Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073720.t1 | XP_003597137.2 | 92.3 | 65 | 5 | 0 | 1 | 65 | 279 | 343 | 1.50E-27 | 132.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073720.t1 | A0A072VME8 | 92.3 | 65 | 5 | 0 | 1 | 65 | 279 | 343 | 1.1e-27 | 132.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene073720 | 0.802198 | 6.29E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene073720 | 0.821558 | 3.79E-53 | -1.69E-46 |
| MS.gene049254 | MS.gene073720 | 0.810172 | 1.32E-50 | -1.69E-46 |
| MS.gene049404 | MS.gene073720 | 0.818892 | 1.55E-52 | -1.69E-46 |
| MS.gene050273 | MS.gene073720 | 0.800146 | 1.65E-48 | -1.69E-46 |
| MS.gene050826 | MS.gene073720 | 0.818528 | 1.87E-52 | -1.69E-46 |
| MS.gene051181 | MS.gene073720 | 0.82322 | 1.56E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene073720 | 0.817859 | 2.65E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene073720 | 0.801903 | 7.23E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene073720 | 0.818374 | 2.03E-52 | -1.69E-46 |
| MS.gene051800 | MS.gene073720 | 0.822189 | 2.71E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene073720 | 0.801667 | 8.08E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene073720 | 0.826957 | 2.04E-54 | -1.69E-46 |
| MS.gene052773 | MS.gene073720 | 0.801563 | 8.49E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene073720 | 0.801794 | 7.61E-49 | -1.69E-46 |
| MS.gene053883 | MS.gene073720 | 0.810452 | 1.14E-50 | -1.69E-46 |
| MS.gene05415 | MS.gene073720 | 0.811407 | 7.11E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene073720 | 0.81608 | 6.65E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene073720 | 0.822056 | 2.90E-53 | -1.69E-46 |
| MS.gene055227 | MS.gene073720 | 0.820797 | 5.67E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene073720 | 0.809172 | 2.16E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene073720 | 0.821541 | 3.82E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene073720 | 0.826786 | 2.24E-54 | -1.69E-46 |
| MS.gene056050 | MS.gene073720 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene056174 | MS.gene073720 | 0.841905 | 3.58E-58 | -1.69E-46 |
| MS.gene057444 | MS.gene073720 | 0.814761 | 1.31E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene073720 | 0.803705 | 3.07E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene073720 | 0.822633 | 2.13E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene073720 | 0.810975 | 8.82E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene073720 | 0.803296 | 3.73E-49 | -1.69E-46 |
| MS.gene060011 | MS.gene073720 | 0.819767 | 9.77E-53 | -1.69E-46 |
| MS.gene060167 | MS.gene073720 | 0.800894 | 1.16E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene073720 | 0.814603 | 1.42E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene073720 | 0.808434 | 3.10E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073720.t1 | MTR_2g093060 | 92.308 | 65 | 5 | 0 | 1 | 65 | 264 | 328 | 8.44e-38 | 132 |
