Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08361.t1 | RHN42316.1 | 89.9 | 406 | 40 | 1 | 1 | 405 | 81 | 486 | 7.50E-152 | 547 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08361.t1 | Q9M647 | 67.6 | 407 | 111 | 5 | 1 | 405 | 82 | 469 | 3.7e-99 | 363.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08361.t1 | A0A396GMG8 | 89.9 | 406 | 40 | 1 | 1 | 405 | 81 | 486 | 5.4e-152 | 547.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049824 | MS.gene08361 | 0.853231 | 2.74E-61 | -1.69E-46 |
| MS.gene049825 | MS.gene08361 | 0.85968 | 3.50E-63 | -1.69E-46 |
| MS.gene049873 | MS.gene08361 | 0.837028 | 6.62E-57 | -1.69E-46 |
| MS.gene052133 | MS.gene08361 | 0.813381 | 2.63E-51 | -1.69E-46 |
| MS.gene052239 | MS.gene08361 | -0.803905 | 2.79E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene08361 | 0.805547 | 1.27E-49 | -1.69E-46 |
| MS.gene052376 | MS.gene08361 | 0.80221 | 6.25E-49 | -1.69E-46 |
| MS.gene053294 | MS.gene08361 | 0.860393 | 2.13E-63 | -1.69E-46 |
| MS.gene053599 | MS.gene08361 | 0.827285 | 1.70E-54 | -1.69E-46 |
| MS.gene053623 | MS.gene08361 | 0.81699 | 4.16E-52 | -1.69E-46 |
| MS.gene053678 | MS.gene08361 | 0.81454 | 1.46E-51 | -1.69E-46 |
| MS.gene053995 | MS.gene08361 | 0.815747 | 7.89E-52 | -1.69E-46 |
| MS.gene054222 | MS.gene08361 | 0.814479 | 1.51E-51 | -1.69E-46 |
| MS.gene05548 | MS.gene08361 | 0.805106 | 1.57E-49 | -1.69E-46 |
| MS.gene055555 | MS.gene08361 | 0.802581 | 5.24E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene08361 | 0.838472 | 2.82E-57 | -1.69E-46 |
| MS.gene056091 | MS.gene08361 | 0.855483 | 6.12E-62 | -1.69E-46 |
| MS.gene05795 | MS.gene08361 | 0.81667 | 4.91E-52 | -1.69E-46 |
| MS.gene058074 | MS.gene08361 | 0.804958 | 1.68E-49 | -1.69E-46 |
| MS.gene058635 | MS.gene08361 | 0.849669 | 2.79E-60 | -1.69E-46 |
| MS.gene059317 | MS.gene08361 | 0.801222 | 9.97E-49 | -1.69E-46 |
| MS.gene05953 | MS.gene08361 | 0.818159 | 2.27E-52 | -1.69E-46 |
| MS.gene05954 | MS.gene08361 | 0.832563 | 8.80E-56 | -1.69E-46 |
| MS.gene06001 | MS.gene08361 | 0.825087 | 5.68E-54 | -1.69E-46 |
| MS.gene060619 | MS.gene08361 | 0.81653 | 5.27E-52 | -1.69E-46 |
| MS.gene061266 | MS.gene08361 | 0.814366 | 1.60E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08361.t1 | AT1G68100 | 63.725 | 408 | 125 | 9 | 1 | 405 | 82 | 469 | 8.96e-160 | 458 |
| MS.gene08361.t1 | AT1G68100 | 62.745 | 357 | 110 | 9 | 1 | 354 | 82 | 418 | 9.77e-131 | 383 |
Find 92 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGTGCATTCATTCTTTA+TGG | 0.128139 | 4.1:+17112854 | MS.gene08361:CDS |
| TGCATACAGGTTGGTGATTT+TGG | 0.131631 | 4.1:+17115679 | MS.gene08361:intron |
| GTTGATTCATTGGCTTTATT+TGG | 0.140275 | 4.1:+17110135 | MS.gene08361:CDS |
| TTGATTCATTGGCTTTATTT+GGG | 0.206144 | 4.1:+17110136 | MS.gene08361:CDS |
| ATATTTGACATTTAGGTCTT+TGG | 0.237351 | 4.1:+17109900 | MS.gene08361:intron |
| TCTGCACTTGTGGCACTTGC+AGG | 0.290617 | 4.1:+17115757 | MS.gene08361:CDS |
