Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27316.t1 | XP_013466098.1 | 94.5 | 457 | 25 | 0 | 1 | 457 | 1 | 457 | 5.60E-245 | 856.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27316.t1 | Q9LH84 | 49.6 | 466 | 221 | 4 | 4 | 465 | 7 | 462 | 9.1e-127 | 455.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27316.t1 | A0A072VE89 | 94.5 | 457 | 25 | 0 | 1 | 457 | 1 | 457 | 4.0e-245 | 856.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene27316 | 0.8016 | 8.34E-49 | -1.69E-46 |
| MS.gene049254 | MS.gene27316 | 0.820676 | 6.05E-53 | -1.69E-46 |
| MS.gene049452 | MS.gene27316 | 0.818863 | 1.57E-52 | -1.69E-46 |
| MS.gene049958 | MS.gene27316 | 0.812525 | 4.06E-51 | -1.69E-46 |
| MS.gene050759 | MS.gene27316 | 0.824431 | 8.10E-54 | -1.69E-46 |
| MS.gene050962 | MS.gene27316 | 0.820175 | 7.88E-53 | -1.69E-46 |
| MS.gene051198 | MS.gene27316 | 0.806239 | 9.06E-50 | -1.69E-46 |
| MS.gene051279 | MS.gene27316 | 0.828259 | 9.93E-55 | -1.69E-46 |
| MS.gene051490 | MS.gene27316 | 0.826627 | 2.45E-54 | -1.69E-46 |
| MS.gene051744 | MS.gene27316 | 0.852343 | 4.91E-61 | -1.69E-46 |
| MS.gene052016 | MS.gene27316 | 0.82779 | 1.29E-54 | -1.69E-46 |
| MS.gene052463 | MS.gene27316 | 0.815146 | 1.07E-51 | -1.69E-46 |
| MS.gene052582 | MS.gene27316 | 0.820614 | 6.25E-53 | -1.69E-46 |
| MS.gene052584 | MS.gene27316 | 0.834635 | 2.68E-56 | -1.69E-46 |
| MS.gene053226 | MS.gene27316 | 0.839481 | 1.54E-57 | -1.69E-46 |
| MS.gene053296 | MS.gene27316 | 0.929519 | 6.19E-93 | -1.69E-46 |
| MS.gene05414 | MS.gene27316 | 0.808749 | 2.66E-50 | -1.69E-46 |
| MS.gene05422 | MS.gene27316 | 0.807736 | 4.37E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene27316 | 0.803639 | 3.17E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene27316 | 0.853696 | 2.01E-61 | -1.69E-46 |
| MS.gene054576 | MS.gene27316 | 0.815879 | 7.37E-52 | -1.69E-46 |
| MS.gene05458 | MS.gene27316 | 0.823268 | 1.52E-53 | -1.69E-46 |
| MS.gene054651 | MS.gene27316 | 0.834445 | 2.99E-56 | -1.69E-46 |
| MS.gene054775 | MS.gene27316 | 0.83518 | 1.95E-56 | -1.69E-46 |
| MS.gene054991 | MS.gene27316 | 0.814053 | 1.87E-51 | -1.69E-46 |
| MS.gene055513 | MS.gene27316 | 0.829752 | 4.32E-55 | -1.69E-46 |
| MS.gene056050 | MS.gene27316 | 0.805502 | 1.29E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene27316 | 0.82241 | 2.40E-53 | -1.69E-46 |
| MS.gene05686 | MS.gene27316 | 0.822366 | 2.46E-53 | -1.69E-46 |
| MS.gene057034 | MS.gene27316 | 0.818743 | 1.67E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene27316 | 0.825879 | 3.68E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene27316 | 0.817515 | 3.17E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene27316 | 0.804482 | 2.12E-49 | -1.69E-46 |
| MS.gene058625 | MS.gene27316 | 0.804934 | 1.70E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene27316 | 0.805913 | 1.06E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene27316 | 0.804318 | 2.29E-49 | -1.69E-46 |
| MS.gene059570 | MS.gene27316 | 0.802625 | 5.13E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene27316 | 0.801041 | 1.09E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27316.t1 | MTR_1g022245 | 96.994 | 499 | 15 | 0 | 1 | 499 | 1 | 499 | 0.0 | 1007 |
