AlfalfaGEDB | MS.gene25701

M. sativa cultivar XinJiangDaYe / MS.gene25701

KEGG: K01179
GO: GO:0003674 GO:0003824 GO:0004553 GO:0005975 GO:0008150 GO:0008152 GO:0016787 GO:0016798 GO:0044238 GO:0071704
PFAM: PF00759.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25701.t1	XP_003601506.1	97	624	19	0	1	624	1	624	0.00E+00	1256.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25701.t1	Q7XUK4	62.7	630	222	8	1	624	1	623	6.3e-231	801.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25701.t1	G7J8S6	97.0	624	19	0	1	624	1	624	0.0e+00	1256.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049549	MS.gene25701	0.806183	9.31E-50	-1.69E-46
MS.gene049611	MS.gene25701	0.806846	6.74E-50	-1.69E-46
MS.gene049634	MS.gene25701	0.835312	1.81E-56	-1.69E-46
MS.gene050096	MS.gene25701	0.802817	4.69E-49	-1.69E-46
MS.gene050381	MS.gene25701	0.806178	9.33E-50	-1.69E-46
MS.gene051127	MS.gene25701	0.808485	3.03E-50	-1.69E-46
MS.gene051138	MS.gene25701	0.824914	6.23E-54	-1.69E-46
MS.gene052211	MS.gene25701	0.815115	1.09E-51	-1.69E-46
MS.gene05240	MS.gene25701	0.820372	7.10E-53	-1.69E-46
MS.gene05244	MS.gene25701	0.80649	8.02E-50	-1.69E-46
MS.gene053406	MS.gene25701	0.808991	2.36E-50	-1.69E-46
MS.gene053420	MS.gene25701	0.824122	9.58E-54	-1.69E-46
MS.gene053488	MS.gene25701	0.812519	4.07E-51	-1.69E-46
MS.gene055124	MS.gene25701	0.810224	1.28E-50	-1.69E-46
MS.gene055154	MS.gene25701	0.831396	1.71E-55	-1.69E-46
MS.gene055240	MS.gene25701	0.808059	3.73E-50	-1.69E-46
MS.gene056224	MS.gene25701	0.813994	1.93E-51	-1.69E-46
MS.gene056590	MS.gene25701	0.806894	6.59E-50	-1.69E-46
MS.gene056591	MS.gene25701	0.808359	3.22E-50	-1.69E-46
MS.gene057550	MS.gene25701	0.800199	1.61E-48	-1.69E-46
MS.gene058093	MS.gene25701	0.800714	1.27E-48	-1.69E-46
MS.gene058099	MS.gene25701	0.829996	3.76E-55	-1.69E-46
MS.gene058171	MS.gene25701	0.812028	5.21E-51	-1.69E-46
MS.gene058173	MS.gene25701	0.805229	1.48E-49	-1.69E-46
MS.gene058175	MS.gene25701	0.813969	1.96E-51	-1.69E-46
MS.gene058603	MS.gene25701	0.825686	4.10E-54	-1.69E-46
MS.gene059037	MS.gene25701	0.805979	1.03E-49	-1.69E-46
MS.gene059953	MS.gene25701	0.808974	2.38E-50	-1.69E-46
MS.gene059955	MS.gene25701	0.809773	1.60E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25701.t1	MTR_3g082440	96.955	624	19	0	1	624	1	624	0.0	1266
MS.gene25701.t1	MTR_2g028480	59.054	613	237	5	1	608	3	606	0.0	705
MS.gene25701.t1	MTR_4g128810	53.254	507	85	12	118	619	5	364	1.69e-158	459
MS.gene25701.t1	MTR_3g090540	43.038	553	297	11	66	608	57	601	6.71e-147	438
MS.gene25701.t1	MTR_3g113720	40.038	532	275	16	88	597	15	524	3.95e-104	325
MS.gene25701.t1	MTR_8g099410	40.447	492	259	15	116	596	28	496	2.35e-96	305
MS.gene25701.t1	MTR_8g099410	40.650	492	258	16	116	596	28	496	2.27e-95	306
MS.gene25701.t1	MTR_8g075990	40.862	487	254	14	117	593	64	526	1.21e-94	301
MS.gene25701.t1	MTR_5g093590	39.592	490	259	15	116	591	34	500	1.23e-93	298
MS.gene25701.t1	MTR_4g074960	38.742	493	269	14	116	597	28	498	3.52e-93	300
MS.gene25701.t1	MTR_3g080190	39.228	492	263	15	110	588	34	502	4.64e-93	296
MS.gene25701.t1	MTR_5g085730	39.014	487	255	17	116	586	31	491	8.09e-93	296
MS.gene25701.t1	MTR_3g080810	38.969	485	255	17	116	588	35	490	9.76e-93	295
MS.gene25701.t1	MTR_5g009970	38.306	496	257	14	116	598	41	500	1.69e-92	295
MS.gene25701.t1	MTR_5g010000	38.066	486	258	14	116	591	40	492	8.60e-88	282
MS.gene25701.t1	MTR_3g010330	40.329	486	244	19	116	588	25	477	5.67e-87	280
