AlfalfaGEDB | MS.gene068726

M. sativa cultivar XinJiangDaYe / MS.gene068726

KEGG: -
GO: -
PFAM: PF04935.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068726.t1	KEH38713.1	75.5	143	33	1	49	191	85	225	9.60E-41	176.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068726.t1	A0A072VB89	75.5	143	33	1	49	191	85	225	6.9e-41	176.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049877	MS.gene068726	0.801996	6.92E-49	-1.69E-46
MS.gene050227	MS.gene068726	0.800719	1.26E-48	-1.69E-46
MS.gene050228	MS.gene068726	0.80305	4.19E-49	-1.69E-46
MS.gene050237	MS.gene068726	0.845331	4.34E-59	-1.69E-46
MS.gene050442	MS.gene068726	0.809548	1.79E-50	-1.69E-46
MS.gene050799	MS.gene068726	0.81207	5.10E-51	-1.69E-46
MS.gene050824	MS.gene068726	0.80214	6.46E-49	-1.69E-46
MS.gene052190	MS.gene068726	0.819124	1.37E-52	-1.69E-46
MS.gene052358	MS.gene068726	-0.813855	2.07E-51	-1.69E-46
MS.gene052519	MS.gene068726	0.811299	7.51E-51	-1.69E-46
MS.gene052863	MS.gene068726	0.811627	6.37E-51	-1.69E-46
MS.gene053006	MS.gene068726	0.815452	9.18E-52	-1.69E-46
MS.gene053221	MS.gene068726	0.801232	9.92E-49	-1.69E-46
MS.gene05415	MS.gene068726	0.808818	2.57E-50	-1.69E-46
MS.gene054291	MS.gene068726	-0.803894	2.80E-49	-1.69E-46
MS.gene054472	MS.gene068726	0.813279	2.77E-51	-1.69E-46
MS.gene055818	MS.gene068726	0.847157	1.38E-59	-1.69E-46
MS.gene056506	MS.gene068726	0.809857	1.54E-50	-1.69E-46
MS.gene057026	MS.gene068726	0.811521	6.72E-51	-1.69E-46
MS.gene058037	MS.gene068726	0.816654	4.95E-52	-1.69E-46
MS.gene058625	MS.gene068726	0.818662	1.74E-52	-1.69E-46
MS.gene058636	MS.gene068726	0.804064	2.58E-49	-1.69E-46
MS.gene058979	MS.gene068726	0.809353	1.97E-50	-1.69E-46
MS.gene05954	MS.gene068726	0.801842	7.44E-49	-1.69E-46
MS.gene059789	MS.gene068726	0.888284	6.77E-73	-1.69E-46
MS.gene060322	MS.gene068726	0.838272	3.17E-57	-1.69E-46
MS.gene060572	MS.gene068726	0.818627	1.78E-52	-1.69E-46
MS.gene060634	MS.gene068726	-0.809666	1.69E-50	-1.69E-46
MS.gene060683	MS.gene068726	0.810022	1.42E-50	-1.69E-46
MS.gene060685	MS.gene068726	0.810992	8.75E-51	-1.69E-46
MS.gene061040	MS.gene068726	-0.827983	1.16E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068726.t1	MTR_2g079000	79.021	143	28	1	49	191	85	225	2.07e-68	208
MS.gene068726.t1	MTR_5g012820	61.929	197	48	4	10	179	109	305	3.66e-65	204
MS.gene068726.t1	MTR_5g012820	61.929	197	48	4	10	179	119	315	7.16e-65	204
MS.gene068726.t1	MTR_2g079040	87.500	40	5	0	133	172	1	40	7.74e-16	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068726.t1	AT5G05210	45.588	204	79	6	1	179	144	340	9.44e-41	142
MS.gene068726.t1	AT5G05210	45.588	204	79	6	1	179	136	332	1.09e-40	142
MS.gene068726.t1	AT2G27750	45.946	111	54	3	74	179	39	148	4.66e-20	83.2

