Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066988.t1 | Q03460.1 | 94.4 | 2194 | 19 | 9 | 1 | 2091 | 1 | 2194 | 0.00E+00 | 4084.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066988.t1 | Q03460 | 94.4 | 2194 | 19 | 9 | 1 | 2091 | 1 | 2194 | 0.0e+00 | 4084.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066988.t1 | A0A072VE66 | 94.1 | 2194 | 27 | 9 | 1 | 2091 | 1 | 2194 | 0.0e+00 | 4065.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene066988 | -0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene049627 | MS.gene066988 | -0.816185 | 6.30E-52 | -1.69E-46 |
| MS.gene050170 | MS.gene066988 | -0.830282 | 3.21E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene066988 | -0.802496 | 5.46E-49 | -1.69E-46 |
| MS.gene050747 | MS.gene066988 | -0.803293 | 3.74E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene066988 | -0.81653 | 5.27E-52 | -1.69E-46 |
| MS.gene052015 | MS.gene066988 | 0.817444 | 3.29E-52 | -1.69E-46 |
| MS.gene053203 | MS.gene066988 | -0.816838 | 4.50E-52 | -1.69E-46 |
| MS.gene053421 | MS.gene066988 | 0.820994 | 5.11E-53 | -1.69E-46 |
| MS.gene05470 | MS.gene066988 | -0.802442 | 5.60E-49 | -1.69E-46 |
| MS.gene055577 | MS.gene066988 | -0.816283 | 5.99E-52 | -1.69E-46 |
| MS.gene056610 | MS.gene066988 | -0.816605 | 5.07E-52 | -1.69E-46 |
| MS.gene057831 | MS.gene066988 | -0.803323 | 3.68E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene066988 | -0.804938 | 1.70E-49 | -1.69E-46 |
| MS.gene05837 | MS.gene066988 | -0.822158 | 2.75E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene066988 | -0.825349 | 4.92E-54 | -1.69E-46 |
| MS.gene059303 | MS.gene066988 | -0.806272 | 8.92E-50 | -1.69E-46 |
| MS.gene059801 | MS.gene066988 | -0.812187 | 4.81E-51 | -1.69E-46 |
| MS.gene059837 | MS.gene066988 | -0.803107 | 4.08E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene066988 | -0.80068 | 1.29E-48 | -1.69E-46 |
| MS.gene060595 | MS.gene066988 | 0.802188 | 6.32E-49 | -1.69E-46 |
| MS.gene060602 | MS.gene066988 | 0.800147 | 1.65E-48 | -1.69E-46 |
| MS.gene06077 | MS.gene066988 | -0.83532 | 1.80E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene066988 | MS.gene51146 | PPI |
| MS.gene024049 | MS.gene066988 | PPI |
| MS.gene066988 | MS.gene033486 | PPI |
| MS.gene066988 | MS.gene52590 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066988.t1 | MTR_1g027020 | 94.075 | 2194 | 27 | 9 | 1 | 2091 | 1 | 2194 | 0.0 | 4201 |
| MS.gene066988.t1 | MTR_7g089970 | 42.014 | 1559 | 755 | 30 | 88 | 1551 | 82 | 1586 | 0.0 | 1136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066988.t1 | AT5G53460 | 77.868 | 2205 | 362 | 16 | 2 | 2091 | 14 | 2207 | 0.0 | 3486 |
| MS.gene066988.t1 | AT5G53460 | 77.868 | 2205 | 362 | 16 | 2 | 2091 | 14 | 2207 | 0.0 | 3486 |
| MS.gene066988.t1 | AT5G53460 | 77.868 | 2205 | 362 | 16 | 2 | 2091 | 14 | 2207 | 0.0 | 3486 |
| MS.gene066988.t1 | AT5G04140 | 43.177 | 1561 | 750 | 30 | 80 | 1551 | 84 | 1596 | 0.0 | 1164 |
| MS.gene066988.t1 | AT2G41220 | 42.447 | 1569 | 749 | 31 | 80 | 1551 | 86 | 1597 | 0.0 | 1161 |
| MS.gene066988.t1 | AT5G04140 | 43.254 | 1549 | 742 | 30 | 92 | 1551 | 122 | 1622 | 0.0 | 1160 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10239 | gene | 2386 | 13907 | 2386 | ID=MS.gene066988 |
| 10239 | mRNA | 2386 | 13907 | 2386 | ID=MS.gene066988.t1;Parent=MS.gene066988 |
| 10239 | exon | 13554 | 13907 | 13554 | ID=MS.gene066988.t1.exon1;Parent=MS.gene066988.t1 |
| 10239 | CDS | 13554 | 13907 | 13554 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 13138 | 13257 | 13138 | ID=MS.gene066988.t1.exon2;Parent=MS.gene066988.t1 |
| 10239 | CDS | 13138 | 13257 | 13138 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 12038 | 12142 | 12038 | ID=MS.gene066988.t1.exon3;Parent=MS.gene066988.t1 |
| 10239 | CDS | 12038 | 12142 | 12038 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 11523 | 11675 | 11523 | ID=MS.gene066988.t1.exon4;Parent=MS.gene066988.t1 |
| 10239 | CDS | 11523 | 11675 | 11523 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 11211 | 11311 | 11211 | ID=MS.gene066988.t1.exon5;Parent=MS.gene066988.t1 |
