AlfalfaGEDB | MS.gene25870

M. sativa cultivar XinJiangDaYe / MS.gene25870

KEGG: K00454
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0008150 GO:0008152 GO:0016491 GO:0016701 GO:0016702 GO:0043167 GO:0043169 GO:0046872 GO:0051213 GO:0055114
PFAM: PF00305.22 PF01477.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25870.t1	XP_003601325.2	96.4	841	29	1	1	841	63	902	0.00E+00	1650.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25870.t1	O24370	58.8	839	338	8	8	841	64	899	2.2e-295	1016.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25870.t1	G7J632	96.4	841	29	1	1	841	63	902	0.0e+00	1650.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049217	MS.gene25870	0.800923	1.15E-48	-1.69E-46
MS.gene049907	MS.gene25870	0.84022	9.92E-58	-1.69E-46
MS.gene050520	MS.gene25870	0.858134	1.02E-62	-1.69E-46
MS.gene051255	MS.gene25870	0.84346	1.38E-58	-1.69E-46
MS.gene051387	MS.gene25870	0.842929	1.91E-58	-1.69E-46
MS.gene053207	MS.gene25870	0.823471	1.36E-53	-1.69E-46
MS.gene053213	MS.gene25870	0.803411	3.53E-49	-1.69E-46
MS.gene053286	MS.gene25870	0.802453	5.57E-49	-1.69E-46
MS.gene053813	MS.gene25870	0.824538	7.65E-54	-1.69E-46
MS.gene054486	MS.gene25870	0.802232	6.19E-49	-1.69E-46
MS.gene054595	MS.gene25870	0.808793	2.60E-50	-1.69E-46
MS.gene055911	MS.gene25870	0.801778	7.67E-49	-1.69E-46
MS.gene05601	MS.gene25870	0.807899	4.03E-50	-1.69E-46
MS.gene057416	MS.gene25870	0.808535	2.95E-50	-1.69E-46
MS.gene058948	MS.gene25870	0.823464	1.37E-53	-1.69E-46
MS.gene05934	MS.gene25870	0.81451	1.48E-51	-1.69E-46
MS.gene059674	MS.gene25870	0.804348	2.26E-49	-1.69E-46
MS.gene059680	MS.gene25870	0.813567	2.40E-51	-1.69E-46
MS.gene059711	MS.gene25870	0.841773	3.88E-58	-1.69E-46
MS.gene060356	MS.gene25870	0.804185	2.44E-49	-1.69E-46
MS.gene060482	MS.gene25870	0.817574	3.07E-52	-1.69E-46
MS.gene060804	MS.gene25870	0.834445	2.98E-56	-1.69E-46
MS.gene060837	MS.gene25870	0.825665	4.14E-54	-1.69E-46
MS.gene061134	MS.gene25870	0.8034	3.55E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25870.t1	MTR_3g079450	96.671	841	27	1	1	841	63	902	0.0	1690
MS.gene25870.t1	MTR_3g479460	78.029	842	183	2	1	841	63	903	0.0	1404
MS.gene25870.t1	MTR_3g079420	80.283	847	155	4	6	841	20	865	0.0	1379
MS.gene25870.t1	MTR_3g079450	96.265	589	21	1	1	589	63	650	0.0	1172
MS.gene25870.t1	MTR_4g066170	61.051	837	310	8	16	841	75	906	0.0	1076
MS.gene25870.t1	MTR_8g020970	48.089	811	404	10	41	840	122	926	0.0	801
MS.gene25870.t1	MTR_8g080230	47.456	845	417	14	16	841	80	916	0.0	789
MS.gene25870.t1	MTR_1g104650	43.773	827	431	15	40	841	39	856	0.0	673
MS.gene25870.t1	MTR_4g088810	42.317	846	441	16	12	840	79	894	0.0	667
MS.gene25870.t1	MTR_1g083020	44.903	775	411	9	76	841	91	858	0.0	665
MS.gene25870.t1	MTR_2g099570	41.947	863	460	16	3	841	16	861	0.0	664
MS.gene25870.t1	MTR_8g018420	43.452	840	429	19	26	841	39	856	0.0	657
MS.gene25870.t1	MTR_8g018690	42.563	827	441	15	30	841	58	865	0.0	647
MS.gene25870.t1	MTR_2g099560	42.684	827	447	14	29	841	50	863	0.0	645
MS.gene25870.t1	MTR_8g018735	41.354	827	450	14	32	841	57	865	0.0	645
MS.gene25870.t1	MTR_8g018510	42.254	852	457	18	10	841	33	869	0.0	643
MS.gene25870.t1	MTR_8g018570	42.428	832	448	16	27	841	53	870	0.0	642
MS.gene25870.t1	MTR_8g018730	41.437	835	453	14	28	841	45	864	0.0	642
