AlfalfaGEDB | MS.gene065867

M. sativa cultivar XinJiangDaYe / MS.gene065867

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12854.10 PF13041.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065867.t1	KEH40695.1	30.2	325	172	7	28	301	37	357	7.40E-22	114.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065867.t1	A0A072VRD3	30.2	325	172	7	28	301	37	357	5.4e-22	114.8

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene065867	0.842392	2.66E-58	-1.69E-46
MS.gene049501	MS.gene065867	0.818532	1.87E-52	-1.69E-46
MS.gene052867	MS.gene065867	0.80467	1.93E-49	-1.69E-46
MS.gene05288	MS.gene065867	0.844919	5.61E-59	-1.69E-46
MS.gene05289	MS.gene065867	0.846965	1.56E-59	-1.69E-46
MS.gene053008	MS.gene065867	0.815227	1.03E-51	-1.69E-46
MS.gene053166	MS.gene065867	0.824551	7.59E-54	-1.69E-46
MS.gene054236	MS.gene065867	0.801891	7.27E-49	-1.69E-46
MS.gene054801	MS.gene065867	0.81367	2.28E-51	-1.69E-46
MS.gene055356	MS.gene065867	0.806958	6.39E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065867.t1	MTR_1g033450	29.538	325	174	7	28	301	37	357	9.48e-29	115
MS.gene065867.t1	MTR_0572s0010	27.442	215	139	2	88	302	224	421	3.19e-21	94.4
MS.gene065867.t1	MTR_1g050495	25.446	224	159	3	88	310	220	436	7.34e-20	90.5
MS.gene065867.t1	MTR_6g079380	25.733	307	175	7	20	310	166	435	9.18e-20	90.1
MS.gene065867.t1	MTR_6g078190	24.828	290	175	5	40	302	64	337	4.20e-19	88.2
MS.gene065867.t1	MTR_3g448620	27.962	211	145	3	88	298	215	418	5.65e-19	87.8
MS.gene065867.t1	MTR_6g079920	25.267	281	196	5	40	312	192	466	1.16e-18	86.7
MS.gene065867.t1	MTR_6g079920	20.922	282	194	5	35	301	292	559	8.97e-12	66.2
MS.gene065867.t1	MTR_6g077540	24.510	306	191	6	20	310	182	462	3.02e-18	85.5
MS.gene065867.t1	MTR_6g077540	20.070	284	194	6	35	301	291	558	8.42e-12	66.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065867.t1	AT5G41170	24.627	268	166	7	65	304	61	320	2.60e-16	79.3
MS.gene065867.t1	AT3G06920	26.728	217	144	4	91	304	612	816	3.20e-16	79.7
MS.gene065867.t1	AT3G06920	26.728	217	144	4	91	304	626	830	3.66e-16	79.7
MS.gene065867.t1	AT3G06920	26.728	217	144	4	91	304	626	830	3.66e-16	79.7
MS.gene065867.t1	AT5G41170	24.627	268	166	7	65	304	161	420	5.38e-16	79.0
MS.gene065867.t1	AT5G41170	24.627	268	166	7	65	304	161	420	5.78e-16	78.6
MS.gene065867.t1	AT5G59900	23.052	308	184	7	37	299	191	490	4.48e-15	76.3
MS.gene065867.t1	AT5G59900	25.094	267	178	8	41	295	370	626	3.73e-11	64.3
MS.gene065867.t1	AT4G28010	23.985	271	176	6	41	295	110	366	1.15e-14	75.1
MS.gene065867.t1	AT1G13040	22.819	298	172	7	41	291	223	509	1.36e-13	71.6

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Find 68 sgRNAs with CRISPR-Local

Find 108 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATAGAAAAGCATCCA+CGG	0.628919	2.4:+31387600	None:intergenic
AACAGCCTTATGGAGGTCTT+TGG	0.388961	2.4:-31387648	MS.gene065867:CDS
AACAGTATAACACGGAAGGT+CGG	0.624195	2.4:+31387347	None:intergenic
AACTCCTTCCTCTTCACAAA+AGG	0.402673	2.4:+31388438	None:intergenic
AAGCTTTAAAAGAAACCGAA+GGG	0.631235	2.4:+31387827	None:intergenic
AAGTGTTGGAAGGATTGAAG+AGG	0.617326	2.4:-31387730	MS.gene065867:CDS
ACAAAGAGTAGTGCTTTCTC+AGG	0.492053	2.4:+31387399	None:intergenic
ACAGAAATGAAGGAGTTCAA+TGG	0.498224	2.4:-31388471	MS.gene065867:CDS
ACTCCTTCCTCTTCACAAAA+GGG	0.531722	2.4:+31388439	None:intergenic
ACTGTCAATACCCTATTGAA+GGG	0.569698	2.4:-31388249	MS.gene065867:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTAGTATACATTACCACAT+CGG	+	chr2.4:31387841-31387860	None:intergenic	30.0%
ATCCACTCATCAATAATTCA+TGG	-	chr2.4:31387954-31387973	MS.gene065867:intron	30.0%
ATCTTCTTCATCATCTTCTA+TGG	-	chr2.4:31387298-31387317	MS.gene065867:CDS	30.0%
ATGAATGCAAAACCAAAACA+AGG	-	chr2.4:31387614-31387633	MS.gene065867:CDS	30.0%
CGTCTTTGCAACTTAAATTT+AGG	-	chr2.4:31387497-31387516	MS.gene065867:CDS	30.0%
CTCATGAAAACAGTATAACA+CGG	+	chr2.4:31388497-31388516	None:intergenic	30.0%
TCTGTTAATTACTGCAGTAA+AGG	+	chr2.4:31388291-31388310	None:intergenic	30.0%
TCTTCTTCATCATCTTCTAT+GGG	-	chr2.4:31387299-31387318	MS.gene065867:CDS	30.0%
TGACTTGATGTCAAAATATC+TGG	+	chr2.4:31388039-31388058	None:intergenic	30.0%
TGATAATATCTTAGGTGTCA+AGG	-	chr2.4:31387815-31387834	MS.gene065867:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	31387257	31388598	31387257	ID=MS.gene065867
chr2.4	mRNA	31387257	31388598	31387257	ID=MS.gene065867.t1;Parent=MS.gene065867
chr2.4	exon	31388418	31388598	31388418	ID=MS.gene065867.t1.exon1;Parent=MS.gene065867.t1
chr2.4	CDS	31388418	31388598	31388418	ID=cds.MS.gene065867.t1;Parent=MS.gene065867.t1
chr2.4	exon	31388249	31388336	31388249	ID=MS.gene065867.t1.exon2;Parent=MS.gene065867.t1
chr2.4	CDS	31388249	31388336	31388249	ID=cds.MS.gene065867.t1;Parent=MS.gene065867.t1
chr2.4	exon	31387257	31387938	31387257	ID=MS.gene065867.t1.exon3;Parent=MS.gene065867.t1
chr2.4	CDS	31387257	31387938	31387257	ID=cds.MS.gene065867.t1;Parent=MS.gene065867.t1

