Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21233.t1 | XP_003591383.1 | 97 | 133 | 4 | 0 | 1 | 133 | 233 | 365 | 1.90E-67 | 265 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21233.t1 | G7ICW3 | 97.0 | 133 | 4 | 0 | 1 | 133 | 233 | 365 | 1.4e-67 | 265.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene21233 | 0.830852 | 2.33E-55 | -1.69E-46 |
| MS.gene049873 | MS.gene21233 | 0.810782 | 9.71E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene21233 | 0.802772 | 4.79E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene21233 | 0.805135 | 1.54E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene21233 | 0.801589 | 8.39E-49 | -1.69E-46 |
| MS.gene051243 | MS.gene21233 | 0.801939 | 7.11E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene21233 | 0.823088 | 1.67E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene21233 | 0.812831 | 3.48E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene21233 | 0.823895 | 1.08E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene21233 | 0.819084 | 1.40E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene21233 | 0.801628 | 8.23E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene21233 | 0.810042 | 1.40E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene21233 | 0.816953 | 4.24E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene21233 | 0.815123 | 1.09E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene21233 | 0.807949 | 3.94E-50 | -1.69E-46 |
| MS.gene053599 | MS.gene21233 | 0.806658 | 7.39E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene21233 | 0.814445 | 1.53E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene21233 | 0.830427 | 2.95E-55 | -1.69E-46 |
| MS.gene055468 | MS.gene21233 | 0.80603 | 1.00E-49 | -1.69E-46 |
| MS.gene055512 | MS.gene21233 | 0.81777 | 2.78E-52 | -1.69E-46 |
| MS.gene055580 | MS.gene21233 | -0.801962 | 7.03E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene21233 | 0.811804 | 5.83E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene21233 | 0.823352 | 1.45E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene21233 | 0.805216 | 1.49E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene21233 | 0.804833 | 1.79E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene21233 | 0.833706 | 4.57E-56 | -1.69E-46 |
| MS.gene057705 | MS.gene21233 | 0.819117 | 1.37E-52 | -1.69E-46 |
| MS.gene058009 | MS.gene21233 | 0.813419 | 2.58E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene21233 | 0.802809 | 4.70E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene21233 | 0.813811 | 2.12E-51 | -1.69E-46 |
| MS.gene059842 | MS.gene21233 | 0.806521 | 7.90E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene21233 | 0.829939 | 3.89E-55 | -1.69E-46 |
| MS.gene060429 | MS.gene21233 | 0.820697 | 5.98E-53 | -1.69E-46 |
| MS.gene060587 | MS.gene21233 | 0.806622 | 7.52E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTAGGCCTTTGAGGACTA+TGG | 0.252101 | 1.4:+69190663 | MS.gene21233:CDS |
| GAGAACCCTTATGCAGAAAA+TGG | 0.296262 | 1.4:+69190613 | MS.gene21233:CDS |
