Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27381.t1 | XP_024641737.1 | 78 | 246 | 32 | 5 | 1 | 225 | 518 | 762 | 1.50E-93 | 352.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27381.t1 | Q9LD37 | 51.4 | 249 | 81 | 4 | 1 | 220 | 525 | 762 | 5.5e-60 | 232.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27381.t1 | A0A396HMA0 | 78.0 | 246 | 32 | 5 | 1 | 225 | 518 | 762 | 1.1e-93 | 352.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene27381 | 0.818729 | 1.68E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene27381 | 0.821421 | 4.08E-53 | -1.69E-46 |
| MS.gene049669 | MS.gene27381 | 0.820141 | 8.02E-53 | -1.69E-46 |
| MS.gene050228 | MS.gene27381 | 0.800342 | 1.51E-48 | -1.69E-46 |
| MS.gene050355 | MS.gene27381 | 0.800325 | 1.52E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene27381 | 0.806461 | 8.13E-50 | -1.69E-46 |
| MS.gene050780 | MS.gene27381 | 0.805269 | 1.45E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene27381 | 0.807699 | 4.45E-50 | -1.69E-46 |
| MS.gene051452 | MS.gene27381 | 0.803731 | 3.03E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene27381 | 0.833683 | 4.63E-56 | -1.69E-46 |
| MS.gene052333 | MS.gene27381 | 0.820203 | 7.77E-53 | -1.69E-46 |
| MS.gene052389 | MS.gene27381 | 0.80145 | 8.95E-49 | -1.69E-46 |
| MS.gene052464 | MS.gene27381 | 0.830395 | 3.01E-55 | -1.69E-46 |
| MS.gene052685 | MS.gene27381 | 0.802347 | 5.86E-49 | -1.69E-46 |
| MS.gene053006 | MS.gene27381 | 0.830544 | 2.77E-55 | -1.69E-46 |
| MS.gene053963 | MS.gene27381 | 0.819957 | 8.84E-53 | -1.69E-46 |
| MS.gene054093 | MS.gene27381 | 0.805337 | 1.40E-49 | -1.69E-46 |
| MS.gene05410 | MS.gene27381 | 0.810176 | 1.31E-50 | -1.69E-46 |
| MS.gene05414 | MS.gene27381 | 0.81615 | 6.42E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene27381 | 0.812083 | 5.07E-51 | -1.69E-46 |
| MS.gene054405 | MS.gene27381 | 0.800905 | 1.16E-48 | -1.69E-46 |
| MS.gene054479 | MS.gene27381 | 0.802433 | 5.62E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene27381 | 0.809897 | 1.51E-50 | -1.69E-46 |
| MS.gene055225 | MS.gene27381 | 0.817579 | 3.06E-52 | -1.69E-46 |
| MS.gene055701 | MS.gene27381 | 0.836252 | 1.04E-56 | -1.69E-46 |
| MS.gene056087 | MS.gene27381 | 0.834009 | 3.84E-56 | -1.69E-46 |
| MS.gene056506 | MS.gene27381 | 0.811605 | 6.44E-51 | -1.69E-46 |
| MS.gene056779 | MS.gene27381 | 0.82516 | 5.46E-54 | -1.69E-46 |
| MS.gene057079 | MS.gene27381 | 0.812251 | 4.66E-51 | -1.69E-46 |
| MS.gene057194 | MS.gene27381 | 0.83167 | 1.46E-55 | -1.69E-46 |
| MS.gene05761 | MS.gene27381 | 0.844265 | 8.41E-59 | -1.69E-46 |
| MS.gene060840 | MS.gene27381 | 0.800486 | 1.41E-48 | -1.69E-46 |
| MS.gene060879 | MS.gene27381 | 0.812819 | 3.50E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene27381 | -0.825832 | 3.78E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27381.t1 | MTR_5g007630 | 73.469 | 245 | 43 | 5 | 1 | 224 | 498 | 741 | 5.66e-111 | 335 |
| MS.gene27381.t1 | MTR_6g075440 | 62.550 | 251 | 56 | 2 | 1 | 222 | 503 | 744 | 3.52e-98 | 302 |
| MS.gene27381.t1 | MTR_6g075440 | 62.550 | 251 | 56 | 2 | 1 | 222 | 503 | 744 | 6.16e-98 | 301 |
| MS.gene27381.t1 | MTR_6g076060 | 65.432 | 243 | 64 | 4 | 1 | 223 | 231 | 473 | 1.41e-91 | 278 |
| MS.gene27381.t1 | MTR_6g477760 | 58.750 | 240 | 69 | 4 | 1 | 216 | 464 | 697 | 1.46e-80 | 254 |
| MS.gene27381.t1 | MTR_6g075290 | 58.943 | 246 | 71 | 4 | 1 | 222 | 327 | 566 | 8.56e-80 | 249 |