| MS.gene27316.t1 | MTR_4g107370 | 53.689 | 488 | 218 | 4 | 1 | 483 | 6 | 490 | 0.0 | 540 |
| MS.gene27316.t1 | MTR_2g030420 | 54.082 | 490 | 178 | 5 | 3 | 477 | 8 | 465 | 0.0 | 539 |
| MS.gene27316.t1 | MTR_1g022250 | 56.144 | 472 | 189 | 5 | 3 | 471 | 5 | 461 | 0.0 | 536 |
| MS.gene27316.t1 | MTR_6g009540 | 51.810 | 525 | 217 | 9 | 3 | 511 | 5 | 509 | 3.40e-180 | 516 |
| MS.gene27316.t1 | MTR_7g008040 | 53.664 | 464 | 206 | 5 | 1 | 460 | 1 | 459 | 4.74e-176 | 507 |
| MS.gene27316.t1 | MTR_6g009520 | 48.462 | 520 | 247 | 6 | 3 | 511 | 7 | 516 | 6.41e-169 | 488 |
| MS.gene27316.t1 | MTR_6g009553 | 53.778 | 450 | 195 | 7 | 21 | 460 | 1 | 447 | 3.71e-165 | 476 |
| MS.gene27316.t1 | MTR_6g009720 | 51.183 | 465 | 217 | 4 | 3 | 460 | 7 | 468 | 2.16e-164 | 476 |
| MS.gene27316.t1 | MTR_6g009770 | 50.644 | 466 | 196 | 8 | 3 | 460 | 7 | 446 | 1.78e-154 | 450 |
| MS.gene27316.t1 | MTR_6g042290 | 47.495 | 459 | 208 | 5 | 7 | 460 | 20 | 450 | 8.73e-148 | 433 |
| MS.gene27316.t1 | MTR_6g009510 | 45.892 | 499 | 181 | 11 | 3 | 489 | 20 | 441 | 4.16e-137 | 404 |
| MS.gene27316.t1 | MTR_6g009660 | 42.857 | 518 | 217 | 11 | 4 | 497 | 5 | 467 | 3.11e-127 | 379 |
| MS.gene27316.t1 | MTR_5g020980 | 37.378 | 511 | 260 | 10 | 4 | 491 | 13 | 486 | 3.82e-108 | 331 |
| MS.gene27316.t1 | MTR_4g128260 | 36.210 | 533 | 295 | 11 | 1 | 506 | 1 | 515 | 1.98e-106 | 328 |
| MS.gene27316.t1 | MTR_2g076970 | 38.645 | 502 | 252 | 14 | 1 | 491 | 1 | 457 | 2.71e-106 | 325 |
| MS.gene27316.t1 | MTR_2g061820 | 38.462 | 455 | 244 | 10 | 8 | 449 | 3 | 434 | 2.78e-103 | 319 |
| MS.gene27316.t1 | MTR_2g076920 | 37.426 | 505 | 263 | 10 | 1 | 496 | 7 | 467 | 2.77e-102 | 315 |
| MS.gene27316.t1 | MTR_2g076690 | 40.416 | 433 | 209 | 8 | 61 | 484 | 65 | 457 | 3.49e-101 | 312 |
| MS.gene27316.t1 | MTR_2g076710 | 37.474 | 475 | 248 | 10 | 1 | 464 | 5 | 441 | 5.35e-99 | 306 |
| MS.gene27316.t1 | MTR_2g076990 | 39.450 | 436 | 217 | 8 | 61 | 487 | 1 | 398 | 7.90e-97 | 300 |
| MS.gene27316.t1 | MTR_2g076840 | 36.820 | 478 | 246 | 10 | 1 | 466 | 1 | 434 | 2.58e-95 | 305 |
| MS.gene27316.t1 | MTR_2g076840 | 45.149 | 268 | 117 | 4 | 195 | 462 | 467 | 704 | 6.67e-72 | 243 |
| MS.gene27316.t1 | MTR_8g076920 | 35.283 | 513 | 276 | 11 | 9 | 508 | 28 | 497 | 5.12e-95 | 297 |
| MS.gene27316.t1 | MTR_2g076900 | 35.800 | 500 | 260 | 12 | 1 | 484 | 1 | 455 | 1.54e-92 | 290 |
| MS.gene27316.t1 | MTR_5g020990 | 37.650 | 417 | 212 | 10 | 63 | 461 | 84 | 470 | 1.56e-87 | 278 |
| MS.gene27316.t1 | MTR_3g103150 | 42.581 | 310 | 139 | 6 | 154 | 461 | 4 | 276 | 3.43e-78 | 247 |
| MS.gene27316.t1 | MTR_1g011680 | 68.675 | 166 | 45 | 3 | 335 | 499 | 7 | 166 | 4.33e-63 | 204 |
| MS.gene27316.t1 | MTR_7g117950 | 30.993 | 413 | 215 | 10 | 57 | 462 | 57 | 406 | 3.38e-60 | 205 |
| MS.gene27316.t1 | MTR_3g092060 | 31.959 | 388 | 203 | 8 | 61 | 441 | 63 | 396 | 7.30e-58 | 200 |
| MS.gene27316.t1 | MTR_5g021020 | 35.276 | 326 | 154 | 8 | 138 | 461 | 135 | 405 | 2.31e-55 | 191 |
| MS.gene27316.t1 | MTR_8g027200 | 28.059 | 474 | 239 | 13 | 35 | 501 | 34 | 412 | 3.54e-48 | 173 |