| MS.gene073720.t1 | MTR_2g093060 | 92.308 | 65 | 5 | 0 | 1 | 65 | 279 | 343 | 8.50e-38 | 132 |
| MS.gene073720.t1 | MTR_2g093060 | 92.308 | 65 | 5 | 0 | 1 | 65 | 279 | 343 | 8.88e-38 | 132 |
| MS.gene073720.t1 | MTR_6g021760 | 95.122 | 41 | 2 | 0 | 63 | 103 | 213 | 253 | 2.67e-20 | 83.6 |
| MS.gene073720.t1 | MTR_4g061200 | 60.000 | 65 | 26 | 0 | 1 | 65 | 261 | 325 | 3.30e-19 | 80.9 |
| MS.gene073720.t1 | MTR_4g061200 | 60.000 | 65 | 26 | 0 | 1 | 65 | 276 | 340 | 3.56e-19 | 80.9 |
| MS.gene073720.t1 | MTR_4g061200 | 60.000 | 65 | 26 | 0 | 1 | 65 | 276 | 340 | 1.10e-18 | 80.1 |
| MS.gene073720.t1 | MTR_4g061200 | 61.905 | 63 | 24 | 0 | 1 | 63 | 261 | 323 | 1.14e-18 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAATCTTGACCTTAATT+TGG | 0.121056 | 1.1:-36784452 | MS.gene073720:CDS |
| ACTCGACCCTTGCAGGTATT+TGG | 0.277646 | 1.1:-36783790 | MS.gene073720:intron |
| CACAATCTTGACCTTAATTT+GGG | 0.311164 | 1.1:-36784451 | MS.gene073720:CDS |
| GCATCCTGGAAGGAATTTAA+AGG | 0.321244 | 1.1:-36784353 | MS.gene073720:intron |
| TATACCTTTAAATTCCTTCC+AGG | 0.328073 | 1.1:+36784349 | None:intergenic |
| CAGCTATAGGCTTTGTTTGA+GGG | 0.381118 | 1.1:+36783959 | None:intergenic |
| GAGTTGCCATGCCCAAATTA+AGG | 0.395598 | 1.1:+36784440 | None:intergenic |
| TTAGCATTAGCAGCAGCTAT+AGG | 0.414591 | 1.1:+36783946 | None:intergenic |
| TAGAAGATTGTCTGACAGTT+CGG | 0.435587 | 1.1:+36783912 | None:intergenic |
| GCAGCTATAGGCTTTGTTTG+AGG | 0.446945 | 1.1:+36783958 | None:intergenic |
| CTACCTTCATTAATAGCTCC+TGG | 0.455299 | 1.1:+36784616 | None:intergenic |
| TTAAATTCCTTCCAGGATGC+AGG | 0.456036 | 1.1:+36784356 | None:intergenic |
| CCTTCTTACAACCTGCATCC+TGG | 0.478916 | 1.1:-36784367 | MS.gene073720:CDS |
| CGACCCTTGCAGGTATTTGG+CGG | 0.507507 | 1.1:-36783787 | MS.gene073720:intron |
| CCAGGATGCAGGTTGTAAGA+AGG | 0.508939 | 1.1:+36784367 | None:intergenic |
| AATTTGGGCATGGCAACTCC+AGG | 0.511024 | 1.1:-36784436 | MS.gene073720:CDS |
| TCTTGACCTTAATTTGGGCA+TGG | 0.552071 | 1.1:-36784446 | MS.gene073720:CDS |
| AAACCAGGAGCTATTAATGA+AGG | 0.564865 | 1.1:-36784619 | MS.gene073720:intron |
| CAGGATGCAGGTTGTAAGAA+GGG | 0.578545 | 1.1:+36784368 | None:intergenic |
| AACATGTGGAAAGAGTGATT+CGG | 0.599431 | 1.1:-36783987 | MS.gene073720:CDS |
| CTTACAACCTGCATCCTGGA+AGG | 0.620038 | 1.1:-36784363 | MS.gene073720:CDS |
| ATGCAGGTTGTAAGAAGGGA+CGG | 0.626558 | 1.1:+36784372 | None:intergenic |
| GAAGCAGAAGGTTCAACATG+TGG | 0.629269 | 1.1:-36784001 | MS.gene073720:CDS |
| TATGAAAATGAGATGAAACC+AGG | 0.629332 | 1.1:-36784634 | MS.gene073720:CDS |
| CATGAACCCAAAGAAAACAA+AGG | 0.637049 | 1.1:-36784412 | MS.gene073720:CDS |
| TCGCCATGCACAGAAGCAGA+AGG | 0.644498 | 1.1:-36784013 | MS.gene073720:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATTTGCTCAATATATAA+AGG | - | chr1.1:36783933-36783952 | MS.gene073720:CDS | 15.0% |
| !! | ATATTTGCTCAATATATAAA+GGG | - | chr1.1:36783934-36783953 | MS.gene073720:CDS | 15.0% |