| MS.gene27381.t1 | MTR_6g075290 | 58.943 | 246 | 71 | 4 | 1 | 222 | 488 | 727 | 2.53e-78 | 249 |
| MS.gene27381.t1 | MTR_6g075290 | 58.943 | 246 | 71 | 4 | 1 | 222 | 529 | 768 | 6.94e-78 | 249 |
| MS.gene27381.t1 | MTR_6g075410 | 56.735 | 245 | 65 | 5 | 1 | 216 | 432 | 664 | 4.88e-77 | 244 |
| MS.gene27381.t1 | MTR_6g075390 | 78.912 | 147 | 31 | 0 | 4 | 150 | 449 | 595 | 3.43e-75 | 240 |
| MS.gene27381.t1 | MTR_3g088875 | 36.771 | 223 | 123 | 5 | 3 | 209 | 379 | 599 | 6.23e-35 | 131 |
| MS.gene27381.t1 | MTR_2g460590 | 37.168 | 226 | 124 | 5 | 2 | 211 | 386 | 609 | 1.09e-34 | 130 |
| MS.gene27381.t1 | MTR_3g109030 | 35.565 | 239 | 136 | 6 | 2 | 224 | 416 | 652 | 1.36e-34 | 130 |
| MS.gene27381.t1 | MTR_4g130820 | 36.245 | 229 | 127 | 5 | 1 | 212 | 437 | 663 | 3.72e-34 | 129 |
| MS.gene27381.t1 | MTR_8g027755 | 36.123 | 227 | 125 | 7 | 1 | 209 | 424 | 648 | 9.04e-34 | 128 |
| MS.gene27381.t1 | MTR_7g117310 | 36.207 | 232 | 126 | 8 | 1 | 213 | 420 | 648 | 1.03e-31 | 122 |
| MS.gene27381.t1 | MTR_7g117310 | 36.207 | 232 | 126 | 8 | 1 | 213 | 366 | 594 | 1.07e-31 | 122 |
| MS.gene27381.t1 | MTR_7g117310 | 36.207 | 232 | 126 | 8 | 1 | 213 | 432 | 660 | 1.20e-31 | 122 |
| MS.gene27381.t1 | MTR_3g091080 | 37.004 | 227 | 124 | 6 | 1 | 210 | 415 | 639 | 2.73e-31 | 120 |
| MS.gene27381.t1 | MTR_1g028370 | 34.783 | 230 | 131 | 6 | 1 | 213 | 417 | 644 | 3.02e-31 | 120 |
| MS.gene27381.t1 | MTR_7g034605 | 35.426 | 223 | 126 | 6 | 3 | 209 | 387 | 607 | 2.60e-30 | 118 |
| MS.gene27381.t1 | MTR_1g064240 | 35.294 | 238 | 117 | 7 | 2 | 214 | 406 | 631 | 1.51e-29 | 115 |
| MS.gene27381.t1 | MTR_4g058730 | 35.294 | 221 | 111 | 6 | 7 | 204 | 399 | 610 | 1.48e-28 | 112 |
| MS.gene27381.t1 | MTR_2g094860 | 36.486 | 222 | 117 | 7 | 7 | 209 | 417 | 633 | 1.77e-28 | 112 |
| MS.gene27381.t1 | MTR_8g464570 | 35.398 | 226 | 129 | 6 | 1 | 211 | 406 | 629 | 1.98e-28 | 112 |
| MS.gene27381.t1 | MTR_2g081110 | 33.333 | 231 | 137 | 6 | 1 | 216 | 402 | 630 | 2.09e-28 | 112 |
| MS.gene27381.t1 | MTR_7g012260 | 32.018 | 228 | 130 | 7 | 1 | 209 | 454 | 675 | 2.18e-23 | 98.2 |
| MS.gene27381.t1 | MTR_6g075460 | 82.051 | 39 | 7 | 0 | 185 | 223 | 87 | 125 | 1.58e-14 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27381.t1 | AT3G17690 | 50.607 | 247 | 82 | 4 | 1 | 219 | 492 | 726 | 1.63e-71 | 231 |
| MS.gene27381.t1 | AT3G17700 | 51.200 | 250 | 82 | 4 | 1 | 221 | 525 | 763 | 2.57e-66 | 218 |
| MS.gene27381.t1 | AT3G17700 | 65.217 | 161 | 54 | 1 | 1 | 161 | 525 | 683 | 1.90e-65 | 214 |
| MS.gene27381.t1 | AT3G17690 | 65.132 | 152 | 49 | 2 | 1 | 152 | 492 | 639 | 2.84e-60 | 200 |
| MS.gene27381.t1 | AT4G30360 | 37.662 | 231 | 126 | 4 | 2 | 216 | 413 | 641 | 7.06e-38 | 139 |
| MS.gene27381.t1 | AT4G30560 | 38.938 | 226 | 119 | 5 | 1 | 209 | 444 | 667 | 2.73e-35 | 132 |
| MS.gene27381.t1 | AT4G30560 | 38.938 | 226 | 119 | 5 | 1 | 209 | 444 | 667 | 2.73e-35 | 132 |
| MS.gene27381.t1 | AT4G30560 | 38.938 | 226 | 119 | 5 | 1 | 209 | 418 | 641 | 2.74e-35 | 132 |
| MS.gene27381.t1 | AT4G30560 | 38.938 | 226 | 119 | 5 | 1 | 209 | 418 | 641 | 2.74e-35 | 132 |
| MS.gene27381.t1 | AT4G30560 | 38.938 | 226 | 119 | 5 | 1 | 209 | 418 | 641 | 2.74e-35 | 132 |
| MS.gene27381.t1 | AT2G24610 | 35.294 | 238 | 135 | 5 | 2 | 222 | 413 | 648 | 5.59e-35 | 131 |
| MS.gene27381.t1 | AT2G24610 | 35.294 | 238 | 135 | 5 | 2 | 222 | 428 | 663 | 7.25e-35 | 131 |
| MS.gene27381.t1 | AT1G19780 | 35.556 | 225 | 127 | 4 | 1 | 209 | 228 | 450 | 8.25e-35 | 130 |