| TGTCTGTGTTTGTGAGTTAT+CGG | 0.344354 | 1.4:+69191542 | MS.gene21233:CDS |
| GCAAGATGTCTGTCGGATTC+TGG | 0.349571 | 1.4:+69191338 | MS.gene21233:CDS |
| ACTACTCTGTTAACTGTGAT+TGG | 0.350378 | 1.4:+69191398 | MS.gene21233:CDS |
| GGTAAGCCATGGCACGGTTC+CGG | 0.376073 | 1.4:+69191373 | MS.gene21233:CDS |
| TTGAATTCTCTAGGCCTTTG+AGG | 0.408768 | 1.4:+69190656 | MS.gene21233:CDS |
| AAGGTTCTCCATTGGAGTAA+AGG | 0.412113 | 1.4:-69191587 | None:intergenic |
| TGGAGTAAAGGCTACACTCT+TGG | 0.432096 | 1.4:-69191575 | None:intergenic |
| GGAGTAAAGGCTACACTCTT+GGG | 0.441900 | 1.4:-69191574 | None:intergenic |
| GACTATGGATCATCTGCAAC+AGG | 0.447825 | 1.4:+69190678 | MS.gene21233:CDS |
| CTACTCTGTTAACTGTGATT+GGG | 0.458444 | 1.4:+69191399 | MS.gene21233:CDS |
| TGCCATGGCTTACCATCTAC+TGG | 0.461844 | 1.4:-69191364 | None:intergenic |
| GGATTCTGGTATCCAGTAGA+TGG | 0.471064 | 1.4:+69191352 | MS.gene21233:CDS |
| GTCTGTGTTTGTGAGTTATC+GGG | 0.479240 | 1.4:+69191543 | MS.gene21233:CDS |
| ACTATTTAAAGGTTCTCCAT+TGG | 0.483409 | 1.4:-69191595 | None:intergenic |
| GAGTGTAGCCTTTACTCCAA+TGG | 0.521817 | 1.4:+69191579 | MS.gene21233:CDS |
| CTGCTCTGAGATATATCAAT+CGG | 0.523134 | 1.4:-69191424 | None:intergenic |
| CAGGATGTGCAATTCACCAT+TGG | 0.528409 | 1.4:+69191307 | MS.gene21233:intron |
| ACTCAGCATCAAGTAGCTCA+TGG | 0.557960 | 1.4:+69191470 | MS.gene21233:CDS |
| GATGTGCAATTCACCATTGG+CGG | 0.581517 | 1.4:+69191310 | MS.gene21233:CDS |
| TTCATTGAGTGGCAAGTCAG+TGG | 0.585865 | 1.4:+69191447 | MS.gene21233:CDS |
| CATCTTGCTTGATCCGCCAA+TGG | 0.594402 | 1.4:-69191323 | None:intergenic |
| ACAGTTAACAGAGTAGTGTC+CGG | 0.602771 | 1.4:-69191392 | None:intergenic |
| GGATCAAGCAAGATGTCTGT+CGG | 0.616205 | 1.4:+69191331 | MS.gene21233:CDS |
| TAGTGTCCGGAACCGTGCCA+TGG | 0.623740 | 1.4:-69191379 | None:intergenic |
| ATCCAGTAGATGGTAAGCCA+TGG | 0.634636 | 1.4:+69191362 | MS.gene21233:CDS |
| AGATGATCCATAGTCCTCAA+AGG | 0.643096 | 1.4:-69190670 | None:intergenic |
| TCTCAGAGCAGTTCATTGAG+TGG | 0.649577 | 1.4:+69191436 | MS.gene21233:CDS |
| GTAGATGGTAAGCCATGGCA+CGG | 0.658775 | 1.4:+69191367 | MS.gene21233:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTAATCATAATGAATTC+TGG | + | chr1.4:69190863-69190882 | MS.gene21233:intron | 15.0% |
| !!! | ATTTTGTATTTCTGATATTA+TGG | + | chr1.4:69190925-69190944 | MS.gene21233:intron | 15.0% |
| !! | AACTCAGTTAATTCAAAATA+GGG | - | chr1.4:69190788-69190807 | None:intergenic | 20.0% |
| !! | ATTATATTTGAGAGAACTTA+TGG | - | chr1.4:69191147-69191166 | None:intergenic | 20.0% |
| !!! | CATATTTTTTTGAATTCTCT+AGG | + | chr1.4:69190647-69190666 | MS.gene21233:CDS | 20.0% |
| ! | GAACTCAGTTAATTCAAAAT+AGG | - | chr1.4:69190789-69190808 | None:intergenic | 25.0% |
| ! | TATGCAGAAAATGGAAAAAA+GGG | + | chr1.4:69190622-69190641 | MS.gene21233:CDS | 25.0% |
| ! | TTATGCAGAAAATGGAAAAA+AGG | + | chr1.4:69190621-69190640 | MS.gene21233:CDS | 25.0% |
| !! | TTTTGAATTAACTGAGTTCT+TGG | + | chr1.4:69190790-69190809 | MS.gene21233:intron | 25.0% |
