Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08052.t1 | XP_003600461.1 | 99.4 | 313 | 2 | 0 | 1 | 313 | 1 | 313 | 4.00E-185 | 657.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08052.t1 | Q9FKT5 | 85.0 | 313 | 46 | 1 | 2 | 313 | 3 | 315 | 1.7e-160 | 566.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08052.t1 | G7IY98 | 99.4 | 313 | 2 | 0 | 1 | 313 | 1 | 313 | 2.9e-185 | 657.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08052 | MS.gene014281 | PPI |
| MS.gene08052 | MS.gene93058 | PPI |
| MS.gene064894 | MS.gene08052 | PPI |
| MS.gene33535 | MS.gene08052 | PPI |
| MS.gene066026 | MS.gene08052 | PPI |
| MS.gene056487 | MS.gene08052 | PPI |
| MS.gene08052 | MS.gene012608 | PPI |
| MS.gene08052 | MS.gene004825 | PPI |
| MS.gene08052 | MS.gene71431 | PPI |
| MS.gene08052 | MS.gene042253 | PPI |
| MS.gene08052 | MS.gene68222 | PPI |
| MS.gene52980 | MS.gene08052 | PPI |
| MS.gene046235 | MS.gene08052 | PPI |
| MS.gene08052 | MS.gene97980 | PPI |
| MS.gene056488 | MS.gene08052 | PPI |
| MS.gene08052 | MS.gene008793 | PPI |
| MS.gene93059 | MS.gene08052 | PPI |
| MS.gene08052 | MS.gene008794 | PPI |
| MS.gene08052 | MS.gene063165 | PPI |
| MS.gene95244 | MS.gene08052 | PPI |
| MS.gene97981 | MS.gene08052 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08052.t1 | MTR_3g061450 | 99.361 | 313 | 2 | 0 | 1 | 313 | 1 | 313 | 0.0 | 654 |
| MS.gene08052.t1 | MTR_4g095058 | 27.835 | 291 | 191 | 7 | 11 | 289 | 280 | 563 | 5.03e-21 | 93.6 |
| MS.gene08052.t1 | MTR_4g095058 | 28.962 | 183 | 109 | 5 | 8 | 177 | 402 | 576 | 1.11e-14 | 74.7 |
| MS.gene08052.t1 | MTR_4g095058 | 27.835 | 291 | 191 | 7 | 11 | 289 | 278 | 561 | 5.30e-21 | 93.6 |
| MS.gene08052.t1 | MTR_4g095058 | 28.962 | 183 | 109 | 5 | 8 | 177 | 400 | 574 | 1.09e-14 | 74.7 |
| MS.gene08052.t1 | MTR_5g016350 | 24.925 | 333 | 203 | 9 | 17 | 307 | 231 | 558 | 2.07e-17 | 83.2 |
| MS.gene08052.t1 | MTR_5g016350 | 29.775 | 178 | 104 | 5 | 13 | 177 | 412 | 581 | 2.12e-15 | 77.0 |
| MS.gene08052.t1 | MTR_5g016350 | 24.823 | 282 | 153 | 9 | 20 | 249 | 295 | 569 | 3.72e-13 | 70.1 |
| MS.gene08052.t1 | MTR_5g016350 | 24.925 | 333 | 203 | 9 | 17 | 307 | 167 | 494 | 2.49e-17 | 82.8 |
| MS.gene08052.t1 | MTR_5g016350 | 29.775 | 178 | 104 | 5 | 13 | 177 | 348 | 517 | 3.62e-15 | 76.3 |
| MS.gene08052.t1 | MTR_5g016350 | 24.823 | 282 | 153 | 9 | 20 | 249 | 231 | 505 | 4.36e-13 | 70.1 |
| MS.gene08052.t1 | MTR_5g016370 | 24.405 | 336 | 199 | 11 | 17 | 307 | 245 | 570 | 2.40e-16 | 79.7 |
| MS.gene08052.t1 | MTR_5g016370 | 28.409 | 176 | 109 | 4 | 13 | 177 | 424 | 593 | 7.29e-16 | 78.6 |