| MS.gene27381.t1 | AT1G19780 | 35.556 | 225 | 127 | 4 | 1 | 209 | 439 | 661 | 2.12e-34 | 130 |
| MS.gene27381.t1 | AT1G15990 | 35.111 | 225 | 128 | 4 | 1 | 209 | 433 | 655 | 9.52e-34 | 128 |
| MS.gene27381.t1 | AT2G23980 | 37.333 | 225 | 123 | 4 | 1 | 209 | 418 | 640 | 1.41e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 445 | 598 | 1.45e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 445 | 598 | 1.45e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 445 | 598 | 1.45e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 445 | 598 | 1.45e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 445 | 598 | 1.45e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 468 | 621 | 1.60e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 468 | 621 | 1.60e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 468 | 621 | 1.60e-33 | 127 |
| MS.gene27381.t1 | AT2G23980 | 45.513 | 156 | 76 | 3 | 1 | 149 | 468 | 621 | 1.60e-33 | 127 |
| MS.gene27381.t1 | AT1G01340 | 35.146 | 239 | 118 | 8 | 1 | 213 | 404 | 631 | 5.28e-33 | 125 |
| MS.gene27381.t1 | AT1G01340 | 35.146 | 239 | 118 | 8 | 1 | 213 | 399 | 626 | 5.29e-33 | 125 |
| MS.gene27381.t1 | AT5G57940 | 44.156 | 154 | 77 | 3 | 1 | 147 | 429 | 580 | 8.01e-33 | 125 |
| MS.gene27381.t1 | AT5G57940 | 44.156 | 154 | 77 | 3 | 1 | 147 | 429 | 580 | 8.01e-33 | 125 |
| MS.gene27381.t1 | AT5G57940 | 44.156 | 154 | 77 | 3 | 1 | 147 | 422 | 573 | 8.32e-33 | 125 |
| MS.gene27381.t1 | AT5G53130 | 35.455 | 220 | 124 | 4 | 1 | 204 | 417 | 634 | 1.67e-32 | 124 |
| MS.gene27381.t1 | AT4G01010 | 33.051 | 236 | 139 | 6 | 1 | 219 | 405 | 638 | 2.58e-32 | 124 |
| MS.gene27381.t1 | AT4G01010 | 33.051 | 236 | 139 | 6 | 1 | 219 | 405 | 638 | 2.58e-32 | 124 |
| MS.gene27381.t1 | AT3G48010 | 34.413 | 247 | 103 | 7 | 2 | 211 | 389 | 613 | 3.90e-31 | 120 |
| MS.gene27381.t1 | AT2G28260 | 37.445 | 227 | 118 | 6 | 2 | 209 | 403 | 624 | 8.37e-31 | 119 |
| MS.gene27381.t1 | AT2G28260 | 37.445 | 227 | 118 | 6 | 2 | 209 | 363 | 584 | 8.65e-31 | 119 |
| MS.gene27381.t1 | AT5G14870 | 33.750 | 240 | 134 | 6 | 6 | 225 | 385 | 619 | 2.86e-30 | 118 |
| MS.gene27381.t1 | AT5G54250 | 34.513 | 226 | 131 | 6 | 1 | 211 | 427 | 650 | 1.68e-29 | 115 |
| MS.gene27381.t1 | AT5G54250 | 34.513 | 226 | 131 | 6 | 1 | 211 | 427 | 650 | 1.68e-29 | 115 |
| MS.gene27381.t1 | AT5G54250 | 34.513 | 226 | 131 | 6 | 1 | 211 | 427 | 650 | 1.68e-29 | 115 |
| MS.gene27381.t1 | AT2G46430 | 35.510 | 245 | 128 | 10 | 1 | 219 | 408 | 648 | 7.37e-29 | 114 |
| MS.gene27381.t1 | AT2G46430 | 35.510 | 245 | 128 | 10 | 1 | 219 | 408 | 648 | 7.37e-29 | 114 |
| MS.gene27381.t1 | AT5G54250 | 34.222 | 225 | 128 | 7 | 1 | 211 | 427 | 645 | 1.59e-26 | 107 |
| MS.gene27381.t1 | AT5G15410 | 33.333 | 225 | 119 | 7 | 1 | 203 | 462 | 677 | 1.37e-22 | 95.9 |
| MS.gene27381.t1 | AT5G15410 | 33.333 | 225 | 119 | 7 | 1 | 203 | 461 | 676 | 1.47e-22 | 95.9 |
| MS.gene27381.t1 | AT2G46450 | 30.000 | 230 | 146 | 5 | 1 | 218 | 343 | 569 | 5.17e-22 | 94.4 |
| MS.gene27381.t1 | AT2G46450 | 30.000 | 230 | 146 | 5 | 1 | 218 | 330 | 556 | 5.22e-22 | 94.4 |
| MS.gene27381.t1 | AT2G46450 | 30.000 | 230 | 146 | 5 | 1 | 218 | 366 | 592 | 5.83e-22 | 94.0 |
| MS.gene27381.t1 | AT2G46450 | 30.000 | 230 | 146 | 5 | 1 | 218 | 366 | 592 | 5.83e-22 | 94.0 |
| MS.gene27381.t1 | AT2G46440 | 32.271 | 251 | 128 | 10 | 1 | 219 | 188 | 428 | 7.34e-20 | 87.8 |
| MS.gene27381.t1 | AT2G46440 | 32.271 | 251 | 128 | 10 | 1 | 219 | 366 | 606 | 1.68e-19 | 87.0 |