MS.gene25870.t1	MTR_7g113410	42.597	824	446	14	37	841	52	867	0.0	639
MS.gene25870.t1	MTR_8g020990	42.289	830	450	15	27	841	53	868	0.0	636
MS.gene25870.t1	MTR_8g018430	41.599	863	468	17	2	841	12	861	0.0	635
MS.gene25870.t1	MTR_8g018520	44.114	773	409	14	79	841	109	868	0.0	635
MS.gene25870.t1	MTR_8g018730	41.106	832	430	14	28	841	45	834	0.0	625
MS.gene25870.t1	MTR_5g024020	40.071	846	470	15	16	841	39	867	0.0	624
MS.gene25870.t1	MTR_8g018550	41.570	866	466	16	3	841	16	868	0.0	620
MS.gene25870.t1	MTR_8g018690	41.354	827	431	16	30	841	58	845	0.0	617
MS.gene25870.t1	MTR_8g018450	40.070	856	460	15	1	841	11	828	0.0	615
MS.gene25870.t1	MTR_3g081130	40.072	836	463	15	28	841	39	858	0.0	610
MS.gene25870.t1	MTR_3g081130	41.548	775	432	12	75	841	25	786	0.0	603
MS.gene25870.t1	MTR_5g024020	38.416	846	448	15	16	841	39	831	0.0	585
MS.gene25870.t1	MTR_8g018590	41.294	804	424	18	56	841	50	823	0.0	577
MS.gene25870.t1	MTR_8g018650	42.084	758	389	10	86	841	98	807	0.0	552
MS.gene25870.t1	MTR_8g018620	41.524	761	389	11	86	841	98	807	0.0	549
MS.gene25870.t1	MTR_8g011340	38.076	759	443	14	52	796	77	822	0.0	542
MS.gene25870.t1	MTR_8g020970	46.953	558	284	7	41	589	122	676	3.18e-178	529
MS.gene25870.t1	MTR_8g018730	42.321	586	318	9	264	841	11	584	1.15e-160	481
MS.gene25870.t1	MTR_8g018735	40.559	572	307	13	35	589	60	615	1.47e-134	415
MS.gene25870.t1	MTR_6g452500	35.940	601	361	13	232	824	21	605	2.50e-125	390
MS.gene25870.t1	MTR_8g021020	42.925	424	236	4	421	841	11	431	1.73e-112	350
MS.gene25870.t1	MTR_8g463810	58.190	232	33	2	394	625	190	357	3.80e-77	256
MS.gene25870.t1	MTR_7g012030	73.109	119	32	0	491	609	85	203	2.12e-59	201
MS.gene25870.t1	MTR_6g046330	66.154	130	33	1	491	609	75	204	1.14e-53	189
MS.gene25870.t1	MTR_2g067130	76.471	51	12	0	4	54	2	52	2.19e-18	83.6
MS.gene25870.t1	MTR_4g088805	47.253	91	47	1	751	840	1	91	1.33e-16	76.3
MS.gene25870.t1	MTR_5g052190	73.469	49	7	1	228	276	45	87	5.70e-12	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MS.gene25870.t1	AT3G45140	53.837	808	357	7	38	841	101	896	914
MS.gene25870.t1	AT3G45140	55.585	761	327	6	85	841	1	754	894
MS.gene25870.t1	AT1G72520	47.749	844	414	15	16	841	92	926	805
MS.gene25870.t1	AT1G17420	46.675	842	425	11	16	841	86	919	789
MS.gene25870.t1	AT1G67560	46.420	810	416	12	43	841	115	917	746
MS.gene25870.t1	AT1G55020	46.294	823	412	13	37	841	49	859	707
MS.gene25870.t1	AT3G22400	43.075	852	449	15	17	841	44	886	677

Find 164 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCATTCCAAAGTTCTTTA+AGG	0.084556	3.2:+69825017	MS.gene25870:CDS
CTAGCAATTGTTGGTCTATT+AGG	0.092716	3.2:-69826837	None:intergenic
CATGTTCTTGTTCATGATTC+TGG	0.145766	3.2:+69825848	MS.gene25870:CDS
CCAATGACGGTCTTCTAATA+TGG	0.163070	3.2:+69826556	MS.gene25870:CDS
TTTGAGTTACCAGCAAATTT+TGG	0.176746	3.2:+69822188	MS.gene25870:CDS
AACTTACTCTTGTATGGTTC+CGG	0.228161	3.2:-69825078	None:intergenic
TTAACCAATTGGATGCTATA+TGG	0.237885	3.2:-69825266	None:intergenic
GTGGCCTTTGAAAAGTAAAT+TGG	0.239348	3.2:+69825398	MS.gene25870:CDS
ATCAGTGACTATTGCGTCTA+AGG	0.241076	3.2:-69824893	None:intergenic
TATCAAAGCGCACGATATTT+TGG	0.241301	3.2:-69825053	None:intergenic
TGGAAATGTGGGTGCTGTTT+TGG	0.252907	3.2:+69822208	MS.gene25870:CDS
TGGTAACAAACACTTAATTT+TGG	0.253312	3.2:+69820178	MS.gene25870:CDS
TGTACCAAACTAGGTTTAAA+TGG	0.259190	3.2:-69827240	None:intergenic
