Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25749.t1 | XP_003601436.2 | 66.8 | 193 | 52 | 4 | 16 | 197 | 7 | 198 | 3.80E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25749.t1 | G7J7C7 | 66.8 | 193 | 52 | 4 | 16 | 197 | 7 | 198 | 2.8e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77468 | MS.gene25749 | PPI |
| MS.gene25749 | MS.gene044323 | PPI |
| MS.gene045214 | MS.gene25749 | PPI |
| MS.gene044361 | MS.gene25749 | PPI |
| MS.gene77466 | MS.gene25749 | PPI |
| MS.gene044370 | MS.gene25749 | PPI |
| MS.gene70476 | MS.gene25749 | PPI |
| MS.gene25704 | MS.gene25749 | PPI |
| MS.gene70443 | MS.gene25749 | PPI |
| MS.gene25749 | MS.gene71524 | PPI |
| MS.gene79115 | MS.gene25749 | PPI |
| MS.gene71531 | MS.gene25749 | PPI |
| MS.gene70430 | MS.gene25749 | PPI |
| MS.gene71282 | MS.gene25749 | PPI |
| MS.gene70424 | MS.gene25749 | PPI |
| MS.gene71528 | MS.gene25749 | PPI |
| MS.gene25749 | MS.gene044326 | PPI |
| MS.gene044376 | MS.gene25749 | PPI |
| MS.gene044363 | MS.gene25749 | PPI |
| MS.gene25749 | MS.gene70427 | PPI |
| MS.gene77464 | MS.gene25749 | PPI |
| MS.gene71284 | MS.gene25749 | PPI |
| MS.gene77472 | MS.gene25749 | PPI |
| MS.gene70418 | MS.gene25749 | PPI |
| MS.gene25749 | MS.gene049263 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25749.t1 | MTR_3g080680 | 66.667 | 195 | 53 | 4 | 14 | 197 | 5 | 198 | 2.48e-83 | 246 |
| MS.gene25749.t1 | MTR_3g082420 | 39.819 | 221 | 109 | 6 | 1 | 197 | 1 | 221 | 1.08e-37 | 130 |
| MS.gene25749.t1 | MTR_3g082340 | 40.541 | 222 | 107 | 8 | 1 | 197 | 1 | 222 | 2.62e-37 | 129 |
| MS.gene25749.t1 | MTR_3g083080 | 41.709 | 199 | 104 | 6 | 7 | 198 | 13 | 206 | 8.15e-37 | 127 |
| MS.gene25749.t1 | MTR_8g461300 | 45.783 | 166 | 76 | 5 | 44 | 197 | 35 | 198 | 4.03e-36 | 125 |
| MS.gene25749.t1 | MTR_4g055800 | 42.361 | 144 | 66 | 5 | 47 | 184 | 144 | 276 | 6.86e-27 | 104 |
| MS.gene25749.t1 | MTR_3g083050 | 38.967 | 213 | 107 | 9 | 1 | 197 | 1 | 206 | 6.82e-26 | 99.8 |
| MS.gene25749.t1 | MTR_3g083030 | 40.331 | 181 | 95 | 7 | 23 | 197 | 56 | 229 | 3.98e-25 | 98.2 |
| MS.gene25749.t1 | MTR_3g083150 | 36.406 | 217 | 112 | 10 | 1 | 197 | 1 | 211 | 1.48e-18 | 80.5 |
| MS.gene25749.t1 | MTR_3g086030 | 39.583 | 96 | 53 | 2 | 33 | 123 | 14 | 109 | 3.05e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 56 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATCTCAGGTGGTTTAA+TGG | 0.243396 | 3.2:+71240753 | None:intergenic |
| GCCTGGAAAGTTGTTATATC+AGG | 0.246103 | 3.2:+71240823 | None:intergenic |
| TTACCACGTTTGCCGTAATA+AGG | 0.262079 | 3.2:+71241373 | None:intergenic |
| AATAGATTCTACCATTAATA+AGG | 0.262811 | 3.2:+71240890 | None:intergenic |