Find 53 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTTATTGTGCGTGGAAAAT+TGG | 0.274539 | 6.4:+50597537 | MS.gene27381:CDS |
| GCTTACCAGAAGAACTTAAA+AGG | 0.284129 | 6.4:+50596833 | MS.gene27381:CDS |
| CACTTGCAGTAAACAGAATT+CGG | 0.289321 | 6.4:+50598475 | MS.gene27381:CDS |
| ATGTCTGTCTGGAGATCTTC+TGG | 0.319596 | 6.4:-50597047 | None:intergenic |
| AAGGGGTGTTCCTGAGAAAA+TGG | 0.334550 | 6.4:+50597010 | MS.gene27381:CDS |
| ATACAGACGACATACATTAA+AGG | 0.335319 | 6.4:+50597472 | MS.gene27381:CDS |
| TTAGGTATGAATCACCTTAC+TGG | 0.364609 | 6.4:+50598448 | MS.gene27381:intron |
| TCACGAATGGCGTCTAAGAT+AGG | 0.365376 | 6.4:-50597442 | None:intergenic |
| AGGAAGTAAATTTCTCAGTC+AGG | 0.403042 | 6.4:+50597492 | MS.gene27381:CDS |
| TGTTCCTGAGAAAATGGTTC+TGG | 0.409988 | 6.4:+50597016 | MS.gene27381:CDS |
| GTTCTTCTGGTAAGCAATGA+TGG | 0.420523 | 6.4:-50596825 | None:intergenic |
| CGTCGGAGAGGATGGAATTC+CGG | 0.422155 | 6.4:+50597564 | MS.gene27381:CDS |
| TTCGGGTTGCATGGAGATAC+AGG | 0.422208 | 6.4:+50598493 | MS.gene27381:CDS |
| TAGGGTCATCCTCAGCCATA+TGG | 0.436659 | 6.4:-50597423 | None:intergenic |
| GGCAGGCTGAACGGTATAGT+TGG | 0.446424 | 6.4:+50596979 | MS.gene27381:CDS |
| TCCCCTTCTGATAACGGAAC+CGG | 0.459445 | 6.4:-50597583 | None:intergenic |
| GAAACCACATCGATTAATCA+TGG | 0.466495 | 6.4:-50597731 | None:intergenic |
| GCAGGCTGAACGGTATAGTT+GGG | 0.472885 | 6.4:+50596980 | MS.gene27381:CDS |
| TTGGAGAGCGTCGGAGAGGA+TGG | 0.482934 | 6.4:+50597556 | MS.gene27381:CDS |
| GTCATGCAGGAGAGTGCGGC+AGG | 0.485642 | 6.4:+50596962 | MS.gene27381:intron |
| TATTAACAAATTTGAAGAGA+TGG | 0.501093 | 6.4:-50597076 | None:intergenic |
| GTATAGTTGGGCTGCAACAA+GGG | 0.505727 | 6.4:+50596992 | MS.gene27381:CDS |
| CAAGCATCCCCTTCTGATAA+CGG | 0.505912 | 6.4:-50597589 | None:intergenic |
| GATGGCGTCTTATGTCTGTC+TGG | 0.521052 | 6.4:-50597058 | None:intergenic |
| ACTTGCAGTAAACAGAATTC+GGG | 0.523626 | 6.4:+50598476 | MS.gene27381:CDS |
| TAAACAGAATTCGGGTTGCA+TGG | 0.527023 | 6.4:+50598484 | MS.gene27381:CDS |
| GGTATAGTTGGGCTGCAACA+AGG | 0.533253 | 6.4:+50596991 | MS.gene27381:CDS |
| CACGAATGGCGTCTAAGATA+GGG | 0.533961 | 6.4:-50597441 | None:intergenic |
| CATCGATTAATCATGGTTTG+AGG | 0.537968 | 6.4:-50597724 | None:intergenic |
| AGGATAGAAATGCAACTTAG+AGG | 0.538796 | 6.4:+50596778 | None:intergenic |
| GGGTGGTCTTGTACAGAAGA+TGG | 0.547676 | 6.4:+50597513 | MS.gene27381:CDS |
| GGAAGTAAATTTCTCAGTCA+GGG | 0.548088 | 6.4:+50597493 | MS.gene27381:CDS |
| AGGGTCATCCTCAGCCATAT+GGG | 0.559056 | 6.4:-50597422 | None:intergenic |
| TCCGGTTCCGTTATCAGAAG+GGG | 0.560727 | 6.4:+50597582 | MS.gene27381:CDS |
| GGAGAGTGCGGCAGGCTGAA+CGG | 0.561282 | 6.4:+50596970 | MS.gene27381:intron |
| ACTGCAAGTGATCTCCAGTA+AGG | 0.565458 | 6.4:-50598462 | None:intergenic |
| ATTCCGGTTCCGTTATCAGA+AGG | 0.567052 | 6.4:+50597580 | MS.gene27381:CDS |
| TTATCAAACATTGATGCCAT+AGG | 0.570396 | 6.4:+50598554 | MS.gene27381:CDS |
| CTGTATTAGTCTCTCACGAA+TGG | 0.579807 | 6.4:-50597455 | None:intergenic |
| GATGTGGTTTCTCTTGCAGA+AGG | 0.583374 | 6.4:+50597743 | MS.gene27381:CDS |
| ATCTGTCATGCAGGAGAGTG+CGG | 0.584547 | 6.4:+50596958 | MS.gene27381:intron |
| GTGGTGAAGAACTTCTAAGA+TGG | 0.591370 | 6.4:+50597611 | MS.gene27381:CDS |
| TTCCGGTTCCGTTATCAGAA+GGG | 0.604864 | 6.4:+50597581 | MS.gene27381:CDS |
| AAGATGGTGTTTATTGTGCG+TGG | 0.607353 | 6.4:+50597529 | MS.gene27381:CDS |
| TATAGTTGGGCTGCAACAAG+GGG | 0.617263 | 6.4:+50596993 | MS.gene27381:CDS |
