239863	None:intergenic
AGCAGGGCAGTTGGAAGGAT+GGG	0.501180	5.2:-37240126	None:intergenic
TCAGCCTGAAAAGTTATAGC+AGG	0.514365	5.2:-37240143	None:intergenic
CCAGCAATTGCGCGTTTATG+CGG	0.515594	5.2:+37239830	MS.gene064006:CDS
CTCCAAAGCAAGCTTGCAGA+GGG	0.518878	5.2:-37239903	None:intergenic
AATACACAATATTACCTCCA+TGG	0.530348	5.2:-37240076	None:intergenic
GATGGAGCCAAAATCGAAAC+CGG	0.533793	5.2:+37239498	MS.gene064006:CDS
TAGCAGGGCAGTTGGAAGGA+TGG	0.540470	5.2:-37240127	None:intergenic
AAAAGTTATAGCAGGGCAGT+TGG	0.545343	5.2:-37240135	None:intergenic
TATTAATGCATGAGCTAGGT+AGG	0.547710	5.2:-37240218	None:intergenic
AATCGAAACCGGACACGCCG+TGG	0.547812	5.2:+37239509	MS.gene064006:CDS
TAAACGCGCAATTGCTGGAG+GGG	0.549997	5.2:-37239825	None:intergenic
ATACACAATATTACCTCCAT+GGG	0.562952	5.2:-37240075	None:intergenic
ATGGCACAAGACTCAACTAC+AGG	0.572178	5.2:-37240191	None:intergenic
CATAAACGCGCAATTGCTGG+AGG	0.575760	5.2:-37239827	None:intergenic
GACATTCTTAAGTCAACCCA+TGG	0.577711	5.2:+37240059	MS.gene064006:CDS
GTTATAGCAGGGCAGTTGGA+AGG	0.582712	5.2:-37240131	None:intergenic
GCGCTTTCTATGCTCAGCCA+TGG	0.587248	5.2:-37239671	None:intergenic
GCAATTGCGCGTTTATGCGG+CGG	0.590566	5.2:+37239833	MS.gene064006:CDS
ATCGAAACCGGACACGCCGT+GGG	0.595257	5.2:+37239510	MS.gene064006:CDS
TGAAGAAATTCGAGTCGCCA+TGG	0.603715	5.2:+37239654	MS.gene064006:CDS
CGGTATCAGACCTGATCGCA+TGG	0.607640	5.2:+37239853	MS.gene064006:CDS
TCTCCAAAGCAAGCTTGCAG+AGG	0.618622	5.2:-37239904	None:intergenic
AGCTACCTTACCACCGACGA+TGG	0.624415	5.2:+37239480	MS.gene064006:CDS
TCATTGCGTATCGATTCCCA+CGG	0.632393	5.2:-37239526	None:intergenic
CAGCCTGAAAAGTTATAGCA+GGG	0.635889	5.2:-37240142	None:intergenic
ATAAACGCGCAATTGCTGGA+GGG	0.641638	5.2:-37239826	None:intergenic
GCACAAGACTCAACTACAGG+CGG	0.689389	5.2:-37240188	None:intergenic
ATTCTTAAGTCAACCCATGG+AGG	0.706999	5.2:+37240062	MS.gene064006:CDS
TCCAAAGCAAGCTTGCAGAG+GGG	0.721977	5.2:-37239902	None:intergenic
AGAGGGGAGTGAGTTCAAGG+CGG	0.771754	5.2:-37239886	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTATTCTTGTTAATTATTA+GGG	+	chr5.2:37240017-37240036	MS.gene064006:intron	15.0%
!!	TAGTATTCTTGTTAATTATT+AGG	+	chr5.2:37240016-37240035	MS.gene064006:intron	15.0%
!!	ATAAATAGATGCTTACAATT+AGG	-	chr5.2:37239705-37239724	None:intergenic	20.0%
!	AATTAAGTGAAACGCAAAAT+TGG	-	chr5.2:37239745-37239764	None:intergenic	25.0%
	AATACACAATATTACCTCCA+TGG	-	chr5.2:37240079-37240098	None:intergenic	30.0%
	AGATGCTTACAATTAGGTTA+CGG	-	chr5.2:37239699-37239718	None:intergenic	30.0%
	ATACACAATATTACCTCCAT+GGG	-	chr5.2:37240078-37240097	None:intergenic	30.0%
	ATACGCAATGAAGAACTTAT+GGG	+	chr5.2:37239538-37239557	MS.gene064006:CDS	30.0%
	TGCGAACACATAAACATATA+AGG	-	chr5.2:37239598-37239617	None:intergenic	30.0%
!!	TAATCTCTTGATTTTGATCC+TGG	+	chr5.2:37239782-37239801	MS.gene064006:intron	30.0%
	GATACGCAATGAAGAACTTA+TGG	+	chr5.2:37239537-37239556	MS.gene064006:CDS	35.0%
	GATGCTTACAATTAGGTTAC+GGG	-	chr5.2:37239698-37239717	None:intergenic	35.0%
	GCACTTTGTGTATAGGAATA+AGG	+	chr5.2:37239615-37239634	MS.gene064006:CDS	35.0%
	TATTGAACCTACCTGATTCT+CGG	-	chr5.2:37239939-37239958	None:intergenic	35.0%
	AAAAGTTATAGCAGGGCAGT+TGG	-	chr5.2:37240138-37240157	None:intergenic	40.0%
	ATAGCATCAAATACCTGTCC+AGG	-	chr5.2:37239803-37239822	None:intergenic	40.0%
	ATTCTTAAGTCAACCCATGG+AGG	+	chr5.2:37240062-37240081	MS.gene064006:CDS	40.0%
	CAGCCTGAAAAGTTATAGCA+GGG	-	chr5.2:37240145-37240164	None:intergenic	40.0%
	GAAATACAATGCCGAGAATC+AGG	+	chr5.2:37239925-37239944	MS.gene064006:CDS	40.0%
	GACATTCTTAAGTCAACCCA+TGG	+	chr5.2:37240059-37240078	MS.gene064006:CDS	40.0%