| TGTACCCATGCCCTTATTAA+TGG | 0.282280 | 3.2:-71240901 | MS.gene25749:CDS |
| CCGAGAGAGGGATCGGGATC+GGG | 0.379715 | 3.2:-71241460 | MS.gene25749:CDS |
| CCGCCAAGATCAATGATAAC+TGG | 0.380453 | 3.2:-71241157 | MS.gene25749:CDS |
| GGCTCAAATTCAAATCTATA+AGG | 0.386127 | 3.2:-71241314 | MS.gene25749:CDS |
| GAGGATTTCCAGGGCAGGTT+TGG | 0.402561 | 3.2:+71241510 | None:intergenic |
| CCCTGATATAACAACTTTCC+AGG | 0.412336 | 3.2:-71240824 | MS.gene25749:CDS |
| AGTATAAGAATGAGGCATAT+AGG | 0.413071 | 3.2:-71241345 | MS.gene25749:CDS |
| CCATGCAAATAACAATAATC+AGG | 0.424980 | 3.2:-71241056 | MS.gene25749:intron |
| GATTCCGAATTTCCTTATTA+CGG | 0.437307 | 3.2:-71241385 | MS.gene25749:CDS |
| GCGCCAGTTATCATTGATCT+TGG | 0.437849 | 3.2:+71241154 | None:intergenic |
| GCCGAGAGAGGGATCGGGAT+CGG | 0.440845 | 3.2:-71241461 | MS.gene25749:CDS |
| TTTGCCGTAATAAGGAAATT+CGG | 0.455567 | 3.2:+71241381 | None:intergenic |
| GTTATCATTGATCTTGGCGG+AGG | 0.467990 | 3.2:+71241160 | None:intergenic |
| ATCACTCTCCAAGCTTGCAT+TGG | 0.473320 | 3.2:-71241086 | MS.gene25749:CDS |
| ATAGATTCTACCATTAATAA+GGG | 0.476198 | 3.2:+71240891 | None:intergenic |
| AAGCGGCCGAGAGAGGGATC+GGG | 0.483001 | 3.2:-71241466 | MS.gene25749:CDS |
| GAGAGTAGGGCGAACATCAT+CGG | 0.484219 | 3.2:+71241111 | None:intergenic |
| CCTGATTATTGTTATTTGCA+TGG | 0.484343 | 3.2:+71241056 | None:intergenic |
| AGGCCATGAAACACAAAAGT+TGG | 0.488464 | 3.2:+71240931 | None:intergenic |
| GAAGCGGCCGAGAGAGGGAT+CGG | 0.489234 | 3.2:-71241467 | MS.gene25749:CDS |
| GTGGCACACTCTCTGATCTC+AGG | 0.491079 | 3.2:+71240742 | None:intergenic |
| TACTTTACAAGATCTTCAAC+GGG | 0.491367 | 3.2:+71241424 | None:intergenic |
| TTGGAGAGTGATTCGAGAGT+AGG | 0.496843 | 3.2:+71241097 | None:intergenic |
| TGGAGAGTGATTCGAGAGTA+GGG | 0.500523 | 3.2:+71241098 | None:intergenic |
| CCCGATCCCGATCCCTCTCT+CGG | 0.502758 | 3.2:+71241460 | None:intergenic |
| GTAATAGTCCAATGCAAGCT+TGG | 0.503651 | 3.2:+71241078 | None:intergenic |
| TTCTACCATTAATAAGGGCA+TGG | 0.508172 | 3.2:+71240896 | None:intergenic |
| GTTGGATACCAAACCTGCCC+TGG | 0.512189 | 3.2:-71241518 | MS.gene25749:CDS |
| CCAGTTATCATTGATCTTGG+CGG | 0.521158 | 3.2:+71241157 | None:intergenic |
| GGATCGAAGCGGCCGAGAGA+GGG | 0.529420 | 3.2:-71241472 | MS.gene25749:CDS |
| GTACTTTACAAGATCTTCAA+CGG | 0.537524 | 3.2:+71241423 | None:intergenic |
| TTATCATTGATCTTGGCGGA+GGG | 0.559160 | 3.2:+71241161 | None:intergenic |
| TTTCCTTATTACGGCAAACG+TGG | 0.561333 | 3.2:-71241376 | MS.gene25749:CDS |
| TTGCAGAATGTCGATGGAGT+TGG | 0.564508 | 3.2:-71241536 | None:intergenic |
| TCATCGAGGATTTCCAGGGC+AGG | 0.571513 | 3.2:+71241505 | None:intergenic |
| ATGGCACACTCATTGACCAC+AGG | 0.572965 | 3.2:+71240772 | None:intergenic |