| CGTGGAAAATTGGAGAGCGT+CGG | 0.618147 | 6.4:+50597547 | MS.gene27381:CDS |
| GAACAGTCTTCTGAAAGCAA+AGG | 0.627118 | 6.4:+50597640 | MS.gene27381:CDS |
| TTATCAGAAGGGGATGCTTG+TGG | 0.631551 | 6.4:+50597592 | MS.gene27381:CDS |
| CAAACCATGATTAATCGATG+TGG | 0.644618 | 6.4:+50597727 | MS.gene27381:CDS |
| GAGGTCGTGATGTTGAGCAA+TGG | 0.676062 | 6.4:+50596797 | MS.gene27381:CDS |
| AAAATTGGAGAGCGTCGGAG+AGG | 0.681877 | 6.4:+50597552 | MS.gene27381:CDS |
| AGTAAATTTCTCAGTCAGGG+TGG | 0.692049 | 6.4:+50597496 | MS.gene27381:CDS |
| TGTTTGATAATCATTATGTG+TGG | 0.718297 | 6.4:-50598541 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATGGATTACTTATTA+TGG | + | chr6.4:50596914-50596933 | MS.gene27381:intron | 15.0% |
| !! | AAAACACATCTATCAAATAA+TGG | + | chr6.4:50596900-50596919 | MS.gene27381:intron | 20.0% |
| !! | AAATGCAATTGAATCATAAA+AGG | - | chr6.4:50597995-50598014 | None:intergenic | 20.0% |
| !! | AATGCAATTGAATCATAAAA+GGG | - | chr6.4:50597994-50598013 | None:intergenic | 20.0% |
| !! | TATTAACAAATTTGAAGAGA+TGG | - | chr6.4:50597079-50597098 | None:intergenic | 20.0% |
| !! | TTTATGATTCAATTGCATTT+CGG | + | chr6.4:50597995-50598014 | MS.gene27381:intron | 20.0% |
| !!! | ATTATTTGATAGATGTGTTT+TGG | - | chr6.4:50596901-50596920 | None:intergenic | 20.0% |
| !!! | TGGTTTTGATAATAGATATT+AGG | - | chr6.4:50598388-50598407 | None:intergenic | 20.0% |
| !!! | TTAAGTCGATTTTATTTATC+TGG | + | chr6.4:50597134-50597153 | MS.gene27381:intron | 20.0% |
| ! | AATTGTTATGAGTTCCATAT+TGG | - | chr6.4:50598325-50598344 | None:intergenic | 25.0% |
| ! | ACTTATTATGGTACAAATGA+TGG | + | chr6.4:50596926-50596945 | MS.gene27381:intron | 25.0% |
| ! | AGAATGTGTTTATAAGTGAT+GGG | - | chr6.4:50598081-50598100 | None:intergenic | 25.0% |
| ! | ATATCTTATCTCATCCAATA+TGG | + | chr6.4:50598308-50598327 | MS.gene27381:intron | 25.0% |
| ! | TAAAACAATGACGCATTAAA+AGG | + | chr6.4:50597311-50597330 | MS.gene27381:intron | 25.0% |
| ! | TATGACAATATGTATTCAAG+TGG | - | chr6.4:50598291-50598310 | None:intergenic | 25.0% |
| ! | TCTCTGACGTAATAATTATT+TGG | + | chr6.4:50598410-50598429 | MS.gene27381:intron | 25.0% |
| !! | TGTTTGATAATCATTATGTG+TGG | - | chr6.4:50598544-50598563 | None:intergenic | 25.0% |
| !! | TTTTCTCATTCAACTTCTAT+TGG | - | chr6.4:50597250-50597269 | None:intergenic | 25.0% |
| ACAATATGTATTCAAGTGGT+GGG | - | chr6.4:50598287-50598306 | None:intergenic | 30.0% | |
| ATACAGACGACATACATTAA+AGG | + | chr6.4:50597472-50597491 | MS.gene27381:CDS | 30.0% | |
| ATACTATATATCTGTCATGC+AGG | + | chr6.4:50596949-50596968 | MS.gene27381:intron | 30.0% | |
| ATTTGCATAAACCTTTGTCT+TGG | - | chr6.4:50597279-50597298 | None:intergenic | 30.0% | |
| GAAAACAATTTCCAAGACAA+AGG | + | chr6.4:50597265-50597284 | MS.gene27381:intron | 30.0% | |
| GAGAATGTGTTTATAAGTGA+TGG | - | chr6.4:50598082-50598101 | None:intergenic | 30.0% | |
| GCATGTAAATAGCATACTTA+TGG | - | chr6.4:50598366-50598385 | None:intergenic | 30.0% | |
| GTGTTCTTAACCTTATAAGA+CGG | - | chr6.4:50598059-50598078 | None:intergenic | 30.0% | |
| TAACTCAACCATTAAAACAC+TGG | + | chr6.4:50598236-50598255 | MS.gene27381:intron | 30.0% | |
| TAATTATTACGTCAGAGACA+TGG | - | chr6.4:50598408-50598427 | None:intergenic | 30.0% | |
| TGATGATCTAACATTTCTAC+AGG | + | chr6.4:50597387-50597406 | MS.gene27381:intron | 30.0% | |
| ! | ACCATCTTAGAATTTGAGTT+AGG | + | chr6.4:50598211-50598230 | MS.gene27381:intron | 30.0% |