| 10239 | CDS | 11211 | 11311 | 11211 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 11004 | 11100 | 11004 | ID=MS.gene066988.t1.exon6;Parent=MS.gene066988.t1 |
| 10239 | CDS | 11004 | 11100 | 11004 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 10422 | 10531 | 10422 | ID=MS.gene066988.t1.exon7;Parent=MS.gene066988.t1 |
| 10239 | CDS | 10422 | 10531 | 10422 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 9984 | 10086 | 9984 | ID=MS.gene066988.t1.exon8;Parent=MS.gene066988.t1 |
| 10239 | CDS | 9984 | 10086 | 9984 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 9721 | 9877 | 9721 | ID=MS.gene066988.t1.exon9;Parent=MS.gene066988.t1 |
| 10239 | CDS | 9721 | 9877 | 9721 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 9289 | 9632 | 9289 | ID=MS.gene066988.t1.exon10;Parent=MS.gene066988.t1 |
| 10239 | CDS | 9289 | 9632 | 9289 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 9136 | 9206 | 9136 | ID=MS.gene066988.t1.exon11;Parent=MS.gene066988.t1 |
| 10239 | CDS | 9136 | 9206 | 9136 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 8673 | 9043 | 8673 | ID=MS.gene066988.t1.exon12;Parent=MS.gene066988.t1 |
| 10239 | CDS | 8673 | 9043 | 8673 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 8573 | 8641 | 8573 | ID=MS.gene066988.t1.exon13;Parent=MS.gene066988.t1 |
| 10239 | CDS | 8573 | 8641 | 8573 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 7758 | 8472 | 7758 | ID=MS.gene066988.t1.exon14;Parent=MS.gene066988.t1 |
| 10239 | CDS | 7758 | 8472 | 7758 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 7616 | 7728 | 7616 | ID=MS.gene066988.t1.exon15;Parent=MS.gene066988.t1 |
| 10239 | CDS | 7616 | 7728 | 7616 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 7236 | 7390 | 7236 | ID=MS.gene066988.t1.exon16;Parent=MS.gene066988.t1 |
| 10239 | CDS | 7236 | 7390 | 7236 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 6790 | 6951 | 6790 | ID=MS.gene066988.t1.exon17;Parent=MS.gene066988.t1 |
| 10239 | CDS | 6790 | 6951 | 6790 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 6045 | 6692 | 6045 | ID=MS.gene066988.t1.exon18;Parent=MS.gene066988.t1 |
| 10239 | CDS | 6045 | 6692 | 6045 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 5646 | 6000 | 5646 | ID=MS.gene066988.t1.exon19;Parent=MS.gene066988.t1 |
| 10239 | CDS | 5646 | 6000 | 5646 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 5255 | 5616 | 5255 | ID=MS.gene066988.t1.exon20;Parent=MS.gene066988.t1 |
| 10239 | CDS | 5255 | 5616 | 5255 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 5091 | 5204 | 5091 | ID=MS.gene066988.t1.exon21;Parent=MS.gene066988.t1 |
| 10239 | CDS | 5091 | 5204 | 5091 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 4670 | 4741 | 4670 | ID=MS.gene066988.t1.exon22;Parent=MS.gene066988.t1 |
| 10239 | CDS | 4670 | 4741 | 4670 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 4542 | 4625 | 4542 | ID=MS.gene066988.t1.exon23;Parent=MS.gene066988.t1 |
| 10239 | CDS | 4542 | 4625 | 4542 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 4175 | 4443 | 4175 | ID=MS.gene066988.t1.exon24;Parent=MS.gene066988.t1 |
| 10239 | CDS | 4175 | 4443 | 4175 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 3697 | 4002 | 3697 | ID=MS.gene066988.t1.exon25;Parent=MS.gene066988.t1 |
| 10239 | CDS | 3697 | 4002 | 3697 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 3469 | 3590 | 3469 | ID=MS.gene066988.t1.exon26;Parent=MS.gene066988.t1 |
| 10239 | CDS | 3469 | 3590 | 3469 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 3322 | 3425 | 3322 | ID=MS.gene066988.t1.exon27;Parent=MS.gene066988.t1 |
| 10239 | CDS | 3322 | 3425 | 3322 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 2976 | 3233 | 2976 | ID=MS.gene066988.t1.exon28;Parent=MS.gene066988.t1 |
| 10239 | CDS | 2976 | 3233 | 2976 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| 10239 | exon | 2386 | 2667 | 2386 | ID=MS.gene066988.t1.exon29;Parent=MS.gene066988.t1 |
| 10239 | CDS | 2386 | 2667 | 2386 | ID=cds.MS.gene066988.t1;Parent=MS.gene066988.t1 |
| Gene Sequence |
| Protein sequence |