| !! | TTTTTCCATTTTCTGCATAA+GGG | - | chr1.4:69190621-69190640 | None:intergenic | 25.0% |
| !!! | CGGAGAAATATTTTTTATGA+AGG | - | chr1.4:69191041-69191060 | None:intergenic | 25.0% |
| !!! | TTTTTTCCATTTTCTGCATA+AGG | - | chr1.4:69190622-69190641 | None:intergenic | 25.0% |
| AAATCCTTCTAGATAACTCA+TGG | + | chr1.4:69190819-69190838 | MS.gene21233:intron | 30.0% | |
| AATAGGGATTCAAACATAAG+AGG | - | chr1.4:69190772-69190791 | None:intergenic | 30.0% | |
| CATGCAAATTTATACCTTAC+AGG | + | chr1.4:69191288-69191307 | MS.gene21233:intron | 30.0% | |
| CTCTTCTAAATGCATCATAA+AGG | - | chr1.4:69190718-69190737 | None:intergenic | 30.0% | |
| TGTAGTTTAGTATGTGACTT+GGG | - | chr1.4:69190905-69190924 | None:intergenic | 30.0% | |
| TTGCAATACCTTCTTTACAA+AGG | + | chr1.4:69191008-69191027 | MS.gene21233:intron | 30.0% | |
| TTGGATAAGATATCTTCTAC+CGG | + | chr1.4:69190979-69190998 | MS.gene21233:intron | 30.0% | |
| TTGTAGTTTAGTATGTGACT+TGG | - | chr1.4:69190906-69190925 | None:intergenic | 30.0% | |
| !! | AACAACCAAACTTTAAAGTG+CGG | + | chr1.4:69191078-69191097 | MS.gene21233:intron | 30.0% |
| !! | ATCATAATGAATTCTGGTTC+TGG | + | chr1.4:69190869-69190888 | MS.gene21233:intron | 30.0% |
| !! | GCTATTTTGTCAAACAGTTT+TGG | + | chr1.4:69190954-69190973 | MS.gene21233:intron | 30.0% |
| !!! | AGTTTGGTTGTTTTAGCTAT+TGG | - | chr1.4:69191070-69191089 | None:intergenic | 30.0% |
| !!! | GTTTGGTTGTTTTAGCTATT+GGG | - | chr1.4:69191069-69191088 | None:intergenic | 30.0% |
| !!! | TGCGGTCTTTTTTATTTTTG+TGG | + | chr1.4:69191096-69191115 | MS.gene21233:intron | 30.0% |
| ACTACTCTGTTAACTGTGAT+TGG | + | chr1.4:69191398-69191417 | MS.gene21233:CDS | 35.0% | |
| CTACTCTGTTAACTGTGATT+GGG | + | chr1.4:69191399-69191418 | MS.gene21233:CDS | 35.0% | |
| GCAAGTTTCCTTTGTAAAGA+AGG | - | chr1.4:69191019-69191038 | None:intergenic | 35.0% | |
| GTAGTTTAGTATGTGACTTG+GGG | - | chr1.4:69190904-69190923 | None:intergenic | 35.0% | |
| TAGTCCATGAGTTATCTAGA+AGG | - | chr1.4:69190826-69190845 | None:intergenic | 35.0% | |
| TGTCTGTGTTTGTGAGTTAT+CGG | + | chr1.4:69191542-69191561 | MS.gene21233:CDS | 35.0% | |
| ! | AAACACGCATGATGTCATTT+AGG | + | chr1.4:69191245-69191264 | MS.gene21233:intron | 35.0% |
| ! | TTTTGTGGATGTAGTTGAAG+TGG | + | chr1.4:69191111-69191130 | MS.gene21233:intron | 35.0% |
| !! | AAAGACCGCACTTTAAAGTT+TGG | - | chr1.4:69191086-69191105 | None:intergenic | 35.0% |
| !! | CTGCTCTGAGATATATCAAT+CGG | - | chr1.4:69191427-69191446 | None:intergenic | 35.0% |
| !! | GAAGGTATTGCAAAACAAAC+CGG | - | chr1.4:69191001-69191020 | None:intergenic | 35.0% |
| !!! | TTGTCAAACAGTTTTGGCTT+TGG | + | chr1.4:69190960-69190979 | MS.gene21233:intron | 35.0% |
| AAGGTTCTCCATTGGAGTAA+AGG | - | chr1.4:69191590-69191609 | None:intergenic | 40.0% | |
| ACAGTTAACAGAGTAGTGTC+CGG | - | chr1.4:69191395-69191414 | None:intergenic | 40.0% | |
| AGATGATCCATAGTCCTCAA+AGG | - | chr1.4:69190673-69190692 | None:intergenic | 40.0% | |
| GAGAACCCTTATGCAGAAAA+TGG | + | chr1.4:69190613-69190632 | MS.gene21233:CDS | 40.0% | |