| !! | CTCAAATTCTAAGATGGTAT+CGG | - | chr6.4:50598209-50598228 | None:intergenic | 30.0% |
| !! | TGGTTGTTTTGTGTTATGTT+AGG | + | chr6.4:50598430-50598449 | MS.gene27381:intron | 30.0% |
| !!! | AGTGTTTTAATGGTTGAGTT+AGG | - | chr6.4:50598237-50598256 | None:intergenic | 30.0% |
| !!! | TCATACCTTTTAAGTTCTTC+TGG | - | chr6.4:50596841-50596860 | None:intergenic | 30.0% |
| AAAAATAAAGCTTCACAGCC+AGG | + | chr6.4:50597769-50597788 | MS.gene27381:intron | 35.0% | |
| AAAATAAAGCTTCACAGCCA+GGG | + | chr6.4:50597770-50597789 | MS.gene27381:intron | 35.0% | |
| ACTTGCAGTAAACAGAATTC+GGG | + | chr6.4:50598476-50598495 | MS.gene27381:CDS | 35.0% | |
| AGGAAGTAAATTTCTCAGTC+AGG | + | chr6.4:50597492-50597511 | MS.gene27381:CDS | 35.0% | |
| AGTAAGATGCTTAACCAATG+TGG | + | chr6.4:50597811-50597830 | MS.gene27381:intron | 35.0% | |
| ATTGGATTCCAACAAGTTAC+AGG | - | chr6.4:50597232-50597251 | None:intergenic | 35.0% | |
| CAAACCATGATTAATCGATG+TGG | + | chr6.4:50597727-50597746 | MS.gene27381:CDS | 35.0% | |
| CACTTGCAGTAAACAGAATT+CGG | + | chr6.4:50598475-50598494 | MS.gene27381:CDS | 35.0% | |
| GACAATATGTATTCAAGTGG+TGG | - | chr6.4:50598288-50598307 | None:intergenic | 35.0% | |
| GCTTACCAGAAGAACTTAAA+AGG | + | chr6.4:50596833-50596852 | MS.gene27381:CDS | 35.0% | |
| GGAAGTAAATTTCTCAGTCA+GGG | + | chr6.4:50597493-50597512 | MS.gene27381:CDS | 35.0% | |
| GGCGCATGAATATAAATGAT+GGG | + | chr6.4:50598162-50598181 | MS.gene27381:intron | 35.0% | |
| GTTTATTGTGCGTGGAAAAT+TGG | + | chr6.4:50597537-50597556 | MS.gene27381:CDS | 35.0% | |
| TATTGACACAAAGACAGAAG+GGG | - | chr6.4:50597955-50597974 | None:intergenic | 35.0% | |
| TCAGACCTTATCTCTTAAGT+GGG | + | chr6.4:50598100-50598119 | MS.gene27381:intron | 35.0% | |
| TTAGGTATGAATCACCTTAC+TGG | + | chr6.4:50598448-50598467 | MS.gene27381:intron | 35.0% | |
| TTATAACTCCTTGAACACGA+GGG | - | chr6.4:50597904-50597923 | None:intergenic | 35.0% | |
| ! | CATCGATTAATCATGGTTTG+AGG | - | chr6.4:50597727-50597746 | None:intergenic | 35.0% |
| ! | CTCTTTTCGCAAGATATTTG+CGG | + | chr6.4:50597876-50597895 | MS.gene27381:intron | 35.0% |
| ! | GAAACCACATCGATTAATCA+TGG | - | chr6.4:50597734-50597753 | None:intergenic | 35.0% |
| ! | GCATGAATATAAATGATGGG+TGG | + | chr6.4:50598165-50598184 | MS.gene27381:intron | 35.0% |
| ! | GCCTAACTCAAATTCTAAGA+TGG | - | chr6.4:50598215-50598234 | None:intergenic | 35.0% |
| ! | GTATTGACACAAAGACAGAA+GGG | - | chr6.4:50597956-50597975 | None:intergenic | 35.0% |
| ! | TTTTCCAGAACCATTTTCTC+AGG | - | chr6.4:50597023-50597042 | None:intergenic | 35.0% |
| !! | AGTATTGACACAAAGACAGA+AGG | - | chr6.4:50597957-50597976 | None:intergenic | 35.0% |
| !! | CATTAAAACACTGGCTTGAT+AGG | + | chr6.4:50598245-50598264 | MS.gene27381:intron | 35.0% |
| !! | CTATCAAGCCAGTGTTTTAA+TGG | - | chr6.4:50598247-50598266 | None:intergenic | 35.0% |
| !!! | AATTTTTTCCCATATGGCTG+AGG | + | chr6.4:50597414-50597433 | MS.gene27381:CDS | 35.0% |
| !!! | GGTTCGAATTTTTTCCCATA+TGG | + | chr6.4:50597408-50597427 | MS.gene27381:intron | 35.0% |
| AAGATGCTTAACCAATGTGG+AGG | + | chr6.4:50597814-50597833 | MS.gene27381:intron | 40.0% | |
| AAGATGGTGTTTATTGTGCG+TGG | + | chr6.4:50597529-50597548 | MS.gene27381:CDS | 40.0% | |
| ACAATAGATTCGGGCGTATT+AGG | - | chr6.4:50598138-50598157 | None:intergenic | 40.0% | |
| AGTAAATTTCTCAGTCAGGG+TGG | + | chr6.4:50597496-50597515 | MS.gene27381:CDS | 40.0% | |
| CAAGTCCCACTTAAGAGATA+AGG | - | chr6.4:50598108-50598127 | None:intergenic | 40.0% | |
