AlfalfaGEDB | MS.gene27678

M. sativa cultivar XinJiangDaYe / MS.gene27678

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27678.t1	XP_003623880.1	100	62	0	0	1	62	1	62	3.00E-28	133.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27678.t1	G7L2K4	100.0	62	0	0	1	62	1	62	2.2e-28	133.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049857	MS.gene27678	0.808829	2.55E-50	-1.69E-46
MS.gene050277	MS.gene27678	0.815118	1.09E-51	-1.69E-46
MS.gene050320	MS.gene27678	0.827533	1.48E-54	-1.69E-46
MS.gene050838	MS.gene27678	0.84154	4.47E-58	-1.69E-46
MS.gene051746	MS.gene27678	0.835555	1.57E-56	-1.69E-46
MS.gene051748	MS.gene27678	0.848825	4.79E-60	-1.69E-46
MS.gene051756	MS.gene27678	0.85037	1.77E-60	-1.69E-46
MS.gene051757	MS.gene27678	0.807323	5.35E-50	-1.69E-46
MS.gene051850	MS.gene27678	0.848946	4.43E-60	-1.69E-46
MS.gene051903	MS.gene27678	0.843224	1.60E-58	-1.69E-46
MS.gene053109	MS.gene27678	0.84656	2.01E-59	-1.69E-46
MS.gene053952	MS.gene27678	0.800901	1.16E-48	-1.69E-46
MS.gene056432	MS.gene27678	0.825833	3.78E-54	-1.69E-46
MS.gene056992	MS.gene27678	0.809171	2.16E-50	-1.69E-46
MS.gene057057	MS.gene27678	0.814407	1.56E-51	-1.69E-46
MS.gene057094	MS.gene27678	0.851893	6.60E-61	-1.69E-46
MS.gene057234	MS.gene27678	0.863464	2.44E-64	-1.69E-46
MS.gene058055	MS.gene27678	0.840878	6.67E-58	-1.69E-46
MS.gene058238	MS.gene27678	0.804862	1.76E-49	-1.69E-46
MS.gene058906	MS.gene27678	0.845225	4.64E-59	-1.69E-46
MS.gene06001	MS.gene27678	0.80777	4.30E-50	-1.69E-46
MS.gene060465	MS.gene27678	0.801772	7.69E-49	-1.69E-46
MS.gene060702	MS.gene27678	0.860068	2.67E-63	-1.69E-46
MS.gene061145	MS.gene27678	0.850649	1.48E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27678.t1	MTR_7g076630	100.000	62	0	0	1	62	1	62	2.19e-41	129

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27678.t1	AT4G00585	83.051	59	9	1	4	62	2	59	7.42e-30	100