| MS.gene08052.t1 | MTR_4g125710 | 24.627 | 268 | 176 | 7 | 13 | 258 | 54 | 317 | 5.13e-16 | 77.8 |
| MS.gene08052.t1 | MTR_1g072140 | 23.311 | 296 | 211 | 9 | 11 | 297 | 19 | 307 | 9.33e-16 | 77.0 |
| MS.gene08052.t1 | MTR_1g097140 | 25.769 | 260 | 181 | 6 | 12 | 267 | 116 | 367 | 1.27e-15 | 77.4 |
| MS.gene08052.t1 | MTR_3g106080 | 25.882 | 255 | 124 | 6 | 15 | 251 | 457 | 664 | 4.42e-15 | 76.3 |
| MS.gene08052.t1 | MTR_3g449990 | 25.000 | 288 | 202 | 9 | 17 | 295 | 268 | 550 | 3.32e-14 | 73.6 |
| MS.gene08052.t1 | MTR_4g119620 | 25.203 | 246 | 171 | 7 | 13 | 251 | 72 | 311 | 1.13e-13 | 70.9 |
| MS.gene08052.t1 | MTR_5g090420 | 24.739 | 287 | 201 | 9 | 18 | 295 | 263 | 543 | 1.20e-13 | 71.6 |
| MS.gene08052.t1 | MTR_7g086360 | 21.141 | 298 | 218 | 7 | 16 | 301 | 52 | 344 | 1.68e-13 | 70.5 |
| MS.gene08052.t1 | MTR_6g004040 | 27.160 | 243 | 158 | 8 | 13 | 244 | 5 | 239 | 1.53e-12 | 68.6 |
| MS.gene08052.t1 | MTR_5g022690 | 26.066 | 211 | 142 | 7 | 13 | 217 | 273 | 475 | 2.63e-11 | 64.3 |
| MS.gene08052.t1 | MTR_1g069345 | 23.510 | 302 | 209 | 9 | 7 | 294 | 204 | 497 | 3.06e-11 | 64.3 |
| MS.gene08052.t1 | MTR_2g016160 | 31.852 | 135 | 77 | 5 | 13 | 140 | 101 | 227 | 4.69e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08052.t1 | AT5G56130 | 85.079 | 315 | 45 | 2 | 1 | 313 | 1 | 315 | 0.0 | 566 |
| MS.gene08052.t1 | AT5G67320 | 28.669 | 293 | 186 | 10 | 11 | 289 | 315 | 598 | 1.39e-22 | 98.6 |
| MS.gene08052.t1 | AT5G67320 | 29.143 | 175 | 111 | 4 | 12 | 177 | 441 | 611 | 2.87e-14 | 73.6 |
| MS.gene08052.t1 | AT2G43770 | 23.729 | 295 | 208 | 8 | 16 | 298 | 50 | 339 | 1.21e-16 | 79.7 |
| MS.gene08052.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 5.52e-15 | 75.9 |
| MS.gene08052.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 5.52e-15 | 75.9 |
| MS.gene08052.t1 | AT5G08560 | 25.260 | 289 | 196 | 9 | 31 | 311 | 286 | 562 | 5.52e-15 | 75.9 |
| MS.gene08052.t1 | AT5G25150 | 25.292 | 257 | 123 | 7 | 15 | 251 | 456 | 663 | 3.88e-14 | 73.6 |
| MS.gene08052.t1 | AT3G49660 | 24.671 | 304 | 200 | 11 | 10 | 295 | 15 | 307 | 5.32e-14 | 72.0 |
| MS.gene08052.t1 | AT3G49660 | 23.715 | 253 | 180 | 7 | 13 | 258 | 65 | 311 | 2.06e-12 | 67.0 |
| MS.gene08052.t1 | AT5G25150 | 27.143 | 210 | 100 | 6 | 15 | 220 | 456 | 616 | 5.93e-13 | 69.7 |
| MS.gene08052.t1 | AT4G02730 | 26.415 | 212 | 142 | 8 | 14 | 217 | 80 | 285 | 2.19e-12 | 67.4 |
Find 74 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCAAGTACATTGTATTT+AGG | 0.212077 | 3.3:-49051014 | None:intergenic |
| TTGATCAAACTGCTCGAATT+TGG | 0.221602 | 3.3:+49044324 | MS.gene08052:CDS |
