AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07218


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07218.t1 MTR_1g088540 93.333 150 10 0 48 197 2 151 1.37e-99 285
MS.gene07218.t1 MTR_7g108790 68.927 177 53 1 22 196 21 197 2.69e-88 258
MS.gene07218.t1 MTR_4g120825 35.437 206 118 4 1 191 1 206 5.68e-36 125
MS.gene07218.t1 MTR_4g120830 35.437 206 118 4 1 191 1 206 9.23e-36 124
MS.gene07218.t1 MTR_4g035880 35.789 190 105 3 11 189 12 195 6.74e-34 119
MS.gene07218.t1 MTR_8g104765 34.419 215 117 5 1 191 1 215 1.07e-33 119
MS.gene07218.t1 MTR_4g035905 34.715 193 109 4 8 189 9 195 1.02e-32 116
MS.gene07218.t1 MTR_4g035870 34.715 193 109 3 8 189 9 195 1.82e-32 115
MS.gene07218.t1 MTR_2g043560 39.181 171 92 4 27 192 29 192 1.05e-28 106
MS.gene07218.t1 MTR_3g008840 31.875 160 105 2 33 188 26 185 4.94e-28 104
MS.gene07218.t1 MTR_5g012000 30.811 185 120 3 10 189 29 210 6.28e-27 102
MS.gene07218.t1 MTR_3g008820 30.000 160 108 2 33 188 25 184 2.81e-26 99.8
MS.gene07218.t1 MTR_4g124855 30.159 189 120 5 7 186 11 196 5.40e-22 89.4
MS.gene07218.t1 MTR_4g101760 26.000 200 136 4 1 188 1 200 7.96e-21 85.9
MS.gene07218.t1 MTR_4g025610 30.769 169 102 4 33 188 33 199 4.26e-20 84.3
MS.gene07218.t1 MTR_4g025590 30.769 169 102 4 33 188 4 170 9.33e-20 82.8
MS.gene07218.t1 MTR_4g025550 29.592 196 120 6 8 188 7 199 2.18e-19 82.4
MS.gene07218.t1 MTR_4g025620 27.411 197 126 5 5 188 7 199 7.32e-18 78.6
MS.gene07218.t1 MTR_3g008760 26.415 159 110 4 33 185 67 224 1.62e-16 75.1
MS.gene07218.t1 MTR_0036s0140 28.652 178 115 5 8 183 54 221 1.37e-14 70.1
MS.gene07218.t1 MTR_8g023150 29.193 161 103 3 32 186 76 231 3.32e-14 70.5
MS.gene07218.t1 MTR_4g035855 28.750 160 108 3 33 187 44 202 4.24e-14 68.2
MS.gene07218.t1 MTR_4g120290 27.950 161 110 2 28 187 54 209 1.12e-13 68.9
MS.gene07218.t1 MTR_7g112580 26.943 193 124 5 8 186 5 194 1.60e-13 68.6
MS.gene07218.t1 MTR_8g023310 30.818 159 105 3 33 186 68 226 2.78e-13 67.8
MS.gene07218.t1 MTR_1g083470 28.571 161 107 3 33 189 58 214 4.69e-13 67.4
MS.gene07218.t1 MTR_1g083500 28.571 161 107 3 33 189 58 214 4.69e-13 67.4
MS.gene07218.t1 MTR_7g112590 24.204 157 117 2 33 189 59 213 9.05e-12 63.5
MS.gene07218.t1 MTR_4g120280 26.875 160 113 1 28 187 54 209 1.21e-11 63.2
MS.gene07218.t1 MTR_1g008140 27.168 173 113 3 22 182 71 242 1.67e-11 62.8
MS.gene07218.t1 MTR_8g042900 26.667 165 110 4 33 189 76 237 2.09e-11 62.4
MS.gene07218.t1 MTR_2g436040 25.301 166 108 4 28 186 38 194 2.93e-11 62.0
