Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22261.t1 | XP_013459517.1 | 94.2 | 225 | 10 | 2 | 1 | 222 | 1 | 225 | 9.00E-107 | 396.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22261.t1 | A0A072UWV1 | 94.2 | 225 | 10 | 2 | 1 | 222 | 1 | 225 | 6.5e-107 | 396.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene22261 | 0.801928 | 7.14E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene22261 | 0.822407 | 2.41E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene22261 | 0.840897 | 6.59E-58 | -1.69E-46 |
| MS.gene053826 | MS.gene22261 | 0.831498 | 1.61E-55 | -1.69E-46 |
| MS.gene054708 | MS.gene22261 | 0.80072 | 1.26E-48 | -1.69E-46 |
| MS.gene055549 | MS.gene22261 | 0.80766 | 4.53E-50 | -1.69E-46 |
| MS.gene055550 | MS.gene22261 | 0.807742 | 4.36E-50 | -1.69E-46 |
| MS.gene055551 | MS.gene22261 | 0.814731 | 1.33E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene22261 | 0.859981 | 2.84E-63 | -1.69E-46 |
| MS.gene056311 | MS.gene22261 | 0.843154 | 1.67E-58 | -1.69E-46 |
| MS.gene056313 | MS.gene22261 | 0.837841 | 4.10E-57 | -1.69E-46 |
| MS.gene056408 | MS.gene22261 | 0.842389 | 2.66E-58 | -1.69E-46 |
| MS.gene057014 | MS.gene22261 | 0.818984 | 1.47E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene22261 | 0.813902 | 2.02E-51 | -1.69E-46 |
| MS.gene058173 | MS.gene22261 | 0.802645 | 5.08E-49 | -1.69E-46 |
| MS.gene058175 | MS.gene22261 | 0.810049 | 1.40E-50 | -1.69E-46 |
| MS.gene058493 | MS.gene22261 | 0.800929 | 1.14E-48 | -1.69E-46 |
| MS.gene058494 | MS.gene22261 | 0.813115 | 3.01E-51 | -1.69E-46 |
| MS.gene058628 | MS.gene22261 | 0.85293 | 3.34E-61 | -1.69E-46 |
| MS.gene058629 | MS.gene22261 | 0.838487 | 2.79E-57 | -1.69E-46 |
| MS.gene058630 | MS.gene22261 | 0.854792 | 9.72E-62 | -1.69E-46 |
| MS.gene059690 | MS.gene22261 | 0.801116 | 1.05E-48 | -1.69E-46 |
| MS.gene059694 | MS.gene22261 | 0.803513 | 3.36E-49 | -1.69E-46 |
| MS.gene059697 | MS.gene22261 | 0.803887 | 2.81E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22261.t1 | MTR_3g045790 | 96.444 | 225 | 5 | 2 | 1 | 222 | 1 | 225 | 5.64e-158 | 436 |
| MS.gene22261.t1 | MTR_5g089820 | 60.455 | 220 | 70 | 4 | 1 | 217 | 1 | 206 | 8.60e-86 | 253 |
| MS.gene22261.t1 | MTR_4g118680 | 78.571 | 42 | 9 | 0 | 164 | 205 | 4 | 45 | 2.93e-15 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22261.t1 | AT5G12340 | 44.385 | 187 | 94 | 5 | 33 | 209 | 22 | 208 | 1.16e-45 | 151 |
| MS.gene22261.t1 | AT5G12340 | 44.385 | 187 | 94 | 5 | 33 | 209 | 25 | 211 | 1.17e-45 | 151 |
| MS.gene22261.t1 | AT1G28190 | 26.733 | 202 | 129 | 5 | 9 | 202 | 25 | 215 | 1.53e-12 | 65.5 |
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAAGCTTGAAACTATTT+TGG | 0.091162 | 3.4:-38469367 | MS.gene22261:CDS |
| CGTCAGCTAAGCTTATATTT+TGG | 0.226292 | 3.4:-38469857 | MS.gene22261:CDS |
| AACAAGTCAACAATCAAATT+TGG | 0.260285 | 3.4:-38469591 | MS.gene22261:CDS |
| TTGCATGAAAAGAGATCAAT+AGG | 0.299018 | 3.4:+38469669 | None:intergenic |
| CGGACACCCGATCCCTAGTT+TGG | 0.310287 | 3.4:-38469736 | MS.gene22261:CDS |
| TTCATCACAAGGCTTGTTAA+TGG | 0.314435 | 3.4:-38469504 | MS.gene22261:CDS |
| GCGTGACAAACCATCGTCTC+TGG | 0.356966 | 3.4:+38469774 | None:intergenic |