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGTTGCGTTTCCAGTGTTT+AGG	0.292140	7.2:+29108415	None:intergenic
GAGCCAATGGAGGAGATTTC+AGG	0.296342	7.2:-29108477	MS.gene27678:CDS
TGTTGCGTTTCCAGTGTTTA+GGG	0.348623	7.2:+29108416	None:intergenic
GAGATTTCAGGTACAAGGTT+TGG	0.375661	7.2:-29108465	MS.gene27678:CDS
TTTAGCTGAGAGTTTGAAGA+TGG	0.461581	7.2:+29108354	None:intergenic
CACGGTAATGGCGGAGCCAA+TGG	0.484941	7.2:-29108490	MS.gene27678:CDS
TAGCTGAGAGTTTGAAGATG+GGG	0.490985	7.2:+29108356	None:intergenic
GTACCTGAAATCTCCTCCAT+TGG	0.492681	7.2:+29108474	None:intergenic
AACACTGCCATCGCTATGTT+CGG	0.493133	7.2:-29108397	MS.gene27678:CDS
TGGAGGAGATTTCAGGTACA+AGG	0.512900	7.2:-29108470	MS.gene27678:CDS
TTAGCTGAGAGTTTGAAGAT+GGG	0.520876	7.2:+29108355	None:intergenic
GGTGGAGCAGATCACGGTAA+TGG	0.524664	7.2:-29108502	MS.gene27678:CDS
TACAAGGTTTGGAGTATGAC+TGG	0.533279	7.2:-29108454	MS.gene27678:CDS
CTCACTGTCGCCCTAAACAC+TGG	0.552111	7.2:-29108426	MS.gene27678:CDS
CAAACTCTCAGCTAAACTTG+AGG	0.608144	7.2:-29108347	None:intergenic
GGAGGTGGTGGAGCAGATCA+CGG	0.622572	7.2:-29108508	MS.gene27678:CDS
GGTAATGGCGGAGCCAATGG+AGG	0.641548	7.2:-29108487	MS.gene27678:CDS
GACGATACCGAACATAGCGA+TGG	0.647243	7.2:+29108390	None:intergenic
GGAGCAGATCACGGTAATGG+CGG	0.651187	7.2:-29108499	MS.gene27678:CDS
CAGTGTTTAGGGCGACAGTG+AGG	0.708119	7.2:+29108427	None:intergenic
AAGGTTTGGAGTATGACTGG+TGG	0.712549	7.2:-29108451	MS.gene27678:CDS
TGAAGATGGGGATACAAACG+AGG	0.746465	7.2:+29108368	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTAGCTGAGAGTTTGAAGAT+GGG	+	chr7.2:29108507-29108526	None:intergenic	35.0%
	TTTAGCTGAGAGTTTGAAGA+TGG	+	chr7.2:29108508-29108527	None:intergenic	35.0%
	TAGCTGAGAGTTTGAAGATG+GGG	+	chr7.2:29108506-29108525	None:intergenic	40.0%
	TGTTGCGTTTCCAGTGTTTA+GGG	+	chr7.2:29108446-29108465	None:intergenic	40.0%
!	GAGATTTCAGGTACAAGGTT+TGG	-	chr7.2:29108394-29108413	MS.gene27678:CDS	40.0%
!	TACAAGGTTTGGAGTATGAC+TGG	-	chr7.2:29108405-29108424	MS.gene27678:CDS	40.0%
	AACACTGCCATCGCTATGTT+CGG	-	chr7.2:29108462-29108481	MS.gene27678:CDS	45.0%
	GTACCTGAAATCTCCTCCAT+TGG	+	chr7.2:29108388-29108407	None:intergenic	45.0%
	GTGTTGCGTTTCCAGTGTTT+AGG	+	chr7.2:29108447-29108466	None:intergenic	45.0%
	TGAAGATGGGGATACAAACG+AGG	+	chr7.2:29108494-29108513	None:intergenic	45.0%
!	AAGGTTTGGAGTATGACTGG+TGG	-	chr7.2:29108408-29108427	MS.gene27678:CDS	45.0%
!	TGGAGGAGATTTCAGGTACA+AGG	-	chr7.2:29108389-29108408	MS.gene27678:CDS	45.0%
	GACGATACCGAACATAGCGA+TGG	+	chr7.2:29108472-29108491	None:intergenic	50.0%
	GAGCCAATGGAGGAGATTTC+AGG	-	chr7.2:29108382-29108401	MS.gene27678:CDS	50.0%
	CTCACTGTCGCCCTAAACAC+TGG	-	chr7.2:29108433-29108452	MS.gene27678:CDS	55.0%
	GGTGGAGCAGATCACGGTAA+TGG	-	chr7.2:29108357-29108376	MS.gene27678:CDS	55.0%
!	CAGTGTTTAGGGCGACAGTG+AGG	+	chr7.2:29108435-29108454	None:intergenic	55.0%
!	GGAGCAGATCACGGTAATGG+CGG	-	chr7.2:29108360-29108379	MS.gene27678:CDS	55.0%
!	CACGGTAATGGCGGAGCCAA+TGG	-	chr7.2:29108369-29108388	MS.gene27678:CDS	60.0%
!	GGTAATGGCGGAGCCAATGG+AGG	-	chr7.2:29108372-29108391	MS.gene27678:CDS	60.0%
!!	GGAGGTGGTGGAGCAGATCA+CGG	-	chr7.2:29108351-29108370	MS.gene27678:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	29108348	29108533	29108348	ID=MS.gene27678
chr7.2	mRNA	29108348	29108533	29108348	ID=MS.gene27678.t1;Parent=MS.gene27678
chr7.2	exon	29108348	29108533	29108348	ID=MS.gene27678.t1.exon1;Parent=MS.gene27678.t1
chr7.2	CDS	29108348	29108533	29108348	ID=cds.MS.gene27678.t1;Parent=MS.gene27678.t1

Gene Sequence	>MS.gene27678 ATGGGAGGTGGTGGAGCAGATCACGGTAATGGCGGAGCCAATGGAGGAGATTTCAGGTACAAGGTTTGGAGTATGACTGGTGGTCCTCACTGTCGCCCTAAACACTGGAAACGCAACACTGCCATCGCTATGTTCGGTATCGTCCTCGTTTGTATCCCCATCTTCAAACTCTCAGCTAAACTTGAG
Protein sequence	>MS.gene27678.t1 MGGGGADHGNGGANGGDFRYKVWSMTGGPHCRPKHWKRNTAIAMFGIVLVCIPIFKLSAKLE