Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23250.t1 | XP_003622774.1 | 100 | 45 | 0 | 0 | 8 | 52 | 1 | 45 | 4.70E-14 | 86.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23250.t1 | G7KY15 | 100.0 | 45 | 0 | 0 | 8 | 52 | 1 | 45 | 3.4e-14 | 86.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene23250 | 0.866022 | 3.86E-65 | -1.69E-46 |
| MS.gene049915 | MS.gene23250 | 0.805692 | 1.18E-49 | -1.69E-46 |
| MS.gene050099 | MS.gene23250 | 0.822876 | 1.87E-53 | -1.69E-46 |
| MS.gene050301 | MS.gene23250 | 0.830374 | 3.04E-55 | -1.69E-46 |
| MS.gene051471 | MS.gene23250 | 0.814623 | 1.40E-51 | -1.69E-46 |
| MS.gene051662 | MS.gene23250 | 0.831762 | 1.39E-55 | -1.69E-46 |
| MS.gene051663 | MS.gene23250 | 0.841407 | 4.84E-58 | -1.69E-46 |
| MS.gene052078 | MS.gene23250 | 0.815807 | 7.65E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene23250 | 0.860302 | 2.27E-63 | -1.69E-46 |
| MS.gene054184 | MS.gene23250 | 0.817879 | 2.62E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene23250 | 0.825469 | 4.61E-54 | -1.69E-46 |
| MS.gene054667 | MS.gene23250 | 0.816845 | 4.48E-52 | -1.69E-46 |
| MS.gene055133 | MS.gene23250 | 0.831152 | 1.96E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene23250 | 0.838758 | 2.38E-57 | -1.69E-46 |
| MS.gene055390 | MS.gene23250 | 0.830993 | 2.15E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene23250 | 0.860187 | 2.46E-63 | -1.69E-46 |
| MS.gene055428 | MS.gene23250 | 0.817997 | 2.47E-52 | -1.69E-46 |
| MS.gene057121 | MS.gene23250 | 0.80955 | 1.79E-50 | -1.69E-46 |
| MS.gene057260 | MS.gene23250 | 0.82328 | 1.51E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene23250 | 0.826672 | 2.39E-54 | -1.69E-46 |
| MS.gene057754 | MS.gene23250 | 0.813839 | 2.09E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene23250 | 0.832414 | 9.58E-56 | -1.69E-46 |
| MS.gene05811 | MS.gene23250 | 0.840849 | 6.79E-58 | -1.69E-46 |
| MS.gene058660 | MS.gene23250 | 0.816472 | 5.43E-52 | -1.69E-46 |
| MS.gene058845 | MS.gene23250 | 0.807841 | 4.15E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene23250 | 0.820282 | 7.45E-53 | -1.69E-46 |
| MS.gene059518 | MS.gene23250 | 0.823203 | 1.57E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene23250 | 0.821358 | 4.21E-53 | -1.69E-46 |
| MS.gene059894 | MS.gene23250 | 0.803565 | 3.28E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene23250 | 0.851501 | 8.52E-61 | -1.69E-46 |
| MS.gene060476 | MS.gene23250 | 0.869764 | 2.42E-66 | -1.69E-46 |
| MS.gene061049 | MS.gene23250 | 0.830108 | 3.54E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 9 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAGTAGCATGAGTACTTT+TGG | 0.271602 | 7.3:+52161436 | None:intergenic |
| AACTTTAGCTGCAAGAATTA+AGG | 0.311122 | 7.3:+52161411 | None:intergenic |
| TTCAAACTTAGTTCTCTTTG+AGG | 0.370756 | 7.3:+52160779 | None:intergenic |
| TTTGCAGGAATTGAAACAAA+AGG | 0.418042 | 7.3:-52160824 | MS.gene23250:intron |
| TCAAAGAGAACTAAGTTTGA+AGG | 0.442646 | 7.3:-52160777 | None:intergenic |
| CTTGCAGCTAAAGTTATACA+AGG | 0.476292 | 7.3:-52161403 | MS.gene23250:intron |
| TTGCAGGAATTGAAACAAAA+GGG | 0.500483 | 7.3:-52160823 | MS.gene23250:intron |
| TAGTTCTCTTTGAGGTTGTG+AGG | 0.552834 | 7.3:+52160787 | None:intergenic |
| CAGGAATTGAAACAAAAGGG+TGG | 0.635486 | 7.3:-52160820 | MS.gene23250:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATTCTTCTTATTATTATAT+TGG | - | chr7.3:52160993-52161012 | MS.gene23250:intron | 15.0% |