| MS.gene27316.t1 | MTR_1g113750 | 67.200 | 125 | 22 | 2 | 389 | 509 | 16 | 125 | 9.25e-45 | 154 |
| MS.gene27316.t1 | MTR_5g020720 | 37.363 | 182 | 81 | 6 | 259 | 437 | 25 | 176 | 2.74e-27 | 108 |
| MS.gene27316.t1 | MTR_5g021000 | 50.000 | 108 | 49 | 2 | 356 | 461 | 1 | 105 | 1.03e-26 | 106 |
| MS.gene27316.t1 | MTR_1g008630 | 64.198 | 81 | 8 | 1 | 405 | 485 | 1 | 60 | 2.00e-22 | 92.0 |
| MS.gene27316.t1 | MTR_5g021010 | 48.352 | 91 | 42 | 2 | 373 | 461 | 34 | 121 | 1.35e-18 | 82.0 |
| MS.gene27316.t1 | MTR_2g076820 | 53.846 | 52 | 24 | 0 | 389 | 440 | 1 | 52 | 1.99e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27316.t1 | AT3G28510 | 51.068 | 468 | 215 | 4 | 4 | 467 | 7 | 464 | 9.46e-167 | 482 |
| MS.gene27316.t1 | AT3G28580 | 50.649 | 462 | 213 | 6 | 1 | 457 | 4 | 455 | 2.08e-158 | 460 |
| MS.gene27316.t1 | AT3G28540 | 49.679 | 467 | 215 | 7 | 1 | 459 | 1 | 455 | 5.35e-156 | 454 |
| MS.gene27316.t1 | AT3G28540 | 49.679 | 467 | 215 | 7 | 1 | 459 | 1 | 455 | 6.10e-156 | 454 |
| MS.gene27316.t1 | AT5G40010 | 48.077 | 520 | 246 | 7 | 1 | 511 | 4 | 508 | 2.54e-152 | 445 |
| MS.gene27316.t1 | AT3G28600 | 49.895 | 475 | 190 | 10 | 8 | 465 | 7 | 450 | 5.20e-150 | 438 |
| MS.gene27316.t1 | AT3G28610 | 48.434 | 479 | 194 | 10 | 7 | 465 | 5 | 450 | 2.38e-148 | 433 |
| MS.gene27316.t1 | AT5G40000 | 46.720 | 503 | 205 | 10 | 1 | 481 | 4 | 465 | 1.10e-145 | 426 |
| MS.gene27316.t1 | AT3G28580 | 47.186 | 462 | 207 | 7 | 1 | 457 | 4 | 433 | 1.72e-140 | 414 |
| MS.gene27316.t1 | AT3G28570 | 46.888 | 482 | 201 | 9 | 8 | 476 | 9 | 448 | 2.46e-138 | 407 |
| MS.gene27316.t1 | AT3G28570 | 46.888 | 482 | 201 | 9 | 8 | 476 | 16 | 455 | 2.84e-138 | 407 |
| MS.gene27316.t1 | AT3G28520 | 47.035 | 489 | 223 | 8 | 12 | 480 | 1 | 473 | 1.16e-135 | 401 |
| MS.gene27316.t1 | AT3G28600 | 46.526 | 475 | 167 | 9 | 8 | 465 | 7 | 411 | 1.91e-133 | 394 |
| MS.gene27316.t1 | AT4G25835 | 38.384 | 495 | 272 | 10 | 1 | 483 | 1 | 474 | 1.13e-108 | 333 |
| MS.gene27316.t1 | AT5G57480 | 36.893 | 515 | 289 | 11 | 1 | 502 | 1 | 492 | 6.61e-108 | 331 |
| MS.gene27316.t1 | AT3G50940 | 40.173 | 463 | 224 | 11 | 20 | 471 | 31 | 451 | 1.95e-105 | 323 |
| MS.gene27316.t1 | AT2G18193 | 38.901 | 473 | 236 | 12 | 15 | 477 | 25 | 454 | 1.98e-103 | 319 |
| MS.gene27316.t1 | AT3G50930 | 39.050 | 484 | 252 | 9 | 16 | 485 | 73 | 527 | 8.34e-102 | 317 |
| MS.gene27316.t1 | AT5G17740 | 36.382 | 503 | 272 | 12 | 4 | 494 | 13 | 479 | 1.80e-96 | 302 |
| MS.gene27316.t1 | AT4G30250 | 37.605 | 476 | 265 | 7 | 1 | 461 | 3 | 461 | 4.51e-95 | 298 |
| MS.gene27316.t1 | AT4G30250 | 37.605 | 476 | 265 | 7 | 1 | 461 | 1 | 459 | 4.75e-95 | 298 |
| MS.gene27316.t1 | AT2G18190 | 36.498 | 474 | 246 | 11 | 15 | 476 | 26 | 456 | 2.33e-94 | 295 |
| MS.gene27316.t1 | AT5G17730 | 37.412 | 425 | 217 | 8 | 57 | 468 | 62 | 450 | 1.34e-90 | 285 |
| MS.gene27316.t1 | AT5G17760 | 36.364 | 451 | 227 | 9 | 64 | 490 | 72 | 486 | 1.70e-88 | 281 |
| MS.gene27316.t1 | AT4G05380 | 40.162 | 371 | 186 | 5 | 101 | 468 | 117 | 454 | 2.66e-86 | 273 |
| MS.gene27316.t1 | AT5G17750 | 38.220 | 382 | 187 | 7 | 64 | 427 | 41 | 391 | 5.17e-79 | 253 |
