AlfalfaGEDB | MS.gene26647

M. sativa cultivar XinJiangDaYe / MS.gene26647

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004866 GO:0004869 GO:0030234 GO:0030414 GO:0061134 GO:0061135 GO:0098772
PFAM: PF16845.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26647.t1	PNY11489.1	62.5	88	31	1	3	90	42	127	1.30E-20	109

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26647.t1	Q8H0X6	50.6	79	37	1	13	91	51	127	8.9e-18	90.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26647.t1	A0A2K3P878	62.5	88	31	1	3	90	42	127	9.5e-21	109.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene26647	0.809782	1.60E-50	-1.69E-46
MS.gene050026	MS.gene26647	0.823965	1.04E-53	-1.69E-46
MS.gene052519	MS.gene26647	0.832025	1.20E-55	-1.69E-46
MS.gene053006	MS.gene26647	0.800934	1.14E-48	-1.69E-46
MS.gene05415	MS.gene26647	0.802118	6.53E-49	-1.69E-46
MS.gene054651	MS.gene26647	0.83613	1.12E-56	-1.69E-46
MS.gene055228	MS.gene26647	0.819435	1.16E-52	-1.69E-46
MS.gene05531	MS.gene26647	0.814157	1.78E-51	-1.69E-46
MS.gene055818	MS.gene26647	0.802591	5.22E-49	-1.69E-46
MS.gene056366	MS.gene26647	0.814957	1.18E-51	-1.69E-46
MS.gene05686	MS.gene26647	0.805547	1.27E-49	-1.69E-46
MS.gene05690	MS.gene26647	0.817927	2.56E-52	-1.69E-46
MS.gene057208	MS.gene26647	0.815591	8.55E-52	-1.69E-46
MS.gene058009	MS.gene26647	0.820544	6.49E-53	-1.69E-46
MS.gene058625	MS.gene26647	0.804056	2.59E-49	-1.69E-46
MS.gene058781	MS.gene26647	0.802064	6.70E-49	-1.69E-46
MS.gene058783	MS.gene26647	0.816873	4.42E-52	-1.69E-46
MS.gene058979	MS.gene26647	0.809224	2.10E-50	-1.69E-46
MS.gene059272	MS.gene26647	0.819745	9.88E-53	-1.69E-46
MS.gene059584	MS.gene26647	0.815868	7.42E-52	-1.69E-46
MS.gene060011	MS.gene26647	0.813687	2.26E-51	-1.69E-46
MS.gene060258	MS.gene26647	0.802585	5.23E-49	-1.69E-46
MS.gene061091	MS.gene26647	0.815373	9.56E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26647.t1	MTR_8g041410	51.111	90	37	2	13	97	56	143	1.19e-25	95.9
MS.gene26647.t1	MTR_5g088770	55.263	76	32	1	13	88	18	91	6.78e-24	87.8
MS.gene26647.t1	MTR_3g043750	53.846	78	34	1	14	91	19	94	4.20e-22	83.2
MS.gene26647.t1	MTR_3g451210	41.250	80	45	1	12	91	44	121	9.68e-13	60.1
MS.gene26647.t1	MTR_3g452280	41.250	80	45	1	12	91	44	121	9.68e-13	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26647.t1	AT3G12490	48.780	82	40	1	10	91	15	94	1.15e-24	91.3
MS.gene26647.t1	AT3G12490	48.780	82	40	1	10	91	48	127	4.36e-24	91.7
MS.gene26647.t1	AT5G05110	53.488	86	37	2	13	97	60	143	4.38e-24	91.7
MS.gene26647.t1	AT2G40880	47.436	78	39	1	10	87	46	121	5.12e-22	83.6
MS.gene26647.t1	AT5G12140	37.805	82	49	1	10	91	21	100	5.38e-15	65.1