| CCTGGAAAGTTGTTATATCA+GGG | 0.574585 | 3.2:+71240824 | None:intergenic |
| TTCGTGTTGCAGAATGTCGA+TGG | 0.578989 | 3.2:-71241542 | None:intergenic |
| GCTCTGAGCATACTTCAGCC+TGG | 0.579022 | 3.2:+71240806 | None:intergenic |
| GAACATCATCGGATATGTGA+AGG | 0.588800 | 3.2:+71241122 | None:intergenic |
| AGTTGTTATATCAGGGCTGT+CGG | 0.591200 | 3.2:+71240831 | None:intergenic |
| AACATCATCGGATATGTGAA+GGG | 0.592088 | 3.2:+71241123 | None:intergenic |
| TATCATTGATCTTGGCGGAG+GGG | 0.592257 | 3.2:+71241162 | None:intergenic |
| TCTACCATTAATAAGGGCAT+GGG | 0.593250 | 3.2:+71240897 | None:intergenic |
| GTTATATCAGGGCTGTCGGA+AGG | 0.604717 | 3.2:+71240835 | None:intergenic |
| AGGATCGAAGCGGCCGAGAG+AGG | 0.618028 | 3.2:-71241473 | MS.gene25749:CDS |
| GCACACTCTCTGATCTCAGG+TGG | 0.621362 | 3.2:+71240745 | None:intergenic |
| GGGCATGGGTACACTTGACA+AGG | 0.652935 | 3.2:+71240911 | None:intergenic |
| TGAGGCATATAGGAAGCTTG+AGG | 0.665865 | 3.2:-71241335 | MS.gene25749:CDS |
| ACATCATCGGATATGTGAAG+GGG | 0.669393 | 3.2:+71241124 | None:intergenic |
| AGAGCGAGATAAACAACCTG+TGG | 0.683787 | 3.2:-71240788 | MS.gene25749:CDS |
| TGGAGTGTATTAGATTGTAG+TGG | 0.692541 | 3.2:+71240723 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACAAAATTGAAATAGA+TGG | + | chr3.2:71241018-71241037 | None:intergenic | 15.0% |
| !! | AATAGATTCTACCATTAATA+AGG | + | chr3.2:71241374-71241393 | None:intergenic | 20.0% |
| !! | AGAAGAACAATTAATTAGTT+TGG | + | chr3.2:71241295-71241314 | None:intergenic | 20.0% |
| !! | ATAGATTCTACCATTAATAA+GGG | + | chr3.2:71241373-71241392 | None:intergenic | 20.0% |
| !! | TGTTCTTCTTCATTATATTA+GGG | - | chr3.2:71241306-71241325 | MS.gene25749:CDS | 20.0% |
| !! | TTGTTCTTCTTCATTATATT+AGG | - | chr3.2:71241305-71241324 | MS.gene25749:CDS | 20.0% |
| !!! | TAATTTTGAGTATAAGAATG+AGG | - | chr3.2:71240908-71240927 | MS.gene25749:CDS | 20.0% |
| ! | ATAGATAGATGTTTACACTT+AGG | + | chr3.2:71240986-71241005 | None:intergenic | 25.0% |
| AGTATAAGAATGAGGCATAT+AGG | - | chr3.2:71240916-71240935 | MS.gene25749:CDS | 30.0% | |
| CCATGCAAATAACAATAATC+AGG | - | chr3.2:71241205-71241224 | MS.gene25749:intron | 30.0% | |
| GATTCCGAATTTCCTTATTA+CGG | - | chr3.2:71240876-71240895 | MS.gene25749:CDS | 30.0% | |
| GGCTCAAATTCAAATCTATA+AGG | - | chr3.2:71240947-71240966 | MS.gene25749:intron | 30.0% | |
| GTACTTTACAAGATCTTCAA+CGG | + | chr3.2:71240841-71240860 | None:intergenic | 30.0% | |
| TACTTTACAAGATCTTCAAC+GGG | + | chr3.2:71240840-71240859 | None:intergenic | 30.0% | |
| TTTGCCGTAATAAGGAAATT+CGG | + | chr3.2:71240883-71240902 | None:intergenic | 30.0% | |
| !! | CCTGATTATTGTTATTTGCA+TGG | + | chr3.2:71241208-71241227 | None:intergenic | 30.0% |