| !!! | ATGTAATTTTGTAAAACTTA+TGG | + | chr7.3:52161191-52161210 | None:intergenic | 15.0% |
| !!! | TAAGTTTTACAAAATTACAT+TGG | - | chr7.3:52161191-52161210 | MS.gene23250:intron | 15.0% |
| !! | AAAAATACACCAATAAAGTA+AGG | + | chr7.3:52161117-52161136 | None:intergenic | 20.0% |
| !! | CATATTATACCTTACTTTAT+TGG | - | chr7.3:52161105-52161124 | MS.gene23250:intron | 20.0% |
| !! | TCTTTCAATTGTATATAGTT+TGG | - | chr7.3:52161021-52161040 | MS.gene23250:intron | 20.0% |
| ! | AAATTAGTGCATGCTTAAAT+TGG | + | chr7.3:52161290-52161309 | None:intergenic | 25.0% |
| ! | AAATTTGTAACATTCTTTGC+AGG | - | chr7.3:52161415-52161434 | MS.gene23250:CDS | 25.0% |
| ! | AACAAAAAAAAAGAGGAGAA+TGG | - | chr7.3:52160781-52160800 | MS.gene23250:CDS | 25.0% |
| ! | ACAAAAAAAAAGAGGAGAAT+GGG | - | chr7.3:52160782-52160801 | MS.gene23250:CDS | 25.0% |
| ! | AGAATTCATTGGAACTTTAT+AGG | + | chr7.3:52160926-52160945 | None:intergenic | 25.0% |
| ! | GAATCATAGTATCATTAAGT+AGG | + | chr7.3:52160896-52160915 | None:intergenic | 25.0% |
| ! | GCAAAGAATGTTACAAATTT+AGG | + | chr7.3:52161415-52161434 | None:intergenic | 25.0% |
| ! | TTAAAACTTCAACAAAATCG+TGG | + | chr7.3:52161219-52161238 | None:intergenic | 25.0% |
| ! | TTGTAAAACTTATGGCAATT+TGG | + | chr7.3:52161183-52161202 | None:intergenic | 25.0% |
| !! | TGAAAAAAAGAAAGAGTGTA+AGG | + | chr7.3:52160964-52160983 | None:intergenic | 25.0% |
| !!! | TTTCTTTTTTTCACTTCTCT+TGG | - | chr7.3:52160971-52160990 | MS.gene23250:intron | 25.0% |
| AACTTTAGCTGCAAGAATTA+AGG | + | chr7.3:52160846-52160865 | None:intergenic | 30.0% | |
| AGCACTTCTCTAAATTATCA+AGG | - | chr7.3:52161317-52161336 | MS.gene23250:intron | 30.0% | |
| TTGCAGGAATTGAAACAAAA+GGG | - | chr7.3:52161431-52161450 | MS.gene23250:CDS | 30.0% | |
| TTTGCAGGAATTGAAACAAA+AGG | - | chr7.3:52161430-52161449 | MS.gene23250:CDS | 30.0% | |
| ! | TTCAAACTTAGTTCTCTTTG+AGG | + | chr7.3:52161478-52161497 | None:intergenic | 30.0% |
| AGAAGAAGGTGAGAATTCAT+TGG | + | chr7.3:52160937-52160956 | None:intergenic | 35.0% | |
| CTTGCAGCTAAAGTTATACA+AGG | - | chr7.3:52160851-52160870 | MS.gene23250:intron | 35.0% | |
| TTAGTGCATGCTTAAATTGG+TGG | + | chr7.3:52161287-52161306 | None:intergenic | 35.0% | |
| ! | AAGAGTAGCATGAGTACTTT+TGG | + | chr7.3:52160821-52160840 | None:intergenic | 35.0% |
| ! | AGAGTAGCATGAGTACTTTT+GGG | + | chr7.3:52160820-52160839 | None:intergenic | 35.0% |
| CAGGAATTGAAACAAAAGGG+TGG | - | chr7.3:52161434-52161453 | MS.gene23250:CDS | 40.0% | |
| GAGTGTAAGGACTAAGAAGA+AGG | + | chr7.3:52160951-52160970 | None:intergenic | 40.0% | |
| ! | TGCATGCTTAAATTGGTGGT+GGG | + | chr7.3:52161283-52161302 | None:intergenic | 40.0% |
| !! | CATGAGTACTTTTGGGAGTT+TGG | + | chr7.3:52160813-52160832 | None:intergenic | 40.0% |
| !! | TAGTTCTCTTTGAGGTTGTG+AGG | + | chr7.3:52161470-52161489 | None:intergenic | 40.0% |
| GTGCATGCTTAAATTGGTGG+TGG | + | chr7.3:52161284-52161303 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 52160778 | 52161498 | 52160778 | ID=MS.gene23250 |
| chr7.3 | mRNA | 52160778 | 52161498 | 52160778 | ID=MS.gene23250.t1;Parent=MS.gene23250 |
| chr7.3 | exon | 52161404 | 52161498 | 52161404 | ID=MS.gene23250.t1.exon1;Parent=MS.gene23250.t1 |
| chr7.3 | CDS | 52161404 | 52161498 | 52161404 | ID=cds.MS.gene23250.t1;Parent=MS.gene23250.t1 |
| chr7.3 | exon | 52160778 | 52160839 | 52160778 | ID=MS.gene23250.t1.exon2;Parent=MS.gene23250.t1 |
| chr7.3 | CDS | 52160778 | 52160839 | 52160778 | ID=cds.MS.gene23250.t1;Parent=MS.gene23250.t1 |
| Gene Sequence |
| Protein sequence |