| CATGGCCATGATGATAATTC+TGG | 0.335638 | 4.1:+17111410 | MS.gene08361:CDS |
| AAAGGCTGTTGTTGATTCAT+TGG | 0.343628 | 4.1:+17110125 | MS.gene08361:CDS |
| CACCAACTACCGCATGCTTT+TGG | 0.343649 | 4.1:+17110444 | MS.gene08361:CDS |
| TTTCAGGCAGGAGCTATGTT+GGG | 0.345216 | 4.1:+17110408 | MS.gene08361:intron |
| GAATCAACAACAGCCTTTGA+TGG | 0.351301 | 4.1:-17110120 | None:intergenic |
| TCTACTGAACTATCGGGTTT+AGG | 0.355559 | 4.1:+17109476 | MS.gene08361:CDS |
| AAATTACAGGCAGGATAATC+AGG | 0.370708 | 4.1:-17109973 | None:intergenic |
| TTTATATACATCGCCATTGC+AGG | 0.389892 | 4.1:+17116412 | MS.gene08361:CDS |
| TTCACTGATGGAATTGCATT+AGG | 0.390135 | 4.1:+17112833 | MS.gene08361:CDS |
| AATTGTACTCTTTCTTCTTG+TGG | 0.396907 | 4.1:+17111951 | MS.gene08361:CDS |
| ATCATTCTGACTACTACCTT+TGG | 0.410847 | 4.1:-17112613 | None:intergenic |
| GAGCATTCCTCACCTCAATC+AGG | 0.413368 | 4.1:-17116308 | None:intergenic |
| TACTGCCTCTACTGAACTAT+CGG | 0.426313 | 4.1:+17109469 | MS.gene08361:CDS |
| TTTATGGATCTGTTGGTGGC+TGG | 0.428280 | 4.1:+17112870 | MS.gene08361:CDS |
| GAGGAAGATATGAAACTCTA+TGG | 0.429877 | 4.1:+17109413 | None:intergenic |
| AAGTTGAAGAACAAAGCTTT+TGG | 0.434106 | 4.1:-17115730 | None:intergenic |
| TTCATTCTTTATGGATCTGT+TGG | 0.436706 | 4.1:+17112863 | MS.gene08361:CDS |
| TGCTATTGTGGTTATGGTGG+TGG | 0.438617 | 4.1:-17112035 | None:intergenic |
| TCTACAGGAATGTCTGTCCT+AGG | 0.440185 | 4.1:+17111464 | MS.gene08361:CDS |
| CCAAACACAAGACTGCTTGC+TGG | 0.440776 | 4.1:-17112696 | None:intergenic |
| CCAGCAAGCAGTCTTGTGTT+TGG | 0.441867 | 4.1:+17112696 | MS.gene08361:CDS |
| TTTGGTATAGGCTCTACTCT+GGG | 0.450056 | 4.1:+17116263 | MS.gene08361:intron |
| ATTCTTTATGGATCTGTTGG+TGG | 0.455219 | 4.1:+17112866 | MS.gene08361:CDS |
| TCTTTAGCTGATCTTTCTAC+AGG | 0.456421 | 4.1:+17111449 | MS.gene08361:CDS |
| CTGTTCATTTCTAGTGAGCA+TGG | 0.462325 | 4.1:+17109937 | MS.gene08361:CDS |
| TTCAGGCAGGAGCTATGTTG+GGG | 0.468392 | 4.1:+17110409 | MS.gene08361:intron |
| GATATGAAACTCTATGGCTT+CGG | 0.473249 | 4.1:+17109419 | None:intergenic |
| GGTCTTTGGTTGAATGCGTT+GGG | 0.474628 | 4.1:+17109914 | MS.gene08361:intron |
| TCAAATAATCTCACTGACAA+TGG | 0.476796 | 4.1:+17116489 | MS.gene08361:CDS |
| AGGTCTTTGGTTGAATGCGT+TGG | 0.479620 | 4.1:+17109913 | MS.gene08361:intron |
| CAAATAATCTCACTGACAAT+GGG | 0.483818 | 4.1:+17116490 | MS.gene08361:CDS |
| ATCTAAATCCTCGCTTAGAA+AGG | 0.488010 | 4.1:+17112200 | MS.gene08361:CDS |
| TGCATGGTCACCATGAGAAT+GGG | 0.491097 | 4.1:-17111384 | None:intergenic |
| AGCGAGGATTTAGATTGAGC+TGG | 0.491576 | 4.1:-17112192 | None:intergenic |
| AGACTGCTTGCTGGTCTAGC+TGG | 0.494277 | 4.1:-17112687 | None:intergenic |
| TTGCAGCATAATTTCACTGA+TGG | 0.495442 | 4.1:+17112821 | MS.gene08361:intron |
| TACCAAAAGCATGCGGTAGT+TGG | 0.508827 | 4.1:-17110446 | None:intergenic |
| CTGCATGGTCACCATGAGAA+TGG | 0.509145 | 4.1:-17111385 | None:intergenic |
| GGCACTTGCAGGCACTGCAT+TGG | 0.512803 | 4.1:+17115768 | MS.gene08361:CDS |