CTGTGTGTTGCAATTATAAT+AGG	0.269350	3.2:-69826002	None:intergenic
ATGAAGAAAACTCTCTTTGT+AGG	0.284450	3.2:-69822344	None:intergenic
CCAACCAGAGCAAACACTTT+TGG	0.289754	3.2:+69826914	MS.gene25870:CDS
TAGCAATTGTTGGTCTATTA+GGG	0.301680	3.2:-69826836	None:intergenic
GTGACTCCAGGCCCTGAAAA+TGG	0.302315	3.2:-69827191	None:intergenic
TCTTTGGCAATTTGATTTGC+AGG	0.304593	3.2:+69826196	MS.gene25870:CDS
CTAAAGAATATAGTCCTTGA+TGG	0.311025	3.2:+69822260	MS.gene25870:CDS
GAGTCATTGGATTCAAGATT+TGG	0.317623	3.2:-69824979	None:intergenic
ATTCTGATTCCACCAATATC+TGG	0.318341	3.2:+69825804	MS.gene25870:CDS
TTTACACAATATGCTTATAT+AGG	0.319058	3.2:+69826807	MS.gene25870:CDS
ATTCTGGTGGACCATAGATT+TGG	0.321973	3.2:-69825425	None:intergenic
ACCTCAAAAGACCTGTTCTT+GGG	0.332457	3.2:+69824520	MS.gene25870:CDS
TTCACTCCATGTTGTGCATT+AGG	0.341211	3.2:-69826507	None:intergenic
GTGCGACACCGTCTAGGATA+TGG	0.348649	3.2:-69824558	None:intergenic
TCTCTAGTAATTGCTGATTC+TGG	0.358260	3.2:-69825441	None:intergenic
CTCATCAAGAATATTGTTAC+TGG	0.359156	3.2:+69820149	MS.gene25870:CDS
CTCAGCTTTCTTAATCCACT+TGG	0.360020	3.2:-69824384	None:intergenic
CTGTGGCATATGATAATCTT+TGG	0.361879	3.2:+69826180	MS.gene25870:CDS
ACTTACTCTTGTATGGTTCC+GGG	0.363410	3.2:-69825077	None:intergenic
TGTTTGAACTTACTCTTGTA+TGG	0.364028	3.2:-69825084	None:intergenic
TGTCCAATGTACTCTTCATC+TGG	0.364172	3.2:-69827005	None:intergenic
AATACTTCTGCCGTATCTTT+AGG	0.368151	3.2:+69824850	MS.gene25870:CDS
TTGGAGAAAATTCGAGTCAT+TGG	0.368346	3.2:-69824992	None:intergenic
CATTGTGCCTTTCCATCAAT+AGG	0.368981	3.2:-69825761	None:intergenic
ATAGTCACTGATATAAATCT+TGG	0.370327	3.2:+69824904	MS.gene25870:CDS
CCCACATTTCCAAAATTTGC+TGG	0.375304	3.2:-69822197	None:intergenic
ATTGAGGGAACAACACTATA+TGG	0.375357	3.2:+69825668	MS.gene25870:CDS
TTAGCATCCTTAAAGAACTT+TGG	0.376602	3.2:-69825024	None:intergenic
TCAATATTCCTTCACTAGTA+AGG	0.377393	3.2:-69825709	None:intergenic
AATATTGTTACTGGTATTGT+TGG	0.378392	3.2:+69820158	MS.gene25870:CDS
GACCTCAAAAGACCTGTTCT+TGG	0.381282	3.2:+69824519	MS.gene25870:CDS
TACCTATTGATAAGATCATT+TGG	0.383516	3.2:-69826224	None:intergenic
TCGAAAAGAATGATATTTCA+AGG	0.388151	3.2:-69826133	None:intergenic
GGGTGAGAATTCAACTATAA+AGG	0.389284	3.2:+69827046	MS.gene25870:CDS
ATATTGGTGGAATCAGAATT+AGG	0.390188	3.2:-69825800	None:intergenic
GAATCCATGATTGACAATTA+AGG	0.395271	3.2:-69822304	None:intergenic
GTACTCCTTTCTTCATATAG+TGG	0.399010	3.2:-69824769	None:intergenic
GGTCGAAAGCATTCTGATAC+AGG	0.402041	3.2:+69824582	MS.gene25870:CDS
CCACCAATATCTGGCTTTGG+AGG	0.402930	3.2:+69825813	MS.gene25870:CDS
AAGCCTCCAAAGCCAGATAT+TGG	0.404646	3.2:-69825816	None:intergenic
TCCACCTTAATTGTCAATCA+TGG	0.405732	3.2:+69822300	MS.gene25870:CDS
TTAAGGTGGACAGGGCCATC+AGG	0.407562	3.2:-69822287	None:intergenic
TAACCAATTGGATGCTATAT+GGG	0.408073	3.2:-69825265	None:intergenic
CTCACTAGACCACCTATTGA+TGG	0.408363	3.2:+69825749	MS.gene25870:CDS
ATTCCACCAATATCTGGCTT+TGG	0.411670	3.2:+69825810	MS.gene25870:CDS
AAATTGGATGCCCAAATCTA+TGG	0.413135	3.2:+69825414	MS.gene25870:CDS
CCATATTAGAAGACCGTCAT+TGG	0.419135	3.2:-69826556	None:intergenic
CATTCCAAAAGTGTTTGCTC+TGG	0.419934	3.2:-69826918	None:intergenic
TTCTGGTGGACCATAGATTT+GGG	0.421786	3.2:-69825424	None:intergenic
TCTAGGATATGGATATTGTT+TGG	0.425065	3.2:-69824547	None:intergenic