| MS.gene27316.t1 | AT1G43910 | 35.322 | 419 | 235 | 7 | 46 | 464 | 68 | 450 | 3.52e-78 | 253 |
| MS.gene27316.t1 | AT2G46620 | 32.319 | 427 | 224 | 9 | 45 | 462 | 47 | 417 | 3.84e-65 | 219 |
| MS.gene27316.t1 | AT3G29800 | 31.095 | 402 | 207 | 10 | 64 | 458 | 39 | 377 | 2.37e-51 | 181 |
| MS.gene27316.t1 | AT5G17760 | 34.328 | 268 | 148 | 5 | 55 | 305 | 66 | 322 | 2.07e-49 | 173 |
| MS.gene27316.t1 | AT3G28560 | 41.204 | 216 | 112 | 6 | 4 | 212 | 7 | 214 | 9.11e-44 | 155 |
| MS.gene27316.t1 | AT4G05340 | 47.525 | 101 | 44 | 1 | 313 | 413 | 2 | 93 | 2.76e-20 | 86.3 |
Find 82 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAAGTCAGGTAACACTTTC+TGG | 0.123714 | 1.2:-2419075 | MS.gene27316:CDS |
| TTGCAAGCTTAATGTTTATA+TGG | 0.183844 | 1.2:-2420070 | MS.gene27316:CDS |
| ACCAAACATGTTTGAACTTT+GGG | 0.187189 | 1.2:-2420111 | None:intergenic |
| AAGAGAGGCTATTTGCTTTA+TGG | 0.209267 | 1.2:-2419399 | MS.gene27316:CDS |
| CATCTCAGCCTCTTCCTTAG+AGG | 0.245609 | 1.2:+2418720 | None:intergenic |
| TGAATTTCATTGATGGGATT+TGG | 0.272209 | 1.2:-2419047 | MS.gene27316:CDS |
| TTTGAACTTTGGGGTCTAAT+AGG | 0.288966 | 1.2:-2420101 | MS.gene27316:CDS |
| CACCTTGAAGTTTCTTTGCT+TGG | 0.291226 | 1.2:+2419865 | None:intergenic |
| ATCTTACCTTAACCATGTTT+TGG | 0.294915 | 1.2:-2419649 | MS.gene27316:CDS |
| AGGAGATTGGTTTGAGTAAG+AGG | 0.309573 | 1.2:-2419617 | MS.gene27316:CDS |
| AATGAAACAGAATTTGTGTT+AGG | 0.312689 | 1.2:+2419741 | None:intergenic |
| AAAGAGTATTATGCGAAAAT+TGG | 0.315513 | 1.2:-2419432 | MS.gene27316:CDS |
| TTGAAGGGGTTAAGTTTGTT+TGG | 0.318297 | 1.2:-2419782 | MS.gene27316:CDS |
| AACCAAACATGTTTGAACTT+TGG | 0.318708 | 1.2:-2420112 | None:intergenic |
| AATGTATAAGAAAGCTTCAT+TGG | 0.322787 | 1.2:+2419917 | None:intergenic |
| CCTTAGAGGTTCTAAGTGCT+TGG | 0.356753 | 1.2:+2418734 | None:intergenic |
| GATTGTTCGCTTGATCTTAC+CGG | 0.367967 | 1.2:-2419216 | MS.gene27316:CDS |
| CTTTATGGTCCTCCTGGAAC+AGG | 0.377541 | 1.2:-2419384 | MS.gene27316:CDS |
| ATCCTTTGTTCGAGATGGTT+CGG | 0.385486 | 1.2:-2418858 | MS.gene27316:CDS |
| CCAAGCACTTAGAACCTCTA+AGG | 0.388467 | 1.2:-2418734 | MS.gene27316:CDS |
| AAGAATCTAACCTCAACAAA+AGG | 0.394204 | 1.2:+2419966 | None:intergenic |
| TGCAAGCTTAATGTTTATAT+GGG | 0.401015 | 1.2:-2420069 | MS.gene27316:CDS |
| TTGAAGTTTCTTTGCTTGGT+TGG | 0.404145 | 1.2:+2419869 | None:intergenic |
| TTGGAACAAGGTAAGGAGAT+TGG | 0.405159 | 1.2:-2419630 | MS.gene27316:CDS |
| TACACAAATTGCACAGGTAA+TGG | 0.421924 | 1.2:-2419582 | MS.gene27316:CDS |
| GGGTTATTGAATTTCATTGA+TGG | 0.427942 | 1.2:-2419054 | MS.gene27316:CDS |
| AGCAAAGAACAAAGCAAGTC+AGG | 0.436198 | 1.2:-2419088 | MS.gene27316:CDS |
| CAAGTCAGGTAACACTTTCT+GGG | 0.437874 | 1.2:-2419074 | MS.gene27316:CDS |
| CGAGATGGTTCGGTCCTTGT+TGG | 0.450554 | 1.2:-2418848 | MS.gene27316:CDS |
| ATCTCATCCTTTGTTCGAGA+TGG | 0.457463 | 1.2:-2418863 | MS.gene27316:CDS |
| AACAAAAGGGTAACAAAAGT+TGG | 0.459264 | 1.2:+2419980 | None:intergenic |
| ATGCGAAAATTGGTAAGCCT+TGG | 0.466070 | 1.2:-2419422 | MS.gene27316:CDS |
| ATGAAACAGAATTTGTGTTA+GGG | 0.466214 | 1.2:+2419742 | None:intergenic |
| AAAGTCTTTAGTTCTAAAGA+TGG | 0.468242 | 1.2:-2419832 | MS.gene27316:CDS |