MS.gene25701.t1	MTR_3g117500	38.604	487	256	17	116	588	24	481	1.60e-86	278
MS.gene25701.t1	MTR_3g105230	36.632	475	247	16	116	576	30	464	8.89e-85	274
MS.gene25701.t1	MTR_3g023740	38.446	489	253	16	116	593	30	481	2.14e-84	273
MS.gene25701.t1	MTR_8g104820	38.398	487	257	17	116	588	30	487	1.55e-82	268
MS.gene25701.t1	MTR_1g037340	37.209	516	271	17	87	591	8	481	5.46e-81	264
MS.gene25701.t1	MTR_4g035955	39.220	487	245	18	116	588	14	463	1.75e-80	262
MS.gene25701.t1	MTR_5g009970	37.333	450	235	13	162	598	11	426	6.80e-78	254
MS.gene25701.t1	MTR_4g035930	37.681	483	258	17	116	588	15	464	4.98e-76	250
MS.gene25701.t1	MTR_4g035975	35.397	517	279	17	98	593	9	491	5.40e-76	251
MS.gene25701.t1	MTR_1g088190	37.097	496	246	17	116	596	39	483	1.31e-73	244
MS.gene25701.t1	MTR_5g009970	35.566	433	232	13	179	598	1	399	5.40e-67	224
MS.gene25701.t1	MTR_3g082460	39.683	378	119	11	246	603	3	291	1.22e-65	218
MS.gene25701.t1	MTR_5g009970	35.185	324	176	10	285	598	32	331	2.19e-48	172
MS.gene25701.t1	MTR_1g088190	39.465	299	133	12	103	393	25	283	3.04e-39	147
MS.gene25701.t1	MTR_7g108925	36.257	171	102	3	429	596	37	203	1.17e-28	114
MS.gene25701.t1	MTR_0393s0050	37.888	161	85	5	423	572	1	157	3.90e-25	103
MS.gene25701.t1	MTR_1g037320	43.023	172	80	6	179	345	1	159	8.37e-24	102
MS.gene25701.t1	MTR_1g037320	40.476	126	70	3	467	588	164	288	8.69e-20	90.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25701.t1	AT1G65610	61.085	627	237	5	1	624	1	623	0.0	773
MS.gene25701.t1	AT5G49720	58.117	616	239	9	1	608	1	605	0.0	677
MS.gene25701.t1	AT4G24260	55.663	618	256	9	1	611	1	607	0.0	620
MS.gene25701.t1	AT4G24260	53.815	498	214	7	1	492	1	488	1.43e-156	459
MS.gene25701.t1	AT4G24260	52.036	442	196	7	1	436	1	432	7.81e-132	395
MS.gene25701.t1	AT1G75680	43.644	472	236	12	117	582	58	505	1.67e-102	321
MS.gene25701.t1	AT1G19940	41.955	491	250	13	106	585	33	499	5.70e-102	320
MS.gene25701.t1	AT1G19940	41.955	491	250	13	106	585	33	499	5.70e-102	320
MS.gene25701.t1	AT1G02800	40.361	498	257	16	107	594	34	501	1.28e-92	295
MS.gene25701.t1	AT4G11050	38.023	526	280	18	116	624	26	522	2.01e-92	296
MS.gene25701.t1	AT4G02290	41.443	485	242	16	116	588	52	506	9.17e-92	293
MS.gene25701.t1	AT4G11050	38.023	526	280	18	116	624	26	522	1.21e-91	296
MS.gene25701.t1	AT4G11050	38.023	526	280	18	116	624	26	522	1.22e-91	296
MS.gene25701.t1	AT4G09740	36.122	490	269	14	116	595	23	478	7.44e-90	287
MS.gene25701.t1	AT2G32990	39.095	486	260	16	116	588	39	501	2.51e-89	287
MS.gene25701.t1	AT2G44570	37.182	511	283	14	92	591	8	491	1.55e-88	284
MS.gene25701.t1	AT4G23560	38.367	490	258	15	117	596	24	479	1.81e-88	283
MS.gene25701.t1	AT1G64390	39.059	489	264	16	116	593	25	490	8.30e-88	286
MS.gene25701.t1	AT4G39000	39.069	494	259	17	114	593	23	488	1.00e-86	279
MS.gene25701.t1	AT1G71380	39.918	486	245	19	116	588	24	475	3.07e-86	278
MS.gene25701.t1	AT3G43860	38.697	491	249	17	116	593	34	485	2.50e-85	275
MS.gene25701.t1	AT3G43860	38.697	491	249	17	116	593	34	485	2.50e-85	275
MS.gene25701.t1	AT1G22880	39.761	503	254	19	98	588	10	475	5.35e-85	274
MS.gene25701.t1	AT1G70710	37.945	506	270	17	96	588	8	482	1.31e-84	273
MS.gene25701.t1	AT4G39010	36.290	496	274	18	116	597	29	496	3.69e-83	270
MS.gene25701.t1	AT1G23210	38.193	487	255	19	116	588	28	482	5.60e-83	269
MS.gene25701.t1	AT2G44550	35.922	515	279	16	86	588	11	486	1.19e-81	266
MS.gene25701.t1	AT1G48930	38.877	481	262	13	116	586	30	488	1.48e-80	267
MS.gene25701.t1	AT4G38990	35.977	517	275	19	93	588	5	486	1.99e-80	263