| AAAAAGAGAAAGGATCGAAG+CGG | - | chr3.2:71240778-71240797 | MS.gene25749:CDS | 35.0% | |
| AACATCATCGGATATGTGAA+GGG | + | chr3.2:71241141-71241160 | None:intergenic | 35.0% | |
| ATCCTCGATGAAAAAGAGAA+AGG | - | chr3.2:71240768-71240787 | MS.gene25749:CDS | 35.0% | |
| CCTGGAAAGTTGTTATATCA+GGG | + | chr3.2:71241440-71241459 | None:intergenic | 35.0% | |
| TTCTACCATTAATAAGGGCA+TGG | + | chr3.2:71241368-71241387 | None:intergenic | 35.0% | |
| ! | TCTACCATTAATAAGGGCAT+GGG | + | chr3.2:71241367-71241386 | None:intergenic | 35.0% |
| ! | TTTTTCATCGAGGATTTCCA+GGG | + | chr3.2:71240763-71240782 | None:intergenic | 35.0% |
| !! | ATCCTTTCTCTTTTTCATCG+AGG | + | chr3.2:71240773-71240792 | None:intergenic | 35.0% |
| ACATCATCGGATATGTGAAG+GGG | + | chr3.2:71241140-71241159 | None:intergenic | 40.0% | |
| AGGCCATGAAACACAAAAGT+TGG | + | chr3.2:71241333-71241352 | None:intergenic | 40.0% | |
| AGTTGTTATATCAGGGCTGT+CGG | + | chr3.2:71241433-71241452 | None:intergenic | 40.0% | |
| CCAGTTATCATTGATCTTGG+CGG | + | chr3.2:71241107-71241126 | None:intergenic | 40.0% | |
| CCCTGATATAACAACTTTCC+AGG | - | chr3.2:71241437-71241456 | MS.gene25749:CDS | 40.0% | |
| GAACATCATCGGATATGTGA+AGG | + | chr3.2:71241142-71241161 | None:intergenic | 40.0% | |
| GCCTGGAAAGTTGTTATATC+AGG | + | chr3.2:71241441-71241460 | None:intergenic | 40.0% | |
| GTAATAGTCCAATGCAAGCT+TGG | + | chr3.2:71241186-71241205 | None:intergenic | 40.0% | |
| TCTGATCTCAGGTGGTTTAA+TGG | + | chr3.2:71241511-71241530 | None:intergenic | 40.0% | |
| TGTACCCATGCCCTTATTAA+TGG | - | chr3.2:71241360-71241379 | MS.gene25749:CDS | 40.0% | |
| TTACCACGTTTGCCGTAATA+AGG | + | chr3.2:71240891-71240910 | None:intergenic | 40.0% | |
| TTATCATTGATCTTGGCGGA+GGG | + | chr3.2:71241103-71241122 | None:intergenic | 40.0% | |
| TTTCCTTATTACGGCAAACG+TGG | - | chr3.2:71240885-71240904 | MS.gene25749:CDS | 40.0% | |
| ! | GGTCCAACTTTTGTGTTTCA+TGG | - | chr3.2:71241327-71241346 | MS.gene25749:CDS | 40.0% |
| !! | AGGATCTTCTTCTGCTTTTG+CGG | + | chr3.2:71241409-71241428 | None:intergenic | 40.0% |
| !! | CTTTTTCATCGAGGATTTCC+AGG | + | chr3.2:71240764-71240783 | None:intergenic | 40.0% |
| !! | TTCTTCTGCTTTTGCGGTAA+AGG | + | chr3.2:71241403-71241422 | None:intergenic | 40.0% |
| AGAGCGAGATAAACAACCTG+TGG | - | chr3.2:71241473-71241492 | MS.gene25749:CDS | 45.0% | |
| ATCACTCTCCAAGCTTGCAT+TGG | - | chr3.2:71241175-71241194 | MS.gene25749:CDS | 45.0% | |
| CCGCCAAGATCAATGATAAC+TGG | - | chr3.2:71241104-71241123 | MS.gene25749:CDS | 45.0% | |
| GCGCCAGTTATCATTGATCT+TGG | + | chr3.2:71241110-71241129 | None:intergenic | 45.0% | |
| GTTATCATTGATCTTGGCGG+AGG | + | chr3.2:71241104-71241123 | None:intergenic | 45.0% | |