| !! | CCAAATTTAAATATATAAGT+AGG | - | chr1.1:36784131-36784150 | MS.gene073720:intron | 15.0% |
| !! | CCTACTTATATATTTAAATT+TGG | + | chr1.1:36784134-36784153 | None:intergenic | 15.0% |
| !! | TGACATATTTACAAAATTAA+AGG | + | chr1.1:36784589-36784608 | None:intergenic | 15.0% |
| !! | TTGAGCAAATATTAATTAAT+AGG | + | chr1.1:36783926-36783945 | None:intergenic | 15.0% |
| !! | AAATATGTCAGAAAATACTT+TGG | + | chr1.1:36783857-36783876 | None:intergenic | 20.0% |
| !!! | GTTTAATGAATTAAAGTATC+TGG | + | chr1.1:36783888-36783907 | None:intergenic | 20.0% |
| ! | AAATAAGCTTAGTGCAAAAA+GGG | + | chr1.1:36784185-36784204 | None:intergenic | 25.0% |
| ! | AATGTGTAATATGTGTATTG+AGG | - | chr1.1:36784375-36784394 | MS.gene073720:CDS | 25.0% |
| ! | ACTTATGTTTGTTTAAACAC+AGG | - | chr1.1:36783957-36783976 | MS.gene073720:CDS | 25.0% |
| !! | ATCTCATTTTCATATGTACT+TGG | + | chr1.1:36783798-36783817 | None:intergenic | 25.0% |
| CAAATAAGCTTAGTGCAAAA+AGG | + | chr1.1:36784186-36784205 | None:intergenic | 30.0% | |
| CACAATCTTGACCTTAATTT+GGG | - | chr1.1:36783987-36784006 | MS.gene073720:CDS | 30.0% | |
| TATACCTTTAAATTCCTTCC+AGG | + | chr1.1:36784092-36784111 | None:intergenic | 30.0% | |
| TATGAAAATGAGATGAAACC+AGG | - | chr1.1:36783804-36783823 | MS.gene073720:intron | 30.0% | |
| TATGTTTGTTTAAACACAGG+TGG | - | chr1.1:36783960-36783979 | MS.gene073720:CDS | 30.0% | |
| TCAACATATGCGAAATCATT+GGG | - | chr1.1:36784606-36784625 | MS.gene073720:intron | 30.0% | |
| TCACAATCTTGACCTTAATT+TGG | - | chr1.1:36783986-36784005 | MS.gene073720:CDS | 30.0% | |
| ! | ATGAATTAAAGTATCTGGTC+TGG | + | chr1.1:36783883-36783902 | None:intergenic | 30.0% |
| ! | TGAATTAAAGTATCTGGTCT+GGG | + | chr1.1:36783882-36783901 | None:intergenic | 30.0% |
| !! | TTTTGTATTTTGCTCTGAAC+TGG | - | chr1.1:36784236-36784255 | MS.gene073720:intron | 30.0% |
| !!! | ACGATATCCTTTGTTTTCTT+TGG | + | chr1.1:36784036-36784055 | None:intergenic | 30.0% |
| !!! | CGATATCCTTTGTTTTCTTT+GGG | + | chr1.1:36784035-36784054 | None:intergenic | 30.0% |
| AAACCAGGAGCTATTAATGA+AGG | - | chr1.1:36783819-36783838 | MS.gene073720:intron | 35.0% | |
| AACATGTGGAAAGAGTGATT+CGG | - | chr1.1:36784451-36784470 | MS.gene073720:CDS | 35.0% | |
| CATGAACCCAAAGAAAACAA+AGG | - | chr1.1:36784026-36784045 | MS.gene073720:intron | 35.0% | |
| CCTCGTGAGTAAAGAAAAAT+CGG | + | chr1.1:36784263-36784282 | None:intergenic | 35.0% | |
| GTCAACATATGCGAAATCAT+TGG | - | chr1.1:36784605-36784624 | MS.gene073720:intron | 35.0% | |
| TAGAAGATTGTCTGACAGTT+CGG | + | chr1.1:36784529-36784548 | None:intergenic | 35.0% | |
| CTACCTTCATTAATAGCTCC+TGG | + | chr1.1:36783825-36783844 | None:intergenic | 40.0% | |
| GCAGATAAAATGAACAGAGC+AGG | + | chr1.1:36784341-36784360 | None:intergenic | 40.0% | |
| GCATCCTGGAAGGAATTTAA+AGG | - | chr1.1:36784085-36784104 | MS.gene073720:intron | 40.0% | |
| TTAAATTCCTTCCAGGATGC+AGG | + | chr1.1:36784085-36784104 | None:intergenic | 40.0% | |
| TTAGCATTAGCAGCAGCTAT+AGG | + | chr1.1:36784495-36784514 | None:intergenic | 40.0% | |
