AlfalfaGEDB | MS.gene08627

M. sativa cultivar XinJiangDaYe / MS.gene08627

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08627.t1	XP_003606392.1	56.7	67	29	0	105	171	100	166	2.50E-08	68.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08627.t1	G7JPD4	56.7	67	29	0	105	171	100	166	1.8e-08	68.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04915	MS.gene08627	0.825495	4.55E-54	-1.69E-46
MS.gene04917	MS.gene08627	0.828882	7.02E-55	-1.69E-46
MS.gene049887	MS.gene08627	0.803451	3.46E-49	-1.69E-46
MS.gene04998	MS.gene08627	0.828178	1.04E-54	-1.69E-46
MS.gene050414	MS.gene08627	0.825507	4.51E-54	-1.69E-46
MS.gene050838	MS.gene08627	0.806621	7.52E-50	-1.69E-46
MS.gene051226	MS.gene08627	0.810437	1.15E-50	-1.69E-46
MS.gene051234	MS.gene08627	0.807263	5.51E-50	-1.69E-46
MS.gene051852	MS.gene08627	0.813224	2.85E-51	-1.69E-46
MS.gene052016	MS.gene08627	0.800211	1.60E-48	-1.69E-46
MS.gene052296	MS.gene08627	0.842861	2.00E-58	-1.69E-46
MS.gene052307	MS.gene08627	0.805726	1.16E-49	-1.69E-46
MS.gene052335	MS.gene08627	0.82122	4.53E-53	-1.69E-46
MS.gene052342	MS.gene08627	0.805565	1.26E-49	-1.69E-46
MS.gene053786	MS.gene08627	0.800566	1.36E-48	-1.69E-46
MS.gene054617	MS.gene08627	0.82704	1.95E-54	-1.69E-46
MS.gene055513	MS.gene08627	0.825214	5.30E-54	-1.69E-46
MS.gene056043	MS.gene08627	-0.807057	6.09E-50	-1.69E-46
MS.gene056285	MS.gene08627	0.812744	3.63E-51	-1.69E-46
MS.gene056752	MS.gene08627	0.816542	5.24E-52	-1.69E-46
MS.gene058720	MS.gene08627	0.826709	2.34E-54	-1.69E-46
MS.gene060932	MS.gene08627	0.829511	4.94E-55	-1.69E-46
MS.gene061100	MS.gene08627	0.833454	5.28E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08627.t1	MTR_4g459420	73.510	151	35	3	24	171	18	166	1.59e-18	78.2
MS.gene08627.t1	MTR_4g059520	73.510	151	35	3	24	171	18	166	1.59e-18	78.2
MS.gene08627.t1	MTR_2g094170	45.000	80	40	2	94	171	71	148	4.21e-18	76.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08627.t1	AT2G28440	40.244	82	45	1	94	171	187	268	2.45e-13	66.2
MS.gene08627.t1	AT3G45230	41.667	180	77	7	11	170	4	175	4.59e-11	58.9