| TATCATTGATCTTGGCGGAG+GGG | + | chr3.2:71241102-71241121 | None:intergenic | 45.0% | |
| TGAGGCATATAGGAAGCTTG+AGG | - | chr3.2:71240926-71240945 | MS.gene25749:CDS | 45.0% | |
| TGGAGAGTGATTCGAGAGTA+GGG | + | chr3.2:71241166-71241185 | None:intergenic | 45.0% | |
| TTGGAGAGTGATTCGAGAGT+AGG | + | chr3.2:71241167-71241186 | None:intergenic | 45.0% | |
| ATGGCACACTCATTGACCAC+AGG | + | chr3.2:71241492-71241511 | None:intergenic | 50.0% | |
| GAGAGTAGGGCGAACATCAT+CGG | + | chr3.2:71241153-71241172 | None:intergenic | 50.0% | |
| GTTATATCAGGGCTGTCGGA+AGG | + | chr3.2:71241429-71241448 | None:intergenic | 50.0% | |
| GAGGATTTCCAGGGCAGGTT+TGG | + | chr3.2:71240754-71240773 | None:intergenic | 55.0% | |
| GCACACTCTCTGATCTCAGG+TGG | + | chr3.2:71241519-71241538 | None:intergenic | 55.0% | |
| GGGCATGGGTACACTTGACA+AGG | + | chr3.2:71241353-71241372 | None:intergenic | 55.0% | |
| GTGGCACACTCTCTGATCTC+AGG | + | chr3.2:71241522-71241541 | None:intergenic | 55.0% | |
| GTTGGATACCAAACCTGCCC+TGG | - | chr3.2:71240743-71240762 | MS.gene25749:CDS | 55.0% | |
| TCATCGAGGATTTCCAGGGC+AGG | + | chr3.2:71240759-71240778 | None:intergenic | 55.0% | |
| ! | GCTCTGAGCATACTTCAGCC+TGG | + | chr3.2:71241458-71241477 | None:intergenic | 55.0% |
| AAGCGGCCGAGAGAGGGATC+GGG | - | chr3.2:71240795-71240814 | MS.gene25749:CDS | 65.0% | |
| AGGATCGAAGCGGCCGAGAG+AGG | - | chr3.2:71240788-71240807 | MS.gene25749:CDS | 65.0% | |
| CCCGATCCCGATCCCTCTCT+CGG | + | chr3.2:71240804-71240823 | None:intergenic | 65.0% | |
| CCGAGAGAGGGATCGGGATC+GGG | - | chr3.2:71240801-71240820 | MS.gene25749:CDS | 65.0% | |
| GAAGCGGCCGAGAGAGGGAT+CGG | - | chr3.2:71240794-71240813 | MS.gene25749:CDS | 65.0% | |
| GCCGAGAGAGGGATCGGGAT+CGG | - | chr3.2:71240800-71240819 | MS.gene25749:CDS | 65.0% | |
| GGATCGAAGCGGCCGAGAGA+GGG | - | chr3.2:71240789-71240808 | MS.gene25749:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 71240732 | 71241551 | 71240732 | ID=MS.gene25749 |
| chr3.2 | mRNA | 71240732 | 71241551 | 71240732 | ID=MS.gene25749.t1;Parent=MS.gene25749 |
| chr3.2 | exon | 71241294 | 71241551 | 71241294 | ID=MS.gene25749.t1.exon1;Parent=MS.gene25749.t1 |
| chr3.2 | CDS | 71241294 | 71241551 | 71241294 | ID=cds.MS.gene25749.t1;Parent=MS.gene25749.t1 |
| chr3.2 | exon | 71241057 | 71241200 | 71241057 | ID=MS.gene25749.t1.exon2;Parent=MS.gene25749.t1 |
| chr3.2 | CDS | 71241057 | 71241200 | 71241057 | ID=cds.MS.gene25749.t1;Parent=MS.gene25749.t1 |
| chr3.2 | exon | 71240732 | 71240956 | 71240732 | ID=MS.gene25749.t1.exon3;Parent=MS.gene25749.t1 |
| chr3.2 | CDS | 71240732 | 71240956 | 71240732 | ID=cds.MS.gene25749.t1;Parent=MS.gene25749.t1 |
| Gene Sequence |
| Protein sequence |
