Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25703.t1 | RHN69057.1 | 76.8 | 138 | 32 | 0 | 1 | 138 | 105 | 242 | 1.60E-53 | 218.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25703.t1 | G7J851 | 76.8 | 138 | 32 | 0 | 1 | 138 | 86 | 223 | 1.2e-53 | 218.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene25703 | MS.gene70476 | PPI |
| MS.gene25703 | MS.gene25704 | PPI |
| MS.gene25703 | MS.gene77468 | PPI |
| MS.gene25703 | MS.gene71531 | PPI |
| MS.gene25703 | MS.gene044323 | PPI |
| MS.gene25703 | MS.gene71284 | PPI |
| MS.gene25703 | MS.gene044376 | PPI |
| MS.gene25703 | MS.gene70430 | PPI |
| MS.gene70443 | MS.gene25703 | PPI |
| MS.gene25703 | MS.gene045214 | PPI |
| MS.gene044363 | MS.gene25703 | PPI |
| MS.gene25703 | MS.gene77466 | PPI |
| MS.gene25703 | MS.gene044361 | PPI |
| MS.gene25703 | MS.gene71282 | PPI |
| MS.gene70418 | MS.gene25703 | PPI |
| MS.gene25703 | MS.gene71524 | PPI |
| MS.gene25703 | MS.gene71528 | PPI |
| MS.gene25703 | MS.gene70424 | PPI |
| MS.gene25703 | MS.gene77464 | PPI |
| MS.gene25703 | MS.gene044326 | PPI |
| MS.gene77472 | MS.gene25703 | PPI |
| MS.gene25703 | MS.gene79115 | PPI |
| MS.gene25703 | MS.gene70427 | PPI |
| MS.gene25703 | MS.gene049263 | PPI |
| MS.gene25703 | MS.gene044370 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25703.t1 | MTR_3g082420 | 79.137 | 139 | 27 | 2 | 1 | 138 | 85 | 222 | 5.58e-74 | 220 |
| MS.gene25703.t1 | MTR_3g082340 | 79.137 | 139 | 27 | 2 | 1 | 138 | 86 | 223 | 1.03e-73 | 219 |
| MS.gene25703.t1 | MTR_3g080680 | 49.645 | 141 | 63 | 4 | 1 | 138 | 64 | 199 | 1.22e-36 | 124 |
| MS.gene25703.t1 | MTR_3g083050 | 43.382 | 136 | 61 | 7 | 5 | 137 | 84 | 206 | 4.63e-19 | 79.7 |
| MS.gene25703.t1 | MTR_3g083030 | 43.382 | 136 | 61 | 7 | 5 | 137 | 107 | 229 | 6.84e-19 | 79.7 |
| MS.gene25703.t1 | MTR_8g461300 | 38.889 | 144 | 79 | 3 | 1 | 137 | 57 | 198 | 9.08e-19 | 78.6 |
| MS.gene25703.t1 | MTR_4g055800 | 40.650 | 123 | 58 | 3 | 1 | 121 | 164 | 273 | 2.89e-16 | 73.6 |
| MS.gene25703.t1 | MTR_3g083080 | 35.338 | 133 | 77 | 4 | 5 | 137 | 82 | 205 | 1.98e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTTTCCAGGCTCAAGTA+TGG | 0.302357 | 3.3:+69325130 | MS.gene25703:CDS |
| CCCTGCTTTAACAACTTTCC+AGG | 0.345313 | 3.3:+69325119 | MS.gene25703:CDS |
| CCGCCACATAACAGTGTTCT+TGG | 0.391644 | 3.3:-69324777 | None:intergenic |
| TGAACTCTCCCTGCTTGCAT+TGG | 0.445183 | 3.3:+69324845 | MS.gene25703:CDS |
| GCCTGGAAAGTTGTTAAAGC+AGG | 0.451262 | 3.3:-69325120 | None:intergenic |
| ATCGGTTATGAAGAGGGGTT+GGG | 0.467286 | 3.3:-69324802 | None:intergenic |
| TGTAATATTCCAATGCAAGC+AGG | 0.487476 | 3.3:-69324854 | None:intergenic |
| CACCGTCCCATACTTGAGCC+TGG | 0.497315 | 3.3:-69325137 | None:intergenic |
| TAACAGTGTTCTTGGCGGAC+GGG | 0.498416 | 3.3:-69324769 | None:intergenic |
| ACTTGATGAAACTCAAATGT+TGG | 0.500719 | 3.3:-69325000 | None:intergenic |
| CATCGGTTATGAAGAGGGGT+TGG | 0.502034 | 3.3:-69324803 | None:intergenic |
| AACAGTGTTCTTGGCGGACG+GGG | 0.511969 | 3.3:-69324768 | None:intergenic |
| ATCAGCAATACCAACATCAT+AGG | 0.513179 | 3.3:-69325034 | None:intergenic |
| ATCTTCATCGGTTATGAAGA+GGG | 0.524106 | 3.3:-69324808 | None:intergenic |
