Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25468.t1 | XP_004511215.1 | 98.1 | 53 | 1 | 0 | 1 | 53 | 1 | 53 | 1.10E-21 | 111.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25468.t1 | Q9C9L0 | 92.5 | 53 | 4 | 0 | 1 | 53 | 1 | 53 | 4.2e-22 | 104.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25468.t1 | A0A1S2YWU8 | 98.1 | 53 | 1 | 0 | 1 | 53 | 1 | 53 | 7.9e-22 | 111.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene25468 | 0.816552 | 5.21E-52 | -1.69E-46 |
| MS.gene049368 | MS.gene25468 | 0.809864 | 1.53E-50 | -1.69E-46 |
| MS.gene049585 | MS.gene25468 | 0.802298 | 6.00E-49 | -1.69E-46 |
| MS.gene050361 | MS.gene25468 | 0.802892 | 4.52E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene25468 | 0.83055 | 2.76E-55 | -1.69E-46 |
| MS.gene050941 | MS.gene25468 | 0.816976 | 4.19E-52 | -1.69E-46 |
| MS.gene051102 | MS.gene25468 | 0.81536 | 9.62E-52 | -1.69E-46 |
| MS.gene051800 | MS.gene25468 | 0.82305 | 1.71E-53 | -1.69E-46 |
| MS.gene052307 | MS.gene25468 | 0.832505 | 9.10E-56 | -1.69E-46 |
| MS.gene052609 | MS.gene25468 | 0.853408 | 2.44E-61 | -1.69E-46 |
| MS.gene05350 | MS.gene25468 | 0.818257 | 2.15E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene25468 | 0.802949 | 4.40E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene25468 | 0.805857 | 1.09E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene25468 | 0.809221 | 2.11E-50 | -1.69E-46 |
| MS.gene055311 | MS.gene25468 | 0.812502 | 4.10E-51 | -1.69E-46 |
| MS.gene055313 | MS.gene25468 | 0.818563 | 1.84E-52 | -1.69E-46 |
| MS.gene055513 | MS.gene25468 | 0.802805 | 4.71E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene25468 | 0.854016 | 1.63E-61 | -1.69E-46 |
| MS.gene055926 | MS.gene25468 | 0.813451 | 2.54E-51 | -1.69E-46 |
| MS.gene056045 | MS.gene25468 | 0.801647 | 8.16E-49 | -1.69E-46 |
| MS.gene056480 | MS.gene25468 | 0.800769 | 1.23E-48 | -1.69E-46 |
| MS.gene056507 | MS.gene25468 | 0.80683 | 6.80E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene25468 | 0.816975 | 4.19E-52 | -1.69E-46 |
| MS.gene058081 | MS.gene25468 | 0.84024 | 9.79E-58 | -1.69E-46 |
| MS.gene058289 | MS.gene25468 | 0.830049 | 3.66E-55 | -1.69E-46 |
| MS.gene058292 | MS.gene25468 | 0.8195 | 1.12E-52 | -1.69E-46 |
| MS.gene058429 | MS.gene25468 | 0.819674 | 1.03E-52 | -1.69E-46 |
| MS.gene058638 | MS.gene25468 | 0.803989 | 2.68E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene25468 | 0.8036 | 3.23E-49 | -1.69E-46 |
| MS.gene058647 | MS.gene25468 | 0.806689 | 7.28E-50 | -1.69E-46 |
| MS.gene058758 | MS.gene25468 | 0.814386 | 1.58E-51 | -1.69E-46 |