MS.gene07218.t1 MTR_4g124550 36.170 94 58 1 95 188 142 233 4.13e-11 61.6
MS.gene07218.t1 MTR_7g108905 27.673 159 105 2 34 186 46 200 7.44e-11 60.8
MS.gene07218.t1 MTR_1g083510 25.000 156 113 3 33 187 56 208 9.13e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07218.t1 AT3G62820 47.619 189 95 2 6 191 7 194 1.41e-56 177
MS.gene07218.t1 AT2G47670 48.447 161 80 2 33 191 49 208 2.24e-38 131
MS.gene07218.t1 AT5G20740 35.979 189 112 3 10 189 8 196 6.75e-31 112
MS.gene07218.t1 AT5G20740 35.979 189 112 3 10 189 42 230 1.91e-30 112
MS.gene07218.t1 AT4G12390 37.736 159 92 2 36 187 43 201 1.38e-29 108
MS.gene07218.t1 AT1G62760 33.951 162 104 1 31 189 146 307 1.74e-29 111
MS.gene07218.t1 AT5G62360 35.443 158 99 2 33 188 44 200 4.02e-29 107
MS.gene07218.t1 AT5G62350 34.356 163 101 2 33 189 39 201 4.23e-29 107
MS.gene07218.t1 AT1G14890 32.474 194 113 4 10 190 30 218 3.03e-27 103
MS.gene07218.t1 AT2G01610 34.444 180 96 4 31 190 43 220 6.36e-27 102
MS.gene07218.t1 AT4G25260 30.256 195 120 5 10 188 5 199 1.13e-26 101
MS.gene07218.t1 AT3G47380 34.783 161 99 2 33 187 39 199 5.43e-26 99.4
MS.gene07218.t1 AT1G70720 33.505 194 115 4 8 191 9 198 5.88e-25 96.7
MS.gene07218.t1 AT1G70720 33.505 194 115 4 8 191 21 210 6.12e-25 97.1
MS.gene07218.t1 AT1G23205 34.737 190 111 4 8 185 10 198 2.15e-24 95.5
MS.gene07218.t1 AT1G62770 31.250 160 102 4 36 187 37 196 3.28e-23 92.4
MS.gene07218.t1 AT4G25250 30.128 156 104 4 33 183 38 193 9.58e-19 80.5
MS.gene07218.t1 AT3G14310 33.333 168 106 4 33 195 59 225 7.06e-18 81.3
MS.gene07218.t1 AT5G51520 24.752 202 132 5 5 186 1 202 1.85e-16 74.7
MS.gene07218.t1 AT4G00080 30.723 166 103 5 30 184 33 197 4.36e-15 70.9
MS.gene07218.t1 AT1G53840 29.143 175 109 5 32 196 72 241 1.79e-14 71.6
MS.gene07218.t1 AT1G53830 29.940 167 113 3 33 195 62 228 9.47e-14 69.3
MS.gene07218.t1 AT5G04960 30.120 166 99 4 32 196 67 216 1.31e-13 68.9
MS.gene07218.t1 AT4G15980 27.815 151 105 2 37 186 52 199 2.12e-13 68.6
MS.gene07218.t1 AT4G15980 27.815 151 105 2 37 186 62 209 2.41e-13 68.2
MS.gene07218.t1 AT3G05610 25.806 155 113 2 33 187 58 210 5.97e-13 67.0
MS.gene07218.t1 AT3G14300 26.946 167 110 4 32 187 270 435 4.62e-12 64.7
MS.gene07218.t1 AT5G49180 28.205 156 110 2 33 187 55 209 7.68e-12 63.9
MS.gene07218.t1 AT5G27870 25.455 165 120 3 33 197 57 218 1.20e-11 63.2
MS.gene07218.t1 AT3G49220 33.077 130 80 3 28 156 127 250 1.35e-11 62.8