| CTCATGTAGCTGTCGGTAAT+AGG | 0.263264 | 3.3:+49045639 | MS.gene08052:CDS |
| ACAATTAGTTTCAATCATAC+TGG | 0.283022 | 3.3:+49050418 | MS.gene08052:CDS |
| TGCAATAAGATCGGGATGTT+TGG | 0.289165 | 3.3:-49045329 | None:intergenic |
| GCAATAAGATCGGGATGTTT+GGG | 0.297004 | 3.3:-49045328 | None:intergenic |
| GTGACAAGACTGTTCGTCTA+TGG | 0.297252 | 3.3:+49045362 | MS.gene08052:CDS |
| CGATCTTATTGCAACTGCTT+CGG | 0.300112 | 3.3:+49045339 | MS.gene08052:CDS |
| TGTCAGCTTGTGGGTTATTT+CGG | 0.322981 | 3.3:+49050239 | MS.gene08052:CDS |
| GATCTTATTGCAACTGCTTC+GGG | 0.329060 | 3.3:+49045340 | MS.gene08052:CDS |
| TTCTTTCTAACAACAGGAAA+TGG | 0.391405 | 3.3:+49049840 | MS.gene08052:CDS |
| CTGATTCCCTTGTCAGCTTG+TGG | 0.395348 | 3.3:+49050229 | MS.gene08052:CDS |
| TGACAGTCCAATGTTCATAC+TGG | 0.402914 | 3.3:+49050936 | MS.gene08052:intron |
| TGATTCCCTTGTCAGCTTGT+GGG | 0.409663 | 3.3:+49050230 | MS.gene08052:CDS |
| CAGTACATCACATTCCTTGT+AGG | 0.411919 | 3.3:+49050964 | MS.gene08052:CDS |
| CTTCACAGCAGAGAGTATTC+TGG | 0.419755 | 3.3:+49044082 | MS.gene08052:CDS |
| TGCATTGCAATTGACCCAAC+AGG | 0.430132 | 3.3:+49050030 | MS.gene08052:CDS |
| AATTAACAATACTTGATGTT+CGG | 0.434738 | 3.3:+49045888 | MS.gene08052:CDS |
| AAAAGGTGCATTCAGTGGCT+TGG | 0.441542 | 3.3:+49044267 | MS.gene08052:intron |
| CCTAGGTAAATGAGATTGCT+TGG | 0.445389 | 3.3:+49049799 | MS.gene08052:intron |
| ATTCCACTCAACACTATTCA+TGG | 0.456424 | 3.3:-49050991 | None:intergenic |
| GAGATGTTCTTTCTAACAAC+AGG | 0.457171 | 3.3:+49049834 | MS.gene08052:CDS |
| ATTGGCACAAAACTTGCTTC+TGG | 0.457664 | 3.3:+49044296 | MS.gene08052:CDS |
| ATGAGTACCATCAGGTTTGT+AGG | 0.460015 | 3.3:-49045621 | None:intergenic |
| GGTCAAAGATATTGAATTGA+AGG | 0.467266 | 3.3:+49045270 | MS.gene08052:CDS |
| CAATTAGTTTCAATCATACT+GGG | 0.469646 | 3.3:+49050419 | MS.gene08052:CDS |
| TGAGACAGACATTTCGCTGT+TGG | 0.470477 | 3.3:+49050201 | MS.gene08052:intron |
| TATGATTGAAACTAATTGTC+CGG | 0.470565 | 3.3:-49050414 | None:intergenic |
| TGTTTCTCAGATGGCCTGTC+CGG | 0.480041 | 3.3:+49050395 | MS.gene08052:intron |
| TCATGTAGCTGTCGGTAATA+GGG | 0.491901 | 3.3:+49045640 | MS.gene08052:CDS |
| AGGTGGAAAATGCTCACAAC+AGG | 0.493748 | 3.3:+49045568 | MS.gene08052:intron |
| CTATTCATGGCAGCCCTACA+AGG | 0.501447 | 3.3:-49050978 | None:intergenic |
| CGAAGCAGTTGCAATAAGAT+CGG | 0.502914 | 3.3:-49045338 | None:intergenic |
| CACAACAGGCAGAACTTAGT+GGG | 0.503581 | 3.3:+49045582 | MS.gene08052:CDS |
| GTATCAAGAGGTCGAAGAGA+TGG | 0.505442 | 3.3:-49049979 | None:intergenic |
| TTGAACTTGCGCCTATGCAT+TGG | 0.510346 | 3.3:-49045920 | None:intergenic |
| TGACAAGACTGTTCGTCTAT+GGG | 0.513043 | 3.3:+49045363 | MS.gene08052:CDS |