| MS.gene059439 | MS.gene25468 | 0.842362 | 2.71E-58 | -1.69E-46 |
| MS.gene06010 | MS.gene25468 | 0.810751 | 9.86E-51 | -1.69E-46 |
| MS.gene060380 | MS.gene25468 | 0.85473 | 1.01E-61 | -1.69E-46 |
| MS.gene06052 | MS.gene25468 | 0.835262 | 1.86E-56 | -1.69E-46 |
| MS.gene060777 | MS.gene25468 | 0.811359 | 7.28E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene25468 | 0.820095 | 8.22E-53 | -1.69E-46 |
| MS.gene060933 | MS.gene25468 | 0.806457 | 8.15E-50 | -1.69E-46 |
| MS.gene06121 | MS.gene25468 | 0.811829 | 5.76E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25468.t1 | MTR_8g056030 | 94.340 | 53 | 2 | 1 | 1 | 53 | 1 | 52 | 1.27e-28 | 105 |
| MS.gene25468.t1 | MTR_5g039880 | 79.245 | 53 | 11 | 0 | 1 | 53 | 1 | 53 | 1.23e-25 | 97.1 |
| MS.gene25468.t1 | MTR_7g039690 | 79.245 | 53 | 11 | 0 | 1 | 53 | 1 | 53 | 1.28e-25 | 97.1 |
| MS.gene25468.t1 | MTR_6g011700 | 77.551 | 49 | 11 | 0 | 5 | 53 | 2 | 50 | 1.06e-20 | 83.2 |
| MS.gene25468.t1 | MTR_7g039680 | 72.917 | 48 | 13 | 0 | 6 | 53 | 3 | 50 | 7.61e-20 | 80.1 |
| MS.gene25468.t1 | MTR_5g039890 | 72.917 | 48 | 13 | 0 | 6 | 53 | 3 | 50 | 4.04e-19 | 78.6 |
| MS.gene25468.t1 | MTR_5g036450 | 63.636 | 55 | 18 | 1 | 1 | 55 | 1 | 53 | 6.09e-19 | 72.8 |
| MS.gene25468.t1 | MTR_7g039660 | 66.038 | 53 | 17 | 1 | 1 | 53 | 1 | 52 | 1.29e-17 | 74.3 |
| MS.gene25468.t1 | MTR_6g011770 | 68.750 | 48 | 15 | 0 | 5 | 52 | 2 | 49 | 2.15e-17 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25468.t1 | AT1G69670 | 92.453 | 53 | 4 | 0 | 1 | 53 | 1 | 53 | 4.98e-29 | 107 |
| MS.gene25468.t1 | AT1G69670 | 92.453 | 53 | 4 | 0 | 1 | 53 | 1 | 53 | 4.98e-29 | 107 |
| MS.gene25468.t1 | AT1G26830 | 86.792 | 53 | 7 | 0 | 1 | 53 | 1 | 53 | 1.15e-27 | 103 |
Find 8 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGAAAATTCTTCTTCTTT+TGG | 0.243349 | 8.4:-42962845 | None:intergenic |
| TTAGCTTTGAAGAACTTTAC+AGG | 0.291791 | 8.4:+42962976 | MS.gene25468:CDS |
| CATGTCTTATCTGCATATTT+CGG | 0.358065 | 8.4:-42962900 | None:intergenic |
| GTTTAAGCATAGGGTTGTTA+TGG | 0.383121 | 8.4:+42962875 | MS.gene25468:CDS |
| GATTGAAGCGTTTAAGCATA+GGG | 0.453200 | 8.4:+42962866 | MS.gene25468:CDS |
| AGATTGAAGCGTTTAAGCAT+AGG | 0.520162 | 8.4:+42962865 | MS.gene25468:CDS |
| CGAAATATGCAGATAAGACA+TGG | 0.541743 | 8.4:+42962901 | MS.gene25468:CDS |
| ATTTATAATCATAACGCTAG+TGG | 0.606333 | 8.4:+42962951 | MS.gene25468:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGTGTAATTTTAATTTTTT+TGG | + | chr8.4:42963100-42963119 | MS.gene25468:intron | 10.0% |
| !!! | TGTGTAATTTTAATTTTTTT+GGG | + | chr8.4:42963101-42963120 | MS.gene25468:intron | 10.0% |