| TCAATCAGGGATGACTGTCC+AGG | 0.514548 | 4.1:-17116294 | None:intergenic |
| ATTCACCTGTTTCAGTTCAA+GGG | 0.517071 | 4.1:+17110094 | MS.gene08361:intron |
| ACTGCCTCTACTGAACTATC+GGG | 0.518695 | 4.1:+17109470 | MS.gene08361:CDS |
| CTCTGCAAGCACTCCTGCAA+TGG | 0.521107 | 4.1:-17116425 | None:intergenic |
| CAGGATAATCAGGCAAATCA+AGG | 0.522978 | 4.1:-17109963 | None:intergenic |
| TGATTCATTGGCTTTATTTG+GGG | 0.541640 | 4.1:+17110137 | MS.gene08361:CDS |
| CATCATGGCCATGGTCTGCA+TGG | 0.544860 | 4.1:-17111400 | None:intergenic |
| TAGAGGCAGTAAAGTGATGA+TGG | 0.549323 | 4.1:-17109457 | None:intergenic |
| TTGCAGAGATGAATAACAAT+GGG | 0.553416 | 4.1:+17116440 | MS.gene08361:CDS |
| TTGGTATAGGCTCTACTCTG+GGG | 0.554436 | 4.1:+17116264 | MS.gene08361:intron |
| CAGAATTATCATCATGGCCA+TGG | 0.554882 | 4.1:-17111409 | None:intergenic |
| ATTCTTGTGTTGCAGAGAAG+TGG | 0.560445 | 4.1:+17112579 | MS.gene08361:intron |
| TGGACAGTCATCCCTGATTG+AGG | 0.566660 | 4.1:+17116296 | MS.gene08361:CDS |
| TCAGGAGGAGCCAACTCGTG+GGG | 0.571716 | 4.1:+17112003 | MS.gene08361:CDS |
| TAAAGTGATGATGGTCGTGA+TGG | 0.572558 | 4.1:-17109448 | None:intergenic |
| TGAAGATTTGACATTATCAG+TGG | 0.572617 | 4.1:-17112652 | None:intergenic |
| GGCACATGAAATTCCTCAAG+AGG | 0.573109 | 4.1:+17112913 | MS.gene08361:CDS |
| TTACAGGGATTTACTGCAGG+TGG | 0.573701 | 4.1:+17116388 | MS.gene08361:intron |
| TCTTGCTATTGTGGTTATGG+TGG | 0.575168 | 4.1:-17112038 | None:intergenic |
| TCAGGCAGGAGCTATGTTGG+GGG | 0.576089 | 4.1:+17110410 | MS.gene08361:intron |
| GGATGAATTATAACCTCTTG+AGG | 0.577902 | 4.1:-17112926 | None:intergenic |
| AATCTCACTGACAATGGGAA+TGG | 0.580876 | 4.1:+17116495 | MS.gene08361:CDS |
| ATGGGAATGGCTGTTGCACT+TGG | 0.581173 | 4.1:+17116508 | MS.gene08361:CDS |
| CATGTCCAGAATTATCATCA+TGG | 0.585319 | 4.1:-17111415 | None:intergenic |
| ACTCAGGAGGAGCCAACTCG+TGG | 0.586746 | 4.1:+17112001 | MS.gene08361:CDS |
| AGTTCAAGGGAAACCATCAA+AGG | 0.587721 | 4.1:+17110107 | MS.gene08361:intron |
| TGGTATAGGCTCTACTCTGG+GGG | 0.591466 | 4.1:+17116265 | MS.gene08361:intron |
| AGCATTCCTCACCTCAATCA+GGG | 0.600342 | 4.1:-17116307 | None:intergenic |
| CTCAGGAGGAGCCAACTCGT+GGG | 0.602290 | 4.1:+17112002 | MS.gene08361:CDS |
| CTTGCAGAGATGAATAACAA+TGG | 0.603204 | 4.1:+17116439 | MS.gene08361:CDS |
| AAAGTGATGATGGTCGTGAT+GGG | 0.607012 | 4.1:-17109447 | None:intergenic |
| TTCTTGTGGAGAAGCTAGTG+AGG | 0.607811 | 4.1:+17111965 | MS.gene08361:CDS |
| TCAAGTATCACATGACACAT+TGG | 0.608400 | 4.1:+17112158 | MS.gene08361:CDS |
| AGTATCACATGACACATTGG+AGG | 0.613160 | 4.1:+17112161 | MS.gene08361:CDS |
| CATGGTGACCATGCAGACCA+TGG | 0.624284 | 4.1:+17111392 | MS.gene08361:CDS |
| AGGTATGTTGAAGAAAACTC+AGG | 0.625019 | 4.1:+17111985 | MS.gene08361:CDS |
| GTATCACATGACACATTGGA+GGG | 0.631044 | 4.1:+17112162 | MS.gene08361:CDS |
| GGTGAACATTCCCATTCTCA+TGG | 0.633552 | 4.1:+17111374 | MS.gene08361:CDS |
| CATGATGATAATTCTGGACA+TGG | 0.636246 | 4.1:+17111416 | MS.gene08361:CDS |