| CGGCGCATGAATATAAATGA+TGG | + | chr6.4:50598161-50598180 | MS.gene27381:intron | 40.0% | |
| CTCAGACCTTATCTCTTAAG+TGG | + | chr6.4:50598099-50598118 | MS.gene27381:intron | 40.0% | |
| CTCGTGTTCAAGGAGTTATA+AGG | + | chr6.4:50597903-50597922 | MS.gene27381:intron | 40.0% | |
| CTTATAACTCCTTGAACACG+AGG | - | chr6.4:50597905-50597924 | None:intergenic | 40.0% | |
| CTTATCTCTTAAGTGGGACT+TGG | + | chr6.4:50598106-50598125 | MS.gene27381:intron | 40.0% | |
| CTTCTTCAATGTCTTTAGCG+CGG | - | chr6.4:50597850-50597869 | None:intergenic | 40.0% | |
| GATGGGTGGTTCAAATCAAT+AGG | + | chr6.4:50598179-50598198 | MS.gene27381:intron | 40.0% | |
| GTTCTTCTGGTAAGCAATGA+TGG | - | chr6.4:50596828-50596847 | None:intergenic | 40.0% | |
| TGTTCCTGAGAAAATGGTTC+TGG | + | chr6.4:50597016-50597035 | MS.gene27381:CDS | 40.0% | |
| ! | CTGTATTAGTCTCTCACGAA+TGG | - | chr6.4:50597458-50597477 | None:intergenic | 40.0% |
| ! | GAACAGTCTTCTGAAAGCAA+AGG | + | chr6.4:50597640-50597659 | MS.gene27381:CDS | 40.0% |
| ! | GATTGCGACAACTTCTTTTG+AGG | - | chr6.4:50597696-50597715 | None:intergenic | 40.0% |
| ! | GTGGTGAAGAACTTCTAAGA+TGG | + | chr6.4:50597611-50597630 | MS.gene27381:CDS | 40.0% |
| !! | AAAACACTGGCTTGATAGGT+GGG | + | chr6.4:50598249-50598268 | MS.gene27381:intron | 40.0% |
| !! | TAAAACACTGGCTTGATAGG+TGG | + | chr6.4:50598248-50598267 | MS.gene27381:intron | 40.0% |
| !! | TAAACAGAATTCGGGTTGCA+TGG | + | chr6.4:50598484-50598503 | MS.gene27381:CDS | 40.0% |
| !! | TGCATTTCGGTGTTGTGTTT+GGG | + | chr6.4:50598008-50598027 | MS.gene27381:intron | 40.0% |
| !! | TTGCATTTCGGTGTTGTGTT+TGG | + | chr6.4:50598007-50598026 | MS.gene27381:intron | 40.0% |
| ACACAAAGACAGAAGGGGAA+TGG | - | chr6.4:50597950-50597969 | None:intergenic | 45.0% | |
| ACCTTATAAGACGGCTCTGT+AGG | - | chr6.4:50598050-50598069 | None:intergenic | 45.0% | |
| ACGCCCGAATCTATTGTACT+CGG | + | chr6.4:50598141-50598160 | MS.gene27381:intron | 45.0% | |
| ACTGCAAGTGATCTCCAGTA+AGG | - | chr6.4:50598465-50598484 | None:intergenic | 45.0% | |
| ATGTCTGTCTGGAGATCTTC+TGG | - | chr6.4:50597050-50597069 | None:intergenic | 45.0% | |
| ATTCCGGTTCCGTTATCAGA+AGG | + | chr6.4:50597580-50597599 | MS.gene27381:CDS | 45.0% | |
| CAAGCATCCCCTTCTGATAA+CGG | - | chr6.4:50597592-50597611 | None:intergenic | 45.0% | |
| CACAAAGACAGAAGGGGAAT+GGG | - | chr6.4:50597949-50597968 | None:intergenic | 45.0% | |
| CCTTATAAGACGGCTCTGTA+GGG | - | chr6.4:50598049-50598068 | None:intergenic | 45.0% | |
| GAGTGAAAATGCCTCCACAT+TGG | - | chr6.4:50597828-50597847 | None:intergenic | 45.0% | |
| GATGTGGTTTCTCTTGCAGA+AGG | + | chr6.4:50597743-50597762 | MS.gene27381:CDS | 45.0% | |
| GTATAGTTGGGCTGCAACAA+GGG | + | chr6.4:50596992-50597011 | MS.gene27381:CDS | 45.0% | |
| TATAGTTGGGCTGCAACAAG+GGG | + | chr6.4:50596993-50597012 | MS.gene27381:CDS | 45.0% | |
| TGCGCCGAGTACAATAGATT+CGG | - | chr6.4:50598148-50598167 | None:intergenic | 45.0% | |
| TTATCAGAAGGGGATGCTTG+TGG | + | chr6.4:50597592-50597611 | MS.gene27381:CDS | 45.0% | |
| TTCCGGTTCCGTTATCAGAA+GGG | + | chr6.4:50597581-50597600 | MS.gene27381:CDS | 45.0% | |
| ! | CACGAATGGCGTCTAAGATA+GGG | - | chr6.4:50597444-50597463 | None:intergenic | 45.0% |
| ! | TCACGAATGGCGTCTAAGAT+AGG | - | chr6.4:50597445-50597464 | None:intergenic | 45.0% |
| !! | AAGGGGTGTTCCTGAGAAAA+TGG | + | chr6.4:50597010-50597029 | MS.gene27381:CDS | 45.0% |
| !! | TTCGGTGTTGTGTTTGGGTT+GGG | + | chr6.4:50598013-50598032 | MS.gene27381:intron | 45.0% |