| TATTTGCTTTATGGTCCTCC+TGG | 0.473621 | 1.2:-2419390 | MS.gene27316:CDS |
| AACCAAGCAAAGAAACTTCA+AGG | 0.474512 | 1.2:-2419867 | MS.gene27316:CDS |
| CCTTACCTTGTTCCAAAACA+TGG | 0.476787 | 1.2:+2419637 | None:intergenic |
| CATATTGAGTTGTCTTATTG+TGG | 0.480153 | 1.2:-2418931 | MS.gene27316:CDS |
| GGTTATTGAATTTCATTGAT+GGG | 0.481156 | 1.2:-2419053 | MS.gene27316:CDS |
| GTTGCTGCTGCTTCTCTGAA+GGG | 0.484248 | 1.2:-2419129 | MS.gene27316:CDS |
| CTTGTTCCAAAACATGGTTA+AGG | 0.486598 | 1.2:+2419643 | None:intergenic |
| TGATCTTGTTACATTCAGTA+AGG | 0.490186 | 1.2:-2419457 | MS.gene27316:CDS |
| GGGATTTGGTCTGCTAGTAC+TGG | 0.502830 | 1.2:-2419033 | MS.gene27316:CDS |
| AGAAGAAGGGACAGGTGAAG+AGG | 0.504002 | 1.2:-2418662 | MS.gene27316:CDS |
| TAAGAAAGAAGCAGAGATGA+AGG | 0.504395 | 1.2:-2418692 | MS.gene27316:CDS |
| GAATGCCAATGAAAATTCCT+AGG | 0.507426 | 1.2:-2418587 | None:intergenic |
| GCAATTGTCTCAAAAGAAGA+TGG | 0.507729 | 1.2:+2419513 | None:intergenic |
| AGTTGCTGCTGCTTCTCTGA+AGG | 0.508302 | 1.2:-2419130 | MS.gene27316:CDS |
| GTTAGATTCTTTGAGAACAT+TGG | 0.509702 | 1.2:-2419954 | MS.gene27316:CDS |
| CAGGTAATGGTGAATATGAA+AGG | 0.510294 | 1.2:-2419569 | MS.gene27316:CDS |
| ATGAAGGCTGAGAAAGAAGA+AGG | 0.511615 | 1.2:-2418676 | MS.gene27316:CDS |
| CGGTCAGAGGAAGACAGATT+CGG | 0.513344 | 1.2:-2419196 | MS.gene27316:CDS |
| TCATTGGCATTCTCTTCCTT+AGG | 0.514941 | 1.2:+2418598 | None:intergenic |
| CCAAACATGTTTGAACTTTG+GGG | 0.515294 | 1.2:-2420110 | None:intergenic |
| AGCAAATAGCCTCTCTTCCA+AGG | 0.530468 | 1.2:+2419405 | None:intergenic |
| TTTGATTCGTCGTGGACGAA+TGG | 0.533266 | 1.2:-2418959 | MS.gene27316:CDS |
| AGGTAATGGTGAATATGAAA+GGG | 0.535037 | 1.2:-2419568 | MS.gene27316:CDS |
| AGAATCTAACCTCAACAAAA+GGG | 0.545291 | 1.2:+2419967 | None:intergenic |
| CGCTTGATCTTACCGGTCAG+AGG | 0.554416 | 1.2:-2419209 | MS.gene27316:CDS |
| GACCGAACCATCTCGAACAA+AGG | 0.555408 | 1.2:+2418856 | None:intergenic |
| TGAAGAGGATTCATCAGAGA+AGG | 0.557487 | 1.2:-2418647 | MS.gene27316:CDS |
| CGAATCTGTCTTCCTCTGAC+CGG | 0.558778 | 1.2:+2419197 | None:intergenic |
| ATTGGTAAGCCTTGGAAGAG+AGG | 0.563864 | 1.2:-2419414 | MS.gene27316:CDS |
| ACTTAGAACCTCTAAGGAAG+AGG | 0.569363 | 1.2:-2418728 | MS.gene27316:CDS |
| GCTGAGAAAGAAGAAGGGAC+AGG | 0.571855 | 1.2:-2418670 | MS.gene27316:CDS |
| AAGCTTTACACAAATTGCAC+AGG | 0.572705 | 1.2:-2419588 | MS.gene27316:CDS |
| ACATCTTCATCAGCTACCTT+AGG | 0.574849 | 1.2:+2418781 | None:intergenic |
| TATATTCACTACTAACTATG+TGG | 0.586952 | 1.2:-2418998 | MS.gene27316:CDS |
| TGCTGAAAACTTGATGCCTA+AGG | 0.589054 | 1.2:-2418797 | MS.gene27316:CDS |
| AATATGAAAGGGGAGGTAAG+TGG | 0.596088 | 1.2:-2419557 | MS.gene27316:CDS |
| TGCAGAAGACAAGAAGCCTA+AGG | 0.596943 | 1.2:-2418614 | MS.gene27316:CDS |
| AGAGGATTCATCAGAGAAGG+AGG | 0.600261 | 1.2:-2418644 | MS.gene27316:CDS |
| GGTAGCTGATGAAGATGTTG+AGG | 0.602813 | 1.2:-2418776 | MS.gene27316:CDS |
| CATGTTAGTCTCTTCCAACA+AGG | 0.626039 | 1.2:+2418834 | None:intergenic |
| TGAAGGCTGAGAAAGAAGAA+GGG | 0.627565 | 1.2:-2418675 | MS.gene27316:CDS |
| AATCACGAGTAAATCTGTTG+TGG | 0.629066 | 1.2:-2419253 | MS.gene27316:CDS |