Find 65 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCTATTCCCGCTTTCTTGC+CGG	0.260149	4.1:+39104128	None:intergenic
GGCGGTAGGTGTGGTTGCAT+TGG	0.392536	4.1:-39104091	MS.gene08627:CDS
GAGGGAGTTGGAGTTGGCGC+TGG	0.405081	4.1:+39104321	None:intergenic
TCCGGCTCCGTCCCCGTCGC+CGG	0.421440	4.1:-39104412	MS.gene08627:CDS
GCGGTAGGTGTGGTTGCATT+GGG	0.435389	4.1:-39104090	MS.gene08627:CDS
CGCTTCATCCGCTGATTCTC+CGG	0.443757	4.1:-39104430	MS.gene08627:CDS
AATATTCAGAGATCTGAGTA+TGG	0.445483	4.1:-39104039	MS.gene08627:CDS
TCGGAATGAGGAGAGGGAGT+TGG	0.449101	4.1:+39104309	None:intergenic
GATGGTGAAGGCGAAGAAGA+AGG	0.452099	4.1:+39104249	None:intergenic
GCCACATCCGGCGATGGTAC+TGG	0.452457	4.1:+39104219	None:intergenic
ATGAATTCGTCGCCGGCGAC+GGG	0.458160	4.1:+39104400	None:intergenic
GCCGGCGACGGGGACGGAGC+CGG	0.458942	4.1:+39104411	None:intergenic
GGCGCTGGAGGTGATGTTGC+CGG	0.461320	4.1:+39104336	None:intergenic
GAAGGAGAAGGAGAATTCTC+TGG	0.461384	4.1:+39104267	None:intergenic
CCGTTATCATCCGCCACATC+CGG	0.470921	4.1:+39104207	None:intergenic
TCCAGTACCATCGCCGGATG+TGG	0.483039	4.1:-39104220	MS.gene08627:CDS
TCTGGCGGAGGAATGAGTTC+CGG	0.486197	4.1:-39104147	MS.gene08627:CDS
CACACTGGCATCGGTGGAGA+TGG	0.493285	4.1:-39104177	MS.gene08627:CDS
GGAGGTGATGTTGCCGGAGA+AGG	0.494407	4.1:+39104342	None:intergenic
ACCGATTCCGACTCCTTCTC+CGG	0.497741	4.1:-39104355	MS.gene08627:CDS
GAATTCTCTGGTGAAGGTGC+TGG	0.501742	4.1:+39104279	None:intergenic
CCGGATGTGGCGGATGATAA+CGG	0.506817	4.1:-39104207	MS.gene08627:CDS
GTCGGAATCGGTGTCGGAGA+AGG	0.507353	4.1:+39104366	None:intergenic
GCCGGAGAAGGAGTCGGAAT+CGG	0.509629	4.1:+39104354	None:intergenic
GGTGGAGATGGAAAATCGTC+TGG	0.513722	4.1:-39104165	MS.gene08627:CDS
TTCGTCGCCGGCGACGGGGA+CGG	0.515185	4.1:+39104405	None:intergenic
ATGAGTTCCGGCAAGAAAGC+GGG	0.522666	4.1:-39104135	MS.gene08627:CDS
TGAGGAGAGGGAGTTGGAGT+TGG	0.529383	4.1:+39104315	None:intergenic
GGAGTTGGAGTTGGCGCTGG+AGG	0.535858	4.1:+39104324	None:intergenic
TCCGGCGATGGTACTGGAGA+TGG	0.543032	4.1:+39104225	None:intergenic
GATGAATTCGTCGCCGGCGA+CGG	0.544339	4.1:+39104399	None:intergenic
TGAAGGTGCTGGTGGTGAAT+CGG	0.549645	4.1:+39104290	None:intergenic
GAAGGCGAAGAAGAAGGAGA+AGG	0.551274	4.1:+39104255	None:intergenic
GATGGAAAATCGTCTGGCGG+AGG	0.558778	4.1:-39104159	MS.gene08627:CDS
TTCTCTGGTGAAGGTGCTGG+TGG	0.564561	4.1:+39104282	None:intergenic
GGAGTAAACCACACTGGCAT+CGG	0.567165	4.1:-39104186	MS.gene08627:CDS
GAAGGAGAATTCTCTGGTGA+AGG	0.571771	4.1:+39104273	None:intergenic
GGTGAATCGGAATGAGGAGA+GGG	0.573954	4.1:+39104303	None:intergenic
TGTGGTTGCATTGGGAGCGA+TGG	0.577207	4.1:-39104082	MS.gene08627:CDS
CGATGGTGGTGAAGAAGCGT+AGG	0.581346	4.1:-39104065	MS.gene08627:CDS
AGTACCATCGCCGGATGTGG+CGG	0.585844	4.1:-39104217	MS.gene08627:CDS
TCCATCTCCAGTACCATCGC+CGG	0.589845	4.1:-39104226	MS.gene08627:CDS
GAAGGAGTCGGAATCGGTGT+CGG	0.589970	4.1:+39104360	None:intergenic
AAGAAAGCGGGAATAGCGGT+TGG	0.593108	4.1:-39104123	MS.gene08627:CDS
GGTTGCATTGGGAGCGATGG+TGG	0.601732	4.1:-39104079	MS.gene08627:CDS
TGAATTCGTCGCCGGCGACG+GGG	0.603141	4.1:+39104401	None:intergenic
GTAAACCACACTGGCATCGG+TGG	0.607098	4.1:-39104183	MS.gene08627:CDS
GGACGGAGCCGGAGAATCAG+CGG	0.612625	4.1:+39104422	None:intergenic
TCATCCGCCACATCCGGCGA+TGG	0.614050	4.1:+39104213	None:intergenic