| !! | TTTCGGTGTTGTGTTTGGGT+TGG | + | chr6.4:50598012-50598031 | MS.gene27381:intron | 45.0% |
| !!! | AATTTTATTTAAAACATTAT+AGG | - | chr6.4:50597190-50597209 | None:intergenic | 5.0% |
| AAGACAGAAGGGGAATGGGA+TGG | - | chr6.4:50597945-50597964 | None:intergenic | 50.0% | |
| AGGGTCATCCTCAGCCATAT+GGG | - | chr6.4:50597425-50597444 | None:intergenic | 50.0% | |
| ATCTGTCATGCAGGAGAGTG+CGG | + | chr6.4:50596958-50596977 | MS.gene27381:intron | 50.0% | |
| CCCTACAGAGCCGTCTTATA+AGG | + | chr6.4:50598046-50598065 | MS.gene27381:intron | 50.0% | |
| GAGGTCGTGATGTTGAGCAA+TGG | + | chr6.4:50596797-50596816 | MS.gene27381:CDS | 50.0% | |
| GATGGCGTCTTATGTCTGTC+TGG | - | chr6.4:50597061-50597080 | None:intergenic | 50.0% | |
| GCAGGCTGAACGGTATAGTT+GGG | + | chr6.4:50596980-50596999 | MS.gene27381:CDS | 50.0% | |
| GCGCCGAGTACAATAGATTC+GGG | - | chr6.4:50598147-50598166 | None:intergenic | 50.0% | |
| GGTATAGTTGGGCTGCAACA+AGG | + | chr6.4:50596991-50597010 | MS.gene27381:CDS | 50.0% | |
| TAGGGTCATCCTCAGCCATA+TGG | - | chr6.4:50597426-50597445 | None:intergenic | 50.0% | |
| TCCGGTTCCGTTATCAGAAG+GGG | + | chr6.4:50597582-50597601 | MS.gene27381:CDS | 50.0% | |
| TTCTGTCGCTAGTCAAACCC+TGG | - | chr6.4:50597790-50597809 | None:intergenic | 50.0% | |
| ! | CGTGGAAAATTGGAGAGCGT+CGG | + | chr6.4:50597547-50597566 | MS.gene27381:CDS | 50.0% |
| !! | AAAATTGGAGAGCGTCGGAG+AGG | + | chr6.4:50597552-50597571 | MS.gene27381:CDS | 50.0% |
| !! | GGGTGGTCTTGTACAGAAGA+TGG | + | chr6.4:50597513-50597532 | MS.gene27381:CDS | 50.0% |
| !! | TCCCCTTCTGATAACGGAAC+CGG | - | chr6.4:50597586-50597605 | None:intergenic | 50.0% |
| !! | TTCGGGTTGCATGGAGATAC+AGG | + | chr6.4:50598493-50598512 | MS.gene27381:CDS | 50.0% |
| AGTTACAGGCAGACGCGTAG+AGG | - | chr6.4:50597218-50597237 | None:intergenic | 55.0% | |
| CGTCGGAGAGGATGGAATTC+CGG | + | chr6.4:50597564-50597583 | MS.gene27381:CDS | 55.0% | |
| GGCAGGCTGAACGGTATAGT+TGG | + | chr6.4:50596979-50596998 | MS.gene27381:CDS | 55.0% | |
| ! | CGCGTCTGCCTGTAACTTGT+TGG | + | chr6.4:50597221-50597240 | MS.gene27381:intron | 55.0% |
| TTGCGGAGCCCTCGTGTTCA+AGG | + | chr6.4:50597893-50597912 | MS.gene27381:intron | 60.0% | |
| !! | TTGGAGAGCGTCGGAGAGGA+TGG | + | chr6.4:50597556-50597575 | MS.gene27381:CDS | 60.0% |
| ! | GGAGAGTGCGGCAGGCTGAA+CGG | + | chr6.4:50596970-50596989 | MS.gene27381:intron | 65.0% |
| ! | GTCATGCAGGAGAGTGCGGC+AGG | + | chr6.4:50596962-50596981 | MS.gene27381:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 50596787 | 50598575 | 50596787 | ID=MS.gene27381 |
| chr6.4 | mRNA | 50596787 | 50598575 | 50596787 | ID=MS.gene27381.t1;Parent=MS.gene27381 |
| chr6.4 | exon | 50596787 | 50596854 | 50596787 | ID=MS.gene27381.t1.exon1;Parent=MS.gene27381.t1 |
| chr6.4 | CDS | 50596787 | 50596854 | 50596787 | ID=cds.MS.gene27381.t1;Parent=MS.gene27381.t1 |
| chr6.4 | exon | 50596971 | 50597100 | 50596971 | ID=MS.gene27381.t1.exon2;Parent=MS.gene27381.t1 |
| chr6.4 | CDS | 50596971 | 50597100 | 50596971 | ID=cds.MS.gene27381.t1;Parent=MS.gene27381.t1 |
| chr6.4 | exon | 50597409 | 50597764 | 50597409 | ID=MS.gene27381.t1.exon3;Parent=MS.gene27381.t1 |
| chr6.4 | CDS | 50597409 | 50597764 | 50597409 | ID=cds.MS.gene27381.t1;Parent=MS.gene27381.t1 |
| chr6.4 | exon | 50598452 | 50598575 | 50598452 | ID=MS.gene27381.t1.exon4;Parent=MS.gene27381.t1 |
| chr6.4 | CDS | 50598452 | 50598575 | 50598452 | ID=cds.MS.gene27381.t1;Parent=MS.gene27381.t1 |
| Gene Sequence |
| Protein sequence |