Find 46 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTGAAGCTATTGCTAAAA+AGG	0.287342	2.3:+18915327	MS.gene068726:CDS
ATACTTCTCTTTATCAGTTT+AGG	0.301257	2.3:-18915403	None:intergenic
TGAAAGAAAGATAGAGTTTC+CGG	0.308185	2.3:+18915141	MS.gene068726:CDS
AGGTCCTAACTGTGCAAATC+TGG	0.341251	2.3:+18915099	MS.gene068726:CDS
ATCGCAAACTTGAAGAGTTT+CGG	0.345780	2.3:+18915074	MS.gene068726:CDS
GCGGTTAAGCCTTTCATGTC+GGG	0.372922	2.3:+18914998	MS.gene068726:CDS
AGCAGAAAATCGCAGTGAAA+TGG	0.374864	2.3:+18915446	MS.gene068726:CDS
TGAAAGAGCTAAGAAGTTAG+AGG	0.400614	2.3:+18915279	MS.gene068726:CDS
GTATACAGAAATGGAAGAAT+AGG	0.408150	2.3:+18915422	MS.gene068726:CDS
GCAATAGCTTCACCCTTCTC+AGG	0.419537	2.3:-18915319	None:intergenic
AGCAGCAATGAATAGAGCTT+CGG	0.425070	2.3:+18915360	MS.gene068726:CDS
GCAGCAATGAATAGAGCTTC+GGG	0.426903	2.3:+18915361	MS.gene068726:CDS
TCTTTAAAAGAGCTTGTGTT+TGG	0.437000	2.3:+18915193	MS.gene068726:CDS
TCCTTTACCACCTTCTCCTC+CGG	0.440386	2.3:-18915160	None:intergenic
ATCTGGAAAATGCTAATAAG+AGG	0.444036	2.3:+18915116	MS.gene068726:CDS
TAAAGAGAAGTATACAGAAA+TGG	0.461758	2.3:+18915413	MS.gene068726:CDS
GCAGAAAATCGCAGTGAAAT+GGG	0.472714	2.3:+18915447	MS.gene068726:CDS
TCTTTCAAGTTCCTTGTGCT+TGG	0.476271	2.3:-18915263	None:intergenic
GGCAATGTTAAACTTCAGAA+TGG	0.487362	2.3:+18915214	MS.gene068726:CDS
GATAGAGTTTCCGGAGGAGA+AGG	0.496889	2.3:+18915150	MS.gene068726:CDS
GAATAGAGCTTCGGGAATCA+AGG	0.497557	2.3:+18915369	MS.gene068726:CDS
TGCGGTTAAGCCTTTCATGT+CGG	0.509135	2.3:+18914997	MS.gene068726:CDS
TCCGGAGGAGAAGGTGGTAA+AGG	0.513098	2.3:+18915159	MS.gene068726:CDS
AAACTTGAAGAGTTTCGGTC+AGG	0.519958	2.3:+18915079	MS.gene068726:CDS
TAAGCCTTTCATGTCGGGGT+TGG	0.521349	2.3:+18915003	MS.gene068726:CDS
TCAAACAAAGGATCAGTTGA+AGG	0.525525	2.3:+18915487	MS.gene068726:CDS
CGCAGTGAAATGGGAAGAGA+GGG	0.547718	2.3:+18915456	MS.gene068726:CDS
CCTTTCGTGTAGTTCTTCAA+AGG	0.548079	2.3:-18915047	None:intergenic
GCTTCCAACCCCGACATGAA+AGG	0.556004	2.3:-18915007	None:intergenic
CGGGGTTGGAAGCAGATGAT+CGG	0.561569	2.3:+18915017	MS.gene068726:CDS
CTTCAGAATGGTGAAATGCA+AGG	0.562609	2.3:+18915226	MS.gene068726:CDS
TTCAGAATGGTGAAATGCAA+GGG	0.568164	2.3:+18915227	MS.gene068726:CDS
AGAGTTTCCGGAGGAGAAGG+TGG	0.570130	2.3:+18915153	MS.gene068726:CDS
AATGACAGCGATGGAGAGGA+AGG	0.580649	2.3:+18914974	MS.gene068726:CDS
CGGTTAAGCCTTTCATGTCG+GGG	0.581496	2.3:+18914999	MS.gene068726:CDS
AATGAATGACAGCGATGGAG+AGG	0.585376	2.3:+18914970	None:intergenic
GAAGAAGAATGATCCTGAGA+AGG	0.589096	2.3:+18915306	MS.gene068726:CDS
AAGAAGAATGATCCTGAGAA+GGG	0.591026	2.3:+18915307	MS.gene068726:CDS
TCTGGAAAATGCTAATAAGA+GGG	0.594774	2.3:+18915117	MS.gene068726:CDS
TCGCAGTGAAATGGGAAGAG+AGG	0.597560	2.3:+18915455	MS.gene068726:CDS
AGGGTTCAAAGTTCAAACAA+AGG	0.644899	2.3:+18915475	MS.gene068726:CDS
GAGAAAAGTTTCCAAGCACA+AGG	0.645702	2.3:+18915252	MS.gene068726:CDS
GGAGGAGAAGGTGGTAAAGG+AGG	0.646856	2.3:+18915162	MS.gene068726:CDS
AAGAAAGATAGAGTTTCCGG+AGG	0.655190	2.3:+18915144	MS.gene068726:CDS
CCTTTGAAGAACTACACGAA+AGG	0.661334	2.3:+18915047	MS.gene068726:CDS
CAGCGATGGAGAGGAAGGTG+CGG	0.700646	2.3:+18914979	MS.gene068726:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATACTTCTCTTTATCAGTTT+AGG	-	chr2.3:18915406-18915425	None:intergenic	25.0%
!!	TAAAGAGAAGTATACAGAAA+TGG	+	chr2.3:18915413-18915432	MS.gene068726:CDS	25.0%
	ATCTGGAAAATGCTAATAAG+AGG	+	chr2.3:18915116-18915135	MS.gene068726:CDS	30.0%
	GTATACAGAAATGGAAGAAT+AGG	+	chr2.3:18915422-18915441	MS.gene068726:CDS	30.0%
	TCTGGAAAATGCTAATAAGA+GGG	+	chr2.3:18915117-18915136	MS.gene068726:CDS	30.0%
	TGAAAGAAAGATAGAGTTTC+CGG	+	chr2.3:18915141-18915160	MS.gene068726:CDS	30.0%