TTTACCGATGAATTCGTCGC+CGG	0.617262	4.1:+39104393	None:intergenic
GTTGGAGTGATAGCGGCGGT+AGG	0.622430	4.1:-39104105	MS.gene08627:CDS
CATCTCCACCGATGCCAGTG+TGG	0.623857	4.1:+39104178	None:intergenic
GATGTTGCCGGAGAAGGAGT+CGG	0.624466	4.1:+39104348	None:intergenic
GTCGCCGGCGACGAATTCAT+CGG	0.624726	4.1:-39104397	MS.gene08627:CDS
CGGCAAGAAAGCGGGAATAG+CGG	0.634612	4.1:-39104127	MS.gene08627:CDS
GCTGGTGGTGAATCGGAATG+AGG	0.641250	4.1:+39104297	None:intergenic
TGGTGAATCGGAATGAGGAG+AGG	0.641453	4.1:+39104302	None:intergenic
AATGAGTTCCGGCAAGAAAG+CGG	0.641771	4.1:-39104136	MS.gene08627:CDS
AGTGATAGCGGCGGTAGGTG+TGG	0.647668	4.1:-39104100	MS.gene08627:CDS
AATAGCGGTTGGAGTGATAG+CGG	0.659529	4.1:-39104112	MS.gene08627:CDS
GATGGTACTGGAGATGGAGA+TGG	0.679255	4.1:+39104231	None:intergenic
ACTGGAGATGGAGATGGTGA+AGG	0.681678	4.1:+39104237	None:intergenic
GGAGATGGAAAATCGTCTGG+CGG	0.696636	4.1:-39104162	MS.gene08627:CDS
GATAACGGAGTAAACCACAC+TGG	0.721701	4.1:-39104192	MS.gene08627:CDS
AGCGGTTGGAGTGATAGCGG+CGG	0.750494	4.1:-39104109	MS.gene08627:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AATATTCAGAGATCTGAGTA+TGG	-	chr4.1:39104476-39104495	MS.gene08627:CDS	30.0%
	AATAGCGGTTGGAGTGATAG+CGG	-	chr4.1:39104403-39104422	MS.gene08627:CDS	45.0%
	AATGAGTTCCGGCAAGAAAG+CGG	-	chr4.1:39104379-39104398	MS.gene08627:CDS	45.0%
	GAAGGAGAAGGAGAATTCTC+TGG	+	chr4.1:39104251-39104270	None:intergenic	45.0%
	GAAGGAGAATTCTCTGGTGA+AGG	+	chr4.1:39104245-39104264	None:intergenic	45.0%
	TTTACCGATGAATTCGTCGC+CGG	+	chr4.1:39104125-39104144	None:intergenic	45.0%
!!	GATAACGGAGTAAACCACAC+TGG	-	chr4.1:39104323-39104342	MS.gene08627:CDS	45.0%
	AAGAAAGCGGGAATAGCGGT+TGG	-	chr4.1:39104392-39104411	MS.gene08627:CDS	50.0%
	ACTGGAGATGGAGATGGTGA+AGG	+	chr4.1:39104281-39104300	None:intergenic	50.0%
	ATGAGTTCCGGCAAGAAAGC+GGG	-	chr4.1:39104380-39104399	MS.gene08627:CDS	50.0%
	GAAGGCGAAGAAGAAGGAGA+AGG	+	chr4.1:39104263-39104282	None:intergenic	50.0%
	GATGGTGAAGGCGAAGAAGA+AGG	+	chr4.1:39104269-39104288	None:intergenic	50.0%
	GGAGATGGAAAATCGTCTGG+CGG	-	chr4.1:39104353-39104372	MS.gene08627:CDS	50.0%
	GGAGTAAACCACACTGGCAT+CGG	-	chr4.1:39104329-39104348	MS.gene08627:CDS	50.0%
	GGTGAATCGGAATGAGGAGA+GGG	+	chr4.1:39104215-39104234	None:intergenic	50.0%
	GGTGGAGATGGAAAATCGTC+TGG	-	chr4.1:39104350-39104369	MS.gene08627:CDS	50.0%
	TGGTGAATCGGAATGAGGAG+AGG	+	chr4.1:39104216-39104235	None:intergenic	50.0%
!	GAATTCTCTGGTGAAGGTGC+TGG	+	chr4.1:39104239-39104258	None:intergenic	50.0%
!	GATGGTACTGGAGATGGAGA+TGG	+	chr4.1:39104287-39104306	None:intergenic	50.0%
!!	TGAAGGTGCTGGTGGTGAAT+CGG	+	chr4.1:39104228-39104247	None:intergenic	50.0%
	CCGTTATCATCCGCCACATC+CGG	+	chr4.1:39104311-39104330	None:intergenic	55.0%
	CGCTATTCCCGCTTTCTTGC+CGG	+	chr4.1:39104390-39104409	None:intergenic	55.0%
	CGGCAAGAAAGCGGGAATAG+CGG	-	chr4.1:39104388-39104407	MS.gene08627:CDS	55.0%
	GAAGGAGTCGGAATCGGTGT+CGG	+	chr4.1:39104158-39104177	None:intergenic	55.0%
	GATGGAAAATCGTCTGGCGG+AGG	-	chr4.1:39104356-39104375	MS.gene08627:CDS	55.0%
	GATGTTGCCGGAGAAGGAGT+CGG	+	chr4.1:39104170-39104189	None:intergenic	55.0%
	GCGGTAGGTGTGGTTGCATT+GGG	-	chr4.1:39104425-39104444	MS.gene08627:CDS	55.0%