| TGAAATGCTTGTGAAGTCTA+AGG | 0.382530 | 3.4:-38469403 | MS.gene22261:CDS |
| CTTGTTAATGGAGATAGATC+AGG | 0.402456 | 3.4:-38469492 | MS.gene22261:CDS |
| CAATCCTTCAAATACTCAAA+AGG | 0.411864 | 3.4:+38469561 | None:intergenic |
| TCCAAAATAGTTTCAAGCTT+AGG | 0.413585 | 3.4:+38469366 | None:intergenic |
| TTAGAAGACGAACTCTTTCA+AGG | 0.441583 | 3.4:-38469711 | MS.gene22261:CDS |
| GCTTGTGAAGTCTAAGGATC+AGG | 0.441885 | 3.4:-38469397 | MS.gene22261:CDS |
| TATCTAGCAGACACAGTTTG+TGG | 0.463540 | 3.4:+38469297 | None:intergenic |
| TCGTCTTCTAAATCCAAACT+AGG | 0.463904 | 3.4:+38469723 | None:intergenic |
| TGATGGAAAAGTGTTGATAA+AGG | 0.464603 | 3.4:-38469538 | MS.gene22261:CDS |
| AAACAAGGTTAGACTTAGAA+AGG | 0.491346 | 3.4:-38469343 | MS.gene22261:CDS |
| CTAAATCCAAACTAGGGATC+GGG | 0.491708 | 3.4:+38469730 | None:intergenic |
| AATCCTTCAAATACTCAAAA+GGG | 0.496327 | 3.4:+38469562 | None:intergenic |
| TGAAGTCTAAGGATCAGGTT+TGG | 0.505035 | 3.4:-38469392 | MS.gene22261:CDS |
| TCTAAATCCAAACTAGGGAT+CGG | 0.527145 | 3.4:+38469729 | None:intergenic |
| ACAAGCCTTGTGATGAACTC+TGG | 0.550575 | 3.4:+38469510 | None:intergenic |
| CGTGACAAACCATCGTCTCT+GGG | 0.573900 | 3.4:+38469775 | None:intergenic |
| CTAAACGGTAAGCACATCGC+TGG | 0.579320 | 3.4:-38469816 | MS.gene22261:CDS |
| GGTTCCACGAGATTGCTAAA+CGG | 0.584296 | 3.4:-38469831 | MS.gene22261:CDS |
| CGTCTTCTAAATCCAAACTA+GGG | 0.587535 | 3.4:+38469724 | None:intergenic |
| TTGTTAATGGAGATAGATCA+GGG | 0.611228 | 3.4:-38469491 | MS.gene22261:CDS |
| TCAACTCAGGGGTACTACAA+AGG | 0.612009 | 3.4:+38469436 | None:intergenic |
| GAGTATTTGAAGGATTGTGA+TGG | 0.613286 | 3.4:-38469555 | MS.gene22261:CDS |
| TGCATGAAAAGAGATCAATA+GGG | 0.622739 | 3.4:+38469670 | None:intergenic |
| AATATAAGCTTAGCTGACGA+TGG | 0.633636 | 3.4:+38469861 | None:intergenic |
| CTTACCGTTTAGCAATCTCG+TGG | 0.635041 | 3.4:+38469827 | None:intergenic |
| GTTCGAGTACCCAGAGACGA+TGG | 0.655665 | 3.4:-38469784 | MS.gene22261:CDS |
| TTATGCCAGAGTTCATCACA+AGG | 0.712497 | 3.4:-38469515 | MS.gene22261:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAGTCAACAATCAAATT+TGG | - | chr3.4:38469647-38469666 | MS.gene22261:CDS | 25.0% |
| ! | AATCCTTCAAATACTCAAAA+GGG | + | chr3.4:38469679-38469698 | None:intergenic | 25.0% |
| !! | GATGATAATTTTCTGTTTTG+AGG | + | chr3.4:38469332-38469351 | None:intergenic | 25.0% |
| !!! | ATAATGTTTTTTCAACTCAG+GGG | + | chr3.4:38469816-38469835 | None:intergenic | 25.0% |
| !!! | CATAATGTTTTTTCAACTCA+GGG | + | chr3.4:38469817-38469836 | None:intergenic | 25.0% |
| !!! | TCATAATGTTTTTTCAACTC+AGG | + | chr3.4:38469818-38469837 | None:intergenic | 25.0% |
| !!! | TGAAACTATTTTGGAAAACA+AGG | - | chr3.4:38469880-38469899 | MS.gene22261:CDS | 25.0% |
| AAACAAGGTTAGACTTAGAA+AGG | - | chr3.4:38469895-38469914 | MS.gene22261:CDS | 30.0% | |
| AAAGGAAAGAAAAAGAGAGA+AGG | - | chr3.4:38469913-38469932 | MS.gene22261:CDS | 30.0% | |
| CAATCCTTCAAATACTCAAA+AGG | + | chr3.4:38469680-38469699 | None:intergenic | 30.0% | |
| TCCAAAATAGTTTCAAGCTT+AGG | + | chr3.4:38469875-38469894 | None:intergenic | 30.0% | |
| TGCATGAAAAGAGATCAATA+GGG | + | chr3.4:38469571-38469590 | None:intergenic | 30.0% | |
| TTGCATGAAAAGAGATCAAT+AGG | + | chr3.4:38469572-38469591 | None:intergenic | 30.0% | |