| AACTTTCCAGGCTCAAGTAT+GGG | 0.533504 | 3.3:+69325131 | MS.gene25703:CDS |
| CAGGGAGAGTTCATCAAGAG+TGG | 0.544597 | 3.3:-69324835 | None:intergenic |
| AGGGAGAGTTCATCAAGAGT+GGG | 0.550261 | 3.3:-69324834 | None:intergenic |
| AAGAGTGGGGCGATCTTCAT+CGG | 0.559401 | 3.3:-69324820 | None:intergenic |
| ATAACAGTGTTCTTGGCGGA+CGG | 0.562366 | 3.3:-69324770 | None:intergenic |
| TATGATGTTGGTATTGCTGA+TGG | 0.567223 | 3.3:+69325036 | MS.gene25703:CDS |
| CTTGCATTGGAATATTACAA+TGG | 0.578867 | 3.3:+69324858 | MS.gene25703:CDS |
| GTAATATTCCAATGCAAGCA+GGG | 0.591856 | 3.3:-69324853 | None:intergenic |
| CCACATAACAGTGTTCTTGG+CGG | 0.600956 | 3.3:-69324774 | None:intergenic |
| CCTGGAAAGTTGTTAAAGCA+GGG | 0.605506 | 3.3:-69325119 | None:intergenic |
| AGTTGTTAAAGCAGGGCTGT+CGG | 0.619352 | 3.3:-69325112 | None:intergenic |
| CCGCCAAGAACACTGTTATG+TGG | 0.619826 | 3.3:+69324774 | MS.gene25703:CDS |
| GATCTTCATCGGTTATGAAG+AGG | 0.624382 | 3.3:-69324809 | None:intergenic |
| GTCAAGTGTACCTATGATGT+TGG | 0.629074 | 3.3:+69325024 | MS.gene25703:CDS |
| TCTTCATCGGTTATGAAGAG+GGG | 0.634517 | 3.3:-69324807 | None:intergenic |
| TTCCAGGCTCAAGTATGGGA+CGG | 0.638739 | 3.3:+69325135 | MS.gene25703:CDS |
| ACAGTGTTCTTGGCGGACGG+GGG | 0.652393 | 3.3:-69324767 | None:intergenic |
| TAAACTCATGAAGAAACTAG+AGG | 0.657097 | 3.3:+69324589 | MS.gene25703:CDS |
| GGGAGAGTTCATCAAGAGTG+GGG | 0.657488 | 3.3:-69324833 | None:intergenic |
| CCAAGAACACTGTTATGTGG+CGG | 0.693885 | 3.3:+69324777 | MS.gene25703:CDS |
| GAGCATCTAGAGCGTTCTCG+CGG | 0.715724 | 3.3:+69324623 | MS.gene25703:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCTTCTTAATTAATTATT+AGG | + | chr3.3:69324975-69324994 | MS.gene25703:intron | 15.0% |
| !! | CAATGGAAAAAATAATAATC+AGG | + | chr3.3:69324875-69324894 | MS.gene25703:CDS | 20.0% |
| !!! | TAATTAATTTTGATTCATCC+TGG | + | chr3.3:69324716-69324735 | MS.gene25703:intron | 20.0% |
| ACTTGATGAAACTCAAATGT+TGG | - | chr3.3:69325003-69325022 | None:intergenic | 30.0% | |
| CTTGCATTGGAATATTACAA+TGG | + | chr3.3:69324858-69324877 | MS.gene25703:CDS | 30.0% | |
| TAAACTCATGAAGAAACTAG+AGG | + | chr3.3:69324589-69324608 | MS.gene25703:CDS | 30.0% | |
| !! | GAAGTGTCTTCTTTTGATAT+TGG | - | chr3.3:69325093-69325112 | None:intergenic | 30.0% |
| !!! | TGATATTGGTTTTGCTTGAA+AGG | - | chr3.3:69325079-69325098 | None:intergenic | 30.0% |
| ATCAGCAATACCAACATCAT+AGG | - | chr3.3:69325037-69325056 | None:intergenic | 35.0% | |
| GTAATATTCCAATGCAAGCA+GGG | - | chr3.3:69324856-69324875 | None:intergenic | 35.0% | |
| TATGATGTTGGTATTGCTGA+TGG | + | chr3.3:69325036-69325055 | MS.gene25703:CDS | 35.0% | |
| TGTAATATTCCAATGCAAGC+AGG | - | chr3.3:69324857-69324876 | None:intergenic | 35.0% | |
| ! | ATCTTCATCGGTTATGAAGA+GGG | - | chr3.3:69324811-69324830 | None:intergenic | 35.0% |
| AACTTTCCAGGCTCAAGTAT+GGG | + | chr3.3:69325131-69325150 | MS.gene25703:CDS | 40.0% | |
| CCTGGAAAGTTGTTAAAGCA+GGG | - | chr3.3:69325122-69325141 | None:intergenic | 40.0% | |
| GTCAAGTGTACCTATGATGT+TGG | + | chr3.3:69325024-69325043 | MS.gene25703:CDS | 40.0% | |
| ! | GATCTTCATCGGTTATGAAG+AGG | - | chr3.3:69324812-69324831 | None:intergenic | 40.0% |