	TCCATCTCCAGTACCATCGC+CGG	-	chr4.1:39104289-39104308	MS.gene08627:CDS	55.0%
	TCGGAATGAGGAGAGGGAGT+TGG	+	chr4.1:39104209-39104228	None:intergenic	55.0%
	TCTGGCGGAGGAATGAGTTC+CGG	-	chr4.1:39104368-39104387	MS.gene08627:CDS	55.0%
	TGAGGAGAGGGAGTTGGAGT+TGG	+	chr4.1:39104203-39104222	None:intergenic	55.0%
	TGTGGTTGCATTGGGAGCGA+TGG	-	chr4.1:39104433-39104452	MS.gene08627:CDS	55.0%
!	ACCGATTCCGACTCCTTCTC+CGG	-	chr4.1:39104160-39104179	MS.gene08627:CDS	55.0%
!	CGATGGTGGTGAAGAAGCGT+AGG	-	chr4.1:39104450-39104469	MS.gene08627:CDS	55.0%
!	GCTGGTGGTGAATCGGAATG+AGG	+	chr4.1:39104221-39104240	None:intergenic	55.0%
!	GTAAACCACACTGGCATCGG+TGG	-	chr4.1:39104332-39104351	MS.gene08627:CDS	55.0%
!!	CCGGATGTGGCGGATGATAA+CGG	-	chr4.1:39104308-39104327	MS.gene08627:CDS	55.0%
!!	CGCTTCATCCGCTGATTCTC+CGG	-	chr4.1:39104085-39104104	MS.gene08627:CDS	55.0%
!!	TTCTCTGGTGAAGGTGCTGG+TGG	+	chr4.1:39104236-39104255	None:intergenic	55.0%
	AGTACCATCGCCGGATGTGG+CGG	-	chr4.1:39104298-39104317	MS.gene08627:CDS	60.0%
	ATGAATTCGTCGCCGGCGAC+GGG	+	chr4.1:39104118-39104137	None:intergenic	60.0%
	CATCTCCACCGATGCCAGTG+TGG	+	chr4.1:39104340-39104359	None:intergenic	60.0%
	GATGAATTCGTCGCCGGCGA+CGG	+	chr4.1:39104119-39104138	None:intergenic	60.0%
	GCCGGAGAAGGAGTCGGAAT+CGG	+	chr4.1:39104164-39104183	None:intergenic	60.0%
	GGAGGTGATGTTGCCGGAGA+AGG	+	chr4.1:39104176-39104195	None:intergenic	60.0%
	GGCGGTAGGTGTGGTTGCAT+TGG	-	chr4.1:39104424-39104443	MS.gene08627:CDS	60.0%
	GGTTGCATTGGGAGCGATGG+TGG	-	chr4.1:39104436-39104455	MS.gene08627:CDS	60.0%
	GTCGCCGGCGACGAATTCAT+CGG	-	chr4.1:39104118-39104137	MS.gene08627:CDS	60.0%
	TCCAGTACCATCGCCGGATG+TGG	-	chr4.1:39104295-39104314	MS.gene08627:CDS	60.0%
!	TCCGGCGATGGTACTGGAGA+TGG	+	chr4.1:39104293-39104312	None:intergenic	60.0%
!!	AGCGGTTGGAGTGATAGCGG+CGG	-	chr4.1:39104406-39104425	MS.gene08627:CDS	60.0%
!!	AGTGATAGCGGCGGTAGGTG+TGG	-	chr4.1:39104415-39104434	MS.gene08627:CDS	60.0%
!!	CACACTGGCATCGGTGGAGA+TGG	-	chr4.1:39104338-39104357	MS.gene08627:CDS	60.0%
!!	GTCGGAATCGGTGTCGGAGA+AGG	+	chr4.1:39104152-39104171	None:intergenic	60.0%
!!	GTTGGAGTGATAGCGGCGGT+AGG	-	chr4.1:39104410-39104429	MS.gene08627:CDS	60.0%
	GAGGGAGTTGGAGTTGGCGC+TGG	+	chr4.1:39104197-39104216	None:intergenic	65.0%
	GCCACATCCGGCGATGGTAC+TGG	+	chr4.1:39104299-39104318	None:intergenic	65.0%
	GGACGGAGCCGGAGAATCAG+CGG	+	chr4.1:39104096-39104115	None:intergenic	65.0%
	TCATCCGCCACATCCGGCGA+TGG	+	chr4.1:39104305-39104324	None:intergenic	65.0%
	TGAATTCGTCGCCGGCGACG+GGG	+	chr4.1:39104117-39104136	None:intergenic	65.0%
!	GGAGTTGGAGTTGGCGCTGG+AGG	+	chr4.1:39104194-39104213	None:intergenic	65.0%
!	GGCGCTGGAGGTGATGTTGC+CGG	+	chr4.1:39104182-39104201	None:intergenic	65.0%
	TTCGTCGCCGGCGACGGGGA+CGG	+	chr4.1:39104113-39104132	None:intergenic	75.0%
!	TCCGGCTCCGTCCCCGTCGC+CGG	-	chr4.1:39104103-39104122	MS.gene08627:CDS	80.0%
!	GCCGGCGACGGGGACGGAGC+CGG	+	chr4.1:39104107-39104126	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	39104011	39104526	39104011	ID=MS.gene08627
chr4.1	mRNA	39104011	39104526	39104011	ID=MS.gene08627.t1;Parent=MS.gene08627
chr4.1	exon	39104011	39104526	39104011	ID=MS.gene08627.t1.exon1;Parent=MS.gene08627.t1
chr4.1	CDS	39104011	39104526	39104011	ID=cds.MS.gene08627.t1;Parent=MS.gene08627.t1