| AACTTACTCGAGCTTTGTAA+AGG | 0.513452 | 3.3:-49050280 | None:intergenic |
| AAGAAGCATACCTTCCTGTT+GGG | 0.521567 | 3.3:-49050044 | None:intergenic |
| TCAGTGGCTTGGAATTGCAT+TGG | 0.521845 | 3.3:+49044278 | MS.gene08052:CDS |
| AAATGAGAATTGTTCCTCCA+TGG | 0.522481 | 3.3:-49044053 | None:intergenic |
| TCACAACAGGCAGAACTTAG+TGG | 0.530916 | 3.3:+49045581 | MS.gene08052:CDS |
| GAAGCAGTTGCAATAAGATC+GGG | 0.535149 | 3.3:-49045337 | None:intergenic |
| TACAATGTACTTGCATATGC+CGG | 0.539937 | 3.3:+49051020 | MS.gene08052:CDS |
| CAAGAAGCATACCTTCCTGT+TGG | 0.550714 | 3.3:-49050045 | None:intergenic |
| TACTCTCATGGCTCATACAG+CGG | 0.555050 | 3.3:+49049999 | MS.gene08052:CDS |
| ACTCTCATGGCTCATACAGC+GGG | 0.555763 | 3.3:+49050000 | MS.gene08052:CDS |
| TAGTGTGGATCAGCTATGTT+GGG | 0.558977 | 3.3:+49045303 | MS.gene08052:CDS |
| GTCAAAGATATTGAATTGAA+GGG | 0.559756 | 3.3:+49045271 | MS.gene08052:CDS |
| AAATAACCCACAAGCTGACA+AGG | 0.562424 | 3.3:-49050236 | None:intergenic |
| TCGACCTCTTGATACTCTCA+TGG | 0.565472 | 3.3:+49049987 | MS.gene08052:CDS |
| AGTACATCACATTCCTTGTA+GGG | 0.574588 | 3.3:+49050965 | MS.gene08052:CDS |
| ATAGTGTGGATCAGCTATGT+TGG | 0.576839 | 3.3:+49045302 | MS.gene08052:CDS |
| GGAAATTCAAACCAATGCAT+AGG | 0.586683 | 3.3:+49045909 | MS.gene08052:CDS |
| TTGAAACTAATTGTCCGGAC+AGG | 0.589710 | 3.3:-49050409 | None:intergenic |
| ACAGCTACATGAGTACCATC+AGG | 0.590433 | 3.3:-49045629 | None:intergenic |
| AAGGTTACTACATGGGCATG+TGG | 0.595342 | 3.3:-49044356 | None:intergenic |
| TAGGCGCAAGTTCAACTATG+AGG | 0.599831 | 3.3:+49045928 | MS.gene08052:CDS |
| GAAGGGTCACGCTGATAGTG+TGG | 0.599942 | 3.3:+49045288 | MS.gene08052:CDS |
| TTTCGGAGATGCTTTGTGTG+CGG | 0.599990 | 3.3:+49050256 | MS.gene08052:CDS |
| GAGATTGCTTGGAACATGAC+TGG | 0.603670 | 3.3:+49049810 | MS.gene08052:CDS |
| AACATCACCTACAAACCTGA+TGG | 0.607731 | 3.3:+49045614 | MS.gene08052:CDS |
| AGGTTACTACATGGGCATGT+GGG | 0.614543 | 3.3:-49044355 | None:intergenic |
| CAATAAGATCGGGATGTTTG+GGG | 0.614895 | 3.3:-49045327 | None:intergenic |
| AACAAGTATCAGGCTGACGA+AGG | 0.633197 | 3.3:+49051053 | MS.gene08052:CDS |
| GATGGTACTCATGTAGCTGT+CGG | 0.638089 | 3.3:+49045632 | MS.gene08052:CDS |
| GTTTGAAAAGGTGCATTCAG+TGG | 0.648774 | 3.3:+49044262 | MS.gene08052:intron |
| GCTCACCTACTCAGGGACTG+TGG | 0.654605 | 3.3:+49049942 | MS.gene08052:intron |
| CTGCCATGAATAGTGTTGAG+TGG | 0.656405 | 3.3:+49050988 | MS.gene08052:CDS |
| TTGCAATTGACCCAACAGGA+AGG | 0.660962 | 3.3:+49050034 | MS.gene08052:CDS |
| AATAACCCACAAGCTGACAA+GGG | 0.681570 | 3.3:-49050235 | None:intergenic |