| ! | CCGATTTTTCTTTACTCACG+AGG | - | chr1.1:36784260-36784279 | MS.gene073720:intron | 40.0% |
| ! | TCTTGACCTTAATTTGGGCA+TGG | - | chr1.1:36783992-36784011 | MS.gene073720:CDS | 40.0% |
| !! | CAGCTATAGGCTTTGTTTGA+GGG | + | chr1.1:36784482-36784501 | None:intergenic | 40.0% |
| !! | TTTTCTTTGGGTTCATGTCC+TGG | + | chr1.1:36784023-36784042 | None:intergenic | 40.0% |
| ATGCAGGTTGTAAGAAGGGA+CGG | + | chr1.1:36784069-36784088 | None:intergenic | 45.0% | |
| CAGGATGCAGGTTGTAAGAA+GGG | + | chr1.1:36784073-36784092 | None:intergenic | 45.0% | |
| GAAGCAGAAGGTTCAACATG+TGG | - | chr1.1:36784437-36784456 | MS.gene073720:CDS | 45.0% | |
| GAGTTGCCATGCCCAAATTA+AGG | + | chr1.1:36784001-36784020 | None:intergenic | 45.0% | |
| !! | GCAGCTATAGGCTTTGTTTG+AGG | + | chr1.1:36784483-36784502 | None:intergenic | 45.0% |
| AAATACCTGCAAGGGTCGAG+TGG | + | chr1.1:36784649-36784668 | None:intergenic | 50.0% | |
| AAGCCGCCAAATACCTGCAA+GGG | + | chr1.1:36784657-36784676 | None:intergenic | 50.0% | |
| AATTTGGGCATGGCAACTCC+AGG | - | chr1.1:36784002-36784021 | MS.gene073720:CDS | 50.0% | |
| ACTCGACCCTTGCAGGTATT+TGG | - | chr1.1:36784648-36784667 | MS.gene073720:CDS | 50.0% | |
| ATTATCCACTCGACCCTTGC+AGG | - | chr1.1:36784641-36784660 | MS.gene073720:CDS | 50.0% | |
| CCAGGATGCAGGTTGTAAGA+AGG | + | chr1.1:36784074-36784093 | None:intergenic | 50.0% | |
| CCTTCTTACAACCTGCATCC+TGG | - | chr1.1:36784071-36784090 | MS.gene073720:intron | 50.0% | |
| CTTACAACCTGCATCCTGGA+AGG | - | chr1.1:36784075-36784094 | MS.gene073720:intron | 50.0% | |
| GAACCTTCTGCTTCTGTGCA+TGG | + | chr1.1:36784431-36784450 | None:intergenic | 50.0% | |
| CAAGCCGCCAAATACCTGCA+AGG | + | chr1.1:36784658-36784677 | None:intergenic | 55.0% | |
| CGACCCTTGCAGGTATTTGG+CGG | - | chr1.1:36784651-36784670 | MS.gene073720:CDS | 55.0% | |
| TCGCCATGCACAGAAGCAGA+AGG | - | chr1.1:36784425-36784444 | MS.gene073720:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 36783780 | 36784680 | 36783780 | ID=MS.gene073720 |
| chr1.1 | mRNA | 36783780 | 36784680 | 36783780 | ID=MS.gene073720.t1;Parent=MS.gene073720 |
| chr1.1 | exon | 36784620 | 36784680 | 36784620 | ID=MS.gene073720.t1.exon1;Parent=MS.gene073720.t1 |
| chr1.1 | CDS | 36784620 | 36784680 | 36784620 | ID=cds.MS.gene073720.t1;Parent=MS.gene073720.t1 |
| chr1.1 | exon | 36784354 | 36784481 | 36784354 | ID=MS.gene073720.t1.exon2;Parent=MS.gene073720.t1 |
| chr1.1 | CDS | 36784354 | 36784481 | 36784354 | ID=cds.MS.gene073720.t1;Parent=MS.gene073720.t1 |
| chr1.1 | exon | 36783906 | 36784022 | 36783906 | ID=MS.gene073720.t1.exon3;Parent=MS.gene073720.t1 |
| chr1.1 | CDS | 36783906 | 36784022 | 36783906 | ID=cds.MS.gene073720.t1;Parent=MS.gene073720.t1 |
| chr1.1 | exon | 36783780 | 36783797 | 36783780 | ID=MS.gene073720.t1.exon4;Parent=MS.gene073720.t1 |
| chr1.1 | CDS | 36783780 | 36783797 | 36783780 | ID=cds.MS.gene073720.t1;Parent=MS.gene073720.t1 |
| Gene Sequence |
| Protein sequence |