Gene Sequence

>MS.gene08627

ATGACGTTGAATTCTTCAGTTGCAATTGCAAGATTCTCTCTTGTTTTTCTTCTTCTTTCTTTCTTAGTCAACATCGCTTCATCCGCTGATTCTCCGGCTCCGTCCCCGTCGCCGGCGACGAATTCATCGGTAAATTCTCCTTCTCCGACACCGATTCCGACTCCTTCTCCGGCAACATCACCTCCAGCGCCAACTCCAACTCCCTCTCCTCATTCCGATTCACCACCAGCACCTTCACCAGAGAATTCTCCTTCTCCTTCTTCTTCGCCTTCACCATCTCCATCTCCAGTACCATCGCCGGATGTGGCGGATGATAACGGAGTAAACCACACTGGCATCGGTGGAGATGGAAAATCGTCTGGCGGAGGAATGAGTTCCGGCAAGAAAGCGGGAATAGCGGTTGGAGTGATAGCGGCGGTAGGTGTGGTTGCATTGGGAGCGATGGTGGTGAAGAAGCGTAGGCAGAATATTCAGAGATCTGAGTATGGATACACAGCAAGAAGAGAACTTCTGTAA

Protein sequence

>MS.gene08627.t1

MTLNSSVAIARFSLVFLLLSFLVNIASSADSPAPSPSPATNSSVNSPSPTPIPTPSPATSPPAPTPTPSPHSDSPPAPSPENSPSPSSSPSPSPSPVPSPDVADDNGVNHTGIGGDGKSSGGGMSSGKKAGIAVGVIAAVGVVALGAMVVKKRRQNIQRSEYGYTARRELL