| AAAAGAGTTTGGTAGATGAG+GGG | 0.647576 | 4.1:+17112124 | MS.gene08361:CDS |
| TAAACCCGATAGTTCAGTAG+AGG | 0.648271 | 4.1:-17109474 | None:intergenic |
| AGTGGAAGCAATGCAACCAA+AGG | 0.649556 | 4.1:+17112597 | MS.gene08361:CDS |
| CAGGAGGAGCCAACTCGTGG+GGG | 0.655963 | 4.1:+17112004 | MS.gene08361:CDS |
| AAGTGATGATGGTCGTGATG+GGG | 0.680703 | 4.1:-17109446 | None:intergenic |
| CTGTGACTGATTGTTGACAT+CGG | 0.696774 | 4.1:-17112085 | None:intergenic |
| TATGTTGAAGAAAACTCAGG+AGG | 0.703074 | 4.1:+17111988 | MS.gene08361:CDS |
| CATATCATACCAAAAGCATG+CGG | 0.734324 | 4.1:-17110453 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17109422 | 17116549 | 17109422 | ID=MS.gene08361 |
| chr4.1 | mRNA | 17109422 | 17116549 | 17109422 | ID=MS.gene08361.t1;Parent=MS.gene08361 |
| chr4.1 | exon | 17109422 | 17109497 | 17109422 | ID=MS.gene08361.t1.exon1;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17109422 | 17109497 | 17109422 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17109915 | 17109998 | 17109915 | ID=MS.gene08361.t1.exon2;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17109915 | 17109998 | 17109915 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17110109 | 17110158 | 17110109 | ID=MS.gene08361.t1.exon3;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17110109 | 17110158 | 17110109 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17110414 | 17110465 | 17110414 | ID=MS.gene08361.t1.exon4;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17110414 | 17110465 | 17110414 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17111369 | 17111485 | 17111369 | ID=MS.gene08361.t1.exon5;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17111369 | 17111485 | 17111369 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17111947 | 17112221 | 17111947 | ID=MS.gene08361.t1.exon6;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17111947 | 17112221 | 17111947 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17112594 | 17112746 | 17112594 | ID=MS.gene08361.t1.exon7;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17112594 | 17112746 | 17112594 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17112827 | 17112934 | 17112827 | ID=MS.gene08361.t1.exon8;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17112827 | 17112934 | 17112827 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17115688 | 17115789 | 17115688 | ID=MS.gene08361.t1.exon9;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17115688 | 17115789 | 17115688 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17116273 | 17116317 | 17116273 | ID=MS.gene08361.t1.exon10;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17116273 | 17116317 | 17116273 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| chr4.1 | exon | 17116394 | 17116549 | 17116394 | ID=MS.gene08361.t1.exon11;Parent=MS.gene08361.t1 |
| chr4.1 | CDS | 17116394 | 17116549 | 17116394 | ID=cds.MS.gene08361.t1;Parent=MS.gene08361.t1 |
| Gene Sequence |
| Protein sequence |