Find 23 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCATGGACGCACCCATATTT+TGG	0.177930	7.2:-20017122	None:intergenic
TTCATATATCTTCCTCTTTC+CGG	0.178135	7.2:-20017098	None:intergenic
ACAGCGAACCTTTGGATTTC+AGG	0.190327	7.2:-20016805	None:intergenic
AGCTGCTGCAACCTGTTCTT+TGG	0.234170	7.2:-20017041	None:intergenic
AGATATATGAAGCCAAAATA+TGG	0.271985	7.2:+20017110	MS.gene26647:CDS
TTCCGGCATCAACTACTTCC+AGG	0.310229	7.2:-20017081	None:intergenic
ATCATCGATTGGAACTTCTT+TGG	0.323935	7.2:-20016782	None:intergenic
CTGCGGAAACAGCGAACCTT+TGG	0.395309	7.2:-20016813	None:intergenic
CCAAAATATGGGTGCGTCCA+TGG	0.432048	7.2:+20017122	MS.gene26647:CDS
TACTATTAGACAATATGTAA+TGG	0.436800	7.2:+20017209	MS.gene26647:CDS
TGACGTTCTTGAATTTGTAA+AGG	0.484943	7.2:+20017009	MS.gene26647:CDS
GATATATGAAGCCAAAATAT+GGG	0.489904	7.2:+20017111	MS.gene26647:CDS
TGGATTTCAGGATCATCGAT+TGG	0.495612	7.2:-20016793	None:intergenic
GGTATTGAAAGCCAAAGAAC+AGG	0.495824	7.2:+20017030	MS.gene26647:CDS
TGAACAACACAACATCGAAA+AGG	0.541701	7.2:+20016837	MS.gene26647:CDS
AAGTAGTTGATGCCGGAAAG+AGG	0.572431	7.2:+20017086	MS.gene26647:CDS
TCCGGCATCAACTACTTCCA+GGG	0.573743	7.2:-20017080	None:intergenic
ACCCTGGAAGTAGTTGATGC+CGG	0.581195	7.2:+20017079	MS.gene26647:CDS
TCGATGATCCTGAAATCCAA+AGG	0.630004	7.2:+20016797	MS.gene26647:CDS
GTCATGTTCACATTCATCCA+TGG	0.651695	7.2:-20017139	None:intergenic
GATGTTGTGTTGTTCAACTG+CGG	0.676348	7.2:-20016830	None:intergenic
TAGAATATATCATATCACCC+TGG	0.718228	7.2:+20017063	MS.gene26647:CDS
GATGAATGTGAACATGACGC+AGG	0.732308	7.2:+20017144	MS.gene26647:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTAAATCTTAGATGTT+AGG	-	chr7.2:20016942-20016961	None:intergenic	15.0%
!!!	ATCTAAGATTTAATTATTCA+AGG	+	chr7.2:20016945-20016964	MS.gene26647:intron	15.0%
!!!	TCTAAGATTTAATTATTCAA+GGG	+	chr7.2:20016946-20016965	MS.gene26647:intron	15.0%
!	AGATATATGAAGCCAAAATA+TGG	+	chr7.2:20017110-20017129	MS.gene26647:CDS	25.0%
!	GATATATGAAGCCAAAATAT+GGG	+	chr7.2:20017111-20017130	MS.gene26647:CDS	25.0%
	TAGAATATATCATATCACCC+TGG	+	chr7.2:20017063-20017082	MS.gene26647:CDS	30.0%
	TGACGTTCTTGAATTTGTAA+AGG	+	chr7.2:20017009-20017028	MS.gene26647:CDS	30.0%
	TTCATATATCTTCCTCTTTC+CGG	-	chr7.2:20017101-20017120	None:intergenic	30.0%
!!	ATCGAAAAGGTATTCATCAA+GGG	+	chr7.2:20016850-20016869	MS.gene26647:intron	30.0%
	TGAACAACACAACATCGAAA+AGG	+	chr7.2:20016837-20016856	MS.gene26647:CDS	35.0%
!	ATCATCGATTGGAACTTCTT+TGG	-	chr7.2:20016785-20016804	None:intergenic	35.0%
!!	CATCGAAAAGGTATTCATCA+AGG	+	chr7.2:20016849-20016868	MS.gene26647:intron	35.0%
	GTCATGTTCACATTCATCCA+TGG	-	chr7.2:20017142-20017161	None:intergenic	40.0%
	TCGATGATCCTGAAATCCAA+AGG	+	chr7.2:20016797-20016816	MS.gene26647:CDS	40.0%
	TGGATTTCAGGATCATCGAT+TGG	-	chr7.2:20016796-20016815	None:intergenic	40.0%
!	GATGTTGTGTTGTTCAACTG+CGG	-	chr7.2:20016833-20016852	None:intergenic	40.0%
!	GGTATTGAAAGCCAAAGAAC+AGG	+	chr7.2:20017030-20017049	MS.gene26647:CDS	40.0%
!	TTTTCTTTCTCTGCCAAGTC+TGG	+	chr7.2:20016903-20016922	MS.gene26647:intron	40.0%
	ACAGCGAACCTTTGGATTTC+AGG	-	chr7.2:20016808-20016827	None:intergenic	45.0%
	CCAAGTCTGGATTATGCAAC+AGG	+	chr7.2:20016916-20016935	MS.gene26647:intron	45.0%
	CCTGTTGCATAATCCAGACT+TGG	-	chr7.2:20016919-20016938	None:intergenic	45.0%
	GATGAATGTGAACATGACGC+AGG	+	chr7.2:20017144-20017163	MS.gene26647:CDS	45.0%
!	AAGTAGTTGATGCCGGAAAG+AGG	+	chr7.2:20017086-20017105	MS.gene26647:CDS	45.0%
	AGCTGCTGCAACCTGTTCTT+TGG	-	chr7.2:20017044-20017063	None:intergenic	50.0%
	CCAAAATATGGGTGCGTCCA+TGG	+	chr7.2:20017122-20017141	MS.gene26647:CDS	50.0%
	TCCGGCATCAACTACTTCCA+GGG	-	chr7.2:20017083-20017102	None:intergenic	50.0%
	TTCCGGCATCAACTACTTCC+AGG	-	chr7.2:20017084-20017103	None:intergenic	50.0%
!	ACCCTGGAAGTAGTTGATGC+CGG	+	chr7.2:20017079-20017098	MS.gene26647:CDS	50.0%
!	CCATGGACGCACCCATATTT+TGG	-	chr7.2:20017125-20017144	None:intergenic	50.0%
	CTGCGGAAACAGCGAACCTT+TGG	-	chr7.2:20016816-20016835	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	20016772	20017228	20016772	ID=MS.gene26647
chr7.2	mRNA	20016772	20017228	20016772	ID=MS.gene26647.t1;Parent=MS.gene26647
chr7.2	exon	20016772	20016858	20016772	ID=MS.gene26647.t1.exon1;Parent=MS.gene26647.t1
chr7.2	CDS	20016772	20016858	20016772	ID=cds.MS.gene26647.t1;Parent=MS.gene26647.t1
chr7.2	exon	20017007	20017228	20017007	ID=MS.gene26647.t1.exon2;Parent=MS.gene26647.t1
chr7.2	CDS	20017007	20017228	20017007	ID=cds.MS.gene26647.t1;Parent=MS.gene26647.t1