TATGATGTCTATAATGATCT+TGG	0.425109	3.2:+69824477	MS.gene25870:CDS
GTTTAAATGGATATGCTGTA+TGG	0.426545	3.2:-69827227	None:intergenic
GTCCTTGATGGTTTCCCTGA+TGG	0.427910	3.2:+69822272	MS.gene25870:CDS
TTGTAGGGGATAGATTCTTT+TGG	0.428079	3.2:+69825198	MS.gene25870:intron
CCAAAAGTGTTTGCTCTGGT+TGG	0.436594	3.2:-69826914	None:intergenic
TCCTTAGTAGGGTCTTCTGT+TGG	0.439100	3.2:-69826870	None:intergenic
TTTGTAGGAGTATCATGCTT+AGG	0.439282	3.2:-69822329	None:intergenic
TTTCCCATTCTTCCTTAGTA+GGG	0.440122	3.2:-69826881	None:intergenic
TTGAGGTCAGCGGTTATGTC+AGG	0.448323	3.2:-69824504	None:intergenic
ATGTCCATGCCTTAACCAAT+TGG	0.451067	3.2:-69825277	None:intergenic
AAGACCCTACTAAGGAAGAA+TGG	0.454559	3.2:+69826877	MS.gene25870:CDS
TCTCTCAGTTTGCTAACATA+TGG	0.455221	3.2:-69825644	None:intergenic
ATGGGATGCAATTAAACAAT+GGG	0.457493	3.2:+69826575	MS.gene25870:CDS
GGGCCTGGAGTCACTGGAAA+AGG	0.458638	3.2:+69827200	MS.gene25870:CDS
ATAGAAGCAATTTACAAAGA+AGG	0.458987	3.2:+69824946	MS.gene25870:CDS
ATGAACAAGAACATGAGCTT+TGG	0.461543	3.2:-69825840	None:intergenic
TAGACTCAAGGAATGAAGAT+AGG	0.461905	3.2:+69827117	MS.gene25870:CDS
AATGGCTTTAATGACTCATA+AGG	0.463312	3.2:-69827173	None:intergenic
GAATCTCTGTCCACCATGCT+TGG	0.465030	3.2:-69826659	None:intergenic
GGCACCAGCCAAAGTCTCTC+TGG	0.472557	3.2:-69825240	None:intergenic
TTCCAAATGATCTTATCAAT+AGG	0.473141	3.2:+69826222	MS.gene25870:CDS
CAATGACGGTCTTCTAATAT+GGG	0.476498	3.2:+69826557	MS.gene25870:CDS
CTAATAGAACTAAGAGGGAA+TGG	0.477908	3.2:+69824417	MS.gene25870:CDS
ATATAGCATCCAATTGGTTA+AGG	0.478204	3.2:+69825268	MS.gene25870:CDS
GATGAGCTAATAGAACTAAG+AGG	0.478619	3.2:+69824411	MS.gene25870:CDS
AGACCCTACTAAGGAAGAAT+GGG	0.480218	3.2:+69826878	MS.gene25870:CDS
TTTGCCAGAGAGACTTTGGC+TGG	0.481032	3.2:+69825236	MS.gene25870:CDS
ATAAGAGTAGTGATGGCAAA+AGG	0.486600	3.2:+69820053	None:intergenic
GATTGACAATTAAGGTGGAC+AGG	0.489484	3.2:-69822296	None:intergenic
TAGTCACTGATATAAATCTT+GGG	0.491052	3.2:+69824905	MS.gene25870:CDS
GGAATTTGCCAGAGAGACTT+TGG	0.496070	3.2:+69825232	MS.gene25870:CDS
TAGCAAACTGAGAGAGATTG+AGG	0.496616	3.2:+69825652	MS.gene25870:CDS
CTAGTAATTGCTGATTCTGG+TGG	0.502077	3.2:-69825438	None:intergenic
GATTCAAGAATGTCTGAGCT+TGG	0.509066	3.2:-69826624	None:intergenic
TTGAAGAATAGACATGGAGC+TGG	0.509740	3.2:+69827143	MS.gene25870:CDS
CAACCCATATAGCATCCAAT+TGG	0.511275	3.2:+69825262	MS.gene25870:CDS
ATCATGAACTTATAAGCCAT+TGG	0.514400	3.2:+69825873	MS.gene25870:CDS
TACTCCTTTCTTCATATAGT+GGG	0.517291	3.2:-69824768	None:intergenic
AGAAGACCGTCATTGGCAAA+AGG	0.518247	3.2:-69826549	None:intergenic
AGATGCTGTCCAAGCTATAG+TGG	0.518404	3.2:-69826763	None:intergenic
AATGTTGCTTGAATTTGTGA+TGG	0.521479	3.2:-69826945	None:intergenic
AGGAGATTGAATGAAATTGA+AGG	0.525552	3.2:+69827089	MS.gene25870:CDS
CCTCAAAAGACCTGTTCTTG+GGG	0.526691	3.2:+69824521	MS.gene25870:CDS
ACATCATTACCACTATAGCT+TGG	0.532589	3.2:+69826754	MS.gene25870:CDS
GAACTAAGAGGGAATGGAGA+AGG	0.533603	3.2:+69824423	MS.gene25870:CDS
AACTTATAGCACAGTTGAAG+AGG	0.540223	3.2:+69825485	MS.gene25870:CDS
TTTAAATGGATATGCTGTAT+GGG	0.542203	3.2:-69827226	None:intergenic
GCCAACAGAAGACCCTACTA+AGG	0.544940	3.2:+69826869	MS.gene25870:CDS
CAGACCCACTATATGAAGAA+AGG	0.547760	3.2:+69824764	MS.gene25870:intron
CAATTGGATGCTATATGGGT+TGG	0.548678	3.2:-69825261	None:intergenic
TGTAGTCAAGCCTAAAGATA+CGG	0.551843	3.2:-69824860	None:intergenic