| !! | TACAATAAATCAAACAAAAA+TGG | - | chr8.4:42963076-42963095 | None:intergenic | 15.0% |
| !!! | TTTTGTTTGATTTATTGTAT+TGG | + | chr8.4:42963077-42963096 | MS.gene25468:intron | 15.0% |
| !!! | CATGATTATGATTTATTTCT+TGG | + | chr8.4:42963043-42963062 | MS.gene25468:intron | 20.0% |
| ! | ATTTATAATCATAACGCTAG+TGG | + | chr8.4:42962951-42962970 | MS.gene25468:CDS | 25.0% |
| !! | AAGTAATTTGTAGCATTTCT+TGG | + | chr8.4:42963186-42963205 | MS.gene25468:intron | 25.0% |
| !! | AGTAATTTGTAGCATTTCTT+GGG | + | chr8.4:42963187-42963206 | MS.gene25468:intron | 25.0% |
| !! | TCTGAAAATTCTTCTTCTTT+TGG | - | chr8.4:42962848-42962867 | None:intergenic | 25.0% |
| !!! | GTTATAGGATTTTAGGTTTT+TGG | + | chr8.4:42963142-42963161 | MS.gene25468:intron | 25.0% |
| !!! | TTTTTTTGGGTAAGATTTAG+AGG | + | chr8.4:42963114-42963133 | MS.gene25468:intron | 25.0% |
| CATGTCTTATCTGCATATTT+CGG | - | chr8.4:42962903-42962922 | None:intergenic | 30.0% | |
| TTTGAAGAACTTTACAGGTT+CGG | + | chr8.4:42962981-42963000 | MS.gene25468:intron | 30.0% | |
| !! | TTAGCTTTGAAGAACTTTAC+AGG | + | chr8.4:42962976-42962995 | MS.gene25468:CDS | 30.0% |
| !!! | AGGATTTTAGGTTTTTGGAT+TGG | + | chr8.4:42963147-42963166 | MS.gene25468:intron | 30.0% |
| CGAAATATGCAGATAAGACA+TGG | + | chr8.4:42962901-42962920 | MS.gene25468:CDS | 35.0% | |
| GTTTAAGCATAGGGTTGTTA+TGG | + | chr8.4:42962875-42962894 | MS.gene25468:CDS | 35.0% | |
| ! | AGATTGAAGCGTTTAAGCAT+AGG | + | chr8.4:42962865-42962884 | MS.gene25468:CDS | 35.0% |
| ! | GATTGAAGCGTTTAAGCATA+GGG | + | chr8.4:42962866-42962885 | MS.gene25468:CDS | 35.0% |
| ! | GATTTAGAGGTGATGGTTAT+AGG | + | chr8.4:42963127-42963146 | MS.gene25468:intron | 35.0% |
| !! | GGTGATGGTTATAGGATTTT+AGG | + | chr8.4:42963135-42963154 | MS.gene25468:intron | 35.0% |
| TGGGTAAGATTTAGAGGTGA+TGG | + | chr8.4:42963120-42963139 | MS.gene25468:intron | 40.0% | |
| !! | AAAAAAAAAAATAAGAAAAA+AGG | - | chr8.4:42963012-42963031 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 42962837 | 42963241 | 42962837 | ID=MS.gene25468 |
| chr8.4 | mRNA | 42962837 | 42963241 | 42962837 | ID=MS.gene25468.t1;Parent=MS.gene25468 |
| chr8.4 | exon | 42962837 | 42962997 | 42962837 | ID=MS.gene25468.t1.exon1;Parent=MS.gene25468.t1 |
| chr8.4 | CDS | 42962837 | 42962997 | 42962837 | ID=cds.MS.gene25468.t1;Parent=MS.gene25468.t1 |
| chr8.4 | exon | 42963235 | 42963241 | 42963235 | ID=MS.gene25468.t1.exon2;Parent=MS.gene25468.t1 |
| chr8.4 | CDS | 42963235 | 42963241 | 42963235 | ID=cds.MS.gene25468.t1;Parent=MS.gene25468.t1 |
| Gene Sequence |
| Protein sequence |