Gene Sequence

>MS.gene26647

ATGAGAGCAGCCAAAGAAGTTCCAATCGATGATCCTGAAATCCAAAGGTTCGCTGTTTCCGCAGTTGAACAACACAACATCGAAAAGGTATTCATCAAGGGTTTATATTATTCTTAGCTCATTGATTTAATTTTTCTTTCTCTGCCAAGTCTGGATTATGCAACAGGCCTAACATCTAAGATTTAATTATTCAAGGGTTTATACATTTATTTACATATTTATTTCTTTTATGCAGAATGACGTTCTTGAATTTGTAAAGGTATTGAAAGCCAAAGAACAGGTTGCAGCAGCTAGAATATATCATATCACCCTGGAAGTAGTTGATGCCGGAAAGAGGAAGATATATGAAGCCAAAATATGGGTGCGTCCATGGATGAATGTGAACATGACGCAGGTGCAAGAATTCAAACTTGCTCATGACTTCTGTCAAGATGATTTACTATTAGACAATATGTAA

Protein sequence

>MS.gene26647.t1

MRAAKEVPIDDPEIQRFAVSAVEQHNIEKNDVLEFVKVLKAKEQVAAARIYHITLEVVDAGKRKIYEAKIWVRPWMNVNMTQVQEFKLAHDFCQDDLLLDNM