| GATCAAGCTTTGATTCGTCG+TGG | 0.629566 | 1.2:-2418967 | MS.gene27316:CDS |
| ATGTTGAGGCATCATTAGAG+AGG | 0.635432 | 1.2:-2418762 | MS.gene27316:CDS |
| AATGGTGAATATGAAAGGGG+AGG | 0.665295 | 1.2:-2419564 | MS.gene27316:CDS |
| GGTAATGGTGAATATGAAAG+GGG | 0.666341 | 1.2:-2419567 | MS.gene27316:CDS |
| ATGAGTGCATGAACTTGATG+AGG | 0.669050 | 1.2:+2420026 | None:intergenic |
| GACTGCGATGAAATGTGAGA+AGG | 0.695268 | 1.2:+2419691 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGAAACAGAATTTGTGTT+AGG | + | chr1.2:2418954-2418973 | None:intergenic | 25.0% |
| ! | AATGTATAAGAAAGCTTCAT+TGG | + | chr1.2:2418778-2418797 | None:intergenic | 25.0% |
| ! | ATGAAACAGAATTTGTGTTA+GGG | + | chr1.2:2418953-2418972 | None:intergenic | 25.0% |
| ! | GGTTATTGAATTTCATTGAT+GGG | - | chr1.2:2419639-2419658 | MS.gene27316:CDS | 25.0% |
| ! | TATATTCACTACTAACTATG+TGG | - | chr1.2:2419694-2419713 | MS.gene27316:CDS | 25.0% |
| ! | TGAAAAATAATGCTGAGTTA+AGG | - | chr1.2:2419402-2419421 | MS.gene27316:CDS | 25.0% |
| ! | TGCAAGCTTAATGTTTATAT+GGG | - | chr1.2:2418623-2418642 | MS.gene27316:CDS | 25.0% |
| ! | TTGCAAGCTTAATGTTTATA+TGG | - | chr1.2:2418622-2418641 | MS.gene27316:CDS | 25.0% |
| !! | ACAATCTCTTTCTTCTTTTT+AGG | + | chr1.2:2419212-2419231 | None:intergenic | 25.0% |
| !!! | AAAGAGTATTATGCGAAAAT+TGG | - | chr1.2:2419260-2419279 | MS.gene27316:CDS | 25.0% |
| !!! | AAAGTCTTTAGTTCTAAAGA+TGG | - | chr1.2:2418860-2418879 | MS.gene27316:CDS | 25.0% |
| !!! | AAGTTTTATGATGAGTTTGA+AGG | - | chr1.2:2418894-2418913 | MS.gene27316:CDS | 25.0% |
| !!! | AGTTTTATGATGAGTTTGAA+GGG | - | chr1.2:2418895-2418914 | MS.gene27316:CDS | 25.0% |
| AACAAAAGGGTAACAAAAGT+TGG | + | chr1.2:2418715-2418734 | None:intergenic | 30.0% | |
| AAGAATCTAACCTCAACAAA+AGG | + | chr1.2:2418729-2418748 | None:intergenic | 30.0% | |
| AGAAAAAGAGGACAAAAATG+AGG | - | chr1.2:2419529-2419548 | MS.gene27316:CDS | 30.0% | |
| AGAATCTAACCTCAACAAAA+GGG | + | chr1.2:2418728-2418747 | None:intergenic | 30.0% | |
| GGGTTATTGAATTTCATTGA+TGG | - | chr1.2:2419638-2419657 | MS.gene27316:CDS | 30.0% | |
| TGAATTTCATTGATGGGATT+TGG | - | chr1.2:2419645-2419664 | MS.gene27316:CDS | 30.0% | |
| TGATCTTGTTACATTCAGTA+AGG | - | chr1.2:2419235-2419254 | MS.gene27316:CDS | 30.0% | |
| ! | AGGTAATGGTGAATATGAAA+GGG | - | chr1.2:2419124-2419143 | MS.gene27316:CDS | 30.0% |
| ! | ATCTTACCTTAACCATGTTT+TGG | - | chr1.2:2419043-2419062 | MS.gene27316:CDS | 30.0% |
| ! | CATATTGAGTTGTCTTATTG+TGG | - | chr1.2:2419761-2419780 | MS.gene27316:CDS | 30.0% |
| ! | GAGTTAAGGAAACTTTTGAT+TGG | - | chr1.2:2419416-2419435 | MS.gene27316:CDS | 30.0% |
| ! | GTTAGATTCTTTGAGAACAT+TGG | - | chr1.2:2418738-2418757 | MS.gene27316:CDS | 30.0% |
| ! | GTTTTATGATGAGTTTGAAG+GGG | - | chr1.2:2418896-2418915 | MS.gene27316:CDS | 30.0% |
| !! | CTCAGCATTATTTTTCACAT+TGG | + | chr1.2:2419399-2419418 | None:intergenic | 30.0% |
| !!! | TTGTCTTATTGTGGTTTTGA+TGG | - | chr1.2:2419770-2419789 | MS.gene27316:CDS | 30.0% |
| !!! | TTTTGATGGATTCAAAATGC+TGG | - | chr1.2:2419784-2419803 | MS.gene27316:CDS | 30.0% |
| AAAAGAGGACAAAAATGAGG+AGG | - | chr1.2:2419532-2419551 | MS.gene27316:CDS | 35.0% | |