| AATTAGTTTCAATCATACTG+GGG | 0.695286 | 3.3:+49050420 | MS.gene08052:CDS |
| TGAGCCATGAGAGTATCAAG+AGG | 0.696186 | 3.3:-49049991 | None:intergenic |
| ACAACAGGCAGAACTTAGTG+GGG | 0.714393 | 3.3:+49045583 | MS.gene08052:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 49044055 | 49051196 | 49044055 | ID=MS.gene08052 |
| chr3.3 | mRNA | 49044055 | 49051196 | 49044055 | ID=MS.gene08052.t1;Parent=MS.gene08052 |
| chr3.3 | exon | 49044055 | 49044117 | 49044055 | ID=MS.gene08052.t1.exon1;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49044055 | 49044117 | 49044055 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49044272 | 49044367 | 49044272 | ID=MS.gene08052.t1.exon2;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49044272 | 49044367 | 49044272 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49045265 | 49045394 | 49045265 | ID=MS.gene08052.t1.exon3;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49045265 | 49045394 | 49045265 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49045570 | 49045661 | 49045570 | ID=MS.gene08052.t1.exon4;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49045570 | 49045661 | 49045570 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49045881 | 49045949 | 49045881 | ID=MS.gene08052.t1.exon5;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49045881 | 49045949 | 49045881 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49049804 | 49049861 | 49049804 | ID=MS.gene08052.t1.exon6;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49049804 | 49049861 | 49049804 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49049956 | 49050055 | 49049956 | ID=MS.gene08052.t1.exon7;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49049956 | 49050055 | 49049956 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49050209 | 49050295 | 49050209 | ID=MS.gene08052.t1.exon8;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49050209 | 49050295 | 49050209 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49050405 | 49050483 | 49050405 | ID=MS.gene08052.t1.exon9;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49050405 | 49050483 | 49050405 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49050942 | 49051074 | 49050942 | ID=MS.gene08052.t1.exon10;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49050942 | 49051074 | 49050942 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| chr3.3 | exon | 49051162 | 49051196 | 49051162 | ID=MS.gene08052.t1.exon11;Parent=MS.gene08052.t1 |
| chr3.3 | CDS | 49051162 | 49051196 | 49051162 | ID=cds.MS.gene08052.t1;Parent=MS.gene08052.t1 |
| Gene Sequence |
| Protein sequence |