CGCAACTTTATTGATCTCCA+TGG	0.555615	3.2:-69826081	None:intergenic
AATCCACTTGGTGTTTGTGA+TGG	0.558499	3.2:-69824372	None:intergenic
GGCCATCAGGGAAACCATCA+AGG	0.563223	3.2:-69822274	None:intergenic
ATCATAATCATAGATTCTGT+CGG	0.564842	3.2:-69824460	None:intergenic
CGACCGGTGCGACACCGTCT+AGG	0.566262	3.2:-69824564	None:intergenic
TATGGGATGCAATTAAACAA+TGG	0.570977	3.2:+69826574	MS.gene25870:CDS
CCTCCAAAGCCAGATATTGG+TGG	0.572821	3.2:-69825813	None:intergenic
GAAGTTCTCATTAATGCAAG+TGG	0.573211	3.2:+69826107	MS.gene25870:CDS
TTGAAGGTATTATAGACTCA+AGG	0.576809	3.2:+69827105	MS.gene25870:CDS
TAAGGTGGACAGGGCCATCA+GGG	0.582528	3.2:-69822286	None:intergenic
ATGAGCTAATAGAACTAAGA+GGG	0.582546	3.2:+69824412	MS.gene25870:CDS
CTTACTCTTGTATGGTTCCG+GGG	0.587013	3.2:-69825076	None:intergenic
GTTGAATTCTCACCCCAAGA+TGG	0.587735	3.2:-69827038	None:intergenic
TAGACCACCTATTGATGGAA+AGG	0.590035	3.2:+69825754	MS.gene25870:CDS
TATCCTAGACGGTGTCGCAC+CGG	0.590911	3.2:+69824561	MS.gene25870:CDS
AGCAAACTGAGAGAGATTGA+GGG	0.591240	3.2:+69825653	MS.gene25870:CDS
TGTATCAGAATGCTTTCGAC+CGG	0.595298	3.2:-69824580	None:intergenic
AACAATATCCATATCCTAGA+CGG	0.598592	3.2:+69824550	MS.gene25870:CDS
TGTGCCTTTCCATCAATAGG+TGG	0.601759	3.2:-69825758	None:intergenic
TTGCCATCACAAACACCAAG+TGG	0.605543	3.2:+69824369	MS.gene25870:CDS
CATCCTTTGAAAAGTTACAA+AGG	0.609574	3.2:+69827069	MS.gene25870:CDS
GCATGAAGAAGCAAACTCGA+AGG	0.610739	3.2:+69822097	MS.gene25870:intron
TCAGCAAATGCCTCGTCACG+AGG	0.615936	3.2:-69824808	None:intergenic
CTTCTATGTTCCTCGTGACG+AGG	0.615973	3.2:+69824798	MS.gene25870:CDS
TCCATGGTATATCTCAGATG+TGG	0.618261	3.2:-69826065	None:intergenic
CTAGTGAGCTCAATAGCTAG+TGG	0.619729	3.2:-69825734	None:intergenic
TCACCAGATGAAGAGTACAT+TGG	0.620155	3.2:+69827002	MS.gene25870:CDS
CTCTCTGGCAAATTCCACAT+CGG	0.621861	3.2:-69825225	None:intergenic
CATGAAGAAGCAAACTCGAA+GGG	0.623114	3.2:+69822098	MS.gene25870:intron
TCCATGATTGACAATTAAGG+TGG	0.624709	3.2:-69822301	None:intergenic
TATCGTGCGCTTTGATACCC+CGG	0.626726	3.2:+69825059	MS.gene25870:CDS
GCTAAGAACTCATTGTGTTG+TGG	0.627066	3.2:+69825977	MS.gene25870:CDS
CTGATCAAGAGCTCCAAGCA+TGG	0.628834	3.2:+69826646	MS.gene25870:CDS
TGGACAGAGATTCGAACAGT+GGG	0.633544	3.2:+69826669	MS.gene25870:CDS
AGGAATTTGAAGAATAGACA+TGG	0.634907	3.2:+69827137	MS.gene25870:CDS
CAGTAACAATATTCTTGATG+AGG	0.640270	3.2:-69820148	None:intergenic
AAAAGCTTTAGTGAGTGTGA+AGG	0.645031	3.2:+69820112	MS.gene25870:CDS
ATTGACAATTAAGGTGGACA+GGG	0.652090	3.2:-69822295	None:intergenic
CGGACAAATCTGAAGAACCA+TGG	0.653620	3.2:+69826697	MS.gene25870:CDS
TCCACATCTGAGATATACCA+TGG	0.656207	3.2:+69826064	MS.gene25870:CDS
CTATTGATGGAAAGGCACAA+TGG	0.657974	3.2:+69825762	MS.gene25870:CDS
CTCAAAAGACCTGTTCTTGG+GGG	0.662000	3.2:+69824522	MS.gene25870:CDS
TACAATGGAGTTAAGCTCTG+TGG	0.664172	3.2:+69826163	MS.gene25870:CDS
GAAGATCCTAATGCACAACA+TGG	0.666470	3.2:+69826501	MS.gene25870:CDS
ATCAAGAGCTCCAAGCATGG+TGG	0.666595	3.2:+69826649	MS.gene25870:CDS
GATTCGAACAGTGGGACATG+CGG	0.669148	3.2:+69826677	MS.gene25870:CDS
GTGGACAGAGATTCGAACAG+TGG	0.669638	3.2:+69826668	MS.gene25870:CDS
GTGAGTGTGAAGGAAAGTGA+TGG	0.706550	3.2:+69820122	MS.gene25870:CDS
AGTGTGAAGGAAAGTGATGG+TGG	0.718611	3.2:+69820125	MS.gene25870:CDS