!	TCTTTAAAAGAGCTTGTGTT+TGG	+	chr2.3:18915193-18915212	MS.gene068726:CDS	30.0%
	AAGAAGAATGATCCTGAGAA+GGG	+	chr2.3:18915307-18915326	MS.gene068726:CDS	35.0%
	AGGCAGAGAAACAACAAAAA+AGG	+	chr2.3:18915507-18915526	MS.gene068726:CDS	35.0%
	AGGGTTCAAAGTTCAAACAA+AGG	+	chr2.3:18915475-18915494	MS.gene068726:CDS	35.0%
	ATCGCAAACTTGAAGAGTTT+CGG	+	chr2.3:18915074-18915093	MS.gene068726:CDS	35.0%
	CTATTGCTAAAAAGGAAGCA+TGG	+	chr2.3:18915335-18915354	MS.gene068726:CDS	35.0%
	GGCAATGTTAAACTTCAGAA+TGG	+	chr2.3:18915214-18915233	MS.gene068726:CDS	35.0%
	TCAAACAAAGGATCAGTTGA+AGG	+	chr2.3:18915487-18915506	MS.gene068726:CDS	35.0%
	TGAAAGAGCTAAGAAGTTAG+AGG	+	chr2.3:18915279-18915298	MS.gene068726:CDS	35.0%
	TTCAGAATGGTGAAATGCAA+GGG	+	chr2.3:18915227-18915246	MS.gene068726:CDS	35.0%
!	TTTTCCAGATTTGCACAGTT+AGG	-	chr2.3:18915106-18915125	None:intergenic	35.0%
!!	AAGCTCTTTTAAAGACTCTG+TGG	-	chr2.3:18915188-18915207	None:intergenic	35.0%
	AAACTTGAAGAGTTTCGGTC+AGG	+	chr2.3:18915079-18915098	MS.gene068726:CDS	40.0%
	AAGAAAGATAGAGTTTCCGG+AGG	+	chr2.3:18915144-18915163	MS.gene068726:CDS	40.0%
	AGCAGAAAATCGCAGTGAAA+TGG	+	chr2.3:18915446-18915465	MS.gene068726:CDS	40.0%
	AGCAGCAATGAATAGAGCTT+CGG	+	chr2.3:18915360-18915379	MS.gene068726:CDS	40.0%
	CCTTTCGTGTAGTTCTTCAA+AGG	-	chr2.3:18915050-18915069	None:intergenic	40.0%
	CTTCAGAATGGTGAAATGCA+AGG	+	chr2.3:18915226-18915245	MS.gene068726:CDS	40.0%
	GAAGAAGAATGATCCTGAGA+AGG	+	chr2.3:18915306-18915325	MS.gene068726:CDS	40.0%
	GAGAAAAGTTTCCAAGCACA+AGG	+	chr2.3:18915252-18915271	MS.gene068726:CDS	40.0%
	GCAGAAAATCGCAGTGAAAT+GGG	+	chr2.3:18915447-18915466	MS.gene068726:CDS	40.0%
	GGGTGAAGCTATTGCTAAAA+AGG	+	chr2.3:18915327-18915346	MS.gene068726:CDS	40.0%
!	CCTTTGAAGAACTACACGAA+AGG	+	chr2.3:18915047-18915066	MS.gene068726:CDS	40.0%
!!	TCTTTCAAGTTCCTTGTGCT+TGG	-	chr2.3:18915266-18915285	None:intergenic	40.0%
	AGGTCCTAACTGTGCAAATC+TGG	+	chr2.3:18915099-18915118	MS.gene068726:CDS	45.0%
	GAATAGAGCTTCGGGAATCA+AGG	+	chr2.3:18915369-18915388	MS.gene068726:CDS	45.0%
	GCAGCAATGAATAGAGCTTC+GGG	+	chr2.3:18915361-18915380	MS.gene068726:CDS	45.0%
	TGCGGTTAAGCCTTTCATGT+CGG	+	chr2.3:18914997-18915016	MS.gene068726:CDS	45.0%
	AATGACAGCGATGGAGAGGA+AGG	+	chr2.3:18914974-18914993	MS.gene068726:CDS	50.0%
	CGCAGTGAAATGGGAAGAGA+GGG	+	chr2.3:18915456-18915475	MS.gene068726:CDS	50.0%
	CGGTTAAGCCTTTCATGTCG+GGG	+	chr2.3:18914999-18915018	MS.gene068726:CDS	50.0%
	GATAGAGTTTCCGGAGGAGA+AGG	+	chr2.3:18915150-18915169	MS.gene068726:CDS	50.0%
	GCAATAGCTTCACCCTTCTC+AGG	-	chr2.3:18915322-18915341	None:intergenic	50.0%
	GCGGTTAAGCCTTTCATGTC+GGG	+	chr2.3:18914998-18915017	MS.gene068726:CDS	50.0%
	TAAGCCTTTCATGTCGGGGT+TGG	+	chr2.3:18915003-18915022	MS.gene068726:CDS	50.0%
	TCCTTTACCACCTTCTCCTC+CGG	-	chr2.3:18915163-18915182	None:intergenic	50.0%
	TCGCAGTGAAATGGGAAGAG+AGG	+	chr2.3:18915455-18915474	MS.gene068726:CDS	50.0%
	AGAGTTTCCGGAGGAGAAGG+TGG	+	chr2.3:18915153-18915172	MS.gene068726:CDS	55.0%
	CGGGGTTGGAAGCAGATGAT+CGG	+	chr2.3:18915017-18915036	MS.gene068726:CDS	55.0%
	GCTTCCAACCCCGACATGAA+AGG	-	chr2.3:18915010-18915029	None:intergenic	55.0%
	GGAGGAGAAGGTGGTAAAGG+AGG	+	chr2.3:18915162-18915181	MS.gene068726:CDS	55.0%
	TCCGGAGGAGAAGGTGGTAA+AGG	+	chr2.3:18915159-18915178	MS.gene068726:CDS	55.0%
	CAGCGATGGAGAGGAAGGTG+CGG	+	chr2.3:18914979-18914998	MS.gene068726:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	18914971	18915546	18914971	ID=MS.gene068726
chr2.3	mRNA	18914971	18915546	18914971	ID=MS.gene068726.t1;Parent=MS.gene068726
chr2.3	exon	18914971	18915546	18914971	ID=MS.gene068726.t1.exon1;Parent=MS.gene068726.t1
chr2.3	CDS	18914971	18915546	18914971	ID=cds.MS.gene068726.t1;Parent=MS.gene068726.t1