MS.gene07218.t1 AT3G49220 33.077 130 80 3 28 156 127 250 3.02e-11 62.0
MS.gene07218.t1 AT3G05620 25.434 173 112 4 30 186 30 201 4.02e-11 61.6
MS.gene07218.t1 AT3G49220 33.077 130 80 3 28 156 82 205 4.99e-11 61.2

Find 38 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAGAGCTAGAGCGTTAC+TGG 0.280918 1.4:+70290556 None:intergenic
GATCAAGATACTTGCCTTGA+TGG 0.282001 1.4:-70290647 MS.gene07218:CDS
CCAGCCCTTGCCCACTTGCT+AGG 0.314770 1.4:+70290902 None:intergenic
CTGCACTTATCCATGTATTA+AGG 0.338152 1.4:+70290678 None:intergenic
AGCGTTACTGGTTATATAAT+AGG 0.353010 1.4:+70290568 None:intergenic
ACTTGCCTTGATGGATTTCA+AGG 0.400968 1.4:-70290638 MS.gene07218:CDS
CAGCCCTTGCCCACTTGCTA+GGG 0.415413 1.4:+70290903 None:intergenic
AGTCAGTTGGTATTTGCAAA+AGG 0.416317 1.4:-70291019 MS.gene07218:CDS
ACTAGACACGGGCATTTAAG+AGG 0.436602 1.4:-70290824 MS.gene07218:CDS
CCTAGCAAGTGGGCAAGGGC+TGG 0.454457 1.4:-70290902 MS.gene07218:CDS
GTTAATAAACTTGCCACTAC+AGG 0.455754 1.4:-70290536 MS.gene07218:CDS
ACCAACTGACTTGCAAGGTA+TGG 0.470021 1.4:+70291031 None:intergenic
TTAAGTTTGCGAGATATGCT+TGG 0.470762 1.4:+70290846 None:intergenic
AGGCTAAGTAGAACGACATT+TGG 0.472627 1.4:-70290716 MS.gene07218:CDS
ACGACATTTGGTACCCAGAT+GGG 0.493858 1.4:-70290704 MS.gene07218:CDS
GAAGCCCTAGCAAGTGGGCA+AGG 0.500888 1.4:-70290907 MS.gene07218:CDS
GTCAGTTGGTATTTGCAAAA+GGG 0.511286 1.4:-70291018 MS.gene07218:CDS
CTCGCAAACTTAACTAGACA+CGG 0.515036 1.4:-70290836 MS.gene07218:CDS
GAGAAGCTTGCAAAGTAACT+AGG 0.515953 1.4:-70290982 MS.gene07218:CDS
TCCATACCTTGCAAGTCAGT+TGG 0.518339 1.4:-70291032 MS.gene07218:CDS
AAACTTGCCACTACAGGTCT+AGG 0.519005 1.4:-70290530 MS.gene07218:CDS
GCACCGTTCTCTAACAGTGC+TGG 0.521547 1.4:-70290932 MS.gene07218:CDS
GTGATCTATGAAGCTCATCA+AGG 0.523992 1.4:+70290753 None:intergenic
GATCACTGAATGTGCTCAGA+AGG 0.546424 1.4:-70290736 MS.gene07218:CDS
GCTGGAGTGTCAGTGACAAT+CGG 0.557488 1.4:-70290884 MS.gene07218:CDS
CAAATACCAACTGACTTGCA+AGG 0.559475 1.4:+70291026 None:intergenic
AACGACATTTGGTACCCAGA+TGG 0.584170 1.4:-70290705 MS.gene07218:CDS
CGACATTTGGTACCCAGATG+GGG 0.585386 1.4:-70290703 MS.gene07218:CDS
TGATCTATGAAGCTCATCAA+GGG 0.595202 1.4:+70290754 None:intergenic
CTGGAAGAAGCCCTAGCAAG+TGG 0.599584 1.4:-70290913 MS.gene07218:CDS
CTTCCAGCACTGTTAGAGAA+CGG 0.607318 1.4:+70290929 None:intergenic
AAGCCCTAGCAAGTGGGCAA+GGG 0.611205 1.4:-70290906 MS.gene07218:CDS
GATGCTTCCTAGACCTGTAG+TGG 0.614161 1.4:+70290523 None:intergenic
GTGCTAGCGAGCGCGTACAA+AGG 0.625584 1.4:+70290951 None:intergenic