| TTGTTAATGGAGATAGATCA+GGG | - | chr3.4:38469747-38469766 | MS.gene22261:CDS | 30.0% | |
| ! | TCCTAAGCTTGAAACTATTT+TGG | - | chr3.4:38469871-38469890 | MS.gene22261:CDS | 30.0% |
| !! | TGATGGAAAAGTGTTGATAA+AGG | - | chr3.4:38469700-38469719 | MS.gene22261:CDS | 30.0% |
| AATATAAGCTTAGCTGACGA+TGG | + | chr3.4:38469380-38469399 | None:intergenic | 35.0% | |
| CGTCTTCTAAATCCAAACTA+GGG | + | chr3.4:38469517-38469536 | None:intergenic | 35.0% | |
| CTTGTTAATGGAGATAGATC+AGG | - | chr3.4:38469746-38469765 | MS.gene22261:CDS | 35.0% | |
| GAGTATTTGAAGGATTGTGA+TGG | - | chr3.4:38469683-38469702 | MS.gene22261:CDS | 35.0% | |
| TCGTCTTCTAAATCCAAACT+AGG | + | chr3.4:38469518-38469537 | None:intergenic | 35.0% | |
| TCTAAATCCAAACTAGGGAT+CGG | + | chr3.4:38469512-38469531 | None:intergenic | 35.0% | |
| TGAAATGCTTGTGAAGTCTA+AGG | - | chr3.4:38469835-38469854 | MS.gene22261:CDS | 35.0% | |
| TTAGAAGACGAACTCTTTCA+AGG | - | chr3.4:38469527-38469546 | MS.gene22261:CDS | 35.0% | |
| ! | CGTCAGCTAAGCTTATATTT+TGG | - | chr3.4:38469381-38469400 | MS.gene22261:CDS | 35.0% |
| !! | TTCATCACAAGGCTTGTTAA+TGG | - | chr3.4:38469734-38469753 | MS.gene22261:CDS | 35.0% |
| !!! | GCTAAGCTTATATTTTGGCA+AGG | - | chr3.4:38469386-38469405 | MS.gene22261:CDS | 35.0% |
| CTAAATCCAAACTAGGGATC+GGG | + | chr3.4:38469511-38469530 | None:intergenic | 40.0% | |
| TATCTAGCAGACACAGTTTG+TGG | + | chr3.4:38469944-38469963 | None:intergenic | 40.0% | |
| TGAAGTCTAAGGATCAGGTT+TGG | - | chr3.4:38469846-38469865 | MS.gene22261:CDS | 40.0% | |
| TTATGCCAGAGTTCATCACA+AGG | - | chr3.4:38469723-38469742 | MS.gene22261:CDS | 40.0% | |
| !! | GTGCCCTTTTGAGTATTTGA+AGG | - | chr3.4:38469673-38469692 | MS.gene22261:CDS | 40.0% |
| !!! | CTGTTTTGAGGATGAAAGCA+TGG | + | chr3.4:38469320-38469339 | None:intergenic | 40.0% |
| CTTACCGTTTAGCAATCTCG+TGG | + | chr3.4:38469414-38469433 | None:intergenic | 45.0% | |
| GCTTGTGAAGTCTAAGGATC+AGG | - | chr3.4:38469841-38469860 | MS.gene22261:CDS | 45.0% | |
| GGTTCCACGAGATTGCTAAA+CGG | - | chr3.4:38469407-38469426 | MS.gene22261:CDS | 45.0% | |
| ! | ACAAGCCTTGTGATGAACTC+TGG | + | chr3.4:38469731-38469750 | None:intergenic | 45.0% |
| !! | TCAACTCAGGGGTACTACAA+AGG | + | chr3.4:38469805-38469824 | None:intergenic | 45.0% |
| !!! | CACGCTGACTCGTTTTTCAT+CGG | - | chr3.4:38469482-38469501 | MS.gene22261:CDS | 45.0% |
| CGTGACAAACCATCGTCTCT+GGG | + | chr3.4:38469466-38469485 | None:intergenic | 50.0% | |
| CTAAACGGTAAGCACATCGC+TGG | - | chr3.4:38469422-38469441 | MS.gene22261:CDS | 50.0% | |
| GCGTGACAAACCATCGTCTC+TGG | + | chr3.4:38469467-38469486 | None:intergenic | 55.0% | |
| GTTCGAGTACCCAGAGACGA+TGG | - | chr3.4:38469454-38469473 | MS.gene22261:CDS | 55.0% | |
| CGGACACCCGATCCCTAGTT+TGG | - | chr3.4:38469502-38469521 | MS.gene22261:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 38469296 | 38469964 | 38469296 | ID=MS.gene22261 |
| chr3.4 | mRNA | 38469296 | 38469964 | 38469296 | ID=MS.gene22261.t1;Parent=MS.gene22261 |
| chr3.4 | exon | 38469296 | 38469964 | 38469296 | ID=MS.gene22261.t1.exon1;Parent=MS.gene22261.t1 |
| chr3.4 | CDS | 38469296 | 38469964 | 38469296 | ID=cds.MS.gene22261.t1;Parent=MS.gene22261.t1 |
| Gene Sequence |
| Protein sequence |