Gene Sequence

>MS.gene065867

ATGAATTACAAATTTTGTTCGTCATTTTACAGGGTTTACTCATCTTCTTCATCATCTTCTATGGGATTTGAGATTCAAACAATTCGTGCAATGATGAAGAAACGAACAGAAATGAAGGAGTTCAATGGGTTACTACACCCTTTTGTGAAGAGGAAGGAGTTCCGCAGCGCTCTCTATCTGGGTAGGTATCTTTTTATCAACAAAAGATACGATTCTCATACTTTGAATGTCTATATTAACCGTCTTTGCAACTTAAATTTAGGGTTTCGTTATGCAATGTCTGTTGTTGGCTTGTTTGTTAAATCTGGGTTAGAGATTGACATTGTTACTGTCAATACCCTATTGAAGGGGTATGCCATGAATGCAAAACCAAAACAAGGTTTAGAGTTTGCTGCCAAGTTACAGGGTGTAGGGTTCCAGTTAAATGAAATTTCCTTTGGGATAATCCTTCAATCCATATGCATGTTAGGTGATCTTAGTGGAGTTTTGAAATTATTGCGCAGTATGCATGTTTGGTGATCTTAGTGGAGTTTTGAAATTATTGCGCAGTATTGTAAATGATAATATCTTAGGTGTCAAGGCCGATGTGGTAATGTATACTACTTTTATAGATGCATTGTGCAAACACGGCTGTGTTAATGATGCTATTGCCACTTACAGTGATATGGTTAACGCGCATATTGAACCCACTTATTTCATCCACTCATCAATAATTCATGGCCACTGCCTTGTTAAGCAACCGGAACAAGCCCTTCGGTTTCTTTTAAAGCTTGTCTACACCAGATATTTTGACATCAAGTCATACTTAGATATTACCTCATTTGGCTCTCTGATGGACTATTTTGGAAGTGTTGGAAGGATTGAAGAGGCTTTGCTCTTACTTTCTATCATGATAAAATTGGGAATACAGCCCAATCTGGTTGTACTCAACAGCCTTATGGAGGTCTTTGGTCATGCGTGTGAAAATGGTCGAAATCCGTGGATGCTTTTCTATGTTTTAGGGAGACTTAGATTCACCCCAAATGCGAAGTCCTTTACTGCAGTAATTAACAGATTTCTTAGATTTGAAAAGGTTGATGATGCTTTGAAAATTTTAGATCACATGAAGTCTAAATACATCAACGTTGATCAGAAATTGTTAACGTCTGTGGTAAATGGCCTAATAAGCAATGCTCAACCTGAGAAAGCACTACTCTTTGTTGACATGTTCATAGAGAAAAAGTTTAAGGCCGACCTTCCGTGTTATACTGTTTTCATGAGGGCTTGTTTTGAAGGCAACGAACACAAACGTGCCCTTGATGGTGGAACCAAAGGAAAAGAGGGTTTTGTTACTTCACAATAA

Protein sequence

>MS.gene065867.t1

MNYKFCSSFYRVYSSSSSSSMGFEIQTIRAMMKKRTEMKEFNGLLHPFVKRKEFRSALYLGFRYAMSVVGLFVKSGLEIDIVTVNTLLKGDMVNAHIEPTYFIHSSIIHGHCLVKQPEQALRFLLKLVYTRYFDIKSYLDITSFGSLMDYFGSVGRIEEALLLLSIMIKLGIQPNLVVLNSLMEVFGHACENGRNPWMLFYVLGRLRFTPNAKSFTAVINRFLRFEKVDDALKILDHMKSKYINVDQKLLTSVVNGLISNAQPEKALLFVDMFIEKKFKADLPCYTVFMRACFEGNEHKRALDGGTKGKEGFVTSQ