| ! | TCTTCATCGGTTATGAAGAG+GGG | - | chr3.3:69324810-69324829 | None:intergenic | 40.0% |
| ACACCTGTATTGTGTTGTCC+AGG | - | chr3.3:69324737-69324756 | None:intergenic | 45.0% | |
| AGGGAGAGTTCATCAAGAGT+GGG | - | chr3.3:69324837-69324856 | None:intergenic | 45.0% | |
| AGTTGTTAAAGCAGGGCTGT+CGG | - | chr3.3:69325115-69325134 | None:intergenic | 45.0% | |
| ATAACAGTGTTCTTGGCGGA+CGG | - | chr3.3:69324773-69324792 | None:intergenic | 45.0% | |
| ATAGACGCGTACGTACACTT+AGG | - | chr3.3:69324649-69324668 | None:intergenic | 45.0% | |
| ATCGGTTATGAAGAGGGGTT+GGG | - | chr3.3:69324805-69324824 | None:intergenic | 45.0% | |
| CAACTTTCCAGGCTCAAGTA+TGG | + | chr3.3:69325130-69325149 | MS.gene25703:CDS | 45.0% | |
| CATCCTGGACAACACAATAC+AGG | + | chr3.3:69324731-69324750 | MS.gene25703:intron | 45.0% | |
| CCAAGAACACTGTTATGTGG+CGG | + | chr3.3:69324777-69324796 | MS.gene25703:CDS | 45.0% | |
| CCACATAACAGTGTTCTTGG+CGG | - | chr3.3:69324777-69324796 | None:intergenic | 45.0% | |
| CCCTGCTTTAACAACTTTCC+AGG | + | chr3.3:69325119-69325138 | MS.gene25703:CDS | 45.0% | |
| GCCTGGAAAGTTGTTAAAGC+AGG | - | chr3.3:69325123-69325142 | None:intergenic | 45.0% | |
| AAGAGTGGGGCGATCTTCAT+CGG | - | chr3.3:69324823-69324842 | None:intergenic | 50.0% | |
| CAGGGAGAGTTCATCAAGAG+TGG | - | chr3.3:69324838-69324857 | None:intergenic | 50.0% | |
| CCGCCAAGAACACTGTTATG+TGG | + | chr3.3:69324774-69324793 | MS.gene25703:CDS | 50.0% | |
| CCGCCACATAACAGTGTTCT+TGG | - | chr3.3:69324780-69324799 | None:intergenic | 50.0% | |
| GGGAGAGTTCATCAAGAGTG+GGG | - | chr3.3:69324836-69324855 | None:intergenic | 50.0% | |
| TGAACTCTCCCTGCTTGCAT+TGG | + | chr3.3:69324845-69324864 | MS.gene25703:CDS | 50.0% | |
| TTCCAGGCTCAAGTATGGGA+CGG | + | chr3.3:69325135-69325154 | MS.gene25703:CDS | 50.0% | |
| ! | CATCGGTTATGAAGAGGGGT+TGG | - | chr3.3:69324806-69324825 | None:intergenic | 50.0% |
| ! | TAACAGTGTTCTTGGCGGAC+GGG | - | chr3.3:69324772-69324791 | None:intergenic | 50.0% |
| ! | AACAGTGTTCTTGGCGGACG+GGG | - | chr3.3:69324771-69324790 | None:intergenic | 55.0% |
| ! | GAGCATCTAGAGCGTTCTCG+CGG | + | chr3.3:69324623-69324642 | MS.gene25703:CDS | 55.0% |
| CACCGTCCCATACTTGAGCC+TGG | - | chr3.3:69325140-69325159 | None:intergenic | 60.0% | |
| ! | ACAGTGTTCTTGGCGGACGG+GGG | - | chr3.3:69324770-69324789 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 69324587 | 69325203 | 69324587 | ID=MS.gene25703 |
| chr3.3 | mRNA | 69324587 | 69325203 | 69324587 | ID=MS.gene25703.t1;Parent=MS.gene25703 |
| chr3.3 | exon | 69324587 | 69324652 | 69324587 | ID=MS.gene25703.t1.exon1;Parent=MS.gene25703.t1 |
| chr3.3 | CDS | 69324587 | 69324652 | 69324587 | ID=cds.MS.gene25703.t1;Parent=MS.gene25703.t1 |
| chr3.3 | exon | 69324753 | 69324896 | 69324753 | ID=MS.gene25703.t1.exon2;Parent=MS.gene25703.t1 |
| chr3.3 | CDS | 69324753 | 69324896 | 69324753 | ID=cds.MS.gene25703.t1;Parent=MS.gene25703.t1 |
| chr3.3 | exon | 69324997 | 69325203 | 69324997 | ID=MS.gene25703.t1.exon3;Parent=MS.gene25703.t1 |
| chr3.3 | CDS | 69324997 | 69325203 | 69324997 | ID=cds.MS.gene25703.t1;Parent=MS.gene25703.t1 |
| Gene Sequence |
| Protein sequence |