| AACCAAGCAAAGAAACTTCA+AGG | - | chr1.2:2418825-2418844 | MS.gene27316:CDS | 35.0% | |
| AAGAGAGGCTATTTGCTTTA+TGG | - | chr1.2:2419293-2419312 | MS.gene27316:CDS | 35.0% | |
| AAGCTTTACACAAATTGCAC+AGG | - | chr1.2:2419104-2419123 | MS.gene27316:CDS | 35.0% | |
| AATCACGAGTAAATCTGTTG+TGG | - | chr1.2:2419439-2419458 | MS.gene27316:CDS | 35.0% | |
| CTTGTTCCAAAACATGGTTA+AGG | + | chr1.2:2419052-2419071 | None:intergenic | 35.0% | |
| GCAATTGTCTCAAAAGAAGA+TGG | + | chr1.2:2419182-2419201 | None:intergenic | 35.0% | |
| TAAGAAAGAAGCAGAGATGA+AGG | - | chr1.2:2420000-2420019 | MS.gene27316:CDS | 35.0% | |
| TACACAAATTGCACAGGTAA+TGG | - | chr1.2:2419110-2419129 | MS.gene27316:CDS | 35.0% | |
| TTGAAGTTTCTTTGCTTGGT+TGG | + | chr1.2:2418826-2418845 | None:intergenic | 35.0% | |
| ! | CAGGTAATGGTGAATATGAA+AGG | - | chr1.2:2419123-2419142 | MS.gene27316:CDS | 35.0% |
| ! | CTTTTGTTACCCTTTTGTTG+AGG | - | chr1.2:2418716-2418735 | MS.gene27316:CDS | 35.0% |
| ! | GGTAATGGTGAATATGAAAG+GGG | - | chr1.2:2419125-2419144 | MS.gene27316:CDS | 35.0% |
| ! | TTGAAGGGGTTAAGTTTGTT+TGG | - | chr1.2:2418910-2418929 | MS.gene27316:CDS | 35.0% |
| !! | TAGAATCTTTTGTCTGAAGC+TGG | + | chr1.2:2418981-2419000 | None:intergenic | 35.0% |
| !! | TTTGAACTTTGGGGTCTAAT+AGG | - | chr1.2:2418591-2418610 | MS.gene27316:CDS | 35.0% |
| !!! | CTTAACCATGTTTTGGAACA+AGG | - | chr1.2:2419050-2419069 | MS.gene27316:CDS | 35.0% |
| AATATGAAAGGGGAGGTAAG+TGG | - | chr1.2:2419135-2419154 | MS.gene27316:CDS | 40.0% | |
| AATGGTGAATATGAAAGGGG+AGG | - | chr1.2:2419128-2419147 | MS.gene27316:CDS | 40.0% | |
| ACATCTTCATCAGCTACCTT+AGG | + | chr1.2:2419914-2419933 | None:intergenic | 40.0% | |
| AGCAAAGAACAAAGCAAGTC+AGG | - | chr1.2:2419604-2419623 | MS.gene27316:CDS | 40.0% | |
| ATCCTTTGTTCGAGATGGTT+CGG | - | chr1.2:2419834-2419853 | MS.gene27316:CDS | 40.0% | |
| ATCTCATCCTTTGTTCGAGA+TGG | - | chr1.2:2419829-2419848 | MS.gene27316:CDS | 40.0% | |
| ATGAAGGCTGAGAAAGAAGA+AGG | - | chr1.2:2420016-2420035 | MS.gene27316:CDS | 40.0% | |
| ATGAGTGCATGAACTTGATG+AGG | + | chr1.2:2418669-2418688 | None:intergenic | 40.0% | |
| ATGCGAAAATTGGTAAGCCT+TGG | - | chr1.2:2419270-2419289 | MS.gene27316:CDS | 40.0% | |
| CAAGTCAGGTAACACTTTCT+GGG | - | chr1.2:2419618-2419637 | MS.gene27316:CDS | 40.0% | |
| CACCTTGAAGTTTCTTTGCT+TGG | + | chr1.2:2418830-2418849 | None:intergenic | 40.0% | |
| CCTTACCTTGTTCCAAAACA+TGG | + | chr1.2:2419058-2419077 | None:intergenic | 40.0% | |
| GATTGTTCGCTTGATCTTAC+CGG | - | chr1.2:2419476-2419495 | MS.gene27316:CDS | 40.0% | |
| GGAGAATGACAGAGAAAAAG+AGG | - | chr1.2:2419517-2419536 | MS.gene27316:CDS | 40.0% | |
| TATTTGCTTTATGGTCCTCC+TGG | - | chr1.2:2419302-2419321 | MS.gene27316:CDS | 40.0% | |
| TCATTGGCATTCTCTTCCTT+AGG | + | chr1.2:2420097-2420116 | None:intergenic | 40.0% | |
| TGAAGAGGATTCATCAGAGA+AGG | - | chr1.2:2420045-2420064 | MS.gene27316:CDS | 40.0% | |
| TGAAGGCTGAGAAAGAAGAA+GGG | - | chr1.2:2420017-2420036 | MS.gene27316:CDS | 40.0% | |
| TGCTGAAAACTTGATGCCTA+AGG | - | chr1.2:2419895-2419914 | MS.gene27316:CDS | 40.0% | |
| TTGGAACAAGGTAAGGAGAT+TGG | - | chr1.2:2419062-2419081 | MS.gene27316:CDS | 40.0% | |