	TCAGCCTGAAAAGTTATAGC+AGG	-	chr5.2:37240146-37240165	None:intergenic	40.0%
	TGTGTTCGCACTTTGTGTAT+AGG	+	chr5.2:37239608-37239627	MS.gene064006:CDS	40.0%
!!	TCTTGATTTTGATCCTGGAC+AGG	+	chr5.2:37239787-37239806	MS.gene064006:intron	40.0%
	ATAAACGCGCAATTGCTGGA+GGG	-	chr5.2:37239829-37239848	None:intergenic	45.0%
	ATGGCACAAGACTCAACTAC+AGG	-	chr5.2:37240194-37240213	None:intergenic	45.0%
	GATGGAGCCAAAATCGAAAC+CGG	+	chr5.2:37239498-37239517	MS.gene064006:CDS	45.0%
	GGCAGCATCATTTGCTTGAA+AGG	-	chr5.2:37240108-37240127	None:intergenic	45.0%
	TACAATGCCGAGAATCAGGT+AGG	+	chr5.2:37239929-37239948	MS.gene064006:intron	45.0%
	TGAAGAAATTCGAGTCGCCA+TGG	+	chr5.2:37239654-37239673	MS.gene064006:CDS	45.0%
!	AGGCTGAAGTCTTTTGCAGT+AGG	+	chr5.2:37240159-37240178	MS.gene064006:CDS	45.0%
!	CTGCCCTGCTATAACTTTTC+AGG	+	chr5.2:37240139-37240158	MS.gene064006:CDS	45.0%
!!	TCATTGCGTATCGATTCCCA+CGG	-	chr5.2:37239529-37239548	None:intergenic	45.0%
!!!	TTCGATTTTGGCTCCATCGT+CGG	-	chr5.2:37239496-37239515	None:intergenic	45.0%
	AGATCATCGTCCATGCGATC+AGG	-	chr5.2:37239866-37239885	None:intergenic	50.0%
	CATAAACGCGCAATTGCTGG+AGG	-	chr5.2:37239830-37239849	None:intergenic	50.0%
	CCAGCAATTGCGCGTTTATG+CGG	+	chr5.2:37239830-37239849	MS.gene064006:CDS	50.0%
	CTCCAAAGCAAGCTTGCAGA+GGG	-	chr5.2:37239906-37239925	None:intergenic	50.0%
	GCACAAGACTCAACTACAGG+CGG	-	chr5.2:37240191-37240210	None:intergenic	50.0%
	GTTATAGCAGGGCAGTTGGA+AGG	-	chr5.2:37240134-37240153	None:intergenic	50.0%
	TAAACGCGCAATTGCTGGAG+GGG	-	chr5.2:37239828-37239847	None:intergenic	50.0%
	TCCAAAGCAAGCTTGCAGAG+GGG	-	chr5.2:37239905-37239924	None:intergenic	50.0%
	TCTCCAAAGCAAGCTTGCAG+AGG	-	chr5.2:37239907-37239926	None:intergenic	50.0%
	AGAGGGGAGTGAGTTCAAGG+CGG	-	chr5.2:37239889-37239908	None:intergenic	55.0%
	AGCAGGGCAGTTGGAAGGAT+GGG	-	chr5.2:37240129-37240148	None:intergenic	55.0%
	AGCTACCTTACCACCGACGA+TGG	+	chr5.2:37239480-37239499	MS.gene064006:CDS	55.0%
	CCGCATAAACGCGCAATTGC+TGG	-	chr5.2:37239833-37239852	None:intergenic	55.0%
	CGGTATCAGACCTGATCGCA+TGG	+	chr5.2:37239853-37239872	MS.gene064006:CDS	55.0%
	GCAATTGCGCGTTTATGCGG+CGG	+	chr5.2:37239833-37239852	MS.gene064006:CDS	55.0%
	GCGCTTTCTATGCTCAGCCA+TGG	-	chr5.2:37239674-37239693	None:intergenic	55.0%
	TAGCAGGGCAGTTGGAAGGA+TGG	-	chr5.2:37240130-37240149	None:intergenic	55.0%
	TGCAGAGGGGAGTGAGTTCA+AGG	-	chr5.2:37239892-37239911	None:intergenic	55.0%
!	GGCGTGTCCGGTTTCGATTT+TGG	-	chr5.2:37239508-37239527	None:intergenic	55.0%
!	TCCCCTCTGCAAGCTTGCTT+TGG	+	chr5.2:37239901-37239920	MS.gene064006:CDS	55.0%
!!!	GATTTTGGCTCCATCGTCGG+TGG	-	chr5.2:37239493-37239512	None:intergenic	55.0%
	AATCGAAACCGGACACGCCG+TGG	+	chr5.2:37239509-37239528	MS.gene064006:CDS	60.0%
	ATCGAAACCGGACACGCCGT+GGG	+	chr5.2:37239510-37239529	MS.gene064006:CDS	60.0%
	TGGCTCCATCGTCGGTGGTA+AGG	-	chr5.2:37239488-37239507	None:intergenic	60.0%
!	ATCGATTCCCACGGCGTGTC+CGG	-	chr5.2:37239520-37239539	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	37239475	37240226	37239475	ID=MS.gene064006
chr5.2	mRNA	37239475	37240226	37239475	ID=MS.gene064006.t1;Parent=MS.gene064006
chr5.2	exon	37239475	37239708	37239475	ID=MS.gene064006.t1.exon1;Parent=MS.gene064006.t1
chr5.2	CDS	37239475	37239708	37239475	ID=cds.MS.gene064006.t1;Parent=MS.gene064006.t1
chr5.2	exon	37239809	37239946	37239809	ID=MS.gene064006.t1.exon2;Parent=MS.gene064006.t1
chr5.2	CDS	37239809	37239946	37239809	ID=cds.MS.gene064006.t1;Parent=MS.gene064006.t1
chr5.2	exon	37240038	37240226	37240038	ID=MS.gene064006.t1.exon3;Parent=MS.gene064006.t1
chr5.2	CDS	37240038	37240226	37240038	ID=cds.MS.gene064006.t1;Parent=MS.gene064006.t1