MS.gene25701.t1	AT2G44540	35.853	516	282	16	86	588	11	490	2.77e-80	262
MS.gene25701.t1	AT2G44560	37.166	487	266	16	116	591	33	490	7.08e-74	245
MS.gene25701.t1	AT1G19940	39.447	398	207	12	106	492	33	407	1.07e-68	229
MS.gene25701.t1	AT4G02290	39.100	422	217	15	179	588	1	394	4.73e-68	227
MS.gene25701.t1	AT1G22880	37.915	422	219	17	179	588	1	391	2.23e-64	218
MS.gene25701.t1	AT2G44570	34.515	423	243	12	92	507	8	403	1.57e-55	194
MS.gene25701.t1	AT4G39010	34.367	387	219	15	116	493	29	389	2.30e-54	190
MS.gene25701.t1	AT1G22880	40.964	332	157	15	98	423	10	308	3.09e-45	164
MS.gene25701.t1	AT4G38990	33.245	379	193	14	93	464	5	330	3.97e-44	161

Find 151 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGTCTTCTTCTTCATATT+TGG	0.162977	3.3:+69338465	None:intergenic
AAGACCAAGTATGTGGATTT+TGG	0.214969	3.3:-69338450	MS.gene25701:CDS
CATGAAATTCCATTGCTCTT+TGG	0.243720	3.3:+69332663	None:intergenic
ATGCCAGAGAACTCATTAAA+TGG	0.258995	3.3:-69332458	MS.gene25701:CDS
TAACCAGTTGAGTTATAAAA+AGG	0.260842	3.3:+69331757	None:intergenic
ATCAATTCAATGTCTTTAAC+TGG	0.265648	3.3:-69331462	MS.gene25701:CDS
ACAGATATTGGAAAGAAATT+AGG	0.286784	3.3:+69331198	None:intergenic
CGTGGATAGCTCATGTCTTC+TGG	0.287615	3.3:+69331973	None:intergenic
AATGCCTTTAGAACAGATAT+TGG	0.289238	3.3:+69331186	None:intergenic
CTGTAATGTTGTTAGGATTT+CGG	0.297943	3.3:+69330545	None:intergenic
TGTGCTTGTTAAGGAAATTA+AGG	0.304790	3.3:+69330417	None:intergenic
ATTGCTCCTGTAATGTTGTT+AGG	0.311527	3.3:+69330538	None:intergenic
TGAAATTGGTCAAAACGATT+TGG	0.351632	3.3:+69330505	None:intergenic
TTTGAAGCTATTCCACCATT+TGG	0.352217	3.3:-69330364	MS.gene25701:CDS
TTTGAAGGACCTGACCTTGC+AGG	0.353162	3.3:-69331934	MS.gene25701:CDS
ATGGAAGCCAAAGGTGTTCC+TGG	0.353530	3.3:-69331234	MS.gene25701:CDS
TTCCAAGGAGGTGGTGGAGG+TGG	0.363591	3.3:+69330331	None:intergenic
CGAAATCCTAACAACATTAC+AGG	0.366600	3.3:-69330544	MS.gene25701:CDS
GTGGATCACTGAATGGTTCT+AGG	0.369729	3.3:-69332026	MS.gene25701:CDS
TAATCCTGGTTACCCTTATG+AGG	0.370170	3.3:-69331539	MS.gene25701:CDS
ATGTTGTTAGGATTTCGGTT+AGG	0.371448	3.3:+69330550	None:intergenic
TGTTGTTAGGATTTCGGTTA+GGG	0.380281	3.3:+69330551	None:intergenic
TTGACCAATTTCATGATTCA+AGG	0.383673	3.3:-69330495	MS.gene25701:CDS
TAAAATGCCTTGGCATTCTT+AGG	0.383795	3.3:+69331646	None:intergenic
TGCCAACAGTAGTGATCATC+TGG	0.384464	3.3:+69332000	None:intergenic
TTGCTATGACAATGCTAAGT+TGG	0.392718	3.3:-69332530	MS.gene25701:CDS
ACTATGTAGCTCATGTTCAT+TGG	0.395336	3.3:+69330679	None:intergenic
TGCCAGAGAACTCATTAAAT+GGG	0.398898	3.3:-69332457	MS.gene25701:CDS
TGGTAAGACTAGTAACATTA+TGG	0.398981	3.3:+69331500	None:intergenic
ATGCCGCATTGACAACATAT+TGG	0.399475	3.3:+69331283	None:intergenic
GCCATTTCTCCTGCAAGGTC+AGG	0.400536	3.3:+69331925	None:intergenic
ATGAGCTACATAGTAGGATA+TGG	0.410733	3.3:-69330670	MS.gene25701:CDS
TTGGCATTCTTAGGCACATC+AGG	0.410800	3.3:+69331655	None:intergenic
CTTTCCAATATCTGTTCTAA+AGG	0.414260	3.3:-69331190	MS.gene25701:CDS
TATACTGAACCAACTTTAGC+TGG	0.415273	3.3:-69330460	MS.gene25701:CDS
GTTAGGATGTTTCTTAATCC+TGG	0.415763	3.3:-69331553	MS.gene25701:CDS
ACCTGACCTTGCAGGAGAAA+TGG	0.419662	3.3:-69331926	MS.gene25701:CDS
ATGCTAAGCATATCCTCATA+AGG	0.426193	3.3:+69331526	None:intergenic
CCATTGATCTCCGGTGCGGC+TGG	0.426847	3.3:+69338566	None:intergenic
GGTTTCCAAGGAGGTGGTGG+AGG	0.427321	3.3:+69330328	None:intergenic
TTTGCATTTGCAAGAGACTT+TGG	0.429572	3.3:-69331820	MS.gene25701:CDS
CAAAGAGCAATGGAATTTCA+TGG	0.434088	3.3:-69332662	MS.gene25701:CDS