TAAATGCAAGCAGAAAGGTA+AGG 0.639842 1.4:+70291062 None:intergenic
GACATTTGGTACCCAGATGG+GGG 0.662357 1.4:-70290702 MS.gene07218:CDS
TGGAAGAAGCCCTAGCAAGT+GGG 0.667217 1.4:-70290912 MS.gene07218:CDS
TCGCAAACTTAACTAGACAC+GGG 0.670532 1.4:-70290835 MS.gene07218:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAATAAATGCAAGCAGAA+AGG + chr1.4:70290539-70290558 None:intergenic 25.0%
! AGCGTTACTGGTTATATAAT+AGG + chr1.4:70291028-70291047 None:intergenic 30.0%
CTGCACTTATCCATGTATTA+AGG + chr1.4:70290918-70290937 None:intergenic 35.0%
GATTTCAAGGCAAAAACAGT+AGG - chr1.4:70290968-70290987 MS.gene07218:CDS 35.0%
GTTAATAAACTTGCCACTAC+AGG - chr1.4:70291057-70291076 MS.gene07218:CDS 35.0%
TAAATGCAAGCAGAAAGGTA+AGG + chr1.4:70290534-70290553 None:intergenic 35.0%
TGATCTATGAAGCTCATCAA+GGG + chr1.4:70290842-70290861 None:intergenic 35.0%
TTAAGTTTGCGAGATATGCT+TGG + chr1.4:70290750-70290769 None:intergenic 35.0%
! AGTCAGTTGGTATTTGCAAA+AGG - chr1.4:70290574-70290593 MS.gene07218:CDS 35.0%
! GTCAGTTGGTATTTGCAAAA+GGG - chr1.4:70290575-70290594 MS.gene07218:CDS 35.0%
! TTTTGCCTTGAAATCCATCA+AGG + chr1.4:70290963-70290982 None:intergenic 35.0%
AGGCTAAGTAGAACGACATT+TGG - chr1.4:70290877-70290896 MS.gene07218:CDS 40.0%
CTCGCAAACTTAACTAGACA+CGG - chr1.4:70290757-70290776 MS.gene07218:CDS 40.0%
GAGAAGCTTGCAAAGTAACT+AGG - chr1.4:70290611-70290630 MS.gene07218:CDS 40.0%
GATCAAGATACTTGCCTTGA+TGG - chr1.4:70290946-70290965 MS.gene07218:CDS 40.0%
GTGATCTATGAAGCTCATCA+AGG + chr1.4:70290843-70290862 None:intergenic 40.0%
TCGCAAACTTAACTAGACAC+GGG - chr1.4:70290758-70290777 MS.gene07218:CDS 40.0%
!! ACTTGCCTTGATGGATTTCA+AGG - chr1.4:70290955-70290974 MS.gene07218:CDS 40.0%
!! CAAATACCAACTGACTTGCA+AGG + chr1.4:70290570-70290589 None:intergenic 40.0%
AAACTTGCCACTACAGGTCT+AGG - chr1.4:70291063-70291082 MS.gene07218:CDS 45.0%
AACGACATTTGGTACCCAGA+TGG - chr1.4:70290888-70290907 MS.gene07218:CDS 45.0%
ACGACATTTGGTACCCAGAT+GGG - chr1.4:70290889-70290908 MS.gene07218:CDS 45.0%
ACTAGACACGGGCATTTAAG+AGG - chr1.4:70290769-70290788 MS.gene07218:CDS 45.0%
AGATGGGGGACCTTAATACA+TGG - chr1.4:70290905-70290924 MS.gene07218:CDS 45.0%
ATGTATTAAGGTCCCCCATC+TGG + chr1.4:70290906-70290925 None:intergenic 45.0%
CTTCCAGCACTGTTAGAGAA+CGG + chr1.4:70290667-70290686 None:intergenic 45.0%
GATCACTGAATGTGCTCAGA+AGG - chr1.4:70290857-70290876 MS.gene07218:CDS 45.0%
TCCATACCTTGCAAGTCAGT+TGG - chr1.4:70290561-70290580 MS.gene07218:CDS 45.0%