Gene Sequence

>MS.gene068726

ATGAATGACAGCGATGGAGAGGAAGGTGCGGTTAAGCCTTTCATGTCGGGGTTGGAAGCAGATGATCGGTTTACGACCTTTGAAGAACTACACGAAAGGCTTCATCGCAAACTTGAAGAGTTTCGGTCAGGTCCTAACTGTGCAAATCTGGAAAATGCTAATAAGAGGGATGAAAGAAAGATAGAGTTTCCGGAGGAGAAGGTGGTAAAGGAGGCCACAGAGTCTTTAAAAGAGCTTGTGTTTGGCAATGTTAAACTTCAGAATGGTGAAATGCAAGGGAAGAGAAAAGTTTCCAAGCACAAGGAACTTGAAAGAGCTAAGAAGTTAGAGGAAGTGAAGAAGAATGATCCTGAGAAGGGTGAAGCTATTGCTAAAAAGGAAGCATGGAAAGCAGCAATGAATAGAGCTTCGGGAATCAAGGTTCATGATGATCCTAAACTGATAAAGAGAAGTATACAGAAATGGAAGAATAGGCAGCAGAAAATCGCAGTGAAATGGGAAGAGAGGGTTCAAAGTTCAAACAAAGGATCAGTTGAAGGCAGAGAAACAACAAAAAAGGTATGCAAATATAGCTGA

Protein sequence

>MS.gene068726.t1

MNDSDGEEGAVKPFMSGLEADDRFTTFEELHERLHRKLEEFRSGPNCANLENANKRDERKIEFPEEKVVKEATESLKELVFGNVKLQNGEMQGKRKVSKHKELERAKKLEEVKKNDPEKGEAIAKKEAWKAAMNRASGIKVHDDPKLIKRSIQKWKNRQQKIAVKWEERVQSSNKGSVEGRETTKKVCKYS