AATTACTCATCATGCATTCA+AGG	0.437438	3.3:-69338650	None:intergenic
ACTACCTGTTGTGCTTGTTA+AGG	0.440011	3.3:+69330408	None:intergenic
TGTTGCTTCTAACAAGGGTT+AGG	0.440413	3.3:-69331570	MS.gene25701:CDS
AGAGCAAGTGTGTAATTATC+AGG	0.442800	3.3:+69338300	None:intergenic
ATCCACCTCCACCACCTCCT+TGG	0.444399	3.3:-69330333	MS.gene25701:CDS
CCTTCAAATACAGTCGTTGT+TGG	0.444436	3.3:+69331949	None:intergenic
CAGCCGCACCGGAGATCAAT+GGG	0.447656	3.3:-69338565	MS.gene25701:CDS
AAAACCTTGGCAATGATGAT+AGG	0.448092	3.3:+69338351	None:intergenic
CTTCAAATACAGTCGTTGTT+GGG	0.448957	3.3:+69331950	None:intergenic
AATACAGTCGTTGTTGGGCG+TGG	0.450894	3.3:+69331955	None:intergenic
TCATGCATTCAAGGAATCAA+TGG	0.452562	3.3:-69338641	MS.gene25701:CDS
AATGTCTTTAACTGGACTAA+AGG	0.453410	3.3:-69331454	MS.gene25701:intron
TGGTAATCGACGTGAGGGAT+TGG	0.455806	3.3:-69338520	MS.gene25701:CDS
CAACTCAAAACACTCAAATC+AGG	0.456687	3.3:+69331616	None:intergenic
AAATTCTACCTACATTTCCT+TGG	0.457242	3.3:-69331686	MS.gene25701:CDS
GTAGGTGAAGAATGATGTTT+AGG	0.458364	3.3:+69338327	None:intergenic
TGGACGAGACATTTCAAAGC+TGG	0.461650	3.3:-69338500	MS.gene25701:CDS
ATACCATCCAGGAACACCTT+TGG	0.461703	3.3:+69331227	None:intergenic
TGCCTTGTCCCATTGATCTC+CGG	0.462338	3.3:+69338557	None:intergenic
CCTCTATGATGAACATGTCT+TGG	0.462506	3.3:+69330637	None:intergenic
ATTTCCTTAACAAGCACAAC+AGG	0.462832	3.3:-69330412	MS.gene25701:CDS
ACCCCATTTAATGAGTTCTC+TGG	0.464185	3.3:+69332455	None:intergenic
TGAGTCACAAGGCATTGAAA+TGG	0.465887	3.3:-69338416	MS.gene25701:CDS
TATTAACAAAATTTATGCTC+AGG	0.465959	3.3:-69332391	MS.gene25701:intron
AAAGGCATTTGCAACCTCTC+AGG	0.468135	3.3:-69331172	MS.gene25701:intron
CTGCAATGTTGCTTCTAACA+AGG	0.469739	3.3:-69331576	MS.gene25701:CDS
CATGCATTCAAGGAATCAAT+GGG	0.471241	3.3:-69338640	MS.gene25701:CDS
AGTACATGTGGGGTGGTGCT+TGG	0.473838	3.3:-69331726	MS.gene25701:CDS
TAAACAGATGGATTACATCA+TGG	0.476747	3.3:-69330710	MS.gene25701:intron
TGCTAAGCATATCCTCATAA+GGG	0.477600	3.3:+69331527	None:intergenic
CCAGCCGCACCGGAGATCAA+TGG	0.478807	3.3:-69338566	MS.gene25701:CDS
CATGGTTTCCAAGGAGGTGG+TGG	0.493242	3.3:+69330325	None:intergenic
TTGAATAAGCAGCATCGTCT+AGG	0.501483	3.3:+69331872	None:intergenic
CCTGCATTTCCAGCTAAAGT+TGG	0.502786	3.3:+69330451	None:intergenic
CCTTCCTATCATCATTGCCA+AGG	0.506351	3.3:-69338355	MS.gene25701:CDS
GGCGATGAGACCAGCCGCAC+CGG	0.506587	3.3:-69338576	MS.gene25701:CDS
CCAACAACGACTGTATTTGA+AGG	0.513357	3.3:-69331949	MS.gene25701:CDS
GGAGAGATTGTGGTTGTCCA+TGG	0.513687	3.3:+69331304	None:intergenic
AAGGCAGCGTTGCTCGATCA+TGG	0.514359	3.3:-69338540	MS.gene25701:CDS
CCAAGACATGTTCATCATAG+AGG	0.514706	3.3:-69330637	MS.gene25701:CDS
TGAAATTCATGTTAGGTTGG+TGG	0.514876	3.3:-69332045	MS.gene25701:intron
ATGGGATGCAGAACTGTTGA+AGG	0.514883	3.3:+69332413	None:intergenic
ATTACTCATGTACTGGTGGT+TGG	0.515779	3.3:-69330588	MS.gene25701:CDS
GTTGGTGGTTATTATGATGC+AGG	0.518239	3.3:-69332585	MS.gene25701:CDS
CCAACTTTAGCTGGAAATGC+AGG	0.519047	3.3:-69330451	MS.gene25701:CDS
CAGCAATAGTGAAGTGCCAT+CGG	0.520237	3.3:-69338598	MS.gene25701:CDS
TTCAAAGCTGGATTCTTGAA+AGG	0.521917	3.3:-69338488	MS.gene25701:CDS
GTTAGAAGCAACATTGCAGC+AGG	0.524873	3.3:+69331580	None:intergenic
AGCAATGGAATTTCATGGAG+AGG	0.525294	3.3:-69332657	MS.gene25701:CDS
ATGAACATGAGCTACATAGT+AGG	0.525898	3.3:-69330676	MS.gene25701:CDS
CATGTACTGGTGGTTGGAAG+TGG	0.534773	3.3:-69330582	MS.gene25701:CDS
GCATCCAAAATCCACATACT+TGG	0.535254	3.3:+69338446	None:intergenic
AGCAATAGTGAAGTGCCATC+GGG	0.538979	3.3:-69338597	MS.gene25701:CDS