ACAAATCTGAAGAACCATGG+TGG	0.731842	3.2:+69826700	MS.gene25870:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	69820065	69827247	69820065	ID=MS.gene25870
chr3.2	mRNA	69820065	69827247	69820065	ID=MS.gene25870.t1;Parent=MS.gene25870
chr3.2	exon	69820065	69820227	69820065	ID=MS.gene25870.t1.exon1;Parent=MS.gene25870.t1
chr3.2	CDS	69820065	69820227	69820065	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69822105	69822373	69822105	ID=MS.gene25870.t1.exon2;Parent=MS.gene25870.t1
chr3.2	CDS	69822105	69822373	69822105	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69824363	69824603	69824363	ID=MS.gene25870.t1.exon3;Parent=MS.gene25870.t1
chr3.2	CDS	69824363	69824603	69824363	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69824767	69825093	69824767	ID=MS.gene25870.t1.exon4;Parent=MS.gene25870.t1
chr3.2	CDS	69824767	69825093	69824767	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69825204	69825289	69825204	ID=MS.gene25870.t1.exon5;Parent=MS.gene25870.t1
chr3.2	CDS	69825204	69825289	69825204	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69825396	69825506	69825396	ID=MS.gene25870.t1.exon6;Parent=MS.gene25870.t1
chr3.2	CDS	69825396	69825506	69825396	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69825593	69825894	69825593	ID=MS.gene25870.t1.exon7;Parent=MS.gene25870.t1
chr3.2	CDS	69825593	69825894	69825593	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69825977	69826243	69825977	ID=MS.gene25870.t1.exon8;Parent=MS.gene25870.t1
chr3.2	CDS	69825977	69826243	69825977	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1
chr3.2	exon	69826488	69827247	69826488	ID=MS.gene25870.t1.exon9;Parent=MS.gene25870.t1
chr3.2	CDS	69826488	69827247	69826488	ID=cds.MS.gene25870.t1;Parent=MS.gene25870.t1

Gene Sequence

>MS.gene25870

ATGGCAAAAGGAAGTAGCAGCAGTGACAACAAATCTCATCATAATGTAAAAGCTTTAGTGAGTGTGAAGGAAAGTGATGGTGGCCTCATCAAGAATATTGTTACTGGTATTGTTGGTAACAAACACTTAATTTTGGAGTTAGTCAGTGCTGAGCTTGATCCAAGTAAGTTTATGAGATTAATTTTAATTAATTGTGTCTGTATAAAAAATTATACCATTGACAAATCATAATTATTCATGCTTGTTACTTTAAAAATAGTCATTTTTAAGATGATCTCATGATTGATTGACAATGTGAAAAAACATTTAAATTGAAAGTGTATGCAGTATACACCCTCCGGTCACAAATATAAGCAAAAATCCATGTTTTAAATTCATTCAATAAATGATGTATCTAGTCTTTATTGTAGACAACATACATCATTTATTGAATGAATCTATAATATTGATTTTTGCTTATATTTGTGACCGGATGGTGTATAATTTAAGAGTTCAGATGGGCATATAATGCGAGCCCTCCATCTTTCCATGTCACAACTTAACTATCAGTTGCAAAAGTGCTCAAAAAACATCATTAACAAAAGTGGAGTGTCATAAACCAAACAATTGAACTCAAATAGTTAATGAGTTTTACCAATGACGAATCGTTTGTGAGAATCCAAATTCGATGGTAGAATTTTGATTAACCTATTCTTACCTCACAACCAAACTTTAAATTACTATGACCCTTTTCCGTTAGGAAACGAAGAGTTAACGCAAAAAAAAAATGTGGAGTGTCATAACAAAGTAGTATATTTTTATGAGATGATTTTTTTAACACCCTAAAAAATTTTACACCAATTTAACACGTTTTATTCTCTTTGTCTCTCTTCACCACCTTCCGATCATATTTCTCTCCCCCTCTCTCTTTGTCTATCTCTCTGATCTCAATATCTCTTAAGGTGTATAATAACATTGGGTGTGCAAGGAATGTTTTTCTTATTTTTATGTGTAAAATTAATTTACAAATGATGATGCATATCTTTTAAATCCATAAATTAAACGCCAAGTATATTTATCTATTGATTTATCTATATGATGATAAAAAATGGCAGCCATAAAGTATCCATCTATTATAGAATTTTTTTATAGAATATATTATTACAAATATAAAGTCGTCAAAATGGATTATTTTTCTTTTTATAAAAGACAAAACAACAACGGTCAACGTCAAAAAAAAAAAAAACAACAACGGTCAACAAGACTTCCGTCAACAAAAAAAAAAAAAAAGGCTCACAAAAGTGGTATAAAGTGTTATTCAAAATAAATGTGATTGTTTTACACATAAATAAATAAATAAAATATTGAAATAAAGAAAATGATAATTTAACCAAATGATCTTAGTTAGACCATTTCCAATGGAGGATCCTGACACGTACTCCATCGGGAAGATAAAATTTAGGGGTCTTATAGGACCCATGGTCTTACTTAAGAGTTAAGACCTTATTCTTAAGTGAGATCAACACTTTTTTAATATTAAAAAATTGACATATAATGATTTAAAATTATTGATAGTTGATTAATATGTGTCCTATTTAAGAAATTTGTGTGAGACGCTGAGTTGGAGGGATTGAGTGCTTATTAGATACTACTATTAAAAAATTATAAATGAGTGATGTGTACAAAAAATAGATGCCTTCGTTGTGAATGTTTTTAAGTATTGATATATCCACCATTGTCATGAATCATTTGTCAACATTTAAGGCTTAGAGCTAAATTTCTTTAGTTTGGTAAAGTAACTACCAAAATGTGAACCCATTTGGTTGGTCTCTTGAAACCTTGGAGAATACTTCTCTCAATCCGTTTCTTCCCGCTACTAATTTTGATCGACTAGTTTGATTTAAAAAAAAATCATTTTAAGAGTTTTTTTTAAATGCAACATATATTATGAGACATTGGTAATATATAGTATATACACACCACAATTAATTTATATGCATTCGTGTATCAAAATTGACTTTGATTCTCTTTTTATTTCCCCGCAACCTTATTTGCATGAAGAAGCAAACTCGAAGGGAGAGACAATAAAAAGAACCGCACACGAAACAGAGAAAAAAGAGAAAGAGGTGCAGTATGAAGCAACATTTGAGTTACCAGCAAATTTTGGAAATGTGGGTGCTGTTTTGGTTGAGAATGAACATGACAAAGAGATATTTCTAAAGAATATAGTCCTTGATGGTTTCCCTGATGGCCCTGTCCACCTTAATTGTCAATCATGGATTCAGCCTAAGCATGATACTCCTACAAAGAGAGTTTTCTTCATTAACAAGGTAACGATGTTAATACATTAGGTCTTATTCACTAACACACTCATGCATATGGGTCGCAATAAATTTTGTTGGGACCCACCTAATTGGATAGGATTTATATGAATTTCAACTAAAAGAAAAATGTGAATAGAAATTGGATTTGGATGTTGCTGTCACATGCCATCCAAACATCGGTGATTGTTGATCTCGCACAAATGGTTTAAAACTGTAAATTTAATTTTGCCTAAGCATGATACTCCTACAAAGAGAGTTTTCTTCATTAACAAGGTAACGATGTTAATACATTAGGTCTTATTCACTAACACACTCATGCATATGGGTCGCAATAAATTTTGTTGGGACCCACCTAATTGGATAGGATTTATATGAATTTCAACTAAAAGAAAAATGTGAATAGAAATTGGATTTGGTGGTTGCTGTCACATGCCATCCAAACATCGGTGATTGTTGATCTCGCACAAATGGTTTAAAACTGTAAATTTAATTTTTATTGTATAATTTAAAAAATAGAGTTTGAATTATGACAGTCTGATCTTAATCCAACAACTACTGGTGTGTGACTGCGTCAAATTGTTAACTCCGGCAGATCTAGATTCGTGTTGGAATGTGTGAATGTAGCATTAATATTATGCTTGTTATTTTATTAGTTGAGAAGTTGAACCCATGAATTTCTTATTAGTAAACTCTTAAGCAAGCAAGTCAGGGGTTCGAATACTGACTCTTGTGTACGGAGAAAATTCGGTTGAGATGAGAGGACCCATCTTGTATGTTCAATAGGTTTGCGACAAAGATTTTTTACCACTAAACAAGGTGGAAGTTTCATACCTATATCGTGGTAATCCAAAAAAACTATCAAGCCATTGTTGATAGACTAGTTGATTTTTCAATATAAGCAGGAAATTATTTAACCCAACATTTGAAACACACAAGAAAAACTTTCAATTTAATTTCTCCACATCTCCTCCCATTCCCTAGTCCCAACTATATCCTTGTTGAGTCGTGACTCACGAGGAGAGTCTTACGGTCCTGGGATCATCACATCGACCTTTGAGTTTGCTCAAAACTTTAAGGCATTTGACATATGGCTCATCTCACTTGTGAGATTGTTATCACACTTGATATATGGACAATACGTAGTACCTGTTTGGCATAGCGTTTTCCCATCTCAAACCGGAGTTTAGAGGCTGAAACGCAGTTTCAACATTTTCTTAGGTGTTTGGATAACTGCAAACGGGATTCTACCAAACGTGGATCTAGCCCCCAACCGGCGTTTCTTAAAATCTGGAAAATGAATCTTTTGCGGCAACTAAAAGCTGGTTTGGAATTTAATCTATGATGACAAATTGGAAATTCCCTAATTACCCATGACTCAGTTTTCTATTACCAGGTTCCTCCGTTTCAATAGCACAAGCCACAACGATCCTTAAACCATCTCATAAATTTGGAAATTTGTAATATTGTTTCATGGGTAATATATAACTCTTATAAATTAATATTATATTAAAATGAATTGACTTAATAATGCAACATAAATTTGAACCAGTATTCATTTTTTAATTTATTTACTAAAATACAATTTTCATTTTGTTTTAAAAGTTTTATTAATCTACAAATAATTGACTTAATAATGCAAAATAAATTTGAACCATTATATAAAATAAAAATTAAGTTTATTAAAGAAAAATGAAGTCTAATTTTAAATAGATATTAATACCCCTTTTGGTAATTTTGCGTTCAAAATCAATTTTGATTCAAACTATCCAAACAACATCTACTCATAAGAATCACTTTTAGTAAAGTGTATCCAAACATAATCAATTCACATTCAACTCACTTTTAACCAAAATCAATTCTCTCAAACTCAATTCTATCAAAATCAATTCTCCCCACCGCCATACCAAACACACACGTATATAGTTGCATATCTCATTTTTTGTTTACATGACCTGATTTAATTTGTATGTTATTTTTCATGCATGGAATTAATAGATGTATTTGCCATCACAAACACCAAGTGGATTAAGAAAGCTGAGAGAAGATGAGCTAATAGAACTAAGAGGGAATGGAGAAGGAGAGCGTAAGAAATCCGACAGAATCTATGATTATGATGTCTATAATGATCTTGGAGATCCTGACATAACCGCTGACCTCAAAAGACCTGTTCTTGGGGGCACCAAACAATATCCATATCCTAGACGGTGTCGCACCGGTCGAAAGCATTCTGATACAGGTTACCTATTCTCAATGCTTACATATCTTTTTCTCAAAAATGCTCATAATATTACTTATTCTTCAAGCAATAACTTTGATTGCATATATTTCTCTTAATTGGTATAATTGTGGAGATATTTCAAGATATATATATGTTTATAGCACAATATTTTTTTATACAGACCCACTATATGAAGAAAGGAGTACTTTAGACTTCTATGTTCCTCGTGACGAGGCATTTGCTGAAACAAAGCAGACACAATTCAATACTTCTGCCGTATCTTTAGGCTTGACTACAATTATTCAATCCTTAGACGCAATAGTCACTGATATAAATCTTGGGTTTGAAAACTTTGAAGACATAGAAGCAATTTACAAAGAAGGATTTCAGTTACCAAATCTTGAATCCAATGACTCGAATTTTCTCCAAAAACTCATTCCAAAGTTCTTTAAGGATGCTAACGATAGCCAAAATATCGTGCGCTTTGATACCCCGGAACCATACAAGAGTAAGTTCAAACATATAAATGAGATTTATTTGATATAAACTTAACCTGTCATGAAATGGAAATTCTTTTCTGGTTTCTCATCAATGTGAATATGTGCCTCGGAATTGTAGGGGATAGATTCTTTTGGTTTTCCGATGTGGAATTTGCCAGAGAGACTTTGGCTGGTGCCAACCCATATAGCATCCAATTGGTTAAGGCATGGACATTACTACAAATTAATATTCATTTCACTCCTTTCACAATAACTTGTATATATGTTCAATAATTTTCCTAGTTTTTTTTTTTTTTTGTTCATTTGATAGGAGTGGCCTTTGAAAAGTAAATTGGATGCCCAAATCTATGGTCCACCAGAATCAGCAATTACTAGAGAGATCATTGAGTCACAAATCACAACTTATAGCACAGTTGAAGAGGTAGAAAGAGCAACATTTTTTTTTTTTTACTAAATTAATAATAATGTATAATGCTGATTTTTTTTTTGTTAAATTCAATACTACAGGCTATTAAAGAGAAGAAGTTGTTCATGCTAGACTATCATGATTTTTACTTACCATATGTTAGCAAACTGAGAGAGATTGAGGGAACAACACTATATGGATCAAGAACACTATTTTTCCTTACTAGTGAAGGAATATTGAAGCCACTAGCTATTGAGCTCACTAGACCACCTATTGATGGAAAGGCACAATGGAAGCAAGTGTTCACACCTAATTCTGATTCCACCAATATCTGGCTTTGGAGGCTTGCCAAAGCTCATGTTCTTGTTCATGATTCTGGTCATCATGAACTTATAAGCCATTGGTTAGTACCAATTAGGTTGTATAAGATGTTATGTCAAGCCTATAACTAGAACTAACTTATTATATGTTAATTGTGTTTGCAGGCTAAGAACTCATTGTGTTGTGGAACCTATTATAATTGCAACACACAGACAACTCAGTTCCATGCATCCAATTTTTAGATTATTGCATCCACATCTGAGATATACCATGGAGATCAATAAAGTTGCGCGTGAAGTTCTCATTAATGCAAGTGGACTCCTTGAAATATCATTCTTTTCGAATAAGTATACAATGGAGTTAAGCTCTGTGGCATATGATAATCTTTGGCAATTTGATTTGCAGGCACTTCCAAATGATCTTATCAATAGGTAATGTTTATAATGATGCTGAATATTATGCTATGAAGCACTAAATCAGACACCAGATAGGACACTAATACTGACATGTTGACAATCACAATAATTTGAGAAATTGAAAGTAATTGAATGTAAACATGTCTTGGTTTCGGACACAGACATGTGTCAGTGCTACATAGATATTATGTCTGTTTGCGTTTCCTTCTTTATGAAAACCACATTGAATATTAACTTACGTAATTGTTATTTTTCTAAGAGGAATGGCTGTGGAAGATCCTAATGCACAACATGGAGTGAAGCTAATAATTGAAGACTATCCTTTTGCCAATGACGGTCTTCTAATATGGGATGCAATTAAACAATGGGTTACAGAATATGTCAATCATTACTATCCAAGCTCAGACATTCTTGAATCTGATCAAGAGCTCCAAGCATGGTGGACAGAGATTCGAACAGTGGGACATGCGGACAAATCTGAAGAACCATGGTGGCCAAATCTCAAAACACAAAATGATCTTATTAACATCATTACCACTATAGCTTGGACAGCATCTGCTCATCATTCAGCTGTTAACTTTACACAATATGCTTATATAGGCTTTTTCCCTAATAGACCAACAATTGCTAGAAAGAAAATGCCAACAGAAGACCCTACTAAGGAAGAATGGGAAAAATTTATGAACCAACCAGAGCAAACACTTTTGGAATGTTTTCCATCACAAATTCAAGCAACATTATTTATGATTGTTATGAGCATATTATCAGAACATTCACCAGATGAAGAGTACATTGGACAAAAAATAGAGCCATCTTGGGGTGAGAATTCAACTATAAAGGCATCCTTTGAAAAGTTACAAAGGAGATTGAATGAAATTGAAGGTATTATAGACTCAAGGAATGAAGATAGGAATTTGAAGAATAGACATGGAGCTGGAATAGTGCCTTATGAGTCATTAAAGCCATTTTCAGGGCCTGGAGTCACTGGAAAAGGAGTCCCATACAGCATATCCATTTAA

Protein sequence

>MS.gene25870.t1

MAKGSSSSDNKSHHNVKALVSVKESDGGLIKNIVTGIVGNKHLILELVSAELDPKANSKGETIKRTAHETEKKEKEVQYEATFELPANFGNVGAVLVENEHDKEIFLKNIVLDGFPDGPVHLNCQSWIQPKHDTPTKRVFFINKMYLPSQTPSGLRKLREDELIELRGNGEGERKKSDRIYDYDVYNDLGDPDITADLKRPVLGGTKQYPYPRRCRTGRKHSDTDPLYEERSTLDFYVPRDEAFAETKQTQFNTSAVSLGLTTIIQSLDAIVTDINLGFENFEDIEAIYKEGFQLPNLESNDSNFLQKLIPKFFKDANDSQNIVRFDTPEPYKRDRFFWFSDVEFARETLAGANPYSIQLVKEWPLKSKLDAQIYGPPESAITREIIESQITTYSTVEEAIKEKKLFMLDYHDFYLPYVSKLREIEGTTLYGSRTLFFLTSEGILKPLAIELTRPPIDGKAQWKQVFTPNSDSTNIWLWRLAKAHVLVHDSGHHELISHWLRTHCVVEPIIIATHRQLSSMHPIFRLLHPHLRYTMEINKVAREVLINASGLLEISFFSNKYTMELSSVAYDNLWQFDLQALPNDLINRGMAVEDPNAQHGVKLIIEDYPFANDGLLIWDAIKQWVTEYVNHYYPSSDILESDQELQAWWTEIRTVGHADKSEEPWWPNLKTQNDLINIITTIAWTASAHHSAVNFTQYAYIGFFPNRPTIARKKMPTEDPTKEEWEKFMNQPEQTLLECFPSQIQATLFMIVMSILSEHSPDEEYIGQKIEPSWGENSTIKASFEKLQRRLNEIEGIIDSRNEDRNLKNRHGAGIVPYESLKPFSGPGVTGKGVPYSISI