Gene Sequence

>MS.gene064006

ATGGAAGCTACCTTACCACCGACGATGGAGCCAAAATCGAAACCGGACACGCCGTGGGAATCGATACGCAATGAAGAACTTATGGGAAGTGACTTTTCTATCTACGACGATCTCGATTATCCTTATATGTTTATGTGTTCGCACTTTGTGTATAGGAATAAGGTTCAGATAAAAAAAGATGAAGAAATTCGAGTCGCCATGGCTGAGCATAGAAAGCGCTCCCGTAACCTAATTGTAAGCATCTATTTATCTATCTCATTTTGCTTTCCAATTTTGCGTTTCACTTAATTTGTTATTAAAATAATCATAATCTCTTGATTTTGATCCTGGACAGGTATTTGATGCTATTTCCCCTCCAGCAATTGCGCGTTTATGCGGCGGTATCAGACCTGATCGCATGGACGATGATCTCCGCCTTGAACTCACTCCCCTCTGCAAGCTTGCTTTGGAGAAATACAATGCCGAGAATCAGGTAGGTTCAATACTTTTTAGAGTAAACTTATGAATATAGTTTATTTATTCATTCATTGATCAACTAATATAGTATTCTTGTTAATTATTAGGGTGCAAATTTTGTGTTTGATGACATTCTTAAGTCAACCCATGGAGGTAATATTGTGTATTACATGACCTTTCAAGCAAATGATGCTGCCCATCCTTCCAACTGCCCTGCTATAACTTTTCAGGCTGAAGTCTTTTGCAGTAGGTCTGAACCGCCTGTAGTTGAGTCTTGTGCCATGAAGCCTACCTAG

Protein sequence

>MS.gene064006.t1

MEATLPPTMEPKSKPDTPWESIRNEELMGSDFSIYDDLDYPYMFMCSHFVYRNKVQIKKDEEIRVAMAEHRKRSRNLIVFDAISPPAIARLCGGIRPDRMDDDLRLELTPLCKLALEKYNAENQGANFVFDDILKSTHGGNIVYYMTFQANDAAHPSNCPAITFQAEVFCSRSEPPVVESCAMKPT