AGGCTTCATATAAAATGCCT+TGG	0.543329	3.3:+69331636	None:intergenic
ATTAGTTGCCAAGGAAATGT+AGG	0.544392	3.3:+69331678	None:intergenic
GGGAACTCAGGTTTACAAGA+TGG	0.549155	3.3:-69332636	MS.gene25701:CDS
AAACAGATGGATTACATCAT+GGG	0.556675	3.3:-69330709	MS.gene25701:intron
AACATTACAGGAGCAATGGT+TGG	0.556744	3.3:-69330532	MS.gene25701:CDS
GAATGATATCATGGTTTCCA+AGG	0.560598	3.3:+69330316	None:intergenic
TAGGTTGGTGGATCACTGAA+TGG	0.562722	3.3:-69332033	MS.gene25701:CDS
CACCGGAGATCAATGGGACA+AGG	0.566153	3.3:-69338559	MS.gene25701:CDS
TTAACAAGCACAACAGGTAG+TGG	0.568632	3.3:-69330406	MS.gene25701:CDS
CGGCTGGTCTCATCGCCCGA+TGG	0.570578	3.3:+69338582	None:intergenic
GAAGAAGAAGACCAAGTATG+TGG	0.572331	3.3:-69338457	MS.gene25701:CDS
ATTTCATGGAGAGGGAACTC+AGG	0.573575	3.3:-69332648	MS.gene25701:CDS
CCTTGGCAATGATGATAGGA+AGG	0.574607	3.3:+69338355	None:intergenic
GCTGATTATATGGAAGCCAA+AGG	0.576136	3.3:-69331243	MS.gene25701:CDS
GCAATGGAATTTCATGGAGA+GGG	0.579923	3.3:-69332656	MS.gene25701:CDS
CATTGTCATAGCAAAAGACA+TGG	0.581404	3.3:+69332539	None:intergenic
GGGAGATTGCCAAAGAGCAA+TGG	0.582582	3.3:-69332672	MS.gene25701:CDS
CACATCAGGATTAGTTGCCA+AGG	0.583526	3.3:+69331669	None:intergenic
ACAACATATTGGAGAGATTG+TGG	0.586233	3.3:+69331294	None:intergenic
ATGATGTTTAGGCAAAACCT+TGG	0.586680	3.3:+69338338	None:intergenic
CTGCTGCCATTTCTCCTGCA+AGG	0.587328	3.3:+69331920	None:intergenic
AAGCCAAAGGTGTTCCTGGA+TGG	0.590814	3.3:-69331230	MS.gene25701:CDS
TGCAATGTTGCTTCTAACAA+GGG	0.591784	3.3:-69331575	MS.gene25701:CDS
CACCAGATGATCACTACTGT+TGG	0.591909	3.3:-69332002	MS.gene25701:CDS
TATCATGGTTTCCAAGGAGG+TGG	0.593765	3.3:+69330322	None:intergenic
ATTACAGGAGCAATGGTTGG+AGG	0.593896	3.3:-69330529	MS.gene25701:CDS
GGTGTTCCTGGATGGTATTG+TGG	0.602884	3.3:-69331222	MS.gene25701:CDS
TAACAACATTACAGGAGCAA+TGG	0.606000	3.3:-69330536	MS.gene25701:CDS
GTGTAATTATCAGGTGGTGT+AGG	0.609113	3.3:+69338309	None:intergenic
ATATCCTCATAAGGGTAACC+AGG	0.610242	3.3:+69331535	None:intergenic
TATTCAAAGAAACTCGTCAA+GGG	0.616213	3.3:-69331856	MS.gene25701:CDS
TTAGGACCACAATACCATCC+AGG	0.617520	3.3:+69331216	None:intergenic
AAAGTCTCTTGCAAATGCAA+AGG	0.621930	3.3:+69331822	None:intergenic
CGATCATGGTAATCGACGTG+AGG	0.629064	3.3:-69338526	MS.gene25701:CDS
GTTACTTTGATGAGTACATG+TGG	0.631061	3.3:-69331738	MS.gene25701:CDS
CATTCAAGGAATCAATGGGG+AGG	0.631381	3.3:-69338636	MS.gene25701:CDS
TACTTTGATGAGTACATGTG+GGG	0.640760	3.3:-69331736	MS.gene25701:CDS
TGATATCATGGTTTCCAAGG+AGG	0.640965	3.3:+69330319	None:intergenic
ATTCAAGGAATCAATGGGGA+GGG	0.642541	3.3:-69338635	MS.gene25701:CDS
TTATTCAAAGAAACTCGTCA+AGG	0.644851	3.3:-69331857	MS.gene25701:CDS
GATCATGGTAATCGACGTGA+GGG	0.648711	3.3:-69338525	MS.gene25701:CDS
TTTGATGAGTACATGTGGGG+TGG	0.652316	3.3:-69331733	MS.gene25701:CDS
TGATGTGCCTAAGAATGCCA+AGG	0.657170	3.3:-69331653	MS.gene25701:CDS
GCCAGAGAACTCATTAAATG+GGG	0.671647	3.3:-69332456	MS.gene25701:CDS
ATTGTCATAGCAAAAGACAT+GGG	0.674505	3.3:+69332540	None:intergenic
ATGCATCGTGTTGAGTCACA+AGG	0.674798	3.3:-69338427	MS.gene25701:CDS
TTACTTTGATGAGTACATGT+GGG	0.676107	3.3:-69331737	MS.gene25701:CDS
GCAAGTGTGTAATTATCAGG+TGG	0.676351	3.3:+69338303	None:intergenic
AAAAGATCAACATACAGTCG+CGG	0.678960	3.3:-69331796	MS.gene25701:CDS
TTGATGAAGATAAGAACACA+TGG	0.698282	3.3:+69331480	None:intergenic
GGGTTGATACAATTGAACCA+TGG	0.709415	3.3:-69331321	MS.gene25701:CDS
AATGTAGGTAGAATTTCCAG+TGG	0.715370	3.3:+69331693	None:intergenic
TGTCCCATTGATCTCCGGTG+CGG	0.718263	3.3:+69338562	None:intergenic