TGTATTAAGGTCCCCCATCT+GGG + chr1.4:70290905-70290924 None:intergenic 45.0%
! AACAAGAGCTAGAGCGTTAC+TGG + chr1.4:70291040-70291059 None:intergenic 45.0%
!! ACCAACTGACTTGCAAGGTA+TGG + chr1.4:70290565-70290584 None:intergenic 45.0%
CGACATTTGGTACCCAGATG+GGG - chr1.4:70290890-70290909 MS.gene07218:CDS 50.0%
GACATTTGGTACCCAGATGG+GGG - chr1.4:70290891-70290910 MS.gene07218:CDS 50.0%
GATGCTTCCTAGACCTGTAG+TGG + chr1.4:70291073-70291092 None:intergenic 50.0%
TGGAAGAAGCCCTAGCAAGT+GGG - chr1.4:70290681-70290700 MS.gene07218:CDS 50.0%
! GCTGGAGTGTCAGTGACAAT+CGG - chr1.4:70290709-70290728 MS.gene07218:CDS 50.0%
AAGCCCTAGCAAGTGGGCAA+GGG - chr1.4:70290687-70290706 MS.gene07218:CDS 55.0%
CTGGAAGAAGCCCTAGCAAG+TGG - chr1.4:70290680-70290699 MS.gene07218:CDS 55.0%
GCACCGTTCTCTAACAGTGC+TGG - chr1.4:70290661-70290680 MS.gene07218:CDS 55.0%
GAAGCCCTAGCAAGTGGGCA+AGG - chr1.4:70290686-70290705 MS.gene07218:CDS 60.0%
GTGCTAGCGAGCGCGTACAA+AGG + chr1.4:70290645-70290664 None:intergenic 60.0%
! CAGCCCTTGCCCACTTGCTA+GGG + chr1.4:70290693-70290712 None:intergenic 60.0%
CCAGCCCTTGCCCACTTGCT+AGG + chr1.4:70290694-70290713 None:intergenic 65.0%
CCTAGCAAGTGGGCAAGGGC+TGG - chr1.4:70290691-70290710 MS.gene07218:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 70290511 70291104 70290511 ID=MS.gene07218
chr1.4 mRNA 70290511 70291104 70290511 ID=MS.gene07218.t1;Parent=MS.gene07218
chr1.4 exon 70290511 70291104 70290511 ID=MS.gene07218.t1.exon1;Parent=MS.gene07218.t1
chr1.4 CDS 70290511 70291104 70290511 ID=cds.MS.gene07218.t1;Parent=MS.gene07218.t1
Gene Sequence

>MS.gene07218

ATGGTCAGAAGAATATCACACCTTACCTTTCTGCTTGCATTTATTTTTATTCCATACCTTGCAAGTCAGTTGGTATTTGCAAAAGGGAAAGACAATGTTAGAGAAGCTTGCAAAGTAACTAGGTATCAAAACCTTTGTACGCGCTCGCTAGCACCGTTCTCTAACAGTGCTGGAAGAAGCCCTAGCAAGTGGGCAAGGGCTGGAGTGTCAGTGACAATCGGTGAAGTTAAGAATGTCCAAGCATATCTCGCAAACTTAACTAGACACGGGCATTTAAGAGGAAGAAATAAAGTTGCATTATTAGATTGTGTCGAGACTATTGCAGATGCCCTTGATGAGCTTCATAGATCACTGAATGTGCTCAGAAGGCTAAGTAGAACGACATTTGGTACCCAGATGGGGGACCTTAATACATGGATAAGTGCAGCACTCACAGATCAAGATACTTGCCTTGATGGATTTCAAGGCAAAAACAGTAGGACAATTCAATTGCTGCAAAAGAGAGTTCTCAAAGCCTATTATATAACCAGTAACGCTCTAGCTCTTGTTAATAAACTTGCCACTACAGGTCTAGGAAGCATCTCTGATCCATAG

Protein sequence

>MS.gene07218.t1

MVRRISHLTFLLAFIFIPYLASQLVFAKGKDNVREACKVTRYQNLCTRSLAPFSNSAGRSPSKWARAGVSVTIGEVKNVQAYLANLTRHGHLRGRNKVALLDCVETIADALDELHRSLNVLRRLSRTTFGTQMGDLNTWISAALTDQDTCLDGFQGKNSRTIQLLQKRVLKAYYITSNALALVNKLATTGLGSISDP