| ! | AAGTTTTCAGCAACATCAGC+TGG | + | chr1.2:2419887-2419906 | None:intergenic | 40.0% |
| ! | ACTTAGAACCTCTAAGGAAG+AGG | - | chr1.2:2419964-2419983 | MS.gene27316:CDS | 40.0% |
| ! | AGTGATGATTTTCCTGTTCC+AGG | + | chr1.2:2419323-2419342 | None:intergenic | 40.0% |
| ! | ATGTTGAGGCATCATTAGAG+AGG | - | chr1.2:2419930-2419949 | MS.gene27316:CDS | 40.0% |
| ! | CATGTTAGTCTCTTCCAACA+AGG | + | chr1.2:2419861-2419880 | None:intergenic | 40.0% |
| !! | AGGAGATTGGTTTGAGTAAG+AGG | - | chr1.2:2419075-2419094 | MS.gene27316:CDS | 40.0% |
| !!! | CCATGTTTTGGAACAAGGTA+AGG | - | chr1.2:2419055-2419074 | MS.gene27316:CDS | 40.0% |
| AGAGGATTCATCAGAGAAGG+AGG | - | chr1.2:2420048-2420067 | MS.gene27316:CDS | 45.0% | |
| AGCAAATAGCCTCTCTTCCA+AGG | + | chr1.2:2419290-2419309 | None:intergenic | 45.0% | |
| ATTGGTAAGCCTTGGAAGAG+AGG | - | chr1.2:2419278-2419297 | MS.gene27316:CDS | 45.0% | |
| CCAAGCACTTAGAACCTCTA+AGG | - | chr1.2:2419958-2419977 | MS.gene27316:CDS | 45.0% | |
| GACTGCGATGAAATGTGAGA+AGG | + | chr1.2:2419004-2419023 | None:intergenic | 45.0% | |
| GCAAGTCAGGTAACACTTTC+TGG | - | chr1.2:2419617-2419636 | MS.gene27316:CDS | 45.0% | |
| GGTAGCTGATGAAGATGTTG+AGG | - | chr1.2:2419916-2419935 | MS.gene27316:CDS | 45.0% | |
| TGCAGAAGACAAGAAGCCTA+AGG | - | chr1.2:2420078-2420097 | MS.gene27316:CDS | 45.0% | |
| ! | GATCAAGCTTTGATTCGTCG+TGG | - | chr1.2:2419725-2419744 | MS.gene27316:CDS | 45.0% |
| ! | GATGATTTTCCTGTTCCAGG+AGG | + | chr1.2:2419320-2419339 | None:intergenic | 45.0% |
| ! | TTTGATTCGTCGTGGACGAA+TGG | - | chr1.2:2419733-2419752 | MS.gene27316:CDS | 45.0% |
| !! | CCTTAGAGGTTCTAAGTGCT+TGG | + | chr1.2:2419961-2419980 | None:intergenic | 45.0% |
| AGAAGAAGGGACAGGTGAAG+AGG | - | chr1.2:2420030-2420049 | MS.gene27316:CDS | 50.0% | |
| CATCTCAGCCTCTTCCTTAG+AGG | + | chr1.2:2419975-2419994 | None:intergenic | 50.0% | |
| CGAATCTGTCTTCCTCTGAC+CGG | + | chr1.2:2419498-2419517 | None:intergenic | 50.0% | |
| CGGTCAGAGGAAGACAGATT+CGG | - | chr1.2:2419496-2419515 | MS.gene27316:CDS | 50.0% | |
| CTTTATGGTCCTCCTGGAAC+AGG | - | chr1.2:2419308-2419327 | MS.gene27316:CDS | 50.0% | |
| GACCGAACCATCTCGAACAA+AGG | + | chr1.2:2419839-2419858 | None:intergenic | 50.0% | |
| GCTGAGAAAGAAGAAGGGAC+AGG | - | chr1.2:2420022-2420041 | MS.gene27316:CDS | 50.0% | |
| ! | AGTTGCTGCTGCTTCTCTGA+AGG | - | chr1.2:2419562-2419581 | MS.gene27316:CDS | 50.0% |
| ! | GGGATTTGGTCTGCTAGTAC+TGG | - | chr1.2:2419659-2419678 | MS.gene27316:CDS | 50.0% |
| ! | GTTGCTGCTGCTTCTCTGAA+GGG | - | chr1.2:2419563-2419582 | MS.gene27316:CDS | 50.0% |
| CGCTTGATCTTACCGGTCAG+AGG | - | chr1.2:2419483-2419502 | MS.gene27316:CDS | 55.0% | |
| !! | CGAGATGGTTCGGTCCTTGT+TGG | - | chr1.2:2419844-2419863 | MS.gene27316:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 2418588 | 2420126 | 2418588 | ID=MS.gene27316 |
| chr1.2 | mRNA | 2418588 | 2420126 | 2418588 | ID=MS.gene27316.t1;Parent=MS.gene27316 |
| chr1.2 | exon | 2418588 | 2420126 | 2418588 | ID=MS.gene27316.t1.exon1;Parent=MS.gene27316.t1 |
| chr1.2 | CDS | 2418588 | 2420126 | 2418588 | ID=cds.MS.gene27316.t1;Parent=MS.gene27316.t1 |
| Gene Sequence |
| Protein sequence |