TCTCCAATATGTTGTCAATG+CGG	0.724183	3.3:-69331286	MS.gene25701:CDS
TGAAGAATTTGGTACCTGAG+AGG	0.761751	3.3:+69331158	None:intergenic
ATGCATTCAAGGAATCAATG+GGG	0.776623	3.3:-69338639	MS.gene25701:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	69330324	69338661	69330324	ID=MS.gene25701
chr3.3	mRNA	69330324	69338661	69330324	ID=MS.gene25701.t1;Parent=MS.gene25701
chr3.3	exon	69338265	69338661	69338265	ID=MS.gene25701.t1.exon1;Parent=MS.gene25701.t1
chr3.3	CDS	69338265	69338661	69338265	ID=cds.MS.gene25701.t1;Parent=MS.gene25701.t1
chr3.3	exon	69332392	69332696	69332392	ID=MS.gene25701.t1.exon2;Parent=MS.gene25701.t1
chr3.3	CDS	69332392	69332696	69332392	ID=cds.MS.gene25701.t1;Parent=MS.gene25701.t1
chr3.3	exon	69331455	69332052	69331455	ID=MS.gene25701.t1.exon3;Parent=MS.gene25701.t1
chr3.3	CDS	69331455	69332052	69331455	ID=cds.MS.gene25701.t1;Parent=MS.gene25701.t1
chr3.3	exon	69331173	69331345	69331173	ID=MS.gene25701.t1.exon4;Parent=MS.gene25701.t1
chr3.3	CDS	69331173	69331345	69331173	ID=cds.MS.gene25701.t1;Parent=MS.gene25701.t1
chr3.3	exon	69330324	69330725	69330324	ID=MS.gene25701.t1.exon5;Parent=MS.gene25701.t1
chr3.3	CDS	69330324	69330725	69330324	ID=cds.MS.gene25701.t1;Parent=MS.gene25701.t1

Gene Sequence

>MS.gene25701

ATGCATTCAAGGAATCAATGGGGAGGGTCTTTTGAGCTGAGCAGCAATAGTGAAGTGCCATCGGGCGATGAGACCAGCCGCACCGGAGATCAATGGGACAAGGCAGCGTTGCTCGATCATGGTAATCGACGTGAGGGATTGGACGAGACATTTCAAAGCTGGATTCTTGAAAGGCCAAATATGAAGAAGAAGACCAAGTATGTGGATTTTGGATGCATCGTGTTGAGTCACAAGGCATTGAAATGGATTTTTGGTACAATCTTTGTAGGTTTTTGTGTAATTGGCCTTCCTATCATCATTGCCAAGGTTTTGCCTAAACATCATTCTTCACCTACACCACCTGATAATTACACACTTGCTCTTCACAAAGCGCTACGCTTCTTTAATGCTCAGAAATGTATGTAATCTTCTACTATCTCATATCATCATGCAAATTAACATTCACATATATTTTCTCTGTGTGCATTACTCTGTTCTCATTTGGTTGGTCTCATTTGACATTTTACTTTTTCTCCCATGATTTTATTTGATTTATTAGATGATTGATACACTCAATATATTTTTTAGGTGAAAGTGATAGACTCAATATATGATAATATAAGATCCTTAGATAAATAAAAATAGAGTAAGACCCTTTCAAAATGTTGTTTGCTTTCATATGCATATATGCTTTTGAATAGGCGTGGTTTTGGTTGTTATGACTTTTGCTGAAATATGTGCCTCTAGTTAATAAAGATGTTAATGTTAATAAATAAAATTATTCACTTGCTATATTATGGAATTGACTAATAACAAGATTTGACTTAAGTGTTAATATCATAGATCATCTTAAAGGACATGCTTGATTGATAATTGAGTTTGATTTTTGATAAAAAAATTGTAATTAAACTTTACTTTTTTTTGGATCAAGTAGTTTAATGGATAATAATTTACCTTTATGGGGAATAAACTGAAAATCCAGAGTTTAAACCCGACCCTTGAATATATAATGCAAATTTCTTACCAATTGAGTTAAGTTTATGATGACGTAATTAAACTTTACTTATTCTACGATCAAATACAACTATCCTAAATACGATTACCCTTAATATTATATAAGTGAAAAACATAATTACATAAAAAATAACCAATGAAATACAATATATAATATTTATTTATTTATTTATTTACTTATCCTTCTGTAGAATTTATTTATTTTTGACAAAATTTTCTTCTGTAGATACTATTATACAATGTTAATATAATATATAATATAATATAACTGCTCTCTTTGATATATTCATATTTTTTTTGAAGGATTGATATATTCATATGAGAAATGATATTTTGACACTCACTTTTTGACAACTTTTGAACAACTTTCTCTCTCATATTCACATGATGTTGTTATCCTCTCTCTTCTTTTTTCTCTCTCCATTGTTTTTGACCAATAAGAAGTGAGAAAAATAAGGTTGTCAACAAAGTTGTCATCAAATGGATGTCAAAATATCATTACTCTATTCATATTTGTTTTCTTACAAAAGGGAATGAAGCATTTGTTGTCCCCTTTTACCCGTCAACTCCTTCCAAAGGCTGGTTCAATAAGCCCCAAAAAAACATATTTTAACTAACTCTCTGCATCTGCATATTATCTCAAATTTCAAAATAATAAAATTATGCAACTTTTATACGTGAGATAAACAATTTGGGTGTGTTTGGATTAGCAATTTAATTAAGCACTTGTTGAGTTAGTATCTGACACGTGTGTTTATGTAAGTTTTTTTTTTATAATAATAAAATAATAAGGTTAATATTTTTCATATAAGTCGCAAGTTACTAAGCTATATACACATTTTCCTTCAAAAAAAAAAAAGCTATATACACATTCACACACGTGTATATATAAAACCATCATCTTACAAATGCACTTTTATTGTTTTACTAAAACTTTTTGATGTTTAAATCCTCTGAAACTGTGCTTATGGAAAATCCGCTTAGGAGATTTTGTGTCTAACATTACTGTTAGACTCTTTCTAATAGTAATTTAGAGTATTGATTAACAAATAAAAATATTAAAATAAAAATATAGTTGACAAACCTTTTCATTTTTTTTATACTAGTACAATTTTTGAACTAATATGATTAAGACAGTAATCGTTCTTACCATTTTATTATTGCCAAATGCTATCTAAGGTCTTTTCATTCCAACAAGCTGCTTTGAATTCCTTTAAATAATTTCATCCCATAGTTCTTGACTCTTTCAAATAAAAAATGATATCAACAGGACGGCATTTAGCACGAATGTGTTTGTCTTGTGTCGCTAGTATTTGAATGGTTCTGTTTGTATCCCGTTTTCATTCCATTCTTATTTTGTTTCACGATAACACACGCTTTATATGTTTTGGTGATAAATAAATAAATAATATACAAATTAATATTAATAAATAAATAAATTAGTCCTTCTATTAATTTTGGTTATAACAAATTGACTGCTTTGTCTTTACTCTTTATATGAAAATGTTCAGATTTAGTTGGCCATTAATTTTTGCTAATAACAACAAAATTGAGAGGAAATATATAGATTATTTTGTCGAGCATTGCTATTTATCTAGAGAGTTTTTATCAAGAAAAGGTCTCAACCAATTAAATTTGTTGTTTGACGGAAATCAAGGGAGGTAATGGTGTGTAATTAAGGAAAAGTGTTAGCAAATACATTGTCTTGAAGTTTTCTTGATAAATTTTGTCACCGTATCTATCATTTCCCTTATTTTGTCCAAAAAATAGTTAATACATCGCACTTAACACTTAGAAACATAACAAGTGTCCCCTTCAGGGACTTCCGATAAAATTGGATCGATACATAGATACATAACAAGTATTTTTTGAATTTAAAGTATATTTAGTTTTCTAGTTATTGTTGGTTGCTATCATAAAACATCAATATAAATTTATCTCAATTTTTTGTTGCTTGATGAGTTTAGTGGCGAAACTCACAAATACCAATTGTTGAGAAGTAGAGGATTACACTTTCGAACTATTAGAGATATATAGTTTAGTTACCACTAGTATTAGTCCCACTAGTATATATAGGTGTAACCTCCCCATAAGTGGGTCAACTCTCTCATATTGTAACATTTCATTTTCCATCAATAGAAAGAATTCACAAGCTTTTCTCTCCTTCATCAATGGATGCCATGTTTCATAACATGGTATCAGAGCAATCTTGGTATCCACCTTGAAGGATATTTTCCGCTGCATTTTCTTGAGAAAATTCAATTTTCTCACTTTTATTTTTTTCCTTTCTCGTTCCAATTTTCTTTTTCTCCTTTCACACTCCTTCGTTTTCTGTTGTTCTTGATTCTGTTACAATCTCAACAATGAGATTCAGTTTTCTTTCTCATCACCTTTATCCTGTAATCGAATCCGTTTTCTATTATTGATTTGTTCGATCGATTTCTCATTCGATCCGCATCTCAATTTCATTCGATTCCTTTACATTTTTTATCGATCGATTGCTTCGTCATTCTGTTTGTGTCTTTTTCCCAAATTTGCGATTCAGTTACAATCGCGATTACCTTTCTCTCACGATCCTTTGTTCAATTCGCAATTCGAATTCAGTTTCGCTTTTCAAATTCTATACAGATTTCATTCTCAACACTTGCAGAAGTGCTTTTGATTCAAATTTGGAAAAAGTGTGTTTCAGATAAGATGTTTCAGAAAAGCGCGTTTTCCATAAGCTGTTACACCAGTGGATCATCATCTTCCACCAGTGGATCATCATCTTCCACCATGGAAATAGATTTGGACATTTCTCAACCTGGTTCTGTTTTGTGAAGTGCTTTTAGGAGGAGTAATTTCGGTTCAGATTTTGGAATCGCGTTTCTGGATGTCTTCATGTTTCTACTTCACTTTCTGTGATTGATCCAGTTGTTGTGATGGATTTCACCTTGATCAGCTTCATTCGTACCTTGCATATTGCTTCCAAGGTGTTTGTTGAAAGTCCTCAATGGAAGACTTATTGGTTTTTGCTCAGAAGTTATTTGTTCAGCTGGTATATGATGAAACTTCTTGTACAAATCAACTTTGTCAGCTTCCTTTAGTGCATTGGTTTCTCAATTTGGTACATTGATCCTCCCTTTGGTTGTGTATATGCTGACTTACGAGAGGTATCTTCTTATACTTGTGGATTGCCAATTATTGGTTTGGAGATACATTTGTGAAACTTGGTTGGTGTATAGCATGGGAATCATACTTTCTTATACACACCTTTGGAACTTTGATCTATGTTGGTTATTGCTTATGGTGAACTTAGATGCTGAATACATGGCCTTATGTAGAATTGTAATTATGGCTTATTGCAATTTCAACATATCTTGTTGGCAAGTTACATTCAGTTTTTAGCCATCTATCATTGTGGACCTAAGGCACTTTTTCAAGAGATTTTATCGAAGTGTATGGTGTAAGATTGAATGGTATTGCATCTGCATCTCCCTTATACGCCTTAAGTCTATGTGGCTTTCTTCATCAGTTTTGGCAGATTGTCTTTAACACTTGGCTCCCTTCTTTTATGCGTTCTACATGGCATTTTCATGTCCTTGGTGGTGAGGTTTATGATTCTGGCTCAGTTTTGGATTGTTGGAAGATTCTTGGATTGATTACTTTTCCATGGTTTCTTTGTCCTGGATCGTTGTTTGGAGTGGTTGCTTTCTGCAGCTTTGTGATTTGGCCTATTATTTGTTTCTGGCTATTGTGACAATTGAAGACTATTTTTTGGATTTGGTTTAGCTTCATTATTGGAAGCATTGGTTTTGGCCTATGGTTTGAATGCATCAACATGGATTTGTTTTCTATGGTTTGAAGACACATCACATGGCTATGCATCTCTATTGGTTTGCCATTGGTTTTTTTTGTCTCGGCTTGGCATAGGAGGGCATCACTTTTATCTTTGGAAGCTTGGTTTTGTTGCTTCATGGTCCTTTTGGTGTGAAGTTTGTTCAACATTTGACCTCCTTCCATTGTTGACTATGGCCCTTCCTTGCATTTGGTTTTTTCTTGTCTCGGCTCGGCATTGGAAGGGCACCACTTTTATCTTTGGAACCTTGGTCTTGTATATGACCTCCTTCCATTGTAGACTATGGCTCTTCCTTGCACTTCTTTTTGGATATCATCATTGATTTTGGATATCCTTGTTCTTCTTGTCTTGTGCTTCTATCTTGAGTTCTTGAGAACTCTCGATGGTTAAGCGTTGTGCCTCAATTGGCTTTGTTGATATTGGCTTCTACCGGATGGTGGTTAAGCGTTGCTTGGTTGCTTCTTCCTTCAATTGGTTCAACCTTGAGTTGTGGTTGCTTCTTGTTGGATAAGCATTTGTTTGATGATTTTTTTTAGCCGATGTATTTCCGGCATCTTTCCTTGTATTTTCTCACTTCAAGTTGGAAGTGTTTTGAACACCTTCCAACTTGAGGGGGACTATTAGAGATATATAGTTTAGTTACCACTAGTATTAGTCCCACTAGTATATATAGGTGTAACCTCCCCATAAGTGGGTCAACTCTCTCATATTGTAACATTTCATTTTCCATCAATAGAAAGAATTCACAAGCTTTTCTCTCCTTCATCAATGGATGCCATGTTTCATAACACGAACCTACACCACAACATATCAAATATCCATGCAACCTTTTATGACATGAAATGAATTTACAAGACAGTTTATATCTGTTTTTGACACTACTACTACATATTATGTTTCAATGTTTTTAAAATAAACTTGTACATGCACAATAATAACTAGCCTTTAATAAGATGATAATTGCACCATATGTTAAAAATGTACTAGTAACAATACCTTACAAAAATAATTTGAATCATGAATCATCTAGAGAGTAAAGTCTAAGTGAAAAATTAAGGGCTACTTATTTAAACATATTCATCCATGATTTTTAATGATGTAACTTTATATAACATGTACCTTTGATTTGTGAAGCTGGGAGATTGCCAAAGAGCAATGGAATTTCATGGAGAGGGAACTCAGGTTTACAAGATGGAAAAGACACCGATGATAAAAAAGGACTTGTTGGTGGTTATTATGATGCAGGAGACAATATAAAGTTTCATTTTCCCATGTCTTTTGCTATGACAATGCTAAGTTGGAGTGTGCTTGAATATAAACAAAAATACATGGCAGTGAATGAGTATGATCATGCCAGAGAACTCATTAAATGGGGTACTGATTATTTGCTCTTAACCTTCAACAGTTCTGCATCCCATATTAACAAAATTTATGCTCAGGTAAGTTAAGTCTTGTTTCTATATATTTTATTCTTAATCAATTAATCATATGTATGTATTAGCCCTATTGAAGAAGTCGCGTATTCATATAAAAACTCTTCATTATTTGATGCATTACCCTTAAATATTCCAACTTTCATATACAATATCAAGGAAAATGTCGTTCAAACATTCGTCTCTACATATTTTTCTGTCACCAATTTTAGTCTTTGCCATTACTACAGTGACATCTGTCGAACAGACTAGACGAGTGTCACATGACTTTTTTTTTTGATGATGTGACATTATTGAGATCATAAATTTGGATCCTAAGAGCAATACATCTGAAATTCATGTTAGGTTGGTGGATCACTGAATGGTTCTAGGACACCAGATGATCACTACTGTTGGCAAAAACCAGAAGACATGAGCTATCCACGCCCAACAACGACTGTATTTGAAGGACCTGACCTTGCAGGAGAAATGGCAGCAGCATTAGCAGCAGCTTCTATAGTTTTCCTAGACGATGCTGCTTATTCAAAGAAACTCGTCAAGGGAGCTGAAACTGCCTTTGCATTTGCAAGAGACTTTGGTAAAAGATCAACATACAGTCGCGGAAAGCCTTACATTGAACCTTTTTATAACTCAACTGGTTACTTTGATGAGTACATGTGGGGTGGTGCTTGGTTGTATTTTGCCACTGGAAATTCTACCTACATTTCCTTGGCAACTAATCCTGATGTGCCTAAGAATGCCAAGGCATTTTATATGAAGCCTGATTTGAGTGTTTTGAGTTGGGATAATAAGTTACCTGCTGCAATGTTGCTTCTAACAAGGGTTAGGATGTTTCTTAATCCTGGTTACCCTTATGAGGATATGCTTAGCATGTACCATAATGTTACTAGTCTTACCATGTGTTCTTATCTTCATCAATTCAATGTCTTTAACTGGACTAAAGGTAATTCAATAATTAACATGTTACTTTCGCCTTGTTTTTTTATTCAAAATATTACGATTTTGAAGTTTCAACAAAATCATAAGTTGCATGTTAACATGAAAATTTGCAGGGGGGTTGATACAATTGAACCATGGACAACCACAATCTCTCCAATATGTTGTCAATGCGGCATTTTTGGCTTCTCTTTTTGCTGATTATATGGAAGCCAAAGGTGTTCCTGGATGGTATTGTGGTCCTAATTTCTTTCCAATATCTGTTCTAAAGGCATTTGCAACCTCTCAGGTACCAAATTCTTCACATCATTTCGAATTTTGATTCATGTATTTTTGTCAGTTGTTTTATTTGAACAGTCATATTTTGACAACTTTCGCAACAACTTTCGAAGTGTTTAACATAGTTTAAATAATTGGTTAATTACGTCTGGTAAATGGTCAATGGAAATACCAGGTGTTGAAACTCATTAACCACAAACGTAACTTCATTAAACAGGATTTTGGAACCATAATTCTCAGTGCTTAACTATACATATAGTTGAACACCTGAAAATTATGGTATACCACTCATTTTCAGGGGTATACTGATTAAGCATCTATTAAACATAATTAACCACTTATTTAAACTGTGTTAACCGCTTCAAAAGTTGTCACAAATTGTCTAATTAAGTCATACAAAATCATTTCTGTTTTTGTTCTTTAGCAATGTGATTTTTCTTATATTTCCATTAAACAGATGGATTACATCATGGGTAAAAATCCAATGAACATGAGCTACATAGTAGGATATGGCAAAAAGTTTCCAAGACATGTTCATCATAGAGGTGCTTCGATTCCAAATGATCACAAAAATTACTCATGTACTGGTGGTTGGAAGTGGCGTGACAGCCCTAACCGAAATCCTAACAACATTACAGGAGCAATGGTTGGAGGACCAAATCGTTTTGACCAATTTCATGATTCAAGGAAAAATTACAACTATACTGAACCAACTTTAGCTGGAAATGCAGGACTAGTAGCTGCCTTAATTTCCTTAACAAGCACAACAGGTAGTGGCATTGACGTTAATACCATTTTTGAAGCTATTCCACCATTTGGACCAGAAAATCCACCTCCACCACCTCCTTGGAAACCATGA

Protein sequence

>MS.gene25701.t1

MHSRNQWGGSFELSSNSEVPSGDETSRTGDQWDKAALLDHGNRREGLDETFQSWILERPNMKKKTKYVDFGCIVLSHKALKWIFGTIFVGFCVIGLPIIIAKVLPKHHSSPTPPDNYTLALHKALRFFNAQKSGRLPKSNGISWRGNSGLQDGKDTDDKKGLVGGYYDAGDNIKFHFPMSFAMTMLSWSVLEYKQKYMAVNEYDHARELIKWGTDYLLLTFNSSASHINKIYAQVGGSLNGSRTPDDHYCWQKPEDMSYPRPTTTVFEGPDLAGEMAAALAAASIVFLDDAAYSKKLVKGAETAFAFARDFGKRSTYSRGKPYIEPFYNSTGYFDEYMWGGAWLYFATGNSTYISLATNPDVPKNAKAFYMKPDLSVLSWDNKLPAAMLLLTRVRMFLNPGYPYEDMLSMYHNVTSLTMCSYLHQFNVFNWTKGGLIQLNHGQPQSLQYVVNAAFLASLFADYMEAKGVPGWYCGPNFFPISVLKAFATSQMDYIMGKNPMNMSYIVGYGKKFPRHVHHRGASIPNDHKNYSCTGGWKWRDSPNRNPNNITGAMVGGPNRFDQFHDSRKNYNYTEPTLAGNAGLVAALISLTSTTGSGIDVNTIFEAIPPFGPENPPPPPPWKP