| GTCTGTGTTTGTGAGTTATC+GGG | + | chr1.4:69191543-69191562 | MS.gene21233:CDS | 40.0% | |
| TTGAATTCTCTAGGCCTTTG+AGG | + | chr1.4:69190656-69190675 | MS.gene21233:CDS | 40.0% | |
| !!! | GTTGTTTTAGCTATTGGGCA+TGG | - | chr1.4:69191064-69191083 | None:intergenic | 40.0% |
| ACTCAGCATCAAGTAGCTCA+TGG | + | chr1.4:69191470-69191489 | MS.gene21233:CDS | 45.0% | |
| ATCCAGTAGATGGTAAGCCA+TGG | + | chr1.4:69191362-69191381 | MS.gene21233:CDS | 45.0% | |
| CAGGATGTGCAATTCACCAT+TGG | + | chr1.4:69191307-69191326 | MS.gene21233:intron | 45.0% | |
| GACTATGGATCATCTGCAAC+AGG | + | chr1.4:69190678-69190697 | MS.gene21233:CDS | 45.0% | |
| GAGTGTAGCCTTTACTCCAA+TGG | + | chr1.4:69191579-69191598 | MS.gene21233:CDS | 45.0% | |
| GATGTGCAATTCACCATTGG+CGG | + | chr1.4:69191310-69191329 | MS.gene21233:CDS | 45.0% | |
| GGAGTAAAGGCTACACTCTT+GGG | - | chr1.4:69191577-69191596 | None:intergenic | 45.0% | |
| GGATCAAGCAAGATGTCTGT+CGG | + | chr1.4:69191331-69191350 | MS.gene21233:CDS | 45.0% | |
| GGATTCTGGTATCCAGTAGA+TGG | + | chr1.4:69191352-69191371 | MS.gene21233:CDS | 45.0% | |
| GGTGAATTGCACATCCTGTA+AGG | - | chr1.4:69191305-69191324 | None:intergenic | 45.0% | |
| GTGGATGTAGTTGAAGTGGA+GGG | + | chr1.4:69191115-69191134 | MS.gene21233:intron | 45.0% | |
| TCTCAGAGCAGTTCATTGAG+TGG | + | chr1.4:69191436-69191455 | MS.gene21233:CDS | 45.0% | |
| TGCGTGTTTGCATCATGTGT+TGG | - | chr1.4:69191234-69191253 | None:intergenic | 45.0% | |
| TGGAGTAAAGGCTACACTCT+TGG | - | chr1.4:69191578-69191597 | None:intergenic | 45.0% | |
| TGTGGATGTAGTTGAAGTGG+AGG | + | chr1.4:69191114-69191133 | MS.gene21233:intron | 45.0% | |
| TTCATTGAGTGGCAAGTCAG+TGG | + | chr1.4:69191447-69191466 | MS.gene21233:CDS | 45.0% | |
| !!! | GTTTTAGCTATTGGGCATGG+CGG | - | chr1.4:69191061-69191080 | None:intergenic | 45.0% |
| CATCTTGCTTGATCCGCCAA+TGG | - | chr1.4:69191326-69191345 | None:intergenic | 50.0% | |
| GCAAGATGTCTGTCGGATTC+TGG | + | chr1.4:69191338-69191357 | MS.gene21233:CDS | 50.0% | |
| GTAGATGGTAAGCCATGGCA+CGG | + | chr1.4:69191367-69191386 | MS.gene21233:CDS | 50.0% | |
| TGCCATGGCTTACCATCTAC+TGG | - | chr1.4:69191367-69191386 | None:intergenic | 50.0% | |
| ! | CTCTAGGCCTTTGAGGACTA+TGG | + | chr1.4:69190663-69190682 | MS.gene21233:CDS | 50.0% |
| GGTAAGCCATGGCACGGTTC+CGG | + | chr1.4:69191373-69191392 | MS.gene21233:CDS | 60.0% | |
| TAGTGTCCGGAACCGTGCCA+TGG | - | chr1.4:69191382-69191401 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 69190599 | 69191612 | 69190599 | ID=MS.gene21233 |
| chr1.4 | mRNA | 69190599 | 69191612 | 69190599 | ID=MS.gene21233.t1;Parent=MS.gene21233 |
| chr1.4 | exon | 69190599 | 69190699 | 69190599 | ID=MS.gene21233.t1.exon1;Parent=MS.gene21233.t1 |
| chr1.4 | CDS | 69190599 | 69190699 | 69190599 | ID=cds.MS.gene21233.t1;Parent=MS.gene21233.t1 |
| chr1.4 | exon | 69191310 | 69191612 | 69191310 | ID=MS.gene21233.t1.exon2;Parent=MS.gene21233.t1 |
| chr1.4 | CDS | 69191310 | 69191612 | 69191310 | ID=cds.MS.gene21233.t1;Parent=MS.gene21233.t1 |
| Gene Sequence |
| Protein sequence |
